ATE314488T1 - Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben - Google Patents

Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben

Info

Publication number
ATE314488T1
ATE314488T1 AT00910584T AT00910584T ATE314488T1 AT E314488 T1 ATE314488 T1 AT E314488T1 AT 00910584 T AT00910584 T AT 00910584T AT 00910584 T AT00910584 T AT 00910584T AT E314488 T1 ATE314488 T1 AT E314488T1
Authority
AT
Austria
Prior art keywords
nucleic acid
nucleic acids
sample preparation
biological samples
methods
Prior art date
Application number
AT00910584T
Other languages
English (en)
Inventor
Jan Skouv
Original Assignee
Exiqon As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exiqon As filed Critical Exiqon As
Application granted granted Critical
Publication of ATE314488T1 publication Critical patent/ATE314488T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C07K14/003Peptide-nucleic acids (PNAs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT00910584T 1999-03-18 2000-03-17 Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben ATE314488T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199900384 1999-03-18
PCT/DK2000/000128 WO2000056920A1 (en) 1999-03-18 2000-03-17 One step sample preparation and detection of nucleic acids in complex biological samples

Publications (1)

Publication Number Publication Date
ATE314488T1 true ATE314488T1 (de) 2006-01-15

Family

ID=8092891

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00910584T ATE314488T1 (de) 1999-03-18 2000-03-17 Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben

Country Status (11)

Country Link
US (2) US6303315B1 (de)
EP (1) EP1161561B1 (de)
JP (1) JP4975905B2 (de)
KR (1) KR20020007332A (de)
CN (1) CN1350591A (de)
AT (1) ATE314488T1 (de)
AU (1) AU3274600A (de)
CA (1) CA2366231C (de)
DE (1) DE60025172T2 (de)
IL (1) IL145422A0 (de)
WO (1) WO2000056920A1 (de)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US6436640B1 (en) * 1999-03-18 2002-08-20 Exiqon A/S Use of LNA in mass spectrometry
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US20020072060A1 (en) * 2000-07-19 2002-06-13 Getts Robert C. Methods for detecting and assaying nucleic acid sequences
AU2001247414A1 (en) 2000-03-14 2001-09-24 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
US6962906B2 (en) 2000-03-14 2005-11-08 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
US20020022231A1 (en) * 2000-08-18 2002-02-21 Vaughn Vasil Method for identifying modulators of DNA structure-specific binding proteins by high-throughput screening
EP1354064A2 (de) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
US7049059B2 (en) * 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
US20030077609A1 (en) * 2001-03-25 2003-04-24 Jakobsen Mogens Havsteen Modified oligonucleotides and uses thereof
GB0114852D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Happiar mapping
US7888324B2 (en) 2001-08-01 2011-02-15 Genzyme Corporation Antisense modulation of apolipoprotein B expression
US7407943B2 (en) 2001-08-01 2008-08-05 Isis Pharmaceuticals, Inc. Antisense modulation of apolipoprotein B expression
AU2002332778A1 (en) * 2001-08-31 2003-03-18 Datascope Investment Corp. Methods for blocking nonspecific hybridizations of nucleic acid sequences
DE10152925A1 (de) * 2001-10-26 2003-05-08 Febit Ag Asymmetrische Sonden
JP2003199568A (ja) * 2002-01-10 2003-07-15 Nichirei Corp Dna増幅反応の効率向上方法
DE10208794A1 (de) * 2002-02-28 2003-09-04 Degussa Bioactives Gmbh Verfahren zum Sortieren einsträngiger Nucleinsäuren
ATE432347T1 (de) * 2002-06-24 2009-06-15 Exiqon As Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen
US20040018634A1 (en) * 2002-07-29 2004-01-29 Kiamars Hajizadeh Sample-collection and preparation device and method
US20040018120A1 (en) * 2002-07-29 2004-01-29 Craig Rappin Sample preparation device and method
US20040018575A1 (en) * 2002-07-29 2004-01-29 Craig Rappin Sample preparation device and method
US20060147924A1 (en) * 2002-09-11 2006-07-06 Ramsing Neils B Population of nucleic acids including a subpopulation of lna oligomers
US20040219565A1 (en) * 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
US7511131B2 (en) 2002-11-13 2009-03-31 Genzyme Corporation Antisense modulation of apolipoprotein B expression
KR101061891B1 (ko) 2003-02-05 2011-09-02 가부시키가이샤 한도오따이 에네루기 켄큐쇼 배선의 제작 방법
CN1580283A (zh) * 2003-08-13 2005-02-16 清华大学 一种检测核酸分子的方法
US7897378B2 (en) 2004-03-18 2011-03-01 Roche Molecular Systems, Inc. Method and device for purifying nucleic acids
US20050287558A1 (en) 2004-05-05 2005-12-29 Crooke Rosanne M SNPs of apolipoprotein B and modulation of their expression
US20060014183A1 (en) * 2004-06-10 2006-01-19 Pfundheller Henrik M Extendable probes
EP1809765A2 (de) * 2004-11-04 2007-07-25 Roche Diagnostics GmbH Klassifizierung von aml (akuter myeloider leukämie)
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
US20060234277A1 (en) * 2005-03-31 2006-10-19 Amgen Inc. Method for selectively blocking hemoglobin RNA amplification
US8067208B2 (en) 2005-06-30 2011-11-29 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using multidimensional probe analysis
CA2622712A1 (en) * 2005-09-16 2007-03-29 Primera Biosystems, Inc. Compositions and methods for purifying nucleic acids
CA3044969A1 (en) 2006-05-05 2007-12-21 Ionis Pharmaceuticals, Inc. Compounds and methods for modulating gene expression
DE102006031764B4 (de) * 2006-07-06 2009-10-01 Aj Innuscreen Gmbh Verfahren zur parallelen Isolierung doppel- und einzelsträngiger Nukleinsäuren sowie zur selektiven Entfernung doppelsträngiger Nukleinsäuren aus einem Gemisch von doppel- und einzelsträngigen Nukleinsäuren
CA2659632A1 (en) * 2006-08-01 2008-02-07 Applera Corporation Detection of analytes and nucleic acids
US8846313B2 (en) * 2006-11-06 2014-09-30 Clondiag Gmbh Assays
US8293684B2 (en) * 2006-11-29 2012-10-23 Exiqon Locked nucleic acid reagents for labelling nucleic acids
GB0625595D0 (en) * 2006-12-21 2007-01-31 Oxford Gene Tech Ip Ltd Sample analyser
RU2559536C2 (ru) 2007-03-24 2015-08-10 Джензим Корпорейшн Введение антисмысловых олигонуклеотидов, комплементарных человеческому аполипопротеину в
WO2009018034A1 (en) * 2007-07-27 2009-02-05 Ge Healthcare Bio-Sciences Corp. An improved nucleic acid purification method
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
WO2010107838A1 (en) 2009-03-16 2010-09-23 Isis Pharmaceuticals, Inc. Targeting apolipoprotein b for the reduction of apolipoprotein c-iii
US8409802B2 (en) 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
EP3733870A3 (de) 2011-01-14 2021-01-27 Life Technologies Corporation Verfahren zur identifikation und quantifizierung von mirnas
AT512416B1 (de) * 2012-02-13 2013-10-15 Greiner Bio One Gmbh Anordnung und verfahren zum nachweis von mikroorganismen in einem kulturgefäss
WO2015027135A1 (en) 2013-08-23 2015-02-26 Ludwig Institute For Cancer Research Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction
JP2016538882A (ja) 2013-10-30 2016-12-15 グリーン ライフ バイオテック,エルエルシー カンキツグリーニング病のための病因定量システム及び治療方法
WO2015085194A1 (en) * 2013-12-06 2015-06-11 The Broad Institute, Inc. Enhanced methods of ribonucleic acid hybridization
US9637791B2 (en) 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
WO2017216270A1 (de) 2016-06-15 2017-12-21 Grabmayr Heinrich Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
CN116264827A (zh) 2020-07-24 2023-06-16 加利福尼亚大学董事会 抗病毒前药、药物制剂和方法
EP4444904A1 (de) 2021-12-09 2024-10-16 F. Hoffmann-La Roche AG Massenbasierter nachweis von pcr-amplikons

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8311905D0 (en) * 1983-04-29 1983-06-02 Cox R A Isolation and detection of nucleotide sequence
DK51092D0 (da) * 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
ES2103918T3 (es) 1991-10-17 1997-10-01 Ciba Geigy Ag Nucleosidos biciclicos, oligonucleotidos, procedimiento para su obtencion y productos intermedios.
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5821060A (en) * 1996-08-02 1998-10-13 Atom Sciences, Inc. DNA sequencing, mapping, and diagnostic processes using hybridization chips and unlabeled DNA
ATE232880T1 (de) 1996-11-18 2003-03-15 Takeshi Imanishi Neue nucleotidanaloga
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
DE69829760T3 (de) 1997-09-12 2016-04-14 Exiqon A/S Bi- und tri-zyklische - nukleosid, nukleotid und oligonukleotid-analoga
US6077673A (en) * 1998-03-31 2000-06-20 Clontech Laboratories, Inc. Mouse arrays and kits comprising the same

Also Published As

Publication number Publication date
EP1161561A1 (de) 2001-12-12
DE60025172D1 (de) 2006-02-02
IL145422A0 (en) 2002-06-30
CA2366231A1 (en) 2000-09-28
EP1161561B1 (de) 2005-12-28
WO2000056920A1 (en) 2000-09-28
US20030039974A1 (en) 2003-02-27
US6303315B1 (en) 2001-10-16
JP2002539802A (ja) 2002-11-26
AU3274600A (en) 2000-10-09
KR20020007332A (ko) 2002-01-26
JP4975905B2 (ja) 2012-07-11
CA2366231C (en) 2010-05-11
DE60025172T2 (de) 2006-09-21
CN1350591A (zh) 2002-05-22

Similar Documents

Publication Publication Date Title
ATE314488T1 (de) Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben
KR102088381B1 (ko) 압타머-기반 다중 검정법
US10308929B2 (en) Method of preparing biological material
ATE493508T1 (de) Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren
WO2003006650A1 (en) Method of purifying nucleic acid using nonwoven fabric and detection method
JP2004509648A (ja) 非病原性又は病原性インフルエンザaサブタイプh5ウイルス検出キット
Jung et al. Reversal of RT-PCR inhibition observed in heparinized clinical specimens
JP7331038B2 (ja) 溶血試薬
EP3739062B1 (de) Lyselösung roter blutkörperchen
CA2422956A1 (en) Compositions and methods for detecting human immunodeficiency virus 2 (hiv-2)
Uryu et al. Research note: Determination of the sex of chickens by a biotin-labeled deoxyribonucleic acid probe
EP2235208B1 (de) Verfahren zum nachweis von cryptosporidium
CN111484992B (zh) 从含有甲醛的液基细胞学防腐剂中的样本分离核酸的方法
KR102087122B1 (ko) 혈관 내피 세포주를 이용한 2.5 마이크로미터 이하 미세먼지(pm2.5) 노출에 따른 염증 반응 관련 유전자 발현 여부 확인용 바이오마커 및 이를 이용한 확인 방법
CN117126925B (zh) 一种能够长期稳定保存的Crispr-Cas核酸检测试剂盒
US7858320B2 (en) Method, substances and kits for labeling population of RNA and other substances containing vicinal diols
JPH07298897A (ja) 2つの標識プライマーを用いた標的核酸の生成、増幅及び検出方法並びにそれらを含有するpcrキット
Tran Co-extraction methods of DNA and total RNA for body fluid identification: A review
WO2001038587A3 (en) Energy-transfer assay for polynucleic acid polymerases
Bao et al. Re-use of a stripped cDNA microarray
HK1204341B (en) Aptamer-based multiplexed assays
HK1178567A1 (en) Nucleic acid isolation using polidocanol and derivatives

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties