EA200501772A1 - Способы измерения экспрессии генов из фиксированного и залитого в парафин образца ткани - Google Patents

Способы измерения экспрессии генов из фиксированного и залитого в парафин образца ткани

Info

Publication number
EA200501772A1
EA200501772A1 EA200501772A EA200501772A EA200501772A1 EA 200501772 A1 EA200501772 A1 EA 200501772A1 EA 200501772 A EA200501772 A EA 200501772A EA 200501772 A EA200501772 A EA 200501772A EA 200501772 A1 EA200501772 A1 EA 200501772A1
Authority
EA
Eurasian Patent Office
Prior art keywords
fixed
methods
parulin
genes
expression
Prior art date
Application number
EA200501772A
Other languages
English (en)
Other versions
EA008832B1 (ru
Inventor
Кэтлин Даненберг
Питер Даненберг
Стивен Свенсон
Original Assignee
Юниверсити Оф Саутерн Калифорния
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Юниверсити Оф Саутерн Калифорния filed Critical Юниверсити Оф Саутерн Калифорния
Publication of EA200501772A1 publication Critical patent/EA200501772A1/ru
Publication of EA008832B1 publication Critical patent/EA008832B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Описаны быстрые, надежные и простые способы выделения РНК из фиксированных и залитых в парафин образцов тканей. РНК, очищенная таким способом, может быть использована для контроля уровней экспрессии генов. Образцом ткани может быть опухоль или другая патологическая ткань
EA200501772A 1999-12-20 2000-10-31 Способы измерения экспрессии генов из фиксированного и залитого в парафин образца ткани EA008832B1 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/469,338 US6248535B1 (en) 1999-12-20 1999-12-20 Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens

Publications (2)

Publication Number Publication Date
EA200501772A1 true EA200501772A1 (ru) 2006-10-27
EA008832B1 EA008832B1 (ru) 2007-08-31

Family

ID=23863395

Family Applications (2)

Application Number Title Priority Date Filing Date
EA200501772A EA008832B1 (ru) 1999-12-20 2000-10-31 Способы измерения экспрессии генов из фиксированного и залитого в парафин образца ткани
EA200200698A EA006827B1 (ru) 1999-12-20 2000-10-31 Способ выделения рнк из залитых в парафин образцов ткани

Family Applications After (1)

Application Number Title Priority Date Filing Date
EA200200698A EA006827B1 (ru) 1999-12-20 2000-10-31 Способ выделения рнк из залитых в парафин образцов ткани

Country Status (20)

Country Link
US (8) US6248535B1 (ru)
EP (3) EP1743939B1 (ru)
JP (1) JP2003525033A (ru)
KR (1) KR100723328B1 (ru)
CN (2) CN1891836B (ru)
AR (1) AR027036A1 (ru)
AT (2) ATE349518T1 (ru)
AU (2) AU784565C (ru)
BR (1) BR0016569A (ru)
CA (1) CA2394618C (ru)
DE (1) DE60032634T2 (ru)
DK (1) DK1743939T3 (ru)
EA (2) EA008832B1 (ru)
ES (1) ES2373149T3 (ru)
HK (2) HK1055446A1 (ru)
IL (2) IL150116A0 (ru)
MX (1) MXPA02006145A (ru)
NO (1) NO330802B1 (ru)
TW (1) TWI277652B (ru)
WO (1) WO2001046402A1 (ru)

Families Citing this family (208)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010023070A (ko) * 1997-08-20 2001-03-26 더 유니버시티 오브 마이애미 고품질의 연속적인 재료 처리, 조직 고정-탈수-지방제거-함침 방법
US6793890B2 (en) 1997-08-20 2004-09-21 The University Of Miami Rapid tissue processor
US6960439B2 (en) 1999-06-28 2005-11-01 Source Precision Medicine, Inc. Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US20060166227A1 (en) * 2000-06-20 2006-07-27 Stephen Kingsmore Protein expression profiling
US6518416B1 (en) 2000-12-01 2003-02-11 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US6602670B2 (en) * 2000-12-01 2003-08-05 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
NZ546251A (en) * 2000-12-01 2008-01-31 Response Genetics Inc Method of determining epidermal growth factor receptor gene expression in a parafin embedded tiasue sample and correlation of levels thereof with survival rates
US20020142328A1 (en) 2000-12-01 2002-10-03 Danenberg Kathleen D. Method of determining a chemotherapeutic regimen by assaying gene expression in primary tumors
US7049059B2 (en) * 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
US6956111B2 (en) * 2001-03-02 2005-10-18 Response Genetics, Inc. Method of determining dihydropyrimidine dehydrogenase gene expression
US6686155B2 (en) 2001-06-14 2004-02-03 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on glutathione-S-transferase pi expression
US20040091857A1 (en) * 2001-07-20 2004-05-13 Nallur Girish N. Gene expression profiling
ES2607978T3 (es) * 2001-10-12 2017-04-05 Becton, Dickinson And Company Aparato para almacenar muestras biológicas
ES2636974T3 (es) * 2001-10-12 2017-10-10 Becton, Dickinson And Company Aparato de tipo cesta para el transporte de muestras biológicas
JP5236856B2 (ja) 2001-11-09 2013-07-17 ライフ テクノロジーズ コーポレーション 遺伝子発現プロファイルを用いる病気の同定、観測及び治療及び生物学的状態の同定
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
DK2799555T3 (en) 2002-03-13 2017-05-22 Genomic Health Inc Gene Expression Profiling in Tumor Tissue Biopsies
EP2385137A1 (en) 2002-07-31 2011-11-09 University of Southern California Polymorphisms for predicting disease and treatment outcome
CN100374576C (zh) * 2002-10-11 2008-03-12 阿克丘勒斯生物科学股份有限公司 来自ffpe样品的基因表达分布图
US7364846B2 (en) * 2002-10-11 2008-04-29 Molecular Devices Corporation Gene expression profiling from FFPE samples
US8039218B2 (en) * 2002-11-14 2011-10-18 John Wayne Cancer Institute Detection of cancer cells in body fluids
EP2363500A1 (en) 2002-11-14 2011-09-07 John Wayne Cancer Institute Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimaker quantitative RT-PCR
AU2003295598B2 (en) * 2002-11-15 2009-12-24 Genomic Health, Inc. Gene expression profiling of EGFR positive cancer
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US20040231909A1 (en) 2003-01-15 2004-11-25 Tai-Yang Luh Motorized vehicle having forward and backward differential structure
PT1597391E (pt) * 2003-02-20 2008-12-19 Genomic Health Inc Utilização de arn intrónico para medir a expressão genética
ES2428941T3 (es) 2003-03-10 2013-11-12 Expression Pathology, Inc. Preparación líquida de tejidos a partir de muestras biológicas, tejidos y células procesadas histopatológicamente
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
CA2527321A1 (en) * 2003-05-30 2004-12-23 Genomic Health, Inc. Gene expression markers for response to egfr inhibitor drugs
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
EP3470535B1 (en) 2003-06-24 2020-04-01 Genomic Health, Inc. Prediction of likelihood of cancer recurrence
ES2651849T3 (es) 2003-07-10 2018-01-30 Genomic Health, Inc. Algoritmo del perfil de expresión y test para el pronóstico del cáncer
US8062897B2 (en) 2003-07-21 2011-11-22 Aureon Laboratories, Inc. Diagnostic histopathology using multiplex gene expression FISH
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
JP4871722B2 (ja) * 2003-07-25 2012-02-08 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー 固定試料からｒｎａを調製するための方法および組成物
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
EP2311994A1 (en) * 2003-08-01 2011-04-20 Life Technologies Corporation Compositions and methods for preparing short RNA molecules and other nucleic acids
CA2542656A1 (en) * 2003-10-16 2005-05-06 Genomic Health, Inc. Qrt-pcr assay system for gene expression profiling
DE602004023104D1 (de) * 2003-10-24 2009-10-22 Univ Miami Vereinfachte gewebeverarbeitung
MXPA06005424A (es) 2003-11-13 2007-01-25 Univ Georgia Res Found Metodos para caracterizar virus de enfermedad bursal infecciosa.
AU2004309396B2 (en) * 2003-12-23 2010-05-13 Genomic Health, Inc. Universal amplification of fragmented RNA
EP2947160B1 (en) 2004-04-09 2017-07-12 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
US7794932B2 (en) * 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
WO2005116256A2 (en) * 2004-04-16 2005-12-08 Wei-Sing Chu Device for extracting biological molecules from tissue specimens
US20050239055A1 (en) * 2004-04-22 2005-10-27 Mariani Brian D Enterovirus primers and probes
US7332597B2 (en) * 2004-06-28 2008-02-19 University Of Kentucky Research Foundation Primers and probe to identify mycobacterium tuberculosis complex
US20060115827A1 (en) * 2004-07-01 2006-06-01 University Of Southern California Genetic markers for predicting disease and treatment outcome
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
ES2564659T3 (es) 2004-09-30 2016-03-28 Epigenomics Ag Método para proveer fragmentos de ADN derivados de una muestra archivada
DE102004047772B4 (de) * 2004-09-30 2018-12-13 Infineon Technologies Ag Lateraler Halbleitertransistor
WO2006052731A2 (en) * 2004-11-05 2006-05-18 Genomic Health, Inc. Molecular indicators of breast cancer prognosis and prediction of treatment response
EP1836629B1 (en) 2004-11-05 2020-03-04 Genomic Health, Inc. Predicting response to chemotherapy using gene expression markers
US20060141481A1 (en) * 2004-12-27 2006-06-29 Mariani Brian D HSV-1 and HSV-2 primers and probes
US7541144B2 (en) * 2005-01-31 2009-06-02 Agilent Technologies, Inc. RNA labeling method
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US7449297B2 (en) * 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
EP2460886B1 (en) * 2005-05-04 2014-01-01 University of South Florida Predicting treatment response in cancer subjects
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
US20060269929A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods and kits for DNA purification on polymeric membranes at low ionic strength
US7544471B2 (en) * 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US20070054168A1 (en) * 2005-09-06 2007-03-08 Hao Chang Zinc/air cell
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP1777291A1 (en) * 2005-10-20 2007-04-25 Fundacion para la Investigacion Clinica y Molecular del Cancer de Pulmon Method for the isolation of mRNA from formalin fixed, paraffin-embedded tissue
US20070092883A1 (en) * 2005-10-26 2007-04-26 De Luwe Hoek Octrooien B.V. Methylation specific multiplex ligation-dependent probe amplification (MS-MLPA)
DE102005060738A1 (de) * 2005-12-16 2007-06-21 Qiagen Gmbh Verfahren zur Extraktion von Biomolekülen aus fixierten Geweben
CN100366632C (zh) * 2005-12-28 2008-02-06 南方医科大学 一种石蜡包埋组织提取蛋白质的方法
AU2007318115A1 (en) * 2006-10-27 2008-05-15 George Mason Intellectual Properties, Inc. Assay for metastatic colorectal cancer
WO2008135196A1 (en) * 2007-05-03 2008-11-13 Roche Diagnostics Gmbh SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR
WO2008157316A1 (en) * 2007-06-14 2008-12-24 Creighton University Improved method for rna isolation
MX2009013646A (es) * 2007-06-15 2010-03-30 Univ South Florida Metodos de diagnostico y tratamiento del cancer.
JP2010530761A (ja) 2007-06-22 2010-09-16 レスポンス ジェネティクス，インコーポレイティド 固定されたサンプルからの長い断片ｒｎａの分離方法
US9068230B2 (en) 2007-11-07 2015-06-30 Genentech, Inc. Methods and compositions for assessing responsiveness of B-cell lymphoma to treatment with anti-CD40 antibodies
WO2009127350A1 (en) * 2008-04-15 2009-10-22 Universität Bern Method for optimized isolation of rna from fixed tissue
US20090298172A1 (en) * 2008-05-28 2009-12-03 Steven Paul Wheeler Histological specimen treatment apparatus and method
DE102008029356A1 (de) * 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
EP2310527B1 (en) 2008-08-01 2015-04-29 Roche Diagnostics GmbH Improved lysis and reverse transcription for mrna quantification
CA2745431A1 (en) 2008-12-03 2010-06-10 Timothy J. O'leary Pressure-assisted molecular recovery (pamr) of biomolecules, pressure-assisted antigen retrieval (paar), and pressure-assisted tissue histology (path)
EP2193831A1 (de) 2008-12-05 2010-06-09 Qiagen GmbH Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe
DE102008061714A1 (de) 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
US8846878B1 (en) 2009-01-23 2014-09-30 Cubrc Corporation Method and device for isolating a protein sample
EP2210936A1 (en) * 2009-01-27 2010-07-28 Curetis AG Processing and analysis of viscous liquid biological samples
AU2010236168B2 (en) * 2009-04-18 2015-08-13 Genentech, Inc. Methods for assessing responsiveness of B-cell lymphoma to treatment with anti-CD40 antibodies
WO2010123625A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms predict clinical outcome in patients with cancer
DE102009031434B4 (de) * 2009-07-01 2015-03-05 Axagarius Gmbh & Co. Kg Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit hierfür
UA115219C2 (uk) 2009-09-23 2017-10-10 Даніско Юес Інк. Глікозилгідролазні ферменти і їх застосування
US8329360B2 (en) 2009-12-04 2012-12-11 Taiwan Semiconductor Manufacturing Company, Ltd. Method and apparatus of providing overlay
BR112012018407A8 (pt) 2009-12-23 2018-11-27 Danisco Us Inc método para melhorar a eficiência de reações de fermentação e sacarificação simultâneas
AU2011203450B2 (en) * 2010-01-04 2016-01-07 Qiagen Gaithersburg, Inc. Methods, compositions, and kits for recovery of nucleic acids or proteins from fixed tissue samples
US9422542B2 (en) 2010-02-26 2016-08-23 Qiagen Gmbh Process for parallel isolation and/or purification of RNA and DNA
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
EP3399036B1 (en) 2010-04-08 2023-01-25 QIAGEN GmbH Chromatographic device and method for isolating and purifying nucleic acids
CN102812118B (zh) 2010-04-08 2016-01-20 恰根有限公司 分离和纯化核酸的方法
AU2011240653A1 (en) 2010-04-12 2012-11-01 Response Genetics, Inc. KRAS primers and probes
EP2388324A1 (de) * 2010-05-20 2011-11-23 Siemens Healthcare Diagnostics Inc. Kontrollmaterial für Nukleinsäureteste
EP2395082A1 (en) * 2010-06-14 2011-12-14 QIAGEN GmbH Extraction of nucleic acids from wax-embedded samples
CN103261873A (zh) * 2010-10-12 2013-08-21 生命科技股份有限公司 制备用于ffpe的质量对照材料的方法
KR101420094B1 (ko) * 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
TWI425977B (zh) 2010-11-24 2014-02-11 Ind Tech Res Inst 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組
EP2468862A1 (en) * 2010-12-23 2012-06-27 Qiagen GmbH Method and kit for processing wax-embedded biological samples
CN102146112B (zh) * 2011-01-25 2013-07-10 天根生化科技（北京）有限公司 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法
WO2012117294A2 (en) * 2011-02-28 2012-09-07 Dako Denmark A/S Two phase immiscible system for the pretreatment of embedded biological samples
CN102181431B (zh) * 2011-03-11 2012-12-26 大连医科大学 从石蜡包埋组织中提取总rna的方法
RU2013146245A (ru) 2011-03-17 2015-04-27 ДАНИСКО ЮЭс ИНК. Способ уменьшения вязкости в процессе осахаривания
CA2830673C (en) 2011-03-28 2019-11-12 Hui Wang Telomere length measurement in formalin-fixed, paraffin embedded (ffpe) samples by quantitative pcr
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
FR2982278A1 (fr) 2011-11-03 2013-05-10 Assist Publ Hopitaux De Paris Procede de classification d'echantillons de tissus fixes et inclus en paraffine
US9416357B2 (en) 2011-11-16 2016-08-16 University Of Georgia Research Foundation, Inc. Method for isolating total RNA from cells
WO2013074885A1 (en) 2011-11-17 2013-05-23 Rheonix, Inc. Microfluidic apparatus, method, and applications
CN103571824B (zh) 2012-08-09 2016-04-13 财团法人工业技术研究院 用于分离核酸的组合物及方法
US10233440B2 (en) 2012-09-28 2019-03-19 Cepheid Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
US9783820B2 (en) 2012-10-15 2017-10-10 Pioneer Hi-Bred International, Inc. Methods and compositions to enhance activity of Cry endotoxins
US10968446B2 (en) 2012-11-01 2021-04-06 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
EP2730653A1 (en) 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
US9200327B2 (en) 2012-11-30 2015-12-01 Geron Corporation Diagnostic markers for treating cell proliferative disorders with telomerase inhibitors
WO2014153254A2 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International Inc. Compositions and methods to control insect pests
RU2015143825A (ru) 2013-03-15 2017-04-26 Пайонир Хай-Бред Интернэшнл, Инк. Полипептиды phi-4 и способы их применения
KR101748867B1 (ko) * 2013-04-19 2017-06-20 주식회사 젠큐릭스 조기 유방암 예후 예측 진단용 자동화 시스템
EP3013984B1 (en) 2013-06-25 2023-03-22 Prognosys Biosciences, Inc. Methods for determining spatial patterns of biological targets in a sample
US10006045B2 (en) 2013-08-16 2018-06-26 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3692786B1 (en) 2013-09-13 2022-11-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA2938979C (en) 2014-02-07 2024-02-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX2016010187A (es) 2014-02-07 2017-07-11 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
WO2016000237A1 (en) 2014-07-03 2016-01-07 Pioneer Overseas Corporation Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes
US20170247719A1 (en) 2014-09-17 2017-08-31 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
UA126192C2 (uk) 2014-10-16 2022-08-31 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок та спосіб його застосування
DE102014118532B4 (de) 2014-12-12 2017-03-23 Axagarius Gmbh & Co. Kg Vorrichtung mit einer Filterschicht und Verfahren zur Gewinnung von Nukleinsäuren aus Formalin-fixierten und in Paraffin eingebetteten Proben
WO2016109799A2 (en) * 2014-12-31 2016-07-07 The Rockefeller University Method of rna isolation from clinical samples
US10876132B2 (en) 2015-03-11 2020-12-29 Pioneer Hi-Bred International, Inc. Insecticidal combinations of PIP-72 and methods of use
EP3530752B1 (en) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10365189B2 (en) 2015-05-07 2019-07-30 Steven Wheeler Histological specimen treatment
RU2017144238A (ru) 2015-05-19 2019-06-19 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
EA033170B1 (ru) * 2015-05-21 2019-09-30 Государственное Учреждение Образования "Белорусская Медицинская Академия Последипломного Образования" (Гуо "Белмапо") Способ определения экспрессии гена gstm1 в образцах из парафин-фиксированных срезов опухолевой ткани молочной железы
MX2017016119A (es) 2015-06-16 2018-03-07 Pioneer Hi Bred Int Composiciones y metodos para controlar plagas de insectos.
CN108602729B (zh) 2015-07-13 2022-07-05 皮沃特生物公司 改良植物性状的方法及组合物
JP7099951B2 (ja) 2015-07-24 2022-07-12 セファイド 組織試料からのｄｎａ及びｒｎａ抽出のための組成物及び方法
BR122023016256B8 (pt) 2015-08-06 2024-04-30 E I Du Pont De Nemours And Company Polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, método de obtenção de uma planta transgênica ou célula de planta transgênica, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas e uso do polipeptídeo
CN108513584A (zh) 2015-08-28 2018-09-07 先锋国际良种公司 苍白杆菌介导的植物转化
AU2016336328A1 (en) 2015-10-05 2018-04-19 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
KR101749419B1 (ko) 2015-10-15 2017-07-03 주식회사 어큐진 파라핀포매된 샘플로부터 dna를 추출하는 방법
CA3004504A1 (en) 2015-11-06 2017-05-11 Ventana Medical Systems, Inc. Tissue homogenisation for representative diagnostics
US20180325119A1 (en) 2015-12-18 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3442994A4 (en) 2016-04-14 2019-11-27 Pioneer Hi-Bred International, Inc. INSECTICIDES POLYPEPTIDES WITH IMPROVED EFFICIENCY SPECTRUM AND USES THEREOF
WO2017184673A1 (en) 2016-04-19 2017-10-26 Pioneer Hi-Bred International, Inc. Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof
MX2018013249A (es) 2016-05-04 2019-02-13 Pioneer Hi Bred Int Proteinas insecticidas y metodos para sus usos.
CA3022858A1 (en) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
RU2019101793A (ru) 2016-06-24 2020-07-24 Пайонир Хай-Бред Интернэшнл, Инк. Регуляторные элементы растений и способы их применения
MX2018015906A (es) 2016-07-01 2019-04-04 Pioneer Hi Bred Int Proteinas insecticidas de plantas y metodos para sus usos.
US20210292778A1 (en) 2016-07-12 2021-09-23 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US11021716B2 (en) 2016-11-01 2021-06-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018111551A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN110050070B (zh) 2016-12-22 2023-12-22 文塔纳医疗系统公司 用于组织样品的全自动化核酸提取方法
EP3558004A1 (en) 2016-12-22 2019-10-30 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
KR20190104056A (ko) 2017-01-12 2019-09-05 피벗 바이오, 인크. 식물 형질 개선을 위한 방법 및 조성물
WO2018140214A1 (en) 2017-01-24 2018-08-02 Pioneer Hi-Bred International, Inc. Nematicidal protein from pseudomonas
CA3052794A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
BR112019024827A2 (pt) 2017-05-26 2020-06-16 Pioneer Hi-Bred International, Inc. Construto de dna, planta transgênica ou progênie da mesma, composição e método para controlar uma população de pragas de insetos
CN107271239A (zh) * 2017-07-06 2017-10-20 浙江大学 一种溶解石蜡组织切片的方法及应用
US20200165626A1 (en) 2017-10-13 2020-05-28 Pioneer Hi-Bred International, Inc. Virus-induced gene silencing technology for insect control in maize
WO2019165245A1 (en) 2018-02-22 2019-08-29 Zymergen Inc. Method for creating a genomic library enriched for bacillus and identification of novel cry toxins
KR20200127180A (ko) 2018-03-02 2020-11-10 지머젠 인코포레이티드 살충 단백질 발견 플랫폼 및 이로부터 발견된 살충 단백질
US20210002657A1 (en) 2018-03-02 2021-01-07 Pioneer Hi-Bred International, Inc. Plant health assay
BR112020023800A2 (pt) 2018-05-22 2021-02-23 Pioneer Hi-Bred International, Inc. elementos reguladores de planta e métodos de uso dos mesmos
WO2020006064A2 (en) 2018-06-27 2020-01-02 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
BR112021003797A2 (pt) 2018-08-29 2021-05-25 Pioneer Hi-Bred International, Inc. proteínas inseticidas e métodos para seu uso
EP3894591A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Imaging system hardware
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN110376036A (zh) * 2019-07-30 2019-10-25 河南赛诺特生物技术有限公司 一种肿瘤细胞系免疫组化对照品的制备方法及肿瘤细胞系免疫组化对照品
CN110361242B (zh) * 2019-08-14 2022-01-18 武汉赛维尔生物科技有限公司 一种用于眼球组织的固定液以及眼球组织制片的预处理方法
WO2021076346A1 (en) 2019-10-18 2021-04-22 Pioneer Hi-Bred International, Inc. Maize event dp-202216-6 and dp-023211-2 stack
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
CN115135984A (zh) 2019-12-23 2022-09-30 10X基因组学有限公司 可逆固定试剂及其使用方法
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
TW202142114A (zh) 2020-02-04 2021-11-16 美商陶氏農業科學公司 具有殺有害生物效用之組成物及與其相關之方法
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115335519A (zh) 2020-03-31 2022-11-11 凯杰有限公司 从固定的生物样品纯化核酸
WO2021207610A1 (en) 2020-04-10 2021-10-14 10X Genomics, Inc. Cold protease treatment method for preparing biological samples
WO2021212042A1 (en) 2020-04-16 2021-10-21 10X Genomics, Inc. Compositions and methods for use with fixed samples
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
CA3186978A1 (en) 2020-07-14 2022-01-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20230287475A1 (en) 2020-07-31 2023-09-14 10X Genomics, Inc. De-crosslinking compounds and methods of use for spatial analysis
US20230348928A1 (en) 2020-08-10 2023-11-02 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
EP4244379A1 (en) 2020-11-13 2023-09-20 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20230071935A1 (en) * 2021-08-25 2023-03-09 Matchstick Technologies, Inc. Microplate cycling thermo sonicator with programmable ultrasound, heating, and cooling for multi-analytical applications
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
TW202345696A (zh) 2022-05-18 2023-12-01 美商科迪華農業科技有限責任公司 具有殺有害生物效用之組成物及與其相關的方法
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830969A (en) 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
US4843155A (en) 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5620852A (en) 1990-11-14 1997-04-15 Hri Research, Inc. Nucleic acid preparation methods
US5284940A (en) 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5502166A (en) 1992-02-26 1996-03-26 Mitsubishi Chemical Corporation NMDH receptor proteins and genes encoding the same
AU6230594A (en) 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
US5876978A (en) * 1993-04-06 1999-03-02 Medical College Of Ohio Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5637687A (en) 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US5643767A (en) 1994-05-02 1997-07-01 The Rockefeller University Process for isolating cellular components
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
CA2153215A1 (en) 1994-07-06 1996-01-07 Lu Wang Treatment of paraffin embedded tissue for gene analysis
US5707802A (en) * 1995-01-13 1998-01-13 Ciba Corning Diagnostics Corp. Nucleic acid probes for the detection and identification of fungi
US5763169A (en) * 1995-01-13 1998-06-09 Chiron Diagnostics Corporation Nucleic acid probes for the detection and identification of fungi
US5777099A (en) 1995-02-24 1998-07-07 Biotecx Laboratories, Inc. RNA separation
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US5998151A (en) 1995-12-01 1999-12-07 The United States Of America As Represented By The Department Of Health And Human Services Methods for predicting the efficacy of a chemotherapeutic regimen for gastrointestinal cancers using antibodies specific for thymidylate synthase
US20020090642A1 (en) * 1995-12-11 2002-07-11 Patrick G. Johnston Methods for determining the prognosis of breast cancer using antibodies specific for thymidylate synthase
DE29601618U1 (de) 1996-01-31 1996-07-04 Invitek Gmbh Vorrichtung zur gleichzeitigen multiplen Isolierung
EP0791654A1 (en) 1996-02-21 1997-08-27 Jürgen A. Dr. Richt Polypeptides corresponding to the amino acid sequences of proteins p57 or p9.5 of Borna disease virus, nucleic acid fragments coding therefore and their use for diagnostic and immunization purposes
WO1997043407A1 (en) 1996-05-10 1997-11-20 Phylomed Corporation Methods for oxidizing disulfide bonds using ozone
JP2001522238A (ja) * 1997-03-31 2001-11-13 アボツト・ラボラトリーズ 胃腸管の疾患の検出に有用な試薬および方法
IL145422A0 (en) * 1999-03-18 2002-06-30 Exiqon As One step sample preparation and detection of nucleic acids in complex in biological samples
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens

Also Published As

Publication number Publication date
CN1891836B (zh) 2010-04-21
KR100723328B1 (ko) 2007-05-31
WO2001046402A1 (en) 2001-06-28
EP2363477A3 (en) 2013-07-31
US6610488B2 (en) 2003-08-26
NO330802B1 (no) 2011-07-18
US7960147B2 (en) 2011-06-14
AU784565B2 (en) 2006-05-04
EP1242594B1 (en) 2006-12-27
DE60032634T2 (de) 2007-10-04
US20020009794A1 (en) 2002-01-24
US6428963B2 (en) 2002-08-06
US6248535B1 (en) 2001-06-19
US20020009795A1 (en) 2002-01-24
US7919280B2 (en) 2011-04-05
NO20022945D0 (no) 2002-06-19
NO20022945L (no) 2002-08-06
US6613518B2 (en) 2003-09-02
US20010029018A1 (en) 2001-10-11
EP1743939B1 (en) 2011-09-07
IL150116A0 (en) 2002-12-01
US20090035761A1 (en) 2009-02-05
HK1055446A1 (en) 2004-01-09
CN1891836A (zh) 2007-01-10
BR0016569A (pt) 2003-07-15
DK1743939T3 (da) 2011-12-12
ATE523590T1 (de) 2011-09-15
EA006827B1 (ru) 2006-04-28
EP1242594A1 (en) 2002-09-25
DE60032634D1 (de) 2007-02-08
AU2006202986B2 (en) 2008-12-04
CN1420927A (zh) 2003-05-28
MXPA02006145A (es) 2004-08-23
ATE349518T1 (de) 2007-01-15
JP2003525033A (ja) 2003-08-26
CA2394618A1 (en) 2001-06-28
HK1098178A1 (en) 2007-07-13
EP1743939A2 (en) 2007-01-17
EA008832B1 (ru) 2007-08-31
US20040018551A1 (en) 2004-01-29
EP1743939A3 (en) 2007-05-09
IL150116A (en) 2012-01-31
EP2363477A2 (en) 2011-09-07
TWI277652B (en) 2007-04-01
US20060199197A1 (en) 2006-09-07
US7998707B2 (en) 2011-08-16
AU784565C (en) 2007-02-01
ES2373149T3 (es) 2012-01-31
AU1578501A (en) 2001-07-03
AU2006202986A1 (en) 2006-08-10
US20040091910A1 (en) 2004-05-13
AR027036A1 (es) 2003-03-12
EA200200698A1 (ru) 2003-12-25
CA2394618C (en) 2013-05-07
CN1269959C (zh) 2006-08-16
KR20030027878A (ko) 2003-04-07

Similar Documents

Publication Publication Date Title
EA200501772A1 (ru) Способы измерения экспрессии генов из фиксированного и залитого в парафин образца ткани
ATE266723T1 (de) Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen
ATE309536T1 (de) Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen
DE60238493D1 (de) Is von seltenen zellen verwendete markierte zellen
ATE469219T1 (de) Zusammensetzungen, methoden und reagentien zur nukleinsäureisolierung mittels oberflächenaktiven substanzen und proteasen
AU9058091A (en) Preparation of nucleic acid samples
ATE466932T1 (de) Vermehrung von viren in zellkultur
DK0979308T3 (da) Fremgangsåde til fremstilling af et diagnostisk standard gen-transkriptmönster
DE60144145D1 (de) Subtilisin-variante
HUP0302796A2 (hu) Eljárás humán szövetből nyert rákos sejtek szaporítására és berendezés a szövetminták feldolgozására
WO2002044418A3 (en) Expression analysis of fkbp nucleic acids and polypeptides useful in the diagnosis and treatment of prostate cancer
EP1090294A4 (en) METHOD FOR DETERMINING THE PRESENCE OF CANCER IN A SAMPLE BY DETECTING THE EXPRESSION OF A SSX GENE AND PEPTIDES DERIVED FROM SSX OR NY-ESO-1
ATE466938T1 (de) Prostata-spezifisches gen pcgem1 und methoden zu dessen verwendung zur erkennung, behandlung und prävention von prostatakrebs
DE69928395D1 (de) Cyclin-e2 proteine und dafür kodierende gene
DK0877758T3 (da) Oprenset SR-p70-protein
ATE255595T1 (de) Nephrin gen und protein
DE60113968D1 (de) Diagnose von brustkrebs unter verwendung von bcmp-7 als marker
SE9900615L (sv) Metod och kit för tidig förutsägelse av cancer
NZ518458A (en) Isolate DNA polymerase lambda and uses thereof
DE60038029D1 (de) Methoden und reagenzien zur in situ amplifizierung
MXPA02004825A (es) Genes especificamente expresados en celulas dendriticas humanas.
WO2001020024A3 (de) Verfahren zum ermitteln von nuklein- und/oder aminosäuresequenzen
WO2001022864A3 (en) Sequences characteristic of bladder cancer
DE60134956D1 (de) Gene differentiell experimiert in brudtkrebs
SE0201392D0 (sv) Fusion polypeptide, use thereof and methods employing it

Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): BY KZ MD

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM RU