ATE432347T1 - Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen - Google Patents

Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen

Info

Publication number
ATE432347T1
ATE432347T1 AT03780549T AT03780549T ATE432347T1 AT E432347 T1 ATE432347 T1 AT E432347T1 AT 03780549 T AT03780549 T AT 03780549T AT 03780549 T AT03780549 T AT 03780549T AT E432347 T1 ATE432347 T1 AT E432347T1
Authority
AT
Austria
Prior art keywords
nucleic acid
isolation
molecules
rna
nucleotide sequence
Prior art date
Application number
AT03780549T
Other languages
English (en)
Inventor
Sakari Kauppinen
Nana Jacobsen
Original Assignee
Exiqon As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exiqon As filed Critical Exiqon As
Application granted granted Critical
Publication of ATE432347T1 publication Critical patent/ATE432347T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT03780549T 2002-06-24 2003-06-20 Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen ATE432347T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39092802P 2002-06-24 2002-06-24
PCT/IB2003/006354 WO2004020575A2 (en) 2002-06-24 2003-06-20 Methods and systems for detection and isolation of a nucleotide sequence

Publications (1)

Publication Number Publication Date
ATE432347T1 true ATE432347T1 (de) 2009-06-15

Family

ID=31978221

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03780549T ATE432347T1 (de) 2002-06-24 2003-06-20 Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen

Country Status (6)

Country Link
US (2) US20050053942A1 (de)
EP (1) EP1527175B1 (de)
AT (1) ATE432347T1 (de)
AU (1) AU2003288474A1 (de)
DE (1) DE60327775D1 (de)
WO (1) WO2004020575A2 (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005011350A1 (de) * 2005-03-11 2006-09-14 Eppendorf Ag CDNA Microarrays mit Zufallsspacern
CA2622712A1 (en) * 2005-09-16 2007-03-29 Primera Biosystems, Inc. Compositions and methods for purifying nucleic acids
EP2458011B1 (de) * 2005-10-03 2013-07-24 Life Technologies Corporation Zusammensetzungen, Verfahren und Kits zur Amplifikation von Nukleinsäuren
JP2007105041A (ja) * 2005-10-13 2007-04-26 F Hoffmann La Roche Ag 多重固相核酸増幅アッセイ
EP2029777B1 (de) * 2006-05-31 2017-03-08 Sequenom, Inc. Verfahren und zusammensetzungen zur nukleinsäureextraktion aus einer probe
EP2069497B1 (de) * 2006-11-02 2013-08-14 University of Utah Research Foundation Oligonukleotide für allelspezifische pcr
WO2008150937A2 (en) * 2007-05-29 2008-12-11 Aivaradx, Inc. Real-time pcr
WO2009024781A1 (en) * 2007-08-20 2009-02-26 The General Hospital Corporation Isolation of protein factors that associate directly or indirectly with nucleic acids
EP2548962B1 (de) 2007-09-19 2016-01-13 Applied Biosystems, LLC Sirna-sequenz-unabhängige Modifikationsformate zur Verringerung von das Ziel verfehlenden phänotypischen Effekten bei RNAI und stabilisierte Formen davon
EP3964586A1 (de) 2009-04-03 2022-03-09 Sequenom, Inc. Nukleinsäurepräparatzusammensetzungen und verfahren
US12410463B2 (en) * 2009-09-18 2025-09-09 The Trustees Of The University Of Pennsylvania Molecular beacons
US8278049B2 (en) * 2010-04-26 2012-10-02 Ann & Robert H. Lurie Children's Hospital of Chicago Selective enrichment of CpG islands
US9676810B2 (en) 2010-07-08 2017-06-13 The Brigham And Women's Hospital, Inc. Neuroprotective molecules and methods of treating neurological disorders and inducing stress granules
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
US20140329700A1 (en) * 2011-08-23 2014-11-06 Rutgers, The State University Of New Jersey Methods of isolating rna and mapping of polyadenylation isoforms
WO2013078302A1 (en) 2011-11-23 2013-05-30 Cornell University Methods for diagnosing human immunodeficiency virus infections
US9725770B2 (en) * 2012-03-06 2017-08-08 The Regents Of The University Of California Methods and compositions for identification of source of microbial contamination in a sample
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP2834259A4 (de) 2012-04-02 2016-08-24 Moderna Therapeutics Inc Modifizierte polynukleotide
US10086093B2 (en) 2013-02-28 2018-10-02 The General Hospital Corporation miRNA profiling compositions and methods of use
JP2016515216A (ja) 2013-03-14 2016-05-26 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド メッセンジャーｒｎａのキャップ効率の定量的評価
WO2014152659A1 (en) 2013-03-14 2014-09-25 Shire Human Genetic Therapies, Inc. Quantitative assessment for cap efficiency of messenger rna
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
EP3578663A1 (de) 2013-03-15 2019-12-11 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
US11377470B2 (en) 2013-03-15 2022-07-05 Modernatx, Inc. Ribonucleic acid purification
CN103308373A (zh) * 2013-06-05 2013-09-18 南昌大学 磁珠分离大肠杆菌o157的方法
DK3019619T3 (da) 2013-07-11 2021-10-11 Modernatx Inc Sammensætninger, der omfatter syntetiske polynukleotider, som koder for crispr-beslægtede proteiner, og syntetiske sgrna'er, og anvendelsesfremgangsmåder
EP3052511A4 (de) 2013-10-02 2017-05-31 Moderna Therapeutics, Inc. Polynukleotidmoleküle und verwendungen davon
US10286086B2 (en) 2014-06-19 2019-05-14 Modernatx, Inc. Alternative nucleic acid molecules and uses thereof
US10407683B2 (en) 2014-07-16 2019-09-10 Modernatx, Inc. Circular polynucleotides
US10093967B2 (en) * 2014-08-12 2018-10-09 The Regents Of The University Of Michigan Detection of nucleic acids
EP3230744B1 (de) * 2014-12-12 2021-05-12 Exact Sciences Development Company, LLC Vorrichtungen und verfahren zur durchführung von methylierungserkennungstests
CN107208135B (zh) 2014-12-12 2021-10-15 精密科学公司 用于进行甲基化检测测定的组合物和方法
WO2017049286A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a morpholino linker
PL3350333T3 (pl) 2015-09-17 2022-03-07 Modernatx, Inc. Polinukleotydy zawierające region stabilizujący ogon
EP4299763A2 (de) * 2015-09-22 2024-01-03 Biocartis NV Verbesserte detektion von kurzen homopolymeren wiederholungen
WO2017068087A1 (en) * 2015-10-22 2017-04-27 Roche Innovation Center Copenhagen A/S Oligonucleotide detection method
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
EP3592768B1 (de) 2017-03-08 2024-12-25 The Regents of The University of Michigan Analytnachweis
CN108519481B (zh) * 2018-03-08 2020-10-16 捷和泰（北京）生物科技有限公司 一种改善核心抗体磁微粒化学发光免疫分析精密度的方法
JP7347849B2 (ja) 2018-04-19 2023-09-20 ファースト ライト ダイアグノスティクス， インコーポレイテッド 標的の検出
US20210332359A1 (en) 2018-09-07 2021-10-28 The General Hospital Corporation Compositions and methods for immune checkpoint inhibition
US20210349082A1 (en) * 2018-10-04 2021-11-11 First Light Diagnostics, Inc. Analysis instrument
US11578354B2 (en) * 2019-07-16 2023-02-14 Meliolabs Inc. Methods and devices for single-cell based digital high resolution melt
WO2021026040A1 (en) * 2019-08-02 2021-02-11 Shield Diagnostics Corp. Sequence specific nucleic acid enrichment methods and uses thereof
KR20230019451A (ko) * 2020-05-29 2023-02-08 매머드 바이오사이언시즈 인크. 프로그래밍 가능한 뉴클레아제 진단 장치
US20240011080A1 (en) * 2020-12-21 2024-01-11 Illumina, Inc. Compositions and methods for capturing and amplifying target polynucleotides using modified capture primers
CN114107446A (zh) * 2021-12-16 2022-03-01 福建和瑞基因科技有限公司 一种核酸的检测试剂盒及其检测方法
WO2024015792A2 (en) * 2022-07-11 2024-01-18 Rensselaer Polytechnic Institute Ligands and methods of making ligands for affinity capture on a surface of mrna in a solution

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4670379A (en) * 1984-12-19 1987-06-02 E. I. Du Pont De Nemours And Company Polynucleotide hydridization assays employing catalyzed luminescence
US5310651A (en) * 1986-01-22 1994-05-10 Institut Pasteur DNA probes of human immunodeficiency virus type 2 (HIV-2), and methods employing these probes for dectecting the presence of HIV-2
US5622820A (en) * 1988-03-10 1997-04-22 City Of Hope Method for amplification and detection of RNA and DNA sequences
US5196306A (en) * 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
US5514545A (en) * 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5804383A (en) * 1992-08-21 1998-09-08 The Regents Of The University Of California Method and assay for detection of the expression of allele-specific mutations by allele-specific in situ reverse transcriptase polymerase chain reaction
DE4317028C3 (de) * 1993-05-21 2000-06-29 Martin Busch Verfahren zur Akquisition und Auswertung von Daten in einem Kernspin-Tomographen
US6562596B1 (en) * 1993-10-06 2003-05-13 Amgen Inc. Tissue inhibitor of metalloproteinase type three (TIMP-3) composition and methods
US5955072A (en) * 1994-07-13 1999-09-21 Sankyo Company, Limited Expression systems utilizing autolyzing fusion proteins and a reducing polypeptide
GB9417593D0 (en) * 1994-09-01 1994-10-19 Secr Defence Luciferase labelling method
US5698686A (en) * 1994-10-20 1997-12-16 Arch Development Corporation Yeast telomerase compositions
CA2248084A1 (en) * 1996-03-04 1997-09-12 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
US5713358A (en) * 1996-03-26 1998-02-03 Wisconsin Alumni Research Foundation Method for producing a time-resolved series of 3D magnetic resonance angiograms during the first passage of contrast agent
US6072043A (en) * 1996-06-04 2000-06-06 Polyprobe, Inc. Optimally fluorescent oligonucleotides
US5691153A (en) * 1996-09-06 1997-11-25 Creighton University Genetic markers to detect high bone mass
US5916750A (en) * 1997-01-08 1999-06-29 Biogenex Laboratories Multifunctional linking reagents for synthesis of branched oligomers
US5873825A (en) * 1997-04-11 1999-02-23 Wisconsin Alumni Research Foundation Three dimensional digital subtraction magnetic resonance angiography with limited k-space mask
DE69829760T3 (de) * 1997-09-12 2016-04-14 Exiqon A/S Bi- und tri-zyklische - nukleosid, nukleotid und oligonukleotid-analoga
US6487435B2 (en) * 1998-04-10 2002-11-26 Wisconsin Alumni Research Foundation Magnetic resonance angiography using undersampled 3D projection imaging
HK1048639A1 (zh) * 1999-03-18 2003-04-11 埃克西库恩公司 木-lna类似物
AU3274500A (en) * 1999-03-18 2000-10-09 Exiqon A/S Detection of mutations in genes by specific lna primers
JP4975905B2 (ja) * 1999-03-18 2012-07-11 エクシコン エ／エス 複合生物試料における核酸の単工程調製及び検出
DE19924448A1 (de) * 1999-05-28 2000-12-07 Siemens Ag Verfahren zur Gewinnung von zeit- und ortsaufgelösten dreidimensionalen Datensätzen mittels der magnetischen Resonanz und Vorrichtung zur Durchführung des Verfahrens
US20030077609A1 (en) * 2001-03-25 2003-04-24 Jakobsen Mogens Havsteen Modified oligonucleotides and uses thereof
US7003343B2 (en) * 2001-03-30 2006-02-21 Cornell Research Foundation, Inc. Method and apparatus for anatomically tailored k-space sampling and recessed elliptical view ordering for bolus-enhanced 3D MR angiography

Also Published As

Publication number Publication date
AU2003288474A1 (en) 2004-03-19
WO2004020575A3 (en) 2004-12-23
EP1527175B1 (de) 2009-05-27
US20050053942A1 (en) 2005-03-10
US20080096191A1 (en) 2008-04-24
WO2004020575A2 (en) 2004-03-11
AU2003288474A8 (en) 2004-03-19
DE60327775D1 (de) 2009-07-09
EP1527175A2 (de) 2005-05-04

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