DE60040826D1 - Verfahren zur Kultivierung von Zellen - Google Patents

Verfahren zur Kultivierung von Zellen

Info

Publication number
DE60040826D1
DE60040826D1 DE60040826T DE60040826T DE60040826D1 DE 60040826 D1 DE60040826 D1 DE 60040826D1 DE 60040826 T DE60040826 T DE 60040826T DE 60040826 T DE60040826 T DE 60040826T DE 60040826 D1 DE60040826 D1 DE 60040826D1
Authority
DE
Germany
Prior art keywords
cultivating cells
cultivating
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60040826T
Other languages
English (en)
Inventor
Nobutaka Yamamoto
Akihisa Sugiyama
Satoshi Kawaminami
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Menicon Co Ltd
Original Assignee
Menicon Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Menicon Co Ltd filed Critical Menicon Co Ltd
Application granted granted Critical
Publication of DE60040826D1 publication Critical patent/DE60040826D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
DE60040826T 1999-11-26 2000-11-24 Verfahren zur Kultivierung von Zellen Expired - Lifetime DE60040826D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP33621099 1999-11-26
JP33620899 1999-11-26

Publications (1)

Publication Number Publication Date
DE60040826D1 true DE60040826D1 (de) 2009-01-02

Family

ID=26575403

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60040826T Expired - Lifetime DE60040826D1 (de) 1999-11-26 2000-11-24 Verfahren zur Kultivierung von Zellen

Country Status (3)

Country Link
US (2) US6734016B1 (de)
EP (1) EP1103601B1 (de)
DE (1) DE60040826D1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2555021A1 (en) * 2004-02-13 2005-08-25 Reprocell, Inc. Medium for preparing feeder cells for embryonic stem cells and feeder cells
GB2442271B (en) * 2006-09-27 2011-06-15 Denis Eon Solomon An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells
JP2022542247A (ja) * 2019-07-24 2022-09-30 エスエルバイジェン インコ―ポレイテッド 不死化幹細胞株の製造方法およびその用途

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4016036A (en) * 1975-11-14 1977-04-05 Massachusetts Institute Of Technology Process for serially culturing keratinocytes
US4940666A (en) * 1983-07-15 1990-07-10 University Patents, Inc. Process and defined medium for growth of human epidermal keratinocyte cells
JPS6124520A (ja) 1984-07-12 1986-02-03 Takeshi Makitsubo 腫瘍壊死因子様物質を抽出する方法
JPH0740931B2 (ja) 1986-06-04 1995-05-10 第一製薬株式会社 細胞の培養法

Also Published As

Publication number Publication date
EP1103601A1 (de) 2001-05-30
EP1103601B1 (de) 2008-11-19
US20020031828A1 (en) 2002-03-14
US6734016B1 (en) 2004-05-11

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition