DK3325647T3 - Sammensætninger og fremgangsmåder til dna- og rna-ekstraktion fra vævsprøver - Google Patents

Sammensætninger og fremgangsmåder til dna- og rna-ekstraktion fra vævsprøver Download PDF

Info

Publication number
DK3325647T3
DK3325647T3 DK16742522.2T DK16742522T DK3325647T3 DK 3325647 T3 DK3325647 T3 DK 3325647T3 DK 16742522 T DK16742522 T DK 16742522T DK 3325647 T3 DK3325647 T3 DK 3325647T3
Authority
DK
Denmark
Prior art keywords
dna
procedures
compositions
rna extraction
table samples
Prior art date
Application number
DK16742522.2T
Other languages
English (en)
Inventor
Kenneth E Ho
Original Assignee
Cepheid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cepheid filed Critical Cepheid
Application granted granted Critical
Publication of DK3325647T3 publication Critical patent/DK3325647T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK16742522.2T 2015-07-24 2016-07-12 Sammensætninger og fremgangsmåder til dna- og rna-ekstraktion fra vævsprøver DK3325647T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562196774P 2015-07-24 2015-07-24
PCT/US2016/041917 WO2017019293A1 (en) 2015-07-24 2016-07-12 Compositions and methods for dna and rna extraction from tissue samples

Publications (1)

Publication Number Publication Date
DK3325647T3 true DK3325647T3 (da) 2020-05-18

Family

ID=56550375

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16742522.2T DK3325647T3 (da) 2015-07-24 2016-07-12 Sammensætninger og fremgangsmåder til dna- og rna-ekstraktion fra vævsprøver

Country Status (9)

Country Link
US (1) US10465182B2 (da)
EP (2) EP3705582A1 (da)
JP (1) JP7099951B2 (da)
CN (1) CN108124451B (da)
AU (1) AU2016298048B2 (da)
CA (1) CA2993447C (da)
DK (1) DK3325647T3 (da)
ES (1) ES2795048T3 (da)
WO (1) WO2017019293A1 (da)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2900819B1 (en) 2012-09-28 2020-10-28 Cepheid Methods for dna and rna extraction from fixed paraffin-embedded tissue samples
JP7099951B2 (ja) 2015-07-24 2022-07-12 セファイド 組織試料からのdna及びrna抽出のための組成物及び方法
WO2019246584A1 (en) * 2018-06-21 2019-12-26 Genomic Health, Inc. Systems and methods for pre-analytical substrate processing
CN109234269B (zh) * 2018-09-21 2021-10-12 深圳金普迈生物科技有限公司 从甲醛固定的微量样品中提取并扩增dna片段的方法
KR102041333B1 (ko) * 2018-12-24 2019-11-06 주식회사 덴오믹스 세포 용해 조성물 및 이를 이용한 핵산의 추출 방법
CN110835628B (zh) * 2019-11-25 2021-07-27 宁波艾捷康宁生物科技有限公司 一种石蜡切片基因组dna提取的除蜡裂解液及提取试剂盒和提取方法
CN111057704B (zh) * 2019-12-31 2022-07-05 广州市金圻睿生物科技有限责任公司 一种石蜡切片样本dna提取的脱蜡裂解结合液、试剂盒及方法
EP4308729A1 (en) 2021-03-16 2024-01-24 Cepheid Methods for discriminating bacterial and viral infections in a human subject
CN117106887A (zh) 2021-10-22 2023-11-24 塞弗德公司 诊断和治疗结核病的组合物和方法
TWI771253B (zh) * 2021-12-27 2022-07-11 財團法人工業技術研究院 戶外用的簡易式核酸萃取套組以及於戶外萃取樣本的核酸的方法

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958349A (en) 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
WO1999033559A1 (en) 1997-12-24 1999-07-08 Cepheid Integrated fluid manipulation cartridge
CA2270023C (en) 1998-04-28 2010-10-12 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
US6469159B1 (en) 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
US6818185B1 (en) 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
EP1180135B1 (en) 1999-05-28 2005-08-17 Cepheid Apparatus and method for cell disruption
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US6423488B1 (en) * 2000-01-15 2002-07-23 Avigenics, Inc High throughput screening assay for detecting a DNA sequence
US6403037B1 (en) 2000-02-04 2002-06-11 Cepheid Reaction vessel and temperature control system
CN101268189A (zh) * 2003-04-02 2008-09-17 亚钧阔特生命科学公司 分离核酸的方法
US20050042656A1 (en) 2003-07-09 2005-02-24 Davis James C. Room temperature elution of nucleic acids
ATE476505T1 (de) 2005-02-11 2010-08-15 Invitrogen Dynal As Verfahren zur isolierung von nukleinsäuren mit verwendung von ethylenglykol-multimeren
CN101243186A (zh) * 2005-09-28 2008-08-13 富士胶片株式会社 核酸的提取方法
JP2007117084A (ja) * 2005-09-28 2007-05-17 Fujifilm Corp 核酸抽出法
EP1783645A1 (en) 2005-11-08 2007-05-09 Actigenics Methods for the identification of microRNA and their applications in research and human health
DE102005057334A1 (de) * 2005-11-28 2007-06-06 Aj Innuscreen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien
JP2007325562A (ja) * 2006-06-09 2007-12-20 Fujifilm Corp 核酸抽出法
JP2008043332A (ja) 2006-08-17 2008-02-28 Panomics Inc 組織スライドからの核酸定量
US8080645B2 (en) 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
WO2008035991A2 (en) 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method
EP2262893B1 (en) 2008-04-15 2013-04-24 Universität Bern Method for optimized isolation of rna from fixed tissue
EP2193831A1 (de) 2008-12-05 2010-06-09 Qiagen GmbH Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe
US20100240049A1 (en) 2009-01-16 2010-09-23 Cepheid Methods of Detecting Cervical Cancer
EP2401406A4 (en) 2009-02-25 2012-09-19 Cepheid METHODS OF DETECTING LUNG CANCER
WO2011032124A1 (en) 2009-09-14 2011-03-17 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
RS56636B1 (sr) * 2010-01-07 2018-03-30 Bigtec Private Ltd Postupak i komplet za izolaciju nukleinskih kiselina
WO2011104032A1 (en) 2010-02-26 2011-09-01 Qiagen Gmbh Method for isolating rna from a rna and dna containing sample
US20130023655A1 (en) * 2010-04-08 2013-01-24 Qiagen Gmbh Method for precipitating anionic surfactant ions in the presence of nucleic acids
EP2395082A1 (en) 2010-06-14 2011-12-14 QIAGEN GmbH Extraction of nucleic acids from wax-embedded samples
JP2014526255A (ja) 2011-09-13 2014-10-06 キアゲン ゲーエムベーハー 獣医学的全血試料から核酸を単離するための方法
GB201209229D0 (en) 2012-05-25 2012-07-04 Epistem Ltd Nucleic acid extraction
US9145553B2 (en) 2012-06-15 2015-09-29 Ut-Battelle, Llc Method for isolating nucleic acids
EP2708890B1 (en) 2012-09-14 2015-11-18 Molecular Devices (New Milton) Ltd Method of selecting a monoclonal cell colony
EP2900819B1 (en) 2012-09-28 2020-10-28 Cepheid Methods for dna and rna extraction from fixed paraffin-embedded tissue samples
WO2014146782A1 (en) 2013-03-18 2014-09-25 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
EP2793026A1 (en) 2013-04-18 2014-10-22 Centre National de la Recherche Scientifique (CNRS) New method for monitoring cancer and/or inflammatory reaction based on relb phosphorylation
CN104152438B (zh) * 2014-08-15 2017-05-17 四川农业大学 用于提取家禽dna的细胞裂解液、试剂盒和方法
AU2016277943B2 (en) * 2015-06-15 2022-09-01 Cepheid Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or mRNA expression levels in an automated reaction cartridge
JP7099951B2 (ja) 2015-07-24 2022-07-12 セファイド 組織試料からのdna及びrna抽出のための組成物及び方法

Also Published As

Publication number Publication date
JP7099951B2 (ja) 2022-07-12
ES2795048T3 (es) 2020-11-20
EP3325647B1 (en) 2020-02-19
US10465182B2 (en) 2019-11-05
JP2018524016A (ja) 2018-08-30
AU2016298048A1 (en) 2018-02-15
CN108124451B (zh) 2022-09-09
EP3705582A1 (en) 2020-09-09
CA2993447A1 (en) 2017-02-02
CN108124451A (zh) 2018-06-05
CA2993447C (en) 2024-01-02
US20170022493A1 (en) 2017-01-26
AU2016298048B2 (en) 2022-08-04
EP3325647A1 (en) 2018-05-30
WO2017019293A1 (en) 2017-02-02

Similar Documents

Publication Publication Date Title
DK3325647T3 (da) Sammensætninger og fremgangsmåder til dna- og rna-ekstraktion fra vævsprøver
DK3325669T3 (da) Sammensætninger og fremgangsmåder til RNA-analyse
DK3390631T3 (da) Fremgangsmåder og sammensætninger til t-rna-baseret guide-rna-ekspression
SG10202108763UA (en) Methods and compositions for nucleic acid analysis
ZA201801578B (en) Methods and compositions for marker-free genome modification
DK3108009T3 (da) Fremgangsmåder og sammensætninger til DNA-profilering
DK3247371T3 (da) Fremgangsmåder til oprensning af cannabinoider, sammensætninger og kits dertil
DK3782639T3 (da) Sammensætninger og fremgangsmåder til internalisering af enzymer
DK3307872T3 (da) Fremgangsmåder og sammensætninger til effektiv indgivelse af nukleinsyrer og rna-baserede antimikrober
DK3092256T3 (da) Forbindelser og sammensætninger til immunterapi
DK3218489T3 (da) Hepatitis-B-virus (HBV)-IRNA-sammensætninger og fremgangsmåder til anvendelse deraf
DK3386550T3 (da) Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer
DK3080274T3 (da) Fremgangsmåder og sammensætninger til genom-manipulation
DK3114225T3 (da) Sammensætning og fremgangsmåde til stabilisering af nukleinsyrer i biologiske prøver
HK1219948A1 (zh) 用於保存生物樣品的穩定劑
DK3234160T3 (da) Sammensætninger og fremgangsmåder til hjælper-stamme-medieret svampegenommodifikation
DK3699289T3 (da) Prøveklargøring til nukleinsyreamplifikation
DK3172233T3 (da) Anti-cd74-antistoffer, sammensætninger omfattende anti-cd74- antistoffer og fremgangsmåder til anvendelse af anti-cd74-antistoffer
DK3204386T3 (da) Substituerede aminopurinforbindelser, sammensætninger deraf og fremgangsmåder til behandling dermed
DK3353168T3 (da) Forbindelser og fremgangsmåder til at inhibere jak
DK3384274T3 (da) Fremgangsmåder til prøvekarakterisering
DK3408407T3 (da) Fremgangsmåder til påvisning af cpg-methylering af tumorafledt dna i blodprøver
DK3129779T3 (da) Fremgangsmåder og sammensætninger til påvisning af fejlfoldede proteiner
DK3169696T3 (da) Fremgangsmåde til ekstraktion af opløselige proteiner fra mikroalgebiomasse
DK3359281T3 (da) Fremgangsmåde til udtrækning af CO2