DK2308976T4 - Nyt herbicidresistensgen - Google Patents

Nyt herbicidresistensgen Download PDF

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Publication number
DK2308976T4
DK2308976T4 DK10012200.1T DK10012200T DK2308976T4 DK 2308976 T4 DK2308976 T4 DK 2308976T4 DK 10012200 T DK10012200 T DK 10012200T DK 2308976 T4 DK2308976 T4 DK 2308976T4
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DK
Denmark
Prior art keywords
plant
aad
plants
herbicide
gene
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DK10012200.1T
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English (en)
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DK2308976T3 (da
Inventor
Terry R Wright
Justin M Lira
Donald J Merlo
Nicole Arnold
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Dow Agrosciences Llc
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Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of DK2308976T3 publication Critical patent/DK2308976T3/da
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Nutrition Science (AREA)
  • Insects & Arthropods (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)

Claims (18)

1. Transgen plante, der omfatter et isoleret polynukleotid, der koder for et protein, som enzymatisk nedbryder et herbicid, der er udvalgt fra gruppen bestående af et phenoxyauxin-herbicid og et aryloxyphenoxypropionat-herbicid, hvor polynukleotidet hybridiserer under stringente forhold med den fulde komplement af et nukleinsyremolekyle, der koder for SEQ ID NO:9, hvor planten eksprimerer polynukleotidet og fremstiller proteinet.
2. Plante ifølge krav 1, hvilken plante yderligere omfatter et glyphosattole-rance-karaktertræk.
3. Plante ifølge krav 1, hvilken plante yderligere omfatter et glyphosattole-rance-karaktertræk og et glufosinattolerance-karaktertræk.
4. Plante ifølge krav 1, hvilken plante yderligere omfatter et gen til tolerance over for dicamba.
5. Plante ifølge krav 1, hvilken plante yderligere omfatter et gen til tolerance over for et herbicid, som er en acetolactatsyntaseinhibitor.
6. Plante ifølge krav 1, hvilken plante yderligere omfatter et gen til tolerance over for glufosinat.
7. Plante ifølge krav 1, hvilken plante yderligere omfatter et eller flere herbicidresistensgener.
8. Plante ifølge krav 1, hvilken plante er en majsplante.
9. Transgen plante ifølge krav 1, hvor aryloxyalkanoat-herbicidet er et 2,4-D-herbicid.
10. Transgen plante ifølge krav 1, hvor proteinet omfatter aminosyresekven-sen ifølge SEQ ID NO: 9 eller en variant af SEQ ID NO: 9, hvor varianten enzymatisk nedbryder herbicidet, mindst en aminosyredeletion eller konser- veret substitution, og mindst 95 % sekvensidentitet med SEQ ID NO: 9.
11. Plante ifølge krav 1, hvor planten også er resistent over for et eller flere herbicider udvalgt fra gruppen bestående af glyphosat, imidazolinoner, glufo-sinat, sulfonylurea, bromoxynil og dicamba.
12. Plante ifølge krav 1, hvor herbicidet er udvalgt fra gruppen bestående af 2,4-dichlorphenoxy-eddikesyre, MCPA, dichlorprop, mecoprop, fluazifop, ha-loxyfop, diclofop, quizalofop, fenoxaprop, metamifop, cyhalofop og clodino-fop.
13. Plante ifølge krav 1, hvor planten yderligere omfatter et andet herbicidresistensgen.
14. Plante ifølge krav 1, hvor planten yderligere omfatter et insektresistensgen, der stammer fra en organisme udvalgt fra gruppen bestående af Bacillus thuringiensis, Photorhabdus og Xenorhabdus.
15. Plante ifølge krav 1, hvor planten yderligere omfatter et gen for et agronomisk karaktertræk udvalgt fra gruppen bestående af fungal resistens, stresstolerance, øget udbytte, forbedret olieprofil, forbedret fiberkvalitet, viral resistens, forsinket modning, kuldetolerance og salttolerance.
16. Plante ifølge krav 13, hvor det andet herbicidresistensgen gør planten resistent over for et herbicid udvalgt fra gruppen bestående af glyphosat, glu-fosinat, ALS-inhibitorer, inhibitorer af 4-hydroxyphenyl-pyruvat-dioxygenase (HPPD) og inhibitorer af protoporphyrinogenoxidase (PPO).
17. Plantedel, frø, afkom, væv eller aseksuelt propagat af planten ifølge krav 1, hvor delen, frøet, afkommet eller det aseksuelle propagat omfatter poly-nukleotidet.
18. Basisvæv fra planten ifølge krav 8.
DK10012200.1T 2004-04-30 2005-05-02 Nyt herbicidresistensgen DK2308976T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56705204P 2004-04-30 2004-04-30
EP05771746A EP1740039B1 (en) 2004-04-30 2005-05-02 Novel herbicide resistance genes

Publications (2)

Publication Number Publication Date
DK2308976T3 DK2308976T3 (da) 2013-04-29
DK2308976T4 true DK2308976T4 (da) 2017-07-03

Family

ID=35320667

Family Applications (5)

Application Number Title Priority Date Filing Date
DK10012200.1T DK2308976T4 (da) 2004-04-30 2005-05-02 Nyt herbicidresistensgen
DK05771746.4T DK1740039T3 (da) 2004-04-30 2005-05-02 Hidtil ukendte herbicidresistensgener
DK10012199.5T DK2319932T4 (da) 2004-04-30 2005-05-02 Hidtil ukendt herbicidresistensgen
DK10012201.9T DK2298901T4 (da) 2004-04-30 2005-05-02 Nye herbicidresistente gener
DK10012202.7T DK2308977T4 (da) 2004-04-30 2005-05-02 Nyt herbicidresistensgen

Family Applications After (4)

Application Number Title Priority Date Filing Date
DK05771746.4T DK1740039T3 (da) 2004-04-30 2005-05-02 Hidtil ukendte herbicidresistensgener
DK10012199.5T DK2319932T4 (da) 2004-04-30 2005-05-02 Hidtil ukendt herbicidresistensgen
DK10012201.9T DK2298901T4 (da) 2004-04-30 2005-05-02 Nye herbicidresistente gener
DK10012202.7T DK2308977T4 (da) 2004-04-30 2005-05-02 Nyt herbicidresistensgen

Country Status (19)

Country Link
US (8) US7838733B2 (da)
EP (5) EP2308977B2 (da)
JP (2) JP5285906B2 (da)
CN (2) CN102094032B (da)
AR (5) AR048724A1 (da)
AU (1) AU2005240045B2 (da)
BR (1) BRPI0509460B8 (da)
CA (2) CA2563206C (da)
CO (1) CO7141412A2 (da)
DK (5) DK2308976T4 (da)
ES (5) ES2408244T5 (da)
HK (2) HK1108562A1 (da)
MX (1) MXPA06012634A (da)
NZ (3) NZ550602A (da)
PL (5) PL2308977T5 (da)
PT (5) PT2308977E (da)
SI (7) SI2298901T2 (da)
WO (1) WO2005107437A2 (da)
ZA (1) ZA200608610B (da)

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