DE60025172D1 - Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben - Google Patents

Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben

Info

Publication number
DE60025172D1
DE60025172D1 DE60025172T DE60025172T DE60025172D1 DE 60025172 D1 DE60025172 D1 DE 60025172D1 DE 60025172 T DE60025172 T DE 60025172T DE 60025172 T DE60025172 T DE 60025172T DE 60025172 D1 DE60025172 D1 DE 60025172D1
Authority
DE
Germany
Prior art keywords
nucleic acid
nucleic acids
sample preparation
methods
lna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60025172T
Other languages
English (en)
Other versions
DE60025172T2 (de
Inventor
Jan Skouv
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exiqon AS
Original Assignee
Exiqon AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exiqon AS filed Critical Exiqon AS
Publication of DE60025172D1 publication Critical patent/DE60025172D1/de
Application granted granted Critical
Publication of DE60025172T2 publication Critical patent/DE60025172T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C07K14/003—Peptide-nucleic acids (PNAs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60025172T 1999-03-18 2000-03-17 Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben Expired - Lifetime DE60025172T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK9900384 1999-03-18
DKPA199900384 1999-03-18
PCT/DK2000/000128 WO2000056920A1 (en) 1999-03-18 2000-03-17 One step sample preparation and detection of nucleic acids in complex biological samples

Publications (2)

Publication Number Publication Date
DE60025172D1 true DE60025172D1 (de) 2006-02-02
DE60025172T2 DE60025172T2 (de) 2006-09-21

Family

ID=8092891

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60025172T Expired - Lifetime DE60025172T2 (de) 1999-03-18 2000-03-17 Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben

Country Status (11)

Country Link
US (2) US6303315B1 (de)
EP (1) EP1161561B1 (de)
JP (1) JP4975905B2 (de)
KR (1) KR20020007332A (de)
CN (1) CN1350591A (de)
AT (1) ATE314488T1 (de)
AU (1) AU3274600A (de)
CA (1) CA2366231C (de)
DE (1) DE60025172T2 (de)
IL (1) IL145422A0 (de)
WO (1) WO2000056920A1 (de)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6436640B1 (en) * 1999-03-18 2002-08-20 Exiqon A/S Use of LNA in mass spectrometry
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
CA2402319A1 (en) 2000-03-14 2001-09-20 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
US6962906B2 (en) 2000-03-14 2005-11-08 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
EP1319179B1 (de) * 2000-07-19 2008-03-05 Genisphere Inc. Verfahren zur erkennung und zum assay von nukleinsäuresequenzen
US20020022231A1 (en) * 2000-08-18 2002-02-21 Vaughn Vasil Method for identifying modulators of DNA structure-specific binding proteins by high-throughput screening
US7049059B2 (en) * 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
EP1247815A3 (de) * 2001-03-25 2003-01-29 Exiqon A/S Modifizierte Oligonukleotide sowie deren Verwendung
GB0114852D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Happiar mapping
US7407943B2 (en) 2001-08-01 2008-08-05 Isis Pharmaceuticals, Inc. Antisense modulation of apolipoprotein B expression
US7888324B2 (en) 2001-08-01 2011-02-15 Genzyme Corporation Antisense modulation of apolipoprotein B expression
JP2005502346A (ja) * 2001-08-31 2005-01-27 データスコープ・インベストメント・コーポレイション 核酸配列の非特異的ハイブリダイゼーションをブロックするための方法
DE10152925A1 (de) * 2001-10-26 2003-05-08 Febit Ag Asymmetrische Sonden
JP2003199568A (ja) * 2002-01-10 2003-07-15 Nichirei Corp Ｄｎａ増幅反応の効率向上方法
DE10208794A1 (de) * 2002-02-28 2003-09-04 Degussa Bioactives Gmbh Verfahren zum Sortieren einsträngiger Nucleinsäuren
DE60327775D1 (de) * 2002-06-24 2009-07-09 Exiqon As Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen
US20040018575A1 (en) * 2002-07-29 2004-01-29 Craig Rappin Sample preparation device and method
US20040018120A1 (en) * 2002-07-29 2004-01-29 Craig Rappin Sample preparation device and method
US20040018634A1 (en) * 2002-07-29 2004-01-29 Kiamars Hajizadeh Sample-collection and preparation device and method
AU2003260292A1 (en) * 2002-09-11 2004-04-30 Exiqon A/S A population of nucleic acids including a subpopulation of lna oligomers
US20040219565A1 (en) * 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
US7511131B2 (en) 2002-11-13 2009-03-31 Genzyme Corporation Antisense modulation of apolipoprotein B expression
JP4437544B2 (ja) 2003-02-05 2010-03-24 株式会社半導体エネルギー研究所 半導体装置の作製方法
CN1580283A (zh) * 2003-08-13 2005-02-16 清华大学 一种检测核酸分子的方法
US7897378B2 (en) 2004-03-18 2011-03-01 Roche Molecular Systems, Inc. Method and device for purifying nucleic acids
US20050287558A1 (en) 2004-05-05 2005-12-29 Crooke Rosanne M SNPs of apolipoprotein B and modulation of their expression
US20060014183A1 (en) * 2004-06-10 2006-01-19 Pfundheller Henrik M Extendable probes
US20090118132A1 (en) * 2004-11-04 2009-05-07 Roche Molecular Systems, Inc. Classification of Acute Myeloid Leukemia
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
WO2006105487A1 (en) * 2005-03-31 2006-10-05 Amgen Inc. Method for selectively blocking hemoglobin rna amplification
US7919242B2 (en) 2005-06-30 2011-04-05 Roche Molecular Systems, Inc. Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
CA2622712A1 (en) * 2005-09-16 2007-03-29 Primera Biosystems, Inc. Compositions and methods for purifying nucleic acids
CN103554205A (zh) 2006-05-05 2014-02-05 Isis制药公司 调节gccr的表达的化合物和方法
DE102006031764B4 (de) * 2006-07-06 2009-10-01 Aj Innuscreen Gmbh Verfahren zur parallelen Isolierung doppel- und einzelsträngiger Nukleinsäuren sowie zur selektiven Entfernung doppelsträngiger Nukleinsäuren aus einem Gemisch von doppel- und einzelsträngigen Nukleinsäuren
EP2484781B1 (de) * 2006-08-01 2017-08-23 Applied Biosystems, LLC Nachweis von Analyten und Nukleinsäuren
CA2668639C (en) * 2006-11-06 2017-06-27 Clondiag Gmbh Assays
US8293684B2 (en) * 2006-11-29 2012-10-23 Exiqon Locked nucleic acid reagents for labelling nucleic acids
GB0625595D0 (en) * 2006-12-21 2007-01-31 Oxford Gene Tech Ip Ltd Sample analyser
JP2010522245A (ja) 2007-03-24 2010-07-01 ゲンザイム コーポレイション ヒトアポリポタンパク質ｂと相補的なアンチセンスオリゴヌクレオチドの投与
WO2009018034A1 (en) * 2007-07-27 2009-02-05 Ge Healthcare Bio-Sciences Corp. An improved nucleic acid purification method
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
US9107933B2 (en) 2009-03-16 2015-08-18 Isis Pharmaceuticals, Inc. Compositions and methods of targeting apolipoprotein B for the reduction of apolipoprotein C-III
US8409802B2 (en) 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
US20120208189A1 (en) 2011-01-14 2012-08-16 Life Technologies Corporation Methods for isolation, identification, and quantification of mirnas
AT512416B1 (de) * 2012-02-13 2013-10-15 Greiner Bio One Gmbh Anordnung und verfahren zum nachweis von mikroorganismen in einem kulturgefäss
US10266894B2 (en) 2013-08-23 2019-04-23 Ludwig Institute For Cancer Research Ltd Methods and compositions for cDNA synthesis and single-cell transcriptome profiling using template switching reaction
EP3063272A2 (de) 2013-10-30 2016-09-07 Green Life Biotech, LLC Pathogenesequantifizierungssysteme und behandlungsverfahren für mehltau von zitrusgewächsen
US10253353B2 (en) 2013-12-06 2019-04-09 The Broad Institute, Inc. Enhanced methods of ribonucleic acid hybridization
US9637791B2 (en) 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
WO2023104812A1 (en) 2021-12-09 2023-06-15 F. Hoffmann-La Roche Ag Mass based detection of pcr amplicons

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8311905D0 (en) * 1983-04-29 1983-06-02 Cox R A Isolation and detection of nucleotide sequence
DK51092D0 (da) * 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
ES2103918T3 (es) 1991-10-17 1997-10-01 Ciba Geigy Ag Nucleosidos biciclicos, oligonucleotidos, procedimiento para su obtencion y productos intermedios.
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5821060A (en) * 1996-08-02 1998-10-13 Atom Sciences, Inc. DNA sequencing, mapping, and diagnostic processes using hybridization chips and unlabeled DNA
US6043060A (en) 1996-11-18 2000-03-28 Imanishi; Takeshi Nucleotide analogues
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
EP2341058A3 (de) 1997-09-12 2011-11-23 Exiqon A/S Oligonukleotidanaloga
US6077673A (en) * 1998-03-31 2000-06-20 Clontech Laboratories, Inc. Mouse arrays and kits comprising the same

Also Published As

Publication number Publication date
CA2366231A1 (en) 2000-09-28
EP1161561A1 (de) 2001-12-12
KR20020007332A (ko) 2002-01-26
CN1350591A (zh) 2002-05-22
US20030039974A1 (en) 2003-02-27
IL145422A0 (en) 2002-06-30
EP1161561B1 (de) 2005-12-28
ATE314488T1 (de) 2006-01-15
JP2002539802A (ja) 2002-11-26
DE60025172T2 (de) 2006-09-21
CA2366231C (en) 2010-05-11
US6303315B1 (en) 2001-10-16
WO2000056920A1 (en) 2000-09-28
JP4975905B2 (ja) 2012-07-11
AU3274600A (en) 2000-10-09

Similar Documents

Publication Publication Date Title
DE60025172D1 (de) Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben
KR102088381B1 (ko) 압타머-기반 다중 검정법
Osman et al. Application of a spotting sample preparation technique for the detection of pathogens in woody plants by RT-PCR and real-time PCR (TaqMan)
O'Guinn et al. Field detection of eastern equine encephalitis virus in the Amazon Basin region of Peru using reverse transcription-polymerase chain reaction adapted for field identification of arthropod-borne pathogens
ATE493508T1 (de) Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren
US20140370508A1 (en) A method of preparing biological material
CA2614069C (en) Nucleic acid isolation using polidocanol and derivatives
WO2002055985A3 (en) Methods and reagents for identifying rare fetal cells in the material circulation
JP2012191943A (ja) 非病原性又は病原性インフルエンザａサブタイプｈ５ウイルス検出キット
AU2002214936A1 (en) A kit for detecting non-pathogenic or pathogenic influenza a subtype H5 virus
CN107893100A (zh) 一种单细胞mRNA逆转录与扩增的方法
JP2021121809A (ja) 溶血試薬
CN112852921A (zh) 一种基于即时检测试纸条的核酸检测方法、检测探针及其试剂盒
EP1932913A1 (de) Nukleinsäureisolation mithilfe von Polidocanol und Derivaten davon
EP3239710A1 (de) Verfahren zur trennung von biotinylierter nukleinsäure
JP6767374B2 (ja) 赤血球溶解溶液
AU2008338240B2 (en) A method of detecting cryptosporidium
CN102115739A (zh) 分离核酸的方法、试剂及套组
KR20190127350A (ko) 혈관 내피 세포주를 이용한 2.5 마이크로미터 이하 미세먼지(pm2.5) 노출에 따른 염증 반응 관련 유전자 발현 여부 확인용 바이오마커 및 이를 이용한 확인 방법
CN103571825A (zh) 用于生物样本处理之组合物及使用其之核酸扩增方法
CN111484992B (zh) 从含有甲醛的液基细胞学防腐剂中的样本分离核酸的方法
US7858320B2 (en) Method, substances and kits for labeling population of RNA and other substances containing vicinal diols
CN117126925B (zh) 一种能够长期稳定保存的Crispr-Cas核酸检测试剂盒
Tran et al. Co-extraction methods of DNA and total RNA for body fluid identification: A review
WO2017057076A1 (ja) 有核赤血球の取得方法および有核赤血球の識別方法

Legal Events

Date Code Title Description
8364 No opposition during term of opposition