AR027036A1 - Metodo para el aislamiento de rna de las muestras de tejido embebidas en parafina fijas con formalina - Google Patents

Metodo para el aislamiento de rna de las muestras de tejido embebidas en parafina fijas con formalina

Info

Publication number
AR027036A1
AR027036A1 ARP000106793A ARP000106793A AR027036A1 AR 027036 A1 AR027036 A1 AR 027036A1 AR P000106793 A ARP000106793 A AR P000106793A AR P000106793 A ARP000106793 A AR P000106793A AR 027036 A1 AR027036 A1 AR 027036A1
Authority
AR
Argentina
Prior art keywords
formfin
rna
insulation
fabric samples
fitted
Prior art date
Application number
ARP000106793A
Other languages
English (en)
Original Assignee
Univ Southern California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southern California filed Critical Univ Southern California
Publication of AR027036A1 publication Critical patent/AR027036A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
ARP000106793A 1999-12-20 2000-12-20 Metodo para el aislamiento de rna de las muestras de tejido embebidas en parafina fijas con formalina AR027036A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/469,338 US6248535B1 (en) 1999-12-20 1999-12-20 Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens

Publications (1)

Publication Number Publication Date
AR027036A1 true AR027036A1 (es) 2003-03-12

Family

ID=23863395

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP000106793A AR027036A1 (es) 1999-12-20 2000-12-20 Metodo para el aislamiento de rna de las muestras de tejido embebidas en parafina fijas con formalina

Country Status (20)

Country Link
US (8) US6248535B1 (es)
EP (3) EP2363477A3 (es)
JP (1) JP2003525033A (es)
KR (1) KR100723328B1 (es)
CN (2) CN1891836B (es)
AR (1) AR027036A1 (es)
AT (2) ATE349518T1 (es)
AU (2) AU784565C (es)
BR (1) BR0016569A (es)
CA (1) CA2394618C (es)
DE (1) DE60032634T2 (es)
DK (1) DK1743939T3 (es)
EA (2) EA008832B1 (es)
ES (1) ES2373149T3 (es)
HK (2) HK1055446A1 (es)
IL (2) IL150116A0 (es)
MX (1) MXPA02006145A (es)
NO (1) NO330802B1 (es)
TW (1) TWI277652B (es)
WO (1) WO2001046402A1 (es)

Families Citing this family (210)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1005633T3 (da) * 1997-08-20 2008-12-15 Univ Miami Vævsfikserings-dehydratiserings-fedtfjernelses-imprægneringsmetode med höj kvalitet og kontinuerligt gennemlöb
US6793890B2 (en) 1997-08-20 2004-09-21 The University Of Miami Rapid tissue processor
US6960439B2 (en) 1999-06-28 2005-11-01 Source Precision Medicine, Inc. Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US20060166227A1 (en) * 2000-06-20 2006-07-27 Stephen Kingsmore Protein expression profiling
US6602670B2 (en) * 2000-12-01 2003-08-05 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US6518416B1 (en) 2000-12-01 2003-02-11 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
EP1379686B1 (en) * 2000-12-01 2012-09-05 Response Genetics, Inc. Method of determining epidermal growth factor receptor and her2-neu gene expression and correlation of levels thereof with survival rates
US20020142328A1 (en) * 2000-12-01 2002-10-03 Danenberg Kathleen D. Method of determining a chemotherapeutic regimen by assaying gene expression in primary tumors
US7049059B2 (en) * 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
US6956111B2 (en) * 2001-03-02 2005-10-18 Response Genetics, Inc. Method of determining dihydropyrimidine dehydrogenase gene expression
US6686155B2 (en) * 2001-06-14 2004-02-03 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on glutathione-S-transferase pi expression
US20040091857A1 (en) * 2001-07-20 2004-05-13 Nallur Girish N. Gene expression profiling
JP4303592B2 (ja) * 2001-10-12 2009-07-29 ベクトン・ディキンソン・アンド・カンパニー 生体試料を輸送する方法及びバスケット装置
US7147826B2 (en) 2001-10-12 2006-12-12 Becton Dickinson And Company Method and apparatus for transporting biological samples
KR20040064275A (ko) 2001-11-09 2004-07-16 소스 프리시전 메디슨, 인코포레이티드 유전자 발현 프로파일을 이용한 질병의 동정, 모니터링,치료 및 생물학적 상태의 확인
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
DK2258872T3 (da) * 2002-03-13 2013-11-18 Genomic Health Inc Genekspressionsprofilering i biopsier af tumorvæv
AU2003257110A1 (en) 2002-07-31 2004-02-16 University Of Southern California Polymorphisms for predicting disease and treatment outcome
US7364846B2 (en) * 2002-10-11 2008-04-29 Molecular Devices Corporation Gene expression profiling from FFPE samples
CN100374576C (zh) * 2002-10-11 2008-03-12 阿克丘勒斯生物科学股份有限公司 来自ffpe样品的基因表达分布图
DK1572105T3 (da) * 2002-11-14 2011-10-17 Wayne John Cancer Inst Detektion af mikrometastase af melamoner og brystcancer i paraffinindlejret tumor drænende lymfeknuder ved hjælp af kvantitativ multimaker RT-PCR
US8039218B2 (en) * 2002-11-14 2011-10-18 John Wayne Cancer Institute Detection of cancer cells in body fluids
EP1570080A4 (en) 2002-11-15 2006-03-01 Genomic Health Inc GENE EXPRESSION PROFILING OF EGFR-POSITIVE CANCER DISEASE
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US20040231909A1 (en) 2003-01-15 2004-11-25 Tai-Yang Luh Motorized vehicle having forward and backward differential structure
EP1597391B1 (en) * 2003-02-20 2008-10-29 Genomic Health, Inc. Use of intronic rna to measure gene expression
CA2518569C (en) * 2003-03-10 2011-11-15 Expression Pathology, Inc. Liquid tissue preparation from histopathologically processed biological samples, tissues and cells
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
US20050164218A1 (en) * 2003-05-30 2005-07-28 David Agus Gene expression markers for response to EGFR inhibitor drugs
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
EP3170906B1 (en) 2003-06-24 2018-08-22 Genomic Health, Inc. Prediction of likelihood of cancer recurrence
WO2005008213A2 (en) * 2003-07-10 2005-01-27 Genomic Health, Inc. Expression profile algorithm and test for cancer prognosis
US8062897B2 (en) 2003-07-21 2011-11-22 Aureon Laboratories, Inc. Diagnostic histopathology using multiplex gene expression FISH
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
EP2295569A1 (en) 2003-07-25 2011-03-16 Ambion, Inc. Methods and compositions for preparing rna from a fixed sample
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
EP2311994A1 (en) * 2003-08-01 2011-04-20 Life Technologies Corporation Compositions and methods for preparing short RNA molecules and other nucleic acids
JP2007509613A (ja) * 2003-10-16 2007-04-19 ジェノミック ヘルス， インコーポレイテッド 遺伝子発現プロファイリングのためのｑＲＴ−ＰＣＲアッセイシステム
DK1676117T3 (da) * 2003-10-24 2009-11-09 Univ Miami Simplificeret forarbejdning af væv
US20050214316A1 (en) 2003-11-13 2005-09-29 Brown Thomas P Methods of characterizing infectious bursal disease virus
WO2005064019A2 (en) * 2003-12-23 2005-07-14 Genomic Health, Inc. Universal amplification of fragmented rna
CA2563074C (en) * 2004-04-09 2014-05-20 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
WO2005116256A2 (en) * 2004-04-16 2005-12-08 Wei-Sing Chu Device for extracting biological molecules from tissue specimens
US7794932B2 (en) 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
US20050239055A1 (en) * 2004-04-22 2005-10-27 Mariani Brian D Enterovirus primers and probes
US7332597B2 (en) * 2004-06-28 2008-02-19 University Of Kentucky Research Foundation Primers and probe to identify mycobacterium tuberculosis complex
WO2006012361A2 (en) 2004-07-01 2006-02-02 University Of Southern California Genetic markers for predicting disease and treatment outcome
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
EP3061833B1 (en) 2004-09-30 2018-03-14 Epigenomics AG Method for providing dna fragments derived from an archived sample
DE102004047772B4 (de) * 2004-09-30 2018-12-13 Infineon Technologies Ag Lateraler Halbleitertransistor
ATE550440T1 (de) * 2004-11-05 2012-04-15 Genomic Health Inc Molekulare indikatoren für brustkrebsprognose und vorhersage des ansprechens auf eine behandlung
PL1836629T3 (pl) * 2004-11-05 2020-06-15 Genomic Health, Inc. Przewidywanie odpowiedzi na chemioterapię z zastosowaniem markerów ekspresji genu
US20060141481A1 (en) * 2004-12-27 2006-06-29 Mariani Brian D HSV-1 and HSV-2 primers and probes
US7541144B2 (en) * 2005-01-31 2009-06-02 Agilent Technologies, Inc. RNA labeling method
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US7449297B2 (en) * 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
US20060252082A1 (en) * 2005-05-04 2006-11-09 University Of South Florida Predicting treatment response in cancer subjects
US20060269929A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods and kits for DNA purification on polymeric membranes at low ionic strength
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
US7544471B2 (en) * 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US20070054168A1 (en) * 2005-09-06 2007-03-08 Hao Chang Zinc/air cell
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP1777291A1 (en) * 2005-10-20 2007-04-25 Fundacion para la Investigacion Clinica y Molecular del Cancer de Pulmon Method for the isolation of mRNA from formalin fixed, paraffin-embedded tissue
US20070092883A1 (en) * 2005-10-26 2007-04-26 De Luwe Hoek Octrooien B.V. Methylation specific multiplex ligation-dependent probe amplification (MS-MLPA)
DE102005060738A1 (de) * 2005-12-16 2007-06-21 Qiagen Gmbh Verfahren zur Extraktion von Biomolekülen aus fixierten Geweben
CN100366632C (zh) * 2005-12-28 2008-02-06 南方医科大学 一种石蜡包埋组织提取蛋白质的方法
KR20090086415A (ko) * 2006-10-27 2009-08-12 조지 매이슨 인털렉춸 프로퍼티즈, 인코퍼레이티드 전이성 결장직장 암에 대한 검정
EP2147119B1 (en) * 2007-05-03 2011-05-25 Roche Diagnostics GmbH SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR
WO2008157316A1 (en) * 2007-06-14 2008-12-24 Creighton University Improved method for rna isolation
JP5479332B2 (ja) * 2007-06-15 2014-04-23 ユニヴァーシティ オブ サウス フロリダ 癌の診断方法および治療方法
WO2009002937A2 (en) 2007-06-22 2008-12-31 Response Genetics, Inc Methods for isolating long fragment rna from fixed samples
CA2704499C (en) 2007-11-07 2020-03-10 Genentech, Inc. Methods and compositions for assessing responsiveness of b-cell lymphoma to treatment with anti-cd40 antibodies
WO2009127350A1 (en) * 2008-04-15 2009-10-22 Universität Bern Method for optimized isolation of rna from fixed tissue
US20090298172A1 (en) * 2008-05-28 2009-12-03 Steven Paul Wheeler Histological specimen treatment apparatus and method
DE102008029356A1 (de) 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
WO2010012464A1 (en) * 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
EP2370803A2 (en) 2008-12-03 2011-10-05 The United States Government As Represented By The Department Of Veterans Affairs Pressure-assisted molecular recovery (pamr) of biomolecules, pressure-assisted antigen retrieval (paar), and pressure-assisted tissue histology (path)
EP2193831A1 (de) * 2008-12-05 2010-06-09 Qiagen GmbH Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe
DE102008061714A1 (de) 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
US8846878B1 (en) 2009-01-23 2014-09-30 Cubrc Corporation Method and device for isolating a protein sample
EP2210936A1 (en) * 2009-01-27 2010-07-28 Curetis AG Processing and analysis of viscous liquid biological samples
JP5836929B2 (ja) * 2009-04-18 2015-12-24 ジェネンテック， インコーポレイテッド 抗ｃｄ４０抗体を用いる治療に対するｂ細胞性リンパ腫の応答性を評価するための方法
US20120100997A1 (en) 2009-04-24 2012-04-26 University Of Southern California Cd133 polymorphisms and expression predict clinical outcome in patients with cancer
DE202009018981U1 (de) 2009-07-01 2015-03-11 Axagarius Gmbh & Co. Kg Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit dafür
DK2480660T5 (da) 2009-09-23 2020-11-09 Danisco Us Inc Hidtil ukendte glycosylhydrolaseenzymer og anvendelser heraf
US8329360B2 (en) 2009-12-04 2012-12-11 Taiwan Semiconductor Manufacturing Company, Ltd. Method and apparatus of providing overlay
MX2012007224A (es) 2009-12-23 2012-07-30 Danisco Us Inc Metodos para mejorar la eficacia de las reacciones de sacarificacion y fermentacion simultaneas.
JP5876834B2 (ja) * 2010-01-04 2016-03-02 キアジェン ゲイサーズバーグ インコーポレイテッド 固定された組織サンプルからの核酸またはタンパク質の回収のための方法、組成物、およびキット
ES2680545T3 (es) * 2010-02-26 2018-09-10 Qiagen Gmbh Proceso de aislamiento y/o purificación paralela de ARN y ADN
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
JP2013523143A (ja) 2010-04-08 2013-06-17 キアゲン ゲーエムベーハー 核酸を単離および精製するための方法
EP3399036B1 (en) 2010-04-08 2023-01-25 QIAGEN GmbH Chromatographic device and method for isolating and purifying nucleic acids
JP2013523178A (ja) 2010-04-12 2013-06-17 レスポンス ジェネティクス，インコーポレイティド Ｋｒａｓプライマーおよびプローブ
EP2388324A1 (de) * 2010-05-20 2011-11-23 Siemens Healthcare Diagnostics Inc. Kontrollmaterial für Nukleinsäureteste
EP2395082A1 (en) * 2010-06-14 2011-12-14 QIAGEN GmbH Extraction of nucleic acids from wax-embedded samples
CN103261873A (zh) * 2010-10-12 2013-08-21 生命科技股份有限公司 制备用于ffpe的质量对照材料的方法
KR101420094B1 (ko) * 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
TWI425977B (zh) 2010-11-24 2014-02-11 Ind Tech Res Inst 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組
EP2468862A1 (en) * 2010-12-23 2012-06-27 Qiagen GmbH Method and kit for processing wax-embedded biological samples
CN102146112B (zh) * 2011-01-25 2013-07-10 天根生化科技（北京）有限公司 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法
JP6013376B2 (ja) * 2011-02-28 2016-10-25 ダコ・デンマーク・エー／エス 包埋生体試料の前処置のための２相非混和系
CN102181431B (zh) * 2011-03-11 2012-12-26 大连医科大学 从石蜡包埋组织中提取总rna的方法
BR112013023757A2 (pt) 2011-03-17 2017-06-06 Danisco Us Inc método para redução de viscosidade no processo de sacarificação
JP6273198B2 (ja) 2011-03-28 2018-01-31 ジェロン・コーポレーションＧｅｒｏｎ Ｃｏｒｐｏｒａｔｉｏｎ 定量的ｐｃｒによる、ホルマリン固定パラフィン包埋（ｆｆｐｅ）試料におけるテロメア長測定
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
FR2982278A1 (fr) 2011-11-03 2013-05-10 Assist Publ Hopitaux De Paris Procede de classification d'echantillons de tissus fixes et inclus en paraffine
WO2013074927A1 (en) * 2011-11-16 2013-05-23 University Of Georgia Research Foundation, Inc. Method for isolating total rna from cells
IN2014CN04296A (es) 2011-11-17 2015-09-04 Rheonix Inc
CN103571824B (zh) 2012-08-09 2016-04-13 财团法人工业技术研究院 用于分离核酸的组合物及方法
EP3828270A1 (en) * 2012-09-28 2021-06-02 Cepheid Compositions for dna and rna extraction from fixed paraffin-embedded tissue samples
CN104884625A (zh) 2012-10-15 2015-09-02 先锋国际良种公司 增强cry内毒素的活性的方法和组合物
WO2014071182A1 (en) 2012-11-01 2014-05-08 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
EP2730653A1 (en) 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
US9200327B2 (en) 2012-11-30 2015-12-01 Geron Corporation Diagnostic markers for treating cell proliferative disorders with telomerase inhibitors
EP2970363B1 (en) 2013-03-14 2020-07-08 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US10023877B2 (en) 2013-03-15 2018-07-17 Pioneer Hi-Bred International, Inc. PHI-4 polypeptides and methods for their use
KR101748867B1 (ko) * 2013-04-19 2017-06-20 주식회사 젠큐릭스 조기 유방암 예후 예측 진단용 자동화 시스템
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
EP3032942B1 (en) 2013-08-16 2020-03-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3043635B1 (en) 2013-09-13 2020-02-12 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2015120276A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International Inc Insecticidal proteins and methods for their use
EP3102684B1 (en) 2014-02-07 2020-05-06 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2016000237A1 (en) 2014-07-03 2016-01-07 Pioneer Overseas Corporation Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes
WO2016044092A1 (en) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions and methods to control insect pests
US10435706B2 (en) 2014-10-16 2019-10-08 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
DE102014118532B4 (de) 2014-12-12 2017-03-23 Axagarius Gmbh & Co. Kg Vorrichtung mit einer Filterschicht und Verfahren zur Gewinnung von Nukleinsäuren aus Formalin-fixierten und in Paraffin eingebetteten Proben
WO2016109799A2 (en) * 2014-12-31 2016-07-07 The Rockefeller University Method of rna isolation from clinical samples
EA038923B1 (ru) 2015-03-11 2021-11-10 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидная днк-конструкция и способы её применения
ES2955916T3 (es) 2015-04-10 2023-12-11 Spatial Transcriptomics Ab Análisis múltiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
US10365189B2 (en) 2015-05-07 2019-07-30 Steven Wheeler Histological specimen treatment
RU2017144238A (ru) 2015-05-19 2019-06-19 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
EA033170B1 (ru) * 2015-05-21 2019-09-30 Государственное Учреждение Образования "Белорусская Медицинская Академия Последипломного Образования" (Гуо "Белмапо") Способ определения экспрессии гена gstm1 в образцах из парафин-фиксированных срезов опухолевой ткани молочной железы
AU2016278142A1 (en) 2015-06-16 2017-11-30 E. I. Du Pont De Nemours And Company Compositions and methods to control insect pests
MX2018000615A (es) 2015-07-13 2018-08-01 Pivot Bio Inc Metodos y composiciones para mejorar atributos de plantas.
US10465182B2 (en) 2015-07-24 2019-11-05 Cepheid Compositions and methods for DNA and RNA extraction from tissue samples
MX2018001523A (es) 2015-08-06 2018-03-15 Pioneer Hi Bred Int Proteinas insecticidas derivadas de plantas y metodos para su uso.
EP3341483B1 (en) 2015-08-28 2019-12-18 Pioneer Hi-Bred International, Inc. Ochrobactrum-mediated transformation of plants
WO2017062412A1 (en) 2015-10-05 2017-04-13 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
KR101749419B1 (ko) 2015-10-15 2017-07-03 주식회사 어큐진 파라핀포매된 샘플로부터 dna를 추출하는 방법
KR20180081561A (ko) 2015-11-06 2018-07-16 벤타나 메디컬 시스템즈, 인코포레이티드 대표 진단법
US20180325119A1 (en) 2015-12-18 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN117003840A (zh) 2016-04-14 2023-11-07 先锋国际良种公司 具有改善的活性谱的杀昆虫多肽及其用途
US11028407B2 (en) 2016-04-19 2021-06-08 Pioneer Hi-Bred International, Inc. Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof
EP3960863A1 (en) 2016-05-04 2022-03-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20190185867A1 (en) 2016-06-16 2019-06-20 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US20190194676A1 (en) 2016-06-24 2019-06-27 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
EP3954202A1 (en) 2016-07-01 2022-02-16 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US20210292778A1 (en) 2016-07-12 2021-09-23 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
EP4050021A1 (en) 2016-11-01 2022-08-31 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN110088123B (zh) 2016-12-14 2023-10-20 先锋国际良种公司 杀昆虫蛋白及其使用方法
CA3046226A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
JP6877550B2 (ja) * 2016-12-22 2021-05-26 ヴェンタナ メディカル システムズ， インク． 組織試料用の全自動核酸抽出方法
CN110799474B (zh) 2017-01-12 2022-07-26 皮沃特生物公司 用于改良植物性状的方法及组合物
WO2018140214A1 (en) 2017-01-24 2018-08-02 Pioneer Hi-Bred International, Inc. Nematicidal protein from pseudomonas
WO2018148001A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International Inc Insecticidal combinations of plant derived insecticidal proteins and methods for their use
BR112019024827A2 (pt) 2017-05-26 2020-06-16 Pioneer Hi-Bred International, Inc. Construto de dna, planta transgênica ou progênie da mesma, composição e método para controlar uma população de pragas de insetos
CN107271239A (zh) * 2017-07-06 2017-10-20 浙江大学 一种溶解石蜡组织切片的方法及应用
US20200165626A1 (en) 2017-10-13 2020-05-28 Pioneer Hi-Bred International, Inc. Virus-induced gene silencing technology for insect control in maize
US11993778B2 (en) 2017-10-25 2024-05-28 Pivot Bio, Inc. Methods and compositions for improving engineered microbes that fix nitrogen
EP3755797A1 (en) 2018-02-22 2020-12-30 Zymergen, Inc. Method for creating a genomic library enriched for bacillus and identification of novel cry toxins
WO2019169227A1 (en) 2018-03-02 2019-09-06 Zymergen Inc. Insecticidal protein discovery platform and insecticidal proteins discovered therefrom
US20210002657A1 (en) 2018-03-02 2021-01-07 Pioneer Hi-Bred International, Inc. Plant health assay
CA3096516A1 (en) 2018-05-22 2019-11-28 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
US11963530B2 (en) 2018-06-27 2024-04-23 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
EP3844283A1 (en) 2018-08-29 2021-07-07 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3894592A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating spatial arrays with gradients
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN110376036A (zh) * 2019-07-30 2019-10-25 河南赛诺特生物技术有限公司 一种肿瘤细胞系免疫组化对照品的制备方法及肿瘤细胞系免疫组化对照品
CN110361242B (zh) * 2019-08-14 2022-01-18 武汉赛维尔生物科技有限公司 一种用于眼球组织的固定液以及眼球组织制片的预处理方法
WO2021076346A1 (en) 2019-10-18 2021-04-22 Pioneer Hi-Bred International, Inc. Maize event dp-202216-6 and dp-023211-2 stack
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
CN115135984A (zh) 2019-12-23 2022-09-30 10X基因组学有限公司 可逆固定试剂及其使用方法
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
CN115038794A (zh) 2019-12-23 2022-09-09 10X基因组学有限公司 在基于分区的测定中使用固定生物样品的组合物和方法
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
TW202142114A (zh) 2020-02-04 2021-11-16 美商陶氏農業科學公司 具有殺有害生物效用之組成物及與其相關之方法
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
JP2023521579A (ja) 2020-03-31 2023-05-25 キアゲン ゲーエムベーハー 固定された生体試料からの核酸精製
WO2021207610A1 (en) 2020-04-10 2021-10-14 10X Genomics, Inc. Cold protease treatment method for preparing biological samples
EP4136227A1 (en) 2020-04-16 2023-02-22 10X Genomics, Inc. Compositions and methods for use with fixed samples
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US20230235352A1 (en) 2020-07-14 2023-07-27 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP4189110A1 (en) 2020-07-31 2023-06-07 10X Genomics, Inc. De-crosslinking compounds and methods of use for spatial analysis
EP4192848A1 (en) 2020-08-10 2023-06-14 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
EP4244379A1 (en) 2020-11-13 2023-09-20 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
CN114854824A (zh) * 2021-02-03 2022-08-05 深圳华大基因股份有限公司 建库方法及应用
US20230071935A1 (en) * 2021-08-25 2023-03-09 Matchstick Technologies, Inc. Microplate cycling thermo sonicator with programmable ultrasound, heating, and cooling for multi-analytical applications
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
TW202345696A (zh) 2022-05-18 2023-12-01 美商科迪華農業科技有限責任公司 具有殺有害生物效用之組成物及與其相關的方法
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830969A (en) 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
US4843155A (en) 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5620852A (en) 1990-11-14 1997-04-15 Hri Research, Inc. Nucleic acid preparation methods
US5284940A (en) 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5502166A (en) 1992-02-26 1996-03-26 Mitsubishi Chemical Corporation NMDH receptor proteins and genes encoding the same
JP3615545B2 (ja) 1993-02-01 2005-02-02 キアゲン・エヌ・ブイ 第四級アンモニウム塩界面活性剤及びそのｒｎａの単離剤
US5876978A (en) * 1993-04-06 1999-03-02 Medical College Of Ohio Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5637687A (en) 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US5643767A (en) 1994-05-02 1997-07-01 The Rockefeller University Process for isolating cellular components
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
CA2153215A1 (en) 1994-07-06 1996-01-07 Lu Wang Treatment of paraffin embedded tissue for gene analysis
US5763169A (en) * 1995-01-13 1998-06-09 Chiron Diagnostics Corporation Nucleic acid probes for the detection and identification of fungi
US5707802A (en) * 1995-01-13 1998-01-13 Ciba Corning Diagnostics Corp. Nucleic acid probes for the detection and identification of fungi
US5777099A (en) 1995-02-24 1998-07-07 Biotecx Laboratories, Inc. RNA separation
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US5998151A (en) 1995-12-01 1999-12-07 The United States Of America As Represented By The Department Of Health And Human Services Methods for predicting the efficacy of a chemotherapeutic regimen for gastrointestinal cancers using antibodies specific for thymidylate synthase
US20020090642A1 (en) * 1995-12-11 2002-07-11 Patrick G. Johnston Methods for determining the prognosis of breast cancer using antibodies specific for thymidylate synthase
DE29601618U1 (de) 1996-01-31 1996-07-04 InViTek GmbH, 13125 Berlin Vorrichtung zur gleichzeitigen multiplen Isolierung
EP0791654A1 (en) 1996-02-21 1997-08-27 Jürgen A. Dr. Richt Polypeptides corresponding to the amino acid sequences of proteins p57 or p9.5 of Borna disease virus, nucleic acid fragments coding therefore and their use for diagnostic and immunization purposes
EP0912726A4 (en) * 1996-05-10 2001-07-18 Phylomed Corp METHODS FOR OXIDIZING BISULFIDE LINKS WITH OZONE
WO1998044159A1 (en) * 1997-03-31 1998-10-08 Abbott Laboratories Reagents and methods useful for detecting diseases of the gastrointestinal tract
ATE314488T1 (de) * 1999-03-18 2006-01-15 Exiqon As Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens

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