ATE314488T1 - Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben - Google Patents
Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen probenInfo
- Publication number
- ATE314488T1 ATE314488T1 AT00910584T AT00910584T ATE314488T1 AT E314488 T1 ATE314488 T1 AT E314488T1 AT 00910584 T AT00910584 T AT 00910584T AT 00910584 T AT00910584 T AT 00910584T AT E314488 T1 ATE314488 T1 AT E314488T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- nucleic acids
- sample preparation
- biological samples
- methods
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
- C07K14/003—Peptide-nucleic acids (PNAs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199900384 | 1999-03-18 | ||
| PCT/DK2000/000128 WO2000056920A1 (en) | 1999-03-18 | 2000-03-17 | One step sample preparation and detection of nucleic acids in complex biological samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE314488T1 true ATE314488T1 (de) | 2006-01-15 |
Family
ID=8092891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00910584T ATE314488T1 (de) | 1999-03-18 | 2000-03-17 | Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US6303315B1 (de) |
| EP (1) | EP1161561B1 (de) |
| JP (1) | JP4975905B2 (de) |
| KR (1) | KR20020007332A (de) |
| CN (1) | CN1350591A (de) |
| AT (1) | ATE314488T1 (de) |
| AU (1) | AU3274600A (de) |
| CA (1) | CA2366231C (de) |
| DE (1) | DE60025172T2 (de) |
| IL (1) | IL145422A0 (de) |
| WO (1) | WO2000056920A1 (de) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
| US6436640B1 (en) * | 1999-03-18 | 2002-08-20 | Exiqon A/S | Use of LNA in mass spectrometry |
| US6248535B1 (en) * | 1999-12-20 | 2001-06-19 | University Of Southern California | Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens |
| AU2001247414A1 (en) | 2000-03-14 | 2001-09-24 | Active Motif | Oligonucleotide analogues, methods of synthesis and methods of use |
| US6962906B2 (en) | 2000-03-14 | 2005-11-08 | Active Motif | Oligonucleotide analogues, methods of synthesis and methods of use |
| AU2001282920A1 (en) * | 2000-07-19 | 2002-01-30 | Genisphere Inc | Methods for detecting and assaying nucleic acid sequences |
| US20020022231A1 (en) * | 2000-08-18 | 2002-02-21 | Vaughn Vasil | Method for identifying modulators of DNA structure-specific binding proteins by high-throughput screening |
| US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| US7049059B2 (en) * | 2000-12-01 | 2006-05-23 | Response Genetics, Inc. | Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression |
| US20030077609A1 (en) * | 2001-03-25 | 2003-04-24 | Jakobsen Mogens Havsteen | Modified oligonucleotides and uses thereof |
| GB0114852D0 (en) * | 2001-06-18 | 2001-08-08 | Medical Res Council | Happiar mapping |
| US7407943B2 (en) * | 2001-08-01 | 2008-08-05 | Isis Pharmaceuticals, Inc. | Antisense modulation of apolipoprotein B expression |
| US7888324B2 (en) | 2001-08-01 | 2011-02-15 | Genzyme Corporation | Antisense modulation of apolipoprotein B expression |
| AU2002332778A1 (en) * | 2001-08-31 | 2003-03-18 | Datascope Investment Corp. | Methods for blocking nonspecific hybridizations of nucleic acid sequences |
| DE10152925A1 (de) * | 2001-10-26 | 2003-05-08 | Febit Ag | Asymmetrische Sonden |
| JP2003199568A (ja) * | 2002-01-10 | 2003-07-15 | Nichirei Corp | Dna増幅反応の効率向上方法 |
| DE10208794A1 (de) * | 2002-02-28 | 2003-09-04 | Degussa Bioactives Gmbh | Verfahren zum Sortieren einsträngiger Nucleinsäuren |
| EP1527175B1 (de) * | 2002-06-24 | 2009-05-27 | Exiqon A/S | Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen |
| US20040018634A1 (en) * | 2002-07-29 | 2004-01-29 | Kiamars Hajizadeh | Sample-collection and preparation device and method |
| US20040018120A1 (en) * | 2002-07-29 | 2004-01-29 | Craig Rappin | Sample preparation device and method |
| US20040018575A1 (en) * | 2002-07-29 | 2004-01-29 | Craig Rappin | Sample preparation device and method |
| EP1546404A2 (de) * | 2002-09-11 | 2005-06-29 | Exiqon A/S | Nukleinsäurepopulation mit teilpopulation aus lna-oligomeren |
| US20040219565A1 (en) * | 2002-10-21 | 2004-11-04 | Sakari Kauppinen | Oligonucleotides useful for detecting and analyzing nucleic acids of interest |
| US7511131B2 (en) | 2002-11-13 | 2009-03-31 | Genzyme Corporation | Antisense modulation of apolipoprotein B expression |
| JP4437544B2 (ja) | 2003-02-05 | 2010-03-24 | 株式会社半導体エネルギー研究所 | 半導体装置の作製方法 |
| CN1580283A (zh) * | 2003-08-13 | 2005-02-16 | 清华大学 | 一种检测核酸分子的方法 |
| US7897378B2 (en) | 2004-03-18 | 2011-03-01 | Roche Molecular Systems, Inc. | Method and device for purifying nucleic acids |
| US20050287558A1 (en) | 2004-05-05 | 2005-12-29 | Crooke Rosanne M | SNPs of apolipoprotein B and modulation of their expression |
| US20060014183A1 (en) * | 2004-06-10 | 2006-01-19 | Pfundheller Henrik M | Extendable probes |
| US20090118132A1 (en) * | 2004-11-04 | 2009-05-07 | Roche Molecular Systems, Inc. | Classification of Acute Myeloid Leukemia |
| US7842794B2 (en) | 2004-12-17 | 2010-11-30 | Roche Molecular Systems, Inc. | Reagents and methods for detecting Neisseria gonorrhoeae |
| US20060234277A1 (en) * | 2005-03-31 | 2006-10-19 | Amgen Inc. | Method for selectively blocking hemoglobin RNA amplification |
| US20070072211A1 (en) | 2005-06-30 | 2007-03-29 | Roche Molecular Systems, Inc. | Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids |
| US20070072223A1 (en) * | 2005-09-16 | 2007-03-29 | Primera Biosystems, Inc. | Compositions and methods for purifying nucleic acids |
| WO2007131237A2 (en) | 2006-05-05 | 2007-11-15 | Isis Pharmaceuticals, Inc. | Compounds and methods for modulating expression of ptp1b |
| DE102006031764B4 (de) * | 2006-07-06 | 2009-10-01 | Aj Innuscreen Gmbh | Verfahren zur parallelen Isolierung doppel- und einzelsträngiger Nukleinsäuren sowie zur selektiven Entfernung doppelsträngiger Nukleinsäuren aus einem Gemisch von doppel- und einzelsträngigen Nukleinsäuren |
| CA2659632A1 (en) * | 2006-08-01 | 2008-02-07 | Applera Corporation | Detection of analytes and nucleic acids |
| CN103173346B (zh) * | 2006-11-06 | 2017-04-19 | 科隆迪亚戈有限公司 | 使用结合元件用于分析的装置和方法 |
| US8293684B2 (en) * | 2006-11-29 | 2012-10-23 | Exiqon | Locked nucleic acid reagents for labelling nucleic acids |
| GB0625595D0 (en) * | 2006-12-21 | 2007-01-31 | Oxford Gene Tech Ip Ltd | Sample analyser |
| KR20150090284A (ko) | 2007-03-24 | 2015-08-05 | 젠자임 코포레이션 | 인간 아포리포프로틴 b에 상보적인 안티센스 올리고뉴클레오타이드 투여 |
| US20100331534A1 (en) * | 2007-07-27 | 2010-12-30 | Ge Healthcare Bio-Sciences Corp. | nucleic acid purification method |
| US10150990B2 (en) | 2008-04-21 | 2018-12-11 | Roche Molecular Systems, Inc. | Ribonucleotide tag nucleic acid detection |
| WO2010107838A1 (en) | 2009-03-16 | 2010-09-23 | Isis Pharmaceuticals, Inc. | Targeting apolipoprotein b for the reduction of apolipoprotein c-iii |
| US8409802B2 (en) | 2009-08-14 | 2013-04-02 | Roche Molecular Systems, Inc. | Format of probes to detect nucleic acid differences |
| EP3733870A3 (de) | 2011-01-14 | 2021-01-27 | Life Technologies Corporation | Verfahren zur identifikation und quantifizierung von mirnas |
| AT512416B1 (de) * | 2012-02-13 | 2013-10-15 | Greiner Bio One Gmbh | Anordnung und verfahren zum nachweis von mikroorganismen in einem kulturgefäss |
| CN105579587A (zh) | 2013-08-23 | 2016-05-11 | 惠氏公司 | 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 |
| US10351918B2 (en) | 2013-10-30 | 2019-07-16 | Green Life Biotech, Llc | Pathogenesis quantification systems and treatment methods for citrus greening blight |
| WO2015085194A1 (en) * | 2013-12-06 | 2015-06-11 | The Broad Institute, Inc. | Enhanced methods of ribonucleic acid hybridization |
| US9637791B2 (en) | 2013-12-20 | 2017-05-02 | Roche Molecular Systems, Inc. | Multiplexed nucleic acid target identification by strucutre based probe cleavage |
| EP3472351B1 (de) | 2016-06-15 | 2020-10-28 | Ludwig-Maximilians-Universität München | Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie |
| US12173029B2 (en) | 2020-07-24 | 2024-12-24 | The Regents Of The University Of California | Antiviral prodrugs, pharmaceutical formulations, and methods |
| WO2023104812A1 (en) | 2021-12-09 | 2023-06-15 | F. Hoffmann-La Roche Ag | Mass based detection of pcr amplicons |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8311905D0 (en) * | 1983-04-29 | 1983-06-02 | Cox R A | Isolation and detection of nucleotide sequence |
| DK51092D0 (da) * | 1991-05-24 | 1992-04-15 | Ole Buchardt | Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf |
| EP0538194B1 (de) | 1991-10-17 | 1997-06-04 | Novartis AG | Bicyclische Nukleoside, Oligonukleotide, Verfahren zu deren Herstellung und Zwischenprodukte |
| US5942391A (en) * | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
| US5821060A (en) * | 1996-08-02 | 1998-10-13 | Atom Sciences, Inc. | DNA sequencing, mapping, and diagnostic processes using hybridization chips and unlabeled DNA |
| DE69719220T2 (de) | 1996-11-18 | 2004-01-22 | Takeshi Imanishi | Neue nucleotidanaloga |
| JP3756313B2 (ja) | 1997-03-07 | 2006-03-15 | 武 今西 | 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体 |
| AU9063398A (en) | 1997-09-12 | 1999-04-05 | Exiqon A/S | Oligonucleotide analogues |
| US6077673A (en) * | 1998-03-31 | 2000-06-20 | Clontech Laboratories, Inc. | Mouse arrays and kits comprising the same |
-
2000
- 2000-03-17 AU AU32746/00A patent/AU3274600A/en not_active Abandoned
- 2000-03-17 WO PCT/DK2000/000128 patent/WO2000056920A1/en not_active Ceased
- 2000-03-17 JP JP2000606779A patent/JP4975905B2/ja not_active Expired - Lifetime
- 2000-03-17 IL IL14542200A patent/IL145422A0/xx unknown
- 2000-03-17 KR KR1020017011770A patent/KR20020007332A/ko not_active Withdrawn
- 2000-03-17 EP EP00910584A patent/EP1161561B1/de not_active Expired - Lifetime
- 2000-03-17 CA CA2366231A patent/CA2366231C/en not_active Expired - Lifetime
- 2000-03-17 AT AT00910584T patent/ATE314488T1/de not_active IP Right Cessation
- 2000-03-17 DE DE60025172T patent/DE60025172T2/de not_active Expired - Lifetime
- 2000-03-17 CN CN00807333A patent/CN1350591A/zh active Pending
- 2000-03-18 US US09/528,271 patent/US6303315B1/en not_active Expired - Lifetime
-
2001
- 2001-08-07 US US09/924,366 patent/US20030039974A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE60025172T2 (de) | 2006-09-21 |
| DE60025172D1 (de) | 2006-02-02 |
| CA2366231A1 (en) | 2000-09-28 |
| AU3274600A (en) | 2000-10-09 |
| US6303315B1 (en) | 2001-10-16 |
| WO2000056920A1 (en) | 2000-09-28 |
| IL145422A0 (en) | 2002-06-30 |
| JP4975905B2 (ja) | 2012-07-11 |
| CN1350591A (zh) | 2002-05-22 |
| US20030039974A1 (en) | 2003-02-27 |
| EP1161561B1 (de) | 2005-12-28 |
| CA2366231C (en) | 2010-05-11 |
| KR20020007332A (ko) | 2002-01-26 |
| EP1161561A1 (de) | 2001-12-12 |
| JP2002539802A (ja) | 2002-11-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |