AU2001282920A1 - Methods for detecting and assaying nucleic acid sequences - Google Patents

Methods for detecting and assaying nucleic acid sequences

Info

Publication number
AU2001282920A1
AU2001282920A1 AU2001282920A AU8292001A AU2001282920A1 AU 2001282920 A1 AU2001282920 A1 AU 2001282920A1 AU 2001282920 A AU2001282920 A AU 2001282920A AU 8292001 A AU8292001 A AU 8292001A AU 2001282920 A1 AU2001282920 A1 AU 2001282920A1
Authority
AU
Australia
Prior art keywords
detecting
methods
nucleic acid
acid sequences
assaying nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001282920A
Inventor
Robert C. Getts
James M. Kadushin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genisphere Inc
Original Assignee
Genisphere Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genisphere Inc filed Critical Genisphere Inc
Publication of AU2001282920A1 publication Critical patent/AU2001282920A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is directed to a method for determining the presence of a specific nucleotide sequence in an RNA reagent of a target sample utilizing a capture reagent having at least one first arm containing a label capable of emitting a detectable signal and at least one second arm having a nucleotide sequence complementary to a capture sequence attached to the RNA reagent on a microarray.
AU2001282920A 2000-07-19 2001-07-19 Methods for detecting and assaying nucleic acid sequences Abandoned AU2001282920A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21939700P 2000-07-19 2000-07-19
US60219397 2000-07-19
PCT/US2001/022818 WO2002006511A2 (en) 2000-07-19 2001-07-19 Methods for detecting and assaying nucleic acid sequences

Publications (1)

Publication Number Publication Date
AU2001282920A1 true AU2001282920A1 (en) 2002-01-30

Family

ID=22819100

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001282920A Abandoned AU2001282920A1 (en) 2000-07-19 2001-07-19 Methods for detecting and assaying nucleic acid sequences

Country Status (7)

Country Link
US (1) US20020072060A1 (en)
EP (1) EP1319179B1 (en)
JP (1) JP2004506179A (en)
AT (1) ATE388402T1 (en)
AU (1) AU2001282920A1 (en)
DE (1) DE60133111T2 (en)
WO (1) WO2002006511A2 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6545127B1 (en) 1999-06-28 2003-04-08 Oklahoma Medical Research Foundation Catalytically active recombinant memapsin and methods of use thereof
CA2400680A1 (en) * 2000-03-08 2001-09-13 Datascope Investment Corp. Methods for assay and detection on a microarray
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US20040265883A1 (en) * 2003-06-27 2004-12-30 Biocept, Inc. mRNA expression analysis
US20050084880A1 (en) * 2003-07-11 2005-04-21 Ronald Duman Systems and methods for diagnosing & treating psychological and behavioral conditions
JP4996248B2 (en) 2003-07-31 2012-08-08 ハンディーラブ インコーポレイテッド Processing of particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
EP1769088A2 (en) * 2004-06-30 2007-04-04 Whitehead Institute For Biomedical Research Novel methods for high-throughput genome-wide location analysis
CN1312293C (en) * 2004-11-08 2007-04-25 博奥生物有限公司 High flux biochip and application thereof
US20070048741A1 (en) * 2005-08-24 2007-03-01 Getts Robert C Methods and kits for sense RNA synthesis
US20070105124A1 (en) * 2005-11-08 2007-05-10 Getts Robert C Methods and kits for nucleic acid amplification
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
DK2001990T3 (en) 2006-03-24 2016-10-03 Handylab Inc Integrated microfluidic sample processing system and method for its use
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8765076B2 (en) 2006-11-14 2014-07-01 Handylab, Inc. Microfluidic valve and method of making same
AU2008257419B2 (en) * 2007-05-23 2013-10-24 The Trustees Of The University Of Pennsylvania Targeted carriers for intracellular drug delivery
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
AU2008276211B2 (en) * 2007-07-13 2015-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
CN106190806B (en) 2011-04-15 2018-11-06 贝克顿·迪金森公司 Scan real-time microfluid thermal cycler and the method for synchronous thermal cycle and scanning optical detection
KR102121853B1 (en) 2011-09-30 2020-06-12 벡톤 디킨슨 앤드 컴퍼니 Unitized reagent strip
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
CN104040238B (en) 2011-11-04 2017-06-27 汉迪拉布公司 Polynucleotides sample preparation apparatus
AU2013214849B2 (en) 2012-02-03 2016-09-01 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US9651549B2 (en) * 2012-07-13 2017-05-16 Genisphere, Llc Lateral flow assays using DNA dendrimers

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE23752T1 (en) * 1980-08-21 1986-12-15 Oriel Scient Ltd OPTICAL ANALYZER.
US5175270A (en) * 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
WO1997027317A1 (en) * 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
US6361940B1 (en) * 1996-09-24 2002-03-26 Qiagen Genomics, Inc. Compositions and methods for enhancing hybridization and priming specificity
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
ATE327344T1 (en) * 1997-07-29 2006-06-15 Polyprobe Inc DENDRITIC NUCLEIC ACIDS WITH MAXIMUM SELF-COLLECTIVE STORAGE
US6261779B1 (en) * 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
JP4975905B2 (en) * 1999-03-18 2012-07-11 エクシコン エ/エス Single-step preparation and detection of nucleic acids in complex biological samples
CA2400680A1 (en) * 2000-03-08 2001-09-13 Datascope Investment Corp. Methods for assay and detection on a microarray
US20020094538A1 (en) * 2001-01-13 2002-07-18 Getts Robert C. Methods for detecting and assaying nucleic acid sequences using temperature cycling
WO2003012147A1 (en) * 2001-02-20 2003-02-13 Datascope Investment Corp. Method for reusing standard blots and microarrays utilizing dna dendrimer technology
WO2003020902A2 (en) * 2001-08-31 2003-03-13 Datascope Investment Corp. Methods for blocking nonspecific hybridizations of nucleic acid sequences

Also Published As

Publication number Publication date
ATE388402T1 (en) 2008-03-15
WO2002006511A2 (en) 2002-01-24
EP1319179A2 (en) 2003-06-18
EP1319179A4 (en) 2005-01-19
DE60133111T2 (en) 2009-03-12
US20020072060A1 (en) 2002-06-13
DE60133111D1 (en) 2008-04-17
EP1319179B1 (en) 2008-03-05
JP2004506179A (en) 2004-02-26
WO2002006511A3 (en) 2002-04-11

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