JP2016506743A5 - - Google Patents
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- JP2016506743A5 JP2016506743A5 JP2015556536A JP2015556536A JP2016506743A5 JP 2016506743 A5 JP2016506743 A5 JP 2016506743A5 JP 2015556536 A JP2015556536 A JP 2015556536A JP 2015556536 A JP2015556536 A JP 2015556536A JP 2016506743 A5 JP2016506743 A5 JP 2016506743A5
- Authority
- JP
- Japan
- Prior art keywords
- glucose
- seq
- steviol
- amino acid
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- QFVOYBUQQBFCRH-UHFFFAOYSA-N Steviol Natural products C1CC2(C3)CC(=C)C3(O)CCC2C2(C)C1C(C)(C(O)=O)CCC2 QFVOYBUQQBFCRH-UHFFFAOYSA-N 0.000 claims description 32
- 244000005700 microbiome Species 0.000 claims description 32
- 229940032084 steviol Drugs 0.000 claims description 32
- QFVOYBUQQBFCRH-VQSWZGCSSA-N steviol Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)CC1)C[C@H]2[C@@]2(C)[C@H]1[C@](C)(C(O)=O)CCC2 QFVOYBUQQBFCRH-VQSWZGCSSA-N 0.000 claims description 31
- 108010078791 Carrier Proteins Proteins 0.000 claims description 21
- 108010043934 Sucrose synthase Proteins 0.000 claims description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 claims description 2
- -1 glycosyl Steviol glycosides Chemical class 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims 109
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- 239000004383 Steviol glycoside Substances 0.000 claims 87
- 235000019411 steviol glycoside Nutrition 0.000 claims 87
- 229930182488 steviol glycoside Natural products 0.000 claims 87
- 150000008144 steviol glycosides Chemical class 0.000 claims 86
- 108090000765 processed proteins & peptides Proteins 0.000 claims 78
- 229920001184 polypeptide Polymers 0.000 claims 77
- 102000004196 processed proteins & peptides Human genes 0.000 claims 77
- 125000003275 alpha amino acid group Chemical group 0.000 claims 58
- GSGVXNMGMKBGQU-PHESRWQRSA-N rebaudioside M Chemical compound C[C@@]12CCC[C@](C)([C@H]1CC[C@@]13CC(=C)[C@@](C1)(CC[C@@H]23)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)C(=O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GSGVXNMGMKBGQU-PHESRWQRSA-N 0.000 claims 27
- 108090000623 proteins and genes Proteins 0.000 claims 23
- 238000006206 glycosylation reaction Methods 0.000 claims 21
- 230000001279 glycosylating effect Effects 0.000 claims 20
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- RPYRMTHVSUWHSV-CUZJHZIBSA-N rebaudioside D Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RPYRMTHVSUWHSV-CUZJHZIBSA-N 0.000 claims 14
- 238000000034 method Methods 0.000 claims 13
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical group O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 claims 11
- 230000014509 gene expression Effects 0.000 claims 10
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- NUHSROFQTUXZQQ-UHFFFAOYSA-N isopentenyl diphosphate Chemical compound CC(=C)CCO[P@](O)(=O)OP(O)(O)=O NUHSROFQTUXZQQ-UHFFFAOYSA-N 0.000 claims 8
- 239000001512 FEMA 4601 Substances 0.000 claims 7
- HELXLJCILKEWJH-SEAGSNCFSA-N Rebaudioside A Natural products O=C(O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@]1(C)[C@@H]2[C@](C)([C@H]3[C@@]4(CC(=C)[C@@](O[C@H]5[C@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@H](O)[C@@H](CO)O5)(C4)CC3)CC2)CCC1 HELXLJCILKEWJH-SEAGSNCFSA-N 0.000 claims 7
- RLLCWNUIHGPAJY-RYBZXKSASA-N Rebaudioside E Natural products O=C(O[C@H]1[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O2)[C@@H](O)[C@@H](O)[C@H](CO)O1)[C@]1(C)[C@@H]2[C@@](C)([C@@H]3[C@@]4(CC(=C)[C@@](O[C@@H]5[C@@H](O[C@@H]6[C@@H](O)[C@H](O)[C@@H](O)[C@H](CO)O6)[C@H](O)[C@@H](O)[C@H](CO)O5)(C4)CC3)CC2)CCC1 RLLCWNUIHGPAJY-RYBZXKSASA-N 0.000 claims 7
- HELXLJCILKEWJH-UHFFFAOYSA-N entered according to Sigma 01432 Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC(C1OC2C(C(O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OC(CO)C(O)C(O)C1O HELXLJCILKEWJH-UHFFFAOYSA-N 0.000 claims 7
- 235000019203 rebaudioside A Nutrition 0.000 claims 7
- RLLCWNUIHGPAJY-SFUUMPFESA-N rebaudioside E Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RLLCWNUIHGPAJY-SFUUMPFESA-N 0.000 claims 7
- DRSKVOAJKLUMCL-MMUIXFKXSA-N u2n4xkx7hp Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(O)=O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O DRSKVOAJKLUMCL-MMUIXFKXSA-N 0.000 claims 7
- OINNEUNVOZHBOX-QIRCYJPOSA-K 2-trans,6-trans,10-trans-geranylgeranyl diphosphate(3-) Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CC\C(C)=C\COP([O-])(=O)OP([O-])([O-])=O OINNEUNVOZHBOX-QIRCYJPOSA-K 0.000 claims 6
- OINNEUNVOZHBOX-XBQSVVNOSA-N Geranylgeranyl diphosphate Natural products [P@](=O)(OP(=O)(O)O)(OC/C=C(\CC/C=C(\CC/C=C(\CC/C=C(\C)/C)/C)/C)/C)O OINNEUNVOZHBOX-XBQSVVNOSA-N 0.000 claims 6
- XCCTYIAWTASOJW-XVFCMESISA-N Uridine-5'-Diphosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 XCCTYIAWTASOJW-XVFCMESISA-N 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 5
- 239000011541 reaction mixture Substances 0.000 claims 5
- 239000006228 supernatant Substances 0.000 claims 5
- ONVABDHFQKWOSV-UHFFFAOYSA-N 16-Phyllocladene Natural products C1CC(C2)C(=C)CC32CCC2C(C)(C)CCCC2(C)C31 ONVABDHFQKWOSV-UHFFFAOYSA-N 0.000 claims 4
- VWFJDQUYCIWHTN-YFVJMOTDSA-N 2-trans,6-trans-farnesyl diphosphate Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CO[P@](O)(=O)OP(O)(O)=O VWFJDQUYCIWHTN-YFVJMOTDSA-N 0.000 claims 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 4
- 244000025254 Cannabis sativa Species 0.000 claims 4
- VWFJDQUYCIWHTN-FBXUGWQNSA-N Farnesyl diphosphate Natural products CC(C)=CCC\C(C)=C/CC\C(C)=C/COP(O)(=O)OP(O)(O)=O VWFJDQUYCIWHTN-FBXUGWQNSA-N 0.000 claims 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 4
- 101100028962 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDR1 gene Proteins 0.000 claims 4
- 241000544066 Stevia Species 0.000 claims 4
- 235000011180 diphosphates Nutrition 0.000 claims 4
- ONVABDHFQKWOSV-YQXATGRUSA-N ent-Kaur-16-ene Natural products C1C[C@@H](C2)C(=C)C[C@@]32CC[C@@H]2C(C)(C)CCC[C@@]2(C)[C@@H]31 ONVABDHFQKWOSV-YQXATGRUSA-N 0.000 claims 4
- NIKHGUQULKYIGE-SHAPNJEPSA-N ent-kaur-16-en-19-oic acid Chemical compound C([C@H]1C[C@]2(CC1=C)CC1)C[C@H]2[C@@]2(C)[C@H]1[C@](C)(C(O)=O)CCC2 NIKHGUQULKYIGE-SHAPNJEPSA-N 0.000 claims 4
- KWVKUAKMOIEELN-UHFFFAOYSA-N ent-kaur-16-en-19-oic acid Natural products CC1(C)CCCC2(C)C1CCC34CC(=C(C3)C(=O)O)CCC24 KWVKUAKMOIEELN-UHFFFAOYSA-N 0.000 claims 4
- ONVABDHFQKWOSV-HPUSYDDDSA-N ent-kaur-16-ene Chemical compound C1C[C@H](C2)C(=C)C[C@@]32CC[C@@H]2C(C)(C)CCC[C@@]2(C)[C@@H]31 ONVABDHFQKWOSV-HPUSYDDDSA-N 0.000 claims 4
- UIXMIBNGPQGJJJ-UHFFFAOYSA-N ent-kaurene Natural products CC1CC23CCC4C(CCCC4(C)C)C2CCC1C3 UIXMIBNGPQGJJJ-UHFFFAOYSA-N 0.000 claims 4
- NIKHGUQULKYIGE-UHFFFAOYSA-N kaurenoic acid Natural products C1CC2(CC3=C)CC3CCC2C2(C)C1C(C)(C(O)=O)CCC2 NIKHGUQULKYIGE-UHFFFAOYSA-N 0.000 claims 4
- 210000005253 yeast cell Anatomy 0.000 claims 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 3
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- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 claims 3
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- XPPKVPWEQAFLFU-UHFFFAOYSA-N diphosphoric acid Chemical compound OP(O)(=O)OP(O)(O)=O XPPKVPWEQAFLFU-UHFFFAOYSA-N 0.000 claims 2
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- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
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Families Citing this family (73)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9107436B2 (en) | 2011-02-17 | 2015-08-18 | Purecircle Sdn Bhd | Glucosylated steviol glycoside as a flavor modifier |
| US10696706B2 (en) | 2010-03-12 | 2020-06-30 | Purecircle Usa Inc. | Methods of preparing steviol glycosides and uses of the same |
| NZ604915A (en) | 2010-06-02 | 2014-10-31 | Evolva Nutrition Inc | Recombinant production of steviol glycosides |
| US11690391B2 (en) | 2011-02-17 | 2023-07-04 | Purecircle Sdn Bhd | Glucosylated steviol glycoside as a flavor modifier |
| US9771434B2 (en) | 2011-06-23 | 2017-09-26 | Purecircle Sdn Bhd | Products from stevia rebaudiana |
| SG10201606567TA (en) | 2011-08-08 | 2016-10-28 | Evolva Sa | Recombinant production of steviol glycosides |
| CN110358795B (zh) * | 2012-05-22 | 2024-12-03 | 谱赛科有限责任公司 | 高纯度的甜菊醇糖苷 |
| US9752174B2 (en) | 2013-05-28 | 2017-09-05 | Purecircle Sdn Bhd | High-purity steviol glycosides |
| CN105051195B (zh) | 2013-02-06 | 2019-09-06 | 埃沃尔瓦公司 | 用于提高莱鲍迪苷d和莱鲍迪苷m之产生的方法 |
| WO2014191581A2 (en) * | 2013-05-31 | 2014-12-04 | Dsm Ip Assets B.V. | Microorganisms for diterpene production |
| DK3714699T3 (da) | 2013-06-07 | 2025-01-20 | Purecircle Usa Inc | Stevia-ekstrakt indeholdende udvalgte steviolglycosider som aroma-, salt- og sødmeprofilmodifikator |
| US10952458B2 (en) | 2013-06-07 | 2021-03-23 | Purecircle Usa Inc | Stevia extract containing selected steviol glycosides as flavor, salty and sweetness profile modifier |
| MX2016000452A (es) * | 2013-07-15 | 2016-05-12 | Dsm Ip Assets Bv | Produccion de diterpeno. |
| US10370418B2 (en) * | 2014-05-20 | 2019-08-06 | Lawrence Livermore National Security, Llc | Engineered microorganisms having resistance to ionic liquids |
| BR112017002783A2 (pt) | 2014-08-11 | 2017-12-19 | Evolva Sa | produção de glicosídeos de esteviol em hospedeiros recombinantes |
| CN114794444A (zh) | 2014-09-02 | 2022-07-29 | 谱赛科美国股份有限公司 | 富含莱鲍迪苷d、e、n和/或o的甜菊提取物及其制备方法 |
| MY191735A (en) * | 2014-09-09 | 2022-07-13 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| DK3201351T3 (da) * | 2014-10-03 | 2021-02-22 | Conagen Inc | Kaloriefrie sødestoffer og fremgangsmåder til syntetisering |
| US10472661B2 (en) | 2014-10-08 | 2019-11-12 | Dsm Ip Assets B.V. | Method for increasing glycosylation of a composition comprising steviol glycosides |
| CA2968280A1 (en) * | 2014-11-24 | 2016-06-02 | Cargill, Incorporated | Glycoside compositions |
| US10711024B2 (en) | 2014-12-17 | 2020-07-14 | Cargill, Incorporated | Steviol glycoside compounds, compositions for oral ingestion or use, and method for enhancing steviol glycoside solubility |
| JP2018503388A (ja) | 2015-01-30 | 2018-02-08 | エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． | 組み換え宿主におけるステビオールグリコシドの生産 |
| CN107404911A (zh) * | 2015-02-10 | 2017-11-28 | 谱赛科美国股份有限公司 | 莱鲍迪苷m生物合成生产和回收方法 |
| CN115851470B (zh) | 2015-03-16 | 2025-09-02 | 帝斯曼知识产权资产管理有限公司 | Udp-糖基转移酶 |
| WO2016156616A1 (en) | 2015-04-03 | 2016-10-06 | Dsm Ip Assets B.V. | Steviol glycosides |
| JP6845159B2 (ja) | 2015-05-20 | 2021-03-17 | カーギル インコーポレイテッド | グリコシド組成物 |
| EP3302097B1 (en) | 2015-05-29 | 2022-03-23 | Cargill, Incorporated | Heat treatment to produce glycosides |
| US10815513B2 (en) | 2015-05-29 | 2020-10-27 | Cargill, Incorporated | Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom |
| CN107614677A (zh) * | 2015-05-29 | 2018-01-19 | 嘉吉公司 | 用于使用多阶段进料生产甜菊醇糖苷的发酵方法 |
| CN105154348B (zh) * | 2015-08-05 | 2018-05-04 | 大连理工大学 | 一种提高酿酒酵母对纤维素水解液抑制物耐受性的方法 |
| WO2017024313A1 (en) | 2015-08-06 | 2017-02-09 | Cargill, Incorporated | Fermentation methods for producing steviol glycosides |
| AU2016307066A1 (en) * | 2015-08-07 | 2018-02-08 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| MY195726A (en) * | 2015-08-13 | 2023-02-07 | Dsm Ip Assets Bv | Steviol Glycoside Transport |
| WO2017025649A1 (en) * | 2015-08-13 | 2017-02-16 | Dsm Ip Assets B.V. | Steviol glycoside transport |
| EP3337893B1 (en) | 2015-08-21 | 2023-07-19 | Manus Bio Inc. | Increasing productivity of e. coli host cells that functionally express p450 enzymes |
| US9765117B2 (en) | 2015-08-24 | 2017-09-19 | Romek Figa | Peptides for treating cancer |
| EP3141610A1 (en) * | 2015-09-12 | 2017-03-15 | Jennewein Biotechnologie GmbH | Production of human milk oligosaccharides in microbial hosts with engineered import / export |
| PL3367816T3 (pl) | 2015-10-26 | 2024-05-06 | Purecircle Usa Inc. | Kompozycje glikozydów stewiolowych |
| WO2017106577A1 (en) | 2015-12-15 | 2017-06-22 | Purecircle Usa Inc. | Steviol glycoside compositions |
| CN109154010A (zh) | 2016-03-11 | 2019-01-04 | 埃沃尔瓦公司 | 在重组宿主中生产甜菊醇糖苷 |
| SG11201808856TA (en) | 2016-04-13 | 2018-11-29 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| WO2017198682A1 (en) | 2016-05-16 | 2017-11-23 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| KR20190018512A (ko) | 2016-06-15 | 2019-02-22 | 코덱시스, 인코포레이티드 | 조작된 베타-글루코시다아제 및 글루코실화 방법 |
| JP2019517807A (ja) * | 2016-06-15 | 2019-06-27 | コデクシス， インコーポレイテッド | ステビオールグリコシドおよびグルコース１−リン酸を含む他の化合物の酵素的グルコシル化 |
| CN110049687A (zh) * | 2016-06-17 | 2019-07-23 | 嘉吉公司 | 用于口服摄取或使用的甜菊醇糖苷组合物 |
| ES2906310T3 (es) | 2016-08-12 | 2022-04-18 | Amyris Inc | Glicosiltransferasa dependiente de UDP para la producción de alta eficiencia de rebaudiósidos |
| EP3535406A1 (en) * | 2016-11-07 | 2019-09-11 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| CN108085262B (zh) * | 2016-11-23 | 2022-08-05 | 中国科学院天津工业生物技术研究所 | 对萜类或含萜精油耐受性提高或萜类产量提高的重组宿主细胞、其产生方法及其用途 |
| US11702641B2 (en) | 2016-12-27 | 2023-07-18 | Suntory Holdings Limited | Steviol glycoside hexose transferase and gene coding for same |
| JP7330888B2 (ja) | 2017-01-26 | 2023-08-22 | マナス バイオ インコーポレイテッド | テルペノイド産物を微生物生産するための代謝工学 |
| CN110914445B (zh) | 2017-02-03 | 2024-08-27 | 泰莱解决方案美国有限责任公司 | 工程化糖基转移酶和甜菊醇糖苷葡糖基化方法 |
| EP3611265A4 (en) | 2017-04-12 | 2021-09-01 | Suntory Holdings Limited | RHAMNOSIS SYNTHASE DERIVED FROM STEVIA AND GENE |
| WO2018211032A1 (en) | 2017-05-17 | 2018-11-22 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| WO2019006312A1 (en) * | 2017-06-30 | 2019-01-03 | Myriant Corporation | MICROORGANISM HAVING A NUMBER OF STABILIZED COPIES OF A FUNCTIONAL DNA SEQUENCE AND ASSOCIATED METHODS |
| US11365417B2 (en) | 2017-09-12 | 2022-06-21 | Bio Capital Holdings, LLC | Biological devices and methods of use thereof to produce steviol glycosides |
| US20200347425A1 (en) * | 2017-11-20 | 2020-11-05 | Manus Bio, Inc. | Microbial host cells for production of steviol glycosides |
| BR112020009838A2 (pt) | 2017-12-05 | 2020-11-17 | Evolva Sa | produção de glicosídeos de esteviol em hospedeiros recombinantes |
| CN108070602B (zh) * | 2017-12-12 | 2020-11-06 | 山东隆科特酶制剂有限公司 | 一种减少及推迟芽孢杆菌芽孢生成的分子改造方法 |
| US11608364B2 (en) | 2018-04-30 | 2023-03-21 | Dsm Ip Assets B.V. | Steviol glycoside transport |
| KR101929158B1 (ko) * | 2018-06-07 | 2018-12-13 | 씨제이제일제당 (주) | 5'-크산틸산을 생산하는 미생물 및 이를 이용한 5'-크산틸산의 제조방법 |
| JP7584401B2 (ja) | 2018-07-30 | 2024-11-15 | テイト アンド ライル ソリューションズ ユー・エス・エー エル・エル・シー | 操作されたグリコシルトランスフェラーゼおよびステビオールグリコシドのグルコシル化方法 |
| CN113302298B (zh) | 2018-11-09 | 2025-02-25 | 银杏生物制品公司 | 罗汉果甙的生物合成 |
| SG11202107656TA (en) * | 2019-01-24 | 2021-08-30 | Amyris Inc | Abc transporters for the high efficiency production of rebaudiosides |
| SG11202111698SA (en) | 2019-05-03 | 2021-11-29 | Amyris Inc | Kaurenoic acid 13-hydroxylase (kah) variants and uses thereof |
| CN112322648A (zh) * | 2019-07-30 | 2021-02-05 | 上海市农业科学院 | 一种abc转运蛋白基因mrp1s及其制备方法和应用 |
| US20240148037A1 (en) * | 2019-10-16 | 2024-05-09 | Sweegen, Inc. | Steviol glycoside formulations for food and beverages |
| CN110846363B (zh) * | 2019-11-11 | 2023-02-17 | 天津大学 | 一锅法生产莱鲍迪苷d的方法 |
| EP4143213A1 (en) * | 2020-04-27 | 2023-03-08 | Université Libre de Bruxelles | Yeast strains for reducing contamination by lactic acid bacteria |
| CN114214378A (zh) * | 2021-11-23 | 2022-03-22 | 安徽金禾实业股份有限公司 | 一种利用枯草芽孢杆菌发酵催化制备莱鲍迪苷m的方法 |
| JP2025501616A (ja) * | 2021-12-24 | 2025-01-22 | ザ リージェンツ オブ ザ ユニヴァーシティ オブ カリフォルニア | 酵母におけるサポニンの製造 |
| CN114561310B (zh) * | 2022-03-17 | 2022-12-02 | 江南大学 | 一种生产甜茶苷的酿酒酵母及其应用 |
| CN119372219B (zh) * | 2024-12-11 | 2026-01-02 | 华中农业大学 | 香菇ABC转运蛋白基因LeABC2及其在调控香菇耐热性中的应用 |
| CN119639706A (zh) * | 2024-12-24 | 2025-03-18 | 铭诚惠众(江苏)药物研究有限公司 | 一种糖基转移酶srUGT76G1及其在Reb M的生产中的应用 |
Family Cites Families (142)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58149697A (ja) | 1982-02-27 | 1983-09-06 | Dainippon Ink & Chem Inc | β−１，３グリコシルステビオシドの製造方法 |
| JPS591408A (ja) | 1982-06-28 | 1984-01-06 | Fukue Ito | ヘヤ−トニツクの製造方法 |
| JPS59101408A (ja) | 1982-12-02 | 1984-06-12 | Junichi Iwamura | 植物生長調整剤 |
| US6946587B1 (en) | 1990-01-22 | 2005-09-20 | Dekalb Genetics Corporation | Method for preparing fertile transgenic corn plants |
| US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
| JPH03277275A (ja) | 1990-03-28 | 1991-12-09 | Dainippon Ink & Chem Inc | 新規酵素及びその酵素を用いた配糖体の製造方法 |
| US5204253A (en) | 1990-05-29 | 1993-04-20 | E. I. Du Pont De Nemours And Company | Method and apparatus for introducing biological substances into living cells |
| US5306862A (en) | 1990-10-12 | 1994-04-26 | Amoco Corporation | Method and composition for increasing sterol accumulation in higher plants |
| US5460949A (en) | 1990-11-15 | 1995-10-24 | Amoco Corporation | Method and composition for increasing the accumulation of squalene and specific sterols in yeast |
| US5712112A (en) | 1992-11-04 | 1998-01-27 | National Science Council Of R.O.C. | Gene expression system comprising the promoter region of the alpha-amylase genes |
| FI960173L (fi) | 1993-07-15 | 1996-03-15 | Neose Pharm Inc | Menetelmä sakkaridikoostumusten syntetisoimiseksi |
| US7186891B1 (en) | 1996-04-12 | 2007-03-06 | University Of Kentucky, Research Foundation | Plant cells and plants expressing chimeric isoprenoid synthases |
| JPH10117776A (ja) | 1996-10-22 | 1998-05-12 | Japan Tobacco Inc | インディカイネの形質転換方法 |
| EP1021551A1 (en) | 1997-10-06 | 2000-07-26 | Centre National De La Recherche Scientifique | Plant fatty acid hydroxylase genes |
| US6255557B1 (en) | 1998-03-31 | 2001-07-03 | Her Majesty The Queen In Right Of Canada As Represented By The Ministerof Agriculture And Agri-Food Canada | Stevia rebaudiana with altered steviol glycoside composition |
| CA2325798C (en) | 1998-04-14 | 2008-08-26 | Kyowa Hakko Kogyo Co., Ltd. | A process for producing isoprenoid compounds by microorganisms and a method for screening compounds with antibiotic or weeding activity |
| TWI250210B (en) | 1998-05-06 | 2006-03-01 | Dsm Ip Assets Bv | An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate |
| EP0955363A3 (en) | 1998-05-06 | 2004-01-28 | F. Hoffmann-La Roche Ag | Dna sequences encoding enzymes involved in production of isoprenoids |
| MX219130B (es) | 1998-07-06 | 2004-02-10 | Dcv Inc Haciendo Negocios Como | Metodo para producir vitamina. |
| US6531303B1 (en) | 1998-07-06 | 2003-03-11 | Arkion Life Sciences Llc | Method of producing geranylgeraniol |
| AR021636A1 (es) | 1998-12-17 | 2002-07-31 | Rubicon Forests Holdings Ltd | Materiales y metodos para la modificacion del contenido, la composicion y el metabolismo de los isoprenoides |
| CA2352488A1 (en) | 1998-12-23 | 2000-06-29 | The Samuel Roberts Noble Foundation, Inc. | Plant transformation process |
| DE60034224T2 (de) | 1999-04-15 | 2007-12-20 | Calgene Llc, Davis | Nukleinsäuresequenzen für proteine die an der isoprenoid-synthese beteiligt sind |
| EP1171618A2 (en) | 1999-04-21 | 2002-01-16 | The Samuel Roberts Noble Foundation | Plant transformation process |
| DE60036477T2 (de) | 1999-08-04 | 2008-06-12 | Bacher, Adelbert, Prof. Dr.med. Dr.rer.nat. | Isoprenoid biosynthese |
| AU6780700A (en) | 1999-08-18 | 2001-03-13 | Paradigm Genetics, Inc. | Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration |
| AU2001269685A1 (en) | 2000-05-03 | 2001-11-12 | The Salk Institute For Biological Studies | Crystallization of 4-diphosphocytidyl-2-c-methylerythritol synthesis |
| DE10027821A1 (de) | 2000-06-05 | 2001-12-06 | Adelbert Bacher | Der Mevalonat-unabhängige Isoprenoidbiosyntheseweg |
| EP2292776A1 (en) | 2000-07-31 | 2011-03-09 | Danisco US Inc. | Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms |
| CA2420555C (en) | 2000-08-24 | 2012-10-23 | Jeffrey F. Harper | Stress-regulated genes of plants, transgenic plants containing same, and methods of use |
| US6660507B2 (en) | 2000-09-01 | 2003-12-09 | E. I. Du Pont De Nemours And Company | Genes involved in isoprenoid compound production |
| US6818424B2 (en) | 2000-09-01 | 2004-11-16 | E. I. Du Pont De Nemours And Company | Production of cyclic terpenoids |
| US6689601B2 (en) | 2000-09-01 | 2004-02-10 | E. I. Du Pont De Nemours And Company | High growth methanotropic bacterial strain |
| US7229784B2 (en) * | 2000-09-19 | 2007-06-12 | Microbia, Inc. | Modulation of secondary metabolite production by zinc binuclear cluster proteins |
| US20030219798A1 (en) | 2000-09-29 | 2003-11-27 | Gokarn Ravi R. | Isoprenoid production |
| US6949362B2 (en) | 2000-12-12 | 2005-09-27 | E. I. Du Pont De Nemours And Company | Rhodococcus cloning and expression vectors |
| EP1356037B1 (en) | 2001-01-25 | 2011-03-09 | Evolva Ltd. | A library of a collection of cells |
| US20040078846A1 (en) | 2002-01-25 | 2004-04-22 | Desouza Mervyn L. | Carotenoid biosynthesis |
| US20050003474A1 (en) | 2001-01-26 | 2005-01-06 | Desouza Mervyn L. | Carotenoid biosynthesis |
| DE10201458A1 (de) | 2001-04-11 | 2002-10-17 | Adelbert Bacher | Intermediate und Enzyme des Mevalonat-unabhängigen Isoprenoidbiosyntheseweg |
| US7034140B2 (en) | 2001-04-24 | 2006-04-25 | E.I. Du Pont De Nemours And Company | Genes involved in isoprenoid compound production |
| ATE405638T1 (de) | 2001-06-06 | 2008-09-15 | Dsm Ip Assets Bv | Verbesserte isoprenoid herstellung |
| WO2003008540A2 (en) | 2001-06-22 | 2003-01-30 | Syngenta Participations Ag | Abiotic stress responsive polynucleotides and polypeptides |
| NZ513755A (en) * | 2001-08-24 | 2001-09-28 | Ann Rachel Holmes | Protein expression system in yeast comprising a vector encoding a heterologous membrane protein and its application in screening for drugs |
| US20040072311A1 (en) | 2001-08-28 | 2004-04-15 | Dicosimo Deana J. | Production of cyclic terpenoids |
| US20040010815A1 (en) | 2001-09-26 | 2004-01-15 | Lange B. Markus | Identification and characterization of plant genes |
| US7172886B2 (en) | 2001-12-06 | 2007-02-06 | The Regents Of The University Of California | Biosynthesis of isopentenyl pyrophosphate |
| DE60329505D1 (de) | 2002-08-20 | 2009-11-12 | Suntory Holdings Ltd | Neue glycosyltransferase-gene |
| AU2003273922A1 (en) | 2002-09-27 | 2004-04-19 | Dsm Ip Assets B.V. | Squalene synthase 8sqs9 gen |
| US7098000B2 (en) | 2003-06-04 | 2006-08-29 | E. I. Du Pont De Nemoure And Company | Method for production of C30-aldehyde carotenoids |
| ES2323175T3 (es) | 2003-06-12 | 2009-07-08 | Dsm Ip Assets B.V. | Mevalonato-quinasas resistentes a la retroalimentacion. |
| US7569389B2 (en) | 2004-09-30 | 2009-08-04 | Ceres, Inc. | Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics |
| MXPA06013502A (es) | 2004-05-21 | 2007-03-01 | Univ California | Metodo para mejorar la produccion de compuestos isoprenoides. |
| CA2559760A1 (en) | 2004-07-02 | 2006-07-06 | Metanomics Gmbh | Process for the production of fine chemicals |
| US20060014264A1 (en) | 2004-07-13 | 2006-01-19 | Stowers Institute For Medical Research | Cre/lox system with lox sites having an extended spacer region |
| WO2006016395A1 (ja) | 2004-08-09 | 2006-02-16 | National University Corporation Tohoku University | Ｕｄｐ−グルクロニル基転移酵素およびその遺伝子 |
| US7572609B2 (en) | 2004-08-19 | 2009-08-11 | Dsm Ip Assets B.V. | Production of isoprenoids |
| US7923552B2 (en) | 2004-10-18 | 2011-04-12 | SGF Holdings, LLC | High yield method of producing pure rebaudioside A |
| WO2006063752A1 (en) | 2004-12-14 | 2006-06-22 | Dsm Ip Assets B.V. | Improved mevalonate kinase |
| EP1871883A1 (en) | 2005-03-02 | 2008-01-02 | Metanomics GmbH | Process for the production of fine chemicals |
| WO2006093289A1 (ja) | 2005-03-04 | 2006-09-08 | National Institute Of Advanced Industrial Science And Technology | Udp-キシロースの製造方法 |
| WO2006096392A2 (en) | 2005-03-04 | 2006-09-14 | Diversa Corporation | Enzymes involved in astaxanthin, carotenoid and isoprenoid biosynthetic pathways, genes encoding them and methods of making and using them |
| US7989677B2 (en) | 2005-08-17 | 2011-08-02 | Nestec S. A. | Nucleic acids and proteins associated with sucrose accumulation in coffee |
| US8293307B2 (en) | 2005-10-11 | 2012-10-23 | Purecircle Sdn Bhd | Process for manufacturing a sweetener and use thereof |
| ES2619949T3 (es) | 2005-11-23 | 2017-06-27 | The Coca-Cola Company | Composiciones edulcorantes naturales de alta potencia con perfil temporal y/o perfil de sabor mejorado, métodos para su formulación y usos |
| KR100784261B1 (ko) | 2006-01-02 | 2007-12-11 | 한국과학기술원 | 탄저균의 포자외막 단백질을 이용한 목적단백질의 미생물표면발현방법 |
| US7927851B2 (en) | 2006-03-21 | 2011-04-19 | Vineland Research And Innovation Centre | Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same |
| CA2644273A1 (en) | 2006-04-05 | 2008-03-27 | Metanomics Gmbh | Process for the production of a fine chemical |
| WO2007136847A2 (en) | 2006-05-19 | 2007-11-29 | The Regents Of The University Of California | Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels |
| EP2035827B1 (en) | 2006-06-19 | 2012-10-17 | Givaudan S.A. | Nucleic acid, polypeptide and its use |
| WO2008008256A2 (en) | 2006-07-07 | 2008-01-17 | The Regents Of The University Of California | Methods for enhancing production of isoprenoid compounds by host cells |
| BRPI0716954B1 (pt) | 2006-09-26 | 2024-04-30 | The Regents Of The University Of California | Microrganismos geneticamente modificados capazes de produzir um isoprenóide |
| US7741119B2 (en) | 2006-09-28 | 2010-06-22 | E. I. Du Pont De Nemours And Company | Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production |
| US7629156B2 (en) | 2006-09-28 | 2009-12-08 | E.I. Du Pont De Nemours And Company | Ethanol production in fermentation of mixed sugars containing xylose |
| CN101200480B (zh) | 2006-12-15 | 2011-03-30 | 成都华高药业有限公司 | 莱鲍迪甙a的提取方法 |
| JP4915917B2 (ja) | 2006-12-22 | 2012-04-11 | 独立行政法人農業・食品産業技術総合研究機構 | ラクト−ｎ−ビオースｉ及びガラクト−ｎ−ビオースの製造方法 |
| JP2008237110A (ja) | 2007-03-27 | 2008-10-09 | Institute Of Physical & Chemical Research | ステビオール合成酵素遺伝子及びステビオールの製造方法 |
| BRPI0811747A2 (pt) | 2007-05-17 | 2014-10-14 | Tetravitae Bioscience Inc | Métodos e composições para a produção de solventes |
| US20080292775A1 (en) | 2007-05-22 | 2008-11-27 | The Coca-Cola Company | Delivery Systems for Natural High-Potency Sweetener Compositions, Methods for Their Formulation, and Uses |
| WO2009005704A1 (en) | 2007-07-03 | 2009-01-08 | The Regents Of The University Of California | Methods of increasing isoprenoid or isoprenoid precursor production |
| JP2009034080A (ja) | 2007-08-03 | 2009-02-19 | Sanei Gen Ffi Inc | 新規糖転移酵素、及びそれを利用した配糖体の製造 |
| US7964232B2 (en) | 2007-09-17 | 2011-06-21 | Pepsico, Inc. | Steviol glycoside isomers |
| CN101939435A (zh) | 2007-09-21 | 2011-01-05 | 巴斯夫植物科学有限公司 | 具有增加的产量的植物 |
| EP2214681B1 (en) | 2007-12-03 | 2021-10-20 | DSM IP Assets B.V. | Novel nutraceutical compositions containing steviol and uses thereof |
| EP2230939B1 (en) | 2007-12-27 | 2020-04-08 | Heartland Consumer Products LLC | Synergistic sweetening compositions |
| CA2715397A1 (en) | 2008-02-20 | 2009-08-27 | Ceres, Inc. | Nucleotide sequences and corresponding polypeptides conferring improved nitrogen use efficiency characteristics in plants |
| TWI475963B (zh) | 2008-02-25 | 2015-03-11 | Coca Cola Co | 甜菊糖苷ａ衍生性產物以及製造彼的方法 |
| EP2285948B1 (en) | 2008-03-03 | 2014-01-08 | Joule Unlimited Technologies, Inc. | Engineered co2 fixing microorganisms producing carbon-based products of interest |
| WO2009140394A1 (en) | 2008-05-13 | 2009-11-19 | Cargill, Incorporated | Separation of rebaudioside a from stevia glycosides using chromatography |
| WO2010021001A2 (en) | 2008-08-19 | 2010-02-25 | Kaushik Ramakrishnan S | Process for preparing sweetener from stevia rebaudiana |
| ES2592377T3 (es) | 2008-10-03 | 2016-11-29 | Morita Kagaku Kogyo Co., Ltd. | Nuevos glucósidos de esteviol |
| CN101720910B (zh) | 2008-10-23 | 2012-07-25 | 大闽食品（漳州）有限公司 | 一种甜菊糖甙的制备方法 |
| US8614085B2 (en) * | 2009-02-27 | 2013-12-24 | Butamax(Tm) Advanced Biofuels Llc | Yeast with increased butanol tolerance involving a multidrug efflux pump gene |
| US20100297722A1 (en) | 2009-05-20 | 2010-11-25 | Board Of Trustees Of Southern Illinois University | Transgenic moss producing terpenoids |
| RU2517602C2 (ru) | 2009-06-08 | 2014-05-27 | Йенневайн Биотехнологи Гмбх | Синтез нмо |
| KR20120027363A (ko) | 2009-06-16 | 2012-03-21 | 이피씨 (베이징) 내추럴 프로덕츠 컴퍼니, 리미티드 | 뒷맛을 감소시키거나 제거하기 위한 레바우디오사이드 ｄ를 포함하는 조성물 및 그의 제조 방법 |
| MX2012002749A (es) | 2009-09-04 | 2012-07-04 | Redpoint Bio Corp | Aumentadores de dulzura incluyendo rebaudiosido a o d. |
| MX2012003489A (es) | 2009-09-22 | 2012-07-30 | Redpoint Bio Corp | Polimorfos novedosos de rebaudiosida c y metodos para elaborarlos y utilizarlos. |
| MX2012003686A (es) | 2009-10-15 | 2012-07-25 | Purecircle Sdn Bhd | Rebaudiosido d de alta pureza y aplicaciones. |
| US8299224B2 (en) | 2009-10-15 | 2012-10-30 | Purecircle Sdn Bhd | High-purity Rebaudioside D |
| US8703224B2 (en) | 2009-11-04 | 2014-04-22 | Pepsico, Inc. | Method to improve water solubility of Rebaudioside D |
| MY162232A (en) | 2009-11-10 | 2017-05-31 | Massachusetts Inst Technology | Microbial engineering for the production of chemical and pharmaceutical products from the isoprenoid pathway |
| US8993840B2 (en) | 2009-11-23 | 2015-03-31 | E I du Pont de Nemours and Compay | Sucrose transporter genes for increasing plant seed lipids |
| HUE041436T2 (hu) | 2009-12-10 | 2019-05-28 | Univ Minnesota | Tal-effektor-közvetített DNS-módosítás |
| JP2013515492A (ja) | 2009-12-28 | 2013-05-09 | ザ コカ・コーラ カンパニー | 甘味増強剤、甘味増強剤を含む組成物、及びそれらの使用方法 |
| KR101244315B1 (ko) | 2010-10-19 | 2013-03-14 | 이화여자대학교 산학협력단 | 에탄올―저항성 효모 유전자 및 이의 용도 |
| WO2011140329A1 (en) | 2010-05-06 | 2011-11-10 | Ceres, Inc. | Transgenic plants having increased biomass |
| WO2011146833A1 (en) | 2010-05-20 | 2011-11-24 | Evolva Inc. | Method of producing isoprenoid compounds in yeast |
| WO2011153144A1 (en) | 2010-05-31 | 2011-12-08 | Gevo, Inc. | Isobutanol production using yeasts with modified transporter expression |
| NZ604915A (en) | 2010-06-02 | 2014-10-31 | Evolva Nutrition Inc | Recombinant production of steviol glycosides |
| US20120021111A1 (en) | 2010-07-23 | 2012-01-26 | Aexelon Therapeutics, Inc. | Natural Low Caloric Sweetener Compositions for Use in Beverages, Foods and Pharmaceuticals, and Their Methods of Manufacture |
| US20120050021A1 (en) | 2010-08-27 | 2012-03-01 | Ford Global Technologies, Llc | Method and Apparatus for In-Vehicle Presence Detection and Driver Alerting |
| US20120083593A1 (en) | 2010-10-01 | 2012-04-05 | Shanghai Yongyou Bioscience Inc. | Separation and Purification of Stevioside and Rebaudioside A |
| WO2012075030A1 (en) | 2010-11-30 | 2012-06-07 | Massachusetts Institute Of Technology | Microbial production of natural sweeteners, diterpenoid steviol glycosides |
| KR20130014227A (ko) | 2011-07-29 | 2013-02-07 | 한국생명공학연구원 | 신규한 α-글루코실 스테비오사이드 및 이의 제조 방법 |
| US20140248668A1 (en) | 2011-08-08 | 2014-09-04 | Evolva Sa | Methods and Materials for Recombinant Production of Saffron Compounds |
| SG10201606567TA (en) | 2011-08-08 | 2016-10-28 | Evolva Sa | Recombinant production of steviol glycosides |
| IN2014CN03557A (cg-RX-API-DMAC7.html) | 2011-11-23 | 2015-10-09 | Evolva Sa | |
| CN103159808B (zh) | 2011-12-09 | 2017-03-29 | 上海泓博智源医药股份有限公司 | 一种制备天然甜味剂的工艺方法 |
| EP3345490B1 (en) | 2011-12-19 | 2023-11-08 | The Coca-Cola Company | Beverage comprising rebaudioside x |
| DK2806754T3 (en) | 2012-01-23 | 2019-02-18 | Dsm Ip Assets Bv | Diterpene PREPARATION |
| US10000783B2 (en) | 2012-03-16 | 2018-06-19 | Suntory Holdings Limited | Steviol glucosyltransferases and genes encoding the same |
| CN110358795B (zh) | 2012-05-22 | 2024-12-03 | 谱赛科有限责任公司 | 高纯度的甜菊醇糖苷 |
| WO2014048392A1 (zh) | 2012-09-29 | 2014-04-03 | 中国科学院上海生命科学研究院 | 利用微生物生产甜菊糖苷类化合物的方法 |
| EP2928321A1 (en) | 2012-12-05 | 2015-10-14 | Evolva SA | Steviol glycoside compositions sensory properties |
| CN105051195B (zh) | 2013-02-06 | 2019-09-06 | 埃沃尔瓦公司 | 用于提高莱鲍迪苷d和莱鲍迪苷m之产生的方法 |
| AU2014273054A1 (en) | 2013-05-31 | 2015-12-03 | Dsm Ip Assets B.V. | Extracellular diterpene production |
| WO2014191581A2 (en) | 2013-05-31 | 2014-12-04 | Dsm Ip Assets B.V. | Microorganisms for diterpene production |
| KR101559478B1 (ko) | 2013-06-24 | 2015-10-13 | 한국생명공학연구원 | 효소전환법을 이용한 천연 고감미료의 제조방법 |
| MX2016000452A (es) | 2013-07-15 | 2016-05-12 | Dsm Ip Assets Bv | Produccion de diterpeno. |
| WO2015011209A1 (en) | 2013-07-23 | 2015-01-29 | Dsm Ip Assets B.V. | Diterpene production in yarrowia |
| CN105658081A (zh) | 2013-07-31 | 2016-06-08 | 帝斯曼知识产权资产管理有限公司 | 甜菊糖苷 |
| MX369591B (es) | 2013-07-31 | 2019-11-13 | Dsm Ip Assets Bv | Recuperacion de glicosidos de esteviol. |
| US9957539B2 (en) | 2013-08-02 | 2018-05-01 | Suntory Holdings Limited | Method for using hexenol glycosyl transferase |
| CN103397064B (zh) | 2013-08-14 | 2015-04-15 | 苏州汉酶生物技术有限公司 | 一种酶法制备瑞鲍迪甙m的方法 |
| EP3039132A2 (en) | 2013-08-30 | 2016-07-06 | Evolva SA | A method for producing modified resveratrol |
| EP3114210A2 (en) | 2014-03-07 | 2017-01-11 | Evolva SA | Methods for recombinant production of saffron compounds |
| BR112017002783A2 (pt) | 2014-08-11 | 2017-12-19 | Evolva Sa | produção de glicosídeos de esteviol em hospedeiros recombinantes |
| MY191735A (en) | 2014-09-09 | 2022-07-13 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| JP2018503388A (ja) | 2015-01-30 | 2018-02-08 | エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． | 組み換え宿主におけるステビオールグリコシドの生産 |
| CN104845990A (zh) | 2015-06-11 | 2015-08-19 | 山东大学 | 拟南芥糖基转移酶基因ugt73c7在提高植物抗病性中的应用 |
| AU2016307066A1 (en) | 2015-08-07 | 2018-02-08 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
-
2014
- 2014-02-11 CN CN201480008381.6A patent/CN105189771B/zh active Active
- 2014-02-11 AU AU2014213918A patent/AU2014213918B2/en active Active
- 2014-02-11 JP JP2015556536A patent/JP6576247B2/ja not_active Expired - Fee Related
- 2014-02-11 US US14/764,898 patent/US10017804B2/en active Active
- 2014-02-11 SG SG10201705993YA patent/SG10201705993YA/en unknown
- 2014-02-11 MY MYPI2015001996A patent/MY184253A/en unknown
- 2014-02-11 BR BR112015019160A patent/BR112015019160A2/pt not_active Application Discontinuation
- 2014-02-11 WO PCT/EP2014/052675 patent/WO2014122328A1/en not_active Ceased
- 2014-02-11 KR KR1020157024717A patent/KR20150128705A/ko not_active Withdrawn
- 2014-02-11 SG SG11201506127WA patent/SG11201506127WA/en unknown
- 2014-02-11 EP EP14704558.7A patent/EP2954061B1/en active Active
- 2014-02-11 MX MX2015010098A patent/MX2015010098A/es unknown
- 2014-02-11 CA CA2900882A patent/CA2900882A1/en not_active Abandoned
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