BR0016569A - Processo de isolamento de rna de espécimes de tecido embutidos - parafina fixados - formalina - Google Patents

Processo de isolamento de rna de espécimes de tecido embutidos - parafina fixados - formalina

Info

Publication number
BR0016569A
BR0016569A BR0016569-7A BR0016569A BR0016569A BR 0016569 A BR0016569 A BR 0016569A BR 0016569 A BR0016569 A BR 0016569A BR 0016569 A BR0016569 A BR 0016569A
Authority
BR
Brazil
Prior art keywords
formalin
rna isolation
fixed paraffin
tissue
tissue specimens
Prior art date
Application number
BR0016569-7A
Other languages
English (en)
Inventor
Kathleen Danenberg
Peter Danenberg
Steven Swenson
Original Assignee
Univ Southern California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southern California filed Critical Univ Southern California
Publication of BR0016569A publication Critical patent/BR0016569A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

"PROCESSO DE ISOLAMENTO DE RNA DE ESPéCIMES DE TECIDO EMBUTIDOS - PARAFINA FIXADOS - FORMALINA". São mostrados processos para isolamento rápido, simples e confiável de RNA de amostras de tecido embutidas em parafina fixadas com formalina. RNA purificado desta maneira pode ser usado para monitorar níveis de expressão de gene. A amostra de tecido pode ser um tecido de tumor ou outro tecido patológico.
BR0016569-7A 1999-12-20 2000-10-31 Processo de isolamento de rna de espécimes de tecido embutidos - parafina fixados - formalina BR0016569A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/469,338 US6248535B1 (en) 1999-12-20 1999-12-20 Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
PCT/US2000/030012 WO2001046402A1 (en) 1999-12-20 2000-10-31 Method for isolation of rna from formalin-fixed paraffin-embedded tissue specimens

Publications (1)

Publication Number Publication Date
BR0016569A true BR0016569A (pt) 2003-07-15

Family

ID=23863395

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0016569-7A BR0016569A (pt) 1999-12-20 2000-10-31 Processo de isolamento de rna de espécimes de tecido embutidos - parafina fixados - formalina

Country Status (20)

Country Link
US (8) US6248535B1 (pt)
EP (3) EP1242594B1 (pt)
JP (1) JP2003525033A (pt)
KR (1) KR100723328B1 (pt)
CN (2) CN1269959C (pt)
AR (1) AR027036A1 (pt)
AT (2) ATE523590T1 (pt)
AU (2) AU784565C (pt)
BR (1) BR0016569A (pt)
CA (1) CA2394618C (pt)
DE (1) DE60032634T2 (pt)
DK (1) DK1743939T3 (pt)
EA (2) EA006827B1 (pt)
ES (1) ES2373149T3 (pt)
HK (2) HK1055446A1 (pt)
IL (2) IL150116A0 (pt)
MX (1) MXPA02006145A (pt)
NO (1) NO330802B1 (pt)
TW (1) TWI277652B (pt)
WO (1) WO2001046402A1 (pt)

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