EP0329822A2 - Procédé d'amplification d'acides nucléiques - Google Patents

Procédé d'amplification d'acides nucléiques Download PDF

Info

Publication number
EP0329822A2
EP0329822A2 EP88113948A EP88113948A EP0329822A2 EP 0329822 A2 EP0329822 A2 EP 0329822A2 EP 88113948 A EP88113948 A EP 88113948A EP 88113948 A EP88113948 A EP 88113948A EP 0329822 A2 EP0329822 A2 EP 0329822A2
Authority
EP
European Patent Office
Prior art keywords
sequence
primer
dna
template
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP88113948A
Other languages
German (de)
English (en)
Other versions
EP0329822B1 (fr
EP0329822A3 (en
Inventor
Cheryl Davey
Lawrence T. Malek
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cangene Corp
Original Assignee
Cangene Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=4137506&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP0329822(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cangene Corp filed Critical Cangene Corp
Priority to ZA896476A priority Critical patent/ZA896476B/xx
Publication of EP0329822A2 publication Critical patent/EP0329822A2/fr
Publication of EP0329822A3 publication Critical patent/EP0329822A3/en
Application granted granted Critical
Publication of EP0329822B1 publication Critical patent/EP0329822B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Definitions

  • This invention relates to a process for amplifying a specific nucleic acid sequence.
  • the phrase "specific nucleic acid sequence” means a single stranded or double stranded nucleic acid which one wishes to amplify; "sample” means a mixture containing nucleic acids; "sufficiently complementary” means that two nucleic acids, a primer and a template, are capable of specific interaction which allows efficient, primer-dependent and template-directed synthesis of DNA, under given conditions of ionic strength and temperature.
  • nucleic acid probes are highly specific, it is preferable in some situations to probe the nucleic acid sequence itself rather than the protein produced by the nucleic acid sequence.
  • a diagnostic method based solely on protein detection would be unreliable for determining the presence of infectious particles of hepatitis B virus, due to the presence of significant levels of non-infectious antigen particles which lack the DNA genome.
  • the various subtypes of human papilloma virus found in either pre-cancerous or benign cervical tumors can be distinguished only by the use of nucleic acid probe hybridization.
  • the microbiology of AIDS makes it certain that an assay based on the presence of AIDS specific nucleic acid sequence would be superior as a diagnostic.
  • One method to amplify is to 'grow out' the sample, that is, to arrange conditions so that the living biological material present in the sample can replicate itself. Replication increases the quantity of nucleic acid sequences to detectable levels.
  • food samples In the food industry, for example, in order to test processed food for the food-poisoning bacteria Salmonella, food samples must be incubated for a number of days to increase the quantity of nucleic acids. In clinical samples, pathogens must also be allowed to increase their number by growing out over some considerable time.
  • United States Patent No. 4,683,195 issued on July 28, 1987 to Cetus Corporation and United States Patent No. 4,683,202 issued on July 28, 1987 to Cetus Corporation are each directed to a process for amplifying a target nucleic acid sequence contained in a sample.
  • United States Patent No. 4,683,195 relates to a process in which a sample suspected of containing a target nucleic acid sequence is treated with oligonucleotide primers such that a primer extension product is synthesized which in turn serves as a template, resulting in amplification of the target nucleic acid sequence.
  • the primer extension product is separated from the template in the preferred embodiment using heat denaturation.
  • 4,683,202 relates to a process for amplifying a target nucleic acid sequence having two separate complementary strands.
  • the process includes treating the strands with primers to synthesize extension products, separating the primer extension products from the templates, and in turn using the primer extension products as templates.
  • each cycle of the amplification process takes place by the synthesis from a first template, of a second template, the second template in turn is used to synthesize the first template. This procedure is repeated, thus, each cycle of the amplification process is based on the synthesis of one product from one substrate.
  • This invention relates to an amplification process which is expedient and requires less participation and fewer manipulations by the user of the process than conventional amplification processes.
  • the amplification takes place at a relatively constant ambient temperature.
  • each cycle of the process generates a plurality of copies of product from one substrate.
  • the amplification process of this invention may be used to increase the quantity of a specific nucleic acid thus circumventing the copy number problem. Hence, probe assays may be more readily used.
  • the amplification process could also be used to increase the purity of a specific nucleic acid sequence as a substitute for conventional cloning methodology.
  • a process for amplifying a specific nucleic acid sequence is used.
  • the process involves:
  • a sequence of the first or the second primer is sufficiently complementary to a sequence of the specific nucleic acid sequence and a sequence of the first or the second primer is sufficiently homologous to a sequence of the specific nucleic acid sequence.
  • a 3′ end of the first primer is oriented towards a 3′ end of the second primer on complementary strands.
  • the second primer of DNA has a sequence at its 3′ end which is sufficiently complementary to the DNA sequence of the second template.
  • the second primer has at its 5′ end an antisense sequence of a promoter and an antisense sequence of a transcription initiation site for a RNA polymerase.
  • the third DNA sequence covalently attached to the second template is complementary to the DNA sequence at the 5′ end of the second primer.
  • a process for amplifying a specific nucleic acid sequence involves combining a first primer, a second primer, ribonuclease H, a RNA-directed DNA polymerase, a DNA-directed DNA polymerase, a RNA polymerase, ribonucleoside triphosphates and deoxyribonucleoside triphosphates with a sample.
  • the first primer of DNA has a sequence which is sufficiently complementary to a first template of RNA.
  • the second primer of DNA has a sequence which is sufficiently complementary to a second template of DNA, and an antisense sequence of a promoter and an antisense sequence of a transcription initiation site which are recognized as substrate by the RNA polymerase.
  • a sequence of the first primer or the second primer is sufficiently complementary to a sequence of the specific nucleic acid sequence and a sequence of the first primer or the second primer is sufficiently homologous to a sequence of the specific nucleic acid.
  • a 3′ end of the first primer is oriented towards a 3′ end of the second primer on complementary strands.
  • a process for amplifying a specific nucleic acid sequence involves adding a first primer, a second primer, avian myoblastosis viral polymerase, E. coli ribonuclease H, bacteriophage T7 RNA polymerase, ribonucleoside triphosphates and deoxyribonucleoside triphosphates to a sample.
  • the first primer of DNA has a sequence which is sufficiently complementary to a first template of RNA.
  • the second primer of DNA has a sequence which is sufficiently complementary to a second template of DNA, and an antisense sequence of a promoter and an antisense sequence of a transcription initiation site which are recognized as substrate by T7 RNA polymerase.
  • a sequence of the first primer or the second primer is sufficiently complementary to a sequence of the specific nucleic acid sequence and a sequence of the first primer or the second primer is sufficiently homologous to a sequence of the specific nucleic acid sequence.
  • a 3′ end of the first primer is oriented towards a 3′ end of the second primer on complementary strands.
  • This invention relates to a process for amplifying a specific nucleic acid sequence.
  • the amplification involves the alternate synthesis of DNA and RNA and is generally illustrated in Figure 1.
  • single-stranded RNA is converted to single-stranded DNA which in turn is converted to a functional template for the synthesis of a plurality of copies of the original single-stranded RNA.
  • a first primer and a second primer are used in the amplification process.
  • a sequence of the first primer or the second primer is sufficiently complementary to a sequence of the specific nucleic acid sequence and a sequence of the first or the second primer is sufficiently homologous to a sequence of the specific nucleic acid sequence.
  • both the first primer and second primer are sufficiently complementary and sufficiently homologous to a sequence of the specific nucleic acid sequence, for example, if the specific nucleic acid sequence is double stranded DNA.
  • the RNA is converted to single-stranded DNA by hybridizing an oligonucleotide primer (the first primer) to the RNA (the first template) and synthesizing a complementary strand of DNA from the first primer, (the first DNA sequence) by using a RNA-directed DNA polymerase.
  • the resulting single-stranded DNA (the second template) is separated from the first template by, for example, hydrolysis of the first template and by using a ribonuclease which is specific for RNA-DNA hybrids (for example, ribonuclease H).
  • the second template is converted to a form which is capable of RNA synthesis by hybridizing a synthetic oligonucleotide (the second primer), which contains at its 3′ end a sequence which is sufficiently complementary to the 3′ end of the second template and toward its 5′ end a sequence containing the antisense strand of a promoter and antisense sequence of a transcription initiation site, and by synthesizing a second DNA sequence covalently attached to the 3′ end of the second primer using the second template as a template and synthesizing a third DNA sequence covalently attached to the 3′ end of the second template using the second primer as a template, using DNA-directed DNA polymerase.
  • the second primer synthetic oligonucleotide
  • the resulting functional derivative of the second template which is a third template, is used for the synthesis of a plurality of copies of RNA, the first template, by using a RNA polymerase which is specific for the promoter and transcription initiation site defined by the second primer.
  • Each newly synthesized first template can be converted to further copies of the second template and the third template by repeating the cycle. In addition, repetition of the cycle does not require participation or manipulation by the user.
  • the amplification process commences with the addition of a suitable template nucleic acid to the appropriate enzymes, primers, and cofactors under the appropriate reaction conditions.
  • This template nucleic acid is in a form which is capable of homogenous and continuous amplification and can function as an intermediate in the cycle set forth in Figure 1.
  • the amplification process involves the net consumption of precursors (primers, ribonucleoside triphosphates and deoxyribonucleoside triphosphates) and the net accumulation of products (RNA and DNA).
  • precursors primary, ribonucleoside triphosphates and deoxyribonucleoside triphosphates
  • RNA and DNA the net accumulation of products
  • the processes of RNA and DNA synthesis will proceed asynchronously until sufficient levels of nucleic acids have been synthesized to allow detection.
  • the amplification process may be monitored by, for example, the synthesis of a labeled product from a labeled precursor.
  • the first primer is an oligodeoxyribonucleotide which has at its 3′ end a sequence which is sufficiently complementary to the 3′ end of the first template.
  • the sequence at the 3′ end of the first primer has a particular length and base composition to allow specific and efficient synthesis of the first DNA sequence, under the given conditions of ionic strength and temperature.
  • the first primer may be sufficiently complementary to a region internal to the 3′ end of the first template in the first cycle. In subsequent cycles, the 5′ end of the first primer would be complementary to the 3′ end of the first template. It is contemplated that the first primer may be composed partially or completely of nucleotides or nucleotide analogs other than the natural deoxyribonucleotides.
  • the 5′ end of the first primer may contain sequences which are not complementary to the first template in the first cycle.
  • the non-complementary sequences may be complementary to a nucleic acid which can be immobilized, or to which can be bound a useful non-nucleic acid component, such as a reporter to facilitate detection.
  • the non-complementary sequences may include an antisense sequence of a promoter and an antisense sequence of a transcription initiation site, which could be used for the synthesis of RNA. This RNA would be complementary to the first template and could be used as an intermediate in another amplification cycle.
  • the second primer is an oligodeoxyribonucleotide which contains at its 3′ end a sequence which is sufficiently complementary to the 3′ end of the second template.
  • the second primer has a particular length and base composition to allow specific and efficient synthesis of the second and third DNA sequences, under the given conditions of ionic strength and temperature.
  • the second primer contains the antisense sequence of a functional promoter and the antisense sequence of a transcription initiation site. This sequence, when used as a template for synthesis of the third DNA sequence, contains sufficient information to allow specific and efficient binding of a RNA polymerase and initiation of transcription at the desired site.
  • the promoter sequence may be derived from the antisense strand of a functional promoter.
  • the transcription initiation site may be derived from the 5′ terminal sequence of a natural RNA transcript.
  • the 5′-terminal sequence of the second primer is AATTCTAATACGACTCACTATAGGGAG. This sequence contains the antisense sequence of the promoter and the antisense sequence of the transcription initiation site for T7 RNA polymerase.
  • the transcription initiation site and promoter for another phage RNA polymerase may be used.
  • sequences which are unrelated to the promoter function may be included at the 5′ end of the second primer or between the transcription initiation site and the sequence at the 3′ end which hybridizes to the second template.
  • the second primer may be composed partially or completely of nucleotides or nucleotide analogs other than natural deoxyribonucleotides.
  • Each enzyme or enzyme preparation should be free of deleterious deoxyribonuclease (“DNase”) activities, such as the 5′ or 3′ exonuclease activities which are often associated with certain DNA polymerases and single-strand or double-strand specific exonuclease or endonucleases.
  • DNase deoxyribonuclease
  • Each enzyme or enzyme preparation should be free of deleterious ribonuclease (“RNase”) activities, with the exception of the preferred addition of a ribonuclease activity which is specific for hybrids of RNA and DNA (for example, ribonuclease H).
  • RNase deleterious ribonuclease
  • each enzyme should be reasonably active under the common reaction conditions which are used for the other enzymatic processes, and non-enzymatic processes, such as hybridizing oligonucleotide primers to the RNA or DNA templates.
  • RNA-directed RNA polymerase may be used. It should be understood that if alternative RNA polymerases are used, then the necessary changes to the promoter and initiation sequences of the second primer should be made according to the template specificity of the particular RNA polymerase.
  • RNA-directed DNA polymerase which is used in this invention may be any enzyme capable of synthesizing DNA from an oligodeoxyribonucleotide primer and a RNA template.
  • this enzyme may contain activities for DNA-directed DNA polymerase and RNase H.
  • the avian myoblastosis viral polymerase (“AMV reverse transcriptase") is used.
  • the RNA-directed DNA polymerase could be from another retrovirus, such as Maloney murine leukemia virus.
  • RNA-directed DNA polymerases could be used.
  • the DNA-directed DNA polymerase which is used in this invention may be any enzyme capable of synthesizing DNA from an oligodeoxyribonucleotide primer and a DNA template. This enzyme should not contain either 5′- or 3′- exonuclease activities, which are associated with many types of DNA polymerase. In the preferred embodiment, the AMV reverse transcriptase is used. However, other DNA-directed DNA polymerases which naturally lack the 5′- or 3′- exonuclease activities could be used. These could include certain eukaryotic DNA polymerases, such as, DNA polymerase ⁇ or ⁇ those DNA polymerases which could be isolated from a mammalian tissue, such as calf thymus.
  • DNA polymerase could be made useful by removing the undesirable exonuclease activities either by alteration of the DNA polymerase gene followed by expression of the altered polymerase in a suitable host cell, or by chemical modification of the DNA polymerase protein.
  • Altered versions of DNA polymerase could be made from the Klenow fragment of E. coli DNA polymerase I or the bacteriophage T7 DNA polymerase. It should be understood that such alternative DNA-directed DNA polymerase activities are added to supplement the activity contributed by the RNA-directed DNA polymerase, since in the preferred embodiment, both RNA-directed and DNA-directed DNA polymerase activities are supplied by the same enzyme.
  • the RNase H which could be used in this invention may be any enzyme capable of hydrolyzing a RNA which is annealed to a complementary DNA. This enzyme should not be capable of hydrolyzing single or double-stranded RNA or any DNA.
  • the E. coli RNase H is used.
  • other RNase H enzymes could be used, such as calf thymus RNase H. Since RNase H is an intrinsic activity of AMV reverse transcriptase, the E. coli RNase H will be supplemented in the preferred embodiment by the RNase H of AMV reverse transcriptase.
  • any other enzyme capable of separating the second template from the first template could be used.
  • the abovementioned enzymes and primers are mixed together in a reaction vessel which contains the necessary buffers and cofactors for both DNA and RNA synthesis.
  • the ionic conditions and reaction temperature should be compatible with specific hybridization of the primers to the DNA and RNA templates as is known to those skilled in the art.
  • the reaction mixture should be free of such agents which would interfere with the amplification process, specifically substances which could greatly inhibit the activity of the enzymes, interfere with the hybridizing of primers and templates, or degrade non-productively the nucleic acid intermediates and products.
  • a labeled precursor may be added to the reaction mixture.
  • Amplification is determined by quantitive or qualitative analysis of labeled products, which can be separated from the labeled precursor by using methods known in the art.
  • a labeled precursor may be a ribonucleoside triphosphate for detecting RNA synthesis, or a deoxynucleoside triphosphate or an oligonucleotide primer for detecting DNA synthesis.
  • the type of label may be a radioisotope or a useful chemical group, such as biotin, a chromophore, a fluorophore, or a hapten which could bind to an antibody, or possibly a protein or an enzyme.
  • the labeled products may be separated from the labeled precursors on the basis of solubility, charge, or size.
  • the labeled DNA or RNA may be hybridized to a nucleic acid which contains a complementary sequence and which can be immobilized.
  • the products of the amplification process may be bound to an immobilized support, hybridized to a nucleic acid probe containing a complementary sequence, and separated from the unhybridized nucleic acid probe which remains in solution.
  • the products, DNA or RNA may be bound directly to a solid support by any stable interaction, such as hydrophobic, electrostatic, or covalent interaction.
  • the products may contain certain chemical groups, for example, biotin, which may be incorporated into the products during the amplification process to allow binding to an immobilized protein, for example, avidin or streptavidin.
  • the products may be hybridized to a nucleic acid which contains a complementary sequence and which can be immobilized.
  • the nucleic acid probe would contain a complementary sequence which forms a sufficiently stable interaction with a product of the amplification process to allow binding under the conditions of hybridization and sustained binding under the conditions used for removal of the unhybridized nucleic acid probe.
  • the complementary sequence would be derived from that part of the specific nucleic acid sequence which is between the sequences of the first primer and the second primer.
  • the nucleic acid probe may be a single-stranded DNA or RNA, or a double-stranded DNA or RNA which can be made single-stranded, or an oligonucleotide which can be composed of deoxyribonucleotides and/or ribonucleotides.
  • the nucleic acid probe may contain a chemical group which could covalently bind to a product DNA or RNA under the appropriate conditions.
  • the nucleic acid probe may be labeled with a radioisotope or a useful chemical group, such as biotin, a chromophore, a fluorophore, or a hapten which could bind to an antibody.
  • the nucleic acid probe could be conjugated to a protein or enzyme, for example, a phosphatase or a peroxidase.
  • the nucleic acid probe may contain sequences which would allow in vitro replication of the probe.
  • the products of the amplification process may be analyzed by methods which are typically used for nucleic acids that have been enriched by molecular cloning techniques.
  • the synthesis of a specific DNA sequence may be detected by digestion of the synthesized DNA with a restriction endonuclease, followed by electrophoretic separation and detection using methods known in the art.
  • the sequence of amplified RNA may be determined by DNA synthesis using a RNA-directed DNA polymerase, the first primer, and dideoxynucleoside triphosphates (Stoflet et al., 1988).
  • the sequence of the amplified third template may be determined by RNA synthesis using the DNA-directed RNA polymerase used in the amplification process, and 3′-deoxyribonucleoside triphosphates (Axelrod & Kramer, 1985).
  • the amplified RNA may encode a polypeptide which could be translated, in vitro . The polypeptide product of the in vitro translation could be analyzed by using an antibody.
  • a sample suspected of containing or known to contain the specific nucleic acid sequence is added to the reaction mixture in the form of a template nucleic acid which is capable of homogeneous and continuous amplification and may be any intermediate in the cycle set forth in Figure 1.
  • the template nucleic acid may be a single-stranded RNA which contains at its 5′ end a sequence which is sufficiently homologous to that which is at the 3′ end of the second primer, and contains a sequence which is sufficiently complementary to the first primer.
  • a template nucleic acid of this form would function as a first template in the amplification process.
  • the template nucleic acid may be a single-stranded DNA which contains at its 3′ end a sequence which is sufficiently complementary to at least the 3′ end of the second primer, and contains a sequence which is sufficiently homologous to that which is at the 3′ end of the first primer.
  • a template nucleic acid of this form would function as a second template in the amplification process.
  • the template nucleic acid may be a double-stranded DNA, one strand of which contains at its 5′ end the entire sequence of the second primer and contains a sequence which is sufficiently complementary to the first primer. The double-stranded DNA functions as a third template in the amplification process.
  • a template nucleic acid which could function as a first template could be a naturally occurring RNA or a RNA fragment which could be generated from a larger RNA molecule by using site specific hydrolysis methods known in the art (Shibahara et al., 1987).
  • a template nucleic acid which could function as a second template could be generated from a single-stranded DNA or RNA to which has been hybridized an oligonucleotide which is capable of blocking DNA synthesis.
  • This blocking oligonucleotide may contain a chemical group, which could covalently bind to the template, under the appropriate conditions.
  • DNA synthesis from this blocked template using the first primer could result in a synthesized DNA with the same 3′ end as the second template.
  • the original template is RNA, then the resulting DNA-RNA hybrid may be used directly as a template nucleic acid. If the original template is DNA, then the resulting copy of the second template could then be separated from the original template by using chemical or thermal denaturation methods.
  • This single-stranded DNA could be converted to a transcriptionally functional double-stranded DNA by hybridizing the first primer to the single-stranded DNA, and by synthesizing a DNA sequence which is covalently attached to the first primer and complementary to the single-stranded DNA.
  • Oligonucleotides were synthesized using an Applied Biosystems 380A DNA synthesizer. Columns, phosphoramidites, and reagents used for oligonucleotide synthesis were obtained from Applied Biosystems, Inc. through Technical Marketing Associates. Oligonucleotides were purified by polyacrylamide gel electrophoresis followed by DEAE cellulose chromatography. The radioisotope [ ⁇ -32p] UTP (800 Ci/mmol) was from Amersham. Enzymes for digesting and ligating DNA were purchased from New England Biolabs, and used according to the supplier's recommendations. Preparations containing the large fragment of DNA polymerase 1 (Klenow) were also purchased from New England Biolabs.
  • E. coli transformants were grown on YT medium (Miller, 1972) containing 50 ug/ml ampicillin. Plasmid DNA was purified by a rapid boiling method (Holmes and Quigley, 1981). DNA fragments and vectors used for all constructions were separated by electrophoresis on low melting point agarose, and purified from the molten agarose by phenol extraction and ethanol precipitation (Maniatis et al., 1982). Plasmid DNA was sequenced using a modification (Hattori et al., 1985) of the dideoxy method (Sanger et al., 1977). Reactions were run using the -20 universal primer (New England Biolabs).
  • Example 1 Design and synthesis of oligonucleotides for a gag test system
  • a synthetic DNA sequence ( Figure 2A) was designed to include an EcoRI site, a T7 phage promoter, a sequence required for initiation of transcription by T7 RNA polymerase and a 19 bp hybridization region (hybridization region 1).
  • the 47 b antisense strand oligonucleotide (T7H1.GAG) involved in the cloning of these elements also serves as the first primer.
  • Hybridization region 2 lies 53 bp away from hybridization region 1 and is 20 bp in length.
  • the primer made to this region (H2.GAG) is a 20 b oligonucleotide duplicate of the sense strand and is not used for cloning.
  • gag test DNA could be transcribed by T7 RNA polymerase to produce a RNA fragment (first template) useful as an amplification intermediate involved in three of the steps.
  • T7H1.GAG and H2.GAG serve as primers in the test system.
  • the standard 50 ul reaction mixture used to amplify RNA transcribed from the gag test oligonucleotides contained 0.34 uM T7H1.GAG, 0.34 uM H2.GAG, 50 mM TrisHCl (pH 8.45), 6 mM MgCl2, 40 mM KCl, 10 mM DTT, 0.5 mM NTP, 1 mM dNTP, 40 units RNasin, 20 units T7 RNA polymerase, 20 units reverse transcriptase, 0.8 units RNase H and 10 - 20 uCi [ ⁇ -32p] UTP.
  • the reactions contained amounts of template (N2.GAG) varying from 1 ng to 1 fg. One reaction contained no template.
  • RNA amplification from N2.GAG after 3h Reaction Template RNA Synthesized (ug) Fold amplification 1 1 ng 3.5 3.5 x 103 2 100 pg 4.4 4.4 x 104 3 10 pg 4.1 4.1 x 105 4 1 pg 3.0 3.0 x 106 5 100 fg 2.7 2.7 x 107 6 10 fg 1.9 1.9 x 108 7 1 fg 0.78 7.8 x 108 8 - 0.046 -
  • Example 6 Use of DNA restriction fragment as template.
  • RNA which was synthesized after a reaction time of 3h was analyzed by PAGE ( Figure 6, lanes 1-6, 11 and 12, numbered the same as the reactions).
  • the major band representing a RNA of about 100 b was present in reactions (lanes) 1-6 but absent in the no template reactions (lanes 11 and 12).
  • the RNA in lane 0 was a standard, which was prepared following the teaching of Example 3. There was no apparent qualitative difference in the synthesized RNA either with (lanes 2, 4 and 6) or without (lanes 1, 3, and 5) the additional of 1 ug of MspI-degested calf thymus DNA.
  • Example 8 Use of ribosomal RNA as a template; amplification of internal sequences
  • Example 9 Use of ribosomal RNA as a template; amplification of 5′-terminal sequences.
  • Two primers are used for amplifying RNA sequences which are homologous to a part of E.Coli 16S rRNA.
  • One of these primers RIB12.PR (TTACTCACCCGTCCGCC) is complementary to 16S rRNA.
  • the other T7H1RIB5.PR (AATTCTAATACGACTCACTATAGGGAGAAATTGAAGAGTTTGATCAT) is complementary to the 3′end of the DNA synthesized by using RIB12.PR as a primer and 16S rRNA as a template.
EP88113948A 1988-02-24 1988-08-26 Procédé d'amplification d'acides nucléiques Expired - Lifetime EP0329822B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ZA896476A ZA896476B (en) 1988-08-26 1989-08-24 Nucleic acid amplification process

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA000559709A CA1340807C (fr) 1988-02-24 1988-02-24 Procede d'amplification d'une sequence d'acide nucleique
CA559709 1988-02-24

Publications (3)

Publication Number Publication Date
EP0329822A2 true EP0329822A2 (fr) 1989-08-30
EP0329822A3 EP0329822A3 (en) 1990-08-01
EP0329822B1 EP0329822B1 (fr) 1994-06-08

Family

ID=4137506

Family Applications (1)

Application Number Title Priority Date Filing Date
EP88113948A Expired - Lifetime EP0329822B1 (fr) 1988-02-24 1988-08-26 Procédé d'amplification d'acides nucléiques

Country Status (8)

Country Link
US (1) US5409818A (fr)
EP (1) EP0329822B1 (fr)
KR (2) KR960015744B1 (fr)
AT (1) ATE106948T1 (fr)
CA (1) CA1340807C (fr)
DE (1) DE3850093T2 (fr)
ES (1) ES2053648T3 (fr)
WO (1) WO1991002814A1 (fr)

Cited By (179)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0479376A1 (fr) 1990-10-05 1992-04-08 Akzo Nobel N.V. Peptides agissants immunochimiquemeur avec des anticorps contre le virus d'hépatite non-A, non-B
WO1992008800A1 (fr) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Amplification d'acides nucleiques par replication bi-enzymatique auto-entretenue de sequences
WO1993015228A1 (fr) * 1992-01-29 1993-08-05 Hitachi Chemical Co., Ltd. Support d'immobilisation de polynucleotide
EP0566198A3 (fr) * 1992-04-13 1994-08-03 Enichem Spa
ES2056028A1 (es) * 1993-02-18 1994-09-16 Inia Procedimiento para la seleccion de oligonucleotidos para la amplificacion especifica de genomas altamente variables.
WO1995003430A1 (fr) * 1993-07-23 1995-02-02 Gen-Probe Incorporated Procede d'amelioration de l'amplification de l'acide nucleique
WO1995027721A1 (fr) * 1994-04-07 1995-10-19 Akzo Nobel N.V. Compositions lyophilisees comprenant de l'arn
EP0688873A2 (fr) 1994-06-23 1995-12-27 Bayer Ag Test DNA rapide pour la détection de staphylococcus aureus résistant à la chinolone dans du matériel clinique
WO1996006191A3 (fr) * 1994-08-18 1996-03-14 Akzo Nobel Nv Amorces et sondes destinees a l'amplification et a la detection de l'acide nucleique du cytomegalovirus
EP0710724A2 (fr) 1994-10-06 1996-05-08 Akzo Nobel N.V. Antigènes de Toxoplasma gondii
EP0713922A1 (fr) 1994-10-28 1996-05-29 BIO MERIEUX, Société anonyme Oligonucléotide utilisable comme amorce dans une méthode d'amplification basée sur une réplication avec déplacement de brin
US5554516A (en) * 1992-05-06 1996-09-10 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
EP0778352A1 (fr) * 1988-12-16 1997-06-11 Siska Diagnostics, Inc. Essai par réplication auto-entretenue de séquences
US5643730A (en) * 1991-09-23 1997-07-01 Pfizer Inc. Process for detecting specific mRNA and DNA in cells
WO1998006736A1 (fr) 1996-08-14 1998-02-19 Life Technologies, Inc. Compositions stables pour l'amplification et le sequençage d'acides nucleiques
EP0731174A3 (fr) * 1989-07-11 1998-03-25 Gen-Probe Incorporated Méthodes pour l'amplification de séquences d'acide nucléique
US5766849A (en) * 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
US5789169A (en) * 1994-11-03 1998-08-04 Regents Of The University Of California Methods for the diagnosis of glaucoma
EP0803578A3 (fr) * 1996-04-26 1999-01-13 Toyo Boseki Kabushiki Kaisha Coffret pour l'amplification et la détection des acides nucléiques du cytomegalovirus (CMV) utilisant une séquence d'acide nucléique ss2.7
WO1999046400A1 (fr) 1998-03-13 1999-09-16 Life Technologies, Inc. Compositions et procedes d'augmentation de la synthese des molecules d'acide nucleique
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
US6031150A (en) * 1994-04-13 2000-02-29 Baylor College Of Medicine Sulfonylurea receptor trangenic rodents
US6132954A (en) * 1996-08-20 2000-10-17 Baylor College Of Medicine Methods of screening for agents that delay a cell cycle and compositions comprising era and an analogue of wild-type era
WO2000073764A2 (fr) 1999-06-01 2000-12-07 Baylor College Of Medicine Compositions et methodes pour l'utilisation therapeutique d'une sequence associee au gene atonal dans le traitement de la surdite, de l'osteoarthrite et d'une proliferation cellulaire anormale
US6171788B1 (en) 1997-01-28 2001-01-09 The Regents Of The University Of California Methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
US6225097B1 (en) 1997-09-17 2001-05-01 Toyota Jidosha Kabushiki Kaisha Decaprenyl diphosphate synthetase gene
US6248867B1 (en) 1994-11-03 2001-06-19 Univ California Trabecular meshwork induced glucocorticoid response (TIGR) fusion protein
US6261773B1 (en) 1998-03-17 2001-07-17 Toyo Boseki Kabushiki Kaisha Reagent for nucleic acid amplification and process for nucleic acid amplification
WO2001064959A1 (fr) * 2000-03-02 2001-09-07 Akzo Nobel N.V. Detection d'arn du virus de l'hepatite b
WO2001066705A1 (fr) * 2000-03-07 2001-09-13 Akzo Nobel N.V. Mutants d'arn polymerases presentant une stabilite thermique amelioree
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
EP0776376B1 (fr) * 1994-07-15 2001-11-21 Akzo Nobel N.V. Utilisation d'arn polymerase pour ameliorer un procede d'amplification d'acide nucleique
WO2002004511A2 (fr) 2000-07-10 2002-01-17 Board Of Regents, The University Of Texas System Genes du chromosome 3p21.3 constituant des suppresseurs de tumeurs
EP1229130A2 (fr) * 2000-12-04 2002-08-07 PrimaGen B.V. Tests basés sur les acides nucléiques des organelles cellulaires endosymbiontiques et les composés identifiables
US6475724B1 (en) 1997-01-28 2002-11-05 The Regents Of The University Of California Nucleic acids, kits, and methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
WO2002095002A2 (fr) 2001-05-22 2002-11-28 University Of Chicago Arn polymerase dependant de l'adn a brin unique de virion n4
WO2003053220A2 (fr) 2001-12-17 2003-07-03 Corixa Corporation Compositions et procedes applicables a la therapie et au diagnostic pour la maladie intestinale inflammatoire
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
EP0525882B1 (fr) * 1991-08-02 2004-01-14 bioMerieux B.V. Quantification d'acides nucléiques
WO2004062599A2 (fr) 2003-01-06 2004-07-29 Corixa Corporation Composes d'aminoalkyle glucosaminide phosphate et leur utilisation
US6808883B1 (en) 1999-04-15 2004-10-26 Bayer Ag Automatable rapid test for detection of cancer, based on telomerase (hTC) mRNA with specific primers and probes
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
WO2005019409A2 (fr) 2002-07-15 2005-03-03 Board Of Regents, The University Of Texas System Criblage combinatoire de banques de proteines par expression periplasmique
US6905858B2 (en) 1997-01-02 2005-06-14 Invitrogen Corporation Nucleic acid-free thermostable enzymes and methods of production thereof
WO2005112544A2 (fr) 2004-02-19 2005-12-01 The Governors Of The University Of Alberta Polymorphismes du promoteur de la leptine et utilisations correspondantes
USRE38960E1 (en) * 1994-03-16 2006-01-31 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US7026117B2 (en) 1990-02-16 2006-04-11 Orchid Biosciences, Inc. Method for determining specific nucleotide variations
US7029861B1 (en) 1998-09-15 2006-04-18 Board Of Regents, The University Of Texas System LPS-response gene compositions and methods
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
US7105318B2 (en) 1997-11-04 2006-09-12 Roche Diagnostics Gmbh Specific and sensitive nucleic acid detection method
US7138511B1 (en) 1997-01-28 2006-11-21 The Regents Of The University Of California Nucleic acids, kits and methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
WO2006122354A1 (fr) 2005-05-17 2006-11-23 Ozgene Pty Ltd Systeme de clonage sequentiel
US7141420B2 (en) 1997-02-27 2006-11-28 University Of Utah Research Foundation Nucleic acid and amino acid sequences for ATP-binding cassette transporter and methods of screening for agents that modify ATP-binding cassette transporter
EP1734134A2 (fr) 1999-02-09 2006-12-20 Laboratory Corporation of America Holdings, Inc. Nouveau gène spécifique à la prostate servant au diagnostic, au prognostic et au traitment du cancer de la prostate
WO2007016275A2 (fr) 2005-08-02 2007-02-08 Focus Diagnostics, Inc. Procedes et compositions pour detecter le virus bk
EP1801220A2 (fr) 1997-12-18 2007-06-27 Monsanto Technology, LLC Plantes transgéniques résistant aux insectes et procédés permettant d'améliorer l'activité de delta-toxine contre des insectes
US7306804B2 (en) 1998-11-17 2007-12-11 Board Of Regents, The University Of Texas System HIV-specific T-cell induction
US7342111B2 (en) 1999-04-30 2008-03-11 University Of Florida Research Foundation, Inc. Adeno-associated virus-delivered ribozyme compositions and methods of use
EP1908851A2 (fr) 2001-09-19 2008-04-09 Intergenetics Incorporated Analyse génétique pour la stratification du risque de cancer
WO2008067547A2 (fr) 2006-11-30 2008-06-05 Research Development Foundation Bibliothèques d'immunoglobulines améliorées
WO2008092866A1 (fr) 2007-01-29 2008-08-07 Scientific Institute Of Public Health (Iph) Détection d'événements dans des plantes transgéniques
EP1975231A1 (fr) 2002-01-22 2008-10-01 Corixa Corporation Compositions et procédés de détection, diagnostic et de thérapie de malignancies hématologiques
EP1978098A2 (fr) 1999-12-10 2008-10-08 Invitrogen Corporation Utilisation de sites à recombinaison multiples avec une spécificité unique de clonage recombinatoire
US7449292B2 (en) 2003-09-12 2008-11-11 University Of Cincinnati Methods for predicting relative efficacy of a beta blocker therapy based on a B1-adrenergic receptor polymorphism
WO2008137475A2 (fr) 2007-05-01 2008-11-13 Research Development Foundation Banques d'immunoglobulines fc
EP1997889A2 (fr) 2003-01-29 2008-12-03 454 Corporation Procédé de préparation de bibliothèques ADN à simple brin
US7494788B2 (en) 2005-07-11 2009-02-24 Molecular Kinetics, Inc. Entropic bristle domain sequences and their use in recombinant protein production
US7501252B2 (en) 1992-01-10 2009-03-10 Life Technologies Corporation Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
EP2044948A1 (fr) 2002-08-12 2009-04-08 Jennerex Biotherapeutics ULC Procédés et compositions concernant des poxvirus et le cancer
US7537886B1 (en) 1999-06-22 2009-05-26 Life Technologies Corporation Primers and methods for the detection and discrimination of nucleic acids
US7585512B1 (en) 1990-05-08 2009-09-08 Thomas Jefferson University Composition and method of using tumor cells
EP2105502A1 (fr) 2000-12-12 2009-09-30 Corixa Corporation Composés et procédés de thérapie et de diagnostic du cancer du poumon
EP2105496A1 (fr) 2000-12-08 2009-09-30 Life Technologies Corporation Méthodes et compositions destinées à la synthèse de molécules d'acide nucléique à l'aide de sites de reconnaissance multiples
EP2135960A1 (fr) 2001-09-19 2009-12-23 Intergenetics Incorporated Analyse génétique pour la stratification du risque de cancer par détermination du profile allelique des génes VDR-ApaI et CYP11b2
EP2184289A1 (fr) 1997-04-22 2010-05-12 Life Technologies Corporation Procédés de production de transcriptases inverses ASLV composées de plusieurs subunités
EP2192128A2 (fr) 2000-04-21 2010-06-02 Corixa Corporation Composés et méthodes pour le traitement et le diagnostique des infections de Chlamydia
EP2246444A1 (fr) 2004-09-14 2010-11-03 The Regents of the University of Colorado, A Body Corporate Procédé de traitement faisant intervenir du bucindolol fondé sur le ciblage génétique
EP2270034A2 (fr) 2004-06-03 2011-01-05 Athlomics Pty Ltd Agents et procédé permettant le diagnostic de stress
WO2011008530A2 (fr) 2009-06-29 2011-01-20 Luminex Corporation Amorces chimériques avec conformations en épingle à cheveux et procédés d'utilisation de celles-ci
EP2295551A1 (fr) 2000-05-26 2011-03-16 Life Technologies Corporation Transcriptases inverses thermostables et utilisations
EP2295569A1 (fr) 2003-07-25 2011-03-16 Ambion, Inc. Procédés et compositions pour isoler des petites molécules de RNA
EP2299267A2 (fr) 2005-11-16 2011-03-23 Novartis AG Biomarqueurs pour un traitement par des anticorps anti-NogoA de lésions de la moelle épinière
EP2298891A1 (fr) 2003-07-25 2011-03-23 Ambion, Inc. Procédés et compositions pour isoler des petites molécules d'ARN
EP2319941A2 (fr) 2005-10-21 2011-05-11 GeneNews Inc. Procédé et appareil pour corréler des niveaux de produits biomarqueurs avec une maladie
EP2325303A2 (fr) 2002-09-13 2011-05-25 Life Technologies Corporation Transcriptases inverses thermostables et leurs utilisations
EP2336367A1 (fr) 1997-08-08 2011-06-22 bioMerieux B.V. Séquences nucléotidiques pouvant être utilisées comme amorces et comme sondes au cours de l'amplification et la détection de tous les sous-types du VIH-1
WO2011079273A2 (fr) 2009-12-23 2011-06-30 Arca Biopharma, Inc. Procédés et compositions pour maladies et affections cardiovasculaires
EP2365079A1 (fr) 2005-03-05 2011-09-14 Seegene, Inc. Procédés utilisant un oligonucléotide à double spécificité et oligonucléotide à double spécificité
EP2365078A1 (fr) 2005-03-05 2011-09-14 Seegene, Inc. Procédés utilisant un oligonucléotide à double spécificité et oligonucléotide à double spécificité
EP2366712A2 (fr) 1997-01-30 2011-09-21 The Board Of Regents, The University Of Texas System Suppresseur de tumeur désigné par TS10Q23.3
EP2368868A1 (fr) 2005-10-28 2011-09-28 Praecis Pharmaceuticals Inc. Procédé d'identification de composés intéressants utilisant des bibliothèques codées
WO2011127933A1 (fr) 2010-04-16 2011-10-20 Nuevolution A/S Complexes bifonctionnels et procédés de fabrication et d'utilisation de tels complexes
WO2011133933A2 (fr) 2010-04-23 2011-10-27 University Of Florida Research Foundation, Inc. Compositions de guanylate cyclase raav et méthodes de traitement de l'amaurose-1 congénitale de leber (lca1)
WO2011139721A1 (fr) 2010-04-27 2011-11-10 The Regents Of The University Of California Biomarqueurs de cancer et leurs procédés d'utilisation
WO2011145777A1 (fr) 2010-05-20 2011-11-24 광주과학기술원 Composition pharmaceutique comprenant un inhibiteur de hif-2α comme ingrédient actif pour la prévention ou le traitement de l'arthrite
WO2011145795A1 (fr) 2010-05-20 2011-11-24 광주과학기술원 Souris transgénique hif-2α spécifique du cartilage comme modèle animal pour l'arthrite, et son utilisation
EP2390352A1 (fr) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systemes et procedes pour accroitre la production de proteines et de lait chez des bovins laitiers
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
EP2442109A1 (fr) 2006-07-14 2012-04-18 The Regents of the University of California Biomarqueurs du cancer et procédé d'utilisation correspondant
EP2455489A2 (fr) 2006-05-25 2012-05-23 Monsanto Technology LLC Procédé pour identifier des locus de traits quantitatifs résistants aux maladies dans le soja et compositions correspondantes
WO2012090073A2 (fr) 2010-12-30 2012-07-05 The Netherlands Cancer Institute Procédés et compositions pour prédire la sensibilité à la chimiothérapie
US8222023B2 (en) 2006-03-15 2012-07-17 Micronics, Inc. Integrated nucleic acid assays
EP2476761A2 (fr) 2005-07-07 2012-07-18 Athlomics Pty Ltd Gènes de marqueur de polynucléotide et leur expression pour le diagnostic de l'endotoxine
WO2012109133A1 (fr) 2011-02-07 2012-08-16 Research Development Foundation Polypeptides fc modifiés d'immunoglobuline
WO2012120377A2 (fr) 2011-03-08 2012-09-13 King Abdullah University Of Science And Technology Biomarqueur moléculaire destiné à la détection précoce du cancer des ovaires
WO2012138783A2 (fr) 2011-04-04 2012-10-11 Netherlands Cancer Institute Procédés et compositions pour prédire une résistance à un traitement anti-cancéreux
WO2012138789A2 (fr) 2011-04-04 2012-10-11 Netherlands Cancer Institute Procédés et compositions pour prédire une résistance à un traitement anti-cancéreux
WO2012149038A1 (fr) 2011-04-25 2012-11-01 Advanced Bioscience Laboratories, Inc. Protéines tronquées d'enveloppe (env) du vih, procédés et compositions associés à celles-ci
WO2012158238A2 (fr) 2011-02-28 2012-11-22 University Of Iowa Research Foundation Changements d'hormone antimullérienne dans la grossesse et prédiction d'évolution indésirable de grossesse et du sexe
WO2013045505A1 (fr) 2011-09-28 2013-04-04 Novartis Ag Biomarqueurs pour traitement combiné par des agents sraa
WO2013071233A1 (fr) 2011-11-10 2013-05-16 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Procédés de détection d'agents infectieux et nouveau virus détecté par ces procédés
WO2013074676A2 (fr) 2011-11-14 2013-05-23 The General Hospital Corporation Dosages et procédés pour la sélection d'un schéma thérapeutique pour un sujet atteint d'une dépression
US8460867B2 (en) 2001-12-10 2013-06-11 Novartis Ag Methods of treating psychosis and schizophrenia based on polymorphisms in the CNTF gene
WO2013093629A2 (fr) 2011-12-20 2013-06-27 Netherlands Cancer Institute Vaccins modulaires, procédés et compositions qui y sont liés
WO2013096798A2 (fr) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Amplification d'une séquence provenant d'un acide ribonucléique
EP2623605A1 (fr) 2007-04-09 2013-08-07 University of Florida Research Foundation, Inc. Compositions de vecteur RAAV comprenant des protéines de capside à tyrosine modifié et procédés d'utilisation
WO2013116698A2 (fr) 2012-02-02 2013-08-08 Invenra, Inc. Crible haut débit pour des polypeptides biologiquement actifs
WO2013144249A1 (fr) 2012-03-29 2013-10-03 Novartis Ag Produit pharmaceutique à des fins diagnostiques
US8568730B2 (en) 2009-04-22 2013-10-29 Indiana University Research & Technology Corporation Compositions for use in the treatment of chronic obstructive pulmonary diseases and asthma
US8614062B1 (en) 2009-07-24 2013-12-24 University Of South Florida RNA-based system and method to differentiate seafood
US8722866B2 (en) 2001-08-01 2014-05-13 The United States Of America, As Represented By The Department Of Veterans Affairs Isoform-selective inhibitors and activators of PDE3 cyclic nucleotide phosphodiesterases
US8735066B2 (en) 1997-01-30 2014-05-27 Board Of Regents, The University Of Texas System Tumor suppressor designated TS10Q23.3
EP2740805A1 (fr) 2012-12-07 2014-06-11 SuppreMol GmbH Stratification et traitement de patients du purpura thrombocytopénique idiopathique
US8841432B2 (en) 2011-04-21 2014-09-23 Snu R&Db Foundation Shuttle vectors for mycobacteria-escherichia coli and uses thereof
WO2014148817A1 (fr) 2013-03-21 2014-09-25 주식회사 현일바이오 Procédé de détection sélective pour mycobacterium tuberculosis et des mycobactéries non tuberculeuses et kit utilisant celui-ci
US8859467B2 (en) 2009-07-17 2014-10-14 Bioatla, Llc Simultaneous, integrated selection and evolution of antibody/protein performance and expression in production hosts
WO2014171698A1 (fr) 2013-04-18 2014-10-23 사회복지법인 삼성생명공익재단 Méthode de diagnostic de la dystrophie myotonique de type 1
WO2014183023A1 (fr) 2013-05-09 2014-11-13 Trustees Of Boston University Utilisation de la plexine-a4 comme biomarqueur et cible thérapeutique pour la maladie d'alzheimer
EP2839837A1 (fr) 2006-09-15 2015-02-25 Ottawa Hospital Research Institute Rhabdovirus Oncolytique Farmington
WO2015105888A1 (fr) 2014-01-07 2015-07-16 Bioatla, Llc Orthologues ciblant les protéines
WO2015112767A2 (fr) 2014-01-22 2015-07-30 Life Technologies Corporation Nouvelles rétrotranscriptases pour une utilisation dans la synthèse d'acide nucléique à haute température
EP2913402A1 (fr) 2010-07-16 2015-09-02 Tocagen Inc. Détection de rétrovirus
EP2940027A1 (fr) 2004-07-08 2015-11-04 Corixa Corporation Composes d'iaminoalkylglucosaminide phosphate et leur utilisation
WO2015191916A1 (fr) 2014-06-11 2015-12-17 Micronics, Inc. Cartouches microfluidiques et appareil à témoins de dosage intégrés pour l'analyse d'acides nucléiques
WO2016086227A2 (fr) 2014-11-26 2016-06-02 The Regents Of The University Of California Compositions thérapeutiques comprenant des facteurs de transcription et leurs procédés de préparation et d'utilisation
US9409983B2 (en) 2009-07-23 2016-08-09 The Board Of Trustess Of The University Of Illinois Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases
EP3056566A1 (fr) 2005-01-28 2016-08-17 Life Technologies Corporation Inhibiteurs multi-composants de polymerases d'acides nucleiques et methodes associees
WO2016130516A1 (fr) 2015-02-09 2016-08-18 Research Development Foundation Polypeptides ayant un domaine fc d'immunoglobuline modifié manifestant une activation du complément améliorée
EP3093341A1 (fr) 2000-05-26 2016-11-16 Life Technologies Corporation Transcriptases inverses thermostables et utilisations
US9540670B2 (en) 2011-10-06 2017-01-10 bioMérieux Mutated T7 RNA Polymerases
WO2017015075A1 (fr) 2015-07-17 2017-01-26 President And Fellows Of Harvard College Procédés d'amplification de séquences d'acides nucléiques
WO2017040380A2 (fr) 2015-08-28 2017-03-09 Research Development Foundation Variants de fc d'anticorps modifiés
EP3249054A1 (fr) 2012-12-20 2017-11-29 Biomatrica, INC. Formulations et procédés pour stabiliser des réactifs pcr
WO2017214068A1 (fr) 2016-06-05 2017-12-14 Berg Llc Systèmes et procédés de stratification de patient et identification de biomarqueurs potentiels
US9895692B2 (en) 2010-01-29 2018-02-20 Micronics, Inc. Sample-to-answer microfluidic cartridge
US9896664B2 (en) 2009-12-10 2018-02-20 Turnstone Limited Partnership Oncolytic rhabdovirus
US9919047B2 (en) 2011-01-04 2018-03-20 Sillajen, Inc. Generation of antibodies to tumor antigens and generation of tumor specific complement dependent cytotoxicity by administration of oncolytic vaccinia virus
WO2018128516A1 (fr) 2017-01-09 2018-07-12 연세대학교 산학협력단 Composition pharmaceutique pour un traitement de précancer buccal et procédé de prédiction ou de diagnostic d'un précancer buccal ou d'un cancer buccal
WO2018140606A1 (fr) 2017-01-26 2018-08-02 Oklahoma Medical Research Foundation Biomarqueurs de l'activité, de l'intensité et de l'éruption de la maladie du lupus érythémateux disséminé
US10040853B2 (en) 2011-09-09 2018-08-07 Fred Hutchinson Cancer Research Center Methods and compositions involving NKG2D inhibitors and cancer
US10065186B2 (en) 2012-12-21 2018-09-04 Micronics, Inc. Fluidic circuits and related manufacturing methods
US10087440B2 (en) 2013-05-07 2018-10-02 Micronics, Inc. Device for preparation and analysis of nucleic acids
US10125373B2 (en) 2013-01-22 2018-11-13 Arizona Board Of Regents On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
WO2018219997A1 (fr) 2017-05-30 2018-12-06 Roche Diagnostics Gmbh Catalyseurs pour inverser des produits d'addition de formaldéhyde et des réticulations
US10172879B2 (en) 2015-01-21 2019-01-08 D.R.Nano Co., Ltd Nanocomplexes for co-delivering a drug and siRNA and uses thereof
WO2019017680A2 (fr) 2017-07-19 2019-01-24 국민대학교 산학협력단 Micro-arn utilisé en tant que biomarqueur de la maladie de parkinson et kit diagnostique le mettant en oeuvre
US10190153B2 (en) 2013-05-07 2019-01-29 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
US10363293B2 (en) 2013-02-21 2019-07-30 Turnstone Limited Partnership Vaccine composition
US10386377B2 (en) 2013-05-07 2019-08-20 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
US10393739B2 (en) 2013-10-03 2019-08-27 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
US10436713B2 (en) 2012-12-21 2019-10-08 Micronics, Inc. Portable fluorescence detection system and microassay cartridge
US10518262B2 (en) 2012-12-21 2019-12-31 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
WO2020089841A1 (fr) 2018-10-31 2020-05-07 Garcia Joe G N Biomarqueurs et méthodes d'utilisation de ces biomarqueurs pour détecter une lésion pulmonaire radio-induite
US10772951B2 (en) 2011-06-08 2020-09-15 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
WO2020223576A1 (fr) 2019-04-30 2020-11-05 Chondrial Therapeutics, Inc. Marqueurs sensibles à la frataxine pour déterminer l'efficacité d'une thérapie de remplacement de frataxine
WO2021246820A1 (fr) 2020-06-05 2021-12-09 주식회사 씨젠 Kit de transport d'échantillon pour la détection d'agents pathogènes respiratoires et procédés de détection d'agents pathogènes respiratoires à l'aide de celui-ci
WO2022047359A1 (fr) 2020-08-31 2022-03-03 Berg Llc Biomarqueurs protéiques du cancer du pancréas
US11339421B2 (en) 2016-02-23 2022-05-24 Industry Foundation Of Chonnam National University Leukemia diagnostic kit targeting prohibitin gene and diagnostic method using same
US11447772B2 (en) 2017-07-19 2022-09-20 Kookmin University Industry Academy Cooperation Foundation miRNA as biomarker for Parkinson's disease and diagnostic kit using same
WO2022216841A1 (fr) 2021-04-06 2022-10-13 Berg Llc Marqueurs protéiques pour un cancer du sein positif au récepteur des œstrogènes (er) de type luminal a (la) et de type luminal b1 (lb1)
WO2022216846A1 (fr) 2021-04-06 2022-10-13 Berg Llc Marqueurs protéiques pour le cancer du sein du type positif aux récepteurs des oestrogènes (er) et du type négatif aux récepteurs des oestrogènes (er)
WO2022265965A1 (fr) 2021-06-14 2022-12-22 10X Genomics, Inc. Variants de la transcriptase inverse pour une performance améliorée
US11543411B2 (en) 2014-12-05 2023-01-03 Prelude Corporation DCIS recurrence and invasive breast cancer
EP4242329A2 (fr) 2014-12-08 2023-09-13 Berg LLC Utilisation de marqueurs comprenant de la filamine a dans le diagnostic et le traitement du cancer de la prostate
WO2023196937A1 (fr) 2022-04-06 2023-10-12 Larimar Therapeutics, Inc. Marqueurs sensibles à la frataxine pour surveiller une thérapie de remplacement de frataxine
EP4275684A1 (fr) 2018-05-25 2023-11-15 Arca Biopharma, Inc. Procédés et compositions impliquant du bucindolol pour le traitement de la fibrillation auriculaire
US11821900B2 (en) 2018-09-14 2023-11-21 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
WO2023230531A1 (fr) 2022-05-24 2023-11-30 Lunglife Ai, Inc. Procédés de détection de cellules génétiquement anormales circulantes
WO2023239940A1 (fr) 2022-06-10 2023-12-14 Research Development Foundation Variants de fc igg1 sélectifs de fcriib modifiés et leurs utilisations

Families Citing this family (534)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340807C (fr) * 1988-02-24 1999-11-02 Lawrence T. Malek Procede d'amplification d'une sequence d'acide nucleique
JP2650159B2 (ja) * 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
AU633036B2 (en) * 1988-12-09 1993-01-21 Chemicon International, Inc. Amplified dna assay
IE66597B1 (en) * 1989-05-10 1996-01-24 Akzo Nv Method for the synthesis of ribonucleic acid (RNA)
CA2020958C (fr) * 1989-07-11 2005-01-11 Daniel L. Kacian Methodes d'amplification de sequences d'acide nucleique
WO1991004340A1 (fr) * 1989-09-20 1991-04-04 Cambridge Biotech Corporation Amplification isothermique in vitro de l'acide nucleique
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
IE65771B1 (en) * 1990-01-25 1995-11-15 Zeneca Ltd Amplification of nucleotide sequences using vectorette units
WO1991013994A1 (fr) * 1990-03-13 1991-09-19 Commonwealth Scientific And Industrial Research Organisation Expression de genes
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
ATE176002T1 (de) * 1990-07-24 1999-02-15 Hoffmann La Roche Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
NZ240079A (en) * 1990-10-09 1993-07-27 Boehringer Mannheim Gmbh Method for the detection of a nucleic acid or part thereof
RU2017821C1 (ru) * 1990-10-10 1994-08-15 Анатолий Михайлович Онищенко Способ амплификации днк и устройство для его осуществления
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
WO1992018521A1 (fr) * 1991-04-10 1992-10-29 Life Technologies, Inc. Procede d'amplification et de modification d'une sequence d'arn
WO1992020820A1 (fr) * 1991-05-15 1992-11-26 Vanderbilt University Methode de determination du potentiel metastatique des cellules tumorales
EP0517361A1 (fr) * 1991-05-30 1992-12-09 Amoco Corporation Méthode de détection et identification d'organismes pathogènes en utilisant des séquences cibles comme détecteurs
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5137814A (en) * 1991-06-14 1992-08-11 Life Technologies, Inc. Use of exo-sample nucleotides in gene cloning
US5229283A (en) * 1991-06-14 1993-07-20 Life Technologies, Inc. Use of exo-sample nucleotides in gene cloning
DE4129653A1 (de) * 1991-09-06 1993-03-11 Boehringer Mannheim Gmbh Verfahren zum nachweis aehnlicher nukleinsaeuren
DE4132133A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen herstellung von ribonukleinsaeuren
DE4213029A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen
US5981179A (en) * 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
US6100099A (en) 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
WO1993023560A1 (fr) * 1992-05-11 1993-11-25 Schuepbach Joerg Procede de mise en evidence de la transcriptase reverse
US5843640A (en) * 1992-06-19 1998-12-01 Northwestern University Method of simultaneously detecting amplified nucleic acid sequences and cellular antigens in cells
US5580971A (en) * 1992-07-28 1996-12-03 Hitachi Chemical Company, Ltd. Fungal detection system based on rRNA probes
JPH07509361A (ja) * 1992-07-28 1995-10-19 日立化成工業株式会社 遺伝子検出システム
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5614389A (en) * 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
WO1994003624A1 (fr) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Procedes d'amplification isothermique de molecules d'acide nucleique
US5733733A (en) * 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en) * 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
ZA936015B (en) * 1992-08-24 1994-03-10 Akzo Nv Elimination of false negatives in nuleic acid detection.
ZA936016B (en) * 1992-08-24 1994-03-10 Akzo Nv Method for nucleic acid amplification
DE4236708A1 (de) * 1992-10-30 1994-05-05 Bayer Ag Spezifische Gensonden und Verfahren zur Diagnostik von Candida albicans
DE4302459A1 (de) * 1993-01-29 1994-08-04 Bayer Ag Sulfocumarinhaltige Nukleotide und deren Verwendung in Nachweisverfahren für Nukleinsäuren
DE4344742A1 (de) * 1993-06-09 1994-12-15 Boehringer Mannheim Gmbh Verfahren zur Immobilisierung von Nukleinsäuren
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
GB2284209A (en) * 1993-11-25 1995-05-31 Ole Buchardt Nucleic acid analogue-induced transcription of RNA from a double-stranded DNA template
WO1995015399A1 (fr) 1993-12-01 1995-06-08 Toyo Boseki Kabushiki Kaisha Procede d'amplification et de detection d'une sequence nucleotidique au moyen d'enzymes thermostables
WO2000033244A2 (fr) * 1998-11-27 2000-06-08 Synaptics (Uk) Limited Capteur de position
US6010847A (en) 1994-08-18 2000-01-04 Akzo Nobel N.V. Oligonucleotides that can be used in the amplification and detection of CMV nucleic acid
US5656427A (en) * 1994-08-29 1997-08-12 Gen-Probe Incorporated Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5849879A (en) * 1994-11-03 1998-12-15 The Regents Of The University Of California Methods for the diagnosis of glaucoma
US5654141A (en) * 1994-11-18 1997-08-05 Thomas Jefferson University Amplification based detection of bacterial infection
US6001610A (en) * 1994-11-23 1999-12-14 Roche Diagnostics, Gmbh Method for the particularly sensitive detection of nucleic acids
DE4441626A1 (de) * 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
EP0799888B1 (fr) * 1994-12-09 2005-05-18 Wakunaga Seiyaku Kabushiki Kaisha Procede d'inhibition de l'hybridation non specifique dans une extension d'amorce
CA2139070C (fr) * 1994-12-23 2010-03-30 Burton W. Blais Methode pour ameliorer l'aptitude a la detection d'essais d'acide nucleique utilisant une reaction en chaine de polymerase
US5925518A (en) * 1995-05-19 1999-07-20 Akzo Nobel N.V. Nucleic acid primers for amplification of a mycobacteria RNA template
US5856096A (en) * 1995-09-20 1999-01-05 Ctrc Research Foundation Rapid and sensitive assays for detecting and distinguishing between processive and non-processive telomerase activities
US5747255A (en) * 1995-09-29 1998-05-05 Lynx Therapeutics, Inc. Polynucleotide detection by isothermal amplification using cleavable oligonucleotides
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
JP3974941B2 (ja) 1995-11-21 2007-09-12 イェール ユニバーシティ 単分子セグメントの増幅および検出
US6048688A (en) * 1996-11-12 2000-04-11 Kimberly-Clark Corporation Method for detection of Pseudomonas aeruginosa using polymerase chain reaction
US7195871B2 (en) * 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US20080160524A1 (en) * 1996-01-24 2008-07-03 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
EP0914462A4 (fr) * 1996-03-18 2002-05-22 Molecular Biology Resources Amplification de sequences d'acides nucleiques cibles
US6759217B2 (en) 1996-03-26 2004-07-06 Oncomedx, Inc. Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer
CA2250118C (fr) 1996-03-26 2009-09-29 Michael S. Kopreski Procede permettant d'employer de l'arn extracellulaire extrait de plasma ou de serum a la detection, a la surveillance ou a l'evaluation d'un cancer
US7785842B2 (en) * 1996-03-26 2010-08-31 Oncomedx, Inc. Comparative analysis of extracellular RNA species
US8043835B1 (en) 1996-03-26 2011-10-25 Oncomedx, Inc. Methods for detecting and monitoring cancer using extracellular RNA
US5712127A (en) * 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
US6436638B1 (en) 1996-05-09 2002-08-20 Metropolitan Water District Of Southern California Cryptosporidium detection method
US5770368A (en) * 1996-05-09 1998-06-23 Metropolitan Water District Of Southern California Cryptosporidium detection method
US5876992A (en) 1996-07-03 1999-03-02 Molecular Biology Resources, Inc. Method and formulation for stabilization of enzymes
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
JPH1028585A (ja) * 1996-07-16 1998-02-03 Toyobo Co Ltd 耐熱性リボヌクレアーゼｈを用いる核酸増幅法
US6537783B1 (en) 1996-08-02 2003-03-25 Bio Merieux Prefunctionalized nucleotide and process for amplifying a sequence using a prefunctionalized nucleotide
WO1998008981A1 (fr) * 1996-08-30 1998-03-05 Life Technologies, Inc. PROCEDES D'IDENTIFICATION ET D'ISOLEMENT DE SEQUENCES DE NUCLEOTIDES SPECIFIQUES DANS L'ADNc ET L'ADN GENOMIQUE
US6043283A (en) * 1996-09-20 2000-03-28 Baylor College Of Medicine Tyramine compounds and their neuronal effects
US6071493A (en) 1996-09-20 2000-06-06 Baylor College Of Medicine Method of screening for an agent that inhibits mononuclear phagocyte-plaque component complex formation
US6096273A (en) * 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US7381525B1 (en) * 1997-03-07 2008-06-03 Clinical Micro Sensors, Inc. AC/DC voltage apparatus for detection of nucleic acids
AU746549B2 (en) 1996-11-20 2002-05-02 Becton Dickinson & Company Microfabricated isothermal nucleic acid amplification devices and methods
US20040142327A1 (en) * 1996-11-22 2004-07-22 Jhy-Jhu Lin Methods for identifying and isolating DNA polymorphisms or genetic markers
JP2001524808A (ja) 1996-12-10 2001-12-04 ジーントレイス・システムズ・インコーポレイテッド 放出可能な不揮発性の質量標識分子
US8440396B2 (en) * 1997-03-14 2013-05-14 Oncomedx, Inc. Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer
US20070009934A1 (en) * 1997-03-14 2007-01-11 Kopreski Michael S Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer
US6204024B1 (en) 1997-09-12 2001-03-20 Akzo Nobel N.V. CCR5 RNA transcription based amplification assay
US6461817B1 (en) 1997-09-12 2002-10-08 The Public Health Research Institute Of The City Of New York Non-competitive co-amplification methods
ZA989950B (en) * 1997-11-17 1999-05-04 Akzo Nobel Nv Transcription based amplification of double stranded DNA targets
US5968732A (en) * 1997-12-31 1999-10-19 Akzo Nobel, N.V. Isothermal transcription based assay for the detection and genotyping of dengue virus
US6093542A (en) * 1998-01-09 2000-07-25 Akzo Nobel N.V. Isothermal transcription based amplification assay for the detection and quantitation of macrophage derived chemokine RNA
US6121023A (en) * 1998-01-22 2000-09-19 Akzo Nobel N.V. Isothermal transcription based assay for the detection and quantification of the chemokine rantes
US6673914B1 (en) 1998-01-22 2004-01-06 John Wayne Cancer Institute Human tumor-associated gene
US6686150B1 (en) 1998-01-27 2004-02-03 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
JP2002500897A (ja) 1998-01-27 2002-01-15 クリニカル・マイクロ・センサーズ・インコーポレイテッド 電子的核酸検出の増幅
USH1825H (en) * 1998-01-30 1999-12-07 Akzo Nobel N.V. Isothermal transcription based assay for the detection of HTLV I and HTLV II RNA
US6365346B1 (en) * 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
ATE429517T1 (de) * 1998-03-04 2009-05-15 Biomerieux Bv Vervielfältigung und nachweis von ebv barf1 zur diagnose von nasopharyngealen karzinom
US20050244954A1 (en) * 1998-06-23 2005-11-03 Blackburn Gary F Binding acceleration techniques for the detection of analytes
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法
US6316229B1 (en) 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
CA2342838A1 (fr) 1998-09-15 2000-03-23 Yale University Clonage moleculaire a amplification selon le modele du cercle roulant
CA2342837A1 (fr) * 1998-09-15 2000-03-23 Yale University Long segment terminal artificiel a repetitions
US8163524B2 (en) * 1998-09-22 2012-04-24 Oncomedx, Inc. Comparative analysis of extracellular RNA species
US20060204989A1 (en) * 1998-09-22 2006-09-14 Kopreski Michael S Comparative analysis of extracellular RNA species
US20090233276A1 (en) * 1998-09-22 2009-09-17 Oncomedx, Inc. Method Enabling the Use of Extracellular Ribonucleic Acid (RNA) Extracted from Plasma or Serum to Detect, Monitor or Evaluate Cancer or Premalignant Conditions
US6743906B1 (en) 1998-10-02 2004-06-01 Board Of Regents, The University Of Texas PPP2R1B is a tumor suppressor
US6153411A (en) 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification
EP1829856A3 (fr) * 1998-11-12 2009-02-25 Invitrogen Corporation Réactifs de transfection
US6187566B1 (en) 1999-03-09 2001-02-13 Applied Gene Technologies, Inc. Method of labeling a nucleic acid amplicon with simultaneous contamination prevention
WO2000056916A2 (fr) * 1999-03-18 2000-09-28 Exiqon A/S Detection de mutations dans des genes par des amorces de lna specifiques
US6630333B1 (en) * 1999-03-23 2003-10-07 Invitrogen Corporation Substantially pure reverse transriptases and methods of production thereof
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
US6582906B1 (en) 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids
US6235479B1 (en) 1999-04-13 2001-05-22 Bio Merieux, Inc. Methods and devices for performing analysis of a nucleic acid sample
EP1923471B1 (fr) 1999-04-20 2012-12-19 Illumina, Inc. Détection de réactions d'acide nucléique sur les réseaux à perle
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
WO2000068411A1 (fr) * 1999-05-12 2000-11-16 Invitrogen Corporation Compositions et methodes d'amelioration de la sensibilite et de la specificite d'une synthese d'acide nucleique
US8080380B2 (en) * 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
NZ515673A (en) * 1999-05-21 2004-03-26 Invitrogen Corp Compositions and methods for labeling of nucleic acid molecules
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6495320B1 (en) 1999-07-21 2002-12-17 Affymetrix, Inc. Even length proportional amplification of nucleic acids
CA2380258C (fr) 1999-07-26 2008-07-15 Clinical Micro Sensors, Inc. Determination de sequences d'acides nucleiques par detection electronique
US6864050B2 (en) 1999-07-30 2005-03-08 Affymetrix, Inc. Single-phase amplification of nucleic acids
US6242188B1 (en) 1999-07-30 2001-06-05 Applied Gene Technologies, Inc. Sample processing to release nucleic acids for direct detection
US6379930B1 (en) 1999-07-30 2002-04-30 Applied Gene Technologies, Inc. Stabilization of nucleic acid amplification cocktails
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6794138B1 (en) 1999-12-16 2004-09-21 Affymetrix, Inc. Methods of small sample amplification
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US20050214825A1 (en) * 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) * 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US6291187B1 (en) 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
EP1158055A1 (fr) 2000-05-26 2001-11-28 Xu Qi University of Teaxs Laboratoire de Leucémie Chen Méthode pour le diagnostic de cancers
US20060166227A1 (en) * 2000-06-20 2006-07-27 Stephen Kingsmore Protein expression profiling
AU2001271595A1 (en) * 2000-06-26 2002-01-08 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
AU7172201A (en) 2000-06-30 2002-01-14 Molecular Staging Inc Signal amplification with lollipop probes
JP2002142765A (ja) * 2000-07-14 2002-05-21 Tosoh Corp 新規ゲノム解析法
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002020852A1 (fr) 2000-09-01 2002-03-14 Gen-Probe Incorporated Amplification de sequences vih-1 pour la detection de sequences associees aux mutations de la resistance aux medicaments
EP1366192B8 (fr) * 2000-10-24 2008-10-29 The Board of Trustees of the Leland Stanford Junior University Caracterisation multiplex directe d'adn genomique
JP2005503755A (ja) * 2000-12-13 2005-02-10 ニューゲン テクノロジーズ， インコーポレイテッド 核酸配列の複数コピーを作製するための方法および組成物、ならびにそれらを検出する方法
US6794141B2 (en) * 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
US20040185455A1 (en) * 2000-12-26 2004-09-23 Masamitsu Shimada Method of detecting pathogenic microorganism
US6893847B2 (en) * 2001-01-17 2005-05-17 Tosoh Corporation Oligonucleotide for detecting Salmonella and method of detecting Salmonella
JPWO2002062993A1 (ja) * 2001-02-06 2004-06-10 タカラバイオ株式会社 増幅核酸及びその固定化物
KR100809949B1 (ko) * 2001-02-15 2008-03-06 다카라 바이오 가부시키가이샤 염기 치환의 검출 방법
US6620586B2 (en) * 2001-02-20 2003-09-16 Applied Gene Technologies, Inc. Methods and compositions for analyzing nucleic acids
EP1381697B1 (fr) * 2001-02-27 2009-03-18 Virco Bvba Amplification de sonde circulaire (asc) de molecules d'acide nucleique circularisees
CA2416963C (fr) * 2001-03-09 2012-01-10 Nugen Technologies, Inc. Procedes et compositions permettant l'amplification de sequences d'arn
US6573051B2 (en) * 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
WO2002072773A2 (fr) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methodes et compositions pour amplification de sequences d'arn
US20030092157A1 (en) * 2001-03-16 2003-05-15 Hayden Michael R. Compositions, screening systems and methods for modulating HDL cholesterol and triglyceride levels
US6596489B2 (en) 2001-03-30 2003-07-22 Applied Gene Technologies Methods and compositions for analyzing nucleotide sequence mismatches using RNase H
AU2003303395A1 (en) * 2001-05-22 2004-07-22 Dahl, Gary, A. Target-dependent transcription using deletion mutants of n4 rna polymerase
CA2448484A1 (fr) * 2001-05-25 2002-12-05 Xenon Genetics, Inc. Procedes de diagnostic de maladies cardio-vasculaires, faibles niveaux de cholesterol ldl, et niveaux eleves de triglyceride
CN100379861C (zh) * 2001-06-12 2008-04-09 宝生物工程株式会社 核酸扩增或检测用试剂的稳定化方法和保存方法
BR0210610A (pt) * 2001-06-22 2005-04-19 Marshfield Clinic Métodos e oligonucleotìdeos para detecção de salmonella sp., e.coli 0157:h7 e listeria monocitogenes
DE60229958D1 (de) * 2001-06-28 2009-01-02 Mountain View Pharmaceuticals Polymerstabilisierte proteinasen
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US9777312B2 (en) * 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
WO2003006677A2 (fr) 2001-07-12 2003-01-23 Illumina, Inc. Reactions multiplex d'acides nucleiques
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
DK1421215T3 (da) * 2001-07-25 2011-06-27 Oncomedx Inc Fremgangsmåder til evaluering af patologiske tilstande under anvendelse af ekstracellulært RNA
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
TWI335938B (en) * 2001-08-15 2011-01-11 Rna replication and amplification
KR20040028991A (ko) * 2001-08-20 2004-04-03 다카라 바이오 가부시키가이샤 핵산 증폭 방법
WO2003020979A1 (fr) * 2001-08-31 2003-03-13 Rosetta Inpharmactis Llc. Procedes de preparation d'echantillons d'acide nucleique utiles pour cribler des reseaux d'adn
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US20030104454A1 (en) * 2001-11-05 2003-06-05 Kopreski Michael S. Method for detection of DNA methyltransferase RNA in plasma and serum
US20100159464A1 (en) * 2001-11-05 2010-06-24 Oncomedx, Inc. Method for Detection of DNA Methyltransferase RNA in Plasma and Serum
DE60228995D1 (de) * 2001-12-19 2008-10-30 Affymetrix Inc Arrayplatten und verfahren zur herstellung von arrayplatten
WO2003064679A2 (fr) * 2002-01-30 2003-08-07 Id Biomedical Corporation Procedes pour detecter des microorganismes resistant a la vancomycine et composition pour la mise en oeuvre dudit procede
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US7354742B2 (en) 2002-02-22 2008-04-08 Ortho-Mcneil Pharmaceutical, Inc. Method for generating amplified RNA
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US20060246434A1 (en) * 2002-03-15 2006-11-02 Erlander Mark G Nucleic acid amplification
WO2004011665A2 (fr) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Procedes de fragmentation, d'etiquetage et d'immobilisation d'acides nucleiques
US20030219754A1 (en) * 2002-05-23 2003-11-27 Oleksy Jerome E. Fluorescence polarization detection of nucleic acids
US20030219755A1 (en) * 2002-05-24 2003-11-27 Nanibhushan Dattagupta Compositions and methods for performing hybridization assays using target enhanced signal amplification (TESA)
US20040219516A1 (en) * 2002-07-18 2004-11-04 Invitrogen Corporation Viral vectors containing recombination sites
US20040011650A1 (en) * 2002-07-22 2004-01-22 Frederic Zenhausern Method and apparatus for manipulating polarizable analytes via dielectrophoresis
EP1546392A4 (fr) * 2002-08-02 2006-05-24 Stratatech Corp Detection d'adn specifique d'espece
WO2004013290A2 (fr) * 2002-08-05 2004-02-12 Invitrogen Corporation Compositions et procedes applicables a la biologie moleculaire
EP1539209A2 (fr) * 2002-09-05 2005-06-15 Invitrogen Corporation Compositions et procedes de synthese d'acides nucleiques
AU2002951411A0 (en) 2002-09-16 2002-09-26 The University Of Sydney Genotype screen
JP2006500030A (ja) 2002-09-20 2006-01-05 イェール ユニバーシティ リボスイッチ、その使用方法、ならびにリボスイッチとともに用いるための組成物
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
US7074561B2 (en) * 2002-10-22 2006-07-11 Biomerieux, Inc. Isothermal amplification based assay for the detection and quantitation of alpha-fetoprotein mRNA
US20040096829A1 (en) * 2002-11-14 2004-05-20 Allaire Normand E. Absolute quantitation of nucleic acids by RT-PCR
AU2003297557B2 (en) * 2002-11-21 2009-02-26 Cellscript, Inc. Methods for using primers that encode one strand of a double-stranded promoter
CN1230531C (zh) * 2002-12-09 2005-12-07 清华大学 从样品中分离细胞粒子的方法
CN1223680C (zh) 2002-12-10 2005-10-19 清华大学 扩增靶细胞或病毒的核酸的方法
EP1573009B1 (fr) 2002-12-18 2011-09-21 Third Wave Technologies, Inc. Detection de petits acides nucleiques
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US7297780B2 (en) 2003-01-06 2007-11-20 Third Wave Technologies, Inc. Reactive functional groups for preparation of modified nucleic acid
US6852494B2 (en) * 2003-01-10 2005-02-08 Linden Technologies, Inc. Nucleic acid amplification
WO2004083806A2 (fr) 2003-01-22 2004-09-30 University Of South Florida Appareil a genocapteur autonome et procedes d'utilisation de celui-ci
US6943768B2 (en) 2003-02-21 2005-09-13 Xtellus Inc. Thermal control system for liquid crystal cell
US20060192960A1 (en) * 2003-03-24 2006-08-31 Rencs Erik V Polarization detection
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
JP4782668B2 (ja) 2003-03-31 2011-09-28 エフ．ホフマン−ラ ロシュ アーゲー 日本脳炎ウィルス血清グループのメンバーを包含する一定のフラビウィルスを検出するための組成物及び方法
US20040265870A1 (en) * 2003-04-09 2004-12-30 Invitrogen Corporation Methods of synthesizing and labeling nucleic acid molecules
WO2004092418A2 (fr) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Amplification globale effectuee avec une amorce composite amorcee de maniere aleatoire
CA2528572C (fr) 2003-06-10 2020-08-25 The Trustees Of Boston University Analyse de l'expression genetique des cellules epitheliales de voies aeriennes pour diagnostiquer un cancer du poumon
EP2216415B2 (fr) * 2003-08-01 2017-01-04 Life Technologies Corporation Compositions et procédés de préparation de courtes molécules d'ARN et d'autres acides nucléiques
JP4790621B2 (ja) 2003-11-26 2011-10-12 アドバンディーエックス， インコーポレイテッド 特定のＳｔａｐｈｙｌｏｃｏｃｃｕｓ種の分析のためのペプチド核酸プローブ
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US20050208538A1 (en) 2003-12-29 2005-09-22 Nurith Kurn Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
WO2005076908A2 (fr) * 2004-02-04 2005-08-25 Eppendorf Ag Amplification d'acide nucleique ameliore de proteine antigel
EP1564306B1 (fr) 2004-02-17 2013-08-07 Affymetrix, Inc. Procédé de fragmentation et de marquage d'ADN
EP1574583A1 (fr) * 2004-03-10 2005-09-14 Roche Diagnostics GmbH Procédé pour isoler des bactéries des échantillons biologiques
US7462451B2 (en) * 2004-04-26 2008-12-09 Third Wave Technologies, Inc. Compositions for modifying nucleic acids
ES2560433T3 (es) 2004-04-26 2016-02-19 Wako Pure Chemical Industries, Ltd. Cebadores para la detección del bacilo de la tuberculosis y procedimiento de detección del bacilo de la tuberculosis humana con los mismos
CN103884698B (zh) 2004-06-07 2017-04-12 先锋生物科技股份有限公司 用于微流体器件的光学透镜系统和方法
US7776530B2 (en) * 2004-06-29 2010-08-17 Wallac Oy Integrated nucleic acid analysis
US20090269737A1 (en) * 2004-06-29 2009-10-29 Wallac Oy Integrated non-homogeneous nucleic acid amplification and detection
EP1767655A4 (fr) 2004-07-13 2007-08-01 Takeda Pharmaceutical Méthode de contrôle des fonctions d'une cellule
WO2006026388A2 (fr) 2004-08-27 2006-03-09 Gen-Probe Incorporated Techniques d'amplification d'acide nucleique avec une seule amorce
US7713697B2 (en) * 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
US20060073506A1 (en) 2004-09-17 2006-04-06 Affymetrix, Inc. Methods for identifying biological samples
US20060068430A1 (en) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Purification of biomolecules from contaminating intact nucleic acids
US20060073511A1 (en) 2004-10-05 2006-04-06 Affymetrix, Inc. Methods for amplifying and analyzing nucleic acids
US7682782B2 (en) 2004-10-29 2010-03-23 Affymetrix, Inc. System, method, and product for multiple wavelength detection using single source excitation
EP1652580A1 (fr) 2004-10-29 2006-05-03 Affymetrix, Inc. Microréseaux à haut débit, ensembles réseaux et procédé de leurs fabrications
CA2587670A1 (fr) 2004-11-01 2006-05-11 George Mason University Compositions et methodes pour le diagnostic de troubles du colon
JP2008522588A (ja) * 2004-12-06 2008-07-03 バイオヴェリス コーポレイション 炭疽菌（Ｂａｃｉｌｌｕｓａｎｔｈｒａｃｉｓ）を検出するための方法及び組成物
EP1819828A2 (fr) * 2004-12-11 2007-08-22 CytoGenix, Inc. Biosynthese acellulaire d'acide nucleique de haute qualite et utilisations correspondantes
EP1841879A4 (fr) 2005-01-25 2009-05-27 Population Genetics Technologi Amplification isotherme d'adn
DK2529619T3 (en) 2005-02-17 2016-01-11 Biogen Ma Inc Treatment of neurological disorders
CN101128604A (zh) 2005-02-28 2008-02-20 生物探索公司 用于进行涉及核酸分子的直接酶反应的方法
PL1863519T3 (pl) 2005-03-31 2014-03-31 Massachusetts Gen Hospital Modulowanie aktywności HGF/HGFR do leczenia obrzęku wskutek niedrożności naczyń chłonnych
US20060223071A1 (en) * 2005-04-01 2006-10-05 Wisniewski Michele E Methods, compositions, and kits for detecting nucleic acids in a single vessel
EP1863908B1 (fr) 2005-04-01 2010-11-17 Qiagen GmbH Transcription inverse et amplification d'arn avec degradation simultanee d'adn
CA2605158A1 (fr) 2005-04-14 2006-10-26 The Trustees Of Boston University Diagnostic des troubles pulmonaires mettant en oeuvre la prediction de categories
US20060240442A1 (en) * 2005-04-20 2006-10-26 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
US7550264B2 (en) 2005-06-10 2009-06-23 Datascope Investment Corporation Methods and kits for sense RNA synthesis
US7838646B2 (en) 2005-08-18 2010-11-23 Quest Diagnostics Investments Incorporated Cystic fibrosis transmembrane conductance regulator gene mutations
EP1929046B1 (fr) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Procedure d'amplication d'acide nucleique amelioree
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
EP1762627A1 (fr) 2005-09-09 2007-03-14 Qiagen GmbH Procédé pour l'activation d'acides nucléiques pour effectuer une réaction d'une polymérase
WO2007044671A2 (fr) * 2005-10-06 2007-04-19 Lucigen Corporation Polymerases virales thermostables, et leurs methodes d'utilisation
US8609829B2 (en) * 2005-10-17 2013-12-17 Gen-Probe Incorporated Compositions and methods to detect Legionella pneumophila nucleic acid
US7422857B2 (en) 2005-10-28 2008-09-09 University Of South Florida Detection of polyketide synthetase gene expression in Karenia brevis
US20070207455A1 (en) * 2005-11-17 2007-09-06 Third Wave Technologies, Inc. Compositions and methods for detecting an HCV-1 subtype
US7981606B2 (en) * 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
JP2009524411A (ja) 2005-12-21 2009-07-02 イェール ユニバーシティー リボスイッチの調節に関連した方法および組成物
WO2008018904A2 (fr) 2006-01-18 2008-02-14 Argos Therapeutics, Inc. Systèmes et procédés destinés au traitement d'échantillons dans un réceptacle fermé, et dispositifs associés
WO2007087262A2 (fr) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Amplification sélective du génome
US20090176878A1 (en) * 2007-10-05 2009-07-09 Washington University In St. Louis Genetic polymorphisms and substance dependence
US20110143344A1 (en) * 2006-03-01 2011-06-16 The Washington University Genetic polymorphisms and substance dependence
AU2007223788B2 (en) 2006-03-09 2012-11-29 The Trustees Of Boston University Diagnostic and prognostic methods for lung disorders using gene expression profiles from nose epithelial cells
US7947441B2 (en) * 2006-04-14 2011-05-24 University Of South Florida Molecular detection and quantification of Enterococci
EP2522751B1 (fr) 2006-06-01 2018-11-21 Third Wave Technologies, Inc. Détection d'acides nucléiques à l'aide d'un essai INVADER amélioré
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
EP2050828A3 (fr) 2006-06-12 2009-08-26 Oncomethylome Sciences S.A. Marqueurs de méthylation destinés à une détection et un pronostic précoces de cancers du colon
EP2038429B1 (fr) * 2006-06-30 2013-08-21 Nugen Technologies, Inc. Procédés de fragmentation et de marquage d'acides nucléiques
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
CA2660286A1 (fr) 2006-08-09 2008-02-21 Homestead Clinical Corporation Proteines specifiques d'organes et procedes d'utilisation
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
EP1914303A1 (fr) * 2006-10-09 2008-04-23 Qiagen GmbH ADN polymerases de Thermus eggertssonii
US9845494B2 (en) 2006-10-18 2017-12-19 Affymetrix, Inc. Enzymatic methods for genotyping on arrays
US8026065B2 (en) 2006-11-02 2011-09-27 Yale University Assessment of oocyte competence
WO2008079972A2 (fr) 2006-12-20 2008-07-03 Bayer Healthcare Llc Composé d'hydroxy méthyle phényle pyrazolyle urée utilisé pour traiter un cancer
US7794937B2 (en) 2006-12-22 2010-09-14 Quest Diagnostics Investments Incorporated Cystic fibrosis transmembrane conductance regulator gene mutations
CA2673772A1 (fr) * 2007-01-17 2008-07-24 Meridian Bioscience, Inc. Reactifs stables et necessaires intervenant dans l'amplification isotherme induite par la boucle (lamp)
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
EP1956097A1 (fr) 2007-02-06 2008-08-13 bioMerieux B.V. Procédé de discrimination des polymorphismes mononucléotidiques (SNPs)
US8183359B2 (en) * 2007-03-01 2012-05-22 Gen-Probe Incorporated Kits for amplifying DNA
JP2010520750A (ja) * 2007-03-01 2010-06-17 ジェン−プロウブ インコーポレイテッド Ｄｎａを増幅するための方法およびキット
EP1970440A1 (fr) * 2007-03-06 2008-09-17 Qiagen GmbH Stabilisation de polymérase par des détergents ioniques
WO2009006438A2 (fr) 2007-06-29 2009-01-08 Epicentre Technologies Corporation Adn de copie et arn sens
GB0713183D0 (en) * 2007-07-06 2007-08-15 King S College London Method
KR101376359B1 (ko) * 2007-08-01 2014-03-27 다나-파버 캔서 인스티튜트 인크. 표적 서열 강화
CA2694832A1 (fr) 2007-08-06 2009-02-12 Orion Genomics Llc Nouveaux polymorphismes nucleotidiques uniques et combinaisons de nouveaux polymorphismes connus permettant de determiner l'expression specifique d'allele du gene igf2
EP2183394B1 (fr) * 2007-09-07 2012-12-12 Third Wave Technologies, Inc. Procédés et applications pour la quantification d'une cible
CA2639416C (fr) 2007-09-11 2019-12-31 F. Hoffmann-La Roche Ag Test de diagnostic pour la susceptibilite aux inhibiteurs de la b-raf kinase
US20090136942A1 (en) * 2007-09-18 2009-05-28 Oncomedx, Inc. Analysis of Extracellular RNA
EP2217720A4 (fr) 2007-11-01 2010-12-08 Univ Iowa Res Found Analyse de lieu de rca pour estimer la sensibilité à l'amd et au mpgnii
EP2071034A1 (fr) 2007-12-12 2009-06-17 bioMérieux Procédé pour le traitement d'une solution en vue d'éliminer un acide ribonucléique après amplification
JP5503552B2 (ja) 2007-12-21 2014-05-28 バイオメリュー・エスエイ メチシリン耐性黄色ブドウ球菌の検出
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
CA2715774A1 (fr) 2008-02-19 2009-08-27 Oncomethylome Sciences Sa Detection et pronostic de cancer du poumon
JP5651022B2 (ja) * 2008-03-15 2015-01-07 ホロジック，インコーポレーテッド 増幅反応中の核酸分子を分析するための組成物及び方法
EP2626435B1 (fr) 2008-03-21 2016-06-01 MDxHealth S.A. Détection et pronostic du cancer des cervicales
WO2009117698A2 (fr) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Procédés d'amplification d'arn en présence d'adn
EP2113574A1 (fr) 2008-04-28 2009-11-04 Biotype AG Substances et procédés pour un dosage de profilage à base d'ADN
JP2009268665A (ja) * 2008-05-07 2009-11-19 Canon Inc 吸入装置
WO2010011506A2 (fr) * 2008-07-23 2010-01-28 The Washington University Facteurs de risque et cible thérapeutique pour des troubles neurodégénératifs
GB0814570D0 (en) 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
WO2010028014A1 (fr) * 2008-09-03 2010-03-11 Abbott Laboratories Dosages et kits pour déterminer un tropisme du vih-1
US9127312B2 (en) 2011-02-09 2015-09-08 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
CA2679750A1 (fr) 2008-09-23 2010-03-23 Nexus Dx, Inc. Methodes de detection d'acides nucleiques dans un echantillon
EP2172563A1 (fr) 2008-09-24 2010-04-07 bioMérieux S.A. Procédé pour diminuer la dépendance envers la variation de séquence d'une cible d'acide nucléique dans une analyse d'hybridation de diagnostic
PL2963709T3 (pl) 2008-10-24 2017-11-30 Epicentre Technologies Corporation Kompozycje końców transpozonu i sposoby modyfikacji kwasów nukleinowych
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CA3161998A1 (en) 2009-02-11 2010-08-19 Caris Mpi, Inc. Molecular profiling of tumors
ES2544265T3 (es) 2009-02-11 2015-08-28 Orion Genomics, Llc Combinaciones de polimorfismos para determinar la expresión específica de alelo de IGF2
WO2010124257A2 (fr) * 2009-04-24 2010-10-28 Colby Pharmaceutical Company Procédés et coffrets pour déterminer les taux de radicaux libres d'oxygène (ofr) dans des tissus animaux et humains en tant que marqueur de pronostic pour le cancer et d'autres pathophysiologies
WO2010126913A1 (fr) 2009-04-27 2010-11-04 Gen-Probe Incorporated Procédés et coffrets pour une utilisation pour l'amplification sélective de séquences cibles
BRPI1011432A2 (pt) 2009-05-08 2019-09-24 Novartis Ag ensaios genericos para deteccao de virus influenza
WO2011032040A1 (fr) 2009-09-10 2011-03-17 Centrillion Technology Holding Corporation Procédés de séquençage ciblé
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
WO2011032088A1 (fr) 2009-09-11 2011-03-17 Arca Biopharma, Inc. Polymorphismes dans le gène pde3a
HUE038708T2 (hu) 2009-09-14 2018-11-28 Sillajen Biotherapeutics Inc Onkolitikus vaccinia vírus kombinációs rákterápia
WO2011036173A1 (fr) 2009-09-24 2011-03-31 Oncomethylome Sciences S.A. Détection et pronostic du cancer du col de l'utérus
US20160186266A1 (en) 2009-10-27 2016-06-30 Carislife Sciences, Inc. Molecular profiling for personalized medicine
KR20170098985A (ko) 2009-11-19 2017-08-30 솔리스 바이오다인 폴리펩티드 안정성 및 활성을 증가시키는 조성물 및 관련된 방법
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
CN102648295B (zh) 2009-12-07 2017-08-08 伊鲁米那股份有限公司 用于多重基因分型的多样品索引
US8501122B2 (en) 2009-12-08 2013-08-06 Affymetrix, Inc. Manufacturing and processing polymer arrays
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2011084772A2 (fr) 2009-12-21 2011-07-14 Northwestern University Troubles alléliques causés par des mutations dans trpv4
US20110151457A1 (en) 2009-12-22 2011-06-23 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
US20110165568A1 (en) 2009-12-31 2011-07-07 Life Technologies Corporation Sequences of e.coli 055:h7 genome
EP2545189B1 (fr) 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Enrichissement d'une pcr froide complète doté d'une séquence de blocage de référence
WO2011128096A1 (fr) 2010-04-16 2011-10-20 Roche Diagnostics Gmbh Marqueurs de polymorphisme destinés à prédire la réponse à un traitement par un médicament anticorps monoclonal inhibant les récepteurs de l'interleukine-6
CA2796744A1 (fr) 2010-04-17 2011-10-20 Bayer Healthcare Llc Metabolites synthetiques d'omega-carboxyaryldiphenylurees fluorosubstituees pour le traitement et la prevention de maladies et d'etats
JP5454338B2 (ja) 2010-04-28 2014-03-26 株式会社島津製作所 液滴操作によるリアルタイム核酸増幅法
CA2798123C (fr) 2010-05-05 2020-06-23 The Governing Council Of The University Of Toronto Procede de traitement d'echantillons seches utilisant un dispositif microfluidique numerique
US20130143214A1 (en) 2010-06-04 2013-06-06 Chronix Biomedical Prostate cancer associated circulating nucleic acid biomarkers
WO2012018638A2 (fr) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions de stabilisation d'adn, d'arn, de protéines dans le sang et d'autres échantillons biologiques lors du transport et du stockage à températures ambiantes
EP2598661B1 (fr) 2010-07-26 2017-09-27 Biomatrica, INC. Compositions de stabilisation d'adn, d'arn, de protéines salivaires et d'autres échantillons biologiques lors du transport et du stockage à températures ambiantes
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US8822663B2 (en) 2010-08-06 2014-09-02 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
EP2614163B1 (fr) 2010-09-07 2016-01-06 Novartis AG Tests génériques pour la détection de réovirus de mammifères
WO2012040619A2 (fr) 2010-09-24 2012-03-29 Massachusetts Eye And Ear Infirmary Méthodes et compositions pour le pronostic et/ou la détection d'une dégénérescence maculaire liée à l'âge
PT3590949T (pt) 2010-10-01 2022-08-02 Modernatx Inc Ácidos ribonucleicos contendo n1-metilpseudouracilos e suas utilizações
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
WO2012086243A1 (fr) 2010-12-21 2012-06-28 株式会社 島津製作所 Dispositif et procédé de traitement d'un composant cible dans un tube
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
WO2012103031A2 (fr) 2011-01-25 2012-08-02 Ariosa Diagnostics, Inc. Détection d'anomalies génétiques
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
CN110129436A (zh) 2011-02-02 2019-08-16 精密科学公司 Dna甲基化的数字序列分析
CA2827200A1 (fr) 2011-02-24 2012-08-30 Hill's Pet Nutrition, Inc. Compositions et procedes pour diagnostiquer et traiter des troubles renaux chez un felin
EP2678452B1 (fr) 2011-02-25 2016-02-24 Novartis AG Témoin positif interne exogène pour détection de virus
WO2012118745A1 (fr) 2011-02-28 2012-09-07 Arnold Oliphant Systèmes de dosage pour détection d'aneuploïdie et détermination du sexe
EP2691541B1 (fr) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Procédés pour éléver le niveau de séquences mutées dans un mélange de séquences sauvages et mutées
US8710200B2 (en) 2011-03-31 2014-04-29 Moderna Therapeutics, Inc. Engineered nucleic acids encoding a modified erythropoietin and their expression
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US8993341B2 (en) 2011-05-12 2015-03-31 Exact Sciences Corporation Removal of PCR inhibitors
US8808990B2 (en) 2011-05-12 2014-08-19 Exact Sciences Corporation Serial isolation of multiple DNA targets from stool
CN103649298A (zh) 2011-05-12 2014-03-19 精密科学公司 核酸的分离
EP2721175B1 (fr) 2011-06-15 2015-09-16 Hill's Pet Nutrition, Inc. Compositions et méthodes de diagnostic et de surveillance de l'hyperthyroïdie chez un félin
EP2721170A2 (fr) 2011-06-17 2014-04-23 Life Technologies Corporation Compositions et procédés de détection d'espèces et souches de cronobacter spp. et de cronobacter
CA2839896A1 (fr) 2011-06-21 2012-12-27 Alnylam Pharmaceuticals, Inc. Dosages et procedes de determination de l'activite d'un agent therapeutique chez un sujet
WO2013024175A2 (fr) 2011-08-17 2013-02-21 Technische Universität München Moyens et méthodes de diagnostic pour le diabète de type 2
DK2748332T3 (en) 2011-08-24 2018-07-16 Oxoid Ltd COMPOSITIONS AND METHODS FOR DETECTING MULTIPLE MICROORGANISMS
ES2589455T3 (es) 2011-08-31 2016-11-14 F. Hoffmann-La Roche Ag Método para predecir el riesgo de hipertensión asociado a la terapia antiangiogénesis
KR20140064923A (ko) 2011-08-31 2014-05-28 에프. 호프만-라 로슈 아게 혈관신생 억제제에 대한 반응성
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
WO2013043715A1 (fr) 2011-09-19 2013-03-28 Genentech, Inc. Polythérapies comportant des antagonistes de c-met et de b-raf
SG11201401196WA (en) 2011-10-03 2014-05-29 Moderna Therapeutics Inc Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
WO2013066641A1 (fr) 2011-10-21 2013-05-10 Chronix Biomedical Biomarqueurs des acides nucléiques circulants associés au cancer colorectal
AU2012342682A1 (en) 2011-11-23 2014-05-22 F. Hoffmann-La Roche Ag Responsiveness to angiogenesis inhibitors
US8748097B1 (en) 2011-12-02 2014-06-10 President And Fellows Of Harvard College Identification of agents for treating calcium disorders and uses thereof
AU2012352180A1 (en) 2011-12-16 2014-07-31 Moderna Therapeutics, Inc. Modified nucleoside, nucleotide, and nucleic acid compositions
WO2013095935A1 (fr) 2011-12-19 2013-06-27 Hill's Pet Nutrition, Inc. Compositions et procédés pour le diagnostic et le traitement de l'hyperthyroïdisme dans des animaux de compagnie
JP5807542B2 (ja) 2011-12-22 2015-11-10 株式会社島津製作所 対象成分を操作するためのチップデバイス及びそれを用いた方法
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
CA2858284A1 (fr) 2011-12-23 2013-06-27 Biomerieux Detection de souches variantes meca de staphylococcus aureus resistant a la methycilline
EP2798089B1 (fr) 2011-12-30 2018-05-23 Bio-rad Laboratories, Inc. Procédés et compositions pour la mise en uvre de réactions d'amplification d'acide nucléique
EP3578697B1 (fr) 2012-01-26 2024-03-06 Tecan Genomics, Inc. Compositions et procédés d'enrichissement de séquence d'acide nucléique ciblé et de génération de bibliothèques à haute efficacité
GB2501611B (en) 2012-02-21 2015-03-04 Cytonics Corp Alpha-2-macroglobulin compositions and therapeutic uses
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
EP3392347A1 (fr) 2012-04-02 2018-10-24 Life Technologies Corporation Compositions et procédés pour la détection de mycobacterium avium paratuberculosis
US10501512B2 (en) 2012-04-02 2019-12-10 Modernatx, Inc. Modified polynucleotides
EP3505176A1 (fr) 2012-04-02 2019-07-03 Moderna Therapeutics, Inc. Polynucléotides modifiés pour la production de protéines sécrétées
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
CN114854832A (zh) 2012-04-19 2022-08-05 生命技术公司 核酸扩增
EP3095879B1 (fr) 2012-04-19 2018-09-19 Life Technologies Corporation Amplification d'acides nucléiques
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
CN109082462B (zh) 2012-05-21 2022-10-28 斯克利普斯研究所 样品制备方法
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US20140005061A1 (en) 2012-06-29 2014-01-02 Life Technologies Corporation Compositions and methods for detection of multiple microorganisms
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
JP2015522293A (ja) 2012-07-19 2015-08-06 アリオサ ダイアグノスティックス インコーポレイテッドＡｒｉｏｓａ Ｄｉａｇｎｏｓｔｉｃｓ，Ｉｎｃ． 多重化連続ライゲーションに基づく遺伝子変異体の検出
WO2014015217A1 (fr) 2012-07-19 2014-01-23 Vertex Pharmaceuticals Incorporated Biomarqueurs pour patients infectés par le hcv
EP2895620B1 (fr) 2012-09-11 2017-08-02 Life Technologies Corporation Amplification d'acides nucléiques
WO2014043143A1 (fr) 2012-09-11 2014-03-20 Life Technologies Corporation Amplification d'acides nucléiques
US9212392B2 (en) 2012-09-25 2015-12-15 Exact Sciences Corporation Normalization of polymerase activity
JP6144355B2 (ja) 2012-11-26 2017-06-07 モデルナティエックス インコーポレイテッドＭｏｄｅｒｎａＴＸ，Ｉｎｃ． 化学修飾ｍＲＮＡ
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
AU2014244608B2 (en) 2013-03-14 2017-09-14 Exact Sciences Corporation Detecting neoplasm
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
EP2971130A4 (fr) 2013-03-15 2016-10-05 Nugen Technologies Inc Séquençage séquentiel
GB201308313D0 (en) 2013-05-09 2013-06-19 Medical Res Council Assay Method
DK3004388T4 (da) 2013-05-29 2023-08-28 Chronix Biomedical Påvisning og kvantificering af cellefrit dna fra donor i kredsløbet hos organtransplantatmodtagere
AU2014278730B2 (en) 2013-06-13 2020-12-10 F. Hoffmann-La Roche Ag Statistical analysis for non-invasive sex chromosome aneuploidy determination
WO2015013465A2 (fr) 2013-07-25 2015-01-29 Dch Molecular Diagnostics, Inc. Méthodes et compositions de détection d'une contamination bactérienne
EP3156797B1 (fr) 2013-08-08 2019-06-12 Institut Pasteur Corrélation de l'activité de maladie avec expansions clonales de cellules t cd8+ spécifiques 16 du papillomavirus humain chez des patients victimes d'un lichen plan buccal érosif sévère
DK3039158T3 (en) 2013-08-28 2019-03-04 Becton Dickinson Co MASSIVE PARALLEL SINGLE CELL CELL ANALYSIS
AU2014312249A1 (en) 2013-08-28 2016-03-24 Cytonics Corporation Systems, compositions, and methods for transplantation and treating conditions
CA2924669C (fr) 2013-09-20 2023-03-21 The Regents Of The University Of Michigan Compositions et methodes pour l'analyse de la radiosensibilite
US10023626B2 (en) 2013-09-30 2018-07-17 Modernatx, Inc. Polynucleotides encoding immune modulating polypeptides
WO2015051214A1 (fr) 2013-10-03 2015-04-09 Moderna Therapeutics, Inc. Polynucléotides codant pour un récepteur de lipoprotéines de faible densité
US10253358B2 (en) 2013-11-04 2019-04-09 Exact Sciences Development Company, Llc Multiple-control calibrators for DNA quantitation
US9546399B2 (en) 2013-11-13 2017-01-17 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CN105940115B (zh) 2013-11-15 2021-07-06 巴斯德研究所 恶性疟原虫青蒿素耐药性的分子标记物
WO2015089438A1 (fr) 2013-12-13 2015-06-18 Northwestern University Biomarqueurs pour les états de stress post-traumatique
WO2015095689A1 (fr) 2013-12-19 2015-06-25 Exact Sciences Corporation Molécules témoins d'acides nucléiques de synthèse
US20170009288A1 (en) 2014-02-03 2017-01-12 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
ES2812753T3 (es) 2014-03-31 2021-03-18 Mayo Found Medical Education & Res Detección de neoplasma colorectal
CA2943405A1 (fr) 2014-03-31 2015-10-08 Debiopharm International Sa Fusions de fgfr
CN113826612B (zh) 2014-06-10 2022-11-22 生物马特里卡公司 在环境温度下稳定凝血细胞
CN106572974B (zh) 2014-07-15 2021-04-23 生命技术公司 用于将分子有效递送到细胞的具有脂质聚集体的组合物和方法
US10364458B2 (en) 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
EP3177740B1 (fr) 2014-08-06 2021-01-13 Nugen Technologies, Inc. Mesures numériques à partir de séquençage ciblé
US10450562B2 (en) 2014-09-09 2019-10-22 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
WO2016073353A1 (fr) 2014-11-03 2016-05-12 Tangen Biosciences, Inc. Appareil et procédé pour la capture et la rupture de cellules, de spores ou de virus
JP7231326B2 (ja) 2014-11-10 2023-03-01 ジェネンテック， インコーポレイテッド Ｉｌ－３３媒介性障害のための治療及び診断方法
US10465249B2 (en) 2014-12-12 2019-11-05 Exact Sciences Development Company, Llc Method of characterizing ZDHHC1 DNA
US10011878B2 (en) 2014-12-12 2018-07-03 Exact Sciences Development Company Compositions and methods for performing methylation detection assays
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
CN115927612A (zh) 2015-03-27 2023-04-07 精密科学公司 检测食管疾病
EP3286338B1 (fr) 2015-04-24 2023-11-01 Atila Biosystems Incorporated Amplification avec des amorces de composition nucléotidique limitée
WO2016174130A1 (fr) 2015-04-28 2016-11-03 Université De Strasbourg Modèle de culture de cellules humaines sur la base de signatures génétiques cliniques
CN108026494A (zh) 2015-06-05 2018-05-11 米罗库鲁斯公司 限制蒸发和表面结垢的空气基质数字微流控装置和方法
EP3303548A4 (fr) 2015-06-05 2019-01-02 Miroculus Inc. Gestion de l'évaporation dans des dispositifs microfluidiques numériques
EP3307908B1 (fr) 2015-06-09 2019-09-11 Life Technologies Corporation Procédés pour le marquage moléculaire
US9789087B2 (en) 2015-08-03 2017-10-17 Thomas Jefferson University PAR4 inhibitor therapy for patients with PAR4 polymorphism
TWI710642B (zh) 2015-08-17 2020-11-21 美商庫拉腫瘤技術股份有限公司 以法呢基轉移酶（farnesyltransferase）抑制劑治療癌症病患之方法
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017070096A1 (fr) 2015-10-18 2017-04-27 Affymetrix, Inc. Génotypage multiallèles de polymorphismes mononucléotidiques et d'insertions-délétions
KR101651817B1 (ko) 2015-10-28 2016-08-29 대한민국 Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트
CA3002196A1 (fr) 2015-10-30 2017-05-04 Exact Sciences Development Company, Llc Detection par amplification multiplexee et isolement et detection d'adn issu de plasma
AU2016368265B2 (en) 2015-12-08 2021-10-28 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
WO2017100283A1 (fr) 2015-12-09 2017-06-15 Life Technologies Corporation Détection et détermination quantitative de molécules d'acide nucléique associées à une surface
US10626383B2 (en) 2016-01-15 2020-04-21 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
WO2017155858A1 (fr) 2016-03-07 2017-09-14 Insilixa, Inc. Identification de séquence d'acide nucléique à l'aide d'une extension de base unique cyclique en phase solide
WO2017184968A1 (fr) 2016-04-22 2017-10-26 Kura Oncology, Inc. Méthodes de sélection de patients atteints de cancer pour le traitement avec des inhibiteurs de farnésyltransférase
US20170321286A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by amplification of rna sequences
CN116064796A (zh) 2016-05-05 2023-05-05 精密科学公司 通过分析甲基化dna来检测肺肿瘤
CA2932910A1 (fr) 2016-06-14 2017-12-14 Entos Pharmaceuticals Inc. Methodes de diagnostic et de traitement de cancer metastatique
CN110291402B (zh) 2016-06-27 2023-09-01 朱诺治疗学股份有限公司 鉴定肽表位的方法、结合此类表位的分子和相关用途
MA45491A (fr) 2016-06-27 2019-05-01 Juno Therapeutics Inc Épitopes à restriction cmh-e, molécules de liaison et procédés et utilisations associés
AU2017299597B2 (en) 2016-07-19 2023-08-24 Exact Sciences Corporation Methylated control DNA
EP3488003B1 (fr) 2016-07-19 2023-10-25 Exact Sciences Corporation Molécules témoins d'acides nucléiques provenant d'organismes non humains
WO2018029532A1 (fr) 2016-08-10 2018-02-15 Institut Pasteur Procédés et réactifs utilisés pour la détection du paludisme à plasmodium falciparum résistant à la pipéraquine
US10596572B2 (en) 2016-08-22 2020-03-24 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
EP4293128A3 (fr) 2016-09-02 2024-03-20 Mayo Foundation for Medical Education and Research Détection d'un carcinome hépatocellulaire
WO2018071522A1 (fr) 2016-10-11 2018-04-19 Life Technologies Corporation Amplification rapide d'acides nucléiques
EP3534885B1 (fr) 2016-11-03 2021-01-20 Kura Oncology, Inc. Inhibiteurs de farnésyltransférase pour utilisation dans le traitement de cancer
WO2018111835A1 (fr) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions et procédés pour le codage par code-barres moléculaire de molécules d'adn avant l'enrichissement des mutations et/ou la détection des mutations
JP2020515815A (ja) 2016-12-28 2020-05-28 ミロキュラス インコーポレイテッド デジタルマイクロ流体デバイスおよび方法
WO2018129368A2 (fr) 2017-01-06 2018-07-12 Editas Medicine, Inc. Procédés d'évaluation de la coupure par les nucléases
JP7289264B2 (ja) 2017-01-27 2023-06-09 エグザクト サイエンシーズ コーポレーション メチル化ｄｎａの分析による結腸腫瘍の検出
CN114796218A (zh) 2017-02-21 2022-07-29 库拉肿瘤学公司 使用法尼基转移酶抑制剂治疗癌症的方法
US9956215B1 (en) 2017-02-21 2018-05-01 Kura Oncology, Inc. Methods of treating cancer with farnesyltransferase inhibitors
US11285486B2 (en) 2017-03-24 2022-03-29 Gen-Probe Incorporated Cover assembly and related methods of use
WO2018187476A1 (fr) 2017-04-04 2018-10-11 Miroculus Inc. Appareils microfluidiques numériques et procédés de manipulation et de traitement de gouttelettes encapsulées
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
CN110914448A (zh) 2017-06-02 2020-03-24 昂飞股份有限公司 使用差异性标记的等位基因特异性探针分析混合样品的基于阵列的方法
US11441174B2 (en) 2017-06-02 2022-09-13 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
WO2018231818A1 (fr) 2017-06-16 2018-12-20 Life Technologies Corporation Acides nucléiques de régulation, et compositions, kits et utilisations de ces derniers
WO2019002178A1 (fr) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab Mutants d'adn polymérases thermophiles
CA3155871A1 (en) 2017-07-10 2019-01-17 Gen-Probe Incorporated Analytical systems and methods for nucleic acid amplification using sample assigning parameters
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
US11413617B2 (en) 2017-07-24 2022-08-16 Miroculus Inc. Digital microfluidics systems and methods with integrated plasma collection device
US10806730B2 (en) 2017-08-07 2020-10-20 Kura Oncology, Inc. Methods of treating cancer with farnesyltransferase inhibitors
KR20200051601A (ko) 2017-08-07 2020-05-13 쿠라 온콜로지, 인크. 파르네실전달효소 억제제를 이용하여 암을 치료하는 방법
CN115582155A (zh) 2017-09-01 2023-01-10 米罗库鲁斯公司 数字微流控设备及其使用方法
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2019089982A1 (fr) 2017-11-01 2019-05-09 Juno Therapeutics, Inc. Procédé d'évaluation de l'activité de récepteurs antigéniques de recombinaison
MX2020004507A (es) 2017-11-09 2020-08-13 Alnylam Pharmaceuticals Inc Ensayos y métodos para determinar la expresión del gen de quimiotaxina 2 derivada de leucocitos (lect2).
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
WO2019130347A1 (fr) 2017-12-26 2019-07-04 Sree Chitra Tirunal Institute For Medical Sciences And Technology Amorces pour l'amplification isotherme du gène mpt64 de mycobacterium tuberculosis et processus associé
AU2019212931A1 (en) 2018-01-29 2020-08-27 Gen-Probe Incorporated Analytical systems and methods
MA51741A (fr) 2018-02-09 2021-05-19 Hoffmann La Roche Procédés thérapeutiques et de diagnostic pour des maladies inflammatoires médiées par des mastocytes
EP3775272A4 (fr) 2018-04-02 2021-12-29 Progenity, Inc. Procédés, systèmes et compositions de comptage de molécules d'acide nucléique
CA3105684A1 (fr) 2018-07-10 2020-01-16 Gen-Probe Incorporated Procedes et systemes de detection et de quantification d'acides nucleiques
US11408030B2 (en) 2018-09-10 2022-08-09 Andy Madrid Test for detecting Alzheimer's disease
SG11202104296TA (en) 2018-11-01 2021-05-28 Kura Oncology Inc Methods of treating cancer with farnesyltransferase inhibitors
CA3117768A1 (fr) 2018-11-28 2020-06-04 Keygene N.V. Enrichissement cible par protection d'endonuclease
IL311084A (en) 2018-11-30 2024-04-01 Caris Mpi Inc New generation molecular characterization
WO2020142347A2 (fr) 2018-12-31 2020-07-09 Gen-Probe Incorporated Systèmes et procédés de remplissage de cartouches à puits multiples avec des réactifs solides
EP3927840A1 (fr) 2019-02-21 2021-12-29 Keygene N.V. Génotypage de polyploïdes
US20220142983A1 (en) 2019-03-01 2022-05-12 Kura Oncology, Inc. Methods of treating cancer with farnesyltransferase inhibitors
CN113924041A (zh) 2019-03-14 2022-01-11 因斯利克萨公司 基于时间门控的荧光检测的方法和系统
SG11202110472WA (en) 2019-03-29 2021-10-28 Kura Oncology Inc Methods of treating squamous cell carcinomas with farnesyltransferase inhibitors
TW202102218A (zh) 2019-04-01 2021-01-16 美商庫拉腫瘤技術股份有限公司 以法呢基(farnesyl)轉移酶抑制劑治療癌症的方法
EP3947718A4 (fr) 2019-04-02 2022-12-21 Enumera Molecular, Inc. Procédés, systèmes et compositions de comptage de molécules d'acide nucléique
EP3953041A4 (fr) 2019-04-08 2023-01-25 Miroculus Inc. Appareils microfluidiques numériques à cartouches multiples et procédés d'utilisation
BR112021020779A8 (pt) 2019-04-17 2022-01-25 Igenomix S L Métodos aperfeiçoados para diagnóstico precoce de leiomiomas e leiomiossarcomas uterinos
US20220305001A1 (en) 2019-05-02 2022-09-29 Kura Oncology, Inc. Methods of treating acute myeloid leukemia with farnesyltransferase inhibitors
CA3176699A1 (fr) 2019-05-03 2020-11-12 Gen-Probe Incorporated Systeme de transport de receptacle pour systeme analytique
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
EP4051811A4 (fr) 2019-10-31 2023-12-06 Mayo Foundation for Medical Education and Research Détection du cancer de l'ovaire
EP4069865A4 (fr) 2019-12-02 2023-12-20 Caris MPI, Inc. Prédicteur de réponse au platine dans une approche pan-cancer
WO2021116371A1 (fr) 2019-12-12 2021-06-17 Keygene N.V. Manipulation d'acide nucléique semi-solide
AU2020407850A1 (en) 2019-12-20 2022-06-30 Keygene N.V. NGS library preparation using covalently closed nucleic acid molecule ends
US20230110203A1 (en) 2020-03-13 2023-04-13 Medimmune Limited Therapeutic methods for the treatment of subjects with risk alelles in il33
US11761031B2 (en) 2020-05-28 2023-09-19 The Hong Kong University Of Science And Technology Method for real time monitoring of nucleic acid amplicons mediated by loop oligonucleotide probes
CA3188230A1 (fr) 2020-08-19 2022-02-24 John B. Kisiel Detection de lymphome non hodgkinien
JP2023543424A (ja) 2020-09-21 2023-10-16 ビオラ・セラピューティクス・インコーポレイテッド 無細胞ｄｎａを単離するための組成物および方法
JP2023543602A (ja) 2020-10-06 2023-10-17 キージーン ナムローゼ フェンノートシャップ 標的化された配列付加
US20230393163A1 (en) 2020-10-21 2023-12-07 Gen-Probe Incorporated Fluid container management system
US20240002904A1 (en) 2020-11-24 2024-01-04 Keygene N.V. Targeted enrichment using nanopore selective sequencing
EP4251750A1 (fr) 2020-11-25 2023-10-04 Koninklijke Nederlandse Akademie van Wetenschappen Profilage ribosomique dans des cellules individuelles
EP4320443A1 (fr) 2021-04-06 2024-02-14 BPGbio, Inc. Marqueurs protéiques pour le pronostic de la progression du cancer du sein
WO2023011660A1 (fr) 2021-08-06 2023-02-09 Sansure Biotech Inc. Compositions pour liquéfier un échantillon biologique visqueux, produit combiné, agents liquéfiants, kits associés et procédés et application associés
US11857961B2 (en) 2022-01-12 2024-01-02 Miroculus Inc. Sequencing by synthesis using mechanical compression
WO2023240201A1 (fr) 2022-06-08 2023-12-14 Larimar Therapeutics, Inc. Marqueurs sensibles à la frataxine pour surveiller la progression et le traitement du syndrome de leigh
WO2024015331A1 (fr) 2022-07-12 2024-01-18 Genentech, Inc. Méthodes thérapeutiques et diagnostiques pour la sclérose en plaques

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988010315A1 (fr) * 1987-06-19 1988-12-29 Siska Diagnostics, Inc. Systemes d'amplification/detection d'acides nucleiques a base de transcription
EP0310229A1 (fr) * 1987-07-31 1989-04-05 The Board Of Trustees Of The Leland Stanford Junior University Amplification sélective de séquences de cible oligonucléotide
WO1989006700A1 (fr) * 1988-01-21 1989-07-27 Genentech, Inc. Amplification et detection de sequences d'acides nucleiques
EP0201184B1 (fr) * 1985-03-28 1992-12-16 F. Hoffmann-La Roche Ag Procédé pour l'amplification des séquences d'acide nucléique
EP0200362B1 (fr) * 1985-03-28 1993-01-20 F. Hoffmann-La Roche Ag Procédé pour l'amplification, la détection et/ou le clonage des séquences d'acide nucléique

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
JP2517241B2 (ja) * 1986-08-19 1996-07-24 郁男 山科 遺伝子
EP0272098A3 (fr) * 1986-12-15 1990-06-06 City Of Hope National Medical Center Procédé d'amplification et de détection de séquences ARN
DE3726934A1 (de) * 1987-08-13 1989-02-23 Merck Patent Gmbh Verfahren zum nachweis von nukleinsaeure-sequenzen
CA1340807C (fr) * 1988-02-24 1999-11-02 Lawrence T. Malek Procede d'amplification d'une sequence d'acide nucleique
AU4829690A (en) * 1988-12-16 1990-07-10 Siska Diagnostics, Inc. Self-sustained, sequence replication system

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0201184B1 (fr) * 1985-03-28 1992-12-16 F. Hoffmann-La Roche Ag Procédé pour l'amplification des séquences d'acide nucléique
EP0200362B1 (fr) * 1985-03-28 1993-01-20 F. Hoffmann-La Roche Ag Procédé pour l'amplification, la détection et/ou le clonage des séquences d'acide nucléique
WO1988010315A1 (fr) * 1987-06-19 1988-12-29 Siska Diagnostics, Inc. Systemes d'amplification/detection d'acides nucleiques a base de transcription
EP0310229A1 (fr) * 1987-07-31 1989-04-05 The Board Of Trustees Of The Leland Stanford Junior University Amplification sélective de séquences de cible oligonucléotide
WO1989006700A1 (fr) * 1988-01-21 1989-07-27 Genentech, Inc. Amplification et detection de sequences d'acides nucleiques

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Biochemicals for Molecular Biology, Boehringer Mannheim 1987, pp. 107-108 *
Enzyme Nomenclature, Academic Press, 1984, pp. 302-303 *
Science 239, 491, 1988 *

Cited By (249)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0778352A1 (fr) * 1988-12-16 1997-06-11 Siska Diagnostics, Inc. Essai par réplication auto-entretenue de séquences
US7083922B2 (en) 1989-07-11 2006-08-01 Gen-Probe Incorporated Detection of HIV
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
US5766849A (en) * 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
EP0731174A3 (fr) * 1989-07-11 1998-03-25 Gen-Probe Incorporated Méthodes pour l'amplification de séquences d'acide nucléique
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
US7132235B2 (en) 1990-02-16 2006-11-07 Orchid Cellmark Inc. Reagent kit for determining specific nucleotide variations
US7026117B2 (en) 1990-02-16 2006-04-11 Orchid Biosciences, Inc. Method for determining specific nucleotide variations
US7585512B1 (en) 1990-05-08 2009-09-08 Thomas Jefferson University Composition and method of using tumor cells
EP0479376A1 (fr) 1990-10-05 1992-04-08 Akzo Nobel N.V. Peptides agissants immunochimiquemeur avec des anticorps contre le virus d'hépatite non-A, non-B
WO1992008800A1 (fr) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Amplification d'acides nucleiques par replication bi-enzymatique auto-entretenue de sequences
EP0525882B1 (fr) * 1991-08-02 2004-01-14 bioMerieux B.V. Quantification d'acides nucléiques
US5643730A (en) * 1991-09-23 1997-07-01 Pfizer Inc. Process for detecting specific mRNA and DNA in cells
US7501252B2 (en) 1992-01-10 2009-03-10 Life Technologies Corporation Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
WO1993015228A1 (fr) * 1992-01-29 1993-08-05 Hitachi Chemical Co., Ltd. Support d'immobilisation de polynucleotide
EP0566198A3 (fr) * 1992-04-13 1994-08-03 Enichem Spa
US5554516A (en) * 1992-05-06 1996-09-10 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US5888729A (en) * 1992-05-06 1999-03-30 Gen-Probe Incorporated Oligonucleotide probes and methods for detecting Streptococcus pneumoniae
ES2056028A1 (es) * 1993-02-18 1994-09-16 Inia Procedimiento para la seleccion de oligonucleotidos para la amplificacion especifica de genomas altamente variables.
EP0656425A1 (fr) * 1993-07-23 1995-06-07 Gen-Probe Incorporated Procédé pour améliorer l'amplification des acides nucléiques
WO1995003430A1 (fr) * 1993-07-23 1995-02-02 Gen-Probe Incorporated Procede d'amelioration de l'amplification de l'acide nucleique
USRE39007E1 (en) * 1994-03-16 2006-03-07 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
USRE38960E1 (en) * 1994-03-16 2006-01-31 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
WO1995027721A1 (fr) * 1994-04-07 1995-10-19 Akzo Nobel N.V. Compositions lyophilisees comprenant de l'arn
US6031150A (en) * 1994-04-13 2000-02-29 Baylor College Of Medicine Sulfonylurea receptor trangenic rodents
US6054313A (en) * 1994-04-13 2000-04-25 Baylor College Of Medicine Nucleic acid and amino acid sequences for mammalian sulfonylurea receptor
EP0688873A2 (fr) 1994-06-23 1995-12-27 Bayer Ag Test DNA rapide pour la détection de staphylococcus aureus résistant à la chinolone dans du matériel clinique
EP0776376B1 (fr) * 1994-07-15 2001-11-21 Akzo Nobel N.V. Utilisation d'arn polymerase pour ameliorer un procede d'amplification d'acide nucleique
WO1996006191A3 (fr) * 1994-08-18 1996-03-14 Akzo Nobel Nv Amorces et sondes destinees a l'amplification et a la detection de l'acide nucleique du cytomegalovirus
EP0710724A2 (fr) 1994-10-06 1996-05-08 Akzo Nobel N.V. Antigènes de Toxoplasma gondii
EP0713922A1 (fr) 1994-10-28 1996-05-29 BIO MERIEUX, Société anonyme Oligonucléotide utilisable comme amorce dans une méthode d'amplification basée sur une réplication avec déplacement de brin
US5789169A (en) * 1994-11-03 1998-08-04 Regents Of The University Of California Methods for the diagnosis of glaucoma
US6248867B1 (en) 1994-11-03 2001-06-19 Univ California Trabecular meshwork induced glucocorticoid response (TIGR) fusion protein
US6150161A (en) * 1994-11-03 2000-11-21 The Regents Of The University Of California Methods for the diagnosis of glaucoma
EP0803578A3 (fr) * 1996-04-26 1999-01-13 Toyo Boseki Kabushiki Kaisha Coffret pour l'amplification et la détection des acides nucléiques du cytomegalovirus (CMV) utilisant une séquence d'acide nucléique ss2.7
WO1998006736A1 (fr) 1996-08-14 1998-02-19 Life Technologies, Inc. Compositions stables pour l'amplification et le sequençage d'acides nucleiques
EP2264045A1 (fr) 1996-08-14 2010-12-22 Life Technologies Corporation Composition pour l'amplification et pour la séquenciation de l'ADN
US6132954A (en) * 1996-08-20 2000-10-17 Baylor College Of Medicine Methods of screening for agents that delay a cell cycle and compositions comprising era and an analogue of wild-type era
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
US6905858B2 (en) 1997-01-02 2005-06-14 Invitrogen Corporation Nucleic acid-free thermostable enzymes and methods of production thereof
US8062848B2 (en) 1997-01-02 2011-11-22 Life Technologies Corporation Nucleic acid-free thermostable enzymes and methods of production thereof
US6475724B1 (en) 1997-01-28 2002-11-05 The Regents Of The University Of California Nucleic acids, kits, and methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
US6171788B1 (en) 1997-01-28 2001-01-09 The Regents Of The University Of California Methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
US7138511B1 (en) 1997-01-28 2006-11-21 The Regents Of The University Of California Nucleic acids, kits and methods for the diagnosis, prognosis and treatment of glaucoma and related disorders
US8735066B2 (en) 1997-01-30 2014-05-27 Board Of Regents, The University Of Texas System Tumor suppressor designated TS10Q23.3
EP2366712A2 (fr) 1997-01-30 2011-09-21 The Board Of Regents, The University Of Texas System Suppresseur de tumeur désigné par TS10Q23.3
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US7501237B2 (en) 1997-02-07 2009-03-10 Life Technologies Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US7192579B2 (en) 1997-02-27 2007-03-20 Baylor College Of Medicine Methods of gene therapy using nucleic acid sequences for ATP-binding cassette transporter
US7189511B2 (en) 1997-02-27 2007-03-13 Baylor College Of Medicine Methods of screening and diagnostics using ATP-binding cassette transporter
US7141420B2 (en) 1997-02-27 2006-11-28 University Of Utah Research Foundation Nucleic acid and amino acid sequences for ATP-binding cassette transporter and methods of screening for agents that modify ATP-binding cassette transporter
US8129353B2 (en) 1997-02-27 2012-03-06 Baylor College Of Medicine Methods of gene therapy using nucleic acid sequences for ATP-binding cassette transporter
EP2184289A1 (fr) 1997-04-22 2010-05-12 Life Technologies Corporation Procédés de production de transcriptases inverses ASLV composées de plusieurs subunités
EP2251347A2 (fr) 1997-04-22 2010-11-17 Life Technologies Corporation Procédés de production de transcriptases inverses ASLV composées de plusieurs sous-unités
EP2336367A1 (fr) 1997-08-08 2011-06-22 bioMerieux B.V. Séquences nucléotidiques pouvant être utilisées comme amorces et comme sondes au cours de l'amplification et la détection de tous les sous-types du VIH-1
US6413761B2 (en) 1997-09-17 2002-07-02 Toyota Jidosha Kabushiki Kaisha Decaprenyl diphosphate synthetase gene
US6410280B1 (en) 1997-09-17 2002-06-25 Toyota Jidosha Kabushiki Kaisha Decaprenyl diphosphate synthetase gene
US6225097B1 (en) 1997-09-17 2001-05-01 Toyota Jidosha Kabushiki Kaisha Decaprenyl diphosphate synthetase gene
US7105318B2 (en) 1997-11-04 2006-09-12 Roche Diagnostics Gmbh Specific and sensitive nucleic acid detection method
EP1801220A2 (fr) 1997-12-18 2007-06-27 Monsanto Technology, LLC Plantes transgéniques résistant aux insectes et procédés permettant d'améliorer l'activité de delta-toxine contre des insectes
WO1999046400A1 (fr) 1998-03-13 1999-09-16 Life Technologies, Inc. Compositions et procedes d'augmentation de la synthese des molecules d'acide nucleique
US6261773B1 (en) 1998-03-17 2001-07-17 Toyo Boseki Kabushiki Kaisha Reagent for nucleic acid amplification and process for nucleic acid amplification
US7029861B1 (en) 1998-09-15 2006-04-18 Board Of Regents, The University Of Texas System LPS-response gene compositions and methods
US7306804B2 (en) 1998-11-17 2007-12-11 Board Of Regents, The University Of Texas System HIV-specific T-cell induction
EP1734134A2 (fr) 1999-02-09 2006-12-20 Laboratory Corporation of America Holdings, Inc. Nouveau gène spécifique à la prostate servant au diagnostic, au prognostic et au traitment du cancer de la prostate
US7993830B2 (en) 1999-02-09 2011-08-09 Laboratory Corporation Of America Holdings Prostate-specific gene for diagnosis, prognosis and management of prostate cancer
US6808883B1 (en) 1999-04-15 2004-10-26 Bayer Ag Automatable rapid test for detection of cancer, based on telomerase (hTC) mRNA with specific primers and probes
US7342111B2 (en) 1999-04-30 2008-03-11 University Of Florida Research Foundation, Inc. Adeno-associated virus-delivered ribozyme compositions and methods of use
WO2000073764A2 (fr) 1999-06-01 2000-12-07 Baylor College Of Medicine Compositions et methodes pour l'utilisation therapeutique d'une sequence associee au gene atonal dans le traitement de la surdite, de l'osteoarthrite et d'une proliferation cellulaire anormale
EP2325198A1 (fr) 1999-06-01 2011-05-25 Baylor College Of Medicine Compositions et procédés pour l'utilisation thérapeutique d'une séquence à association atonale
US7537886B1 (en) 1999-06-22 2009-05-26 Life Technologies Corporation Primers and methods for the detection and discrimination of nucleic acids
US8043816B2 (en) 1999-07-02 2011-10-25 Life Technologies Corporation Compositions and methods for temperature-dependent nucleic acid synthesis
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
EP1978098A2 (fr) 1999-12-10 2008-10-08 Invitrogen Corporation Utilisation de sites à recombinaison multiples avec une spécificité unique de clonage recombinatoire
EP2210948A2 (fr) 1999-12-10 2010-07-28 Life Technologies Corporation Utilisation de sites à recombinaison multiples avec une spécificité unique de clonage recombinatoire
WO2001064959A1 (fr) * 2000-03-02 2001-09-07 Akzo Nobel N.V. Detection d'arn du virus de l'hepatite b
WO2001066705A1 (fr) * 2000-03-07 2001-09-13 Akzo Nobel N.V. Mutants d'arn polymerases presentant une stabilite thermique amelioree
US7507567B2 (en) 2000-03-07 2009-03-24 Biomerieus B.V. RNA polymerase mutants with increased thermostability
EP2192128A2 (fr) 2000-04-21 2010-06-02 Corixa Corporation Composés et méthodes pour le traitement et le diagnostique des infections de Chlamydia
EP3093341A1 (fr) 2000-05-26 2016-11-16 Life Technologies Corporation Transcriptases inverses thermostables et utilisations
EP2295551A1 (fr) 2000-05-26 2011-03-16 Life Technologies Corporation Transcriptases inverses thermostables et utilisations
WO2002004511A2 (fr) 2000-07-10 2002-01-17 Board Of Regents, The University Of Texas System Genes du chromosome 3p21.3 constituant des suppresseurs de tumeurs
CN1322143C (zh) * 2000-12-04 2007-06-20 普里马根公司 检测线粒体核酸与核基因核酸比率确定药物活性和/或副作用的方法
WO2002046470A3 (fr) * 2000-12-04 2004-01-15 Primagen Holding Bv Analyse d'organites cellulaires endosymbiontes et composes pouvant etre identifies par cette analyse
EP1229130A2 (fr) * 2000-12-04 2002-08-07 PrimaGen B.V. Tests basés sur les acides nucléiques des organelles cellulaires endosymbiontiques et les composés identifiables
EP1229130A3 (fr) * 2000-12-04 2003-10-22 PrimaGen Holding B.V. Tests basés sur les acides nucléiques des organelles cellulaires endosymbiontiques et les composés identifiables
EP2105496A1 (fr) 2000-12-08 2009-09-30 Life Technologies Corporation Méthodes et compositions destinées à la synthèse de molécules d'acide nucléique à l'aide de sites de reconnaissance multiples
EP2105502A1 (fr) 2000-12-12 2009-09-30 Corixa Corporation Composés et procédés de thérapie et de diagnostic du cancer du poumon
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
WO2002095002A2 (fr) 2001-05-22 2002-11-28 University Of Chicago Arn polymerase dependant de l'adn a brin unique de virion n4
US8722866B2 (en) 2001-08-01 2014-05-13 The United States Of America, As Represented By The Department Of Veterans Affairs Isoform-selective inhibitors and activators of PDE3 cyclic nucleotide phosphodiesterases
EP1908851A2 (fr) 2001-09-19 2008-04-09 Intergenetics Incorporated Analyse génétique pour la stratification du risque de cancer
EP2135960A1 (fr) 2001-09-19 2009-12-23 Intergenetics Incorporated Analyse génétique pour la stratification du risque de cancer par détermination du profile allelique des génes VDR-ApaI et CYP11b2
US8460867B2 (en) 2001-12-10 2013-06-11 Novartis Ag Methods of treating psychosis and schizophrenia based on polymorphisms in the CNTF gene
WO2003053220A2 (fr) 2001-12-17 2003-07-03 Corixa Corporation Compositions et procedes applicables a la therapie et au diagnostic pour la maladie intestinale inflammatoire
EP1975231A1 (fr) 2002-01-22 2008-10-01 Corixa Corporation Compositions et procédés de détection, diagnostic et de thérapie de malignancies hématologiques
WO2005019409A2 (fr) 2002-07-15 2005-03-03 Board Of Regents, The University Of Texas System Criblage combinatoire de banques de proteines par expression periplasmique
EP2044948A1 (fr) 2002-08-12 2009-04-08 Jennerex Biotherapeutics ULC Procédés et compositions concernant des poxvirus et le cancer
EP2269618A1 (fr) 2002-08-12 2011-01-05 Jennerex Biotherapeutics ULC Virus de vaccinia oncolytique pour usage en combinaison aven une chémiothérapie pour le traitement du cancer
EP2269619A1 (fr) 2002-08-12 2011-01-05 Jennerex Biotherapeutics ULC Procédés et compositions concernant des poxvirus et le cancer
EP2325303A2 (fr) 2002-09-13 2011-05-25 Life Technologies Corporation Transcriptases inverses thermostables et leurs utilisations
EP2940028A1 (fr) 2003-01-06 2015-11-04 Corixa Corporation Composes d'aminoalkyle glucosaminide phosphate et leur utilisation
WO2004062599A2 (fr) 2003-01-06 2004-07-29 Corixa Corporation Composes d'aminoalkyle glucosaminide phosphate et leur utilisation
EP2145955A2 (fr) 2003-01-29 2010-01-20 454 Corporation Amplification d'acides nucléiques par emulsion de billes
EP2261372A2 (fr) 2003-01-29 2010-12-15 454 Life Sciences Corporation Procédé d'amplification et de séquençage d'acides nucléiques
US8748102B2 (en) 2003-01-29 2014-06-10 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
EP1997889A2 (fr) 2003-01-29 2008-12-03 454 Corporation Procédé de préparation de bibliothèques ADN à simple brin
US8012690B2 (en) 2003-01-29 2011-09-06 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
EP2159285A2 (fr) 2003-01-29 2010-03-03 454 Life Sciences Corporation Procédés d'amplification et de séquençage d'acides nucléiques
EP2390352A1 (fr) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systemes et procedes pour accroitre la production de proteines et de lait chez des bovins laitiers
EP2298891A1 (fr) 2003-07-25 2011-03-23 Ambion, Inc. Procédés et compositions pour isoler des petites molécules d'ARN
EP3366771A1 (fr) 2003-07-25 2018-08-29 Life Technologies Corporation Procédé pour isoler des petites molécules d'arn
EP2902490A1 (fr) 2003-07-25 2015-08-05 Life Technologies Corporation Procédé pour isoler des petites molécules d'ARN
EP2295569A1 (fr) 2003-07-25 2011-03-16 Ambion, Inc. Procédés et compositions pour isoler des petites molécules de RNA
US7449292B2 (en) 2003-09-12 2008-11-11 University Of Cincinnati Methods for predicting relative efficacy of a beta blocker therapy based on a B1-adrenergic receptor polymorphism
WO2005112544A2 (fr) 2004-02-19 2005-12-01 The Governors Of The University Of Alberta Polymorphismes du promoteur de la leptine et utilisations correspondantes
EP2527446A1 (fr) 2004-06-03 2012-11-28 Athlomics Pty Ltd Agents et procédés pour diagnostiquer le stress
EP2527447A1 (fr) 2004-06-03 2012-11-28 Athlomics Pty Ltd Agents et procédés pour diagnostiquer le stress
EP2270034A2 (fr) 2004-06-03 2011-01-05 Athlomics Pty Ltd Agents et procédé permettant le diagnostic de stress
EP2940027A1 (fr) 2004-07-08 2015-11-04 Corixa Corporation Composes d'iaminoalkylglucosaminide phosphate et leur utilisation
US8916603B2 (en) 2004-09-14 2014-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods for treatment with bucindolol based on genetic targeting
US8093286B2 (en) 2004-09-14 2012-01-10 The Regents Of The University Of Colorado, A Body Corporate Methods for treatment with bucindolol based on genetic targeting
EP2246444A1 (fr) 2004-09-14 2010-11-03 The Regents of the University of Colorado, A Body Corporate Procédé de traitement faisant intervenir du bucindolol fondé sur le ciblage génétique
US8080578B2 (en) 2004-09-14 2011-12-20 The Regents Of The University Of Colorado, A Body Corporate Methods for treatment with bucindolol based on genetic targeting
US9505846B2 (en) 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
EP3056566A1 (fr) 2005-01-28 2016-08-17 Life Technologies Corporation Inhibiteurs multi-composants de polymerases d'acides nucleiques et methodes associees
EP2365079A1 (fr) 2005-03-05 2011-09-14 Seegene, Inc. Procédés utilisant un oligonucléotide à double spécificité et oligonucléotide à double spécificité
EP2365078A1 (fr) 2005-03-05 2011-09-14 Seegene, Inc. Procédés utilisant un oligonucléotide à double spécificité et oligonucléotide à double spécificité
WO2006122354A1 (fr) 2005-05-17 2006-11-23 Ozgene Pty Ltd Systeme de clonage sequentiel
EP2476761A2 (fr) 2005-07-07 2012-07-18 Athlomics Pty Ltd Gènes de marqueur de polynucléotide et leur expression pour le diagnostic de l'endotoxine
US7494788B2 (en) 2005-07-11 2009-02-24 Molecular Kinetics, Inc. Entropic bristle domain sequences and their use in recombinant protein production
EP2662456A1 (fr) 2005-08-02 2013-11-13 Focus Diagnostics, Inc. Procédés et compositions pour détecter le virus BK
WO2007016275A2 (fr) 2005-08-02 2007-02-08 Focus Diagnostics, Inc. Procedes et compositions pour detecter le virus bk
EP2319941A2 (fr) 2005-10-21 2011-05-11 GeneNews Inc. Procédé et appareil pour corréler des niveaux de produits biomarqueurs avec une maladie
EP2368868A1 (fr) 2005-10-28 2011-09-28 Praecis Pharmaceuticals Inc. Procédé d'identification de composés intéressants utilisant des bibliothèques codées
EP2299267A2 (fr) 2005-11-16 2011-03-23 Novartis AG Biomarqueurs pour un traitement par des anticorps anti-NogoA de lésions de la moelle épinière
US8222023B2 (en) 2006-03-15 2012-07-17 Micronics, Inc. Integrated nucleic acid assays
US8772017B2 (en) 2006-03-15 2014-07-08 Micronics, Inc. Integrated nucleic acid assays
EP2455491A2 (fr) 2006-05-25 2012-05-23 Monsanto Technology LLC Procédé pour identifier des locus de traits quantitatifs résistants aux maladies dans le soja et compositions correspondantes
EP2455490A2 (fr) 2006-05-25 2012-05-23 Monsanto Technology LLC Procédé pour identifier des locus de traits quantitatifs résistants aux maladies dans le soja et compositions correspondantes
EP2455489A2 (fr) 2006-05-25 2012-05-23 Monsanto Technology LLC Procédé pour identifier des locus de traits quantitatifs résistants aux maladies dans le soja et compositions correspondantes
EP2442108A1 (fr) 2006-07-14 2012-04-18 The Regents of the University of California Biomarqueurs du cancer et procédé d'utilisation correspondant
EP2450710A2 (fr) 2006-07-14 2012-05-09 The Regents of the University of California Biomarqueurs du cancer et procédé d'utilisation correspondant
EP2442109A1 (fr) 2006-07-14 2012-04-18 The Regents of the University of California Biomarqueurs du cancer et procédé d'utilisation correspondant
EP3796002A1 (fr) 2006-07-14 2021-03-24 The Regents of The University of California Biomarqueurs du cancer et leurs procédés d'utilisation
EP2839837A1 (fr) 2006-09-15 2015-02-25 Ottawa Hospital Research Institute Rhabdovirus Oncolytique Farmington
WO2008067547A2 (fr) 2006-11-30 2008-06-05 Research Development Foundation Bibliothèques d'immunoglobulines améliorées
WO2008092866A1 (fr) 2007-01-29 2008-08-07 Scientific Institute Of Public Health (Iph) Détection d'événements dans des plantes transgéniques
EP2623605A1 (fr) 2007-04-09 2013-08-07 University of Florida Research Foundation, Inc. Compositions de vecteur RAAV comprenant des protéines de capside à tyrosine modifié et procédés d'utilisation
EP3492596A1 (fr) 2007-04-09 2019-06-05 University of Florida Research Foundation, Inc. Compositions de vecteur raav comprenant des protéines de capside à tyrosine modifié et procédés d'utilisation
WO2008137475A2 (fr) 2007-05-01 2008-11-13 Research Development Foundation Banques d'immunoglobulines fc
US8568730B2 (en) 2009-04-22 2013-10-29 Indiana University Research & Technology Corporation Compositions for use in the treatment of chronic obstructive pulmonary diseases and asthma
EP2789689A1 (fr) 2009-06-29 2014-10-15 Luminex Corporation Amorces chimères avec des conformations en épingle à cheveux et leurs procédés d'utilisation
WO2011008530A2 (fr) 2009-06-29 2011-01-20 Luminex Corporation Amorces chimériques avec conformations en épingle à cheveux et procédés d'utilisation de celles-ci
EP3636759A1 (fr) 2009-07-17 2020-04-15 BioAtla LLC Sélection simultanée et intégrée et évolution des performances d'anticorps/de protéines et de l'expression dans des hôtes de production
US10047357B2 (en) 2009-07-17 2018-08-14 Bioatla, Llc Simultaneous, integrated selection and evolution of antibody/protein performance and expression in production hosts
EP3042957A1 (fr) 2009-07-17 2016-07-13 Bioatla LLC Evolution et sélection simultanée of intégrée d'anticorps/performance de protéines et expression dans des hôtes de production
EP2907873A1 (fr) 2009-07-17 2015-08-19 Bioatla LLC Évolution et sélection simultanée et intégrée d'anticorps/performance de protéines et expression dans des hôtes de production
US8859467B2 (en) 2009-07-17 2014-10-14 Bioatla, Llc Simultaneous, integrated selection and evolution of antibody/protein performance and expression in production hosts
US10920216B2 (en) 2009-07-17 2021-02-16 Bioatla, Inc. Simultaneous, integrated selection and evolution of antibody/protein performance and expression in production hosts
US10106788B2 (en) 2009-07-17 2018-10-23 Bioatla, Llc Simultaneous, integrated selection and evolution of antibody/protein performance and expression in production hosts
US9409983B2 (en) 2009-07-23 2016-08-09 The Board Of Trustess Of The University Of Illinois Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases
US8614062B1 (en) 2009-07-24 2013-12-24 University Of South Florida RNA-based system and method to differentiate seafood
US9896664B2 (en) 2009-12-10 2018-02-20 Turnstone Limited Partnership Oncolytic rhabdovirus
WO2011079273A2 (fr) 2009-12-23 2011-06-30 Arca Biopharma, Inc. Procédés et compositions pour maladies et affections cardiovasculaires
US9895692B2 (en) 2010-01-29 2018-02-20 Micronics, Inc. Sample-to-answer microfluidic cartridge
WO2011127933A1 (fr) 2010-04-16 2011-10-20 Nuevolution A/S Complexes bifonctionnels et procédés de fabrication et d'utilisation de tels complexes
EP3540059A1 (fr) 2010-04-16 2019-09-18 Nuevolution A/S Complexes bifonctionnels et procédés de fabrication et d'utilisation de tels complexes
WO2011133933A2 (fr) 2010-04-23 2011-10-27 University Of Florida Research Foundation, Inc. Compositions de guanylate cyclase raav et méthodes de traitement de l'amaurose-1 congénitale de leber (lca1)
EP4056700A1 (fr) 2010-04-23 2022-09-14 University of Florida Research Foundation, Inc. Compositions raav-guanylate cyclase et procédés de traitement de l'amaurose-1 congénitale de leber (lca1)
EP3486320A1 (fr) 2010-04-23 2019-05-22 University of Florida Research Foundation, Inc. Compositions raav-guanylate cyclase et procédés de traitement de l'amaurose-1 congénitale de leber (lca1)
WO2011139721A1 (fr) 2010-04-27 2011-11-10 The Regents Of The University Of California Biomarqueurs de cancer et leurs procédés d'utilisation
EP3508854A1 (fr) 2010-04-27 2019-07-10 The Regents of The University of California Biomarqueurs du cancer et leurs procédés d'utilisation
WO2011145777A1 (fr) 2010-05-20 2011-11-24 광주과학기술원 Composition pharmaceutique comprenant un inhibiteur de hif-2α comme ingrédient actif pour la prévention ou le traitement de l'arthrite
WO2011145795A1 (fr) 2010-05-20 2011-11-24 광주과학기술원 Souris transgénique hif-2α spécifique du cartilage comme modèle animal pour l'arthrite, et son utilisation
EP2913402A1 (fr) 2010-07-16 2015-09-02 Tocagen Inc. Détection de rétrovirus
WO2012090073A2 (fr) 2010-12-30 2012-07-05 The Netherlands Cancer Institute Procédés et compositions pour prédire la sensibilité à la chimiothérapie
US9919047B2 (en) 2011-01-04 2018-03-20 Sillajen, Inc. Generation of antibodies to tumor antigens and generation of tumor specific complement dependent cytotoxicity by administration of oncolytic vaccinia virus
WO2012109133A1 (fr) 2011-02-07 2012-08-16 Research Development Foundation Polypeptides fc modifiés d'immunoglobuline
WO2012158238A2 (fr) 2011-02-28 2012-11-22 University Of Iowa Research Foundation Changements d'hormone antimullérienne dans la grossesse et prédiction d'évolution indésirable de grossesse et du sexe
WO2012120377A2 (fr) 2011-03-08 2012-09-13 King Abdullah University Of Science And Technology Biomarqueur moléculaire destiné à la détection précoce du cancer des ovaires
US9057107B2 (en) 2011-03-08 2015-06-16 King Abdullah University Of Science And Technology Molecular biomarker set for early detection of ovarian cancer
US9914974B2 (en) 2011-03-08 2018-03-13 King Abdullah University Of Science And Technology Molecular biomarker set for early detection of ovarian cancer
WO2012138789A2 (fr) 2011-04-04 2012-10-11 Netherlands Cancer Institute Procédés et compositions pour prédire une résistance à un traitement anti-cancéreux
WO2012138783A2 (fr) 2011-04-04 2012-10-11 Netherlands Cancer Institute Procédés et compositions pour prédire une résistance à un traitement anti-cancéreux
US8841432B2 (en) 2011-04-21 2014-09-23 Snu R&Db Foundation Shuttle vectors for mycobacteria-escherichia coli and uses thereof
WO2012149038A1 (fr) 2011-04-25 2012-11-01 Advanced Bioscience Laboratories, Inc. Protéines tronquées d'enveloppe (env) du vih, procédés et compositions associés à celles-ci
US10772951B2 (en) 2011-06-08 2020-09-15 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
US11654192B2 (en) 2011-06-08 2023-05-23 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
US10040853B2 (en) 2011-09-09 2018-08-07 Fred Hutchinson Cancer Research Center Methods and compositions involving NKG2D inhibitors and cancer
WO2013045505A1 (fr) 2011-09-28 2013-04-04 Novartis Ag Biomarqueurs pour traitement combiné par des agents sraa
US9540670B2 (en) 2011-10-06 2017-01-10 bioMérieux Mutated T7 RNA Polymerases
WO2013071233A1 (fr) 2011-11-10 2013-05-16 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Procédés de détection d'agents infectieux et nouveau virus détecté par ces procédés
WO2013074676A2 (fr) 2011-11-14 2013-05-23 The General Hospital Corporation Dosages et procédés pour la sélection d'un schéma thérapeutique pour un sujet atteint d'une dépression
WO2013093629A2 (fr) 2011-12-20 2013-06-27 Netherlands Cancer Institute Vaccins modulaires, procédés et compositions qui y sont liés
WO2013096798A2 (fr) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Amplification d'une séquence provenant d'un acide ribonucléique
WO2013116698A2 (fr) 2012-02-02 2013-08-08 Invenra, Inc. Crible haut débit pour des polypeptides biologiquement actifs
WO2013144249A1 (fr) 2012-03-29 2013-10-03 Novartis Ag Produit pharmaceutique à des fins diagnostiques
EP3524691A1 (fr) 2012-12-07 2019-08-14 SuppreMol GmbH Stratification et traitement de patients du purpura thrombocytopénique idiopathique
EP2740805A1 (fr) 2012-12-07 2014-06-11 SuppreMol GmbH Stratification et traitement de patients du purpura thrombocytopénique idiopathique
EP3249054A1 (fr) 2012-12-20 2017-11-29 Biomatrica, INC. Formulations et procédés pour stabiliser des réactifs pcr
US10518262B2 (en) 2012-12-21 2019-12-31 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
US11181105B2 (en) 2012-12-21 2021-11-23 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
US10065186B2 (en) 2012-12-21 2018-09-04 Micronics, Inc. Fluidic circuits and related manufacturing methods
US10436713B2 (en) 2012-12-21 2019-10-08 Micronics, Inc. Portable fluorescence detection system and microassay cartridge
US10125373B2 (en) 2013-01-22 2018-11-13 Arizona Board Of Regents On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
US10660947B2 (en) 2013-02-21 2020-05-26 Turnstone Limited Partnership Vaccine composition
US10363293B2 (en) 2013-02-21 2019-07-30 Turnstone Limited Partnership Vaccine composition
US10646557B2 (en) 2013-02-21 2020-05-12 Turnstone Limited Partnership Vaccine composition
WO2014148817A1 (fr) 2013-03-21 2014-09-25 주식회사 현일바이오 Procédé de détection sélective pour mycobacterium tuberculosis et des mycobactéries non tuberculeuses et kit utilisant celui-ci
WO2014171698A1 (fr) 2013-04-18 2014-10-23 사회복지법인 삼성생명공익재단 Méthode de diagnostic de la dystrophie myotonique de type 1
US10087440B2 (en) 2013-05-07 2018-10-02 Micronics, Inc. Device for preparation and analysis of nucleic acids
US10190153B2 (en) 2013-05-07 2019-01-29 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
US11016108B2 (en) 2013-05-07 2021-05-25 Perkinelmer Health Sciences, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
US10386377B2 (en) 2013-05-07 2019-08-20 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
WO2014183023A1 (fr) 2013-05-09 2014-11-13 Trustees Of Boston University Utilisation de la plexine-a4 comme biomarqueur et cible thérapeutique pour la maladie d'alzheimer
US11585810B2 (en) 2013-10-03 2023-02-21 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
US10393739B2 (en) 2013-10-03 2019-08-27 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
WO2015105888A1 (fr) 2014-01-07 2015-07-16 Bioatla, Llc Orthologues ciblant les protéines
EP3447493A1 (fr) 2014-01-07 2019-02-27 Bioatla, LLC Orthologues ciblant les protéines
US11293929B2 (en) 2014-01-07 2022-04-05 Bioatla, Inc. Proteins targeting orthologs
WO2015112767A2 (fr) 2014-01-22 2015-07-30 Life Technologies Corporation Nouvelles rétrotranscriptases pour une utilisation dans la synthèse d'acide nucléique à haute température
WO2015191916A1 (fr) 2014-06-11 2015-12-17 Micronics, Inc. Cartouches microfluidiques et appareil à témoins de dosage intégrés pour l'analyse d'acides nucléiques
WO2016086227A2 (fr) 2014-11-26 2016-06-02 The Regents Of The University Of California Compositions thérapeutiques comprenant des facteurs de transcription et leurs procédés de préparation et d'utilisation
US11543411B2 (en) 2014-12-05 2023-01-03 Prelude Corporation DCIS recurrence and invasive breast cancer
EP4242329A2 (fr) 2014-12-08 2023-09-13 Berg LLC Utilisation de marqueurs comprenant de la filamine a dans le diagnostic et le traitement du cancer de la prostate
US10172879B2 (en) 2015-01-21 2019-01-08 D.R.Nano Co., Ltd Nanocomplexes for co-delivering a drug and siRNA and uses thereof
WO2016130516A1 (fr) 2015-02-09 2016-08-18 Research Development Foundation Polypeptides ayant un domaine fc d'immunoglobuline modifié manifestant une activation du complément améliorée
WO2017015075A1 (fr) 2015-07-17 2017-01-26 President And Fellows Of Harvard College Procédés d'amplification de séquences d'acides nucléiques
WO2017040380A2 (fr) 2015-08-28 2017-03-09 Research Development Foundation Variants de fc d'anticorps modifiés
US11339421B2 (en) 2016-02-23 2022-05-24 Industry Foundation Of Chonnam National University Leukemia diagnostic kit targeting prohibitin gene and diagnostic method using same
WO2017214068A1 (fr) 2016-06-05 2017-12-14 Berg Llc Systèmes et procédés de stratification de patient et identification de biomarqueurs potentiels
WO2018128516A1 (fr) 2017-01-09 2018-07-12 연세대학교 산학협력단 Composition pharmaceutique pour un traitement de précancer buccal et procédé de prédiction ou de diagnostic d'un précancer buccal ou d'un cancer buccal
WO2018140606A1 (fr) 2017-01-26 2018-08-02 Oklahoma Medical Research Foundation Biomarqueurs de l'activité, de l'intensité et de l'éruption de la maladie du lupus érythémateux disséminé
WO2018219997A1 (fr) 2017-05-30 2018-12-06 Roche Diagnostics Gmbh Catalyseurs pour inverser des produits d'addition de formaldéhyde et des réticulations
WO2019017680A2 (fr) 2017-07-19 2019-01-24 국민대학교 산학협력단 Micro-arn utilisé en tant que biomarqueur de la maladie de parkinson et kit diagnostique le mettant en oeuvre
US11447772B2 (en) 2017-07-19 2022-09-20 Kookmin University Industry Academy Cooperation Foundation miRNA as biomarker for Parkinson's disease and diagnostic kit using same
EP4275684A1 (fr) 2018-05-25 2023-11-15 Arca Biopharma, Inc. Procédés et compositions impliquant du bucindolol pour le traitement de la fibrillation auriculaire
US11821900B2 (en) 2018-09-14 2023-11-21 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
WO2020089841A1 (fr) 2018-10-31 2020-05-07 Garcia Joe G N Biomarqueurs et méthodes d'utilisation de ces biomarqueurs pour détecter une lésion pulmonaire radio-induite
WO2020223576A1 (fr) 2019-04-30 2020-11-05 Chondrial Therapeutics, Inc. Marqueurs sensibles à la frataxine pour déterminer l'efficacité d'une thérapie de remplacement de frataxine
WO2021246820A1 (fr) 2020-06-05 2021-12-09 주식회사 씨젠 Kit de transport d'échantillon pour la détection d'agents pathogènes respiratoires et procédés de détection d'agents pathogènes respiratoires à l'aide de celui-ci
WO2022047359A1 (fr) 2020-08-31 2022-03-03 Berg Llc Biomarqueurs protéiques du cancer du pancréas
WO2022216846A1 (fr) 2021-04-06 2022-10-13 Berg Llc Marqueurs protéiques pour le cancer du sein du type positif aux récepteurs des oestrogènes (er) et du type négatif aux récepteurs des oestrogènes (er)
WO2022216841A1 (fr) 2021-04-06 2022-10-13 Berg Llc Marqueurs protéiques pour un cancer du sein positif au récepteur des œstrogènes (er) de type luminal a (la) et de type luminal b1 (lb1)
WO2022265965A1 (fr) 2021-06-14 2022-12-22 10X Genomics, Inc. Variants de la transcriptase inverse pour une performance améliorée
WO2023196937A1 (fr) 2022-04-06 2023-10-12 Larimar Therapeutics, Inc. Marqueurs sensibles à la frataxine pour surveiller une thérapie de remplacement de frataxine
WO2023230531A1 (fr) 2022-05-24 2023-11-30 Lunglife Ai, Inc. Procédés de détection de cellules génétiquement anormales circulantes
WO2023239940A1 (fr) 2022-06-10 2023-12-14 Research Development Foundation Variants de fc igg1 sélectifs de fcriib modifiés et leurs utilisations

Also Published As

Publication number Publication date
WO1991002814A1 (fr) 1991-03-07
DE3850093T2 (de) 1994-11-03
KR890013184A (ko) 1989-09-21
ES2053648T3 (es) 1994-08-01
CA1340807C (fr) 1999-11-02
EP0329822B1 (fr) 1994-06-08
US5409818A (en) 1995-04-25
KR960015744B1 (ko) 1996-11-20
KR920702866A (ko) 1992-10-28
EP0329822A3 (en) 1990-08-01
ATE106948T1 (de) 1994-06-15
DE3850093D1 (de) 1994-07-14

Similar Documents

Publication Publication Date Title
EP0329822B1 (fr) Procédé d'amplification d'acides nucléiques
US6063603A (en) Nucleic acid amplification process
EP0487628B1 (fr) Procede d'amplification ameliore d'acides nucleiques
US5466586A (en) Method for the synthesis of ribonucleic acid (RNA)
KR100392551B1 (ko) 핵산 증폭방법을 개선시키기 위한 rna폴리머라제의 용도
US5437990A (en) Selective amplification of target polynucleotide sequences
US5169766A (en) Amplification of nucleic acid molecules
US5665545A (en) Terminal repeat amplification method
JP2843586B2 (ja) 転写に基づいた核酸増幅／検出系
FI99143C (fi) Itseään ylläpitävä sekvenssin replikaatiosysteemi
US5869251A (en) Use of primers containing nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
US6063604A (en) Target nucleic acid sequence amplification
JPH02503054A (ja) 核酸配列の増幅および検出
KR20010041371A (ko) 핵산의 비특이적 증폭방법
IE892698A1 (en) Nucleic acid amplification process
CA2223527A1 (fr) Amplification lineaire liee d'acides nucleiques
JPH0576399A (ja) イン ビトロで複製可能な核酸の製造方法

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE CH DE ES FR GB GR IT LI LU NL SE

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): AT BE CH DE ES FR GB GR IT LI LU NL SE

17P Request for examination filed

Effective date: 19901127

17Q First examination report despatched

Effective date: 19910502

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE CH DE ES FR GB GR IT LI LU NL SE

REF Corresponds to:

Ref document number: 106948

Country of ref document: AT

Date of ref document: 19940615

Kind code of ref document: T

REF Corresponds to:

Ref document number: 3850093

Country of ref document: DE

Date of ref document: 19940714

RAP2 Party data changed (patent owner data changed or rights of a patent transferred)

Owner name: CANGENE CORPORATION

ET Fr: translation filed
REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2053648

Country of ref document: ES

Kind code of ref document: T3

REG Reference to a national code

Ref country code: GR

Ref legal event code: FG4A

Free format text: 3012159

ITF It: translation for a ep patent filed

Owner name: SAIC BREVETTI S.R.L.

EAL Se: european patent in force in sweden

Ref document number: 88113948.9

PLBI Opposition filed

Free format text: ORIGINAL CODE: 0009260

26 Opposition filed

Opponent name: GEN- PROBE INC.

Effective date: 19950308

NLR1 Nl: opposition has been filed with the epo

Opponent name: GEN-PROBE INC.

PLBF Reply of patent proprietor to notice(s) of opposition

Free format text: ORIGINAL CODE: EPIDOS OBSO

PLBL Opposition procedure terminated

Free format text: ORIGINAL CODE: EPIDOS OPPC

PLBM Termination of opposition procedure: date of legal effect published

Free format text: ORIGINAL CODE: 0009276

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: OPPOSITION PROCEDURE CLOSED

27C Opposition proceedings terminated

Effective date: 19970807

NLR2 Nl: decision of opposition
REG Reference to a national code

Ref country code: GB

Ref legal event code: IF02

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: LU

Payment date: 20070809

Year of fee payment: 20

Ref country code: DE

Payment date: 20070809

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: ES

Payment date: 20070824

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: AT

Payment date: 20070718

Year of fee payment: 20

Ref country code: CH

Payment date: 20070813

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20070810

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20070816

Year of fee payment: 20

Ref country code: BE

Payment date: 20070911

Year of fee payment: 20

Ref country code: SE

Payment date: 20070717

Year of fee payment: 20

Ref country code: NL

Payment date: 20070719

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20070831

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GR

Payment date: 20070730

Year of fee payment: 20

BE20 Be: patent expired

Owner name: *CANGENE CORP.

Effective date: 20080826

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

REG Reference to a national code

Ref country code: GB

Ref legal event code: PE20

Expiry date: 20080825

EUG Se: european patent has lapsed
PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20080826

NLV7 Nl: ceased due to reaching the maximum lifetime of a patent

Effective date: 20080826

REG Reference to a national code

Ref country code: ES

Ref legal event code: FD2A

Effective date: 20080827

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20080825

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20080827