EA200200698A1 - Способ выделения рнк из фиксированных формалином и залитых в парафин образцов ткани - Google Patents

Способ выделения рнк из фиксированных формалином и залитых в парафин образцов ткани

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Publication number
EA200200698A1
EA200200698A1 EA200200698A EA200200698A EA200200698A1 EA 200200698 A1 EA200200698 A1 EA 200200698A1 EA 200200698 A EA200200698 A EA 200200698A EA 200200698 A EA200200698 A EA 200200698A EA 200200698 A1 EA200200698 A1 EA 200200698A1
Authority
EA
Eurasian Patent Office
Prior art keywords
tissue samples
filled
rna isolation
paraffin tissue
fixed formalin
Prior art date
Application number
EA200200698A
Other languages
English (en)
Other versions
EA006827B1 (ru
Inventor
Кэтлин Даненберг
Питер Даненберг
Стивен Свенсон
Original Assignee
Юниверсити Оф Саутерн Калифорния
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Юниверсити Оф Саутерн Калифорния filed Critical Юниверсити Оф Саутерн Калифорния
Publication of EA200200698A1 publication Critical patent/EA200200698A1/ru
Publication of EA006827B1 publication Critical patent/EA006827B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Abstract

Описаны быстрые, надежные и простые способы выделения РНК из фиксированных формалинов и залитых в парафин образцов тканей. РНК, очищенная таким способом, может быть использована для мониторинга уровней экспрессии генов. Образцом ткани может быть опухоль или другая патологическая ткань.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200200698A 1999-12-20 2000-10-31 Способ выделения рнк из залитых в парафин образцов ткани EA006827B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/469,338 US6248535B1 (en) 1999-12-20 1999-12-20 Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
PCT/US2000/030012 WO2001046402A1 (en) 1999-12-20 2000-10-31 Method for isolation of rna from formalin-fixed paraffin-embedded tissue specimens

Publications (2)

Publication Number Publication Date
EA200200698A1 true EA200200698A1 (ru) 2003-12-25
EA006827B1 EA006827B1 (ru) 2006-04-28

Family

ID=23863395

Family Applications (2)

Application Number Title Priority Date Filing Date
EA200501772A EA008832B1 (ru) 1999-12-20 2000-10-31 Способы измерения экспрессии генов из фиксированного и залитого в парафин образца ткани
EA200200698A EA006827B1 (ru) 1999-12-20 2000-10-31 Способ выделения рнк из залитых в парафин образцов ткани

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EA200501772A EA008832B1 (ru) 1999-12-20 2000-10-31 Способы измерения экспрессии генов из фиксированного и залитого в парафин образца ткани

Country Status (20)

Country Link
US (8) US6248535B1 (ru)
EP (3) EP2363477A3 (ru)
JP (1) JP2003525033A (ru)
KR (1) KR100723328B1 (ru)
CN (2) CN1269959C (ru)
AR (1) AR027036A1 (ru)
AT (2) ATE349518T1 (ru)
AU (2) AU784565C (ru)
BR (1) BR0016569A (ru)
CA (1) CA2394618C (ru)
DE (1) DE60032634T2 (ru)
DK (1) DK1743939T3 (ru)
EA (2) EA008832B1 (ru)
ES (1) ES2373149T3 (ru)
HK (2) HK1055446A1 (ru)
IL (2) IL150116A0 (ru)
MX (1) MXPA02006145A (ru)
NO (1) NO330802B1 (ru)
TW (1) TWI277652B (ru)
WO (1) WO2001046402A1 (ru)

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