CN107771181A - 用以防治昆虫有害生物的组合物和方法 - Google Patents
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Abstract
提供了使用沉默元件的方法和组合物,所述沉默元件在被植物昆虫有害生物例如鞘翅目、半翅目或鳞翅目植物有害生物,包括叶甲属、瘦跗叶甲属、条跳甲属、无网蚜属、小粉虱属、茶翅蝽属、稻绿蝽属或灰翅夜蛾属植物有害生物摄取时,降低靶标序列在所述有害生物中的表达。公开了如本文公开的SEQ ID NO:1‑53或107‑254中任一项所示的不同靶标多核苷酸、或其变体和片段、或其互补序列,其中靶标有害生物中所述序列中的一种或多种的表达的减少通过昆虫不育化(即雄性和雌性不育,精子数量、产卵量和生活力的降低)和基于交配的昆虫不育技术(SIT)来防治所述有害生物和有害生物群体。还提供了包含本发明的沉默元件或者其活性变体或片段的植物、植物部分、细菌和其他宿主细胞。本摘要旨在作为搜索工具,用于在特定领域中进行搜索的目的,而不旨在限制本发明。
Description
以电子方式提交的序列表的引用
文件名为“6030WOPCT_SequenceList.txt”的序列表创建于2016年5月19日,大小为531千字节,以计算机可读形式,同时与本说明书一起提交。该序列表是本说明书的一部分并且以其全文通过引用结合在此。
发明领域
本发明总体上涉及用以防治有害生物的分子生物学和基因沉默的方法。
背景技术
植物昆虫有害生物是农业中的严重问题。植物昆虫有害生物破坏了数百万英亩的主要作物,如玉米、大豆、豌豆和棉花。每年,仅在美国,植物昆虫有害生物就造成了超过1000亿美元的作物损失。在与昆虫有害生物进行的季节性斗争中,农场主们必须施用数十亿加仑的合成杀有害生物剂来对付这些有害生物。过去采用的其他方法通过微生物或在转基因植物中表达来源于微生物的基因来提供杀昆虫活性。例如,已知芽孢杆菌属(Bacillus)微生物的某些物种对于广泛范围的昆虫有害生物具有杀有害生物活性,这些昆虫有害生物包括鳞翅目(Lepidoptera)、双翅目(Diptera)、鞘翅目(Coleoptera)、半翅目(Hemiptera)等。事实上,微生物杀有害生物剂,特别是从芽孢杆菌菌株获得的那些微生物杀有害生物剂,在农业上作为有害生物化学防治的替代方案起到重要作用。农业科学家通过对作物植物进行遗传工程改造以产生来自芽孢杆菌的杀昆虫蛋白,开发出了昆虫抗性增强的作物。例如,经遗传工程改造而产生Cry毒素的玉米和棉花植物(参见例如Aronson(2002)Cell Mol.Life Sci.[细胞与分子生命科学],59(3):417-425;Schnepf等人(1998)Microbiol.Mol.Biol.Rev.[微生物学与分子生物学综述]62(3):775-806)目前在农业中广泛应用,并给农场主提供了传统昆虫防治方法的替代方案。然而,在一些情况下,这些Bt杀昆虫蛋白可能仅保护植物免受相对较窄范围的有害生物的侵害。不断演变的昆虫抗性也提出了一个问题(Gassmann等人,(2014)PNAS[美国国家科学院院刊]111(14):5141-6)。因此,新颖的昆虫防治组合物仍然是所希望的。
发明内容
提供了采用沉默元件的方法和组合物,所述沉默元件在被植物昆虫有害生物(例如鞘翅目(Coleopteran)、半翅目(Hemiptera)或鳞翅目(Lepidopteran)植物有害生物,包括叶甲属(Diabrotica)、瘦跗叶甲属(Leptinotarsa)、条跳甲属(Phyllotreta)、无网蚜属(Acyrthosiphan)、小粉虱属(Bemisia)、茶翅蝽属(Halyomorpha)、稻绿蝽属(Nezara)或灰翅夜蛾属(Spodoptera)植物有害生物)摄取时,能够降低靶标序列在所述有害生物中的表达。在某些实施例中,所述靶标序列的表达降低能控制有害生物繁殖的能力,并且因此这些方法和组合物能够限制昆虫有害生物对植物的损害以及昆虫有害生物的扩散。本文描述了如SEQ ID NO.:1-53或107-254中所示的各种靶标多核苷酸、或者其变体或片段、或者其互补序列,以上各项调节涉及有害生物繁殖和产卵力的靶标有害生物RNA中的序列中的一个或多个的表达。因此,如SEQ ID NO.:1-53或107-254中所示的各种靶标多核苷酸、或者其变体或片段、或者其互补序列在本文所述的方法中是有用的,所述方法通过昆虫不育化和释放不育的靶标有害生物,即昆虫不育技术(“SIT”)来防治靶标有害生物。还提供了沉默元件,所述沉默元件在被有害生物摄取时,降低这些靶标多核苷酸中的一种或多种的表达水平。进一步提供了编码沉默元件的构建体和包含编码沉默元件的构建体的宿主细胞。还提供了包含这些沉默元件或者其活性变体或片段的植物、植物部分、植物细胞、细菌和其他宿主细胞。还提供了用于局部施用到有害昆虫或可能存在有害昆虫的基质上的可喷雾沉默剂的制剂。
在另一个实施例中,提供了用于防治如下植物昆虫有害生物的方法:例如鞘翅目、半翅目或鳞翅目植物有害生物,包括叶甲属、瘦跗叶甲属、条跳甲属、无网蚜属、小粉虱属、茶翅蝽属、稻绿蝽属或灰翅夜蛾属植物有害生物。所述方法包括向植物昆虫有害生物饲喂包含沉默元件的组合物,其中所述沉默元件在被有害生物摄取时,降低所述有害生物中靶标序列的水平,并且因此防治所述有害生物。进一步提供了保护植物免受植物昆虫有害生物侵害的方法。此类方法包括将所披露的沉默元件引入植物或植物部分中。当表达沉默元件的植物被有害生物摄取时,所述靶标序列的水平得以降低并且所述有害生物受到防治。
附图简要说明
图1A-1D显示了在摄取包含含有SEQ ID NO.:4的靶标核苷酸序列的dsRNA构建体的人工饲料之后,关于西方玉米根虫(“WCRW”)成虫的不育化的代表性数据。图1A显示了在13-14天内由处理组和年龄组产生的卵的总数。对于幼龄雌性组,使用了50对雄性和雌性甲虫,并且对于老龄雌性组,使用了50只交配的雌性甲虫。图1B显示了在13-14天产卵期期间,由处理组和年龄组每只雌性/天产生的卵的平均数量。该箱线图由Spotfire程序生成,其表示4个四分位数、平均值、和平均数的95%置信区间。图1C显示了各种处理对整体平均卵孵化率的影响,如图中所示。数据代表13-14天的采卵期;n=6个重复/处理/天;5-45个卵/重复,取决于天数(p<0.001)。图1D显示了在处理幼龄组雌性和雄性昆虫后8天和处理老龄组雌性昆虫后4天,WCRW甲虫成虫中的基因抑制分析。使用DV-RPS10基因作为参照和未处理的老龄甲虫作为正规化子(normalizer),从每种处理的4只单个昆虫中显示VgR的相对表达。该箱线图显示了处理组和年龄组的4个四分位数、平均值、中值和平均数的95%置信区间。
图2A-2B显示了用包含含有SEQ ID NO.:4的靶标核苷酸序列的dsRNA构建物的人工饲料喂食3龄幼虫之后,关于WCRW的不育化的代表性数据。图2A显示了每只雌性产生的平均数量和有活力的卵。对每种处理计数来自15-42只雌性甲虫成虫的卵。该箱里的数字显示了卵或有活力的卵/雌性的平均数量。该箱线图显示了针对每种处理的4个四分位数、平均值、中值和平均数的95%置信区间。对于暴露于VgR dsRNA的组,在整个研究期间有活力的卵的产量一直非常低。用VgR dsRNA处理不影响成虫羽化。由于VgR dsRNA幼虫暴露引起的甲虫成虫的死亡率可以忽略不计。图2B显示了分别在处理后第40天和第58天,4只10日龄甲虫和超过15只28日龄甲虫中的VgR基因抑制分析。通过qRTPCR的相对表达的箱线图显示了在10天龄和28天龄甲虫中针对每种处理的4个四分位数、平均值、中值和平均数的95%置信区间。将未经处理的3龄幼虫用作正规化子。
图3A-3C显示了在暴露于包含含有SEQ ID NO.:3的靶标核苷酸序列的dsRNA的人工饲料后,有关WCRW不育化和WCRW中的基因抑制的剂量响应的数据。图3A显示了响应于VgRdsRNA剂量在18天研究期间产生的卵和卵/雌性的总数量。收集卵并在18天的产卵期时间内进行计数。最后两栏列出了有活力的卵/雌性和产卵力的净降低(%)。使用实例中所述公式估算经VgR dsRNA处理的雌性相对于对照(暴露于水的雌性)的产卵力的净降低(NRF)。图3B显示了根据剂量的整体卵孵化率的百分比的箱线图。数据代表18天的采卵期;n=1-4个重复/处理/天;5-478个卵/重复,取决于天数和卵的可用性。图3C显示了以不同剂量的dsVgR处理后第6天,VgR相对表达的箱线图。将未经处理的甲虫用作正规化子。
图4A-4B显示了摄取各种VgR dsRNA片段后有关VgR基因抑制的数据。图4A显示了编码VgR的DNA序列(“CDS”)上的VgR片段和qRTPCR测定的扩增子(由虚线圆圈表示)的示意图。图4B显示了使用5′-qRTPCR测定用dsVgR片段和对照(ddH2O和dsGUS)处理后6天的相对VgR表达的箱线图。显示了4个四分位数、平均值(水平实线)、中值(水平虚线)和平均数的95%置信区间。用Mid-和3′-qRTPCR测定也得到类似的结果。将数据标准化为从未经处理的3龄幼虫获得的结果。
图5A-5D显示了有关使用基因抑制分析的VgR片段筛选的数据。图5A显示了在用于基因抑制分析的筛选中使用的VgR片段的示意图。图5B-5D显示了使用在三个实验中获得的结果针对所指示的VgR片段的代表性基因分析。在每个实验中,包括通过水、GUS和VgR片段1(SEQ ID NO.:3)的处理作为对照。将数据标准化为用水处理的甲虫。使用两种qRTPCR测定(5′-和Mid-qRTPCR测定)来避免VgR片段和PCR扩增子的重叠。
图6A-6B显示了摄取表达如图所示的VgR dsRNA构建体的转基因植物的甲虫中有关VgR基因抑制的数据。在每个图的底部指示了植物中的VgR表达。图6A显示了在大约V4生长阶段的植物被笼中的至少14只幼龄雌性甲虫侵染的数据。植物类型如图所示,其中“NTG”表示非转基因对照植物;“Frag1”表示表达包含VgR-Frag1(SEQ ID NO.:3)的沉默元件的转基因植物;“Frag2”表示表达包含VgR-Frag2(SEQ ID NO.:4)的沉默元件的转基因植物,以及“Frag3”表示表达包含VgR-Frag3(SEQ ID NO.:5)的沉默元件的转基因植物。饲喂8天后收集甲虫用于基因抑制分析。将数据标准化为来自摄取该NTG对照的甲虫的数据。图6B显示了从被笼中的超过6只幼龄雌性甲虫侵染的单个R1玉米植物获得的数据。饲喂后12天收集甲虫。每个片段和对照由用于饲喂的2个植物和用于基因抑制分析的超过12只昆虫表示。
图7显示了关于VgR T1甲虫成虫暴露生物测定的产卵力评估的数据。对于每个构建体,测试2-4个转殖项。每个笼子每天和/或以2-4天的间隔接受产卵盘,并随后进行加工。
图8显示了关于VgR T1幼虫暴露生物测定的产卵力评估的数据。针对每个转殖项,安排包含至少8-14对雄性和雌性甲虫的三个重复笼子。每个笼子每5天接收产卵盘,并加工卵。
图9A-9B显示了通过DV-BOULE-FRAG1(SEQ ID NO.:164)dsRNA处理的关于WCRW成虫不育化生物测定和基因抑制的数据。图9A显示了每只雌性产生的卵和受精卵的总数;平均卵孵化率与平均数标准误差;相对于水对照,每只雌性总产卵量的减少和雌性甲虫产卵力的净降低。图9B显示了BOULE dsRNA处理后甲虫中的基因表达。通过qRTPCR测定的相对表达描述于之前的实例中。该箱线图显示了四个四分位数、平均值(水平虚线)、中值(水平实线)和平均数的95%置信区间。
图10显示了关于来自幼虫暴露于表达BOULE FRAG1(SEQ ID NO.:164)dsRNA的T1转基因植物的甲虫计数的数据。该箱线图显示了四个四分位数、平均值(水平虚线)、中值(水平实线)和平均数的95%置信区间。使用体外转录(IVT)产物作为对照,在根部样品中测定针对每个转殖项的植物BOULE dsRNA片段的平均表达水平。
图11A-11C显示了关于WCRW幼虫暴露于导致成虫不育化的BOULE转基因T1植物的数据。图11A显示了幼虫暴露于转基因植物(表达DV-BOULE-FRAG1,SEQ ID NO.:164)对来自羽化的甲虫的每只雌性的平均产卵量和每只雌性产生的平均有活力的卵的影响。每条中的线代表平均数标准误差(±SEM),并且其后跟随相同的大写或小写字母的相同颜色条在统计学上不存在差异。图11B显示了幼虫暴露于转基因植物(表达DV-BOULE-FRAG1,SEQ IDNO.:164)对从羽化的甲虫获得的卵的孵化率的影响。该箱线图显示了四个四分位数、平均值(水平白线)和平均数的95%置信区间(垂直黑线)。平均值和相应的平均数标准误差(±SEM)在该箱线图的底部显示。对于每次处理,对5批卵进行卵孵化试验,并且每个处理评估总共至少1200-1285个卵的生活力。图11C表明了幼虫暴露于转基因植物(表达DV-BOULE-FRAG1,SEQ ID NO.:164)相对于NTG对照对羽化的甲虫成虫的产卵力的净降低的影响。该箱线图显示了四个四分位数、平均值(水平黑线)和平均数的95%置信区间(垂直黑线)。平均值和相应的平均数标准误差在该箱线图的底部显示。
图12显示了关于靶向以1ppm的DV-CUL3-FRAG1、DV-NCLB-FRAG1和DV-MAEL-FRAG1dsRNA(分别为SEQ ID No.:44、45和46)的dsRNA的3龄不育化生物测定的数据。示出了每只雌性产卵的平均总数、每只雌性产生的有活力卵的平均数量、平均卵孵化率;产卵量的平均降低以及产卵力的净降低(均相对于水对照)。对于每个参数,均展示了各自的平均数标准误差。
具体实施方式
将在下文中参考附图更全面地描述本文的公开内容,其中示出了一些但不是所有的可能的实施例。实际上,公开内容可以以许多不同的形式体现,并且不应被解释为局限于本文所阐述的实施例;而是提供这些实施例,使得本公开能满足适用的法律要求。
所披露的组合物和方法所涉及的领域中的技术人员将考虑到,本文所披露的许多修改和其他实施例具有前述描述和相关附图中呈现的教导的益处。因此,应当理解,这些公开内容不限于所披露的特定实施例,并且修改和其他实施例旨在包括在所附权利要求的范围内。尽管本文中采用了具体的术语,但这些术语仅在一般性和描述性意义上使用而并非用于限制目的。
还应当理解,本文使用的术语仅用于描述特定方面的目的,而不是限制性的。如在说明书和权利要求中所使用的,术语“包括”可以包括“由......组成”的方面。除非另有定义,本文使用的所有技术和科学术语具有与所披露的组合物和方法所属领域的普通技术人员通常理解的相同含义。在本说明书和下面的权利要求书中,将参考本文中所定义的若干术语。
如本文所使用的,单数形式“一种(a)”、“一种(an)”以及“该”包括复数个指示物,除非上下文中另外明确指明。因此,例如,提及“细胞”包括多个这样的细胞,并且提及“蛋白质”包括本领域技术人员已知的一种或多种蛋白质及其等效物等。本文使用的所有技术和科学术语具有与本发明所属领域的普通技术人员通常理解的相同的含义,除非另有明确说明。
I.概述
提供了采用一种或多种沉默元件的方法和组合物,所述沉默元件在被植物昆虫有害生物(例如鞘翅目、半翅目或鳞翅目植物有害生物,包括叶甲属、瘦跗叶甲属、条跳甲属、无网蚜属、小粉虱属、茶翅蝽属、稻绿蝽属或灰翅夜蛾属植物有害生物)摄取时,能够降低靶标序列在所述有害生物中的表达。在某些实施例中,所述靶标序列的表达降低能控制有害生物繁殖的能力,并且因此这些方法和组合物能够限制昆虫有害生物对植物的损害以及昆虫有害生物的扩散。本文披露了如SEQ ID NO.:1-53或107-254中所示的靶标多核苷酸、或其变体和片段、及其互补序列。提供了包含这些靶标多核苷酸的序列、互补序列、活性片段或变体的沉默元件,当所述沉默元件被有害生物摄取或与有害生物接触时降低所述靶标序列中的一者或多者的表达,并且从而通过例如昆虫不育化或通过应用昆虫不育技术(SIT;即,这些沉默元件与不育化活性相关)来防治有害生物群体。在一些实施例中,提供了包含编码沉默元件的多核苷酸的转基因植物,当所述转基因植物被有害生物摄取或与有害生物接触时降低所述靶标序列中的一者或多者的表达,并且从而通过例如昆虫不育化或通过应用SIT来防治有害生物群体。
在一个实施例中,方法涉及生产不育昆虫;以非常大的数量(天然昆虫数量的约10到100倍)将不育昆虫释放到环境中,以便与存在于该环境中的天然昆虫交配,其中与不育雄性交配的天然雌性产生未受精卵。在另外的实施例中,重复释放不育昆虫一次或多次,其中天然昆虫的数量减少,并且不育昆虫与天然昆虫的比例增加,使天然群体规模减小。
据了解,靶标有害生物RNA可能涉及雄性和/或雌性不育、精子数量减少、产卵量(产卵力)、性别比例、受精率(能育性)、性器官成熟、以及精子或卵生活力中的一种或多种。SIT已被用于通过释放一种或两种性别的不育昆虫来通过基于交配的方法来防治昆虫群体。在一个实施例中,SIT包括释放大量搜索野生雌性并与野生雌性交配的不育雄性昆虫,从而防止产生后代。已经报道了使用不同方案产生不育昆虫的SIT能防治蚊子群体,例如疟蚊(Anopheles)或伊蚊(Aedes)(例如,参见Whyard等人,(2015)Parasit.Vectors[寄生虫和载体],8:96;Benedict,M.Q.和A.S.Robinson(2003)Trends Parasitol.[寄生虫学趋势]19(8):349;以及Nolan等人,(2011)Genetica139∶33)。已经在巴西实地评估了SIT对埃及伊蚊(Aedes aegypti)群体的防治(Carvalho,D.O.(2015),PLoS.9(7):e0003864)。登革热、基孔肯雅热和现在的寨卡病毒都通过埃及伊蚊进行传播,埃及伊蚊是地球上分布最广泛的疾病携带载体之一。
在一个实施例中,方法涉及生产不育昆虫;以天然昆虫数量的约0.5、1、5、10、20、30、50、60、70、90、至100倍将不育昆虫释放到环境中,其中所述不育昆虫与存在于该环境中的天然昆虫交配,其中与不育雄性交配的天然雌性产生未受精卵。在另外的实施例中,重复释放不育昆虫一次或多次,其中天然昆虫的数量减少,并且不育昆虫与天然昆虫的比例增加,使天然群体规模减小。
在一个实施例中,提供了采用包含至少一个双链RNA区域的核糖核酸构建体的组合物和方法,所述双链RNA区域的至少一条链包含与以下互补的多核苷酸:(a)包含在靶标有害生物中表达的RNA转录物的序列的核苷酸序列,其中所述RNA转录物的下调导致所述靶标的不育增加;或其变体和片段,以及所述核苷酸序列的互补序列;(b)包含与所述核苷酸序列的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者(c)包含所述核苷酸序列的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列;其中所述多核苷酸编码对昆虫植物有害生物具有不育化活性的沉默元件。
在另外的实施例中,提供了采用包含至少一个双链RNA区域的核糖核酸构建体的组合物和方法,所述双链RNA区域的至少一条链包含与以下互补的多核苷酸:(a)包含在鞘翅目有害生物中表达的RNA转录物的序列的核苷酸序列,其中所述RNA转录物的下调导致所述靶标的不育增加;或其变体和片段,以及所述核苷酸序列的互补序列;(b)包含与所述核苷酸序列的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者(c)包含所述核苷酸序列的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列;其中所述多核苷酸编码对昆虫植物有害生物具有不育化活性的沉默元件。
在另一个实施例中,提供了采用包含至少一个双链RNA区域的核糖核酸构建体的组合物和方法,所述双链RNA区域的至少一条链包含与以下互补的多核苷酸:(a)包含SEQID NO.:1-53或107-254中任一项的核苷酸序列;或其变体和片段,及其互补序列;(b)包含与核苷酸SEQ ID NO:1-53或107-254中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者(c)包含SEQ ID NO:1-53或107-254中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列;其中所述多核苷酸编码对昆虫植物有害生物具有不育化活性的沉默元件。
如本文所使用的,“VgR蛋白”或“卵黄原蛋白受体蛋白”是指大的(180kDa-214kDa)膜结合蛋白家族,并且包括如下蛋白,例如具有SEQ ID NO.:106的序列的VgR蛋白,及其变体、同系物和突变体。据信,这些蛋白以高亲和力与卵黄原蛋白结合(Kd值约为30nM-180nM)并参与卵黄原蛋白的细胞摄取。VgR蛋白通常在卵巢组织中表达。如本文所使用的,“BOULE”是指编码具有高度保守的RRM(RNA识别基序)结构域的RNA结合蛋白和富含Asn、Tyr和Gln残基的24个氨基酸的至少一个DAZ(在无精子症中缺失)重复的基因家族。苍蝇中BOULE的缺失或突变通常严重损害精子形成。BOULE是进入减数分裂期以及在减数分裂G2期和M期之间过渡时的种系分化所必需的。BOULE通常在生殖系细胞中表达。
如本文所使用的,“VgR mRNA”或“卵黄原蛋白受体mRNA”是指当翻译时提供VgR蛋白,或其变体、同系物或突变体蛋白的信使RNA转录物。
如本文所使用的,“防治植物昆虫有害生物”或“能防治植物昆虫有害生物”意指使植物昆虫有害生物造成的损害受到限制的对有害生物的任何影响。防治植物昆虫有害生物包括但不限于杀死该有害生物、抑制该有害生物的发育、改变该有害生物的能育性或生长,以此种方式使该有害生物对植物的损害减小,或以某种方式减少所产生后代的数量、产生适应力较弱的有害生物(包括后代)、产生易受捕食者攻击的有害生物、产生更易受其他杀昆虫蛋白影响的有害生物、或阻止这些有害生物啃食植物。
降低有害生物中的靶标多核苷酸或由其编码的多肽的表达水平使得入侵有害生物被抑制、防治和/或杀死。在一个实施例中,减少有害生物的靶标序列的表达水平将使有害生物损害减少了至少约2%至至少约6%、至少约5%至约50%、至少约10%至约60%、至少约30%至约70%、至少约40%至约80%、或至少约50%至约90%或更高。因此,本文披露的方法可以用于防治有害生物,有害生物包括但不限于鞘翅目植物昆虫有害生物或叶甲属植物有害生物。
某些测量对植物昆虫有害生物的防治的测定法通常是本领域已知的,正如用以记录结节损伤评分的方法也是本领域已知的一样。参见,例如,Oleson等人(2005)J.Econ.Entomol.[经济昆虫学杂志]98∶1-8。其他测定方法在下面的实例中提供。
本文披露的是用于保护植物免受植物昆虫有害生物侵害或诱导植物中的对植物昆虫有害生物(例如鞘翅目植物有害生物或叶甲属植物有害生物或其他植物昆虫有害生物)抗性的组合物和方法。植物昆虫有害生物包括选自如下各目的昆虫:鞘翅目、双翅目、膜翅目、鳞翅目、食毛目、同翅目、半翅目、直翅目、缨翅目、革翅目、等翅目、虱目、蚤目、毛翅目等,特别是鳞翅目和鞘翅目。
本领域技术人员将认识到,并非所有组合物对所有有害生物都同样有效。披露的组合物(包括本文披露的沉默元件)显示出抗植物昆虫有害生物活性,该植物昆虫有害生物可以包括经济上重要的农艺、森林、温室、苗圃观赏植物、食物和纤维、公共和动物健康、家庭和商业结构、家用和储存产品有害生物。
如本文所使用的,“鞘翅目植物有害生物”用于指鞘翅目的任何成员。可以通过本文披露的方法和组合物进行靶向的其他植物昆虫有害生物包括但不限于墨西哥豆瓢虫(Epilachna varivestis)和马铃薯甲虫(Leptmotarsa decemlineata)。
如本文所使用的,术语“叶甲属植物有害生物”是指叶甲属的任何成员。因此,这些组合物和方法也可用于保护植物免受任何叶甲属植物有害生物的侵害,该叶甲属植物有害生物包括,例如柠条叶甲(Diabrotica adelpha);阿梅卡梅卡叶甲(Diabroticaamecameca),黄瓜条叶甲(Diabrotica balteata);北方玉米根虫(Diabrotica barberi);双环叶甲(Diabrotica biannularis);冠叶甲(Diabrotica cristata);十星叶甲(Diabrotica dcempunctata);异色叶甲(Diabrotica dissimilis);纽带叶甲(Diabroticalemniscata);小拟叶甲(Diabrotica limitata)(包括,例如,十五星小拟叶甲(Diabroticalimitata quindecimpuncata));长角叶甲(Diabrotica longicornis),钱币状叶甲(Diabrotica nummularis),中空叶甲(Diabrotica porracea);棘角叶甲(Diabroticascutellata);六斑叶甲(Diabrotica sexmaculata);南美叶甲(Diabrotica speciosa)(包括,例如,Diabrotica speciosa speciosa);胫叶甲(Diabrotica tibialis);黄瓜十一呈叶甲(Diabrotica undecimpunctata)(包括,例如,南方玉米根虫(黄瓜十一星叶甲(Diabrotica undecimpunctata)),Diabrotica undecimpunctata duodecimnotata;斑点黄瓜甲虫(Diabrotica undecimpunctata howardi,spotted cucumber beetle);西方斑点黄瓜甲虫(Diabrotica undecimpunctata undecimpunctata,western spotted cucumberbeetle));玉米根虫(Diabrotica virgifera)(包括,例如,玉米根萤叶甲(Diabroticavirgifera virgifera)(西方玉米根虫(western corn rootworm))和墨西哥玉米根虫(Diabrotica virgifera zeae,Mexican corn rootworm));绿叶甲(Diabroticaviridula),芜菁叶甲(Diabrotica wartensis),叶甲属物种JJG335,叶甲属物种JJG336;叶甲属物种JJG341,叶甲属物种JJG356;叶甲属物种JJG362;和叶甲属物种JJG365。
在某些实施例中,叶甲属植物有害生物包括玉米根萤叶甲、北方玉米根虫(D.barberi)、墨西哥玉米根虫、南美叶甲(D.speciosa)或斑点黄瓜甲虫(D.undecimpunctata howardi)。
鳞翅目的幼虫包括但不限于,夜蛾科秋粘虫(Spodoptera frugiperda JE Smith,fall armyworm)中的粘虫、夜蛾、尺蠖和棉铃虫(heliothines);甜菜夜蛾(S.exigua Hübner,beet armyworm);斜纹夜蛾(S.litrura Fabricius,tobacco cutworm,clustercaterpillar);蓓带夜蛾(Mamestra configurata Walker)(披肩粘虫(berthaarmyworm));甘蓝夜蛾(M.brassicae Linnaeus,cabbage moth);小地老虎(Agrotisipsilon Hufnagel,black cutworm);地老虎(A.orthogonia Morrison)(西方地老虎(western cutworm));A.subterranea Fabricius(颗粒状地老虎(granulate cutworm));棉叶波纹夜蛾(Alabama argillacea Hübner)(棉叶虫(cotton leaf worm));粉纹夜蛾(Trichoplusia ni Hübner,cabbage looper);大豆夜蛾(Pseudoplusia includensWalker)(大豆夜蛾(soybean looper));黎豆夜蛾(Anticarsia gemmatalis Hübner)(黎豆夜蛾(velvetbean caterpillar));苜蓿绿夜蛾(Hypena scabra Fabricius)(苜蓿绿夜蛾(green cloverworm));烟芽夜蛾(Heliothis virescens Fabricius)(烟草夜蛾(tobaccobudworm));一星黏虫(Pseudaletia unipuncta Haworth)(粘虫(armyworm));Athetismindara Barnes&Mcdunnough(粗皮地老虎(rough skinned cutworm));暗缘地老虎(Euxoamessoria Harris)(暗缘地老虎(darksided cutworm));棉斑实蛾(Earias insulanaBoisduval)(多刺螟蛉(spiny bollworm));翠纹钻夜蛾(E.vittella Fabricius)(斑点螟蛉(spotted bollworm));棉铃虫(Helicoverpa armigera Hübner)(美国螟蛉(Americanbollworm));棉铃虫(H.zea Boddie,corn earworm或cotton bollworm);Melanchra pictaHarris(斑马毛虫(zebra caterpillar));Egira(Xylomyges)curialis Grote(柑橘夜蛾(citrus cutworm));来自螟蛾科欧洲玉米螟(Ostrinia nubilalis Hübher,Europeancorn borer)的螟虫、鞘蛾、结网虫、锥形虫(coneworms)、和雕叶虫(skeletonizers);脐橙螟(Amyelois transitella Walker,naval orangeworm);地中海粉斑螟(Anagastakuehniella Zeller,Mediterranean flour moth);粉斑螟蛾(Cadra cautella Walker,almond moth);二化螟(Chilo suppressalis Walker,rice stem borer);斑禾草螟(C.partellus)(高粱螟(sorghum borer));米蛾(Corcyra cephalonica Stainton,ricemoth);玉米根草螟(Crambus caiiginosellus Clemens)(玉米根结网虫(corn rootwebworm));C.teterrellus Zincken(蓝草结网虫(bluegrass webworm));稻纵卷叶螟(Cnaphalocrocis medinalis Guenée,rice leaf roller);Desmia funeralis Hübher(葡萄甘薯麦蛾(grape leaffolder));绢野螟(Diaphania hyalinata Linnaeus)(瓜虫(melonworm));黄瓜绢野螟(D.llidis Stoll)(泡菜虫(pickleworm));巨座玉米螟(Diatraeagrandiosella Dyar)(西南玉米秆草螟(southwestern corn borer)),蔗螟(D.saccharalis Fabricius,surgarcane borer);墨西哥稻螟(Eoreuma loftini Dyar,Mexican rice borer);烟草螟(Ephestia elutella Hübner)(烟草(可可)蛾);大蜡螟(Galleria mellonella Linnaeus,greater wax moth);Herpetogramma licarsisalisWalker(草地螟(sod webworm));向日葵斑螟(Homoeosoma electellum Hulst,sunflowermoth);南美玉米苗斑螟(Elasmopalpus lignosellus Zeller)(小玉米茎蛀虫);小蜡蛾(Achroia grisella Fabricius,lesser wax moth);草地螟(Loxostege sticticalisLinnaeus,beet webworm);Orthaga thyrisalis Walker(茶树结网蛾(tea tree webmoth));豆荚螟(Maruca testulalis Geyer,bean pod borer);印度谷螟(Plodiainterpunctella Hübner,Indian meal moth);三化螟(Scirpophaga incertulas Walker,yellow stem borer);Udea rubigalis Guenée(芹菜卷叶螟(celery leaftier));和卷蛾科东部黑头长翅卷蛾(Tortricidae Acleris gloverana Walsingham)(西方黑头蚜虫)中的卷叶虫、蚜虫、种子虫和水果虫;A.variana Fernald(东部黑头蚜虫);果树黄卷蛾(Archips argyrospila Walker)(果树卷叶虫(fruit tree leaf roller));A.rosanaLinnaeus(欧洲卷叶虫(European leaf roller));以及其他黄卷蛾物种,棉褐带卷蛾(Adoxophyes orana Fischer von ,summer fruit tortrix moth);Cochylis hospes Walsingham(带状向日葵斑蛾(banded sunflower moth));Cydialatiferreana Walsingham(榛树虫(filbertworm));C.pomonella Linnaeus(苹果蠹蛾(coding moth));Platynota flavedana Clemens(杂色卷叶虫(variegatedleafroller));P.stultana Walsingham(杂食卷叶虫(omnivorous leafroller));葡萄花翅小卷蛾(Lobesia botrana Denis&Schiffermüller)(欧洲葡萄树蛾(European grapevine moth));苹果小卷蛾(Spilonota ocellana Denis&Schiffermüller,eyespotted budmoth);Endopiza viteana Clemens(葡萄浆果蛾(grape berry moth));女贞细卷蛾(Eupoecilia ambiguella Hübner)(藤蛾(vine moth));Bonagota salubricola Meyrick(巴西苹果卷叶虫(Brazilian apple leafroller));梨小食心虫(Grapholita molestaBusck)(东方水果蛾(oriental fruit moth));Suleima helianthana Riley(向日葵芽蛾(sunflower bud moth));带卷蛾属物种(Argyrotaenia spp.);卷叶蛾属物种(Choristoneura spp.)。
在鳞翅目中选择的其他农艺学有害生物包括但不限于,秋星尺蠖(Alsophilapometaria Harris,fall cankerworm);桃蚜蛾(Anarsia lineatella Zeller)(桃条麦蛾(peach twig borer));Anisota senatoria J.E.Smith(橙色条纹橡树虫(orange stripedoakworm));柞蚕(Antheraea pemyi Guérin-Méneville)(中国橡树柞蛾(Chinese OakTussah Moth));桑蚕(Bombyx mori Linnaeus,Silkworm);棉潜蛾(Bucculatrixthurberiella Busck,cotton leaf perforator);纹黄豆粉蝶(Colias eurythemeBoisduval)(苜蓿粉蝶(alfalfa caterpillar));Datana integerrima Grote&Robinson(胡桃毛虫(walnut caterpillar));西伯利亚松毛虫(Dendrolimus sibiricusTschetwerikov)(西伯利亚丝蛾(Siberian silk moth)),Ennomos subsignaria Hübner(榆树尺蠖(elm spanworm));Erannis tiliaria Harris(椴尺蠖(linden looper));褐尾蠹(Euproctis chrysorrhoea Linnaeus,browntail moth);Harrisina americana Guérin-Méneville(葡萄叶雕叶虫(grapeleaf skeletonizer));行列半白大蚕蛾(Hemileucaoliviae Cockrell)(山脉毛虫(range caterpillar));美国白蛾(Hyphantria cuneaDrury,fall webworm);番茄茎麦蛾(Keiferia lycopersicella Walsingham)(番茄蛲虫(tomato pinworm));铁杉尺蠖(Lambdina fiscellaria fiscellaria Hulst,Easternhemlock looper);L.fiscellaria lugubrosa Hulst(西方铁杉尺蠖(Western hemlocklooper));毒蛾(Leucoma salicis Linnaeus)(柳毒蛾(satin moth));舞毒蛾(Lymantriadispar Linnaeus,gypsy moth);番茄天蛾(Manduca quinquemaculata Haworth,fivespotted hawk moth,tomato hornworm);烟草天蛾(M.sexta Haworth)(番茄天蛾(tomatohornworm),烟草天蛾(tobacco hornworm));冬尺蠖蛾(Operophtera brumata Linnaeus,winter moth);春尺蠖(Paleacrita vemata Peck,spring cankerworm);美洲大芷凤蝶(Papilio cresphontes Cramer)(大燕尾柑桔凤蝶(giant swallowtail orange dog));Phryganidia californica Packard(加利福尼亚州橡木虫(California oakworm));桔潜蛾(Phyllocnistis citrella Stainton,citrus leafminer);Phyllonorycterblancardella Fabricius(斑点幕型潜叶虫(spotted tentiform leafminer));大菜粉蝶(Pieris brassicae Linnaeus,large white butterfly);菜青虫(P.rapae Linnaeus)(小菜粉蝶(small white butterfly));暗脉菜粉蝶(P.napi Linnaeus)(绿色纹理粉蝶(greenveined white butterfly));Platyptilia carduidactyla Riley(洋蓟羽蛾(artichokeplume moth));小菜蛾(Plutella xylostella Linnaeus,diamondback moth);红铃麦蛾(Pectinophora gossypiella Saunders)(棉红铃虫(pink bollworm));Pontia protodiceBoisduval&Leconte(南方菜青虫(Southern cabbageworm));Sabulodes aegrotata Guenée(杂食尺蠖(omnivorous looper));Schizura concinna J.E.Smith(红瘤毛虫(redhumped caterpillar));麦蛾(Sitotroga cerealella Olivier,Angoumois grain moth);松异带蛾(Thaumetopoea pityocampa Schiffermuller)(松树列队毛虫(pineprocessionary caterpillar));幕谷蛾(Tineola bisselliella Hummel)(织带衣蛾(webbing clothesmoth));番茄斑潜蝇(Tuta absoluta Meyrick)(番茄潜叶虫(tomatoleafminer));苹果巢蛾(Yponomeuta padella Linnaeus)(巢蛾(ermine moth));实夜蛾(Heliothis subflexa Guenée);天幕毛虫属物种(Malacosoma spp.)和古毒蛾属物种(Orgyia spp.)。
目的是鞘翅目的幼虫和成虫,其包括来自长角象科(Anthribidae)、豆象科(Bruchidae)和象甲科(Curculionidae)的象鼻虫(包括但不限于:墨西哥棉铃象(Anthonomus grandis Boheman)(棉铃象甲(boll weevil));稻水象甲(Lissorhoptrusoryzophilus Kuschel)(稻水象虫(rice water weevil));谷象鼻虫(Sitophilusgranarius Linnaeus)(谷象(granary weevil));稻象虫(S.oryzae Linnaeus,riceweevil);三叶草叶象(Hypera punctata Fabricius)(车轴草叶象虫(clover leafweevil));密点细枝象(Cronrocopturus adspersus LeConte)(向日葵茎象鼻虫(sunflower stem weevil));黄褐小爪象(Smicronyx fulvus LeConte)(红色葵花籽象鼻虫(red sunflower seed weevil));S.sordidus LeConte(灰色葵花籽象鼻虫(graysunflower seed weevil));Sphenophorus maidis Chittenden(玉米象虫(maizebillbug));叶甲科中的跳甲、黄瓜叶甲、根虫、叶甲虫、马铃薯甲虫和潜叶虫(包括但不限于:科罗拉多马铃薯甲虫(Leptinotarsa decemlineata Say,Colorado potato beetle);玉米根萤叶甲(Diabrotica virgifera virgifera LeConte)(西方玉米根虫(western comrootworm));北方玉米根虫(D.barberi Smith and Lawrence)(北方玉米根虫(northerncorn rootworm)));斑点黄瓜甲虫(D.undecimpunctata howardi Barber)(南方玉米根虫(southern corn rootworm));玉米跳甲(Chaetocnema pulicaria Melsheimer,corn fleabeetle);十字花科条跳甲(Phyllotreta cruciferae Goeze,Crueifer flea beetle);黄曲条跳甲(Phyllotreta striolata,stripped flea beetle);Colaspis brunneaFabricius(葡萄肖叶甲(grape colaspis));橙足负泥虫(Oulema melanopus Linnaeus)(谷物叶甲(cereal leaf beetle));Zygogramma exclamationis Fabricius(向日葵叶甲(sunflower beetle));来自瓢虫科的甲虫(包括但不限于:墨西哥豆瓢虫(Epilachnavarivestis Mulsant,Mexican bean beetle));来自金龟子科的金龟子和其他甲虫(包括但不限于:日本金龟子(Popillia japonica Newman)(日本金龟(Japanese beetle));Cyclocephala borealis Arrow(北方独角仙(northern masked chafer),蛴螬(whitegmb));C.immaculata Olivier(南方独角仙(southern masked chafer),蛴螬(whitegrub));欧洲切根鳃金龟(Rhizotrogus majalis Razoumowsky)(欧洲金龟子(Europeanchafer));具毛鳃角金龟(Phyllophaga crinita Burmeister)(蛴螬(white grub));Ligyrus gibbosus De Geer(胡萝卜甲虫(carrot beetle));来自皮蠹科家族的红缘皮蠹;来自叩头虫科(Elateridae)、伪金针虫属物种(Eleodes spp.)、梳爪叩头虫属物种(Melanotus spp.)的金针虫;宽胸金针虫属物种(Conoderus spp.);叩甲属物种(Limoniusspp.);缺隆叩甲属物种(Agriotes spp.);特尼塞拉属物种(Ctenicera spp.);埃俄罗斯属物种(Aeolus spp.);来自小蠹科(Scolytidae)的树皮甲虫和来自拟步甲科(Tenebrionidae)的甲虫。
目的是双翅目的成虫和未成熟的虫,包括潜叶虫玉米斑潜蝇(Agromyzaparvicornis Loew)(玉米斑潜蝇(corn blotch leafminer));瘿蚊(包括但不限于:高梁瘿蚊(Contarinia sorghicola Coquillett,sorghum midge);小麦黑森瘿蚊(Mayetioladestructor Say,Hessian fly);麦红吸浆虫(Sitodiplosis mosellana Géhin,wheatmidge);向日葵籽瘿蚊(Neolasioptera murtfeldtiana Felt,sunflower seed midge);果蝇(实蝇科(Tephritidae)),瑞典麦秆蝇(Oscinella frit Linnaeus)(果蝇);蛆(包括但不限于:灰地种蝇(Delia platura Meigen)(玉米种蛆(Oscinellafrit));麦地种蝇(D.coarctata Fallen)(麦种蝇(wheat bulb fly))和其他地种蝇属物种,美洲麦秆蝇(Meromyza americana Fitch)(美洲麦秆蝇(wheat stem maggot));家蝇(Muscadomestica Linnaeus,house flies);黄腹厕蝇(Fannia canicularis Linnaeus),F.femoralis Stein(小家蝇(lesser house flies));厩螫蝇(Stomoxys calcitransLinnaeus,stableflies);秋家蝇(face flies)、角蝇(hornflies)、丽蝇(blowflies)、金蝇属物种(Chrysomya spp.);伏蝇属物种(Phormia spp.)和其他蝇状的蝇类有害生物,虻属物种(Tabanus spp.);肤蝇(bot flies)胃蝇属物种(Gastrophilus spp.);狂蝇属物种(Oestrus spp.);纹皮蝇(cattle grubs)皮蝇属物种(Hypoderma spp.);鹿虻(deerflies)斑虻属物种(Chrysops spp.);绵羊虱蝇(Melophagus ovinus Linnaeus)(羊蜱蝇(keds))和其他短角亚目(Brachycera),蚊子伊蚊属物种(Aedes spp.);疟蚊属物种(Anophelesspp.);家蚊属物种(Culex spp.);黑蝇(black flies)原蚋属物种(Prosimulium spp.);蚋属物种(Simulium spp.);蠓、沙蝇、眼菌蚊和长角亚目。
作为目的昆虫包括了半翅目和同翅目的成虫和若虫,如但不限于:来自球蚜科(Adelgidae)的球蚜、来自盲蝽科(Miridae)的盲蝽、来自蝉科(Cicadidae)的蝉、叶蝉、小绿叶蝉属(Empoasca)物种;来自叶蝉科的,来自菱蜡蝉科(Cixiidae)、青翅飞虱科(Flatidae)、蜡蝉总科(Fulgoroidea)、瓢蜡蝉科(Issidae)和(Delphacidae)的飞虱,来自角蝉科(Membracidae)的角蝉,来自木虱科(Psyllidae)的木虱,来自粉虱科(Aleyrodidae)的粉虱,来自蚜科(Aphididae)的蚜虫,来自根瘤蚜科(Phylloxeridae)的葡萄根瘤蚜,来自粉蚧科(Pseudococcidae)的粉蚧,来自链介壳虫科(Asterolecanidae)、蚧科(Coccidae)、粉蚧科(Dactylopiidae)、盾蚧科(Diaspididae)、绒蚧科(Eriococcidae)、旌介壳虫科(Ortheziidae)、刺葵介壳虫科(Phoenicococcidae)和绵蚧科(Margarodidae)的介壳虫,来自网蝽科的网蝽,来自蝽科(Pentatomidae)的椿象,长蝽(cinch bug),土长蝽属物种;和来自长蝽科的其他长蝽,来自沫蝉科的沫蝉,来自缘蝽科的瓜椿象和来自红蝽科的秋恙螨和污棉虫。
来自同翅目的农艺学上重要成员进一步包括但不限于:Acyrthisiphon pisumHarris(豌豆蚜(pea aphid));黑豆蚜(Aphis craccivora Koch)(豇豆蚜(cowpeaaphid));甜菜蚜(A.fabae Scopoli)(黑豆蚜(black bean aphid));棉蚜(A.gossypiiGlover)(棉蚜虫(cotton aphid,melon aphid));槐蚜(A.maidiradicis Forbes)(玉米根蚜(corn root aphid)));苹果蚜(A.pomi De Geer,apple aphid);绣线菊蚜(A.spiraecola Patch,spirea aphid);茄沟无网蚜(Aulacorthum solani Kaltenbach)(毛地黄蚜虫(foxglove aphid));草莓钉蚜(Chaetosiphon fragaefolii Cockerell)(草莓蚜(strawberry aphid));麦双尾蚜(Diuraphis noxia Kurdjumov/Mordvilko,Russianwheat aphid);车前圆尾蚜(Dysaphis plantaginea Paaserini)(红苹果蚜(rosy appleaphid));苹果绵蚜(Eriosoma lanigerum Hausmann,woolly apple aphid);甘蓝蚜(Brevicoryne brassicae Linnaeus,cabbage aphid);大尾蚜(Hyalopterus pruniGeoffroy)(桃大尾蚜(mealy plum aphid));萝卜蚜(Lipaphis erysimi Kaltenbach,turnip aphid);麦无网蚜(Metopolophium dirrhodum Walker)(谷物蚜虫(cerealaphid));马铃薯蚜(Macrosiphum euphorbiae Thomas,potato aphid);桃蚜(Myzuspersicae Sulzer)(桃-马铃薯蚜虫,绿桃蚜虫);莴苣蚜(Nasonovia ribisnigri Mosley,lettuce aphid);瘿绵蚜属物种(Pemphigus spp.)(根蚜(root aphid)和瘿蚜(gallaphid));玉米蚜(Rhopalosiphum maidis Fitch,corn leaf aphid);稻麦蚜(R.padiLinnaeus)(禾谷缢管蚜(bird cherry-oat aphid));麦二叉蚜(Schizaphis graminumRondani,greenbug);甘蔗黄蚜(Sipha flava Forbes,yellow sugarcane aphid);麦长管蚜(Sitobion avenae Fabricius,English grain aphid);苜蓿斑蚜(Therioaphismaculata Buckton,spotted alfalfa aphid);茶二叉蚜(Toxoptera aurantii Boyer deFonscolombe,black citrus aphid)和桔二叉蚜(T.citricida Kirkaldy,brown citrusaphid);球蚜属物种(Adelges spp.)(球蚜(adelgid));Phylosera devastatrix Pergande(山核桃木虱(pecan phylloxera));烟粉虱(Bemisia tabaci Gennadius)(烟粉虱(tobacco whitefly),甘薯粉虱(sweetpotato whitefly));银叶粉虱(B.argentifoliiBellows&Perring,silverleaf whitefly);柑桔粉虱(Dialeurodes citri Ashmead,citrus whitefly);Trialeurodes abutiloneus(带状有翼粉虱(bandedwingedwhitefly))和温室粉虱(T.vaporariorum Westwood,greenhouse whitefly);马铃薯小绿叶蝉(Empoasca fabae Harris)(马铃薯叶蝉(potato leafhopper));灰飞虱(LaodelphaXstriatellus Fallen)(小褐飞虱(smaller brown planthopper));Macrolestesquadrilineatus Forbes(紫菀叶蝉(aster leafhopper));黑尾叶蝉(Nephotettixcinticeps Uhler)(青大叶蝉(green leafhopper));二条斑黑尾叶蝉(N.nigropictus )(稻叶蝉(rice leafhopper));稻褐飞虱(Nilaparvata lugens )(褐飞虱(brownplanthopper));玉米蜡蝉(Peregrinus maidis Ashmead)(玉米飞虱(cornplanthopper));白背飞虱(Sogatella furcifera Horvath,white-backed planthopper);稻飞虱(Sogatodes orizicola Muir,rice delphacid);Typhlocyba pomaria McAtee(白苹果叶蝉(white apple leafhopper));葡萄斑叶蝉属物种(Erythroneoura spp.)(葡萄叶蝉(grape leafhopper));十七年蝉(Magicicada septendecim Linnaeus)(晚秀蝉(periodical cicada));吹绵蚧(Icerya purchaseasi Maskell,cottony cushionscale);梨圆蚧(Quadraspidiotus perniciosus Comstock,San Jose scale);桔粉蚧(Planococcus citri Risso,citrus mealybug);粉蚧属物种(Pseudococcus spp.)(其他粉蚧复合体);梨木虱(Cacopsylla pyricola Foerster,pear psylla);Trioza diospyriAshmead(柿木虱(persimmon psylla))。
来自半翅目的农艺学上重要的物种包括但不限于:喜绿蝽(Acrosternum hilareSay,green stink bug);南瓜缘蝽(Anasa tristis De Geer,squash bug);Blissusleucopterus leucopterus Say(麦长蝽(chinch bug));Corythuca gossypii Fabricius(棉网蝽(cotton lace bug));Cyrtopeltis modesta Distant(番茄虫(tomato bug));Dysdercus suturellus Herrich-(污棉虫(cotton stainer));褐臭蝽(Euschistus servus Say,brown stink bug);E.variolarius Palisot de Beauvois(单斑椿象(one-spotted stink bug));红腺长蝽属物种(Graptostethus spp.)(长蝽的复合体);Leptoglossus corculus Say(叶足松树长蝽(leaf-footed pine seed bug));美洲牧草盲蝽(Lygus lineolaris Palisot de Beauvois)(牧草盲蝽(tarnished plant bug));豆荚盲蝽(L.Hesperus Knight)(西方牧草盲蝽(Western tarnished plant bug));牧草盲蝽(L.pratensis Linnaeus)(常见的草地虫);长毛草盲蝽(L.rugulipennis Poppius)(欧洲牧草盲蝽(European tarnished plant bug));Lygocoris pabulinus Linnaeus(苹绿盲蝽(common green capsid));南方绿椿象(Nezara viridula Linnaeus)(南方喜绿蝽(southern green stink bug));美洲稻蝽(Oebalus pugnax Fabricius)(稻椿象(ricestink bug));马利筋长蝽(Oncopeltus fasciatus Dallas)(大马利筋长蝽(largemilkweed bug));棉跳盲蝽(Pseudatomoscelis seriatus Reuter,cotton fleahopper)。
此外,实施例可能对半翅目有效,如Calocoris norvegicus Gmelin(草莓虫(strawberry bug));Orthops campestris Linnaeus;Plesiocoris rugicollis Fallen(苹盲蝽(apple capsid));Cyrtopeltis modesms Distant(番茄虫(tomato bug));黑斑烟盲铸(Cyrtopeltis notatus Distant)(吸蝇(suckfly));Spanagonicus albofasciatusReuter(水印跳盲蝽(whitemarked fleahopper));Diaphnocoris chlorionis Say(皂荚植食蝽(honeylocust plant bug));Labopidicola allii Knight(洋葱植食蝽(onion plantbug));棉跳盲蝽(Pseudatomoscelis seriatus Reuter,cotton fleahopper);Adelphocoris rapidus Say(快速植食蝽(rapid plant bug));Poecilocapsus lineatusFabricius(四线植食蝽(four-lined plant bug));小长蝽(Nysius ericae Schilling)(谷长蝽(false chinch bug));Nysius raphanus Howard(谷长蝽(false chinch bug));南方绿椿象(Nezara viridula Linnaeus)(南方喜绿蝽(southern green stink bug));扁盾蝽属物种(Eurygaster spp.);缘蝽属物种(Coreidae spp.);红蝽属物种(Pyrrhocoridae spp.);谷蛾属物种(Tinidae spp.);负子蝽科物种(Blostomatidaespp.);猎蝽属物种(Reduviidae spp.)和臭虫属物种(Cimicidae spp.)。
还包括蜱螨目(螨)的成虫和幼虫,如Aceria tosichella Keifer(小麦卷曲螨(wheat curl mite));麦岩螨(Petrobia latens Müller)(棕色小麦螨(brown wheatmite));叶螨科中的蜘蛛螨(spider mite)和红螨(red mite),苹果全爪螨(Panonychusulmi Koch)(欧洲红螨(European red mite));二点叶螨(Tetranychus urticae Koch,twospotted spider mite);迈叶螨(T.medanieli McGregor,McDaniel mite);朱砂虫螨(T.cinnabarinus Boisduval)(胭脂红蜘蛛螨(carmine spider mite));T.turkestaniUgarov&Nikolski(草莓蜘蛛螨(strawberry spider mite));细须螨科中的平螨,短须螨(Brevipalpus lewisi McGregor)(柑橘平螨(citrus flat mite));瘿螨科中的锈螨和蚜螨,和其他叶子喂养的螨虫和在人类和动物健康方面有重要影响的螨虫,蠕形螨科中的毛囊螨,食甜螨科中的谷物螨,蜱亚目中的蜱。肩突硬蜱(Ixodes scapularis Say)(鹿蜱(deer tick));全环硬蜱(I.holocyclus Neumann)(澳大利亚麻痹蜱(Australianparalysis tick));变异革蜱(Dermacentor variabilis Say)(美洲犬蜱(American dogtick));美洲钝眼蜱(Amblyomma americanum Linnaeus)(孤星蜱(lone star tick))和痒螨科、蒲螨科和疥螨科中的疥螨和痒螨。
目的是缨尾目的昆虫有害生物,如衣鱼(Lepisma saccharina Linnaeus)(蠹虫(silverfish));家衣鱼(Thermobia domestica Packard,firebrat)。
目的昆虫有害生物包括椿象和其他相关昆虫的超家族,包括但不限于属于以下各科的物种:蝽科(稻绿蝽、茶翅蝽(Halyomorpha halys)、Piezodorus guildini、褐臭蝽、拟绿蝽、英雄美洲蝽(Euschistus heros)、美洲蝽(Euschistus tristigmus)、拟绿蝽、褐蝽(Dichelops melacanthus)、和蓓蝽(Bagrada hilaris)(蓓蝽(Bagrada Bug))),龟蝽科(Plataspidae)(筛豆龟蝽(Megacopta cribraria)-豆平腹蝽蟓(Bean plataspid))和土蝽科(Scaptocoris castanea-Root stink bug),以及鳞翅目物种包括但不限于:小菜蛾,例如,玉米穗虫;大豆尺蠖,例如,大豆尺夜蛾(Pseudoplusia includens Walker),以及绒毛豆毛虫,例如,大豆夜蛾(Anticarsia gemmatalis Hübner)。
II.靶标序列
如本文所使用的,“靶标序列”或“靶标多核苷酸”包括有害生物中的希望降低其表达水平的任何序列。在某些实施例中,降低所述靶标序列在有害生物中的表达水平能防治该有害生物。例如,该靶标序列可以是生长和发育所必需的。靶标序列的非限制性实例包括SEQ ID NO.:1-53或107-254中所示的多核苷酸,或其变体和片段、及其互补序列。如本文别处所示例的,降低这些靶标序列中的一者或多者在鞘翅目植物有害生物或叶甲属植物有害生物中的表达水平能防治有害生物。
III.沉默元件
“沉默元件”意指当被植物昆虫有害生物接触或摄取时能够降低或消除靶标多核苷酸或其编码的多肽的水平或表达的多核苷酸。因此,应当理解的是,本文所用的“沉默元件”包含如下多核苷酸:例如RNA构建体、双链RNA(dsRNA)、发夹RNA以及正义和/或反义RNA。在一个实施例中,所用的沉默元件可通过影响靶标RNA转录物的水平,或者可替代地,通过影响翻译并由此影响所编码的多肽的水平来降低或消除靶标序列的表达水平。本文别处披露了测定功能性沉默元件的方法,该沉默元件能够降低或消除目的序列的水平。披露的方法中所采用的单个多核苷酸可包含针对相同或不同靶标多核苷酸的一个或多个沉默元件。该沉默元件可在体内(即,在宿主细胞诸如植物或微生物中)或体外生成。
在某些实施例中,沉默元件可以包含如下嵌合构建分子,该嵌合构建分子包含两个或更多个本披露的序列或其部分。例如,该嵌合构建体可为如本文所披露的发夹或dsRNA。嵌合体可包含两个或更多个所披露的序列或其部分。在一个实施例中,设想出嵌合体具有本文所示的两个互补序列或其部分,这两个互补序列之间具有一定程度的错配,使得这两个序列彼此并非完全互补。在单一沉默元件中提供至少两个不同序列可以允许使用一个沉默元件和/或例如一个表达盒靶向多个基因。靶向多个基因可以允许减缓或降低有害生物抗性的可能性。此外,在一个表达的分子中提供多重靶向能力可减轻经转化的植物或植物产物的表达负担,或提供能够用一次施用靶向多个宿主的局部处理。
在某些实施例中,虽然沉默元件能防治有害生物,但优选地沉默元件对正常植物或植物部分无作用。
如下文进一步详细讨论的,沉默元件可以包括但不限于,有义抑制元件、反义抑制元件、双链RNA、siRNA、amiRNA、miRNA或发夹抑制元件。在实施例中,沉默元件可以包含如下嵌合体,其中在相同的RNA分子中发现两个或更多个本披露的序列、或者活性片段或变体、或者其互补序列。在各种实施例中,本披露的序列、或者活性片段或变体、或者其互补序列可以作为多于一个拷贝存在于DNA构建体、沉默元件、DNA分子或RNA分子中。在发夹或dsRNA分子中,有义或反义序列在分子中的位置(例如,其中序列首先进行转录或位于该RNA分子的具体末端上)对所披露的序列来说并不是限制性的,并且dsRNA不受本文公开内容中这种序列的具体位置的限制。可用于降低这些靶标序列表达的沉默元件的非限制性实例,包括该有义或反义序列的片段或变体,或者可替代地,由该有义或反义序列和SEQ ID NO.:1-53或107-254中所示序列、或者其变体和片段、及其互补序列组成。该沉默元件可以进一步包含有利地影响转录和/或所得转录物的稳定性的另外的序列。例如,该沉默元件可以在3′端包含至少一个胸腺嘧啶残基。这有助于稳定。因此,该沉默元件在3′端可具有至少1、2、3、4、5、6、7、8、9、10或更多个胸腺嘧啶残基。如下文进一步详细讨论的,增强子抑制元件也可以与本文所披露的沉默元件结合使用。
通过“减少”或“降低”多核苷酸或由其编码的多肽的表达水平,旨在表示,该靶标序列的多核苷酸或多肽水平在统计学上低于适当的对照有害生物中相同靶标序列的多核苷酸水平或多肽水平,该对照有害生物没有暴露于(即没有摄取或接触)沉默元件。在具体实施例中,本文披露的方法和/或组合物降低植物昆虫有害生物中靶标序列的多核苷酸水平和/或多肽水平,得到多核苷酸水平和/或多肽水平是适当的对照有害生物中相同靶标序列的多核苷酸水平或由其编码的多肽的水平的小于95%、小于90%、小于80%、小于70%、小于60%、小于50%、小于40%、小于30%、小于20%、小于10%或小于5%。在一些实施例中,沉默元件与靶标多核苷酸具有大量的序列同一性,通常大于约65%序列同一性,大于约85%序列同一性,约90%、91%、92%、93%、94%、95%、96%、97%、98%或99%序列同一性。此外,沉默元件可以与靶标多核苷酸的一部分互补。通常,可以使用SEQ ID NO.:1-53或107-254中任一项所示的序列、或者其变体和片段、及其互补序列的至少15、16、17、18、19、20、22、25、50、100、200、300、400、450个或更多个连续核苷酸的序列。本文别处讨论了测定RNA转录物水平、经编码的多肽水平、或者该多核苷酸或多肽活性的方法。
i.有义抑制元件
如本文所使用的,“有义抑制元件”包括如下多核苷酸,该多核苷酸被设计用于表达对应于“有义”方向的靶标信使RNA的至少一部分的RNA分子。包含有义抑制元件的RNA分子的表达能降低或消除靶标多核苷酸或由其编码的多肽的水平。包含有义抑制元件的多核苷酸可以对应于靶标多核苷酸序列的全部或一部分、靶标多核苷酸的5′和/或3′非翻译区的全部或一部分、靶标多核苷酸的编码序列的全部或一部分、或靶标多核苷酸的编码序列和非翻译区二者的全部或一部分。
通常,有义抑制元件与靶标多核苷酸具有大量的序列同一性,通常大于约65%序列同一性,大于约85%序列同一性,约90%、91%、92%、93%、94%、95%、96%、97%、98%或99%序列同一性。参见美国专利号5,283,184和5,034,323;以上专利通过引用并入本文。有义抑制元件可以是任何长度的,只要其允许抑制靶标序列即可。该有义抑制元件可以是例如,SEQ ID NO.:1-53或107-254中任一个所示的靶标多核苷酸、或其变体和片段、以及其互补序列的15、16、17、18、19、20、22、25、30、50、100、150、200、250、300、350、400、450、500、600、700、900、1000、1100、1200、1300个核苷酸或更长。在其他实施例中,该有义抑制元件可以是例如,SEQ ID NO.:1-53或107-254中任一个所示的靶标多核苷酸、或其变体和片段、以及其互补序列的约15-25、19-35、19-50、25-100、100-150、150-200、200-250、250-300、300-350、350-400、450-500、500-550、550-600、600-650、650-700、700-750、750-800、800-850、850-900、900-950、950-1000、1000-1050、1050-1100、1100-1200、1200-1300、1300-1400、1400-1500、1500-1600、1600-1700、1700-1800个核苷酸或更长。
ii.反义抑制元件
如本文所使用的,“反义抑制元件”包括被设计用于表达与靶标信使RNA的全部或部分互补的RNA分子的多核苷酸。反义RNA抑制元件的表达能降低或消除靶标多核苷酸的水平。用于反义抑制中的多核苷酸可以对应于编码靶标多核苷酸的序列的互补序列的全部或部分、靶标多核苷酸的5′和/或3′非翻译区的互补序列的全部或部分、靶标多核苷酸的编码序列的互补序列的全部或部分、或靶标多核苷酸的编码序列和非翻译区两者的互补序列的全部或部分。此外,该反义抑制元件可以与靶标多核苷酸完全互补(即,与靶标序列的互补序列100%相同)或部分互补(即,与靶标序列的互补序列低于100%相同)。在某些实施例中,该反义抑制元件包含与靶标多核苷酸的至少85%、90%、91%、92%、93%、94%、95%、96%、97%、98%或99%序列互补性。反义抑制可用于抑制同一植物中多种蛋白质的表达。参见例如美国专利号5,942,657。此外,该反义抑制元件可以与靶标多核苷酸的一部分互补。通常,可以使用SEQ ID NO.:1-53或107-254中任一项所示的序列、或者其变体和片段、及其互补序列的至少15、16、17、18、19、20、22、25、50、100、200、300、400、450个或更多个核苷酸的序列。用于使用反义抑制来抑制植物中内源基因表达的方法描述于以下文献中:例如,Liu等人(2002)Plant Physiol.[植物生理学]129:1732-1743和美国专利号5,942,657(其通过引用并入本文)。
iii.双链RNA抑制元件
“双链RNA沉默元件”或“dsRNA”包含至少一种能够在被植物昆虫有害生物摄取之前或之后形成dsRNA的转录物。因此,“dsRNA沉默元件”包括dsRNA、能够形成dsRNA的转录物或多核糖核苷酸或超过一种能够形成dsRNA的转录物或多核糖核苷酸。“双链RNA”或“dsRNA”是指由单个自互补的RNA分子形成的多核糖核苷酸结构或由至少两个不同的RNA链的表达形成的多核糖核苷酸结构。所披露的方法和组合物中使用的一种或多种dsRNA分子介导靶标序列表达的降低,例如通过以序列特异性方式介导RNA干扰“RNAi”或基因沉默。在各种实施例中,dsRNA能够降低或消除植物昆虫有害生物中靶标多核苷酸或由其编码的多肽的水平或表达。
dsRNA可以通过影响靶标RNA转录物的水平、通过影响翻译并从而影响经编码的多肽的水平、或通过影响转录前水平上的表达(即,经由染色质结构的调节、甲基化状态等来改变基因表达)来降低或消除靶标序列的表达水平。例如,参见Verdel等人,(2004)Science[科学]303:672-676;Pal-Bhadra等人,(2004)Science[科学]303:669-672;Allshire,(2002)Science[科学]297:1818-1819;Volpe等人,(2002)Science[科学]297:1833-1837;Jenuwein,(2002)Science[科学]297:2215-2218;以及Hall等人,(2002)Science[科学]297:2232-2237。本文别处披露了测定功能性dsRNA的方法,该dsRNA能够降低或消除目的序列的水平。因此,如本文所使用的,术语“dsRNA”意在包括用于描述能够介导RNA干扰或基因沉默的核酸分子的其他术语,包括例如短干扰RNA(siRNA)、双链RNA(dsRNA)、微RNA(miRNA)、发夹RNA、短发夹RNA(shRNA)、转录后基因沉默RNA(ptgsRNA)等。
在某些实施例中,dsRNA的双链体或双链区域的至少一条链与靶标多核苷酸共享足够的序列同一性或序列互补性,以允许dsRNA降低靶标序列的表达水平。在一些实施例中,dsRNA与靶标多核苷酸具有大量的序列同一性,通常大于约65%序列同一性,大于约85%序列同一性,约90%、91%、92%、93%、94%、95%、96%、97%、98%或99%序列同一性。此外,dsRNA元件可以与靶标多核苷酸的一部分互补。通常,可以使用SEQ ID NO.:1-53或107-254中任一项所示的序列、或者其变体和片段、及其互补序列的至少15、16、17、18、19、20、22、25、50、100、200、300、400、450个或更多个核苷酸的序列。如本文所使用的,与靶标多核苷酸互补的链是“反义链”,并且与靶标多核苷酸同源的链是“有义链”。
在另一个实施例中,dsRNA包含发夹RNA。发夹RNA包含能够折回到自身上以形成双链结构的RNA分子。多种结构都可以用作发夹元件。在某些实施例中,该dsRNA抑制元件包含如下发夹元件,该发夹元件依次包含第一区段、第二区段和第三区段,其中该第一和第三区段共享足够的互补性,以允许经转录的RNA形成双链茎环结构。
该发夹的“第二区段”包含“环”或“环区”。这些术语在本文中作为同义词使用,并且广义地被解释为包含任何如下核苷酸序列,该核苷酸序列赋予足够灵活性以允许多核苷酸的互补区域(即,形成发夹茎的区段1和3)之间发生自配对。例如,在一些实施例中,该环区域可以基本上是单链的并且作为发夹茎环的自互补区域之间的间隔区。在一些实施例中,该环区可以包含随机或无义核苷酸序列,并且因此不与靶标多核苷酸共享序列同一性。在其他实施例中,该环区包含与靶标多核苷酸共享同一性的有义或反义RNA序列或者其片段。参见,例如,国际专利公开号WO 02/00904。在某些实施例中,该环序列可以包括内含子序列、源自内含子序列的序列、与内含子序列同源的序列、或经修饰的内含子序列。该内含子序列可以是与衍生出区段1和3相同或不同物种中发现的序列。在某些实施例中,可以将环区优化为尽可能短,同时仍然提供足够的分子内灵活性以允许形成碱基配对的茎区。因此,该环序列通常小于1000、900、800、700、600、500、400、300、200、100、50、25、20、19、18、17、16、15、10个核苷酸或更少。
发夹RNA分子的“第一”和“第三”区段包含发夹结构的碱基配对的茎。第一和第三区段是彼此的反向重复序列,并且共享足够的互补性以允许形成碱基配对的茎区。在某些实施例中,第一和第三区段彼此完全互补。可替代地,第一和第三区段可以彼此部分互补,只要它们能够彼此杂交形成碱基配对的茎区就可以。第一和第三区段之间的互补性的量可以计算为整个区段的百分比。因此,发夹RNA的第一和第三区段通常共享至少50%、60%、70%、80%、85%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%、至多且包括100%的互补性。
第一和第三区段至少约为1000、500、475、450、425、400、375、350、325、300、250、225、200、175、150、125、100、75、60、50、40、30、25、22、20、19、18、17、16、15或10个核苷酸的长度。在某些实施例中,该第一和/或第三区段的长度为约10-100个核苷酸、约10至约75个核苷酸、约10至约50个核苷酸、约10至约40个核苷酸、约10至约35个核苷酸、约10至约30个核苷酸、约10至约25个核苷酸、约10至约19个核苷酸、约10至约20个核苷酸、约19至约50个核苷酸、约50个核苷酸至约100个核苷酸、约100个核苷酸至约150个核苷酸、约100个核苷酸至约300个核苷酸、约150个核苷酸至约200个核苷酸、约200个核苷酸至约250个核苷酸、约250个核苷酸至约300个核苷酸、约300个核苷酸至约350个核苷酸、约350个核苷酸至约400个核苷酸、约400个核苷酸至约500个核苷酸、约600nt、约700nt、约800nt、约900nt、约1000nt、约1100nt、约1200nt、1300nt、1400nt、1500nt、1600nt、1700nt、1800nt、1900nt、2000nt或更长。在其他实施例中,第一和/或第三区段的长度包含至少10-19个核苷酸、10-20个核苷酸;19-35个核苷酸、20-35个核苷酸;30-45个核苷酸;40-50个核苷酸;50-100个核苷酸;100-300个核苷酸;约500-700个核苷酸;约700-900个核苷酸;约900-1100个核苷酸;约1300-1500个核苷酸;约1500-1700个核苷酸;约1700-1900个核苷酸;约1900-2100个核苷酸;约2100-2300个核苷酸;或约2300-2500个核苷酸。参见,例如国际公开号WO 02/00904。
本披露的发夹分子或双链RNA分子可以具有在该RNA分子的相同部分中发现的多于一个本披露的序列或活性片段或变体或者其互补序列。例如,在嵌合发夹结构中,发夹分子的第一区段包含两个多核苷酸部分,每个具有不同的本披露的序列。例如,从发夹的一个末端开始读,第一区段由来自两个单独基因(A之后是B)的序列构成。该第一区段后面是第二区段,即发夹的环部分。该环区段之后是第三区段,其中发现第一区段中的序列的互补链(B*之后是A*)形成了发夹结构的茎环,该茎包含茎远端的SeqA-A*以及邻近环区的SeqB-B*。
在某些实施例中,该第一和第三区段包含与该第一区段具有至少85%互补性的至少20个核苷酸。在其他实施例中,形成发夹的茎-环结构的第一和第三区段包含具有不成对的核苷酸残基的3′或5’悬垂区域。
在某些实施例中,第一、第二和/或第三区段中使用的序列包含设计为与目的靶标多核苷酸具有足够的序列同一性并且从而具有降低靶标多核苷酸的表达水平的能力的结构域。抑制性RNA转录物的特异性因此通常由沉默元件的这些结构域赋予。因此,在一些实施例中,沉默元件的第一、第二和/或第三区段包含具有至少10、至少15、至少19、至少20、至少21、至少22、至少23、至少24、至少25、至少30、至少40、至少50、至少100、至少200、至少300、至少500、至少1000或1000个以上的核苷酸的结构域,这些核苷酸与靶标多核苷酸共享足够的序列同一性,以允许在合适的细胞中表达时降低靶标多核苷酸的表达水平。在其他实施例中,该结构域介于约15至50个核苷酸之间、约19-35个核苷酸之间、约20-35个核苷酸之间、约25-50个核苷酸之间、约19至75个核苷酸之间、约20至75个核苷酸之间、约40-90个核苷酸之间、约15-100个核苷酸之间、10-100个核苷酸之间、约10至约75个核苷酸之间、约10至约50个核苷酸之间、约10至约40个核苷酸之间、约10至约35个核苷酸之间、约10至约30个核苷酸之间、约10至约25个核苷酸之间、约10至约20个核苷酸之间、约10至约19个核苷酸之间、约50个核苷酸至约100个核苷酸之间、约100个核苷酸至约150个核苷酸之间、约150个核苷酸至约200个核苷酸之间、约200个核苷酸至约250个核苷酸之间、约250个核苷酸至约300个核苷酸之间、约300个核苷酸至约350个核苷酸之间、约350个核苷酸至约400个核苷酸之间、约400个核苷酸至约500个核苷酸之间或更长。在其他实施例中,第一和/或第三区段的长度包含至少10-20个核苷酸、至少10-19个核苷酸、20-35个核苷酸、30-45个核苷酸、40-50个核苷酸、50-100个核苷酸、或约100-300个核苷酸。
在某些实施例中,第一、第二和/或第三区段的结构域与靶标多核苷酸具有100%序列同一性。在其他实施例中,与靶标多核苷酸具有同源性的第一、第二和/或第三区段的结构域与该靶标多核苷酸的区域具有至少50%、60%、70%、80%、85%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%或更高的序列同一性。与靶标多核苷酸互补的第一、第二和/或第三区段的结构域的序列同一性只需足以降低目的靶标多核苷酸的表达即可。参见,例如Chuang和Meyerowitz(2000)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]97∶4985-4990;Stoutjesdijk等人(2002)Plant Physiol.[植物生理学]129∶1723-1731;Waterhouse和Helliwell(2003)Nat.Rev.Genet.[遗传学自然评论]4∶29-38;Pandolfini等人BMC Biotechnology[BMC生物技术学]3∶7,以及美国专利公开号20030175965;其各自通过引用并入本文。用于测定hpRNA构建体至沉默基因体内表达的效率的瞬时测定法已在以下文献中进行描述:Panstruga等人(2003)Mol.Biol.Rep.[分子生物学导报]30:135-140。
第一、第二和/或第三区段与靶标多核苷酸之间共享的互补性的量或第一区段与第三区段(即,发夹结构的茎)之间共有的互补性的量可以根据其基因表达需要被防治的生物体而有所不同。一些生物体或细胞类型可能需要精确配对或100%同一性,而其他生物体或细胞类型可以容忍一些错配。在一些细胞中,例如,靶向序列中的单一核苷酸错配消除了抑制基因表达的能力。在这些细胞中,所披露的抑制盒可以用于靶向突变基因的抑制,例如其转录物包含点突变的癌基因,并因此可使用本发明的方法和组合物特异性地靶向这些癌基因而不改变其余野生型等位基因的表达。在其他生物体中,可以容忍整体序列变异性,只要该序列的某个22nt区域表现出靶标多核苷酸与抑制盒之间的100%同源性即可。
可以使用靶标多核苷酸的任何区域来设计共享足够序列同一性的沉默元件的结构域,以允许发夹转录物的表达来降低靶标多核苷酸的水平。例如,可以将结构域设计为与一种或多种靶标多核苷酸的5′非翻译区、一种或多种靶标多核苷酸的3′非翻译区、一种或多种靶标多核苷酸的外显子区、一种或多种靶标多核苷酸的内含子区以及其任何组合共享序列同一性。在某些实施例中,该沉默元件的结构域与来自靶标序列的约核苷酸1-50、25-75、75-125、50-100、125-175、175-225、100-150、150-200、200-250、225-275、275-325、250-300、325-375、375-425、300-350、350-400、425-475、400-450、-475-525、450-500、525-575、575-625、550-600、625-675、675-725、600-650、625-675、675-725、650-700、725-825、825-875、750-800、875-925、925-975、850-900、925-975、975-1025、950-1000、1000-1050、1025-1075、1075-1125、1050-1100、1125-1175、1100-1200、1175-1225、1225-1275、-1200-1300、1325-1375、1375-1425、1300-1400、1425-1475、1475-1525、1400-1500、1525-1575、1575-1625、1625-1675、1675-1725、1725-1775、1775-1825、-1825-1875、1875-1925、1925-1975、1975-2025、2025-2075、2075-2125、2125-2175、2175-2225、1500-1600、1600-1700、1700-1800、1800-1900、1900-2000的至少约15、16、17、18、19、20、22、25或30个连续核苷酸共享足够的同一性、同源性、或与其互补。在某些情况下,为了优化发夹中使用的siRNA序列,可以使用合成的寡脱氧核糖核苷酸/RNAseH法确定靶标mRNA上易经受RNA沉默的构象中的位点。参见,例如Vickers等人(2003)J.Biol.Chem[生物化学杂志]278:7108-7118和Yang等人(2002)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]99:9442-9447,这些文献通过引用并入本文。这些研究表明,RNase-H-敏感位点与促进有效的siRNA导向的mRNA降解的位点之间具有显著的相关性。
还可对发夹沉默元件进行设计,使得有义序列或反义序列不对应于靶标多核苷酸。在这个实施例中,该反义和有义序列侧翼于如下环序列,该环序列包含对应于靶标多核苷酸的全部或部分的核苷酸序列。因此,是环区决定了RNA干扰的特异性。参见,例如WO 02/00904。
另外,可以通过使用发夹抑制元件实现转录基因沉默(TGS),其中该发夹的反向重复序列与待沉默的靶标多核苷酸的启动子区域共享序列同一性。参见,例如,Aufsatz等人(2002)PNAS 99(增刊4):16499-16506和Mette等人(2000)EMBO J 19(19):5194-5201。
在其他实施例中,该沉默元件可包含小RNA(sRNA)。sRNA可以包含微RNA(miRNA)和短干扰RNA(siRNA)两者(Meister和Tuschl(2004)Nature[自然]431:343-349和Bonetta等人(2004)Nature Methods[自然方法]1:79-86)。miRNA是长度为约19至约24个核糖核苷酸的调控剂,其能高效抑制靶标多核苷酸的表达。参见例如Javier等人(2003)Nature[自然]425:257-263。对于miRNA干扰,可以将该沉默元件设计用来表达形成发夹结构或部分碱基配对的结构的dsRNA分子,该发夹结构或部分碱基配对的结构包含与目的靶标多核苷酸互补的19、20、21、22、23、24或25个核苷酸序列。该miRNA可以经合成产生,或被转录为更长的RNA,该更长的RNA随后裂解,以产生活性miRNA。具体地讲,miRNA可以包含与有义方向上的靶标多核苷酸具有同源性的序列的19个核苷酸,以及与有义序列互补的相应反义序列的19个核苷酸。该miRNA可以是“人造miRNA”或包含miRNA序列的“amiRNA”,该miRNA序列以合成的方式被设计用来使靶标序列沉默。
当表达miRNA时,最终的(成熟的)miRNA以双链体存在于前体主链结构中,这两条链被称为miRNA(将最终与靶标进行碱基配对的链)和miRNA*(星号序列)。已经证明miRNA可以以转基因的方式被表达并且目的靶基因可以被有效地沉默(Highly specific genesilencing by artificial microRNAs in Arabidopsis[在拟南芥中通过人造微RNA进行高度特异性基因沉默],Schwab R,Ossowski S,Riester M,Warthmann N,Weigel D,PlantCell.[植物细胞]2006年5月;18(5):1121-33.Epub 2006年3月10日;以及Expression ofartificial microRNAs in transgenic Arabidopsis thaliana confers virusresistance[人造微RNA在转基因拟南芥中的表达赋予病毒抗性],Niu QW,Lin SS,ReyesJL,Chen KC,Wu HW,Yeh SD,Chua NH,Nat Biotechnol.[自然生物技术]2006年11月;24(11):1420-8,Epub 2006年10月22日.勘误表在Nat Biotechnol.[自然生物技术]2007年2月;25(2):254中)。
用于miRNA干扰的沉默元件包含miRNA一级序列。该miRNA一级序列包含如下DNA序列,该DNA序列具有被环间隔开的miRNA和星号序列,以及侧翼于该区域的对于加工重要的另外的序列。当表达为RNA时,该一级miRNA的这种结构使得允许形成如下发夹RNA结构,该发夹RNA结构可被加工成成熟的miRNA。在一些实施例中,miRNA主链包含基因组的或cDNAmiRNA前体序列,其中所述序列包含在其中插入异源(人造)成熟miRNA和星号序列的天然一级序列。
如本文所使用的,“星号序列”是与miRNA互补的miRNA前体主链内的序列,并与miRNA形成双链体以形成发夹RNA的茎结构。在一些实施例中,该星号序列可以包含与miRNA序列的小于100%的互补性。可替代地,该星号序列可以包含与miRNA序列的至少99%、98%、97%、96%、95%、90%、85%、80%或更低的序列互补性,只要该星号序列与miRNA序列具有足以形成双链结构的互补性即可。在仍另外的实施例中,该星号序列包含与miRNA序列具有1、2、3、4、5或更多个错配的序列并且仍然具有足够的互补性以与miRNA序列形成双链结构,从而导致miRNA的生成和靶标序列的抑制。
该miRNA前体主链可来自任何植物。在一些实施例中,该miRNA前体主链来自单子叶植物。在其他实施例中,该miRNA前体主链来自双子叶植物。在另外的实施例中,该主链来自玉米或大豆。之前已对微RNA前体主链进行了描述。例如,US 20090155910A1(WO 2009/079532)披露了下列大豆miRNA前体主链:156c、159、166b、168c、396b和398b,并且US20090155909A1(WO 2009/079548)披露了下列玉蜀黍miRNA前体主链:159c、164h、168a、169r和396h。
因此,可以改变一级miRNA以允许异源miRNA和星号序列有效插入到miRNA前体主链内。在此类情况下,使用PCR技术将miRNA前体主链的miRNA区段和星号区段替换为设计用来靶向任何目的序列的异源miRNA和异源星号序列,并克隆到表达构建体中。人们认识到,将人造miRNA和星号序列插入该主链的位置处可能会发生改变。用于将miRNA和星号序列插入miRNA前体主链中的详细方法描述于例如美国专利申请20090155909A1和US20090155910A1中。
当设计miRNA序列和星号序列时,可作出各种设计选择。参见,例如,Schwab R等人(2005)Dev Cell[发育细胞]8:517-27。在非限制性实施例中,本文所披露的miRNA序列可具有5′端的“U”、第19个核苷酸位置处的“C”或“G”、以及第10个核苷酸位置处的“A”或“U”。在其他实施例中,miRNA的这种设计使得miRNA具有如用ZipFold算法计算得到的高自由Δ-G(Markham,N.R.和Zuker,M.(2005)Nucleic Acids Res.[核酸研究]33:W577-W581)。任选地,在miRNA的5′部分内可添加一个碱基对改变,以使该序列与靶标序列相差一个核苷酸。
本文披露的方法和组合物使用DNA构建体,所述DNA构建体在转录时“形成”沉默元件(例如dsRNA分子)。所述方法和组合物还可以包含含有编码沉默元件的DNA构建体的宿主细胞。在另一个实施例中,所述方法和组合物还可以包含含有编码沉默元件的DNA构建体的转基因植物。因此,正在被表达的异源多核苷酸不需要自己形成dsRNA,而可以与植物细胞中或摄食后的有害生物肠中的其他序列相互作用,以允许形成dsRNA。例如,可以通过将包含miRNA或siRNA靶标序列的嵌合构建体表达到与待沉默的一个或多个基因的全部或一部分相对应的序列中,来生成能够选择性沉默靶标多核苷酸的嵌合多核苷酸。在这个实施例中,当miRNA或siRNA的靶标与细胞中存在的miRNA相互作用时,“形成”dsRNA。然后所得的dsRNA可以降低待沉默的一个或多个基因的表达水平。参见例如美国申请公开2007-0130653,题目为“Methods and Compositionsfor Gene Silencing[用于基因沉默的方法和组合物]”。可以将该构建体设计为具有内源性miRNA的靶标,或者可替代地,可以在该构建体中使用的异源的和/或合成的miRNA的靶标。如果使用异源的和/或合成的miRNA,可以将其引入与嵌合多核苷酸相同的核苷酸构建体上或单独的构建体上的细胞中。如本文别处所述的,可以用任何方法来引入包含该异源miRNA的构建体。
Ⅳ.变体和片段
“片段”意在是多核苷酸的一部分或者氨基酸序列和因此由其编码的蛋白质的一部分。多核苷酸的片段可以编码保留天然蛋白的生物活性的蛋白质片段。可替代地,可用作沉默元件的多核苷酸片段不必编码保留生物活性的片段蛋白质。因此,核苷酸序列的片段的范围为至少约10个核苷酸、约15个核苷酸、约16个核苷酸、约17个核苷酸、约18个核苷酸、约19个核苷酸、约20个核苷酸、约22个核苷酸、约50个核苷酸、约75个核苷酸、约100个核苷酸、200个核苷酸、300个核苷酸、400个核苷酸、500个核苷酸、600个核苷酸、700个核苷酸并且最多且包括比所使用的全长多核苷酸少一个核苷酸。可替代地,核苷酸序列的片段的范围可以为SEQ ID NO.:1-53或107-254中任一项、或者其变体和片段、及其互补序列的1-50、25-75、75-125、50-100、125-175、175-225、100-150、100-300、150-200、200-250、225-275、275-325、250-300、325-375、375-425、300-350、350-400、425-475、400-450、475-525、450-500、525-575、575-625、550-600、625-675、675-725、600-650、625-675、675-725、650-700、725-825、825-875、750-800、875-925、925-975、850-900、925-975、975-1025、950-1000、1000-1050、1025-1075、1075-1125、1050-1100、1125-1175、1100-1200、1175-1225、1225-1275、1200-1300、1325-1375、1375-1425、1300-1400、1425-1475、1475-1525、1400-1500、1525-1575、1575-1625、1625-1675、1675-1725、1725-1775、1775-1825、1825-1875、1875-1925、1925-1975、1975-2025、2025-2075、2075-2125、2125-2175、2175-2225、1500-1600、1600-1700、1700-1800、1800-1900、1900-2000。测定期望的沉默元件的活性的方法在本文其他地方进行了描述。
“变体”意指基本上相似的序列。对于多核苷酸,变体包含在天然多核苷酸中的一个或多个内部位点处的一个或多个核苷酸的缺失和/或添加,和/或在天然多核苷酸中的一个或多个位点处的一个或多个核苷酸的取代。可用作沉默元件的多核苷酸的变体将保留降低靶标多核苷酸表达的能力,并且在一些实施例中,因此能防治目的植物昆虫有害生物。如在此使用,“天然”多核苷酸或多肽分别包含天然存在的核苷酸序列或氨基酸序列。对于多核苷酸,保守的变体包括如下那些序列,由于遗传密码的简并性而,该序列编码所披露的多肽之一的氨基酸序列。变体多核苷酸还包括通过合成衍生的多核苷酸,诸如那些例如通过使用定点诱变产生但继续保留所需活性的多核苷酸。通常,如通过本文别处所描述的序列比对程序和参数确定的,具体的经披露的多核苷酸(即,沉默元件)的变体将与该具体的多核苷酸具有至少约40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%或更高的序列同一性。
具体的经披露的多核苷酸(即参照多核苷酸)的变体还可通过比较由变体多核苷酸所编码的多肽与由该参照多核苷酸所编码的多肽之间的序列同一性百分数来进行评价。可以使用本文别处描述的序列比对程序和参数计算任何两个多肽之间的百分比序列同一性。当通过比较由它们编码的两个多肽共有的序列同一性百分数来评估所使用的给定的所披露的多核苷酸对时,两个经编码的多肽之间的序列同一性百分数为至少约40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%或更高的序列同一性。
以下术语用于描述两个或更多个多核苷酸或多肽之间的序列关系:(a)“参照序列′,(b)“比较窗口”,(c)“序列同一性”以及(d)“序列同一性百分数”。
(a)如在此使用,“参照序列”是用作序列比较的基础的所定义的序列。参照序列可以是指定序列的子集或整体;例如,作为全长cDNA或基因序列的区段、或完整的cDNA或基因序列。
(b)如本文所用,“比较窗口”参照了多核苷酸序列的连续并指定的区段,其中与用于两种多核苷酸的最佳比对的参照序列(其不包括添加或缺失)相比,比较窗口中的多核苷酸序列可能包括添加或缺失(即空位)。通常,比较窗口的长度为至少20个连续核苷酸,并且任选地可以是30、40、50、100个或更长。本领域技术人员应当理解,由于多核苷酸序列中含有缺口,为了避免与参照序列的高相似性,典型地引入缺口罚分,并且将其从匹配数中减去。
除非另行说明,否则本文中提供的序列同一性/相似性值是指使用采用以下参数的GAP版本10获得的值:核苷酸序列的%同一性和%相似性采用GAP权重50和长度权重3,以及nwsgapdna.cmp评分矩阵;氨基酸序列的%同一性和%相似性采用GAP权重8和长度权重2,以及BLOSUM62评分矩阵;或其任何等效程序。“等效的程序”是指任何序列比较程序,该程序对于任何两个所讨论的序列产生一个比对,当与GAP版本10所产生的对应的比对相比较时,该比对具有相同的核苷酸或氨基酸残基配对以及相同的序列同一性百分比。
(c)如本文所用,在两个多核苷酸或多肽序列的上下文中的“序列同一性”或“同一性”是指,当在指定比较窗口上比对最大对应性时,两个序列中的相同残基。当使用关于蛋白质的序列同一性百分比时,认识到不相同的残基位置通常相差保守氨基酸取代,其中氨基酸残基被具有相似化学性质(例如电荷或疏水性)的其他氨基酸残基取代,并且因此不改变分子的功能性质。当序列在保守取代方面不同时,可以向上调节序列同一性百分比,以校正该取代的保守性质。相差这些保守取代的序列被称为具有“序列相似性”或“相似性”。用于进行该调整的方法是本领域技术人员所熟知的。典型地,这涉及作为部分而不是完全错配对保守取代打分,从而提高百分比序列同一性。因此,例如,当相同的氨基酸得分为1,并且非保守取代的得分为零时,保守取代的得分在零和1之间。计算保守取代的评分,例如,如在程序PC/GENE(智能遗传学(Intelligenetics),山景城,加利福尼亚州)中实现的。
(d)如本文所用,“序列同一性百分比”意指在比较窗口上比较两个最佳比对序列所确定的值,其中与参照序列(其不包含添加或缺失)相比,该比较窗中的多核苷酸序列部分可以包含添加或缺失(即空位),以进行这两个序列的最佳比对。通过以下方式计算该百分比:确定在两个序列中出现相同核酸碱基或氨基酸残基的位置的数目以产生匹配位置的数目,将匹配位置的数目除以比较窗口中的位置的总数目,然后将该结果乘以100以产生序列同一性百分比。
进一步提供了用于从SEQ ID NO.:1-53或107-254中所示的靶标多核苷酸、或者其变体和片段、及其互补序列中识别沉默元件的方法。这样的方法包括获得SEQ ID NO.:1-53或107-254中任一项、或者其变体和片段、及其互补序列的的候选片段,该候选片段具有足够的长度以至于能充当沉默元件,并且从而降低靶标多核苷酸的表达和/或防治期望的有害生物;在合适的表达盒中表达所述候选多核苷酸片段以产生候选沉默元件,并确定所述候选多核苷酸片段具有沉默元件的活性,并且从而降低靶标多核苷酸的表达和/或防治期望的有害生物。鉴于本文提供的教导,基于用于抑制的期望的途径来识别此类候选片段的方法是已知的。例如,可以采用各种生物信息学程序来识别可以被用来产生沉默元件的靶标多核苷酸的区域。参见,例如,Elbahir等人(2001)Genesand Development[基因与发育]15∶188-200,Schwartz等人(2003)Cell[细胞]115∶199-208,Khvorova等人(2003)Cell[细胞]115∶209-216。还参见,在Whitehead网站(jura.wi.mit.edu/bioc/siRNAext/)的siRNA,其计算正义和反义siRNA的结合能。还参见网址genscript.com/ssl-bin/app/rnai?op=known;来自英杰公司(Invitrogen)和金斯瑞公司(GenScript)siRNA构建体生成器(siRNAConstruct Builder)的Block-iTTMRNAi设计器(Block-iTTM RNAi designer)。在各个方面,应当理解,术语“......SEQ ID NO.:1-53或107-254,或者其变体或片段,或者其互补序列......”意在表示,本披露的序列包含SEQ ID NO.:1-53或107-254,和/或SEQ ID NO.:1-53或107-254的片段,和/或SEQ ID NO.:1-53或107-254的变体,和/或SEQ ID NO.:1-53或107-254、SEQ ID NO.:1-53或107-254的变体、和/或SEQ ID NO.:1-53或107-254的片段的互补序列,单独地(或)或包含一些或所有列出的序列。
V.DNA构建体
术语“多核苷酸”的用途并不限于包含DNA的多核苷酸。本领域普通技术人员将认识到,多核苷酸可以包含核糖核苷酸和核糖核苷酸与脱氧核糖核苷酸的组合。这种脱氧核糖核苷酸和核糖核苷酸既包括天然存在的分子也包括合成的类似物。所披露的多核苷酸还涵盖所有形式的序列,包括但不限于单链形式、双链形式、发夹结构、茎-环结构等。
编码沉默元件或在某些实施例中的、在披露的方法和组合物中使用的多核苷酸可以在表达盒中提供以便在目的植物或生物体中表达。已经认识到,可以使用多个沉默元件,该多个沉默元件包括多个相同沉默元件、多个靶向靶标序列不同区域的沉默元件、或多个来自不同靶标序列的沉默元件。在这个实施例中,认识到每个沉默元件可以由单个或单独的盒、DNA构建体或载体编码。如所讨论的,设想了提供该沉默元件的任何方式。可以用包含编码一个或多个沉默元件的DNA的单个的盒或者编码沉默元件的分离的盒来转化植物或植物细胞,以转化植物或者植物细胞或宿主细胞。同样地,用一个组分转化的植物可以随后用第二组分进行转化。还可以通过有性杂交将编码沉默元件的一个或多个DNA构建体聚在一起。也就是说,包含一种组分的第一种植物与包含第二种组分的第二植物杂交。杂交所产生的子代植物将包含这两种组分。
该表达盒可以包括有效地连接至本发明的多核苷酸的5′和3′调控序列。“有效地连接”旨在表示两个或更多个元件之间的功能性连接。例如,本发明的多核苷酸与调节序列(即,启动子)之间的有效连接是可使本文披露的多核苷酸得以表达的功能性连接。有效地连接的元件可以是连续的或非连续的。当用于指两个蛋白质编码区域的连接时,有效地连接意在是这些编码区域处于相同的阅读框中。该盒可以另外地含有至少一个待共转化成生物体的另外的多核苷酸。可替代地,可以在多个表达盒上提供另外的一种或多种多肽。提供的表达盒可以具有多个限制性位点和/或重组位点,用于将多核苷酸插入到调节区的转录调控之下。该表达盒可另外包含选择性标记基因。
该表达盒可以包括转录的5′-3′方向、转录和翻译起始区(即启动子)、编码本发明的方法和组合物中使用的沉默元件的多核苷酸、以及在植物中起作用的转录和翻译终止区(即,终止区)。在其他实施例中,从抑制盒表达双链RNA。这样的盒可以包含驱动有效地连接的沉默元件的转录的两个趋同启动子。“趋同启动子”是指定向于编码该沉默元件的、有效地连接的多核苷酸任一末端的启动子,使得每个启动予以相反方向驱动该沉默元件的转录,产生两个转录物。在此类实施例中,趋同启动子允许正义和反义链的转录,并且从而允许dsRNA的形成。这种盒还可以包含驱动编码该沉默元件的一个或多个有效地连接的多核苷酸的转录的两个趋异启动子。“趋异启动子”是指彼此以相反方向定向的启动子,以相反的方向驱动编码该沉默元件的一个或多个多核苷酸的转录。在此类实施例中,趋异启动子允许正义和反义链的转录并允许dsRNA的形成。在此类实施例中,趋异启动子还允许至少两个单独发夹RNA的转录。在另一个实施例中,构建体中存在一个盒,该盒包含受到在相同定向上的两个单独启动子控制的、编码该沉默元件的两个或更多个多核苷酸。在另一个实施例中,构建体中存在在相同定向上的两个或更多个单独的盒,每个盒包含受到启动子控制的、编码该沉默元件的至少一个多核苷酸。
本文披露的调控区(即,启动子、转录调控区和翻译终止区)和/或多核苷酸对于宿主细胞而言或彼此之间可以是天然的/相似的。可替代地,本文披露的调控区和/或多核苷酸对于宿主细胞或彼此之间可以是异源的。如本文所使用的,关于序列的“异源的”是指该序列源于外来物种,或者,如果源于相同物种的话,则是通过蓄意人为干预从组合物和/或基因组基因座中的天然形式进行实质性修饰得到的序列。例如,有效地连接至异源多核苷酸的启动子来自与从其衍生该多核苷酸的物种不同的物种,或者,如果来自相同/类似的物种,那么一方或双方基本上由它们的原来形式和/或基因组基因座修饰得到,或者该启动子不是被有效地连接的多核苷酸的天然启动子。如本文所使用的,嵌合基因包含与转录起始区有效地连接的编码序列,该转录起始区对于该编码序列是异源的。
终止区可以对于转录起始区而言是天然的,可以对于编码沉默元件的有效地连接的多核苷酸而言是天然的,可以对于植物宿主而言是天然的,或者可以衍生自对于启动子、编码该沉默元件的多核苷酸、植物宿主或其任何组合而言别的来源(即外来的或异源的)。方便的终止区可获自根瘤农杆菌(A.tumefaciens)的Ti质粒,诸如章鱼碱合酶和胭脂碱合酶终止区。还参见Guerineau等人,(1991)Mol.Gen.Genet.[分子遗传学和普通遗传学]262∶141-144;Proudfoot(1991)Cell[细胞]64∶671-674;Sanfacon等人(1991)Genes Dev.[基因与发育]5∶141-149;Mogen等人(1990)Plant Cell[植物细胞]2∶1261-1272;Munroe等人(1990)Gene[基因]91∶151-158;Ballas等人(1989)Nucleic Acids Res.[核酸研究]17∶7891-7903;以及Joshi等人(1987)Nucleic Acids Res.[核酸研究]15∶9627-9639。
已知有另外的序列修饰能增强细胞宿主中的基因表达。这些包括:消除编码假的聚腺苷酸化信号和外显子-内含子剪接位点信号的序列、转座子样重复序列、及可能不利于基因表达的其他经充分表征的序列。可将序列的G-C含量调整至通过参照宿主细胞中表达的已知基因而计算出的给定细胞宿主的平均水平。当可能时,修饰序列以避免出现预测的发夹二级mRNA结构。
在制备表达盒时,可以操作各种DNA片段,以提供处于适当方向以及合适时,处于适当阅读框中的DNA序列。为此,可采用衔接子(adapter)或接头以连接DNA片段,或可以涉及其他操作以提供方便的限制位点、移除多余的DNA、移除限制位点等。出于这个目的,可以涉及体外诱变、引物修复、限制性酶切(restriction)、退火、再取代(例如转换和颠换)。
多种启动子可用于本发明的实践中。可以基于期望的结果来选择启动子。核酸可以与组成型启动子、组织偏好性启动子、诱导型启动子或其他启动子组合用于在宿主生物体中的表达。
此类组成型启动子包括,例如Rsyn7启动子的核心启动子和其他在WO99/43838和美国专利号6,072,050中披露的组成型启动子;核心CaMV 35S启动子(Odell等人,(1985)Nature[自然]313∶810-812);稻肌动蛋白(McElroy等人,(1990)Plant Cell[植物细胞]2∶163-171);泛素(Christensen等人,(1989)Plant Mol.Biol.[植物分子生物学]12∶619-632以及Christensen等人,(1992)Plant Mol.Biol.[植物分子生物学]18:675-689);pEMU(Last等人,(1991)Theor.Appl.Genet.[理论与应用遗传学]81∶581-588);MAS(Velten等人,(1984)EMBO J.[欧洲分子生物学学会杂志]3:2723-2730);ALS启动子(美国专利号5,659,026)等。其它组成型启动子包括例如美国专利号5,608,149;5,608,144;5,604,121;5,569,597;5,466,785;5,399,680;5,268,463;5,608,142;和6,177,611。
取决于所希望的结果,从诱导型启动子表达基因可能是有益的。还可以采用诱导型启动子,例如病原体诱导型启动子。这些启动子包括来自病程相关蛋白(PR蛋白)的那些启动子,其在被病原体感染后收到诱导;例如,PR蛋白、SAR蛋白、β-1,3-葡聚糖酶、几丁质酶等。参见,例如Redolfi等人(1983)Neth.J.Plant Pathol.[荷兰植物病理学杂志]89∶245-254;Uknes等人(1992)Plant Cell[植物细胞]4∶645-656;以及Van Loon(1985)PlantMol.Virol.[植物分子病毒学]4:111-116。还参见WO 99/43819。
此外,由于病原体通过伤口或昆虫损伤找到进入植物的入口,伤口诱导型启动子可以用于本发明的构建中。这种伤口诱导型启动子包括马铃薯蛋白酶抑制剂(pin II)基因(Ryan(1990)Ann.Rev.Phytopath.[植物病理学年鉴]28∶425-449;Duan等人(1996)NatureBiotechnology[自然生物技术]14∶494-498);wun1和wun2,美国专利号5,428,148;win1和win2(Stanford等人(1989)Mol.Gen.Genet.[分子和普通遗传学]215∶200-208);系统素(McGurl等人(1992)Science[科学]225∶1570-1573);WIP1(Rohmeier等人(1993)PlantMol.Biol.[植物分子生物学]22:783-792;Eckelkamp等人(1993)FEBS Letters[欧洲生化学会联盟通讯]323:73-76);MPI基因(Corderok等人(1994)PlantJ.[植物杂志]6(2):141-150);等等。
此外,可以在实施例的方法和核苷酸构建体中使用病原诱导型启动子。这些病原体诱导型启动子包括来自病程相关蛋白(PR蛋白)的那些启动子,其在被病原体感染后收到诱导;例如,PR蛋白、SAR蛋白、β-1,3-葡聚糖酶、几丁质酶等。参见,例如Redolfi等人(1983)Neth.J.Plant Pathol.[荷兰植物病理学杂志]89:245-254;Uknes等人(1992)Plant Cell[植物细胞]4:645-656;以及Van Loon(1985)Plant Mol.Virol.[植物分子病毒学]4:111-116。还参见WO 99/43819。
目的是在病原体侵染部位处或附近局部表达的启动子。参见,例如,Marineau等人,(1987)Plant Mol.Biol.[植物分子生物学]9:335-342;Matton等人,(1989)MolecularPlant-Microbe Interactions[分子植物-微生物相互作用]2:325-331;Somsisch等人,(1986)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]83:2427-2430;Somsisch等人,(1988)Mol.Gen.Genet.[分子遗传和基因组学]2:93-98以及Yang,(1996)Proc.Natl.Acad.Sci.[美国国家科学院院刊]USA 93:14972-14977。还参见Chen等人,(1996)Plant J.[植物杂志]10:955-966;Zhang等人,(1994)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]91:2507-2511;Warner等人,(1993)Plant J.[植物杂志]3:191-201;Siebertz等人,(1989)Plant Cell[植物细胞]1:961-968;美国专利号5,750,386(可诱导线虫的)。特定的目的是玉米PRms基因的诱导型启动子,其表达是由病原体串珠镰刀菌(Fusarium moniliforme)诱导的(参见例如Cordero等人,(1992)Physiol.Mol.PlantPath.[生理学与分子植物病理学]41:189-200)。
可以使用化学调节型启动予以通过应用外源化学调节剂来调节植物中的基因表达。取决于目标,在应用化学品诱导基因表达的情况下启动子可以是化学诱导型启动子,或者在应用化学品阻抑基因表达的情况下启动子可以是化学阻抑型启动子。化学诱导型启动子是本领域已知的,并且包括但不限于由苯磺酰胺除草剂安全剂激活的玉米In2-2启动子、由用作萌前除草剂的疏水亲电子化合物激活的玉米GST启动子、以及由水杨酸激活的烟草PR-1a启动子。其他目的化学调节的启动子包括类固醇应答性启动子(参见,例如,糖皮质激素诱导型启动子(Schena等人,(1991)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]88:10421-10425和McNellis等人,(1998)Plant J[植物杂志]14(2):247-257)以及四环素诱导型和四环素阻抑型启动子(参见例如Gatz等人,(1991)Mol Gen Genet[分子遗传和基因组学]227∶229-237以及美国专利号5,814,618和5,789,156)。
组织偏好性启动子可以用于靶向特定植物组织内的增强的表达。组织偏好性启动子包括Yamamoto等人(1997)PlantJ.[植物杂志]12(2):255-265;Kawamata等人(1997)Plant CellPhysiol.[植物细胞生理学]38(7):792-803;Hansen等人(1997)Mol.GenGenet.[分子遗传学和普通遗传学]254(3):337-343;Russell等人(1997)Transgenic Res.[转基因研究]6(2):157-168;Rinehart等人(1996)Plant Physiol.[植物生理学]112(3):1331-1341;Van Camp等人(1996)Plant Physiol.[植物生理学]112(2):525-535;Canevascini等人(1996)Plant Physiol.[植物生理学]112(2):513-524;Yamamoto等人(1994)Plant Cell Physiol.[植物细胞生理学]35(5):773-778;Lam(1994)ResultsProbl.Cell Differ.[细胞分化的结果和问题]20:181-196;Orozco等人(1993)PlantMolBiol.[植物分子生物学]23(6):1129-1138;Matsuoka等人(1993)ProcNatl.Acad.Sci.USA[美国国家科学院院刊]90(20):9586-9590;和Guevara-Garcia等人(1993)Plant J.[植物杂志]4(3):495-505。如果需要,此类启动子可以被修饰用于弱表达。
叶偏好性启动子是本领域已知的。参见,例如,Yamamoto等人,(1997)Plant J.[植物杂志]12(2):255-265;Kwon等人,(1994)Plant Physiol.[植物生理学]105∶357-67;Yamamoto等人,(1994)Plant Cell Physiol.[植物细胞生理学]35(5):773-778;Gotor等人,(1993)Plant J.[植物杂志]3∶509-18;Orozco等人,(1993)Plant Mol.Biol[植物细胞生理学].23(6):1129-1138以及Matsuoka等人,(1993)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]90(20):9586-9590。
根偏好性启动子是已知的,并且可以选自来自文献的许多可获得的启动子,或从各种相容物种中重新分离。参见,例如,Hire等人,(1992)Plant Mol.Biol.[植物分子生物学]20(2):207-218(大豆根特异性谷氨酰胺合成酶基因);Keller和Baumgartner(1991)Plant Cell[植物细胞]3(10):1051-1061(法国菜豆的GRP 1.8基因中的根特异性控制元件);Sanger等人,(1990)Plant Mol.Biol.[植物分子生物学]14(3):433-443(根癌农杆菌的甘露碱合酶(MAS)的根特异性启动子);以及Miao等人,(1991)Plant Cell[植物细胞]3(1):11-22(编码细胞溶质谷氨酰胺合成酶(GS)的全长cDNA克隆,其在大豆根和根结节中表达)。还参见Bogusz等人(1990)Plant Cell[植物细胞]2(7):633-641,该文献描述了分离自血红蛋白基因的两个根特异性启动子,该血红蛋白基因来自固氮非豆科作物山黄麻(Parasponia andersonii)和相关的非固氮非豆科作物山油麻(Trema tomentosa)。这些基因的启动子与β-葡糖醛酸糖苷酶报告基因连接,并且被引入非豆科作物烟草(Nicotianatabacum)和豆科作物百脉根(Lotus corniculatus)两者中,并且在两种情况下都保留了根特异性启动子活性。Leach和Aoyagi(1991)描述了他们对发根土壤杆菌的高表达的rolC和rolD根诱导性基因的启动子的分析(参见Plant Science[植物科学](Limerick)79(1):69-76)。他们得出结论,增强子和组织偏好性DNA决定簇在这些启动子中是解离的。Teeri等人(1989)对lacZ使用基因融合来表明,编码章鱼碱合酶的土壤杆菌T-DNA基因在根尖的表皮中特别活跃,并且TR2′基因在完好的植物中是根特异性的,并且受到叶组织中的伤害的刺激,特别合适的具有与杀昆虫或杀幼虫基因一起使用的特性的组合(参见EMBO J.[欧洲分子生物学学会杂志]8(2):343-350)。融合至nptII(新霉素磷酸转移酶II)上的TR1′基因显示出相似的特性。另外的根偏好性启动子包括VfENOD-GRP3基因启动子(Kuster等人(1995)Plant Mol.Biol.[植物分子生物学]29(4):759-772);和rolB启动子(Capana等人(1994)Plant Mol.Biol.[植物分子生物学]25(4):681-691。还参见美国专利号5,837,876;5,750,386;5,633,363;5,459,252;5,401,836;5,110,732;和5,023,179。
“种子偏好性”启动子包括“种子特异性”启动子(在种子发育期间有活性的那些启动子如种子贮藏蛋白的启动子)以及“种子发芽性”启动子(在种子发芽期间有活性的那些启动子)。参见Thompson等人,(1989)BioEssays[生物学分析]10∶108。这样的种子优选的启动子包括但不限于Cim1(细胞分裂素诱导的信息);cZ19B1(玉米19kDa玉米蛋白);和milps(肌醇-1-磷酸盐合酶)(参见美国专利号6,225,529,通过引用并入本文)。γ-玉米蛋白和Glob-1是胚乳特异性启动子。对于双子叶植物,种子特异性启动子包括但不限于:菜豆β-菜豆素、油菜籽蛋白(napin)、β-伴大豆球蛋白、大豆凝集素、十字花科蛋白(cruciferin)等。对于单子叶植物,种子特异性启动子包括但不限于:玉蜀黍15kDa玉蜀黍蛋白、22kDa玉蜀黍蛋白、27kDa玉蜀黍蛋白、g-玉蜀黍蛋白、蜡质、收缩素1、收缩素2、球蛋白1等。还参见WO 00/12733,其中披露了来自end1和end2基因的种子偏好性启动子。在特定组织中具有“偏好性”表达的启动子在该组织中比在至少一种其他植物组织中更大程度地表达。一些组织优先的启动子几乎专门在特定组织中表达。
在实施例中,植物表达性启动子是血管特异性启动子,例如韧皮部特异性启动子。如本文所使用的,“血管特异性”启动子是至少在血管细胞中表达的启动子或优先在血管细胞中表达的启动子。血管特异性启动子的表达不必仅在血管细胞中,其他细胞类型或组织中的表达也是可能的。如本文所使用的,“韧皮部特异性启动子”是至少在韧皮部细胞中表达的植物可表达性启动子,或优先在韧皮部细胞中表达的启动子。
韧皮部特异性启动子的表达不需要仅在韧皮部细胞中,在其他细胞类型或组织(例如木质部组织)中的表达也是可能的。在本发明的一个实施例中,韧皮部特异性启动子是至少在韧皮部细胞中表达的植物可表达性启动子,其中与韧皮部细胞中的表达相比,非韧皮部细胞中的表达更有限(或不存在)。根据本发明,合适的血管特异性或韧皮部特异性启动子的实例包括但不限于选自由以下各项组成的组的启动子:SCSV3、SCSV4、SCSV5和SCSV7启动子(Schunmann等人(2003)Plant Functional Biology[植物生物学功能]30∶453-60);发根土壤杆菌的rolC基因启动子(Kiyokawa等人(1994)PlantPhysiology[植物生理学]104∶801-02;Pandolfini等人(2003)BioMedCentral(BMC)Biotechnology[生物医学中心(BMC)生物技术]3∶7,(www.biomedcentral.com/1472-6750/3/7);Graham等人(1997)Plant Mol.Biol.[植物分子生物学]33:729-35;Guivarc′h等人(1996);Almon等人(1997)Plant Physiol.[植物生理学]115∶1599-607);发根土壤杆菌的rolA基因启动子(Dehio等人(1993)Plant Mol.Biol.[植物分子生物学]23∶1199-210);根癌农杆菌T-DNA基因5的启动子(Korber等人(1991)EMBO J.[欧洲分子生物学学会杂志]10∶3983-91);稻蔗糖合酶RSs1基因启动子(Shi等人(1994)J.Exp.Bot.[实验植物学杂志]45∶623-31);CoYMV或鸭跖草黄斑驳杆状病毒(Commelina yellow mottle badnavirus)启动子(Medberry等人(1992)Plant Cell[植物细胞]4∶185-92;Zhou等人(1998)Chin.J.Biotechnol.[中国生物技术杂志]14∶9-16);CFDV或椰子叶面腐败病毒启动子(Rohde等人(1994)Plant Mol.Biol.[植物分子生物学]27∶623-28;Hehn和Rhode(1998)J.Gen.Virol.[普通病毒学杂志]79∶1495-99);RTBV或东格鲁水稻杆状病毒(rice tungro bacilliform virus)启动子(Yin和Beachy(1995)Plant J.[植物杂志]7∶969-80;Yin等人(1997)Plant J.[植物杂志]12∶1179-80);豌豆谷氨酰胺合酶GS3A基因(Edwards等人(1990)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]87∶3459-63;Brears等人(1991)Plant J.[植物杂志]1∶235-44);马铃薯转化酶基因的inv CD111和inv CD141启动子(Hedley等人(2000)J.Exp.Botany[实验植物学杂志]51∶817-21);从拟南芥中分离的启动子显示在烟草中具有韧皮部特异性表达(Kertbundit等人(1991)Proc.Natl.Acad. Sci.USA[美国国家科学院院刊]88:5212-16);VAHOX1启动子区(Tornero等人(1996)Plant J.[植物杂志]9:639-48);豌豆细胞壁转化酶基因启动子(Zhang等人(1996)Plant Physiol.[植物生理学]112:1111-17);与美国公开专利申请20030106097,来自胡萝卜的酸性转化酶基因启动子的几丁质酶相关的内源性棉花蛋白的启动子(Ramloch-Lorenz等人(1993)The Plant J.[植物学杂志]4:545-54);硫酸转运蛋白基因Sultr1的启动子;3(Yoshimoto等人(2003)Plant Physiol.[植物生理学]131:1511-17);蔗糖合酶基因的启动子(Nolte和Koch(1993)Plant Physiol.[植物生理学]101:899-905);以及烟草蔗糖转运蛋白基因的启动子(Kuhn等人(1997)Science[科学]275-1298-1300)。
可能的启动子还包括洋李甙水解酶(PH DL1.4PRO)的黑樱桃启动子(美国专利号6,797,859),来自黄瓜和稻的硫氧还蛋白H启动子(Fukuda A等人(2005),Plant CellPhysiol.[植物细胞生理学]46(11):1779-86),稻(RSs1)(Shi,T.Wang等人(1994).J.Exp.Bot.[实验植物学杂志]45(274):623-631)和玉米蔗糖合酶-1启动子(Yang.,N-S.等人(1990)PNAS87:4144-4148),来自南瓜的PP2启动子(Guo,H.等人(2004)TransgenicResearch[转基因研究]13:559-566),SUC2启动子(Truernit,E.等人(1995)Planta[植物]196(3):564-70),SAM-1(S-腺苷甲硫氨酸合成酶)(Mijnsbrugge KV.等人(1996)PlantCell.Physiol.[植物细胞生物学]37(8):1108-1115),以及水稻东格鲁杆状病毒(RTBV)启动子(Bhattacharyya-Pakrasi等人(1993)PlantJ.[植物杂志]4(1):71-79)。
当希望低水平表达时,可使用弱启动子。通常,如本文使用的,术语“弱启动子”是指以低水平驱动编码序列表达的启动子。低水平表达旨在约1/1000转录物至约1/100,000转录物至约1/500,000转录物的水平。可替代地,应当认识到,术语“弱启动子”还涵盖仅在少数细胞中驱动表达但不在其他细胞中表达以给出完全低表达水平的启动子。当启动予以不可接受的高水平驱动表达时,可以删除或修饰部分启动子序列以降低表达水平。
此类弱组成型启动子包括,例如:Rsyn7启动子的核心启动子(WO99/43838和美国专利号6,072,050)、核心35S CaMV启动子等。其他组成型启动子包括,例如以下专利文献中所披露的那些:美国专利号5,608,149;5,608,144;5,604,121;5,569,597;5,466,785;5,399,680;5,268,463;5,608,142;和6,177,611。
该表达盒还可以包含用于选择经转化细胞的可选标记基因。利用可选标记基因用于选择经转化的细胞或组织。标记基因包括编码抗生素抗性的基因,如编码新霉素磷酸转移酶II(NEO)和潮霉素磷酸转移酶(HPT)的基因,以及赋予除草化合物(如草铵膦、溴苯腈、咪唑啉酮和2,4-二氯苯氧乙酸(2,4-D))抗性的基因。另外的可选标记包括表型标记,例如β-半乳糖苷酶和荧光蛋白,如绿色荧光蛋白(GFP)(Su等人(2004)Biotechnol Bioeng[生物技术与生物工程]85:610-9和Fetter等人(2004)Plant Cell[植物细胞]16:215-28)、青色荧光蛋白(CYP)(Bolte等人(2004)J.Cell Science[细胞科学杂志]117∶943-54和Kato等人(2002)Plant Physiol[植物生理学]129:913-42)和黄色荧光蛋白(来自Evrogen的PhiYFPTM,参见Bolte等人(2004)J.Cell Science[细胞科学杂志]117∶943-54)。对于另外的可选标记,一般参见Yarranton(1992)Curr.Opin.Biatech.[生物技术新见]3∶506-511;Christopherson等人(1992)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]89∶6314-6318;Yao等人(1992)Cell[细胞]71∶63-72;Reznikoff(1992)Mo,.Microbiol.[分子微生物学]6∶2419-2422;Barkley等人(1980)The Operon[操纵子]中,第177-220页;Hu等人(1987)Cell[细胞]48∶555-566;Brown等人(1987)Cell[细胞]49:603-612;Figge等人(1988)Cell[细胞]52:713-722;Deuschle等人(1989)Proc.Nail.Acad. Sci.USA[美国国家科学院院刊]86:5400-5404;Fuerst等人(1989)Proc.Nail.Acad. Sci.USA[美国国家科学院院刊]86∶2549-2553;Deuschle等人(1990)Science[科学]248∶480-483;Gossen(1993)博士学位论文,德国海德堡大学;Reines等人(1993)Proc.Natl.Acad. Sci.USA[美国国家科学院院刊]90∶1917-1921;Labow等人(1990)Mol.Cell.Bio l.[分子细胞生物学]10:3343-3356;Zambretti等人(1992)Proc.Natl.Acad. Sci.USA[美国国家科学院院刊]89∶3952-3956;Baim等人(1991)Proc.Natl.Acad. Sci.USA[美国国家科学院院刊]88∶5072-5076;Wyborski等人(1991)NucleicAcids Res.[核酸研究]19:4647-4653;Hillenand-Wissman(1989)Topics Mol.Struc.Biol.[热点分子结构生物学]10:143-162;Degenkolb等人(1991)Antimicrob.Agents Chemother.[抗微生物剂]35:1591-1595;Kleinschnidt等人(1988)Biochemistry[生物化学]27:1094-1104;Bonin(1993)博士学位论文,德国海德堡大学;Gossen等人(1992)Proc.Natl.Acad. Sci.USA[美国国家科学院院刊]89:5547-5551;Oliva等人(1992)Antimicrob.Agents Chemother.[抗微生物剂]36:913-919;Hlavka等人(1985)Handbook of Experimental Pharmacology[实验药理学手册],第78卷(Springer-Verlag,Berlin[柏林施普林格出版社]);Gill等人,(1988)Nature[自然]334:721-724。以上可选标记基因的列表并不意指是限制性的。任何可选标记基因都可以与本文所述的组合物和方法一起使用。
VI.包含沉默元件的组合物
可以提供包含沉默元件的多核苷酸中的一种或多种作为植物、植物部分、种子、植物昆虫有害生物或耕作区的外用组合物,诸如喷雾剂或粉剂。在另一个实例中,用DNA构建体或表达盒转化植物用于至少一个沉默元件的表达。在任一组合物中,沉默元件在被有害生物摄取时可降低靶标有害生物序列的水平并从而防治该有害生物(即,鞘翅目植物有害生物,包括叶甲属植物有害生物,诸如玉米根萤叶甲、北方玉米根虫、墨西哥玉米根虫、南美叶甲或斑点黄瓜甲虫)。已经认识到,该组合物可以包含如下细胞(诸如植物细胞或细菌细胞),在该细胞中,编码沉默元件的多核苷酸稳定地被并入到基因组中并有效地连接到在细胞中有活性的启动子上。还涵盖包含细胞(一些表达至少一个沉默元件的细胞)混合物的组合物。在其他实施例中,包含沉默元件的组合物不包含于细胞中。在此类实施例中,可将该组合物施用到植物昆虫有害生物所栖息的区域。在一个实施例中,将该组合物外用地施用于植物(即,通过喷洒田间或种植区域)来保护植物免受有害生物侵害。以这种方式施用核苷酸的方法是本领域技术人员已知的。
还可以将本文披露的组合物配制成诱饵。在此实施例中,这些组合物包含增加该组合物对有害生物的吸引力的食品或引诱剂。
包含沉默元件的组合物可在农业上合适的和/或环境上可接受的载体中进行配制。此类载体可以为待处理的动物、植物或环境可耐受的任何材料。此外,此类载体必须使得该组合物在防治植物昆虫有害生物时仍然有效。此类载体的实例包括水、盐水、林格氏溶液、右旋糖或其他糖溶液、汉克氏溶液和其他生理上平衡的水性盐溶液、磷酸盐缓冲液、碳酸氢盐缓冲液以及Tris缓冲液。另外,该组合物可以包括延长组合物半衰期的化合物。各种杀昆虫制剂也可见于例如美国公开2008/0275115、2008/0242174、2008/0027143、2005/0042245和2004/0127520。
已经认识到,可使用包含编码沉默元件的序列的多核苷酸来转化生物体,以便宿主生物体产生这些组分,并随后将该宿主生物体施用到一种或多种靶标有害生物的环境中。此类宿主生物体包括杆状病毒、细菌等等。以这种方式,可通过合适的载体将编码沉默元件的多核苷酸的组合引入微生物宿主中,并将所述宿主施用到环境中或者施用到植物或动物上。
在将核酸插入细胞的背景下,术语“引入”是指“转染”或“转化”或“转导”,并且包括将核酸并入真核或原核细胞中,在该真核或原核细胞中核酸可以稳定地被并入细胞的基因组(例如,染色体、质粒、质体或线粒体DNA)中,转化为自主复制子,或被瞬时表达(例如经转染的mRNA)。
可选择如下微生物宿主,已知该微生物宿主可占领一种或多种目的作物的“植物圈”(叶面、叶围、根围和/或根面)。选择这些微生物以便能够在具体环境中成功地与野生型微生物竞争,为编码沉默元件的序列供给稳定的维持和表达,并且理想的是,增加对这些组分的保护使其不受环境降解和失活的影响。
此类微生物包括细菌、藻类和真菌。特定的目的是微生物,例如细菌,如假单胞菌属(Pseudomonas)、欧文氏菌属(Erwinia)、沙雷氏菌属(Serratia)、克雷伯氏杆菌属(Klebsiella)、黄单胞菌属(Xanthomonas)、链霉菌属(Streptomyces)、根瘤菌属(Rhizobium)、红假单胞菌属(Rhodopseudomonas)、甲基菌属(Methylius)、农杆菌属(Agrobacterium)、醋酸杆菌属(Acetobacter)、乳酸杆菌属(Lactobacillus)、节细菌属(Arthrobacter)、固氮菌属(Azotobacter)、明串珠菌属(Leuconostoc)和产碱杆菌属(Alcaligenes);真菌,尤其是酵母,如酵母菌属(Saccharomyces)、隐球菌属(Cryptococcus)、克鲁维酵母菌属(Kluyveromyces)、掷孢酵母属(Sporobolomyces)、红酵母(Rhodotorula)和短梗霉属(Aureobasidium)。特定的目的是诸如以下的植物圈细菌物种:丁香假单胞菌(Pseudomonas syrmgae)、荧光假单胞菌(Pseudomonasfluorescens)、粘质沙雷氏菌(Serratia marcescens)、木醋杆菌(Acetobacter xylinum)、农杆菌、球形红假单胞菌(Rhodopseudomonas spheroides)、野油菜黄单胞菌(Xanthomonas campestris)、苜蓿根瘤菌(Rhizobium melioti)、富营养产碱菌(Alcaligenes entrophus)、木质棍状杆菌(Clavibacter xyli)和维涅兰德固氮菌(Azotobacter vinlandir);以及诸如以下的植物圈酵母物种:深红酵母(Rhodotorula rubra)、粘红酵母(R.glutinis)、海滨红酵母(R.marina)、橙黄红酵母(R.aurantiaca)、浅白色隐球酵母(Cryptococcusalbidus)、流散隐球酵母(C.diffluens)、罗伦隐球酵母(C.laurentii)、罗斯酵母(Saccharomycesrosei)、普地酵母(S.pretoriensis)、酿酒酵母(S.cerevisiae)、粉红掷孢酵母(Sporobolomycesrosues)、香气掷孢酵母(S.odorus)、佛地克鲁维酵母(Kluyveromycesveronae)如出芽短梗霉(Aureobasidiumpollulans)。特定的目的是有颜色的微生物。
有多种方式可用来将包含沉默元件的多核苷酸引入处于如下条件下的微生物宿主中,该条件容许这种核苷酸编码序列的稳定维持和表达。例如,可以构建出如下表达盒,该表达盒包括为了该核苷酸构建体的表达与转录和翻译调节信号有效地连接的目的核苷酸构建体,以及与宿主生物体(在此将发生合并)中的序列同源的核苷酸序列和/或在该宿主(在此将发生合并或稳定维持)中具有功能的复制系统。
转录和翻译调节信号包括但不限于启动子、转录起始位点、操纵子、活化子、增强子、其他调节元件、核糖体结合位点、起始密码子、终止信号等。参见,例如美国专利号5,039,523和4,853,331;EP 0480762 A2;Sambrook等人(2000);Molecular Cloning:ALaboratory Manual(3rd edition;Cold Spring Harbor Laboratory Press,Plainview,Ny)[分子克隆:实验室手册(第3版;冷泉港实验室出版社,冷泉港,纽约州)];Davis等人(1980)Advanced Bacterial Genetics(Cold Spring Harbor Laboratory,Cold SpringHarbor,NY)[高级细菌遗传学(冷泉港实验室出版社,冷泉港,纽约州)];以及其中引用的参考文献。
合适的宿主细胞包括原核生物和低等真核生物,如真菌。说明性的原核生物(革兰氏阴性和革兰氏阳性),包括肠杆菌科,如埃希氏杆菌属、欧文氏菌属、志贺氏杆菌、沙门氏菌和变形杆菌;芽孢杆菌科;根瘤科,如根瘤菌;螺菌科,如发光杆菌属、单胞发酵菌属、沙雷菌属、气单孢菌属、弧菌属、脱硫弧菌属、螺旋菌属;乳酸细菌科;假单胞菌科,如假单胞菌属和醋菌属;固氮菌科和硝化杆菌科。真核生物中的真菌,如藻菌和子囊菌纲(包括酵母,如酵母菌属和裂殖酵母属;以及担子菌纲酵母,如红酵母、短梗霉、掷孢酵母等)。
在选择宿主细胞上特定的目的特征可以包括易于将编码序列引入该宿主中、表达系统的可用性、表达效率、宿主中的稳定性以及辅助遗传能力的存在。用作杀有害生物剂微胶囊的目的特征包括保护性性质,如细胞壁厚、色素沉着、以及细胞内包装或包涵体的形成;叶亲和力;无哺乳动物毒性;吸引有害生物摄取;等等。其他考虑因素包括易于配制和处理、经济性、储存稳定性等。
特定的目的宿主微生物包括酵母,例如红酵母属物种(Rhodotorula spp.)、短梗霉属物种(Aureobasidium spp.)、酵母属物种(Saccharomyces spp.)和掷孢酵母属物种(Sporobolomyces spp.),叶面生物体,例如假单胞菌属物种(Pseudomonas spp.)、欧文氏菌属物种(Erwinia spp.)如黄杆菌属物种(Flavobacterium spp.),以及其他此类生物体,包括铜绿假单胞菌(Pseudomonas aerugmosa)、荧光假单胞菌、酿酒酵母(Saccharomycescerevisiae)、苏云金芽孢杆菌(Bacillus thuringiensis)、大肠杆菌(Escherichiacoli)、枯草芽孢杆菌(Bacillussubtilis)等。
本发明所包含的编码沉默元件的序列可以被引入到植物(附生植物)上繁殖的微生物中,以将这些成分传递给潜在的靶标有害生物。附生植物,例如可以是革兰氏阳性或革兰氏阴性细菌。
可以将沉默元件在细菌宿主中发酵,并且将所得到的细菌以与苏云金芽孢杆菌菌株已被用作杀昆虫喷雾剂相同的方式进行加工并用作微生物喷雾剂。任何合适的微生物都可以用于此目的。举例来说,假单胞菌已被用来表达作为包封的蛋白质的苏云金芽孢杆菌内毒素,并且将所得细胞进行加工并将其作为杀昆虫剂进行喷雾(Gaertner等人(1993),Advanced Engineered Pesticides[高级工程杀有害生物剂],编辑L.Kim(马塞尔·德克尔公司(Marcel Decker,Inc.)))。
可替代地,通过将异源基因引入细胞宿主中来产生本发明的组分。异源序列的表达直接或间接地导致沉默元件在细胞内产生。然后可以根据常规技术配制这些组合物,以施用于靶标有害生物所寄宿的环境(例如,土壤、水和植物的叶子)中。参见,例如EPA0192319及其中引用的参考文献。
可以用可接受的载体将经转化的微生物配制成单独的或组合的组合物,该组合物为例如悬浮液、溶液、乳剂、扑粉、可分散颗粒、可湿性粉末、以及乳油、气雾剂、浸渍颗粒、辅剂、可涂覆型糊剂,以及还有如聚合物物质中的包封物。
以上所披露的这类组合物可通过添加以下物质来获得:表面活性剂、惰性载体、防腐剂、保湿剂、取食刺激剂、引诱剂、包囊剂、粘合剂、乳化剂、染料、紫外保护剂、缓冲剂、助流剂或肥料、微量营养物供体或其他能影响植物生长的制剂。包括但不限于除草剂、杀昆虫剂、杀真菌剂、杀细菌剂、杀线虫剂、杀软体动物剂、杀螨剂、植物生长调节剂、收获助剂(harvest aid)和肥料的一种或多种农用化学品可以与制剂或其他组分的本领域通常使用的载体、表面活性剂或佐剂组合,以促进特定靶标有害生物的产品处理和应用。合适的载体和佐剂可以是固体或液体并且对应于制剂技术中通常使用的物质,例如,天然或再生矿物质、溶剂、分散剂、润湿剂、增粘剂、粘合剂或肥料。这些活性成分(即至少一种沉默元件)通常以组合物的形式被施用,并且可以施用于待处理的作物区域、植物或种子上。例如,可以在准备贮藏于粮仓或筒仓等中或者在贮藏于粮仓或筒仓等中的过程中将这些组合物施用于谷物上。可以将这些组合物与其他化合物同时或相继施用。施用含有至少一种沉默元件的活性成分或组合物的方法包括但不限于叶面施用、种子包衣和土壤施用。施用次数和施用速度取决于相应有害生物侵袭的强度。
合适的表面活性剂包括但不限于阴离子化合物,例如羧酸盐,如金属的羧酸盐;长链脂肪酸的羧酸盐;N-酰基肌氨酸盐;磷酸与脂肪醇乙氧基化物的单酯或二酯或这些酯的盐;脂肪醇硫酸盐,如十二烷基硫酸钠、十八烷基硫酸钠或十六烷基硫酸钠;乙氧基化脂肪醇硫酸盐;乙氧基化烷基酚硫酸盐;木质素磺酸盐;石油磺酸盐;烷基芳基磺酸盐,例如烷基苯磺酸盐或低级烷基木素磺化盐,如丁基木素磺化盐;磺化萘-甲醛缩合物的盐;磺化苯酚-甲醛缩合物的盐;更复杂的磺酸盐,例如酰胺磺酸盐,如油酸和N-甲基牛磺酸的磺化缩合产物;或二烷基磺基琥珀酸盐,例如,磺酸钠或二辛基琥珀酸盐。非离子剂包括脂肪酸酯、脂肪醇、脂肪酸酰胺或者经脂肪烷基或烯基取代的酚与环氧乙烷的缩合产物,多元醇醚的脂肪酯(例如脱水山梨糖醇脂肪酸酯),这种酯类与环氧乙烷的缩合产物(例如聚氧乙烯脱水山梨糖醇脂肪酸酯),环氧乙烷和环氧丙烷的嵌段共聚物,炔属乙二醇(例如2,4,7,9-四乙基-5-癸炔-4,7-二醇,或者乙氧基化的炔属乙二醇)。阳离子表面活性剂的实例包括例如脂肪族单胺、二胺或多胺诸如乙酸盐、环烷酸盐或者油酸盐;或含氧胺,如聚氧乙烯烷基胺的氧化胺;通过将羧酸与二胺或多胺进行缩合制备的酰胺连接的胺;或季铵盐。
惰性材料的实例包括但不限于,无机矿物(如高岭土、页硅酸盐、碳酸盐、硫酸盐、磷酸盐)或植物材料(如软木、粉末玉米芯、花生壳、稻壳和核桃壳)。
包含沉默元件的这些组合物可以处于用于直接施用的合适形式或作为主要组合物的浓缩物,该浓缩物在施用前需要用适量的水或其他稀释剂进行稀释。
这些组合物(包括经转化的微生物)可以通过例如以如下方式施用于昆虫有害生物(例如鞘翅目植物有害生物或叶甲属植物有害生物)的环境中:喷雾、雾化、撒粉、散射、涂覆或浇注、引入到土壤中或土壤上、引入到灌溉水中、在有害生物已开始出现时或者在有害生物出现前进行种子处理或普通施用或撒粉作为保护措施。例如,可以将该一种或多种组合物和/或一种或多种经转化的微生物与谷物混合以在储存期间保护谷物。通常重要的是在植物生长的早期阶段获得对有害生物的良好防治,因为这是植物可能受到最严重损害的时候。如果认为必要,这些组合物可方便地含有另一种杀昆虫剂。在本发明的实施例中,在种植时将该一种或多种组合物直接施用于土壤,施用的形式为载体与芽孢杆菌菌株的或者本发明经转化微生物的死细胞的组合物的颗粒形式。另一个实施例是包含惰性载体中的农业化学品(例如除草剂、杀昆虫剂、肥料)与芽孢杆菌菌株的或本发明经转化微生物的死细胞的组合物的颗粒形式。
Ⅶ.植物、植物部分及将序列引入植物中的方法
在一个实施例中,本发明的方法涉及将多核苷酸引入植物中。“引入”旨在表示将该多核苷酸呈送给该植物,以这样的方式使得该序列进入该植物细胞的内部。本发明的方法不取决于用于将序列引入植物中的具体方法,只要多核苷酸或多肽进入该植物的至少一个细胞的内部即可。将多核苷酸引入植物的方法是本领域已知的,该方法包括但不限于稳定转化法、瞬时转化法和病毒介导法。
“稳定转化”旨在表示经引入植物中的核苷酸构建体合并到该植物的基因组中,并且能够被其子代遗传。“瞬时转化”旨在表示将多核苷酸引入该植物中并且不合并到该植物的基因组中,或者将多肽引入植物中。
转化方案连同用于将多肽或多核苷酸序列引入植物中的方案可以取决于被靶向转化的植物或植物细胞的类型(即,单子叶植物或双子叶植物)而变化。用于将多肽和多核苷酸引入植物细胞的适合的方法包括显微注射(Crossway等人(1986)Biotechniques[生物技术]4:320-334)、电穿孔(Riggs等人(1986)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]83:5602-5606)、农杆菌介导的转化(美国专利号5,563,055和美国专利号5,981,840)、直接基因转移(Paszkowski等人(1984)EMBO J.[欧洲分子生物学学会杂志]3:2717-2722)、和弹道粒子加速法(参见例如美国专利号4,945,050;美国专利号5,879,918;美国专利号5,886,244;和5,932,782;Tomes等人(1995),Plant Cell,Tissue,and Organ Culture:Fundamental Methods[植物细胞、组织和器官培养:基础方法],Gamborg和Phillips编辑(施普林格出版公司(Springer-Verlag),柏林);McCabe等人(1988)Biotechnology[生物技术]6:923-926);和Lec1转化法(WO 00/28058)。也参见Weissinger等人(1988)Ann.Rev.Genet.[遗传学年鉴]22:421-477;Sanford等人(1987)Particulate Science andTechnology[微粒科学与技术]5:27-37(洋葱);Christou等人(1988)PlantPhysiol.[植物生理学]87:671-674(大豆);McCabe等人(1988)Bio/Technology[生物学/技术]6:923-926(大豆);Finer和McMullen(1991)InVitro Cell Dev.Biol.[体外细胞与发育生物学]27P:175-182(大豆);Singh等人(1998)Theot.Appl.Genet.[理论与应用遗传学]96:319-324(大豆);Datta等人(1990)Biotechnology[生物技术]8:736-740(稻);Klein等人(1988)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]85:4305-4309(玉米);Klein等人(1988)Biotechnology[生物技术]6:559-563(玉米);美国专利号5,240,855、5,322,783、和5,324,646;Klein等人(1988)Plant Physiol.[植物生理学]91:440-444(玉米);Fromm等人(1990)Biotechnology[生物技术]8:833-839(玉米);Hooykaas-Van Slogteren等人(1984)Nature[自然](伦敦)311:763-764;美国专利号5,736,369(谷类);Bytebier等人(1987)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]84:5345-5349(百合科);De Wet等人(1985)在The ExperimentalManipulation of Ovule Tissues[卵巢组织的实验操作]中Chapman等人编辑(纽约朗文出版社(Longman,New York))第197-209页(花粉);Kaeppler等人(1990)Plant Cell Reports[植物细胞报告]9:415-418和Kaeppler等人(1992)Theor.App l.Genet.[理论与应用遗传学]84:560-566(须介导的转化);D′Halluin等人(1992)Plant Cell[植物细胞]4:1495-1505(电穿孔);Li等人(1993)Plant CellReports[植物细胞报告]12:250-255以及Christou和Ford(1995)Annalso of Botany[植物学年鉴]75:407-413(稻);Osjoda等人(1996)Nature Biotechnology[自然生物技术]14:745-750(maize via Agrobacterium tumefaciens[经由根癌农杆菌的玉米])。
在某些实施例中,可以使用各种瞬时转化法向植物提供本文披露的沉默元件。这种瞬时转化法包括但不限于将蛋白质或者其变体或片段直接引入植物中或将转录物引入植物中。此类方法包括例如显微注射或粒子轰击。参见例如Crossway等人(1986)MolGen.Genet.[分子遗传学和基因组学]202:179-185;Nomura等人(1986)Plant Sci.[植物科学]44:53-58;Hepler等人(1994)Proc.Natl.Acad.Sci.[国家科学院院刊]91:2176-2180和Hush等人(1994)The Journal of Cell Science[细胞科学杂志]107:775-784,以上所有文献都通过引用并入本文。可替代地,可以使用本领域已知的技术将多核苷酸瞬时转化到植物中。此类技术包括病毒载体系统,和以阻止DNA后续释放的方式使多核苷酸沉淀。此类方法包括使用涂覆有聚乙亚胺(PEI;西格玛公司#P3143)的颗粒。
在其他实施例中,可以通过使植物与病毒或病毒核酸接触将本文披露的多核苷酸引入植物中。通常,此类方法涉及将本发明的核苷酸构建体并入病毒DNA或RNA分子内。此外,已经认识到,启动子还可以涵盖用于通过病毒RNA聚合酶转录的启动子。涉及病毒DNA或RNA分子、用于将多核苷酸引入植物并表达其中所编码的蛋白质的方法是本领域已知的。参见例如,美国专利号5,889,191、5,889,190、5,866,785、5,589,367、5,316,931和Porta等人(1996)Molecular Biotechnology[分子生物技术]5:209-221。
用于在植物基因组的特定位置定向插入多核苷酸的方法是本领域已知的。在一个实施例中,利用位点特异性重组系统实现多核苷酸在所期望的基因组位置的插入。参见例如WO 99/25821、WO 99/25854、WO 99/25840、WO 99/25855和WO 99/25853。简而言之,本文披露的多核苷酸可以包含在两侧为两个非引起重组的重组位点的转运盒中。将转移盒引入使如下靶标位点稳定地并入其基因组中的植物中,该靶标位点的侧翼为两个与该转移盒的这些位点相对应的非引起重组的重组位点。提供适当的重组酶,并将该转移盒整合到靶标位点。目的多核苷酸因而被并入到植物基因组中的特定染色体位置上。
已经转化的细胞可以按照常规方法生长为植物。参见例如,McCormick等人(1986)Plant Cell Reports[植物细胞报告]5:81-84。然后可以培育这些植株,并用相同的经转化的株系或者不同的株系进行授粉,并鉴定出具有所希望的表型特征的组成型表达的所得后代。可以培育两代或更多代植株,确保所希望的表型特征的表达得到稳定保持和遗传,并且然后收获种子,确保已实现所希望的表型特征的表达。以这种方式,本文所述的组合物和方法提供了如下经转化的种子(也称为“转基因种子”),该种子本文披露的多核苷酸(例如表达盒)稳定地并入其基因组中。
如本文所使用的,术语植物包括植物细胞、植物原生质体、可再生植物的植物细胞组织培养物、植物愈伤组织、植物块和在植物或植物部分(如胚胎、花粉、胚珠、种子、叶、花、枝、果、核、穗、穗轴、壳、茎、根、根尖、花药等)中完好的植物细胞。谷物意指由商业种植者出于栽培或繁殖物种之外的目的所生产的成熟种子。这些再生植物的后代、变体和突变体也包括在本发明的范围内,条件是这些部分包含经引入的多核苷酸。
本文描述的组合物和方法可用于转化任何植物物种,包括但不限于单子叶植物和双子叶植物。目的植物物种的实例包括但不限于玉米(玉蜀黍),芸苔属物种(例如,甘蓝型油菜、芜菁、芥菜)(特别是可用作种子油来源的那些芸苔属物种),苜蓿(紫花苜蓿(Medicago sativa)),稻(rice,Oryza sativa),黑麦(rye,Secale cereale),高粱(甜高粱(Sorghum bicolor),高粱(Sorghum vulgare)),粟(例如,珍珠粟(御谷(Pennisetumglaucum)),黍(粟米(Panicum miliaceum)),粟(谷子(Setaria italica)),穇子(龙爪稷(Eleusine coracana))),向日葵(sunflower,Helianthus annuus),红花(safflower,Carthamus tinctorius),小麦(wheat,Triticum aestivum),大豆(soybean,Glycinemax),烟草(tobacco,Nicotiana tabacum),马铃薯(potato,Solanum tuberosum),花生(peanut,Arachis hypogaea),棉花(海岛棉(Gossypium barbadense)、陆地棉(Gossypiumhirsutum)),甘薯(番著(Ipomoea batatas)),木薯(cassava,Manihot esculenta),咖啡(咖啡属物种(Coffea spp.)),椰子(coconut,Cocos nucifera),菠萝(pineapple,Ananascomosus),柑橘树(柑橘属物种(Citrus spp.)),可可(cocoa,Theobroma cacao),茶树(tea,Camellia sinensis),香蕉(芭蕉属物种(Musa spp.)),鳄梨(avocado,Perseaamericana),无花果(fig或(Ficus casica)),番石榴(guava,Psidium guajava),芒果(mango,Mangifera indica),橄榄(olive,Olea europaea),木瓜(番木瓜(Caricapapaya)),腰果(cashew,Anacardium occidentale),澳洲坚果(macadamia,Macadamiaintegrifolia),巴旦杏(almond,Prunus amygdalus),甜菜(sugar beets,Betavulgaris),甘蔗(甘蔗属物种(Saccharum spp.)),燕麦,大麦,蔬菜,观赏植物和针叶树。
蔬菜包括番茄(tomatoes,Lycopersicon esculentum)、莴苣(例如,莴苣(Lactucasativa))、青豆(菜豆(Phaseolus vulgaris))、利马豆(lima bean,Phaseolus limensis)、豌豆(香豌豆属物种(Lathyrus spp.))和黄瓜属(Cucumis)的成员诸如黄瓜(cucumber,C.sativus)、香瓜(cantaloupe,C.cantalupensis)和甜瓜(musk melon,C.melo)。观赏植物包括杜鹃(杜鹃花属物种(Rhododendron spp.))、绣球花(hydrangea,Macrophyllahydrangea)、木槿(hibiscus,Hibiscus rosasanensis)、玫瑰(蔷薇属物种(Rosa spp.))、郁金香(郁金香属物种(Tulipa spp.))、水仙(水仙属物种(Narcissus spp.))、矮牵牛(pemnias,Pemnia hybrida)、康乃馨(carnation,Dianthus caryophyllus)、一品红(poinsettia,Euphorbia pulcherrima)和菊花。
可以用于实践本文所述组合物和方法的针叶树包括(例如)松树诸如火炬松(loblolly pine,Pinus taeda)、湿地松(slash pine,Pinus elliotii)、西黄松(ponderosa pine,Pinus ponderosa)、黑松(lodgepole pine,Pinus contorta)和辐射松(Monterey pine,Pinus radiata);花旗松(Douglas-fir,Pseudotsuga menziesii);西方铁杉(Western hemlock,Tsuga canadensis);北美云杉(白云杉(Picea glauca));红杉(北差红杉(Sequoia sempervirens));枞树(truefirs)诸如银杉(胶冷杉(Abies amabilis))和胶枞(香脂冷杉(Abies balsamea));以及雪松,如西方红雪松(北美乔柏(Thujaplicata))和阿拉斯加黄雪松(黄扁柏(Chamaecyparis nootkatensis))。在某些实施例中,本文所述组合物和方法可以与植物一起使用,例如作物植物(如玉米、苜蓿、向日葵、芸苔、大豆、棉花、红花、花生、高粱、小麦、粟、烟草等)。在其他实施例中,玉米和大豆植物以及甘蔗植物是最佳的,并且在仍然其他的实施例中,玉米植物是最佳的。
其他目的植物包括提供目的种子的谷物类植物、油料种子植物和豆科植物。目的种子包括谷物种子,如玉米、小麦、大麦、稻、高粱、黑麦等的种子。油料种子植物包括棉花、大豆、红花、向日葵、芸苔、玉米、苜蓿、棕榈、椰子等。豆科植物包括豆类和豌豆。豆类包括瓜尔豆、槐豆、胡芦巴、大豆、四季豆、豇豆、绿豆、利马豆、蚕豆、小扁豆、鹰嘴豆等。
VIII.转基因植物中性状的堆叠
转基因植物可以包含大量的如本文所披露的如SEQ ID NO.:1-53或107-254中所示的一个或多个靶标多核苷酸、或者其变体或片段、或者其互补序列,以及一种或多种另外的多核苷酸,该多核苷酸导致多个多肽序列的产生或抑制。包含堆叠的多核苷酸序列的转基因植物可以通过传统育种方法或基因工程方法中的一种或两种获得。这些方法包括但不限于,育种各自包含目的多核苷酸的单个品系、如本文所披露的用随后的基因转化包含如下表达构建体的转基因植物,该表达构建体包含如SEQ ID NO.:1-53或107-254中所示的各种靶标多核苷酸,或者编码涉及这种靶标序列变体或其片段、或其互补序列的沉默元件,和将基因共转化入单个植物细胞中。如本文所使用的,术语“堆叠”包括使多个性状存在于相同植物中(即,两个性状都并入核基因组中、一个性状并入核基因组中并且另一个性状并入质体的基因组中、或者两种性状都并入质体的基因组中)。在一个非限制性实例中,“堆叠的性状”包括如下分子堆叠物,在该分子堆叠物中,这些序列在物理上彼此相邻。本文所使用的性状是指从特定序列或序列组衍生的表型。可以使用包含多个多核苷酸或分别携带于多个载体上的多核苷酸的单一转化载体进行多核苷酸的共转化。如果通过对该植物进行基因转化来堆叠这些序列,则可以在任何时间和以任何顺序组合目的这些多核苷酸序列。可以用共转化方案将这些性状与转化盒的任何组合所提供的目的多核苷酸一起引入。例如,如果要引入两个序列,则这两个序列可以包含在单独的转化盒(反式)中或包含在相同的转化盒(顺式)中。这些序列的表达可以由相同的启动子或不同的启动子驱动。进一步认识到,使用位点特异性重组系统可以将多核苷酸序列堆叠在所期望的基因组位置上。参见,例如WO1999/25821、WO 1999/25854、WO 1999/25840、WO 1999/25855和WO 1999/25853。
在一些实施例中,如本文所披露的,单独的或与一种或多种另外的昆虫抗性性状相堆叠的如SEQ ID NO.:1-53或107-254中所示的各种靶标多核苷酸、涉及这类靶标序列的沉默元件、以及其变体或片段、或其互补序列,可以与一种或多种另外的投入性状(例如,除草剂抗性、真菌抗性、病毒抗性、胁迫耐受性、抗病性、雄性不育性、茎秆强度等)或产出性状(例如,增加的产量、经修饰的淀粉、改进的油特性、平衡的氨基酸、高赖氨酸或甲硫氨酸、增加的消化性、改善的纤维品质、抗旱性等)相堆叠。因此,多核苷酸实施例可用于提供具有灵活地且成本有效地防治任何数量的农艺有害生物的能力的经改进的作物品质的完整农艺学方案。
可用于堆叠的转基因包括但不限于下文所述的那些转基因。
i.赋予昆虫抗性或抗病性的转基因
(A)植物抗病性基因通常通过植物中抗病性基因(R)的产物与病原体中相应的无毒性(Avr)基因的产物之间的特异性相互作用来活化植物防御。可以用经克隆的抗性基因来转化植物品种,以设计对特定病原菌株具有抗性的植物。参见,例如Jones等人(1994)Science[科学]266:789(cloning of the tomato Cf-9genefor resistance toCladosporium fulvum[克隆番茄Cf-9基因以抵抗番茄叶霉病菌]);Martin等人(1993)Science[科学]262∶1432(tomato Pto genefor resistance to Pseudomonas syringaepv.tomato encodes a protein kinase[用于抵抗丁香假单胞菌番茄致病变种的番茄Pto基因编码蛋白激酶]);Mindrinos等人(1994)Cell[细胞]78∶1089(ArabidopsisRSP2genefor resistance to Pseudomonas syringae[拟南芥RSP2基因用于对抗丁香假单胞菌]),McDowell和Woffenden(2003)Trends Biotechnol.[生物科技趋势]21(4):178-83以及Toyoda等人(2002)Transgenic Res.[转基因研究]11(6):567-82。与野生型植物相比,具有抗病性的植物对病原体更具抗性。
(B)编码苏云金芽孢杆菌蛋白质的基因,其衍生物或其上建模的合成多肽。参见,例如,Geiser等人,(1986)Gene[基因]48∶109,其披露了Bt 6-内毒素基因的克隆和核苷酸序列。此外,编码6-内毒素基因的DNA分子可购自美国典型培养物保藏中心(罗克维尔(Rockville),马里兰州),例如在登录号40098、67136、31995和31998下。经遗传工程化的苏云金芽孢杆菌转基因的其他非限制性实例在以下专利和专利申请中给出,并且特此通过引用并入本文中:美国专利号5,188,960;5,689,052;5,880,275;5,986,177;6,023,013、6,060,594、6,063,597、6,077,824、6,620,988、6,642,030、6,713,259、6,893,826、7,105,332;7,179,965、7,208,474;7,227,056、7,288,643、7,323,556、7,329,736、7,449,552、7,468,278、7,510,878、7,521,235、7,544,862、7,605,304、7,696,412、7,629,504、7,705,216、7,772,465、7,790,846、7,858,849和WO 1991/14778;WO 1999/31248;WO 2001/12731;WO 1999/24581和WO 1997/40162。
也可以堆叠编码杀有害生物蛋白的基因,该基因包括但不限于:来自假单胞菌属的杀昆虫蛋白,如PSEEN3174(Monalysin,(2011)PLoS Pathogens[PLoS病原体],7∶1-13),来自假单胞菌蛋白菌菌株CHA0和Pf-5(此前为荧光假单胞菌(fluorescens))(Pechy-Tarr,(2008)Environmental Microbiology[环境微生物学]10∶2368-2386:基因库登录号EU400157);来自台湾假单胞菌(Pseudomonas Taiwanensis)(Liu等人,(2010)J.Agric.Food Chem.[农业食品化学学报]58∶12343-12349)和来自假产碱假单胞菌(Pseudomonas pseudoalcaligenes)(Zhang等人,(2009)Annals of Microbiology[微生物学年报]59∶45-50和Li等人,(2007)Plant Cell Tiss.Organ Cult.[植物细胞组织和器官培养]89∶159-168);来自发光杆菌属和致病杆菌属的杀昆虫蛋白(Hinchliffe等人,(2010)The Open Toxinology Journal[开放性毒理学杂志]3∶101-118和Morgan等人,(2001)Applied and Envir.Micro.[应用与环境微生物学]67∶2062-2069),美国专利号6,048,838和美国专利号6,379,946;美国专利公开号US 20140007292的PIP-1多肽;美国专利公开号US 20140033361的AfIP-1A和/或AfIP-1B多肽;美国专利公开US 20140274885和US20160040184的PHI-4多肽;PCT公开号WO 2015/023846的PIP-47多肽、PCT公开号WO 2015/038734的PIP-72多肽;PCT公开号WO 2015/120270的PtIP-50多肽和PtIP-65多肽;PCT公开号WO 2015/120276的PtIP-83多肽;PCT序列号PCT/US 15/55502的PtIP-96多肽;以及δ-内毒素,包括但不限于Cry1、Cry2、Cry3、Cry4、Cry5、Cry6、Cry7、Cry8、Cry9、Cry10、Cry11、Cry12、Cry13、Cry14、Cry15、Cry16、Cry17、Cry18、Cry19、Cry20、Cry21、Cry22、Cry23、Cry24、Cry25、Cry26、Cry27、Cry28、Cry29、Cry30、Cry31、Cry32、Cry33、Cry34、Cry35、Cry36、Cry37、Cry38、Cry39、Cry40、Cry41、Cry42、Cry43、Cry44、Cry45、Cry46、Cry47、Cry49、Cry51和Cry55类的δ-内毒素基因和苏云金芽孢杆菌溶细胞Cyt1和Cyt2基因。苏云金芽孢杆菌杀昆虫蛋白的这些类别的成员包括但不限于Cry1Aa1(登录号AAA22353);Cry1Aa2(登录号AAA22552);Cry1Aa3(登录号BAA00257);Cry1Aa4(登录号CAA31886);Cry1Aa5(登录号BAA04468);Cry1Aa6(登录号AAA86265);Cry1Aa7(登录号AAD46139);Cry1Aa8(登录号126149);Cry1Aa9(登录号BAA77213);Cry1Aa10(登录号AAD55382);Cry1Aall(登录号CAA70856);Cry1Aa12(登录号AAP80146);Cry1Aa13(登录号AAM44305);Cry1Aa14(登录号AAP40639);Cry1Aa15(登录号AAY66993);Cry1Aa16(登录号HQ439776);Cry1Aa17(登录号HQ439788);Cry1Aa18(登录号HQ439790);Cry1Aa19(登录号HQ685121);Cry1Aa20(登录号JF340156);Cry1Aa21(登录号JN651496);Cry1Aa22(登录号KC158223);Cry1Ab1(登录号AAA22330);Cry1Ab2(登录号AAA22613);Cry1Ab3(登录号AAA22561);Cry1Ab4(登录号BAA00071);Cry1Ab5(登录号CAA28405);Cry1Ab6(登录号AAA22420);Cry1Ab7(登录号CAA31620);Cry1Ab8(登录号AAA22551);Cry1Ab9(登录号CAA38701);Cry1Ab10(登录号A29125);Cry1Ab11(登录号I12419);Cry1Ab12(登录号AAC64003);Cry1Ab13(登录号AAN76494);Cry1Ab14(登录号AAG16877);Cry1Ab15(登录号AAO13302);Cry1Ab16(登录号AAK55546);Cry1Ab17(登录号AAT46415);Cry1Ab18(登录号AAQ88259);Cry1Ab19(登录号AAW31761);Cry1Ab20(登录号ABB72460);Cry1Ab21(登录号ABS18384);Cry1Ab22(登录号ABW87320);Cry1Ab23(登录号HQ439777);Cry1Ab24(登录号HQ439778);Cry1Ab25(登录号HQ685122);Cry1Ab26(登录号HQ847729);Cry1Ab27(登录号JN135249);Cry1Ab28(登录号JN135250);Cry1Ab29(登录号JN135251);Cry1Ab30(登录号JN135252);Cry1Ab31(登录号JN135253);Cry1Ab32(登录号JN135254);Cry1Ab33(登录号AAS93798);Cry1Ab34(登录号KC156668);Cry1Ab样(登录号AAK14336);Cry1Ab样(登录号AAK14337);Cry1Ab样(登录号AAK14338);Cry1Ab样(登录号ABG88858);Cry1Ac1(登录号AAA22331);Cry1Ac2(登录号AAA22338);Cry1Ac3(登录号CAA38098);Cry1Ac4(登录号AAA73077);Cry1Ac5(登录号AAA22339);Cry1Ac6(登录号AAA86266);Cry1Ac7(登录号AAB46989);Cry1Ac8(登录号AAC44841);Cry1Ac9(登录号AAB49768);Cry1Ac10(登录号CAA05505);Cry1Ac11(登录号CAA10270);Cry1Ac12(登录号I12418);Cry1Ac13(登录号AAD38701);Cry1Ac14(登录号AAQ06607);Cry1Ac15(登录号AAN07788);Cry1Ac16(登录号AAU87037);Cry1Ac17(登录号AAX18704);Cry1Ac18(登录号AAY88347);Cry1Ac19(登录号ABD37053);Cry1Ac20(登录号ABB89046);Cry1Ac21(登录号AAY66992);Cry1Ac22(登录号ABZ01836);Cry1Ac23(登录号CAQ30431);Cry1Ac24(登录号ABL01535);Cry1Ac25(登录号FJ513324);Cry1Ac26(登录号FJ617446);Cry1Ac27(登录号FJ617447);Cry1Ac28(登录号ACM90319);Cry1Ac29(登录号DQ438941);Cry1Ac30(登录号GQ227507);Cry1Ac31(登录号GU446674);Cry1Ac32(登录号HM061081);Cry1Ac33(登录号GQ866913);Cry1Ac34(登录号HQ230364);Cry1Ac35(登录号JF340157);Cry1Ac36(登录号JN387137);Cry1Ac37(登录号JQ317685);Cry1Ad1(登录号AAA22340);Cry1Ad2(登录号CAA01880);Cry1Ae1(登录号AAA22410);Cry1Af1(登录号AAB82749);Cry1Ag1(登录号AAD46137);Cry1Ah1(登录号AAQ14326);Cry1Ah2(登录号ABB76664);Cry1Ah3(登录号HQ439779);Cry1Ai1(登录号AAO39719);Cry1Ai2(登录号HQ439780);Cry1A样(登录号AAK14339);Cry1Ba1(登录号CAA29898);Cry1Ba2(登录号CAA65003);Cry1Ba3(登录号AAK63251);Cry1Ba4(登录号AAK51084);Cry1Ba5(登录号ABO20894);Cry1Ba6(登录号ABL60921);Cry1Ba7(登录号HQ439781);Cry1Bb1(登录号AAA22344);Cry1Bb2(登录号HQ439782);Cry1Bc1(登录号CAA86568);Cry1Bd1(登录号AAD10292);Cry1Bd2(登录号AAM93496);Cry1Be1(登录号AAC32850);Cry1Be2(登录号AAQ52387);Cry1Be3(登录号ACV96720);Cry1Be4(登录号HM070026);Cry1Bf1(登录号CAC50778);Cry1Bf2(登录号AAQ52380);Cry1Bg1(登录号AAO39720);Cry1Bh1(登录号HQ589331);Cry1Bi1(登录号KC156700);Cry1Ca1(登录号CAA30396);Cry1Ca2(登录号CAA31951);Cry1Ca3(登录号AAA22343);Cry1Ca4(登录号CAA01886);Cry1Ca5(登录号CAA65457);Cry1Ca6[1](登录号AAF37224);Cry1Ca7(登录号AAG50438);Cry1Ca8(登录号AAM00264);Cry1Ca9(登录号AAL79362);Cry1Ca10(登录号AAN16462);Cry1Ca11(登录号AAX53094);Cry1Ca12(登录号HM070027);Cry1Ca13(登录号HQ412621);Cry1Ca14(登录号JN651493);Cry1Cb1(登录号M97880);Cry1Cb2(登录号AAG35409);Cry1Cb3(登录号ACD50894);Cry1Cb样(登录号AAX63901);Cry1Da1(登录号CAA38099);Cry1Da2(登录号I76415);Cry1Da3(登录号HQ439784);Cry1Db1(登录号CAA80234);Cry1Db2(登录号AAK48937);Cry1Dc1(登录号ABK35074);Cry1Ea1(登录号CAA37933);Cry1Ea2(登录号CAA39609);Cry1Ea3(登录号AAA22345);Cry1Ea4(登录号AAD04732);Cry1Ea5(登录号A15535);Cry1Ea6(登录号AAL50330);Cry1Ea7(登录号AAW72936);Cry1Ea8(登录号ABX11258);Cry1Ea9(登录号HQ439785);Cry1Ea10(登录号ADR00398);Cry1Ea11(登录号JQ652456);Cry1Eb1(登录号AAA22346);Cry1Fa1(登录号AAA22348);Cry1Fa2(登录号AAA22347);Cry1Fa3(登录号HM070028);Cry1Fa4(登录号HM439638);Cry1Fb1(登录号CAA80235);Cry1Fb2(登录号BAA25298);Cry1Fb3(登录号AAF21767);Cry1Fb4(登录号AAC10641);Cry1Fb5(登录号AAO13295);Cry1Fb6(登录号ACD50892);Cry1Fb7(登录号ACD50893);Cry1Ga1(登录号CAA80233);Cry1Ga2(登录号CAA70506);Cry1Gb1(登录号AAD10291);Cry1Gb2(登录号AAO13756);Cry1Gc1(登录号AAQ52381);Cry1Ha1(登录号CAA80236);Cry1Hb1(登录号AAA79694);Cry1Hb2(登录号HQ439786);Cry1H样(登录号AAF01213);Cry1Ia1(登录号CAA44633);Cry1Ia2(登录号AAA22354);Cry1Ia3(登录号AAC36999);Cry1Ia4(登录号AAB00958);Cry1Ia5(登录号CAA70124);Cry1Ia6(登录号AAC26910);Cry1Ia7(登录号AAM73516);Cry1Ia8(登录号AAK66742);Cry1Ia9(登录号AAQ08616);Cry1Ia10(登录号AAP86782);Cry1Ia11(登录号CAC85964);Cry1Ia12(登录号AAV53390);Cry1Ia13(登录号ABF83202);Cry1Ia14(登录号ACG63871);Cry1Ia15(登录号FJ617445);Cry1Ia16(登录号FJ617448);Cry1Ia17(登录号GU989199);Cry1Ia18(登录号ADK23801);Cry1Ia19(登录号HQ439787);Cry1Ia20(登录号JQ228426);Cry1Ia21(登录号JQ228424);Cry1Ia22(登录号JQ228427);Cry1Ia23(登录号JQ228428);Cry1Ia24(登录号JQ228429);Cry1Ia25(登录号JQ228430);Cry1Ia26(登录号JQ228431);Cry1Ia27(登录号JQ228432);Cry1Ia28(登录号JQ228433);Cry1Ia29(登录号JQ228434);Cry1Ia30(登录号JQ317686);Cry1Ia31(登录号JX944038);Cry1Ia32(登录号JX944039);Cry1Ia33(登录号JX944040);Cry1Ib1(登录号AAA82114);Cry1Ib2(登录号ABW88019);Cry1Ib3(登录号ACD75515);Cry1Ib4(登录号HM051227);Cry1Ib5(登录号HM070028);Cry1Ib6(登录号ADK38579);Cry1Ib7(登录号JN571740);Cry1Ib8(登录号JN675714);Cry1Ib9(登录号JN675715);Cry1Ib10(登录号JN675716);Cry1Ib11(登录号JQ228423);Cry1Ic1(登录号AAC62933);Cry1Ic2(登录号AAE71691);Cry1Id1(登录号AAD44366);Cry1Id2(登录号JQ228422);Cry1Ie1(登录号AAG43526);Cry1Ie2(登录号HM439636);Cry1Ie3(登录号KC156647);Cry1Ie4(登录号KC156681);Cry1If1(登录号AAQ52382);Cry1Ig1(登录号KC156701);Cry1I样(登录号AAC31094);Cry1I样(登录号ABG88859);Cry1Jal(登录号AAA22341);Cry1Ja2(登录号HM070030);Cry1Ja3(登录号JQ228425);Cry1Jb1(登录号AAA98959);Cry1Jc1(登录号AAC31092);Cry1Jc2(登录号AAQ52372);Cry1Jd1(登录号CAC50779);Cry1Ka1(登录号AAB00376);Cry1Ka2(登录号HQ439783);Cry1La1(登录号AAS60191);Cry1La2(登录号HM070031);Cry1Ma1(登录号FJ884067);Cry1Ma2(登录号KC156659);Cry1Nal(登录号KC156648);Cry1Nb1(登录号KC156678);Cry1样(登录号AAC31091);Cry2Aa1(登录号AAA22335);Cry2Aa2(登录号AAA83516);Cry2Aa3(登录号D86064);Cry2Aa4(登录号AAC04867);Cry2Aa5(登录号CAA10671);Cry2Aa6(登录号CAA10672);Cry2Aa7(登录号CAA10670);Cry2Aa8(登录号AAO13734);Cry2Aa9(登录号AAO13750);Cry2Aa10(登录号AAQ04263);Cry2Aa11(登录号AAQ52384);Cry2Aa12(登录号ABI83671);Cry2Aa13(登录号ABL01536);Cry2Aa14(登录号ACF04939);Cry2Aa15(登录号JN426947);Cry2Ab1(登录号AAA22342);Cry2Ab2(登录号CAA39075);Cry2Ab3(登录号AAG36762);Cry2Ab4(登录号AAO13296);Cry2Ab5(登录号AAQ04609);Cry2Ab6(登录号AAP59457);Cry2Ab7(登录号AAZ66347);Cry2Ab8(登录号ABC95996);Cry2Ab9(登录号ABC74968);Cry2Ab10(登录号EF157306);Cry2Ab11(登录号CAM84575);Cry2Ab12(登录号ABM21764);Cry2Ab13(登录号ACG76120);Cry2Ab14(登录号ACG76121);Cry2Ab15(登录号HM037126);Cry2Ab16(登录号GQ866914);Cry2Ab17(登录号HQ439789);Cry2Ab18(登录号JN135255);Cry2Ab19(登录号JN135256);Cry2Ab20(登录号JN135257);Cry2Ab21(登录号JN135258);Cry2Ab22(登录号JN135259);Cry2Ab23(登录号JN135260);Cry2Ab24(登录号JN135261);Cry2Ab25(登录号JN415485);Cry2Ab26(登录号JN426946);Cry2Ab27(登录号JN415764);Cry2Ab28(登录号JN651494);Cry2Ac1(登录号CAA40536);Cry2Ac2(登录号AAG35410);Cry2Ac3(登录号AAQ52385);Cry2Ac4(登录号ABC95997);Cry2Ac5(登录号ABC74969);Cry2Ac6(登录号ABC74793);Cry2Ac7(登录号CAL18690);Cry2Ac8(登录号CAM09325);Cry2Ac9(登录号CAM09326);Cry2Ac10(登录号ABN15104);Cry2Ac11(登录号CAM83895);Cry2Ac12(登录号CAM83896);Cry2Ad1(登录号AAF09583);Cry2Ad2(登录号ABC86927);Cry2Ad3(登录号CAK29504);Cry2Ad4(登录号CAM32331);Cry2Ad5(登录号CAO78739);Cry2Ae1(登录号AAQ52362);Cry2Af1(登录号ABO30519);Cry2Af2(登录号GQ866915);Cry2Ag1(登录号ACH91610);Cry2Ah1(登录号EU939453);Cry2Ah2(登录号ACL80665);Cry2Ah3(登录号GU073380);Cry2Ah4(登录号KC156702);Cry2Ai1(登录号FJ788388);Cry2Aj(登录号);Cry2Ak1(登录号KC156660);Cry2Ba1(登录号KC156658);Cry3Aa1(登录号AAA22336);Cry3Aa2(登录号AAA22541);Cry3Aa3(登录号CAA68482);Cry3Aa4(登录号AAA22542);Cry3Aa5(登录号AAA50255);Cry3Aa6(登录号AAC43266);Cry3Aa7(登录号CAB41411);Cry3Aa8(登录号AAS79487);Cry3Aa9(登录号AAW05659);Cry3Aa10(登录号AAU29411);Cry3Aa1l(登录号AAW82872);Cry3Aa12(登录号ABY49136);Cry3Ba1(登录号CAA34983);Cry3Ba2(登录号CAA00645);Cry3Ba3(登录号JQ397327);Cry3Bb1(登录号AAA22334);Cry3Bb2(登录号AAA74198);Cry3Bb3(登录号I15475);Cry3Ca1(登录号CAA42469);Cry4Aa1(登录号CAA68485);Cry4Aa2(登录号BAA00179);Cry4Aa3(登录号CAD30148);Cry4Aa4(登录号AFB18317);Cry4A样(登录号AAY96321);Cry4Ba1(登录号CAA30312);Cry4Ba2(登录号CAA30114);Cry4Ba3(登录号AAA22337);Cry4Ba4(登录号BAA00178);Cry4Ba5(登录号CAD30095);Cry4Ba样(登录号ABC47686);Cry4Ca1(登录号EU646202);Cry4Cb1(登录号FJ403208);Cry4Cb2(登录号FJ597622);Cry4Cc1(登录号FJ403207);Cry5Aa1(登录号AAA67694);Cry5Ab1(登录号AAA67693);Cry5Ac1(登录号I34543);Cry5Ad1(登录号ABQ82087);Cry5Ba1(登录号AAA68598);Cry5Ba2(登录号ABW88931);Cry5Ba3(登录号AFJ04417);Cry5Ca1(登录号HM461869);Cry5Ca2(登录号ZP_04123426);Cry5Da1(登录号HM461870);Cry5Da2(登录号ZP_04123980);Cry5Ea1(登录号HM485580);Cry5Ea2(登录号ZP_04124038);Cry6Aa1(登录号AAA22357);Cry6Aa2(登录号AAM46849);Cry6Aa3(登录号ABH03377);Cry6Ba1(登录号AAA22358);Cry7Aa1(登录号AAA22351);Cry7Ab1(登录号AAA21120);Cry7Ab2(登录号AAA21121);Cry7Ab3(登录号ABX24522);Cry7Ab4(登录号EU380678);Cry7Ab5(登录号ABX79555);Cry7Ab6(登录号ACI44005);Cry7Ab7(登录号ADB89216);Cry7Ab8(登录号GU145299);Cry7Ab9(登录号ADD92572);Cry7Ba1(登录号ABB70817);Cry7Bb1(登录号KC156653);Cry7Ca1(登录号ABR67863);Cry7Cb1(登录号KC156698);Cry7Da1(登录号ACQ99547);Cry7Da2(登录号HM572236);Cry7Da3(登录号KC156679);Cry7Ea1(登录号HM035086);Cry7Ea2(登录号HM132124);Cry7Ea3(登录号EEM19403);Cry7Fa1(登录号HM035088);Cry7Fa2(登录号EEM19090);Cry7Fb1(登录号HM572235);Cry7Fb2(登录号KC156682);Cry7Ga1(登录号HM572237);Cry7Ga2(登录号KC156669);Cry7Gb1(登录号KC156650);Cry7Gc1(登录号KC156654);Cry7Gd1(登录号KC156697);Cry7Ha1(登录号KC156651);Cry7Ia1(登录号KC156665);Cry7Ja1(登录号KC156671);Cry7Ka1(登录号KC156680);Cry7Kb1(登录号BAM99306);Cry7La1(登录号BAM99307);Cry8Aa1(登录号AAA21117);Cry8Ab1(登录号EU044830);Cry8Ac1(登录号KC156662);Cry8Ad1(登录号KC156684);Cry8Ba1(登录号AAA21118);Cry8Bb1(登录号CAD57542);Cry8Bc1(登录号CAD57543);Cry8Ca1(登录号AAA21119);Cry8Ca2(登录号AAR98783);Cry8Ca3(登录号EU625349);Cry8Ca4(登录号ADB54826);Cry8Da1(登录号BAC07226);Cry8Da2(登录号BD133574);Cry8Da3(登录号BD133575);Cry8Db1(登录号BAF93483);Cry8Ea1(登录号AAQ73470);Cry8Ea2(登录号EU047597);Cry8Ea3(登录号KC855216);Cry8Fa1(登录号AAT48690);Cry8Fa2(登录号HQ174208);Cry8Fa3(登录号AFH78109);Cry8Ga1(登录号AAT46073);Cry8Ga2(登录号ABC42043);Cry8Ga3(登录号FJ198072);Cry8Ha1(登录号AAW81032);Cry8Ia1(登录号EU381044);Cry8Ia2(登录号GU073381);Cry8Ia3(登录号HM044664);Cry8Ia4(登录号KC156674);Cry8Ib1(登录号GU325772);Cry8Ib2(登录号KC156677);Cry8Ja1(登录号EU625348);Cry8Ka1(登录号FJ422558);Cry8Ka2(登录号ACN87262);Cry8Kb1(登录号HM123758);Cry8Kb2(登录号KC156675);Cry8La1(登录号GU325771);Cry8Ma1(登录号HM044665);Cry8Ma2(登录号EEM86551);Cry8Ma3(登录号HM210574);Cry8Na1(登录号HM640939);Cry8Pa1(登录号HQ388415);Cry8Qa1(登录号HQ441166);Cry8Qa2(登录号KC152468);Cry8Ra1(登录号AFP87548);Cry8Sa1(登录号JQ740599);Cry8Ta1(登录号KC156673);Cry8样(登录号FJ770571);Cry8样(登录号ABS53003);Cry9Aa1(登录号CAA41122);Cry9Aa2(登录号CAA41425);Cry9Aa3(登录号GQ249293);Cry9Aa4(登录号GQ249294);Cry9Aa5(登录号JX174110);Cry9Aa样(登录号AAQ52376);Cry9Ba1(登录号CAA52927);Cry9Ba2(登录号GU299522);Cry9Bb1(登录号AAV28716);Cry9Ca1(登录号CAA85764);Cry9Ca2(登录号AAQ52375);Cry9Da1(登录号BAA19948);Cry9Da2(登录号AAB97923);Cry9Da3(登录号GQ249293);Cry9Da4(登录号GQ249297);Cry9Db1(登录号AAX78439);Cry9Dc1(登录号KC156683);Cry9Ea1(登录号BAA34908);Cry9Ea2(登录号AAO12908);Cry9Ea3(登录号ABM21765);Cry9Ea4(登录号ACE88267);Cry9Ea5(登录号ACF04743);Cry9Ea6(登录号ACG63872);Cry9Ea7(登录号FJ380927);Cry9Ea8(登录号GQ249292);Cry9Ea9(登录号JN651495);Cry9Eb1(登录号CAC50780);Cry9Eb2(登录号GQ249298);Cry9Eb3(登录号KC156646);Cry9Ec1(登录号AAC63366);Cry9Ed1(登录号AAX78440);Cry9Ee1(登录号GQ249296);Cry9Ee2(登录号KC156664);Cry9Fa1(登录号KC156692);Cry9Ga1(登录号KC156699);Cry9样(登录号AAC63366);Cry10Aa1(登录号AAA22614);Cry10Aa2(登录号E00614);Cry10Aa3(登录号CAD30098);Cry10Aa4(登录号AFB18318);Cry10A样(登录号DQ167578);Cry11Aa1(登录号AAA22352);Cry11Aa2(登录号AAA22611);Cry11Aa3(登录号CAD30081);Cry11Aa4(登录号AFB18319);Cry11Aa样(登录号DQ166531);Cry11Ba1(登录号CAA60504);Cry11Bb1(登录号AAC97162);Cry11Bb2(登录号HM068615);Cry12Aa1(登录号AAA22355);Cry13Aa1(登录号AAA22356);Cry14Aa1(登录号AAA21516);Cry14Ab1(登录号KC156652);Cry15Aa1(登录号AAA22333);Cry16Aa1(登录号CAA63860);Cry17Aa1(登录号CAA67841);Cry18Aa1(登录号CAA67506);Cry18Ba1(登录号AAF89667);Cry18Ca1(登录号AAF89668);Cry19Aa1(登录号CAA68875);Cry19Ba1(登录号BAA32397);Cry19Ca1(登录号AFM37572);Cry20Aa1(登录号AAB93476);Cry20Ba1(登录号ACS93601);Cry20Ba2(登录号KC156694);Cry20样(登录号GQ144333);Cry21Aa1(登录号I32932);Cry21Aa2(登录号I66477);Cry21Ba1(登录号BAC06484);Cry21Ca1(登录号JF521577);Cry21Ca2(登录号KC156687);Cry21Da1(登录号JF521578);Cry22Aa1(登录号I34547);Cry22Aa2(登录号CAD43579);Cry22Aa3(登录号ACD93211);Cry22Ab1(登录号AAK50456);Cry22Ab2(登录号CAD43577);Cry22Ba1(登录号CAD43578);Cry22Bb1(登录号KC156672);Cry23Aa1(登录号AAF76375);Cry24Aa1(登录号AAC61891);Cry24Ba1(登录号BAD32657);Cry24Ca1(登录号CAJ43600);Cry25Aa1(登录号AAC61892);Cry26Aa1(登录号AAD25075);Cry27Aa1(登录号BAA82796);Cry28Aa1(登录号AAD24189);Cry28Aa2(登录号AAG00235);Cry29Aa1(登录号CAC80985);Cry30Aa1(登录号CAC80986);Cry30Ba1(登录号BAD00052);Cry30Ca1(登录号BAD67157);Cry30Ca2(登录号ACU24781);Cry30Da1(登录号EF095955);Cry30Db1(登录号BAE80088);Cry30Ea1(登录号ACC95445);Cry30Ea2(登录号FJ499389);Cry30Fa1(登录号ACI22625);Cry30Ga1(登录号ACG60020);Cry30Ga2(登录号HQ638217);Cry31Aa1(登录号BAB11757);Cry31Aa2(登录号AAL87458);Cry31Aa3(登录号BAE79808);Cry31Aa4(登录号BAF32571);Cry31Aa5(登录号BAF32572);Cry31Aa6(登录号BAI44026);Cry31Ab1(登录号BAE79809);Cry31Ab2(登录号BAF32570);Cry31Ac1(登录号BAF34368);Cry31Ac2(登录号AB731600);Cry31Ad1(登录号BAI44022);Cry32Aa1(登录号AAG36711);Cry32Aa2(登录号GU063849);Cry32Ab1(登录号GU063850);Cry32Ba1(登录号BAB78601);Cry32Ca1(登录号BAB78602);Cry32Cb1(登录号KC156708);Cry32Da1(登录号BAB78603);Cry32Ea1(登录号GU324274);Cry32Ea2(登录号KC156686);Cry32Eb1(登录号KC156663);Cry32Fa1(登录号KC156656);Cry32Ga1(登录号KC156657);Cry32Ha1(登录号KC156661);Cry32Hb1(登录号KC156666);Cry32Ia1(登录号KC156667);Cry32Ja1(登录号KC156685);Cry32Ka1(登录号KC156688);Cry32La1(登录号KC156689);Cry32Ma1(登录号KC156690);Cry32Mb1(登录号KC156704);Cry32Na1(登录号KC156691);Cry32Oa1(登录号KC156703);Cry32Pa1(登录号KC156705);Cry32Qa1(登录号KC156706);Cry32Ra1(登录号KC156707);Cry32Sa1(登录号KC156709);Cry32Ta1(登录号KC156710);Cry32Ua1(登录号KC156655);Cry33Aa1(登录号AAL26871);Cry34Aa1(登录号AAG50341);Cry34Aa2(登录号AAK64560);Cry34Aa3(登录号AAT29032);Cry34Aa4(登录号AAT29030);Cry34Ab1(登录号AAG41671);Cry34Ac1(登录号AAG50118);Cry34Ac2(登录号AAK64562);Cry34Ac3(登录号AAT29029);Cry34Ba1(登录号AAK64565);Cry34Ba2(登录号AAT29033);Cry34Ba3(登录号AAT29031);Cry35Aa1(登录号AAG50342);Cry35Aa2(登录号AAK64561);Cry35Aa3(登录号AAT29028);Cry35Aa4(登录号AAT29025);Cry35Ab1(登录号AAG41672);Cry35Ab2(登录号AAK64563);Cry35Ab3(登录号AY536891);Cry35Ac1(登录号AAG50117);Cry35Ba1(登录号AAK64566);Cry35Ba2(登录号AAT29027);Cry35Ba3(登录号AAT29026);Cry36Aa1(登录号AAK64558);Cry37Aa1(登录号AAF76376);Cry38Aa1(登录号AAK64559);Cry39Aa1(登录号BAB72016);Cry40Aa1(登录号BAB72018);Cry40Ba1(登录号BAC77648);Cry40Ca1(登录号EU381045);Cry40Da1(登录号ACF15199);Cry41Aa1(登录号BAD35157);Cry41Ab1(登录号BAD35163);Cry41Ba1(登录号HM461871);Cry41Ba2(登录号ZP_04099652);Cry42Aa1(登录号BAD35166);Cry43Aa1(登录号BAD15301);Cry43Aa2(登录号BAD95474);Cry43Ba1(登录号BAD15303);Cry43Ca1(登录号KC156676);Cry43Cb1(登录号KC156695);Cry43Cc1(登录号KC156696);Cry43样(登录号BAD15305);Cry44Aa(登录号BAD08532);Cry45Aa(登录号BAD22577);Cry46Aa(登录号BAC79010);Cry46Aa2(登录号BAG68906);Cry46Ab(登录号BAD35170);Cry47Aa(登录号AAY24695);Cry48Aa(登录号CAJ18351);Cry48Aa2(登录号CAJ86545);Cry48Aa3(登录号CAJ86546);Cry48Ab(登录号CAJ86548);Cry48Ab2(登录号CAJ86549);Cry49Aa(登录号CAH56541);Cry49Aa2(登录号CAJ86541);Cry49Aa3(登录号CAJ86543);Cry49Aa4(登录号CAJ86544);Cry49Ab1(登录号CAJ86542);Cry50Aa1(登录号BAE86999);Cry50Ba1(登录号GU446675);Cry50Ba2(登录号GU446676);Cry51Aa1(登录号ABI14444);Cry51Aa2(登录号GU570697);Cry52Aa1(登录号EF613489);Cry52Ba1(登录号FJ361760);Cry53Aa1(登录号EF633476);Cry53Ab1(登录号FJ361759);Cry54Aa1(登录号ACA52194);Cry54Aa2(登录号GQ140349);Cry54Ba1(登录号GU446677);Cry55Aa1(登录号ABW88932);Cry54Ab1(登录号JQ916908);Cry55Aa2(登录号AAE33526);Cry56Aa1(登录号ACU57499);Cry56Aa2(登录号GQ483512);Cry56Aa3(登录号JX025567);Cry57Aa1(登录号ANC87261);Cry58Aa1(登录号ANC87260);Cry59Ba1(登录号JN790647);Cry59Aa1(登录号ACR43758);Cry60Aa1(登录号ACU24782);Cry60Aa2(登录号EAO57254);Cry60Aa3(登录号EEM99278);Cry60Ba1(登录号GU810818);Cry60Ba2(登录号EAO57253);Cry60Ba3(登录号EEM99279);Cry61Aa1(登录号HM035087);Cry61Aa2(登录号HM132125);Cry61Aa3(登录号EEM19308);Cry62Aa1(登录号HM054509);Cry63Aa1(登录号BAI44028);Cry64Aa1(登录号BAJ05397);Cry65Aa1(登录号HM461868);Cry65Aa2(登录号ZP_04123838);Cry66Aa1(登录号HM485581);Cry66Aa2(登录号ZP_04099945);Cry67Aa1(登录号HM485582);Cry67Aa2(登录号ZP_04148882);Cry68Aa1(登录号HQ113114);Cry69Aa1(登录号HQ401006);Cry69Aa2(登录号JQ821388);Cry69Ab1(登录号JN209957);Cry70Aa1(登录号JN646781);Cry70Ba1(登录号ADO51070);Cry70Bb1(登录号EEL67276);Cry71Aa1(登录号JX025568);Cry72Aa1(登录号JX025569)。
δ-内毒素的实例还包括,但不限于美国专利号5,880,275和7,858,849的Cry1A蛋白;美国专利号8,304,604和8.304,605的DIG-3或DIG-11毒素(cry蛋白(如Cry1A)的α螺旋1和/或α螺旋2变体的N末端缺失),美国专利申请序列号10/525,318的Cry1B;美国专利号6,033,874的Cry1C;美国专利号5,188,960、6,218,188的Cry1F;美国专利号7,070,982、6,962,705和6,713,063的Cry1A/F嵌合体;Cry2蛋白,例如美国专利号7,064,249的Cry2Ab蛋白;Cry3A蛋白,包括但不限于:通过融合至少两种不同Cry蛋白的可变区和保守区的独特组合产生的工程化杂合杀昆虫蛋白(eHIP)(美国专利申请公开号2010/0017914);Cry4蛋白;Cry5蛋白;Cry6蛋白;美国专利号7,329,736、7,449,552、7,803,943、7,476,781、7,105,332、7,378,499和7,462,760的Cry8蛋白;Cry9蛋白,例如Cry9A、Cry9B、Cry9C、Cry9D、Cry9E和Cry9F家族的成员;Cry15蛋白,描述于以下文献中:Naimov等人(2008)Applied andEnvironmentalMicrobiology[应用与环境微生物学]74:7145-7151;美国专利号6,127,180、6,624,145和6,340,593的Cry22、Cry34Ab1蛋白;美国专利号6,248,535、6,326,351、6,399,330、6,949,626、7,385,107和7,504,229的CryET33和CryET34蛋白;美国专利公开号2006/0191034、2012/0278954和PCT公开号WO 2012/139004的CryET33和CryET34同系物;美国专利号6,083,499、6,548,291和6,340,593的Cry35Ab1蛋白;Cry46蛋白、Cry51蛋白、Cry二元毒素;TIC901或相关毒素;US 2008/0295207的TIC807;PCT US 2006/033867的ET29、ET37、TIC809、TIC810、TIC812、TIC127、TIC128;美国专利公开号2016/0108428的TIC1100、TlC 860、TIC867、TIC868、TIC869和TIC836。美国专利号8,236,757的AXMI-027、AXMI-036和AXMI-038;US 7,923,602的AXMI-031、AXMI-039、AXMI-040、AXMI-049;WO 2006/083891的AXMI-018、AXMI-020和AXMI-021;WO 2005/038032的AXMI-010;WO 2005/021585的AXMI-003;US 2004/0250311的AXMI-008;US 2004/0216186的AXMI-006;US 2004/0210965的AXMI-007;US 2004/0210964的AXMI-009;US 2004/0197917的AXMI-014;US 2004/0197916的AXMI-004;WO 2006/119457的AXMI-028和AXMI-029;WO 2004/074462的AXMI-007、AXMI-008、AXMI-0080rf2、AXMI-009、AXMI-014和AXMI-004;美国专利号8,084,416的AXMI-150;US20110023184的AXMI-205;US 2011/0263488的AXMI-011、AXMI-012、AXMI-013、AXMI-015、AXMI-019、AXMI-044、AXMI-037、AXMI-043、AXMI-033、AXMI-034、AXMI-022、AXMI-023、AXMI-041、AXMI-063和AXMI-064;US 2010/0197592的AXMI-R1和相关蛋白;WO 2011/103248的AXMI221Z、AXMI222z、AXMI223z、AXMI224z和AXMI225z;WO 11/103247的AXMI218、AXMI219、AXMI220、AXMI226、AXMI227、AXMI228、AXMI229、AXMI230和AXMI231;美国专利号8,334,431的AXMI-115、AXMI-113、AXMI-005、AXMI-163和AXMI-184;US 2010/0298211的AXMI-001、AXMI-002、AXMI-030、AXMI-035和AXMI-045;US 20090144852的AXMI-066和AXMI-076;美国专利号8,318,900的AXMI168、AXMI166、AXMI167、AXMI168、AXMI166、AXMI167、AXMI168、AXMI166、AXMI168、AXMI168、AXMI168、AXMI168、AXMI168、AXMI170、AXMI171、AXMI172、AXMI173、AXMI174、AXMI175、AXMI176、AXMI177、AXMI178、AXMI179、AXMI180、AXMI181、AXMI182、AXMI185、AXMI186、AXMI187、AXMI188、AXMI189;US 2010/0005543的AXMI079、AXMI080、AXMI081、AXMI082、AXMI091、AXMI092、AXMI096、AXMI097、AXMI098、AXMI099、AXMI100、AXMI101、AXMI102、AXMI103、AXMI104、AXMI107、AXMI108、AXMI109、AXMI110、AXMI111、AXMI112、AXMI114、AXMI116、AXMI117、AXMI118、AXMI119、AXMI120、AXMI121、AXMI122、AXMI123、AXMI124、AXMI1257、AXMI1268、AXMI127、AXMI129、AXMI164、AXMI151、AXMI161、AXMI183、AXMI132、AXMI138、AXMI137;和Cry蛋白(如Cry1A和Cry3A),其具有美国专利号8,319,019的经修饰的蛋白水解位点;美国专利申请公开号2011/0064710的来自苏芸金杆菌菌株VBTS2528的Cry1Ac、Cry2Aa和Cry1Ca毒素蛋白,和PCT公开号WO 2016/061197的IP1B。其他Cry蛋白是本领域技术人员熟知的(参见Crickmore等人,“Bacillusthuringiensis toxin nomenclature[苏云金芽孢杆菌毒素命名法]”(2011),网址为lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/,可以使用“www”前缀在万维网上访问)。Cry蛋白的杀昆虫活性是本领域技术人员所熟知的(回顾参见van Frannkenhuyzen,(2009)J.Invert.Path.[无脊椎动物病理学杂志]101∶1-16)。使用Cry蛋白作为转基因植物性状是本领域技术人员所熟知的,并且Cry转基因植物(包括但不限于Cry1Ac、Cry1Ac+Cry2Ab、Cry1Ab、Cry1A.105、Cry1F、Cry1Fa2、Cry1F+Cry1Ac、Cry2Ab、Cry3A、mCry3A、Cry3Bb1、Cry34Ab1、Cry35Ab1、Vip3A、mCry3A、Cry9c和CBI-Bt)已获得监管部门的批准(参见,Sanahuja,(2011)Plant Biotech Journal[植物生物技术杂志]9∶283-300和CERA(2010)转基因作物数据库环境风险评估中心(CERA)(GM Crop Database Center forEnvironmental Risk Assessment),ILSI研究基金会,华盛顿特区,网址为cera-gme.org/index.php?action=gm_crop_database,可以使用“www”前缀在万维网上访问)。本领域技术人员熟知的多种杀有害生物蛋白也可以在如下植物中表达:如Vip3Ab&Cry1Fa(US 2012/0317682)、Cry1BE&Cry1F(US2012/0311746)、Cry1CA&Cry1AB(US 2012/0311745)、Cry1F&CryCa(US 2012/0317681)、Cry1DA&Cry1BE(US 2012/0331590)、Cry1DA&Cry1Fa(US 2012/0331589)、Cry1AB&Cry1BE(US 2012/0324606)以及Cry1Fa&Cry2Aa、Cry1I或Cry1E(US2012/0324605));Cry34Ab/35Ab和Cry6Aa(US 20130167269);Cry34Ab/VCry35Ab&Cry3Aa(US 20130167268);Cry3A和Cry1Ab或Vip3Aa(US 20130116170);以及Cry1F、Cry34Ab1和Cry35Ab1(PCT/US2010/060818)。杀有害生物蛋白还包括杀昆虫脂肪酶,该杀昆虫脂肪酶包括美国专利号7,491,869的脂质酰基水解酶,以及胆固醇氧化酶,例如来自链霉菌(Purcell等人,(1993)Biochem Biophys Res Commun[生物化学与生物物理研究通讯]15∶1406-1413)。杀有害生物蛋白还包括美国专利号5,877,012、6,107,279、6,137,033、7,244,820、7,615,686和8,237,020中的VIP(营养性杀昆虫蛋白)毒素等。其他VIP蛋白质是本领域技术人员所熟知的(参见lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/vip.html,可以使用“www”前缀在万维网上访问)。杀有害生物蛋白还包括可从如下生物体获得的毒素复合物(TC)蛋白质:致病杆菌、发光杆菌和类芽孢杆菌(参见美国专利号7,491,698和8,084,418)。一些TC蛋白具有“独立”杀昆虫活性,并且其他TC蛋白增强了由相同给定生物体产生的独立毒素的活性。可以通过源自不同属的来源生物体的一种或多种TC蛋白“增效剂”来增强“独立”TC蛋白(例如来自发光杆菌属、致病杆菌属或类芽孢杆菌属)的毒性。有三种主要类型的TC蛋白。如本文所提及的,A类蛋白(“蛋白A”)是独立毒素。B类蛋白(“蛋白B”)和C类蛋白(“蛋白C”)提高了A类蛋白的毒性。A类蛋白的实例是TcbA、TcdA、XptA1和XptA2。B类蛋白的实例是TcaC、TcdB、XptB1Xb和XptC1Wi。C类蛋白的实例是TccC、XptC1Xb和XptB1Wi。杀有害生物蛋白还包括蜘蛛、蛇和蝎毒蛋白。蜘蛛肽的实例包括但不限于莱科毒素-1肽及其突变体(美国专利号8,334,366)。
(C)编码昆虫特异性激素或信息素(如蜕化类固醇和保幼激素)的多核苷酸,其变体,基于其的模拟物,或者其拮抗剂或激动剂。参见,例如Hammock等人(1990)Nature[自然]344∶458,该文献公开了经克隆的保幼激素酯酶的杆状病毒表达是保幼激素的灭活剂。
(D)编码昆虫特异性肽的多核苷酸,其在表达时破坏受影响有害生物的生理机能。例如,参见Regan,(1994)J.Biol.Chem.[生物化学杂志]269∶9的公开内容(表达克隆产生编码昆虫利尿激素受体的DNA);Pratt等人,(1989)Biochem.Biophys.Res.Comm.[生物化学与生物物理研究通讯]163∶1243(在Diploptera puntata中识别出了阿洛斯德汀(allostatin));Chattopadhyay等人,(2004)Critical Reviews in Microbiology[微生物学评论]30(1):33-54;Zjawiony,(2004)J Nat Prod[天然产物杂志]67(2):300-310;Carlini和Grossi-de-Sa,(2002)Toxicon[毒素]40(11):1515-1539;Ussuf等人,(2001)Curr Sci.[当代科学]80(7):847-853以及Vasconcelos和Oliveira,(2004)Toxicon[毒素]44(4):385-403。还参见,美国专利号5,266,317,作者Tomalski等人,他们披露了编码昆虫特异性毒素的基因。
(E)编码如下酶的多核苷酸,该多核苷酸负责单萜、倍半萜烯、类固醇、异羟肟酸、苯丙素衍生物或具有杀昆虫活性的其他非蛋白质分子的超累积。
(F)编码参与生物活性分子的修饰(包括翻译后修饰)的酶的多核苷酸;例如糖酵解酶、蛋白水解酶、脂肪分解酶、核酸酶、环化酶、转氨酶、酯酶、水解酶、磷酸酶、激酶、磷酸化酶、聚合酶、弹性蛋白酶、几丁质酶和葡聚糖酶,无论是天然还是合成的。参见,PCT申请WO1993/02197,所属人为Scott等人,该文献披露了调节酶(callase)基因的核苷酸序列。含有几丁质酶编码序列的DNA分子可以例如从登录号39637和67152下的ATCC获得。还参见,Kramer等人,(1993)Insect Biochem.Molec.Biol.[分子细胞生物学]23:691,他们教导了编码烟草钩虫几丁质酶的cDNA的核苷酸序列和Kawalleck等人,(1993)Plant Molec.Biol.[分子细胞生物学]21:673,他们提供了欧芹ubi4-2聚泛素基因的核苷酸序列,以及美国专利号6,563,020、7,145,060和7,087,810。
(G)编码刺激信号转导的分子的多核苷酸。例如,参见Botella等人,(1994)PlantMolec.Biol.[分子细胞生物学]24:757,该文献公开了绿豆钙调素cDNA克隆的核苷酸序列,以及Griess等人,(1994)Plant Physiol.[植物生理学]104:1467,他们提供了玉米钙调素cDNA克隆的核苷酸序列。
(H)编码疏水时刻肽(moment peptide)的多核苷酸。参见,PCT申请WO 1995/16776和美国专利号5,580,852(公开了抑制真菌植物病原体的鲎素(Tachyplesin)的肽衍生物)以及PCT申请WO 1995/18855和美国专利号5,607,914(教导了赋予抗病性的合成抗微生物肽)。
(I)编码膜通透酶、通道形成剂(channel former)或通道阻断剂的多核苷酸。例如,参见Jaynes等人,(1993)Plant Sci.[植物科学]89∶43,该文献公开了天蚕素-β裂解肽类似物的异源表达,以提供对烟草假单胞菌具有抗性的转基因烟草植物。
(J)编码病毒侵入性蛋白质的基因或由其衍生的复合毒素。例如,病毒外壳蛋白在转化的植物细胞中的积累赋予由外源蛋白基因来源的病毒以及由相关病毒导致的病毒侵染和/或疾病发展的抗性。参见,Beachy等人,(1990)Ann.Rev.Phytopathol.[植物病理学年评]28∶451。外壳蛋白介导的抗性已被赋予至转化植物抵抗以下各项:苜蓿花叶病毒、黄瓜花叶病毒、烟草线条病毒、马铃薯X病毒、马铃薯Y病毒、烟草蚀纹病毒、烟草脆裂病毒和烟草花叶病毒。同上。
(K)编码昆虫特异性抗体或由其衍生的免疫毒素的基因。因此,靶向昆虫肠道中关键代谢功能的抗体将使受影响的酶失活,杀死该昆虫。参看Taylor等人,摘要#497,关于分子植物-微生物相互作用的第七届国际研讨会(SEVENTH INT′L SYMPOSIUM ON MOLECULARPLANT-MICROBE INTERACTIONS)(苏格兰爱丁堡(Edinburgh,Scotland),1994)(通过生产单链抗体片段在转基因烟草中的酶失活)。
(L)编码病毒特异性抗体的基因。参见,例如Tavladoraki等人,(1993)Nature[自然]366∶469,其作者显示,表达重组抗体基因的转基因植物不受病毒侵袭。
(M)编码由病原体或寄生虫在自然中产生的发育阻滞蛋白的多核苷酸。因此,真菌内α-1,4-D-多聚半乳糖醛酸酶通过溶解植物细胞壁homo-α-1,4-D-半乳糖醛酸酶来促进真菌定植和植物营养释放。参见Lamb等人,(1992)Bio/Technology[生物技术]10∶1436。以下文献描述了编码豆内聚半乳糖醛酸酶抑制蛋白的基因的克隆和表征:Toubart等人,(1992)Plant J.[植物杂志]2:367。
(N)编码由植物在自然中产生的发育抑制蛋白的多核苷酸。例如,已在以下文献中表明表达大麦核糖体失活基因的转基因植物具有增加的对真菌疾病的抗性:Logemann等人,(1992)Bio/Technology[生物技术]10∶305。
(O)参与系统获得抗性(SAR)反应的基因和/或发病机制相关的基因。Briggs,(1995)Current Biology[当代生物学]5(2),Pieterse和Van Loon,(2004)Curr.Opin.Plant Bio.[植物生物学新观点]7(4):456-64,以及Somssich,(2003)Cell[细胞]113(7):815-6。
(P)抗真菌基因(Comelissen和Melchers,(1993)Pl.Physiol.[植物生理学]101∶709-712,和Parijs等人,(1991)Planta[植物]183∶258-264,以及Bushnell等人,(1998)Can.J.ofPlant Path.[加拿大植物病理学杂志]20(2):137-149)。还参见,美国专利申请序列号09/950,933;11/619,645;11/657,710;11/748,994;11/774,121以及美国专利号6,891,085和7,306,946。LysM受体样激酶用于感知几丁质片段,作为植物对真菌病原体防御反应的第一步(US2012/0110696)。
(Q)解毒基因,如伏马菌素、白僵菌素、念珠菌素和玉米赤霉烯酮及其结构相关的衍生物的基因。例如,参见美国专利号5,716,820;5,792,931;5,798,255;5,846,812;6,083,736;6,538,177;6,388,171和6,812,380。
(R)编码胱抑素和半胱氨酸蛋白酶抑制剂的多核苷酸。参见,美国专利号7,205,453。
(S)防御素基因。参见WO 2003/000863和美国专利号6,911,577;6,855,865;6,777,592和7,238,781。
(T)赋予线虫抗性的基因。参见,例如PCT申请WO 1996/30517;PCT申请WO 1993/19181、WO 2003/033651,以及Urwin等人,(1998)Planta[植物]204:472-479,和Williamson,(1999)Curr Opin Plant Bio.[植物生物学新观点]2(4):327-31;美国专利号6,284,948和7,301,069,和miR164基因(WO2012/058266)。
(U)赋予对疫霉根腐病抗性的基因,如Rps 1、Rps 1-a、Rps 1-b、Rps1-c、Rps 1-d、Rps 1-e、Rps 1-k、Rps 2、Rps 3-a、Rps 3-b、Rps 3-c、Rps 4、Rps 5、Rps 6、Rps 7和其他Rps基因。参见,例如Shoemaker等人,Phytophthora Root Rot Resistance Gene Mappingin Soybean[大豆中疫霉根腐病抗性基因图谱],植物基因组第四次会议,加利福尼亚州圣地亚哥(San Diego,Calif.)(1995)。
(V)赋予对褐茎腐病抗性的基因,如美国专利号5,689,035所述,并为此通过引入并入本文。
(W)赋予对炭疽菌抗性的基因,如美国专利申请公开US 2009/0035765中所述,并为此通过引用并入本文。这包括可以用作单一基因座转化的Rcg基因座。
(X)一些实施例涉及通过干扰核糖核酸(RNA)分子来下调昆虫有害生物物种中靶基因的表达。PCT公开WO 2007/074405描述了抑制包括科罗拉多马铃薯甲虫在内的无脊椎动物有害生物中靶基因表达的方法。PCT公开WO 2005/110068描述了抑制无脊椎动物有害生物(特别是包括西方玉米根虫)中靶基因表达的方法,该方法作为防治昆虫侵染的手段。此外,PCT公开WO 2009/091864描述了用于抑制来自昆虫有害生物物种(包括来自草盲蝽属的有害生物)的靶基因的组合物和方法。
核酸分子包括用于靶向液泡ATP酶H亚基的沉默元件,该沉默元件可用于防治如美国专利申请公开号2012/0198586中所述的鞘翅目有害生物群体和侵袭。PCT公开WO 2012/055982描述了抑制或下调如下靶基因的表达的核糖核酸(RNA或双链RNA),该靶基因编码:昆虫核糖体蛋白,如核糖体蛋白L19、核糖体蛋白L40或核糖体蛋白S27A;昆虫蛋白酶体亚基,如Rpn6蛋白,Pros 25,Rpn2蛋白,蛋白酶体p1亚基蛋白或Prosβ2蛋白;COPI囊泡的昆虫β-外被体,COPI囊泡的γ-外被体,COPI囊泡的β′-外被体蛋白或ζ-外被体;昆虫四跨膜蛋白2A蛋白,其是假定跨膜结构域蛋白;属于肌动蛋白家族的昆虫蛋白,如肌动蛋白5C;昆虫泛素-5E蛋白;昆虫Sec23蛋白(参与细胞内蛋白质运输的GTP酶激活子);涉及运动活性的作为非常规肌球蛋白的昆虫皱纹蛋白质;涉及核替代mRNA剪接调节的昆虫曲颈蛋白;昆虫液泡H+-ATP酶G亚基蛋白和昆虫Tbp-1如Tat结合蛋白。PCT公开WO 2007/035650描述了抑制或下调编码Snf7的靶基因表达的核糖核酸(RNA或双链RNA)。美国专利申请公开2011/0054007描述了靶向RPS10的多核苷酸沉默元件。美国专利申请公开2014/0275208和US 2015/0257389描述了靶向RyanR和PAT3的多核苷酸沉默元件。PCT公开WO 2016/060911、WO 2016/060912、WO 2016/060913、和WO 2016/060914描述了靶向赋予对鞘翅目和半翅目有害生物的抗性的COPI衣壳亚单位核酸分子的多核苷酸沉默元件。美国专利申请公开2012/029750、US20120297501和2012/0322660描述了干扰核糖核酸(RNA或双链RNA),该干扰核糖核酸在被昆虫有害生物物种摄取时起作用以下调所述昆虫有害生物中靶基因的表达,其中该RNA包含至少一个沉默元件,其中该沉默元件是包含经退火的互补链的双链RNA区域,所述双链RNA区域的一条链包含或由如下核苷酸序列组成,该核苷酸序列至少部分地与靶基因中的靶标核苷酸序列互补。美国专利申请公开2012/0164205描述了用于干扰双链核糖核酸(用于抑制无脊椎动物有害生物)的潜在靶标,包括:Chd3同源序列、β-微管蛋白同源序列、40kDa V-ATP酶同源序列、EF1α同源序列、26S蛋白质体亚基p28同源序列、保幼激素环氧化物酶水解酶同源序列、溶胀依赖氯通道蛋白同源序列、葡萄糖-6-磷酸1-脱氢酶蛋白同源序列、Act42A蛋白同源序列、ADP-核糖因子1同源序列、转录因子IIB蛋白同源序列、几丁质酶同源序列、泛素缀合酶同源序列、甘油醛-3-磷酸脱氢酶同源序列、泛素B同源序列、保幼激素酯酶同源物、和α微管蛋白同源序列。
ii.赋予除草剂抗性的转基因
(A)编码对如下除草剂的抗性的多核苷酸,该除草剂抑制生长点或分生组织,如咪唑啉酮或磺酰脲。此分类码中的示例性基因突变体ALS和AHAS酶,分别如以下文献中所述:Lee等人,(1988)EMBO J.[欧洲分子生物学学会杂志]7:1241和Miki等人,(1990)Theor.Appl.Genet.[理论与应用遗传学]80:449。还参见美国专利号5,605,011;5,013,659;5,141,870;5,767,361;5,731,180;5,304,732;4,761,373;5,331,107;5,928,937和5,378,824;美国专利申请序号11/683,737和国际公开WO 1996/33270。
(B)编码草甘膦抗性的蛋白质的多核苷酸(5-烯醇式丙酮酰-3-磷酸莽草酸合酶(EPSP)和aroA基因给予的抗性)和其他膦酰基化合物如草胺膦(草胺膦乙酰转移酶(PAT)和吸水链霉菌草胺膦乙酰转移酶(bar)基因),以及吡啶氧基或苯氧基丙酸和环己酮(ACC酶抑制剂编码基因)。参见,例如Shah等人的美国专利号4,940,835,其披露了EPSPS形式的能赋予草甘磷抗性的核苷酸序列。Barry等人的美国专利号5,627,061也描述了编码EPSPS酶的基因。还参见美国专利号6,566,587;6,338,961;6,248,876B1;6,040,497;5,804,425;5,633,435;5,145,783;4,971,908;5,312,910;5,188,642;5,094,945、4,940,835;5,866,775;6,225,114B1;6,130,366;5,310,667;4,535,060;4,769,061;5,633,448;5,510,471;Re.36,449;RE37,287E和5,491,288以及国际公开EP 1173580;WO 2001/66704;EP1173581和EP 1173582中。
还给予植物草甘磷抗性,使该植物表达编码草甘磷氧化还原酶的基因,这在美国专利号5,776,760和5,463,175中进行了更全面地描述。另外,可通过过量表达编码草甘磷N-乙酰转移酶的基因,来赋予植物草甘磷抗性。参见,例如美国专利号7,462,481;7,405,074以及美国专利申请公开号2008/0234130。编码突变areA基因的DNA分子可以在ATCC登录号39256下获得,并且该突变基因的核苷酸序列披露于授予Comai的美国专利号4,769,061中。欧洲申请号0333033(Kumada等人)和美国专利号4,975,374(Goodman等人)披露了赋予除草剂(如L-草铵膦)抗性的谷氨酰胺合成酶基因的核苷酸序列。Leemans等人的EP申请号0242 246和0 242 236中提供了草丁膦乙酰基转移酶基因的核苷酸序列;De Greef等人,(1989)Bio/Technology[生物/技术]7:61,描述了表达编码草丁膦乙酰转移酶活性的嵌合bar基因的转基因植物的产生。还参见美国专利号5,969,213;5,489,520;5,550,318;5,874,265;5,919,675;5,561,236;5,648,477;5,646,024;6,177,616B1和5,879,903。赋予苯氧基丙酸和环己酮(如稀禾定和吡氟氯禾灵)抗性的示例性基因是Acc1-S1、Acc1-S2和Acc1-S3基因,其描述于以下文献中:Marshall等人,(1992)Theor.Appl.Genet.[理论与应用遗传学]83:435。
(C)编码对抑制光合作用的除草剂具有抗性的蛋白质的多核苷酸,例如三嗪(psbA和gs+基因)和苄腈(腈水解酶基因)。Przibilla等人(1991)Plant Cell[植物细胞]3∶169,描述了用编码突变体psbA基因的质粒对衣藻进行转化。针对腈水解酶基因的核苷酸序列披露在美国专利号4,810,648(Stalker)中,并且含有这些基因的DNA分子可以在ATCC登录号53435,67441和53435,67442下获得。编码谷胱甘肽S-转移酶的DNA的克隆和表达描述于以下文献中:Hayes等人,(1992)Biochem.J.[生物化学杂志]285:173。
(D)已经发现,编码对乙酰醇酸合成酶具有抗性的蛋白质的多核苷酸能产生表达这种对多种类型的除草剂具有抗性的酶的植物,并已将该多核苷酸引入到多种植物中(参见,例如Hattori等人(1995)Mol Gen Genet.[分子和普通遗传学]246:419)。赋予除草剂抗性的其他基因包括:编码大鼠细胞色素P4507A1和酵母NADPH-细胞色素P450氧化还原酶的嵌合蛋白的基因(Shiota等人,(1994)Plant Physiol[植物生理学]106:17),针对谷胱甘肽还原酶和超氧化物歧化酶的基因(Aono等人,(1995)Plant Cell Physiol[植物细胞生理学]36:1687)和各种磷酸转移酶的基因(Datta等人,(1992)Plant Mol Biol[植物分子生理学]20:619)。
(E)编码靶向原卟啉原氧化酶(protox)(对于生产叶绿素所必需的)的除草剂抗性的多核苷酸。原卟啉原氧化酶(protox)作为针对各种除草化合物的靶标。这些除草剂还抑制存在的所有不同种类的植物的生长,导致其完全破坏。含有对这些除草剂具有抗性的经改变的原卟啉原氧化酶(protox)活性的植物的发育描述于以下文献中:美国专利号6,288,306B1;6,282,837B1和5,767,373,以及国际公开WO 2001/12825。
(F)aad-1基因(最初来自鞘脂单胞菌(Sphingobium herbicidovorans))编码芳氧基链烷酸酯双加氧酶(AAD-1)蛋白。该性状赋予对2,4-二氯苯氧基乙酸和芳氧基苯氧基丙酸酯(通常称为“fop”除草剂,例如喹禾灵)除草剂的耐受性。用于植物中除草剂耐受性的aad-1基因本身首先在WO 2005/107437中披露(还参见US 2009/0093366)。来自食酸丛毛单胞菌的aad-12基因,其编码芳氧基链烷酸酯双加氧酶(AAD-12)蛋白,该蛋白通过用芳氧基链烷酸酯部分(包括苯氧基生长素(例如2,4-D,MCPA)以及吡啶氧基生长素(例如氯氟吡氧乙酸,三氯吡氧乙酸))使几种除草剂失活来赋予对2,4-二氯苯氧基乙酸和吡啶氧基乙酸酯除草剂的耐受性。
(G)美国专利申请公开2003/0135879中所披露的用于给予麦草畏耐受性的编码除草剂抗性的麦草畏单加氧酶的多核苷酸。
(H)美国专利号4,810,648中所披露的用于给予溴苯腈耐受性的编码溴苯腈腈水解酶(Bxn)的多核苷酸分子。
(I)用于达草灭耐受性的编码八氢番茄红素(crt1)的多核苷酸分子描述于以下文献中:Misawa等人,(1993)Plant J.[植物杂志]4:833-840和Misawa等人,(1994)Plant J.[植物杂志]6:481-489。
iii.赋予或贡献于经改变的谷物特征的转基因
(A)经改变的脂肪酸,例如,通过(1)硬脂酰-ACP的下调来增加植物的硬脂酸含量。参见,Knultzon等人,(1992)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]89:2624和WO1999/64579(Genes to Alter Lipid Profiles in Corn[改变玉米脂质谱的基因]);(2)通过FAD-2基因修饰提高油酸和/或通过FAD-3基因修饰降低亚麻酸(参见美国专利号6,063,947;6,323,392;6,372,965以及WO 1993/11245);(3)改变共轭的亚麻酸或亚油酸含量,如WO 2001/12800中;(4)改变LEC1、AGP、Dek1、Superal1、mil ps、各种Ipa基因(如Ipal、Ipa3、hpt或hggt)。例如,参见WO 2002/42424,WO 1998/22604,WO 2003/011015,WO 2002/057439,WO 2003/011015,美国专利号6,423,886、6,197,561、6,825,397,和美国专利申请公开号US2003/0079247、US 2003/0204870,以及Rivera-Madrid等人,(1995)Proc.Natl.Acad.Sci.[国家科学院院刊]92:5620-5624;(5)编码用于制备长链多不饱和脂肪酸的δ-8去饱和酶的基因(美国专利号8,058,571和8,338,152),用于降低饱和脂肪的δ-9去饱和酶(美国专利号8,063,269),用于改善ω-3脂肪酸谱的报春花δ6-去饱和酶;(6)与脂质和糖代谢调节相关的分离的核酸和蛋白质,特别是用于生产转基因植物和调节种子储存化合物(包括脂质、脂肪酸、淀粉或种子储存蛋白)的水平的方法中以及并用于调节植物种子大小、种子数、种子重量、根长和叶子大小的方法中的脂质代谢蛋白(LMP)(EP2404499);(7)改变植物中糖诱导型2(HSI2)蛋白的高水平表达,以增加或减少植物中HSI2的表达。增加HSI2的表达增加油含量,然而降低HSI2的表达降低脱落酸敏感性和/或增加抗旱性(美国专利申请公开号2012/0066794);(8)细胞色素b5(Cb5)单独的或与FAD2一起的表达调节植物种子中的油含量,特别是提高ω-3脂肪酸的水平,并提高ω-6脂肪酸与ω-3脂肪酸的比例(美国专利申请公开号2011/0191904);以及(9)编码用于调节糖代谢的皱纹1样多肽的核酸分子(美国专利号8,217,223)。
(B)经改变的磷含量,例如,通过(1)引入植酸酶编码基因将促进植酸盐的分解,向经转化的植物中添加更多的游离磷酸盐。例如,参见Van Hartingsveldt等人,(1993)Gene[基因]127:87,该文献公开了黑曲霉植酸酶基因的核苷酸序列;和(2)调节降低植酸盐含量的基因。例如,可以在玉米中通过以下方法来完成:克隆然后重新引入与一个或多个等位基因相关联的DNA,例如在以低水平的植酸为特征的玉米突变体中经识别的LPA等位基因,例如WO 2005/113778中;和/或改变肌醇激酶活性,如WO 2002/059324、美国专利申请公开号2003/0009011、WO 2003/027243、美国专利申请公开号2003/0079247、WO 1999/05298、美国专利号6,197,561、美国专利号6,291,224、美国专利号6,391,348、WO 2002/059324、美国专利申请公开号2003/0079247、WO 1998/45448、WO 1999/55882、WO 2001/04147中。
(C)经改变的受到影响的碳水化合物,例如通过改变如下基因:影响淀粉分支模式的酶的基因,或改变硫氧还蛋白(如NTR和/或TRX)(参见美国专利号6,531,648,为此目的通过引用并入本文)和/或γ玉米蛋白敲除或突变体(如cs27或TUSC27或者en27)(参见美国专利号6,858,778和美国专利申请公开号2005/0160488、美国专利申请公开号2005/0204418,其通过引用并入本文)的基因。参见,Shiroza等人,(1988)J.Bacteriol.[细菌学杂志]170:810(链球菌突变体果糖基转移酶基因的核苷酸序列),Steinmetz等人,(1985)Mol.Gen.Genet.[分子遗传学和基因组学]200:220(枯草芽孢杆菌果聚糖蔗糖酶基因的核苷酸序列),Pen等人,(1992)Bio/Technology[生物技术]10:292(表达地衣芽孢杆菌α-淀粉酶的转基因植物的生产),Elliot等人,(1993)Plant Molec.Biol.[植物分子生物学]21:515(番茄转化酶基因的核苷酸序列),Sogaard等人,(1993)J.Biol.Chem.[生物化学杂志]268:22480(大麦α-淀粉酶基因的定点诱变)以及Fisher等人,(1993)Plant Physiol.[植物生理学]102:1045(玉米胚乳淀粉分支酶II),WO 1999/10498(通过修饰UDP-D-木糖4-差向异构酶、Fragile 1和2、Ref1、HCHL、C4H改善消化性和/或淀粉提取),美国专利号6,232,529(通过改变淀粉水平生产高油种子的方法(AGP))。本文提及的脂肪酸修饰基因也可用于通过淀粉和油途径的相互关系来影响淀粉含量和/或组成。
(D)改变的抗氧化剂含量或组成,如改变生育酚或生育三烯酚。例如,参见美国专利号6,787,683、美国专利申请公开号2004/0034886和WO 2000/68393、以及WO 2003/082899,这些专利涉及通过改变尿黑酸香叶基香叶基转移酶(hggt)来操作抗氧化剂水平。
(E)改变的必需种子氨基酸。例如,参见美国专利号6,127,600(增加种子中必需氨基酸积累的方法)、美国专利号6,080,913(增加种子中必需氨基酸积累的二元方法)、美国专利号5,990,389(高赖氨酸)、WO 1999/40209(种子中氨基酸组成的改变)、WO 1999/29882(用于改变蛋白质的氨基酸含量的方法)、美国专利号5,850,016(种子中氨基酸组成的改变)、WO 1998/20133(具有升高水平的必需氨基酸的蛋白质)、美国专利号5,885,802(高甲硫氨酸)、美国专利号5,885,801(高苏氨酸)、美国专利号6,664,445(植物氨基酸生物合成酶)、美国专利号6,459,019(赖氨酸和苏氨酸增加)、美国专利号6,441,274(植物色氨酸合酶β亚基)、美国专利号6,346,403(甲硫氨酸代谢酶)、美国专利号5,939,599(高硫)、美国专利号5,912,414(甲硫氨酸增加)、WO 1998/56935(植物氨基酸生物合成酶)、WO 1998/45458(具有较高百分比的必需氨基酸的工程化种子蛋白)、WO 1998/42831(赖氨酸增加)、美国专利号5,633,436(增加含硫氨基酸含量)、美国专利号5,559,223(具有含可编程水平的必需氨基酸的明确定义的结构的合成储存蛋白,用于改善植物的营养价值)、WO 1996/01905(苏氨酸增加)、WO 1995/15392(赖氨酸增加)、美国专利申请公开号2003/0163838、美国专利申请公开号2003/0150014、美国专利申请公开号2004/0068767、美国专利号6,803,498、WO2001/79516。
iv.控制雄性不育的基因
有几种授予遗传雄性不育的方法,例如授予雄性不育的基因组内单独位置处的多个突变基因,如Brar等人在美国专利号4,654,465和4,727,219中所披露的,以及染色体易位,如Patterson在美国专利号3,861,709和3,710,511中所述。除了这些方法之外,Albertsen等人在美国专利号5,432,068中描述了细胞核雄性不育的系统,该系统包括:识别对雄性能育性至关重要的基因;沉默这种对雄性能育性至关重要的天然基因;从必需的雄性能育性基因中除去天然启动子并用诱导型启动子取代;将该遗传工程化基因插回入植物;并因此产生雄性不育的植物,因为诱导型启动子不是“开”的,导致雄性能育性基因不被转录。通过诱导或将启动子打“开”来恢复能育性,该启动子反过来又允许赋予雄性能育性的基因被转录。非限制性实例包括:(A)在绒毡层特异性启动子的控制下并施用化学品N-Ac-PPT来引入脱乙酰酶基因(WO 2001/29237);(B)引入各种雄蕊特异性启动子(WO 1992/13956,WO 1992/13957);和(C)引入barnase和barstar基因(Paul等人,(1992)PlantMol.Biol.[植物分子生物学]19:611-622)。关于细胞核雄性和雌性不育系统和基因的另外的实例,也可参见美国专利号5,859,341;6,297,426;5,478,369;5,824,524;5,850,014和6,265,640。
v.创建用于位点特异性DNA整合的位点的基因
这包括引入可能用于FLP/FRT系统中的FRT位点和可能用于Cre/Loxp系统中的Lox位点。例如,参见Lyznik等人(2003)Plant Cell Rep[植物细胞报告]21:925-932和WO1999/25821。可以使用的其他系统包括噬菌体Mu的Gln重组酶(Maeser等人,(1991)VickiChandler,The Maize Handbook[玉米手册]ch.118(施普林格出版公司(Springer-Verlag)1994)、大肠杆菌的Pin重组酶(Enomoto等人,1983)和pSRi质粒的R/RS系统(Araki等人,1992)。
vi.影响非生物胁迫抗性的基因
包括但不限于开花、穗和种子发育、提高氮利用效率、改变氮反应性、抗旱性或耐旱性、抗寒性或耐寒性、抗盐性或耐盐性以及在胁迫下产量增加。非限制性实例包括:(A)例如,参见:WO 2000/73475,其中通过改变苹果酸来改变水分的使用效率;美国专利号5,892,009、5,965,705、5,929,305、5,891,859、6,417,428、6,664,446、6,706,866、6,717,034、6,801,104,WO 2000/060089,WO 2001/026459,WO 2001/035725,WO 2001/034726,WO 2001/035727,WO 2001/036444,WO 2001/036597,WO 2001/036598,WO 2002/015675,WO 2002/017430,WO 2002/077185,WO 2002/079403,WO 2003/013227,WO 2003/013228,WO 2003/014327,WO 2004/031349,WO 2004/076638,WO 199809521;(B)WO 199938977描述了基因(包括CBF基因)以及有效减轻冷冻、高盐度和干旱对植物的负面影响以及赋予植物表型其他积极作用的转录因子;(C)美国专利申请公开号2004/0148654和WO 2001/36596中,在植物中改变脱落酸,产生改良的植物表型,例如增加的产量和/或增加的对非生物胁迫的耐受性;(D)WO 2000/006341、WO 2004/090143、美国专利号7,531,723和6,992,237中,修饰细胞分裂素表达,产生具有增加的胁迫耐受性(例如耐旱性和/或增加的产量)的植物。还可参见,WO 2002/02776、WO 2003/052063、JP 2002/281975、美国专利号6,084,153、WO 2001/64898、美国专利号6,177,275和美国专利号6,107,547(提高氮利用率和改变氮反应性);(E)关于乙烯改变,参见美国专利申请公开号2004/0128719、美国专利申请公开号2003/0166197和WO 2000/32761;(F)关于非生物胁迫的植物转录因子或转录调节子,参见例如美国专利申请公开号2004/0098764或美国专利申请公开号2004/0078852;(G)增加液泡焦磷酸酶(如AVP1)表达的基因(美国专利号8,058,515),用于提高产量;编码HSFA4或HSFA5的核酸(A4或A5类的热休克因子)多肽,寡肽转运蛋白(OPT4样)多肽;Plastochron2样(PLA2样)多肽或Wuschel相关同源盒1样(WOX1样)多肽(美国专利申请公开号US 2011/0283420);(H)下调编码聚(ADP-核糖)聚合酶(PARP)蛋白的多核苷酸来调节程序性细胞死亡(美国专利号8,058,510),用于增加活力;(I)编码用于赋予抗旱性的DTP21多肽的多核苷酸(美国专利申请公开号US 2011/0277181);(J)编码ACC合酶3(ACS3)蛋白质的核苷酸序列,用于调节发育、调节胁迫应答和调节胁迫耐受性(美国专利申请公开号2010/0287669);(K)编码赋予耐旱表型(DTP)的蛋白质以赋予抗旱性的多核苷酸(WO 2012/058528);(L)赋予耐旱性和耐盐性的生育酚环化酶(TC)基因(美国专利申请公开号2012/0272352);(M)CAAX氨基末端家族蛋白质用于胁迫耐受性(美国专利号8,338,661);(N)SAL1编码基因中的突变具有增加的胁迫耐受性,包括增加的抗旱性(美国专利申请公开号2010/0257633);(O)编码增加产量相关性状的多肽(选自由GRF多肽、RAA1样多肽、SYR多肽、ARKL多肽和YTP多肽组成的组)的核酸序列的表达(美国专利申请公开号2011/0061133);(P)调节植物中编码III类海藻糖磷酸酯酶(TPP)多肽的核酸的表达,用于增强植物中产量相关的性状,特别是增加种子产量(美国专利申请公开号2010/0024067)。
影响植物生长和农艺性状(如产量、开花、植物生长和/或植物结构)的其他基因和转录因子可被引入或种质渗入到植物中,参见例如WO 1997/49811(LHY)、WO 1998/56918(ESD4)、WO 1997/10339和美国专利号6,573,430(TFL)、美国专利号6,713,663(FT)、WO1996/14414(CON)、WO 1996/38560、WO 2001/21822(VRN1)、WO 2000/44918(VRN2)、WO1999/49064(GI)、WO 2000/46358(FR1)、WO 1997/29123、美国专利号6,794,560、美国专利号6,307,126(GAI)、WO 1999/09174(D8和Rht)以及WO 2004/076638和WO 2004/031349(转录因子)。
vii.赋予增加的产量的基因
赋予增加的产量的基因的非限制性实例是:(A)由编码核酸的1-氨基环丙烷-1-羧酸酯脱氨酶样多肽(ACCDP)转化的转基因作物植物,其中,与该植物的野生型品种相比,在该作物植物中的核酸序列的表达使该植物具有增加的根生长、和/或增加的产量、和/或增加的对环境胁迫的耐受性(美国专利号8,097,769);(B)已显示,使用种子偏好性启动子的玉米锌指蛋白基因(Zm-ZFP1)的过表达能促进植物生长、增加每株植物的核数和总核重量(美国专利申请公开号2012/0079623);(C)已显示,玉米侧生器官界限(LOB)结构域蛋白(Zm-LOBDP1)的组成型过表达能增加每株植物的核数和总核重量(美国专利申请公开号2012/0079622);(D)通过调节植物中编码VIM1(甲基化1中的变体)样多肽或VTC2样(GDP-L-半乳糖磷酸化酶)多肽或DUF1685多肽或ARF6样(生长素应答因子)多肽(WO 2012/038893)的核酸的表达来增强植物中产量相关的性状;(E)调节植物中编码Ste20样多肽或其同源物的核酸的表达,得到相对于对照植物具有增加的产量的植物(EP 2431472);以及(F)编码核苷二磷酸酶激酶(NDK)多肽及其同源物的基因,用于修饰该植物根系结构(美国专利申请公开号2009/0064373)。
IX.使用方法
本文中披露的方法包含用于防治如下植物昆虫有害生物的方法:例如鞘翅目、半翅目或鳞翅目植物有害生物,包括叶甲属、瘦跗叶甲属、条跳甲属、无网蚜属、小粉虱属、茶翅蝽属、稻绿蝽属或灰翅夜蛾属植物有害生物。在一个实施例中,该方法包括向植物昆虫有害生物饲喂或施用包含本文披露的沉默元件的组合物,其中所述沉默元件在被植物昆虫有害生物(即,并不限于,鞘翅目植物有害生物,包括叶甲属植物有害生物,诸如玉米根萤叶甲、北方玉米根虫、墨西哥玉米根虫、南美叶甲或斑点黄瓜甲虫)摄取或接触时,降低该有害生物的靶标多核苷酸的水平,并且因此能防治该有害生物。可以以各种方式向该有害生物饲喂沉默元件。该沉默元件可以饲喂给雄性、雌性或两性有害生物。例如,在一个实施例中,将编码沉默元件,即靶向如SEQ ID NOS.:1-53或107-254中所示一个或多个多核苷酸的沉默元件的多核苷酸引入植物中。由于植物有害生物以表达这些序列的植物或其部分为食,所以在幼虫、成虫、或者在任何或所有的发育阶段,将所述沉默元件递送到该有害生物中。在一个实施例中,本文所述的方法和组合物进一步包含含有本文披露的沉默元件的转基因植物,其中所述沉默元件在幼虫、成虫、或者在任何或所有的发育阶段均具有不育化活性。当以这种方式将沉默元件递送到植物中时,已经认识到,该沉默元件可以以构成或替代的方式被表达,其可以通过使用各种本文别处所讨论的诱导型、或组织偏好性、或受发育调节的启动予以阶段特异性方式产生。在某些实施例中,该沉默元件在根、杆或茎、叶(包括花梗、木质部和韧皮部)、果实或生殖组织、丝、花、及其中的所有部分、或其任何组合中表达。以这种方式暴露于沉默元件可能产生不育昆虫,并且因此通过竞争性交配或SIT使相反性别的昆虫不育。
在另一种方法中,将包含本文所披露的至少一种沉默元件的组合物施用于植物。在这样的实施例中,可以将沉默元件配制在农艺学上合适的和/或环境可接受的载体(优选适于在田间喷散)中。在一些实施例中,可以将靶向不同昆虫阶段、途径和性别的沉默元件组合获得不育活性和杀昆虫活性。在一个实施例中,本文披露的沉默元件可以通过桶混与杀昆虫化学物质混合。此外,载体还可以包括增加该组合物半衰期的化合物。在某些实施例中,包含该沉默元件的组合物以这样的方式配制,使得其在环境中持续一段足以允许其被递送至植物有害生物中的时间。在此类实施例中,可将该组合物施用到植物昆虫有害生物所栖息的区域。在一个实施例中,将该组合物外部施用于植物(即,通过喷洒田地)以保护植物免受有害生物的侵害。由暴露于沉默元件产生的不育昆虫可以通过竞争性交配或SIT使异性昆虫不育。
在某些实施例中,所披露的多核苷酸或构建体可以与目的多核苷酸序列的任何组合堆叠,以产生具有所需性状的植物。本文所用的性状是指从特定序列或序列组所衍生的表型。例如,本文所述的多核苷酸可以与编码具有杀有害生物和/或杀昆虫活性的多肽的任何其他多核苷酸堆叠,例如其他苏云金芽孢杆菌毒性蛋白(描述于美国专利号5,366,892;5,747,450;5,737,514;5,723,756;5,593,881;和Geiser等人,(1986)Gene[基因]48:109中)、凝集素(Van Damme等人,(1994)Plant Mol.Biol.[植物分子生物学]24:825)、果胶(pentin)(描述于美国专利号5,981,722中)等。产生的组合也可以包括目的多核苷酸中的任一种的多个拷贝。本文所述的多核苷酸还可以与任何其他基因或基因组合堆叠以产生具有多种期望的性状组合的植物,该期望的性状包括但不限于动物饲料所期望的性状,例如高油基因(例如,美国专利号6,232,529);平衡氨基酸(例如,戈多硫蛋白(hordothionin)(美国专利号5,990,389;5,885,801;5,885,802;和5,703,409);大麦高赖氨酸(Williamson等人(1987)Eur.J.Biochem.[欧洲生物化学杂志]165:99-106;和WO 98/20122)和高蛋氨酸蛋白(Pedersen等人(1986)J.Biol.Chem.[生物化学杂志]261:6279;Kirihara等人(1988)Gene[基因]71:359;和Musumura等人(1989)Plant Mol.Biol.[植物分子生物学]12:123));增加的消化性(例如,经修饰的储存蛋白(2001年11月7日提交的美国申请序列号10/053,410);和硫氧还蛋白(2001年12月3日提交的美国申请序列号10/005,429))。
披露的多核苷酸也可以与疾病或除草剂抗性所期望的性状堆叠(例如,伏马菌素解毒基因(美国专利号5,792,931);无毒性和抗病基因(Jones等人(1994)Science[科学]266:789;Martin等人(1993)Science[科学]262:1432;Mindrinos等人(1994)Cell[细胞]78:1089);引起除草剂抗性的乙酰乳酸合酶(ALS)突变体,如S4和/或Hra突变;谷氨酰胺合酶抑制剂如草丁膦或巴斯塔(例如,bar基因);和草甘膦抗性(EPSPS基因));和加工或加工产品所需的特性,例如高油(例如,美国专利号6,232,529);经修饰的油(例如,脂肪酸去饱和酶基因(美国专利号5,952,544;WO 94/11516));经修饰的淀粉(例如,ADPG焦磷酸酶(AGP酶)、淀粉合成酶(SS)、淀粉分支酶(SBE)和淀粉脱支酶(SDBE));和聚合物或生物塑料(例如,美国专利号5.602,321);β-酮硫解酶、聚羟基丁酸合酶和乙酰乙酰辅酶A还原酶(Schubert等人(1988)J.Bacteriol.[细菌学杂志]170:5837-5847)促进聚羟基链烷酸酯(PHA)的表达);以上文献的公开内容通过引用并入本文。还可以将多核苷酸与提供农艺性状(例如,雄性不育(例如,参见美国专利号5.583,210)、茎秆强度、抗旱性(例如美国专利号7,786,353)、开花时间)或转化技术性状(如细胞周期调节或基因靶向(例如,WO 99/61619、WO 00/17364和WO 99/25821))的多核苷酸组合。
这些堆叠的组合可以通过如下任何方法产生,该方法包括但不限于,通过任何常规的或TopCross方法学进行杂交育种或遗传转化。如果通过对植物进行遗传转化来堆叠这些序列(即分子堆叠物),则可以在任何时间和以任何顺序组合这些目的多核苷酸序列。例如,可以使用包含一种或多种所需性状的转基因植物作为靶标,以通过随后的转化来引入另外的性状。可以用共转化方案将这些性状与转化盒的任何组合所提供的目的多核苷酸一起引入。例如,如果要引入两个序列,则这两个序列可以包含在单独的转化盒(反式)中或包含在相同的转化盒(顺式)中。这些序列的表达可以由相同的启动子或不同的启动子驱动。在某些情况下,引入将抑制目的多核苷酸的表达的转化盒是可取的。这可以与其他抑制盒或过表达盒的任何组合进行组合以在植物中产生期望的性状组合。进一步认识到,可以使用位点特异性重组系统将多核苷酸序列堆叠在期望的基因组位置上。参见例如WO 99/25821、WO 99/25854、WO 99/25840、WO 99/25855和WO 99/25853。
X.昆虫抗性治理方法
本文中披露的方法包括用于防治如下植物昆虫有害生物(例如昆虫抗性治理)的方法:例如鞘翅目、半翅目或鳞翅目植物有害生物,包括叶甲属、瘦跗叶甲属、条跳甲属、无网蚜属、小粉虱属、茶翅蝽属、稻绿蝽属或灰翅夜蛾属植物有害生物。昆虫抗性治理(IRM)是用来描述旨在减少昆虫有害生物变得对杀有害生物剂具有抗性的潜力的实践的术语。Bt(或其他杀有害生物蛋白,化学的或生物学的)IRM的维护是非常重要的,因为昆虫抗性对将来使用研植物并入保护剂和Bt技术整体而言构成威胁。特定的IRM策略,例如高剂量/结构化庇护所策略,延迟对玉米、棉花和马铃薯中产生的特异性Bt蛋白的昆虫抗性。然而,为了确保非抗性昆虫发育并可用于与在受保护作物中产生的任何抗性有害生物交配,这样的策略导致作物的一部分容易受到一种或多种有害生物的侵害。因此,从农民/生产者的角度来看,非常希望拥有尽可能小的庇护所,但仍然能够治理昆虫抗性,从而获得最大的产量,同时仍然保持所用的有害生物防治方法(无论是Bt、化学、一些其他方法、或其组合)的功效。
通常使用的IRM策略是种植庇护所(使用非Bt/杀有害生物性状种子的总面积的一部分),因为通常认为这将通过保持昆虫易感性来延迟昆虫对杀有害生物特性的抗性的发展。用于延迟抗性的庇护所策略的理论基础取决于如下假设:昆虫抗性的频率和隐性与有害生物易感性成反比;只有当有害生物对毒素非常敏感时,抗性才是罕见且隐性的,并且反之,当有害生物不是非常敏感时,抗性会更加频繁且隐性也更小。此外,该策略假定对Bt的抗性是隐性的并且由具有两个等位基因的单一基因座赋予,所述两个等位基因产生三种基因型:易感纯合体(SS)、杂合体(RS)和抗性纯合体(RR)。还假定初始抗性等位基因频率较低,并且抗性成虫和易感成虫之间会有大量的随机交配。在理想的情况下,只有罕见的RR个体将幸免于作物产生的杀有害生物毒素。SS个体和RS个体均易受到杀有害生物毒素的影响。结构化庇护所是种植者的田地或一组田地的非Bt/杀有害生物性状部分,其提供可能随机与幸免于所述杀有害生物特性作物(可能是Bt性状作物)的罕见抗性(RR)昆虫交配的易感(SS)昆虫的产生,以产生将被所述Bt/杀有害生物性状作物杀死的易感RS杂合子。整合的庇护所是种植者的田地或一组田地的随机种植的非Bt/杀有害生物性状部分的某些部分,其提供可能随机与幸免于所述杀有害生物特性作物的罕见抗性(RR)昆虫交配的易感(SS)昆虫的产生,以产生将被所述杀有害生物性状作物杀死的易感RS杂合子。每个庇护所策略将从昆虫群体中移除抗性(R)等位基因并延迟抗性的演变。
减少对庇护所的需求的另一个策略是对具有针对靶标昆虫有害生物的不同作用模式的性状进行聚合。例如,具有堆叠在一个转基因植物中的不同作用模式的Bt毒素对庇护所的需求降低。堆叠组合中不同的作用模式也保持了每种性状的持久性,因为抗性发展到每种性状的速度较慢。
目前,庇护所的规模、安置和管理通常被认为是减轻昆虫对玉米、棉花、大豆和其他作物中产生的Bt/杀有害生物性状的抗性的庇护所策略成功的关键。由于庇护所种植区的产量下降,一些农民选择避开庇护所需求,而其他农民则不遵循规模和/或安置要求。这些问题导致没有庇护所或庇护所效果不佳,而且抗性有害生物发展的风险也相应增加。
因此,仍然需要用于治理一片有害生物抗性作物植物中的有害生物抗性的方法。提供用于保护植物,特别是玉米或其他作物植物免受有害生物的取食损害的改良方法是有用的。如果这种方法会降低对传统化学杀有害生物剂所需的施用率,并且还将限制作物种植和栽培所需的单独田间作业的数量,这将是特别有用的。另外,具有部署转基因庇护所的方法将是有用的,该方法消除了上述关于依从性的、淡化或消除许多抗性治理策略的功效的问题。
一个实施例涉及减少抗性有害生物发展的方法,该方法包括向植物提供植物保护组合物(Bt毒素、转基因杀昆虫蛋白、其他杀昆虫蛋白、化学杀昆虫剂、杀昆虫生物学昆虫病原体等),以及使该植物有害生物与沉默元件(即,属于靶向如SEQ ID NOS.:1-53或107-254中所示的一个或多个多核苷酸的沉默元件)接触,其中所述沉默元件(即,属于靶向如SEQID NOS.:1-53或107-254中所示的一个或多个多核苷酸的沉默元件)使靶标有害生物中的序列中的一个或多个的表达减少,并通过昆虫不育化或SIT来防治所述有害生物和有害生物群体。
另外的实施例涉及增加植物有害生物组合物的持久性的方法,该方法包括向植物提供植物保护组合物(Bt毒素、转基因杀昆虫蛋白、其他杀昆虫蛋白、化学杀昆虫剂、杀昆虫生物学昆虫病原体等),以及使该植物有害生物与不育化沉默元件(即,属于如SEQ ID NO.:1-53或107-254中所示一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸的沉默元件)接触,使靶标有害生物中的序列中的一个或多个的表达减少,并通过昆虫不育化或昆虫不育技术来防治所述有害生物和有害生物群体。在另一个实施例中,作为条、块种植或与所述性状种子整合的所述庇护所包含进一步包含不育化沉默元件(例如,靶向如SEQ ID NO.:1-53或107-254中所示一个或多个多核苷酸的沉默元件)的植物。
在仍另外的实施例中,可以通过施加到非庇护所植物的不育化沉默元件的存在而减少或消除所需的庇护所。在另一个实施例中,所述庇护所或非庇护所可以包括作为喷雾剂、诱饵、引诱物,或者作为不同的转基因植物的沉默元件,即,属于如SEQ ID NO.:1-53或107-254中所示一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸的沉默元件。
在另外的实施例中,向有害昆虫饲喂如下饲料,该饲料包含如SEQ ID NO.:1-53或107-254中所示一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸的沉默元件,并且所述昆虫在饲喂后第1、2、3、4、5、6、7、8、9或10天被释放到植物上。在仍另外的实施例中,所述有害昆虫是雌性有害昆虫。在又另外的实施例中,所述有害昆虫是有害昆虫,并且在幼虫或成虫阶段饲喂所述有害昆虫。昆虫不育化可能由雄性或雌性不育,不育昆虫的交配,精子量、产卵量或卵生活力降低导致。
在某些实施例中,本文披露的通过RNAi技术靶向不育基因并将编码本文披露的沉默元件的多核苷酸与杀昆虫蛋白堆叠在转基因植物中的组合物和方法,可以提供对鞘翅目的有效防治并且可能延长鞘翅目杀昆虫性状的持久性。延长的持久性可能是最小化从鞘翅目甲虫传播抗性等位基因的结果,所述鞘翅目甲虫当以表达本文披露的一种或多种杀昆虫蛋白和RNAi不育性状的堆叠的转基因根为食时能够完成其发育生命周期。
目前的IRM策略需要高剂量的Bt毒素以便使昆虫抗性发展最小化。由于植物毒性,可能难以达到所需的高剂量。借助不同的昆虫防治手段进行有害生物综合治理(IPM)可用于延迟暴露于次最优剂量的蛋白毒素(例如Bt毒素)的昆虫抗性。可以将RNAi介导的SIT作为IPM策略的一部分进行部署。
如本文所使用的,术语“杀有害生物的”用于指针对有害生物(例如CRW)的毒性作用,并且包括外部供应的杀有害生物剂和/或由作物植物产生的试剂中的任一者或两者的活性。如本文所使用的,术语“不同的杀有害生物作用模式”包括一种或多种抗性性状的杀有害生物效果,其中无论是通过转化或传统育种方法,例如将由所述作物植物产生的杀有害生物毒素与玉米根虫的肠膜中不同的结合位点(即不同的毒素受体和/或同一毒素受体上的不同位点)结合或通过RNA干扰,将所述抗性性状引入所述作物植物中。
XI.施用方法
在一个实施例中,可以将如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物直接施用于种子。例如,在本文披露的组合物和方法中使用的如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件可以在没有附加组分和不进行稀释的情况下施用。
在一个实施例中,喷雾剂、诱饵、引诱物、引诱剂和种子处理可以包含如SEQ IDNO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物。
在另一个实施例中,将如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物以合适的制剂形式施用于种子。用于处理种子的合适的制剂和方法是本领域技术人员已知的并且例如描述于以下文献中:US 4,272,417 A、US 4,245,432 A、US 4,808,430 A、US 5,876,739 A、US2003/0176428 A1、WO 2002/080675 A1、WO 2002/028186 A2。
可以将如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物转化为常规的拌种制剂(例如溶液、乳剂、悬浮剂、粉剂、泡沫剂、浆剂或其他种子包衣材料)和ULV制剂。以已知的方式,通过将如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物与常规添加剂(例如,常规增充剂以及溶剂或稀释剂、着色剂、润湿剂、分散剂、乳化剂、消泡剂、防腐剂、二次增稠剂、粘合剂、赤霉素和水)混合来制备这些制剂。
在另一个实施例中,可能存在于拌种制剂中的合适的着色剂包括常用于此类目的的所有着色剂。可以使用在水中溶解性很低的颜料和可溶于水的染料二者。可以提及的实例包括名称为罗丹明B(Rhodamine B)、C.I.颜料红112(C.I.Pigment Red 112)、和C.I.溶剂红1(C.I.Solvent Red 1)的已知着色剂。
在另一个实施例中,可存在于所述拌种制剂中的合适的润湿剂包括促进润湿且常用于活性农用化学物质的制剂中的所有物质。优选地,有可能使用烷基萘磺酸盐,如二异丙基萘磺酸盐或二异丁基萘磺酸盐。
在仍另一个实施例中,可存在于所述拌种制剂中的合适的分散剂和/或乳化剂包括常用与活性农用化学物质的制剂中的所有非离子、阴离子和阳离子分散剂。在一个实施例中,可以使用非离子或阴离子分散剂或者非离子或阴离子分散剂的混合物。在一个实施例中,非离子分散剂包括但不限于环氧乙烷-环氧丙烷嵌段聚合物、烷基酚聚乙二醇醚和三苯乙烯苯酚聚乙二醇醚,以及它们的磷酸化或硫酸化衍生物。
在仍另一个实施例中,可以存在于根据本发明使用的拌种制剂中的消泡剂包括常用于具有农用化学活性的化合物的制剂中的所有泡沫抑制性化合物,但不限于硅酮消泡剂、硬脂酸镁、硅酮乳剂、长链醇、脂肪酸及其盐,以及有机氟化合物及其混合物。
在仍另一个实施例中,可存在于所述拌种制剂中的二次增稠剂包括可用于农业化学组合物中的此类目的的所有化合物,包括但不限于纤维素衍生物、丙烯酸衍生物、多糖类(例如黄原胶或硅酸铝镁(Veegum))、改性粘土、层状硅酸盐(如绿坡缕石和膨润土)、以及精细分散的硅酸。
可存在于待根据本发明使用的拌种制剂中的合适的粘合剂包括可用于拌种的所有常规粘合剂。聚乙烯吡咯烷酮、聚乙酸乙烯酯、聚乙烯醇和侵填体(Tylose)可以作为优选的被提及。
在另一个实施例中,如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、或者包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物在第一次施用步骤中施用于土壤、在第二次施用中施用于种子、并且在第三次施用中施用于植物的叶面区域。
如本文所使用的,将如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物施用于种子、植物、或植物部分包括将所述种子、植物、或植物部分直接和/或间接与所述如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物接触。在一个实施例中,如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物可以直接作为喷雾剂、冲洗剂(rinse)或粉剂,或其任何组合来施用。
在另一个方面,如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物可以作为粉剂直接施用于植物或植物部分。如本文所使用的,粉剂是由大量非常细的颗粒组成的干燥或接近干燥的散装固体,其在振动或倾斜时可自由流动。本文披露的干燥或接近干燥的粉末组合物优选地含有低百分比的水,例如在各个方面,小于按重量计的5%、小于2.5%、或小于1%。
在另外的实施例中,可以通过本领域技术人员已知的转化技术将如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物导入细菌、酵母或真菌中,并将所述经转化的细菌、酵母或真菌施用于植物、该植物正在生长的土壤、水培介质、种子或按照如上文所述的前述施用方法中的任一种所施用的任何一项。
在一个实施例中,可通过封装技术来配制如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物,以提高稳定性。在一个实施例中,所述封装技术可以包含随时间推移定时释放的珠状聚合物。在一个实施例中,可以将所述封装的如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物以沿犁沟单独施用珠的方式施用于种子。在另一个实施例中,可以将所述封装的如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物与种子同时共同施用。
可用于包封实施例的缓释微粒的包衣剂可以是用于用其上待负载的物质对该微粒形式进行包衣的物质。一般可以使用能够形成所述负载物质难以透过的包衣的包衣剂,而没有任何特别的限制。例如,可以使用饱和度更高的脂肪酸、蜡、热塑性树脂、热固性树脂等。
有用的饱和度更高的脂肪酸的实例包括硬脂酸、硬脂酸锌、硬脂酸酰胺和亚乙基双硬脂酸酰胺;蜡的实例包括合成蜡,如聚乙烯蜡、碳蜡、赫斯特(Hoechst)蜡和脂肪酸酯;天然蜡,如巴西棕榈蜡、蜂蜡和日本蜡;以及石油蜡,如固体石蜡和凡士林。热塑性树脂的实例包括聚烯烃,例如聚乙烯、聚丙烯、聚丁烯和聚异丁烯;乙烯基聚合物,例如聚乙酸乙烯酯、聚氯乙烯、聚偏二氯乙烯、聚丙烯酸、聚甲基丙烯酸、聚丙烯酸酯和聚甲基丙烯酸酯;二烯聚合物,例如丁二烯聚合物、异戊二烯聚合物、氯丁二烯聚合物、丁二烯-苯乙烯共聚物、乙烯-丙烯-二烯共聚物、苯乙烯-异戊二烯共聚物、MMA-丁二烯共聚物和丙烯腈-丁二烯共聚物;聚烯烃共聚物,例如乙烯-丙烯共聚物、丁烯-乙烯共聚物、丁烯-丙烯共聚物、乙烯-乙酸乙烯酯共聚物、乙烯-丙烯酸共聚物、苯乙烯-丙烯酸共聚物、乙烯-甲基丙烯酸共聚物、乙烯-甲基丙烯酸酯共聚物、乙烯-一氧化碳共聚物、乙烯-乙酸乙烯酯-一氧化碳共聚物、乙烯-乙酸乙烯酯-氯乙烯共聚物和乙烯-乙酸乙烯酯-丙烯酸共聚物;以及氯乙烯共聚物,例如氯乙烯-乙酸乙烯酯共聚物和偏二氯乙烯-氯乙烯共聚物。热固性树脂的实例包括聚氨酯树脂、环氧树脂、醇酸树脂、不饱和聚酯树脂、酚醛树脂、脲-三聚氰胺树脂、尿素树脂和硅酮树脂。在以上各项中,优选的是热塑性丙烯酸酯树脂、丁二烯苯乙烯共聚物树脂、热固性聚氨酯树脂和环氧树脂,并且在所述优选的树脂中,特别优选的是热固性聚氨酯树脂。这些包衣剂可以单独使用或者两种或更多种组合使用。
在一个实施例中,可以将如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物配制成进一步包含昆虫病原体。在一个实施例中,本公开的方法和组合物涉及如下组合物,该组合物包含如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ IDNO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸序列的沉默元件、以及包含所述序列的组合物和一种或多种生物防治剂。如本文所使用的,术语“生物防治剂”(“BCA”)包括一种或多种细菌、真菌或酵母,原生动物,病毒,昆虫病原线虫和植物提取物,或由微生物产生的产物(包括蛋白质或次级代谢产物),以及具有以下特征中的一种或两种的接种剂(innoculant):(1)抑制或减少病原体、有害生物或昆虫(包括但不限于致病性真菌、细菌和线虫)以及节肢动物有害生物(如昆虫、蜘蛛纲动物、蜈蚣、双足动物)的植物侵染和/或生长,或者抑制植物病原体、有害生物或昆虫的组合的植物侵染和/或生长;(2)提高植物性能;(3)提高植物产量;(4)提高植物活力;以及(5)提高植物健康。
XII.使用Cas/CRISPR敲除靶基因
在一个实施例中,可以使用基因组编辑技术将如SEQ ID NO.:1-53或107-254中所示的一个或多个多核苷酸或其互补序列、包含如SEQ ID NO.:1-53或107-254中所示序列或其互补序列的表达构建体、或者靶向所述多核苷酸的沉默元件、以及包含所述序列的组合物引入到植物的基因组中,或者可以使用基因组编辑技术对先前导入的编码植物基因组中本文披露的沉默元件的多核苷酸进行编辑。例如,可以通过使用双链断裂技术(如TALEN、大范围核酸酶、锌指核酸酶、CRISPR-Cas等)将披露的多核苷酸引入植物基因组中期望的位置上。例如,为了位点特异性插入的目的,可以使用CRISPR-Cas系统将所披露的多核苷酸引入基因组中期望的位置上。植物基因组中期望的位置可以是任何对于插入来说期望的靶标位点,例如适于育种的基因组区域,或者可以是位于具有现有的目的性状的基因组窗口中的靶标位点。现有的目的性状可能是内生性状或先前引入的性状。
在另一个方面,在所披露的编码沉默元件的多核苷酸先前已经被引入到基因组中的情况下,可以使用基因组编辑技术来改变或修饰引入的多核苷酸序列。可以引入披露的编码沉默元件组合物的多核苷酸中的位点特异性修饰包括使用用于引入位点特异性修饰的任何方法产生的修饰,该方法包括但不限于通过使用基因修复寡核苷酸(例如美国公开2013/0019349),或通过使用双链断裂技术,如TALEN、大范围核酸酶、锌指核酸酶、CRISPR-Cas等。此类技术可用于通过在引入的多核苷酸内的核苷酸的插入、缺失或取代来修饰先前引入的多核苷酸。可替代地,可以使用双链断裂技术向引入的多核苷酸中添加另外的核苷酸序列。可以添加的另外的序列包括另外的表达元件(例如增强子序列和启动子序列)。在另一个实施例中,可以使用基因组编辑技术在植物基因组内定位紧邻本文披露的多核苷酸组合物的另外的杀昆虫活性蛋白,以产生杀昆虫活性蛋白质的分子堆叠物。“改变的靶标位点”、“改变的靶标序列”、“修饰的靶标位点”、和“修饰的靶标序列”在本文中可互换地使用,并且意指如本文披露的靶标序列,当与非改变的靶标序列相比时,该靶标序列包括至少一种改变。例如,此类“改变”包括:(i)至少一个核苷酸的替代、(ii)至少一个核苷酸的缺失、(iii)至少一个核苷酸的插入、或(iv)(i)-(iii)的任意组合。
在一个实施例中,这些方法包括产生昆虫或其群落,其中编码靶基因,这样使得其不再起作用,从而产生不育昆虫。靶基因的多核苷酸序列可以用于通过本领域技术人员已知的手段来敲除昆虫中的靶基因多核苷酸,这些手段包括但不限于TALEN、大范围核酸酶、锌指核酸酶、CRISPR-Cas等。参见Ma等人(2014),Scientific Reports[科学报告],4:4489;Daimon等人(2013),Development,Growth,and Differentiation[发育,生长和分化],56(1):14-25;以及Eggleston等人(2001)BMC Genetics[BMC遗传学],2:11。一个实施例包含具有如SEQ ID NO.:1-53中所示的一种或多种多核苷酸中的经编辑的多核苷酸的昆虫,其中所述编辑使非功能性多肽的表达降低并且通过昆虫不育化和昆虫不育技术来防治有害生物和有害生物群体。
本说明书中提到的所有出版物和专利申请都表明本发明所属领域的技术人员的水平。将所有出版物和专利申请通过引用并入本文,其程度就像明确且个别指出通过引用将每一篇个别出版物或专利申请并入一样。
虽然为了清楚理解的目的,已经通过说明和实例的方式对前述实施例进行了详细描述,但是在所附权利要求的范围内可以实施某些改变和修改。
以下实例是通过说明的方式但不是通过限制的方式来提供的。
实验
实例1:核酸序列。
本文披露的核酸序列包含以下核酸序列。某些序列是示例性的,并且使用如下所示实例2、3和6中所述的测定方法显示出其对玉米根虫具有昆虫不育化活性。此类序列或其互补序列可以用于本文上述和下述的方法中。用于制备抑制性序列的方法是本领域已知的。本文所述的DNA构建体、载体、转基因细胞、植物、种子或产物可以包含一种或多种以下核酸或氨基酸序列,或者一种或多种本披露的的序列中的一部分。靶标多核苷酸、或者其变体和片段、及其互补序列的非限制性实例列于下表1中,包括例如SEQ ID NO.:1-53或107-254、及其变体和片段、及其互补序列。本文所提及的序列(SEQ ID NO.:1-53和107-254)表包括在下文中。
表1:VgR RNAi靶标片段。
*“转录物”表示该序列是全长mRNA转录物序列。
**“ORF”表示该序列对应于可读框序列。
实例2:通过VgR dsRNA使西方玉米根虫(WCRW)成虫不育化。
使用改进的方案来制备WCRW成虫的人工饲料(Rangasamy M等人,(2012).PestManag.Sci.[有害生物管理科学]68(4):587-91;和Nowatzki TM等人,(2006)JEcon.Entomol.[经济昆虫学期刊]99(3):927-30)。设计改进的饲料用于下文所述的饲料并入生物测定法(25μl测试样品:75μl制备的饲料)中,并使用标准96孔微量滴定板进行生产。WCRW成虫在24小时内消耗了大部分的饲料,并且在2-3周的研究期间,对照死亡率仍然<15%。对于本文描述的所有生物测定,将WCRW生命阶段(成虫和幼虫)保持在标准条件下,例如,Jackson,J.J.(1986),第25-48页,Krysan J.L.和Miller T.A.(编辑):Methods forthe Study of pest Diabrotica.Springer-Verlag,New York.[有害生物叶甲的研究方法,施普林格出版社,纽约]第260页,以及Branson,T.F.等人,(1988),J.Econ.Entomal.[经济昆虫学杂志]81(1):410-414(1988)。
将来自同一批次的甲虫分为两组。第一个试验组由雄性和<10天龄(2-5天龄)的雌性(以下简称“幼龄雌性”)组成。幼龄雌性处于其产卵前期(第一次产卵前的时期)。第二组由>11天龄并经交配的雌性(以下简称“老龄雌性”)组成,并且它们处于产卵期(雌性准备好产卵)。对于幼龄雌性组,100只甲虫(50只雌性和50只雄性),和对于老龄雌性组,50只交配的雌性,被安排用于每种处理。比较以下三种处理:1)无菌DI水(对照);2)GFP dsRNA(阴性对照,基因库登录号AY233272.1;本文中的SEQ ID NO:104);和3)VgR dsRNA片段2(SEQ ID NO:4)。使用饲料结合方法进行生物测定。分别制备GFP dsRNA和VgR dsRNA的测试样品,并在96孔微量滴定板每孔中将25μl各自的样品并入75μl改进的WCRW成虫人工饲料中,以得到终浓度为100ppm。对于对照,每孔中将25μl无菌DI水并入75μl改进的WCRW成虫人工饲料中。
使用具有单一饲料丸(25μl测试样品和75μl改进的WCRW成虫人工饲料的混合物)的32细胞托盘(C-D国际公司,皮特曼,新泽西州)的单个孔限制对单个WCRW甲虫成虫的作用,持续24小时。24小时后,收集经处理的成虫,计数并转移到其各自的笼子中,并且提供标准的SCRW干成虫饲料和水源直到研究期结束(22天至25天)。
从老龄雌性组和幼龄雌性组分别暴露后24小时或7天开始每天收集WCRW卵,持续13天至14天。用产卵盘收集卵。在加工前,将收集的卵在具有受控的照明/黑暗循环(16小时光照:8小时黑暗)的热量和湿度受控的生长室(25℃,65%±5%相对湿度(RH))中孵育12天至14天。
根据给定天数获得的卵的数量,将几个小的等分的卵-琼脂悬浮液分配到孵化板(含有2%水琼脂和两层滤纸的培养皿)上进行计数和/或孵化试验。对于所述卵孵化试验,将每个含有25μl的卵-琼脂悬浮液的样品(1-6)分配到如上所述的孵化板上,并用微孔胶带固定盖子以避免幼虫逃走。在孵育之前计数每个25μl样品中的总卵数。然后将卵孵化板在具有受控的照明/黑暗循环(16小时光照:8小时黑暗)的热量和湿度受控的生长室(25℃,65%±5%RH)中孵育三天。通过计算显示幼虫羽化孔的卵的数量,在三天期间计数卵孵化。对于每种处理,分别在老龄雌性组和幼龄雌性组暴露后的第4天和第8天,抽样4只经处理的雌性和雄性甲虫(幼龄雌性组)和4只雌性(老龄雌性组)检测基因抑制。
对于dsRNA体外转录物(“IVT”)产生,使用靶标特异性正向和反向引物(参见下表2)(在每个引物的5′末端具有T7启动子序列)进行PCR。使用Ambion Megascript高产量转录试剂盒(赛默飞世尔科技公司(Thermo Fisher Scientific),格兰德岛,纽约)从PCR模板产生dsRNA样品。运行琼脂糖凝胶以检查产量和产物大小。对于实时qRTPCR测定,使用MirVanamiRNA分离试剂盒提取总RNA,用TURBO DNase试剂盒进行处理,根据制造商的说明书通过III一步qRT-PCR试剂盒和ROX进行测定(赛默飞世尔科技公司)。使用WCRW RPS 10作为参考(即本文中分别为US 2011/0054007中的SEQ ID NO:8;还有SEQID NO:102和103、ORF以及转录物),通过delta delta Ct方法得出相对表达(Livak,K.J.和T.D.Schmittgen(2001).Methods[方法]25(4):402-408)。
表2:引物序列IVT的产生。
使用这些方法获得的数据显示在图1A-1D中。具体而言,图1A示出了在13-14天内由处理组和年龄组产生的卵的总数。在图1A中所示的实验中,幼龄雌性组包含50对雄性和雌性甲虫,而老龄雌性组包含50只交配的雌性甲虫。图1A中的数据显示摄取VgR dsRNA显著减少了测试期间产生的卵的总数。图1B显示了在13-14天产卵期期间,由处理组和年龄组每只雌性/天产生的卵的平均数量。该箱线图显示了4个四分位数、平均值、和平均数的95%置信区间。数据显示,在幼龄和老龄雌性中,摄取VgR dsRNA减少了每天产生的卵的平均数量。图1C显示了各种处理对整体平均卵孵化率的影响。数据代表13-14天的卵收集期;n=6个重复/处理/天;5-45个卵/重复,取决于天数(p<0.001)。基因抑制分析如图1D所示,用于在处理幼龄组雌性和雄性昆虫后8天和处理老龄组雌性昆虫后4天在WCRW甲虫成虫上进行的分析。使用WCRW RPS10基因作为参照和未处理的老龄甲虫作为正规化子,从每种处理的4只单个昆虫中显示VgR的相对表达。该箱线图显示了处理组和年龄组的4个四分位数、平均值、中值和平均数的95%置信区间。
实例3:通过用VgR dsRNA处理3龄幼虫使WCRW不育化。
评估通过VgR dsRNA(VgR dsRNA片段2)处理幼虫对WCRW不育化的效果。使用从玉米垫收获并在标准WCRW幼虫饲料中适应24小时的3龄幼虫进行研究。使用上述饲料结合方法(25μl dsRNA和75μl人工WCRW幼虫饲料),将大约192只幼虫暴露于水和75ppm VgR dsRNA片段2(SEQ ID NO.:4)持续1天。将经处理的幼虫置于蛹化介质中15天。收集羽化的成虫,计数并转移到其各自的笼子中,并且提供具有水源的标准SCRW干成虫饲料直到研究期结束(22天-25天)。将甲虫笼子保持在室温(通常从22℃至25℃),没有RH控制。没有刻意的光照/黑暗控制,但笼子获得大致16:8黑暗和光照的条件。甲虫笼子每周清洁维修两次,并且每次这些甲虫收到新的食物和水琼脂。甲虫成虫喂养总共26天。每个笼子在10天产卵前期后收到产卵盘,并且在16天的产卵期时间内收集卵,并按照上述方法进行处理。
这个研究的代表性数据示于图2A和2B中。图2A中示出了每只雌性产卵的平均总数和每只雌性产生的有活力卵的平均数量。对每个指定的处理计数来自15至42只雌性甲虫成虫的卵。该箱线图显示了针对每种处理的4个四分位数、平均值、中值和平均数的95%置信区间。数据显示,对于暴露于VgR dsRNA的组,在整个研究期间有活力的卵的产量一直非常低。应该指出的是,用VgR dsRNA处理不影响成虫羽化,并且VgR dsRNA组中甲虫成虫的死亡率可以忽略不计。
VgR基因抑制分析的代表性数据示于图2B中。获得的数据针对10日龄(n=4)和28日龄(n=15)甲虫,分别代表处理3龄幼虫后的第40天和第58天。通过qRTPCR的相对表达的箱线图显示了在10天龄和28天龄甲虫中针对每种处理的4个四分位数、平均值、中值和平均数的95%置信区间。将数据标准化为未经处理的3龄幼虫。数据显示在两个年龄组中VgR的相对表达降低。
实例4:通过VgR dsRNA处理,WCRW不育化和基因抑制的剂量响应。
在幼龄和老龄成虫雌性中测定dsRNA处理的剂量响应效应。收集较年长雌性组(>11天龄),并使用上述饲料结合方法将其暴露于VgR dsRNA。将处理组暴露24小时。VgRdsRNA与SEQ ID NO:3互补,并且测试的浓度如下:0、0.01ppm、0.1ppm、1ppm、10ppm和75ppm。处理组由每个剂量水平约40只至48只雌性组成。暴露后24小时开始评估产卵量并持续18天。对于每种处理,用于产卵的雌性甲虫总数范围为40只至48只(第1天-第6天)和20只至28只(第7天-第18天)。按照上述方法处理和加工卵。暴露6天后,从每种处理中取回20只经处理的雌性,并用于基因抑制分析。
针对每个剂量处理组的细节(例如分析的总卵数、有活力的卵的数量、以及产卵力的净下降)在图3A中给出。收集卵并在18天的产卵期时间内进行计数。使用以下公式估算VgR dsRNA处理的雌性相对于对照(暴露于水的雌性)的产卵力的净降低(NRF):
NRF(%)=[1-(NVEt/NVEwc)]*100,
其中“NRF”代表产卵力净降低百分比;“NVEt”代表处理组中有活力卵的数量;和“NVEwc”代表水(对照)处理组中有活力卵的数量。数据显示暴露于VgR dsRNA 10天后产卵量显著降低(见图3A中的卵/雌性,第10天-第18天)。数据进一步表明,产卵量和卵生活力与VgR dsRNA剂量呈负相关。产卵力的净降低与增加的vgR dsRNA剂量呈正相关。图3B显示了针对18天的采卵期;n=1-4个重复/处理/天;5-478个卵/重复(取决于天数和卵的可用性)根据剂量的整体卵孵化率的百分比的箱线图。图3C中的数据显示,以不同剂量的VgR dsRNA处理后第6天,VgR相对表达的箱线图。图3C中的数据显示了增加剂量与VgR表达的较大降低之间的相关性。将处理组数据标准化为未处理甲虫的表达。
实例5:VgR片段的基因抑制分析。
评估了五种不同的VgR dsRNA靶标片段的作用。图4A示意性地显示了与SEQ IDNO:2比对的测试的不同片段的相对位置。测试的靶标片段如下:Frag1是VgR片段1(SEQ IDNO:3);Frag2是VgR片段2(SEQ ID NO:4);Frag3是VgR片段3(SEQ ID NO:5);Frag4是VgR片段4(SEQ ID NO:6);以及Frag5是VgR片段5(SEQ ID NO:7)。使用上述饲料结合方法和在饲料活塞中具有100ppm的VgR dsRNA的WCRW雌性甲虫测试每个VgR dsRNA片段。将这些甲虫单独处理一天,并用不含dsRNA的标准饲料再饲喂六天。然后收集单个甲虫并在液氮中快速冷冻。对于qRTPCR测定,使用下表3中指示的引物序列,针对每个处理组使用至少3只昆虫。图4B显示了,使用5′qRTPCR测定,用所指示的dsVgR片段或对照处理(即,如所示,ddH2O和dsGUS(本文中的SEQ ID NO:105))替代饲料中的VgR dsRNA)处理后第6天的相对VgR表达的箱线图。该箱线图显示了四个四分位数、平均值(水平实线)、中值(水平虚线)和平均数的95%置信区间。用Mid-和3′-qRTPCR测定也得到类似的结果。将处理组中的数据标准化为从来自未处理的3龄幼虫的qRTPCR获得的数据。
表3:引物序列基因抑制分析。
实例6:通过基因抑制分析进行WCRW VgR片段筛选。
评估覆盖整个SEQ ID NO:2的编码DNA序列的另外的VgR dsRNA片段抑制VgR表达的能力。图5A显示了与SEQ ID NO:2比对的测试的片段,并且各种序列名称对应于表1所示的片段ID。使用上述饲料结合方法和在饲料活塞中具有100ppm的VgR dsRNA的WCRW雌性甲虫测试每个VgR dsRNA片段。将这些甲虫单独处理一天,并用不含dsRNA的标准饲料再饲喂六天。然后收集单个甲虫并在液氮中快速冷冻。对于qRTPCR测定,针对每个处理组使用至少6只昆虫。图5B-5D使用5′qRTPCR测定,用所指示的dsVgR片段处理或对照处理(即,如所示,ddH2O和dsGUS替代饲料中的VgR dsRNA)后第6天的相对VgR表达的箱线图。将处理组中的数据标准化为从来自水处理的甲虫的qRTPCR获得的数据。使用两种qRTPCR测定(5′-和Mid-qRTPCR测定)来避免VgR片段和PCR扩增子的重叠。
实例7:农杆菌介导的玉米的转化
对于用本发明的沉默元件对玉米进行的农杆菌介导的转化,采用了Zhao的方法(美国专利号5,981,840,和PCT专利公开WO 98/32326;其内容通过引用并入本文)。例如,构建体可以例如表达表1中所示的靶标序列的长双链RNA。这样的构建体可以与启动子连接。简言之,从玉米中分离未成熟的胚胎,并将胚胎与农杆菌的悬浮液接触,其中该细菌能够将包含沉默元件的多核苷酸转移至这些未成熟的胚胎中的至少一个的至少一个细胞中(步骤1:侵染步骤)。在该步骤中,将未成熟胚胎浸泡在农杆菌悬液中,引发接种。使这些胚胎与农杆菌共培养一段时间(步骤2:共培养步骤)。在该侵染步骤之后,将这些未成熟胚胎在固体培养基上进行培养。在此共同培养期之后,设想任选的“静置”步骤。在该静置步骤中,将这些胚胎在已知抑制农杆菌生长的至少一种抗生素的存在下孵育,而不添加植物转化子的选择剂(步骤3:静置步骤)。将这些未成熟的胚胎在有抗生素但没有选择剂的固体培养基上培养,以消除农杆菌并用于经侵染的细胞的静置期。接着,在含有选择剂的培养基上培养经接种的胚胎,并且回收生长的经转化的愈伤组织(步骤4:选择步骤)。在含选择剂的固体培养基上培养这些未成熟胚胎,使经转化的细胞选择性生长。然后将愈伤组织再生成植物(步骤5:再生步骤),并将生长在选择培养基上的愈伤组织在固体培养基上培养以再生植物。
实例8:WCRW VgR转基因饲喂生物测定。
使用上文所述的方法产生针对VgR Frag1、Frag2和Frag3的转基因玉米植物,并且在成虫饲喂生物测定中用于如上所述的WCRW甲虫中的基因抑制分析。使用体外转录(IVT)产物作为对照,在叶子样品中测定植物VgR片段的表达水平。根据制造商的说明书进行表达分析(Quantigene 2.0测定,昂飞公司(Affymetrix),圣克拉拉,CA 95051)。叶中的平均VgR片段表达水平(pg VgR片段/mg新鲜植物重量)显示在图6A和6B中的每个图下面。图6A显示了从针对非转基因对照(NTG)植物或表达所指示片段VgR Frag1(SEQ ID NO:3)、Frag2(SEQID NO:4)和Frag3(SEQ ID NO:5)的指示的转基因植物在约V4生长阶段从三个幼株获得的数据。试验植物在笼中被至少14只幼龄雌性甲虫侵染。饲喂后第8天收集甲虫并用于基因抑制分析。将数据标准化为在暴露于甲虫的NTG植物中获得的表达水平。图6B显示了使用非转基因对照植物或表达所指示VgR dsRNA片段的转基因植物获得的结果。单个R1玉米植物在笼中被至少6只幼龄雌性甲虫侵染。饲喂后第12天收集甲虫用于VgR表达分析。每个片段和对照由2个用于饲喂的植物和超过12只用于基因抑制分析的昆虫代表,并且将数据标准化为从暴露于类似条件的非转基因对照植物获得的数据。
实例9:WCRW成虫VeR转基因暴露生物测定。
将至少32对新羽化的甲虫成虫暴露于T1转基因转殖项或非转基因(NTG)对照植物的地上植物部分8天。重新收集甲虫,并且在笼子中安排至少13只-37只雌性甲虫用于每种处理,并保持15天以进行产卵力评估。转基因构建体表达VgR Frag1(SEQ ID NO:3)、Frag2(SEQ ID NO:4)或Frag3(SEQ ID NO:5)。对于每个构建体,测试2个至4个转殖项(图7)。每个笼子每天和/或以2天至4天的间隔接受产卵盘,并按照实例2所述的方法加工卵。
实例10:WCRW幼虫VgR转基因暴露生物测定。
将表达沉默元件(靶向SEQ ID NO:3、4和5)的玉米T1植物从培养板移植到含有Fafard Superfine盆栽混合土的温室平板中。将三株阳性单株植物(属于同一转殖项)移植并保持在温室(80°F,15小时光照:9小时黑暗)中并根据需要浇水。当植物达到V2叶子阶段时,用200个非滞育中的玉米根萤叶甲的卵对每个盆进行侵染。每天监测植物,以观察首次出现甲虫。在温室中以与以下文献中所述的相似的方式确定产生自每个盆的玉米根萤叶甲成虫的数量:Meihls等人,(2008)PNAS 105:19177-19182。每隔2天或3天如实例9中那样收集从T1转基因转殖项和非转基因(NTG)对照植物羽化的甲虫成虫,并喂养15天用于产卵力评估。针对每个转殖项,安排包含至少8对至14对雄性和雌性甲虫的三个重复笼子。每个笼子每5天接受产卵盘,并按照实例2所述的方法加工卵(图8)。
实例11:通过靶向BOULE的dsRNA处理,暴露的WCRW成虫的不育化生物测定和基因
抑制。
通过在含有蛹化介质的50mL福尔肯(Falcon)管中单独饲养3龄幼虫获得未交配过的甲虫成虫。甲虫在羽化后确定性别;饥饿24小时并使用饲料并入法暴露在100ppm的靶向BOULE靶基因(DV-BOULE-FRAG1,SEQ ID NO.:164)的dsRNA或对照(无菌水或GUS dsRNA)中一天。向经处理的甲虫提供未经处理的饲料,并再单独喂养5天。在液氮中收集至少12只混合性别的甲虫用于基因抑制分析。针对每种处理安排至少14对(雄性和雌性)用于后续交配和产卵力评估。由于交配延迟,在羽化后第19天开始产卵,并评估产卵量,持续10天。如图9A所示,高剂量成虫暴露于靶向BOULE(BOULE dsRNA,SEQ ID NO.:164)的dsRNA不影响产卵量和卵孵化率。图9B显示了BOULE dsRNA(SEQ ID NO:164)处理后甲虫中的基因表达。如之前的实例那样进行通过qRTPCR测定的相对表达。
实例12:使幼虫暴露于表达靶向BOULE的dsRNA的T1转基因植物,进行WCRW甲虫计 数。
将使用DV-BOULE-FRAG1(SEQ ID NO:164)表达靶向BOULE的dsRNA的V2叶子阶段T1转基因转殖项的三个玉米幼苗和非转基因(NTG)对照植物在温室中每盆用200个WCR卵侵染。大约在侵染后一个月,在10天内每隔2天或3天捕获从每个盆中羽化的甲虫。显示了针对来自每个转殖项至少9个盆和10个NTG对照盆的总体和每个性别(雄性和雌性)的平均甲虫数目。该箱线图显示了四个四分位数、平均值(水平虚线)、中值(水平实线)和平均值的95%置信区间。结果表明,当与NTG植物相比时,WCRW幼虫暴露于表达DV-BOULE FRAG1(SEQ IDNO:164)dsRNA的转基因转殖项不影响成虫羽化模式(图10)。如实例8中所述使用体外转录(IVT)产物作为对照,在根部样品中测定针对每个转殖项的植物BOULE片段的平均表达水平。
实例13:使WCRW幼虫暴露于表达靶向BOULE的dsRNA的转基因T1植物,导致成虫不
育化。
将从表达DV-BOULE-FRAG1(SEQ ID NO:164)dsRNA的T1转基因转殖项和非转基因(NTG)对照植物羽化的甲虫保持25天用于产卵力评估。针对每个转殖项,安排包含至少8对至14对雄性和雌性甲虫的三个重复笼子。每个笼子每5天接受产卵盘,并按照实例2所述的方法加工卵,除了卵孵化持续时间延长至8天。图11A显示了幼虫暴露于表达DV-BOULE-FRAG1(SEQ ID NO:164)dsRNA的转基因植物对来自羽化的甲虫的每只雌性的平均产卵量和每只雌性产生的平均有活力的卵的影响。图11B显示了幼虫暴露于表达DV-BOULE-FRAG1(SEQ ID NO∶164)dsRNA的转基因植物对从羽化的甲虫获得的卵的孵化率的影响。图11C表明了幼虫暴露于表达DV-BOULE-FRAG1(SEQ ID NO:164)dsRNA的转基因植物相对于NTG对照对羽化的甲虫成虫的产卵力的净降低的影响。
实例14:暴露于1ppm的靶向MAEL、NCLB和CUL3的dsRNA,3龄WCRW不育化生物测定。
按照实例3中所述方法,将超过400只3龄幼虫暴露于1ppm的各自的dsRNA样品(DV-MAEL-FRAG1,SEQ ID NO:46;DV-NCLB-FRAG1,SEQ ID NO:45;和DV-CUL3-FRAG1,SEQ ID NO:44)中。将水和GUS处理纳入作为实验对照。将经处理的幼虫分成四组,每组含有约95只至100只经处理的幼虫,并置于蛹化介质中15天。收集蛹化的成虫,计数并转移到其各自的笼子中,并且如实例3所述进行处理。10天的产卵期后,将每个重复笼中的甲虫数量调整到至少12对至16对(雄性和雌性),并评估产卵力,持续15天。针对每种处理,设置至少2个至3个重复笼子。图12中示出了每只雌性产卵的平均总数、每只雌性产生的有活力卵的平均数量、平均卵孵化率、产卵量的平均下降以及产卵力的净降低(均相对于水对照)。
实例15:通过以50DDm的3龄不育化生物测定进行WCRW不育基因筛选。
使用3龄评估幼虫的处理对通过靶向各种目的基因(GOI)的dsRNA的WCRW不育化的效果。一次测试一组8个至14个dsRNA样品,包括水和GUS对照。使用从玉米垫收获并在标准WCRW幼虫饲料中适应24小时的10日龄3龄幼虫进行研究。使用饲料结合法用于将幼虫暴露于终浓度为50ppm的6孔板的每孔2mL中的各自的测试dsRNA样品。为每个样品准备总共三个6孔板,并将约312只幼虫暴露于水或50ppm的靶dsRNA片段(GOI)1天(约104只幼虫/板;16只至18只幼虫/孔)。暴露后,取样约10只3龄幼虫用于基因抑制分析,并将剩余的处理的幼虫置于蛹化介质中15天。收集羽化的成虫,计数并转移到其各自的笼子中,并且提供具有水源(水琼脂)的标准SCRW干成虫饲料直到研究期结束(约26天)。将甲虫笼子保持在室温(通常从22℃至25℃);16:8黑暗和照明条件下,没有相对湿度控制。在10天的产卵前期后,对于每种处理,根据甲虫密度将雄性和雌性对的数量调整到16对至20对,并且每只笼子都接受产卵盘,并在持续15天的产卵期时间内收集卵,并且按照实例2所述的方法进行加工。在羽化后第10天和第25天,对一组三只雄性和三只雌性进行取样用于基因抑制。表4显示了针对活性WCRW基因靶标的产卵量、卵孵化以及产卵量和产卵力的降低的综合总结。第1栏表示GOI(目的基因),第2栏和第3栏分别表示在15天产卵期期间的总产卵量/雌性和总的有活力卵/雌性;第4-5栏表示累积的平均卵孵化(±SEM);第6栏和第7栏表示产卵量的平均降低(%)(±SEM);第8栏和第9栏表示产卵力的平均净降低(%)(±SEM)。需要注意的是,对照(水和GUS)的值是11个独立实验的累积平均值,每个实验运行持续15天的产卵期。
表4通过以50ppm*的3龄不育化生物测定进行WCRW不育基因筛选
*第1栏表示GOI(目的基因),第2栏和第3栏分别表示在15天产卵期期间的总产卵量/雌性,以及总的有活力卵/雌性;第4-5栏表示累积的平均卵孵化(±SEM);第6栏和第7栏表示产卵量的平均降低(%)(±SEM);第8栏和第9栏表示产卵力的平均净降低(%)(±SEM)。需要注意的是,对照(水和GUS)的值是11个独立实验的累积平均值,每个实验运行持续15天的产卵期。
序列表
<110> 先锋良种国际有限公司
E. I.内穆尔杜邦公司
Hu, Xu
Kernodle, Bliss
McMahon, Mollie
Kassa, Adane
Niu, Xiping
Presnail, James
Lu, Albert
Richtman, Nina
Robeson, Jonathan
<120> 用以防治昆虫有害生物的组合物和方法
<130> 6030WOPCT
<160> 254
<170> PatentIn版本3.5
<210> 1
<211> 5612
<212> DNA
<213> 玉米根虫
<400> 1
tggtcagttc gtcctttaaa actgtgcgtg ttaaactttt tgaatatctt gtaaagtatg 60
ttaattttgt tctttgtgct ttcaacgtta ttaaatgcag tcgtttcaac gaataataat 120
gattgccctc cacatcagtt tagatgcaca aattcaaaat gtatagactt tcaacaaaga 180
tgtgatggtt ccgacaattg tggagatgga tcggatgaac tggattgtga tcataaatgt 240
catgaacatt ttttcaattg tcgaaatgga agatgtataa gcgaagcatt cctgtgtgat 300
ggtgaaaatg attgtaataa ctttttggat gaaaaagact gtaagggtca aacggtgcta 360
actctcgaaa ctgatggcca ttgcgaagat ggtcattgga agtgtgccga taaattgtgt 420
attccacttg acatggtatg taatggcgca cctgaatgtt tggatggatc agatgaaact 480
attggatgca gtaacaagat cgtttgtgat ggatttaaat gtaaaaatgg ccattgcata 540
ccaactgaat gggcttgcga cggtcttgat gattgtggtg ataaaactga tgaacaagat 600
tgcgcaaacc acgtaccact tgatcaatgc actttggata aaagaaaatt cttgtgtagt 660
aacaataaaa cttgcataga tcttcactcg gtctgtgata ataagttaga ttgtcccgat 720
tattctgatg aaggaaaaca gtgcaattcg tctgcaatat catgtaacaa taacaaatgt 780
tctcatacat gtatatcact tccaacgggt ccaaaatgtc tgtgtccgaa tggttaccat 840
acgcttgatg attcgaattg tttggatatt aatgaatgta caacttatgg aatttgtgac 900
caaaaatgta gaaatcttcc tggatcatac gaatgctact gtgatgataa gtacacactc 960
caagccgata aaaaaacatg taaagcaact ggcggaagcg gaataatgat ctttagttcc 1020
aagcaggaaa ttcgagcttt aaccttagat tctttagatt attttacagt ggctaaagat 1080
ttaaatcaag tagtgggaat agcatacgat ggacatcata tttactggac agacgtattt 1140
actggacatg aaactatatc gaggtctatt gaagatggat cggaaaggga ggtattggta 1200
acatctggac tcagtctacc agaggatcta gcgtatgatt ggttaactgg taatatttat 1260
ttcaccgatg ctctcaaaca acatgttgga gtgtgttcca atgatggtca ccattgtaca 1320
gttctagtca acaaagatat tcgaaaacca cgtgggattg ttgttaatgt ggaagctgga 1380
gatatgtatt ggtctgattg gggtgtacca gcagcaattg gatattcact tatggatgga 1440
tcccaggaca aaccgttcgt caccaataat atacactggc ccaatggttt ggctctagat 1500
caacctaact ctagacttta ttggactgat gctaaaaaga tgacactaga aagtattaat 1560
ttagacggaa ctgatcaaag gatcgttttg gaaggaatag tgaaacatcc atatgccatc 1620
gctgtgtttg aaaacaaact ttattggtca gattgggact cccataccat tcaaacttgt 1680
gataagttta atggtaaaaa tcatcataca ctcgtggaag aacataaaaa tttaatctat 1740
ggaatcagta tttttcacta tgctctcgaa aagcgtctag tgaatccttg tgagcatgct 1800
tcctgtagtg acatttgttt attgaaggct cagtcatatg cttgtgcgtg cccagagaat 1860
aaagttttgg atacggatgg tcatacgtgc aaagaagtac aaccaacgca agcacttgta 1920
ggaggaactg gacacgtgtt ggtatcaatt aaacatcaat ttctaggcaa acacgatgta 1980
acattcctac cagacctggc gaaacacgta ggaagcttag catttgatag ttataaaaac 2040
attttgtatg taagcgattt ggaaacaaaa agtatagttg cgcttaacat gaacatcgga 2100
attagcaaaa ctcttgagat cgacggtctt ggcaaagtta cttcaatgga ttttgatcca 2160
aaaagtaata atttatacat ttgcgatacg gagaggctag tcgtagaaat aattgatatg 2220
aataatctag aaagaaaaat aattgtccat gacacatttg gagaaactcc tgaaagcatt 2280
gcgttggtac cggaagatgg gatcatgttt gttagcttta aacaagggaa attgggaaat 2340
agccacatcg accgtttttt catggatggt actggaagaa ctcacgtcat cgaaaacggt 2400
ttagtgggcc cagttcgtgt agtatacgac cgcagtcttt atagaatttt ctttgccgat 2460
ctaagtactg gggtaataga atctactagc gcggccggtg acgatagaca tcattttcgt 2520
actctgacta cccatccagt aagtatagca gttttgaaag atgacctatt gtggacaaat 2580
cttgactcca aggatttata ttggtctgaa aaaagatcta gcgagtctta tgagaagaaa 2640
attacgatag gttttaaaga ggataacgtc aacatccact tagtgtcagt aacacgtaaa 2700
caattagaaa tcaatagctg tcgtgttaat aataatggat gtagtcatct ttgtcttcaa 2760
tcccataaat cgatagtttg tgcatgtcca gctggatggg agctatcggc aaatggtttt 2820
acatgtaata aaagggtaac ctgcgacaat aaagagatgc tttgtcctca ttccaatact 2880
tgcgttctga agagcttaag gtgcaatggt tttaaagact gcgcatttgg tgaagatgaa 2940
tgggactgcc aaactgtttc ccaatgttta cctggccagt ataaatgcga cgatggacag 3000
tgcatcagtg aagatttggt ctgcaatcat agttatgact gtaaagataa gtctgatgag 3060
tatggatgtg cagataaaaa caaaaaacta ggatgtcctc ccggacattt tacctgtaaa 3120
agctccgaat gcatatctga acgctttgta tgtgatgctt tccatgattg cgatgatgga 3180
tcagatgaac taaactgcga aaataatatt tgtttggaat ctcagtttag gtgtgatgtc 3240
ggaacttgca taccaaaaga ttgggaatgt gatggagaat atgactgtac tgatagttca 3300
gatgagcatt gttcttcaga agtttgtcag tctaactatt ttaagtgtga caacaacaga 3360
tgtatcgatc ctaagcttca gtgcgatggg tttgatgact gcggtgatca ctctgatgaa 3420
aaattcgaga aatgtttgca tcactcgaaa gcaccaaaat gtacattgga agaattcgcg 3480
tgtattacta atacttccat ttgtcttcca aaatcagcta aatgtaacgg aacgtcagaa 3540
tgtccgaata acgaagacga aaaggattgt tcaaaatgtg aagaagatga atttgagtgc 3600
aagcataagc attatagaga atgtattcct aggtcctgga tatgtgatgg tacagatgat 3660
tgcggtgata atagtgatga gagtatggaa acctgttctt caaaacttaa acctgcggca 3720
gataactttg ctgttactga ctcgtgtcta accggatata gatgtcatag cggtgcatgc 3780
ataaatatga cctcagtatg caataataac cacgattgtt ttgatggctc tgatgaagat 3840
ggcctctgtt cttcatcttg tgtcggtgtg aaaaatcctt gcaatcaaat atgcgtaaaa 3900
acaccatctg gaccaagatg tgaatgtaaa cctggatata agttgttagg agatggaaaa 3960
acttgtattg atgataatga atgccaaacg gatccaccaa tctgtagtca gctatgtcac 4020
aataaagaag gaggctattc ttgcgattgt tacaataatt tccttttaag ttctaataaa 4080
aaatcttgta aagctcacgg accaagaatg accatatact tagtgattta cggaaaccaa 4140
atccgtcacc taattcctaa atcaaacaca atggctgttc tttacaccaa tccaatgata 4200
aaaatatcca gcttggatac tctggttaaa ccaaaactaa tcttcttcag ttcttatgaa 4260
actcaagcta tttataaact tgataccacc accaacatga tgcactacat cagaaacgta 4320
ggtttcccta gaacaattgc agttgattgg tcaactcaaa acatttatta cttcgatact 4380
gatgttaatg gccggtccat tagtgtttgc agttttgaag aaaagtgcgc taagttgata 4440
aatattgaat cgcctcgaca tgtaactgcc ttagctgttg attccgtcaa taaattgctt 4500
ttctacgttt tgaggaattg gtgggtatta gaaacaccaa gcttcgtaat atacagtgta 4560
aacttggatg gatctaatag acaagaaata gtaaaaacaa caacgggcaa tgttgaagat 4620
ataactttcg atattaacaa gaaactttta tactatacta acaaacaaga tgaaagtata 4680
cacgaagtaa gctacaaagg aggaccggtt aaaactgtat tttcaaatat ctctcaacca 4740
gaaggactta aattttttga gaaccaatta tactatactg ttgcaactgg ttatatagtc 4800
agttgtaagc tatatggaga cagatcctgc aatcccagat ataaattaca cacgtttgcc 4860
agtggtcaat ttgttattga gcaggaatcg cttcagccta aagtagaaaa tgtttgtact 4920
ggacacaaat gtccatactt atgtgtacca gcagaatcag gatatagatg tctctgtcat 4980
aacgggaaag ttagtaatca ttcagaaatt tgtggtgaaa acgatgataa tcagtcagga 5040
aataatcaca aatttgctgt acatactaca ccaatacaga ctgcatccga taaacattca 5100
ggagcagtgg cctcagcaat attaattcca ctactattag tattccttgg agcagtattt 5160
tattatttgc taaaaaggag aggtgatact ggattaaata taagcatgcg attttataac 5220
ccactctacg gaaaacctgt ccgtgaagac caaaaacaaa tccttaaacc aggacaacac 5280
gagtacacaa atccgataat tgtggccgaa gaacatgagg accacgcgaa agatgctagc 5340
agaatgctaa acgatagttg tgtttgttaa gtttgtatct ttttgttacg tcattctttg 5400
ttttatgttt tatataatct tataaacgtt gttttaatct tttgaagtgt attaaaagca 5460
aaatgactaa ttccattgtt gaacatagca atctatatta acatcaaaat agatacaaat 5520
tattatttat ctcttatatc aaacagtaga attaaaactt aagaggaaac agtggcgatc 5580
aacaggtggc gaaggcgcgt tccaggattg cg 5612
<210> 2
<211> 5313
<212> DNA
<213> 玉米根虫
<400> 2
atgttaattt tgttctttgt gctttcaacg ttattaaatg cagtcgtttc aacgaataat 60
aatgattgcc ctccacatca gtttagatgc acaaattcaa aatgtataga ctttcaacaa 120
agatgtgatg gttccgacaa ttgtggagat ggatcggatg aactggattg tgatcataaa 180
tgtcatgaac attttttcaa ttgtcgaaat ggaagatgta taagcgaagc attcctgtgt 240
gatggtgaaa atgattgtaa taactttttg gatgaaaaag actgtaaggg tcaaacggtg 300
ctaactctcg aaactgatgg ccattgcgaa gatggtcatt ggaagtgtgc cgataaattg 360
tgtattccac ttgacatggt atgtaatggc gcacctgaat gtttggatgg atcagatgaa 420
actattggat gcagtaacaa gatcgtttgt gatggattta aatgtaaaaa tggccattgc 480
ataccaactg aatgggcttg cgacggtctt gatgattgtg gtgataaaac tgatgaacaa 540
gattgcgcaa accacgtacc acttgatcaa tgcactttgg ataaaagaaa attcttgtgt 600
agtaacaata aaacttgcat agatcttcac tcggtctgtg ataataagtt agattgtccc 660
gattattctg atgaaggaaa acagtgcaat tcgtctgcaa tatcatgtaa caataacaaa 720
tgttctcata catgtatatc acttccaacg ggtccaaaat gtctgtgtcc gaatggttac 780
catacgcttg atgattcgaa ttgtttggat attaatgaat gtacaactta tggaatttgt 840
gaccaaaaat gtagaaatct tcctggatca tacgaatgct actgtgatga taagtacaca 900
ctccaagccg ataaaaaaac atgtaaagca actggcggaa gcggaataat gatctttagt 960
tccaagcagg aaattcgagc tttaacctta gattctttag attattttac agtggctaaa 1020
gatttaaatc aagtagtggg aatagcatac gatggacatc atatttactg gacagacgta 1080
tttactggac atgaaactat atcgaggtct attgaagatg gatcggaaag ggaggtattg 1140
gtaacatctg gactcagtct accagaggat ctagcgtatg attggttaac tggtaatatt 1200
tatttcaccg atgctctcaa acaacatgtt ggagtgtgtt ccaatgatgg tcaccattgt 1260
acagttctag tcaacaaaga tattcgaaaa ccacgtggga ttgttgttaa tgtggaagct 1320
ggagatatgt attggtctga ttggggtgta ccagcagcaa ttggatattc acttatggat 1380
ggatcccagg acaaaccgtt cgtcaccaat aatatacact ggcccaatgg tttggctcta 1440
gatcaaccta actctagact ttattggact gatgctaaaa agatgacact agaaagtatt 1500
aatttagacg gaactgatca aaggatcgtt ttggaaggaa tagtgaaaca tccatatgcc 1560
atcgctgtgt ttgaaaacaa actttattgg tcagattggg actcccatac cattcaaact 1620
tgtgataagt ttaatggtaa aaatcatcat acactcgtgg aagaacataa aaatttaatc 1680
tatggaatca gtatttttca ctatgctctc gaaaagcgtc tagtgaatcc ttgtgagcat 1740
gcttcctgta gtgacatttg tttattgaag gctcagtcat atgcttgtgc gtgcccagag 1800
aataaagttt tggatacgga tggtcatacg tgcaaagaag tacaaccaac gcaagcactt 1860
gtaggaggaa ctggacacgt gttggtatca attaaacatc aatttctagg caaacacgat 1920
gtaacattcc taccagacct ggcgaaacac gtaggaagct tagcatttga tagttataaa 1980
aacattttgt atgtaagcga tttggaaaca aaaagtatag ttgcgcttaa catgaacatc 2040
ggaattagca aaactcttga gatcgacggt cttggcaaag ttacttcaat ggattttgat 2100
ccaaaaagta ataatttata catttgcgat acggagaggc tagtcgtaga aataattgat 2160
atgaataatc tagaaagaaa aataattgtc catgacacat ttggagaaac tcctgaaagc 2220
attgcgttgg taccggaaga tgggatcatg tttgttagct ttaaacaagg gaaattggga 2280
aatagccaca tcgaccgttt tttcatggat ggtactggaa gaactcacgt catcgaaaac 2340
ggtttagtgg gcccagttcg tgtagtatac gaccgcagtc tttatagaat tttctttgcc 2400
gatctaagta ctggggtaat agaatctact agcgcggccg gtgacgatag acatcatttt 2460
cgtactctga ctacccatcc agtaagtata gcagttttga aagatgacct attgtggaca 2520
aatcttgact ccaaggattt atattggtct gaaaaaagat ctagcgagtc ttatgagaag 2580
aaaattacga taggttttaa agaggataac gtcaacatcc acttagtgtc agtaacacgt 2640
aaacaattag aaatcaatag ctgtcgtgtt aataataatg gatgtagtca tctttgtctt 2700
caatcccata aatcgatagt ttgtgcatgt ccagctggat gggagctatc ggcaaatggt 2760
tttacatgta ataaaagggt aacctgcgac aataaagaga tgctttgtcc tcattccaat 2820
acttgcgttc tgaagagctt aaggtgcaat ggttttaaag actgcgcatt tggtgaagat 2880
gaatgggact gccaaactgt ttcccaatgt ttacctggcc agtataaatg cgacgatgga 2940
cagtgcatca gtgaagattt ggtctgcaat catagttatg actgtaaaga taagtctgat 3000
gagtatggat gtgcagataa aaacaaaaaa ctaggatgtc ctcccggaca ttttacctgt 3060
aaaagctccg aatgcatatc tgaacgcttt gtatgtgatg ctttccatga ttgcgatgat 3120
ggatcagatg aactaaactg cgaaaataat atttgtttgg aatctcagtt taggtgtgat 3180
gtcggaactt gcataccaaa agattgggaa tgtgatggag aatatgactg tactgatagt 3240
tcagatgagc attgttcttc agaagtttgt cagtctaact attttaagtg tgacaacaac 3300
agatgtatcg atcctaagct tcagtgcgat gggtttgatg actgcggtga tcactctgat 3360
gaaaaattcg agaaatgttt gcatcactcg aaagcaccaa aatgtacatt ggaagaattc 3420
gcgtgtatta ctaatacttc catttgtctt ccaaaatcag ctaaatgtaa cggaacgtca 3480
gaatgtccga ataacgaaga cgaaaaggat tgttcaaaat gtgaagaaga tgaatttgag 3540
tgcaagcata agcattatag agaatgtatt cctaggtcct ggatatgtga tggtacagat 3600
gattgcggtg ataatagtga tgagagtatg gaaacctgtt cttcaaaact taaacctgcg 3660
gcagataact ttgctgttac tgactcgtgt ctaaccggat atagatgtca tagcggtgca 3720
tgcataaata tgacctcagt atgcaataat aaccacgatt gttttgatgg ctctgatgaa 3780
gatggcctct gttcttcatc ttgtgtcggt gtgaaaaatc cttgcaatca aatatgcgta 3840
aaaacaccat ctggaccaag atgtgaatgt aaacctggat ataagttgtt aggagatgga 3900
aaaacttgta ttgatgataa tgaatgccaa acggatccac caatctgtag tcagctatgt 3960
cacaataaag aaggaggcta ttcttgcgat tgttacaata atttcctttt aagttctaat 4020
aaaaaatctt gtaaagctca cggaccaaga atgaccatat acttagtgat ttacggaaac 4080
caaatccgtc acctaattcc taaatcaaac acaatggctg ttctttacac caatccaatg 4140
ataaaaatat ccagcttgga tactctggtt aaaccaaaac taatcttctt cagttcttat 4200
gaaactcaag ctatttataa acttgatacc accaccaaca tgatgcacta catcagaaac 4260
gtaggtttcc ctagaacaat tgcagttgat tggtcaactc aaaacattta ttacttcgat 4320
actgatgtta atggccggtc cattagtgtt tgcagttttg aagaaaagtg cgctaagttg 4380
ataaatattg aatcgcctcg acatgtaact gccttagctg ttgattccgt caataaattg 4440
cttttctacg ttttgaggaa ttggtgggta ttagaaacac caagcttcgt aatatacagt 4500
gtaaacttgg atggatctaa tagacaagaa atagtaaaaa caacaacggg caatgttgaa 4560
gatataactt tcgatattaa caagaaactt ttatactata ctaacaaaca agatgaaagt 4620
atacacgaag taagctacaa aggaggaccg gttaaaactg tattttcaaa tatctctcaa 4680
ccagaaggac ttaaattttt tgagaaccaa ttatactata ctgttgcaac tggttatata 4740
gtcagttgta agctatatgg agacagatcc tgcaatccca gatataaatt acacacgttt 4800
gccagtggtc aatttgttat tgagcaggaa tcgcttcagc ctaaagtaga aaatgtttgt 4860
actggacaca aatgtccata cttatgtgta ccagcagaat caggatatag atgtctctgt 4920
cataacggga aagttagtaa tcattcagaa atttgtggtg aaaacgatga taatcagtca 4980
ggaaataatc acaaatttgc tgtacatact acaccaatac agactgcatc cgataaacat 5040
tcaggagcag tggcctcagc aatattaatt ccactactat tagtattcct tggagcagta 5100
ttttattatt tgctaaaaag gagaggtgat actggattaa atataagcat gcgattttat 5160
aacccactct acggaaaacc tgtccgtgaa gaccaaaaac aaatccttaa accaggacaa 5220
cacgagtaca caaatccgat aattgtggcc gaagaacatg aggaccacgc gaaagatgct 5280
agcagaatgc taaacgatag ttgtgtttgt taa 5313
<210> 3
<211> 236
<212> DNA
<213> 玉米根虫
<400> 3
tgttaatttt gttctttgtg ctttcaacgt tattaaatgc agtcgtttca acgaataata 60
atgattgccc tccacatcag tttagatgca caaattcaaa atgtatagac tttcaacaaa 120
gatgtgatgg ttccgacaat tgtggagatg gatcggatga actggattgt gatcataaat 180
gtcatgaaca ttttttcaat tgtcgaaatg gaagatgtat aagcgaagca ttcctg 236
<210> 4
<211> 155
<212> DNA
<213> 玉米根虫
<400> 4
gctttcaacg ttattaaatg cagtcgtttc aacgaataat aatgattgcc ctccacatca 60
gtttagatgc acaaattcaa aatgtataga ctttcaacaa agatgtgatg gttccgacaa 120
ttgtggagat ggatcggatg aactggattg tgatc 155
<210> 5
<211> 250
<212> DNA
<213> 玉米根虫
<400> 5
gtcaccaata atatacactg gcccaatggt ttggctctag atcaacctaa ctctagactt 60
tattggactg atgctaaaaa gatgacacta gaaagtatta atttagacgg aactgatcaa 120
aggatcgttt tggaaggaat agtgaaacat ccatatgcca tcgctgtgtt tgaaaacaaa 180
ctttattggt cagattggga ctcccatacc attcaaactt gtgataagtt taatggtaaa 240
aatcatcata 250
<210> 6
<211> 155
<212> DNA
<213> 玉米根虫
<400> 6
agaggataac gtcaacatcc acttagtgtc agtaacacgt aaacaattag aaatcaatag 60
ctgtcgtgtt aataataatg gatgtagtca tctttgtctt caatcccata aatcgatagt 120
ttgtgcatgt ccagctggat gggagctatc ggcaa 155
<210> 7
<211> 155
<212> DNA
<213> 玉米根虫
<400> 7
aatattaatt ccactactat tagtattcct tggagcagta ttttattatt tgctaaaaag 60
gagaggtgat actggattaa atataagcat gcgattttat aacccactct acggaaaacc 120
tgtccgtgaa gaccaaaaac aaatccttaa accag 155
<210> 8
<211> 400
<212> DNA
<213> 玉米根虫
<400> 8
cctgtgtgat ggtgaaaatg attgtaataa ctttttggat gaaaaagact gtaagggtca 60
aacggtgcta actctcgaaa ctgatggcca ttgcgaagat ggtcattgga agtgtgccga 120
taaattgtgt attccacttg acatggtatg taatggcgca cctgaatgtt tggatggatc 180
agatgaaact attggatgca gtaacaagat cgtttgtgat ggatttaaat gtaaaaatgg 240
ccattgcata ccaactgaat gggcttgcga cggtcttgat gattgtggtg ataaaactga 300
tgaacaagat tgcgcaaacc acgtaccact tgatcaatgc actttggata aaagaaaatt 360
cttgtgtagt aacaataaaa cttgcataga tcttcactcg 400
<210> 9
<211> 400
<212> DNA
<213> 玉米根虫
<400> 9
gtctgtgata ataagttaga ttgtcccgat tattctgatg aaggaaaaca gtgcaattcg 60
tctgcaatat catgtaacaa taacaaatgt tctcatacat gtatatcact tccaacgggt 120
ccaaaatgtc tgtgtccgaa tggttaccat acgcttgatg attcgaattg tttggatatt 180
aatgaatgta caacttatgg aatttgtgac caaaaatgta gaaatcttcc tggatcatac 240
gaatgctact gtgatgataa gtacacactc caagccgata aaaaaacatg taaagcaact 300
ggcggaagcg gaataatgat ctttagttcc aagcaggaaa ttcgagcttt aaccttagat 360
tctttagatt attttacagt ggctaaagat ttaaatcaag 400
<210> 10
<211> 368
<212> DNA
<213> 玉米根虫
<400> 10
tagtgggaat agcatacgat ggacatcata tttactggac agacgtattt actggacatg 60
aaactatatc gaggtctatt gaagatggat cggaaaggga ggtattggta acatctggac 120
tcagtctacc agaggatcta gcgtatgatt ggttaactgg taatatttat ttcaccgatg 180
ctctcaaaca acatgttgga gtgtgttcca atgatggtca ccattgtaca gttctagtca 240
acaaagatat tcgaaaacca cgtgggattg ttgttaatgt ggaagctgga gatatgtatt 300
ggtctgattg gggtgtacca gcagcaattg gatattcact tatggatgga tcccaggaca 360
aaccgttc 368
<210> 11
<211> 475
<212> DNA
<213> 玉米根虫
<400> 11
cactcgtgga agaacataaa aatttaatct atggaatcag tatttttcac tatgctctcg 60
aaaagcgtct agtgaatcct tgtgagcatg cttcctgtag tgacatttgt ttattgaagg 120
ctcagtcata tgcttgtgcg tgcccagaga ataaagtttt ggatacggat ggtcatacgt 180
gcaaagaagt acaaccaacg caagcacttg taggaggaac tggacacgtg ttggtatcaa 240
ttaaacatca atttctaggc aaacacgatg taacattcct accagacctg gcgaaacacg 300
taggaagctt agcatttgat agttataaaa acattttgta tgtaagcgat ttggaaacaa 360
aaagtatagt tgcgcttaac atgaacatcg gaattagcaa aactcttgag atcgacggtc 420
ttggcaaagt tacttcaatg gattttgatc caaaaagtaa taatttatac atttg 475
<210> 12
<211> 474
<212> DNA
<213> 玉米根虫
<400> 12
cgatacggag aggctagtcg tagaaataat tgatatgaat aatctagaaa gaaaaataat 60
tgtccatgac acatttggag aaactcctga aagcattgcg ttggtaccgg aagatgggat 120
catgtttgtt agctttaaac aagggaaatt gggaaatagc cacatcgacc gttttttcat 180
ggatggtact ggaagaactc acgtcatcga aaacggttta gtgggcccag ttcgtgtagt 240
atacgaccgc agtctttata gaattttctt tgccgatcta agtactgggg taatagaatc 300
tactagcgcg gccggtgacg atagacatca ttttcgtact ctgactaccc atccagtaag 360
tatagcagtt ttgaaagatg acctattgtg gacaaatctt gactccaagg atttatattg 420
gtctgaaaaa agatctagcg agtcttatga gaagaaaatt acgataggtt ttaa 474
<210> 13
<211> 500
<212> DNA
<213> 玉米根虫
<400> 13
atggttttac atgtaataaa agggtaacct gcgacaataa agagatgctt tgtcctcatt 60
ccaatacttg cgttctgaag agcttaaggt gcaatggttt taaagactgc gcatttggtg 120
aagatgaatg ggactgccaa actgtttccc aatgtttacc tggccagtat aaatgcgacg 180
atggacagtg catcagtgaa gatttggtct gcaatcatag ttatgactgt aaagataagt 240
ctgatgagta tggatgtgca gataaaaaca aaaaactagg atgtcctccc ggacatttta 300
cctgtaaaag ctccgaatgc atatctgaac gctttgtatg tgatgctttc catgattgcg 360
atgatggatc agatgaacta aactgcgaaa ataatatttg tttggaatct cagtttaggt 420
gtgatgtcgg aacttgcata ccaaaagatt gggaatgtga tggagaatat gactgtactg 480
atagttcaga tgagcattgt 500
<210> 14
<211> 500
<212> DNA
<213> 玉米根虫
<400> 14
tcttcagaag tttgtcagtc taactatttt aagtgtgaca acaacagatg tatcgatcct 60
aagcttcagt gcgatgggtt tgatgactgc ggtgatcact ctgatgaaaa attcgagaaa 120
tgtttgcatc actcgaaagc accaaaatgt acattggaag aattcgcgtg tattactaat 180
acttccattt gtcttccaaa atcagctaaa tgtaacggaa cgtcagaatg tccgaataac 240
gaagacgaaa aggattgttc aaaatgtgaa gaagatgaat ttgagtgcaa gcataagcat 300
tatagagaat gtattcctag gtcctggata tgtgatggta cagatgattg cggtgataat 360
agtgatgaga gtatggaaac ctgttcttca aaacttaaac ctgcggcaga taactttgct 420
gttactgact cgtgtctaac cggatataga tgtcatagcg gtgcatgcat aaatatgacc 480
tcagtatgca ataataacca 500
<210> 15
<211> 251
<212> DNA
<213> 玉米根虫
<400> 15
cgattgtttt gatggctctg atgaagatgg cctctgttct tcatcttgtg tcggtgtgaa 60
aaatccttgc aatcaaatat gcgtaaaaac accatctgga ccaagatgtg aatgtaaacc 120
tggatataag ttgttaggag atggaaaaac ttgtattgat gataatgaat gccaaacgga 180
tccaccaatc tgtagtcagc tatgtcacaa taaagaagga ggctattctt gcgattgtta 240
caataatttc c 251
<210> 16
<211> 140
<212> DNA
<213> 玉米根虫
<400> 16
aaagtataca cgaagtaagc tacaaaggag gaccggttaa aactgtattt tcaaatatct 60
ctcaaccaga aggacttaaa ttttttgaga accaattata ctatactgtt gcaactggtt 120
atatagtcag ttgtaagcta 140
<210> 17
<211> 500
<212> DNA
<213> 玉米根虫
<400> 17
tatggagaca gatcctgcaa tcccagatat aaattacaca cgtttgccag tggtcaattt 60
gttattgagc aggaatcgct tcagcctaaa gtagaaaatg tttgtactgg acacaaatgt 120
ccatacttat gtgtaccagc agaatcagga tatagatgtc tctgtcataa cgggaaagtt 180
agtaatcatt cagaaatttg tggtgaaaac gatgataatc agtcaggaaa taatcacaaa 240
tttgctgtac atactacacc aatacagact gcatccgata aacattcagg agcagtggcc 300
tcagcaatat taattccact actattagta ttccttggag cagtatttta ttatttgcta 360
aaaaggagag gtgatactgg attaaatata agcatgcgat tttataaccc actctacgga 420
aaacctgtcc gtgaagacca aaaacaaatc cttaaaccag gacaacacga gtacacaaat 480
ccgataattg tggccgaaga 500
<210> 18
<211> 488
<212> DNA
<213> 玉米根虫
<400> 18
tcttgtaaag ctcacggacc aagaatgacc atatacttag tgatttacgg aaaccaaatc 60
cgtcacctaa ttcctaaatc aaacacaatg gctgttcttt acaccaatcc aatgataaaa 120
atatccagct tggatactct ggttaaacca aaactaatct tcttcagttc ttatgaaact 180
caagctattt ataaacttga taccaccacc aacatgatgc actacatcag aaacgtaggt 240
ttccctagaa caattgcagt tgattggtca actcaaaaca tttattactt cgatactgat 300
gttaatggcc ggtccattag tgtttgcagt tttgaagaaa agtgcgctaa gttgataaat 360
attgaatcgc ctcgacatgt aactgcctta gctgttgatt ccgtcaataa attgcttttc 420
tacgttttga ggaattggtg ggtattagaa acaccaagct tcgtaatata cagtgtaaac 480
ttggatgg 488
<210> 19
<211> 5313
<212> DNA
<213> 黄瓜十一星叶甲
<400> 19
atgttaattt tgttttttgt gctttccacg ttattaaatg cagtcgtttc aacgaataac 60
aatgattgtc ttcaacatca gttcagatgc acaaattcaa aatgtataga ttttcagcaa 120
agatgtgatg gttccgataa ttgtggggat ggatcggatg aactggagtg tgatcataaa 180
tgtcatgaac attttttcga ttgtcgaaat gggagatgta taagcgaagc attcgtatgt 240
gatggtgaaa atgattgtaa taactttttg gatgaaaaag actgtaagga taaaacgcta 300
actctcgaaa ctgatcagca ttgtgaagat ggtcattgga agtgtactga tagattgtgt 360
attccaaatg actgggtgtg taatggcgta cctgaatgtt tggatggatc agatgaaact 420
attggatgta gcagcaagat cgattgtgat gggtttaaat gtaaaaatgg tcattgtata 480
ccaaatgaat ggaattgcga tggtgttgac gattgcggcg ataaaactga tgaacaagat 540
tgtgaagacc acgtgccact tgatcaatgc actttggata aaagaaaatt cttgtgtagc 600
aacaataaaa cttgcatcga tcttcactca gtctgtgata ataagtttga ttgtcccgat 660
tattctgatg aaggaaaact ttgcaattcg tctacagtat tatgtaagaa taacaaatgt 720
tcgcacacat gtatcccact tccaacgggt acaaaatgtc tgtgtccgaa tggataccat 780
acgattgatg attcgaaatg tttggatatt aatgaatgta caatttatgg tatatgcgac 840
caaaaatgtc gaaatcttcc tggatcatac gaatgctact gtgatgataa atacaaactc 900
caagctgata aaagaacttg taaagcaatt ggaggaagcg gaataatgat ctttagttcc 960
aagcaggaaa ttagagcttt aaccttggat tctttagatt attttacagt ggctaaagat 1020
ttaaaacaag tagtcggaat agcatacgat ggaaatcata tttattggac agacgtattt 1080
actggacatg aaactatatc gaggtctatt gaagatggat cacagagaga ggtattggta 1140
acatctggat taagtttacc agaggatcta gcatttgatt ggttaactca taatatttat 1200
tttaccgatg ctctgaaaca acatgttgga gtatgttcca atgatggtaa ccattgtaca 1260
gtactagtca acaaagatat tcgaaaacca cgtgggattg ttgttaatgt ggaagctgga 1320
gatatgtatt ggtctgattg gggagtacca gcagcaattg gatattcact tatggatggt 1380
tcccatgaca gaccgtttgt caccaataat atacactggc ccaatggttt ggctctagat 1440
caacctaact ctagacttta ttggactgat gccaaaaaga tgacattaga aagtattaat 1500
ttagacggaa ctgatcaaag gattgttttg gaaggaatcg tgaaacatcc ctatgccatc 1560
gctgtgtttg aaaacaaatt gtattggtca gattgggact cccatagcat tcaaacttgt 1620
gataagttcg atggtaaaaa tcaccataca ctcgtagaag aacataaaaa tctaatctat 1680
ggaatcagta tctttcacta tgctctcgaa aagcgtctag tgaatccttg tgaccatact 1740
tcctgtagtg atatttgtct attgaaggct cagtcatatg cttgtgcgtg cccggaaaat 1800
aaaattttag atacggatgg tcatacgtgc aaagaaatac aaccaacgca agcacttgtt 1860
ggaggaactg gacatgtttt agtgtcaatc aaacatcaat ttctaggcaa acacgacgta 1920
acattcctac cagacctggt gaaacacgta ggaagcttag cgtatgacag ttataaaaaa 1980
gttttgtatg ttagcgattt ggaaacaaaa agtatagttg agcttaacat ggttacaggc 2040
gttagcaaaa ctcttgatat cagcggtatt ggcagagtta cttcaatgga ttttgatcca 2100
aaaagtaata atttatacat ttgcgatacg gaaaggctgg ttgtagaaat tattgatatg 2160
aatagtcaag aaagaaaaat aattgttcac gacacttttg gagaaagtcc tgaaagcatt 2220
gctttggtac cggaagatgg tatcatgttt gtaagtttta aacaaggaaa atcaggaaat 2280
agtcacatcg atcgtttatt catggatggt accggaagaa ctcacgtcat cgaaagcgga 2340
ttagtgggcc cagtccatgt agtgtatgac cacagtcttt atagaatttt ctttgcggat 2400
ctaagtactg gtgttatcga atctacgagt gtgaccggtg acgatagaca tcattttcgt 2460
actctgacta ctcatccagt aagtttagcg gttttgaaag atgatatatt gtggacaaat 2520
attgactcca aggatttata ttggtctgaa aaaagatcta gtgagtctta tgagaagaaa 2580
attactttag gttttaaaga agataacgtt aatattcact tagtatcagt gacacataaa 2640
caattagaaa ttaatggttg ccgtgttaat aataatggat gcagtcatct ttgtcttcaa 2700
tcccacaaat caataatttg tgcatgtcca gctggatggg agctatcggc aaatggtttt 2760
acatgtaata aaaggttaac ctgcgacaat aaagagatgc tttgtcctca ttccaatact 2820
tgcattctaa agagcttaag gtgcaatggt tttaaagact gcgcatttgg tgaagatgaa 2880
tgggattgtc aagctgcttc cgaatgttta cctggtcagt ataaatgcga cgatggacag 2940
tgcatcagtg aagatttggt ctgcaatcat agttatgact gtaaagataa gtcggacgag 3000
tatggatgtg cagataaaaa gaagaaacta gggtgtcctc ccggtcattt tacctgtaag 3060
agctctgaat gtatatcaga acgattttta tgtgatggtt atcatgattg cgatgatgga 3120
tcagatgaat ttaattgcga gaataatatt tgtttggaat ctcagttcag gtgtgatgtc 3180
ggaacttgca taccaaaaga ttgggaatgc gatggagaat atgactgtac tgataattca 3240
gatgagcact gttcttcaga aggttgtcgc tccgactatt tcaagtgtga taacaacaga 3300
tgtatcgatc ctaaacttca ttgcgatggg tttgatgact gcggtgacca ctctgatgaa 3360
aaattcgagc aatgtttgca tcactcgaaa gcaccaaaat gtacattgga ggaattcgca 3420
tgcaccacta atacttccat ttgtcttcca caatcagcta aatgtaacgg aacatcagaa 3480
tgcccgaata aagaagacga aaaggattgt tcaaaatgcg aagaagatga atttgagtgt 3540
aagaataagc attacagaga atgtattcct agatcctggt tatgtgatgg cgcagatgat 3600
tgcggtgata atagtgatga gaatatggaa acatgttctt caaaactcaa aactctggga 3660
aataatactg ctgttgctga tccgtgtcta accggatata gatgtcgcag cggtgcatgc 3720
ataaatatga cttcagtatg caataataaa cacgattgtt atgatggctc tgatgaagat 3780
ggactctgtt cttcatcttg tgtcggtgta aaaaatccct gcaatcaaat atgcgtaaaa 3840
acaccatctg gaccaagatg tgaatgtaga ctgggatata aattgctagg agacggaaaa 3900
acgtgtatcg atgataatga atgccaaacg gatcccccaa tctgtagtca gttatgtcac 3960
aataaagaag gaggttattc ttgtgattgt tacaataatt tccttttaag ttctaataaa 4020
aaatcttgta aggctcacgg accacgaatg accatatact tagtgattta cggaaacgaa 4080
atccgtcaac taattcctaa atcaaataca atggctgttg tttacaccaa tccaatgata 4140
aaaatctcca gcttggatac tctgattaaa ccaaaactaa tcttctttgg ttcttatgaa 4200
actcaagcta tttataaact ggataccacc acaaacatga tgcactacat cagaaacgta 4260
ggttttccta gaaaaattgc agttgattgg tccactcaaa acatttatta cttcgatact 4320
gattataatg gccggtccat tagtgtttgc agttttgaag aaaagtgtgc taagttaata 4380
aatattgaat cgcctcgaca tgtatctgcc ttagctgttg attccgttaa taaaaaactt 4440
ttctacgttt tgaggaattg gtgggtatta gaaacaccaa gcttcgtaat atacagtgta 4500
aacttggatg gatctaatag acaagaaata gtaaaaacaa caacaggtaa tgttgaagat 4560
ataactttcg atattaacaa gaaactttta tactacacta ataaacaaga tgaaagtata 4620
tatgaagtaa actacaaagg aggacctgtt aaaactgtat tttcaaacat ctctcaacca 4680
gaaggtctta aatttttcga aaatcagtta tactatactg ttgcaactgg ttatatagtc 4740
agttgtaagc tatatggaga caaatcttgt catcccagat ataaattaca cacttttgcc 4800
agtggtcaat ttattattga gcaggaatcg cttcagccta aaaccgaaaa tgtttgtact 4860
ggacacaaat gtccatactt atgtgtacca gcagaaacag gttatagatg tctttgccat 4920
gacgggaaag ttagtaatca ttcagaaatt tgtggcgaaa atgatgataa tcagtcagga 4980
aataatcaca agtttgctgt acacactaca ccattacaaa cggcatccga taaacactct 5040
ggagcagtgg cctcagcaat attaattcca atactcttgg tattccttgg agcagtattt 5100
tattatgtgc taaaaaagaa aggtgatact ggcttaaata taagcatgag attttataac 5160
ccactttacg gaaaacctgt cgaagaccaa aaaccaatcc ttcagccagg acaacacgag 5220
tacacaaatc cgatcattgt ggcagaagaa agaagtcaag aggaccatgc gaaagatgct 5280
agcagaatgc taaacgatag ttgtgtttgt taa 5313
<210> 20
<211> 5730
<212> DNA
<213> 黄瓜十一星叶甲
<400> 20
tttggccagt tcatcagtta aaactgtgag tgctacattt tttgaatatc tcgtaaagta 60
tgttaatttt gttttttgtg ctttccacgt tattaaatgc agtcgtttca acgaataaca 120
atgattgtct tcaacatcag ttcagatgca caaattcaaa atgtatagat tttcagcaaa 180
gatgtgatgg ttccgataat tgtggggatg gatcggatga actggagtgt gatcataaat 240
gtcatgaaca ttttttcgat tgtcgaaatg ggagatgtat aagcgaagca ttcgtatgtg 300
atggtgaaaa tgattgtaat aactttttgg atgaaaaaga ctgtaaggat aaaacgctaa 360
ctctcgaaac tgatcagcat tgtgaagatg gtcattggaa gtgtactgat agattgtgta 420
ttccaaatga ctgggtgtgt aatggcgtac ctgaatgttt ggatggatca gatgaaacta 480
ttggatgtag cagcaagatc gattgtgatg ggtttaaatg taaaaatggt cattgtatac 540
caaatgaatg gaattgcgat ggtgttgacg attgcggcga taaaactgat gaacaagatt 600
gtgaagacca cgtgccactt gatcaatgca ctttggataa aagaaaattc ttgtgtagca 660
acaataaaac ttgcatcgat cttcactcag tctgtgataa taagtttgat tgtcccgatt 720
attctgatga aggaaaactt tgcaattcgt ctacagtatt atgtaagaat aacaaatgtt 780
cgcacacatg tatcccactt ccaacgggta caaaatgtct gtgtccgaat ggataccata 840
cgattgatga ttcgaaatgt ttggatatta atgaatgtac aatttatggt atatgcgacc 900
aaaaatgtcg aaatcttcct ggatcatacg aatgctactg tgatgataaa tacaaactcc 960
aagctgataa aagaacttgt aaagcaattg gaggaagcgg aataatgatc tttagttcca 1020
agcaggaaat tagagcttta accttggatt ctttagatta ttttacagtg gctaaagatt 1080
taaaacaagt agtcggaata gcatacgatg gaaatcatat ttattggaca gacgtattta 1140
ctggacatga aactatatcg aggtctattg aagatggatc acagagagag gtattggtaa 1200
catctggatt aagtttacca gaggatctag catttgattg gttaactcat aatatttatt 1260
ttaccgatgc tctgaaacaa catgttggag tatgttccaa tgatggtaac cattgtacag 1320
tactagtcaa caaagatatt cgaaaaccac gtgggattgt tgttaatgtg gaagctggag 1380
atatgtattg gtctgattgg ggagtaccag cagcaattgg atattcactt atggatggtt 1440
cccatgacag accgtttgtc accaataata tacactggcc caatggtttg gctctagatc 1500
aacctaactc tagactttat tggactgatg ccaaaaagat gacattagaa agtattaatt 1560
tagacggaac tgatcaaagg attgttttgg aaggaatcgt gaaacatccc tatgccatcg 1620
ctgtgtttga aaacaaattg tattggtcag attgggactc ccatagcatt caaacttgtg 1680
ataagttcga tggtaaaaat caccatacac tcgtagaaga acataaaaat ctaatctatg 1740
gaatcagtat ctttcactat gctctcgaaa agcgtctagt gaatccttgt gaccatactt 1800
cctgtagtga tatttgtcta ttgaaggctc agtcatatgc ttgtgcgtgc ccggaaaata 1860
aaattttaga tacggatggt catacgtgca aagaaataca accaacgcaa gcacttgttg 1920
gaggaactgg acatgtttta gtgtcaatca aacatcaatt tctaggcaaa cacgacgtaa 1980
cattcctacc agacctggtg aaacacgtag gaagcttagc gtatgacagt tataaaaaag 2040
ttttgtatgt tagcgatttg gaaacaaaaa gtatagttga gcttaacatg gttacaggcg 2100
ttagcaaaac tcttgatatc agcggtattg gcagagttac ttcaatggat tttgatccaa 2160
aaagtaataa tttatacatt tgcgatacgg aaaggctggt tgtagaaatt attgatatga 2220
atagtcaaga aagaaaaata attgttcacg acacttttgg agaaagtcct gaaagcattg 2280
ctttggtacc ggaagatggt atcatgtttg taagttttaa acaaggaaaa tcaggaaata 2340
gtcacatcga tcgtttattc atggatggta ccggaagaac tcacgtcatc gaaagcggat 2400
tagtgggccc agtccatgta gtgtatgacc acagtcttta tagaattttc tttgcggatc 2460
taagtactgg tgttatcgaa tctacgagtg tgaccggtga cgatagacat cattttcgta 2520
ctctgactac tcatccagta agtttagcgg ttttgaaaga tgatatattg tggacaaata 2580
ttgactccaa ggatttatat tggtctgaaa aaagatctag tgagtcttat gagaagaaaa 2640
ttactttagg ttttaaagaa gataacgtta atattcactt agtatcagtg acacataaac 2700
aattagaaat taatggttgc cgtgttaata ataatggatg cagtcatctt tgtcttcaat 2760
cccacaaatc aataatttgt gcatgtccag ctggatggga gctatcggca aatggtttta 2820
catgtaataa aaggttaacc tgcgacaata aagagatgct ttgtcctcat tccaatactt 2880
gcattctaaa gagcttaagg tgcaatggtt ttaaagactg cgcatttggt gaagatgaat 2940
gggattgtca agctgcttcc gaatgtttac ctggtcagta taaatgcgac gatggacagt 3000
gcatcagtga agatttggtc tgcaatcata gttatgactg taaagataag tcggacgagt 3060
atggatgtgc agataaaaag aagaaactag ggtgtcctcc cggtcatttt acctgtaaga 3120
gctctgaatg tatatcagaa cgatttttat gtgatggtta tcatgattgc gatgatggat 3180
cagatgaatt taattgcgag aataatattt gtttggaatc tcagttcagg tgtgatgtcg 3240
gaacttgcat accaaaagat tgggaatgcg atggagaata tgactgtact gataattcag 3300
atgagcactg ttcttcagaa ggttgtcgct ccgactattt caagtgtgat aacaacagat 3360
gtatcgatcc taaacttcat tgcgatgggt ttgatgactg cggtgaccac tctgatgaaa 3420
aattcgagca atgtttgcat cactcgaaag caccaaaatg tacattggag gaattcgcat 3480
gcaccactaa tacttccatt tgtcttccac aatcagctaa atgtaacgga acatcagaat 3540
gcccgaataa agaagacgaa aaggattgtt caaaatgcga agaagatgaa tttgagtgta 3600
agaataagca ttacagagaa tgtattccta gatcctggtt atgtgatggc gcagatgatt 3660
gcggtgataa tagtgatgag aatatggaaa catgttcttc aaaactcaaa actctgggaa 3720
ataatactgc tgttgctgat ccgtgtctaa ccggatatag atgtcgcagc ggtgcatgca 3780
taaatatgac ttcagtatgc aataataaac acgattgtta tgatggctct gatgaagatg 3840
gactctgttc ttcatcttgt gtcggtgtaa aaaatccctg caatcaaata tgcgtaaaaa 3900
caccatctgg accaagatgt gaatgtagac tgggatataa attgctagga gacggaaaaa 3960
cgtgtatcga tgataatgaa tgccaaacgg atcccccaat ctgtagtcag ttatgtcaca 4020
ataaagaagg aggttattct tgtgattgtt acaataattt ccttttaagt tctaataaaa 4080
aatcttgtaa ggctcacgga ccacgaatga ccatatactt agtgatttac ggaaacgaaa 4140
tccgtcaact aattcctaaa tcaaatacaa tggctgttgt ttacaccaat ccaatgataa 4200
aaatctccag cttggatact ctgattaaac caaaactaat cttctttggt tcttatgaaa 4260
ctcaagctat ttataaactg gataccacca caaacatgat gcactacatc agaaacgtag 4320
gttttcctag aaaaattgca gttgattggt ccactcaaaa catttattac ttcgatactg 4380
attataatgg ccggtccatt agtgtttgca gttttgaaga aaagtgtgct aagttaataa 4440
atattgaatc gcctcgacat gtatctgcct tagctgttga ttccgttaat aaaaaacttt 4500
tctacgtttt gaggaattgg tgggtattag aaacaccaag cttcgtaata tacagtgtaa 4560
acttggatgg atctaataga caagaaatag taaaaacaac aacaggtaat gttgaagata 4620
taactttcga tattaacaag aaacttttat actacactaa taaacaagat gaaagtatat 4680
atgaagtaaa ctacaaagga ggacctgtta aaactgtatt ttcaaacatc tctcaaccag 4740
aaggtcttaa atttttcgaa aatcagttat actatactgt tgcaactggt tatatagtca 4800
gttgtaagct atatggagac aaatcttgtc atcccagata taaattacac acttttgcca 4860
gtggtcaatt tattattgag caggaatcgc ttcagcctaa aaccgaaaat gtttgtactg 4920
gacacaaatg tccatactta tgtgtaccag cagaaacagg ttatagatgt ctttgccatg 4980
acgggaaagt tagtaatcat tcagaaattt gtggcgaaaa tgatgataat cagtcaggaa 5040
ataatcacaa gtttgctgta cacactacac cattacaaac ggcatccgat aaacactctg 5100
gagcagtggc ctcagcaata ttaattccaa tactcttggt attccttgga gcagtatttt 5160
attatgtgct aaaaaagaaa ggtgatactg gcttaaatat aagcatgaga ttttataacc 5220
cactttacgg aaaacctgtc gaagaccaaa aaccaatcct tcagccagga caacacgagt 5280
acacaaatcc gatcattgtg gcagaagaaa gaagtcaaga ggaccatgcg aaagatgcta 5340
gcagaatgct aaacgatagt tgtgtttgtt aactttgtat ctttttgtta cgtcattctt 5400
tgttttatgt tttatataat cttataaacg ttgttttaat cttttgaagt gtattaaaag 5460
taaattaatt cttacacttc acaaatatta aattcgttga aaattgaatt gttgaacata 5520
acaatctata ttatacctac atcaaaatag atacaaatga atatttatta taacgctata 5580
tcgagcagta gagtttaacc ttaataccaa taaataggtg taacgggtgc atttgacaat 5640
atcaaattat ttattataga ataggctaaa tgcataatac tttatatttt tataattata 5700
ttttattaaa attttatgaa ctttaaaaaa 5730
<210> 21
<211> 5295
<212> DNA
<213> 马铃薯甲虫
<400> 21
atgtatgttc ttttggttct gggagctgta gcatcctcat cagctttctt ggaatcgcta 60
atctccctga acgactgccc tccggctcag tttgcttgca ggaatggaaa atgcgtggat 120
aaaactttga gatgcaatgg attcaacaac tgtttggatg gatctgatga aatggattgt 180
gaattgtatt tatgcaaaga gccgagatat tatcgatgta agaataaaag atgtatcagc 240
aaattatttg tttgcgatgg agaaaatgat tgcgaggatt tttctgatga agtcgaatgt 300
gagaacttta agatgtcaga tcacgtgaac acaacatgcg aaaaaggcca ctggcagtgc 360
acagataaac tgtgcatacc aaatgaatgg gtgtgcaatg gagaatcaga ttgtttagat 420
ggatcagatg aaggaatagg gtgcagctcg aaaatagagt gcgatggatt caaatgtaaa 480
aatggtcact gtattcccaa cgaatggata tgcgacaaca acaatgactg tcacgataat 540
agtgacgagg aggattgtga aaatcacgtt cctatcaaca agtgcacact ggataatcga 600
aaatttcttt gctctaacaa taaaacctgc attgacctag ttcttacgtg cgatggccat 660
cctaactgcc cggatggatc tgacgaggga cccctttgca aatcggcagt ttcttgccaa 720
aatcacgggt gctctcacga atgtttccct ttgcctacag gaccgttatg tttatgccct 780
cctggatatc ttactgagaa tgataaacgt tgccaggata tcaacgagtg cgaacagttt 840
gatatttgtg atcaaaagtg caggaatctg ccgggatcct atgaatgtta ttgcgatcat 900
aaatatttgc ttaaagaaga taaaagaact tgcaaagctg tgggtggaga agctttaatg 960
gtatttagtt ccaaatccga aataagagca tatgccttgg aatccgagtt atattttccg 1020
atagtacaaa agttgaagca agtagtcggc gttgattatg acggccacca tatctactgg 1080
acggatattt ttgcagagca tgagagtatc gcacgaacca ctgaggatgg atcaaaacga 1140
gagattctga ttacttccgg acttggttta ccagaagatt tagctgttga ttggctcaca 1200
ggaaatattt atttcacgga tgctgaaaag gcgcatatag gcgtttgtaa cagtcatgga 1260
actcactgta ccgttttggt gaacaaagat gtcaatagac ccagaggact ggtactgaat 1320
gttgaagagg gagacatgta ctggtctgat tggggaaaac cgtccaaaat tgctcattct 1380
ctgatggatg gttcaaatga cagagcattc atatctacag atattcattg gcctaatggg 1440
ctagccctgg atcaatctaa ccaaaggctt tattggactg atgctaaaaa aatgaccctg 1500
gaaagcattc gattggacgg aacggataga aggattgtgt tggaaggggt cgtgaaacat 1560
ccttatgcca ttgcggtgtt cgaaaataaa ttgtactggt cagattggtc aactcgttcc 1620
atccaaactt gtgacaaatt tactggcaaa gatcatcaca ctctggttaa agaagataag 1680
tatatatatg gaatcagcat attccattca gctctccaca gaagacttga taatccttgt 1740
gcattgtcct actgtagcga tatttgcttg ctgaagggaa atacctacag ctgtgcctgc 1800
cctgagaata aacttctggg aagtgacaag cattcatgta aagatattgg acccacagaa 1860
acgttgatcg ctgggactca agatagttta gttcatatag agcacaaatt tttgggaaaa 1920
catgatgtca cgtatctgcc cagtatagtt aaaaaaattg gttgcttggc ttatgatagt 1980
attaataact cactactgat cagcgacttg gagttgaaaa aaatcgttaa cttggatttg 2040
gatgatggaa tcaataaagt tcttgaaatt gatggattag gaagtataac agcaatggat 2100
tatgatgcta aaggaaataa cctctatgta tgtgataggg agaaacgaac cttggaagta 2160
atcagtctca atactttgtc aaaaaaaatt cttctgaact tcatgaacgg ggaaattcca 2220
gaagctgtgg ctgtggttcc ggatgaaggg gtgatgtttg tcagtttgag agaagcagaa 2280
ggtacatttt gtcatttgga tcgcttctat atggacggaa cgggaaggac acacacctta 2340
gaagatcatc tgataggccc tatatcacta cgctatgacc atgatttgaa cagagttttc 2400
tttgctgatg ctggtactgg attgatagaa tcaactagtg ttgaaggaga cgatcgacat 2460
gatttccgaa ggctaacaac ccacccctct agcatcacaa ctcttaaaaa tgatctattc 2520
tgggtgaacg aaaactccaa gcgtttgtat tgggcagaga agaaaagcat ttcaatgtat 2580
aacagaaaaa ttaccctaga tctctccaga gaaccagaga aactccatct aatatcagta 2640
acccccagaa aaaatgtgtt gagcgcttgt tggctcaaca acaatggatg cagtcacctt 2700
tgcctcatgt cgcacaagac aacggtttgc gcttgtccaa ctggctacga actatccgac 2760
gaccacagga cttgcatccg acgcgtgcat tgcgactctt ctgaattctt ttgccctcag 2820
tctaacgtct gcgtcatgaa gagcttaaga tgcaattcac atcaggactg tgcatttgga 2880
gaagatgaac tgaactgtga cataaacaga tgcacaaccg ggttcttccg ttgcgataat 2940
ggcgattgca tcaaagaaca gctagtgtgc gaccatcatt acgactgcag ggataaatct 3000
gacgaagata attgcgctga gaagaagaaa cacgtgggat gtccgccaaa gcactttaga 3060
tgcgggaatg gagactgcat ttcagataga ttcatatgcg atggttataa agattgtgag 3120
gatagctcgg atgaaaaggg ctgcgagaag atgttctgtt cggataccga atttagatgc 3180
gattctggaa catgtattcc tagtagctgg gagtgcgacc acgaatatga ttgctcagat 3240
tcctccgacg aacatcccgg atgtgcttcc atcacctgtt ctgcaaccat gttcacttgt 3300
ggaaatggca aatgcctcga taaaagtctt gtctgcgaca aaagcgatga ctgtggcgat 3360
aattcagacg aaacgtcctg cttcaaacgc ccctcctctt gccttccgca agaattttct 3420
tgtccttcga atacctctat ctgcgtccct atgtcagcca agtgcaatgg atccgtcgag 3480
tgtcctaagg gagaagacga ggaagaatgt ggcgactgca tgccggatga gttccgctgt 3540
ctcaacaagc aatgcattgc ggcacaatgg atgtgcgatg gcatcaacga ctgtgatgac 3600
ggcagtgatg aaactgctga aggttgctcg cagaagaata aaaccacggg ggagatgtcc 3660
gtggttcata ctgcctgtga aaatggattc aggtgtaaaa atggagattg cttggatatg 3720
aaactggtgt gtaatggaaa tcacgattgt tacgatggtt ctgatgagga tggactgtgt 3780
tcaacttcct gtgaaggatc agccaatcga tgttctcaaa tatgcaataa aactccaatg 3840
ggaccgtctt gcgagtgtag atctgggttt atattgatgg gcaatggggt cagctgcatg 3900
gatttgaatg aatgccagaa tcatccacca gtgtgcagtc aaatttgcca caatcaggac 3960
ggcggtttca aatgcgattg ttatgatggc tttttactga gatccgacaa gaaatcttgc 4020
aaagcagaag gaaatccgat gtcgctcatt ttcagtgaat atgggcaaat aagggaactc 4080
tcgcaattga ctaatatctt gtctgttgta tttgccactc ctgcaccaaa aataactggc 4140
ctagacgtac ttgtcaatcc aagaaccatc tatttcagca tggaggaaac ttccacgatt 4200
cacaaaatcg atatggcatc aaaaacgaga catttcataa ggaatattgg acaacctcag 4260
gacattgctg ttgattggtc tactcacaac atctattact ataacacgga caccaactcg 4320
aaatctatca gtatttgtag tttcgaagaa aagtgcgcga agttgattga tgttgacatg 4380
cacagacaag tggcaggatt ggttgtcgac tctgtcaaca aatttttatt ttatgctttg 4440
aacagttggt acgtgttcaa ttcccccagt tacgttatct acaggtgcaa cttggatgga 4500
actgagaaga cggaaataat gaaaactaca gatgggtttg tatccgacat cacttttgac 4560
cacaacagta ggatattgta ctacatggat atgtacttgg gtcaaataaa taagataacc 4620
tatgatggta gtttcaaaac tccaatttat tccaatatca cccgatcctc agggcttaag 4680
ttcttcgaga accgattata ctattctacg aatgggggat ttgtttcagt ttgcaggtta 4740
tacggatttc tgagatgtga tagtttcagg cttcacaatg atgcaataga tttcttcact 4800
attctacaag agacgcttca gccgagtgtg ggtaatcctt gcaagaacca caattgctcc 4860
aacttatgcg ttccagctca gtttggatat aaatgtctct gccaggatgg aacgcttcta 4920
tctggaccag gaagttgtcc aattaatgag aataccccag agtccgacaa aaaatatact 4980
gttcactcgg tggcgatgac acaggctggt cctgaggagg gaagttcggg tactgtagct 5040
gttgccatca cgatacctct tctggtgata ttatttgctt tgggagccat ttactttatc 5100
aagaaaaagc atgcaggagc tttgaatgtc aggatgagat tttatactcc tcatttcgga 5160
aggaccgttc aagatgaaaa tcctattcta aagcctggtc aacatgaata cacaaaccct 5220
gttcatttta ataaagaaga taatgaatta actctagaaa cagcacctat caaactgaat 5280
gatgcctcga atgtg 5295
<210> 22
<211> 5690
<212> DNA
<213> 马铃薯甲虫
<400> 22
gctctcgtag ttagcccttc tgtgttgtga aaattgtttc attttatcag tattcatcct 60
tagaaattga tatttttttc atatggtttt caaccgagat tgattactaa atatttgaga 120
atgtatgttc ttttggttct gggagctgta gcatcctcat cagctttctt ggaatcgcta 180
atctccctga acgactgccc tccggctcag tttgcttgca ggaatggaaa atgcgtggat 240
aaaactttga gatgcaatgg attcaacaac tgtttggatg gatctgatga aatggattgt 300
gaattgtatt tatgcaaaga gccgagatat tatcgatgta agaataaaag atgtatcagc 360
aaattatttg tttgcgatgg agaaaatgat tgcgaggatt tttctgatga agtcgaatgt 420
gagaacttta agatgtcaga tcacgtgaac acaacatgcg aaaaaggcca ctggcagtgc 480
acagataaac tgtgcatacc aaatgaatgg gtgtgcaatg gagaatcaga ttgtttagat 540
ggatcagatg aaggaatagg gtgcagctcg aaaatagagt gcgatggatt caaatgtaaa 600
aatggtcact gtattcccaa cgaatggata tgcgacaaca acaatgactg tcacgataat 660
agtgacgagg aggattgtga aaatcacgtt cctatcaaca agtgcacact ggataatcga 720
aaatttcttt gctctaacaa taaaacctgc attgacctag ttcttacgtg cgatggccat 780
cctaactgcc cggatggatc tgacgaggga cccctttgca aatcggcagt ttcttgccaa 840
aatcacgggt gctctcacga atgtttccct ttgcctacag gaccgttatg tttatgccct 900
cctggatatc ttactgagaa tgataaacgt tgccaggata tcaacgagtg cgaacagttt 960
gatatttgtg atcaaaagtg caggaatctg ccgggatcct atgaatgtta ttgcgatcat 1020
aaatatttgc ttaaagaaga taaaagaact tgcaaagctg tgggtggaga agctttaatg 1080
gtatttagtt ccaaatccga aataagagca tatgccttgg aatccgagtt atattttccg 1140
atagtacaaa agttgaagca agtagtcggc gttgattatg acggccacca tatctactgg 1200
acggatattt ttgcagagca tgagagtatc gcacgaacca ctgaggatgg atcaaaacga 1260
gagattctga ttacttccgg acttggttta ccagaagatt tagctgttga ttggctcaca 1320
ggaaatattt atttcacgga tgctgaaaag gcgcatatag gcgtttgtaa cagtcatgga 1380
actcactgta ccgttttggt gaacaaagat gtcaatagac ccagaggact ggtactgaat 1440
gttgaagagg gagacatgta ctggtctgat tggggaaaac cgtccaaaat tgctcattct 1500
ctgatggatg gttcaaatga cagagcattc atatctacag atattcattg gcctaatggg 1560
ctagccctgg atcaatctaa ccaaaggctt tattggactg atgctaaaaa aatgaccctg 1620
gaaagcattc gattggacgg aacggataga aggattgtgt tggaaggggt cgtgaaacat 1680
ccttatgcca ttgcggtgtt cgaaaataaa ttgtactggt cagattggtc aactcgttcc 1740
atccaaactt gtgacaaatt tactggcaaa gatcatcaca ctctggttaa agaagataag 1800
tatatatatg gaatcagcat attccattca gctctccaca gaagacttga taatccttgt 1860
gcattgtcct actgtagcga tatttgcttg ctgaagggaa atacctacag ctgtgcctgc 1920
cctgagaata aacttctggg aagtgacaag cattcatgta aagatattgg acccacagaa 1980
acgttgatcg ctgggactca agatagttta gttcatatag agcacaaatt tttgggaaaa 2040
catgatgtca cgtatctgcc cagtatagtt aaaaaaattg gttgcttggc ttatgatagt 2100
attaataact cactactgat cagcgacttg gagttgaaaa aaatcgttaa cttggatttg 2160
gatgatggaa tcaataaagt tcttgaaatt gatggattag gaagtataac agcaatggat 2220
tatgatgcta aaggaaataa cctctatgta tgtgataggg agaaacgaac cttggaagta 2280
atcagtctca atactttgtc aaaaaaaatt cttctgaact tcatgaacgg ggaaattcca 2340
gaagctgtgg ctgtggttcc ggatgaaggg gtgatgtttg tcagtttgag agaagcagaa 2400
ggtacatttt gtcatttgga tcgcttctat atggacggaa cgggaaggac acacacctta 2460
gaagatcatc tgataggccc tatatcacta cgctatgacc atgatttgaa cagagttttc 2520
tttgctgatg ctggtactgg attgatagaa tcaactagtg ttgaaggaga cgatcgacat 2580
gatttccgaa ggctaacaac ccacccctct agcatcacaa ctcttaaaaa tgatctattc 2640
tgggtgaacg aaaactccaa gcgtttgtat tgggcagaga agaaaagcat ttcaatgtat 2700
aacagaaaaa ttaccctaga tctctccaga gaaccagaga aactccatct aatatcagta 2760
acccccagaa aaaatgtgtt gagcgcttgt tggctcaaca acaatggatg cagtcacctt 2820
tgcctcatgt cgcacaagac aacggtttgc gcttgtccaa ctggctacga actatccgac 2880
gaccacagga cttgcatccg acgcgtgcat tgcgactctt ctgaattctt ttgccctcag 2940
tctaacgtct gcgtcatgaa gagcttaaga tgcaattcac atcaggactg tgcatttgga 3000
gaagatgaac tgaactgtga cataaacaga tgcacaaccg ggttcttccg ttgcgataat 3060
ggcgattgca tcaaagaaca gctagtgtgc gaccatcatt acgactgcag ggataaatct 3120
gacgaagata attgcgctga gaagaagaaa cacgtgggat gtccgccaaa gcactttaga 3180
tgcgggaatg gagactgcat ttcagataga ttcatatgcg atggttataa agattgtgag 3240
gatagctcgg atgaaaaggg ctgcgagaag atgttctgtt cggataccga atttagatgc 3300
gattctggaa catgtattcc tagtagctgg gagtgcgacc acgaatatga ttgctcagat 3360
tcctccgacg aacatcccgg atgtgcttcc atcacctgtt ctgcaaccat gttcacttgt 3420
ggaaatggca aatgcctcga taaaagtctt gtctgcgaca aaagcgatga ctgtggcgat 3480
aattcagacg aaacgtcctg cttcaaacgc ccctcctctt gccttccgca agaattttct 3540
tgtccttcga atacctctat ctgcgtccct atgtcagcca agtgcaatgg atccgtcgag 3600
tgtcctaagg gagaagacga ggaagaatgt ggcgactgca tgccggatga gttccgctgt 3660
ctcaacaagc aatgcattgc ggcacaatgg atgtgcgatg gcatcaacga ctgtgatgac 3720
ggcagtgatg aaactgctga aggttgctcg cagaagaata aaaccacggg ggagatgtcc 3780
gtggttcata ctgcctgtga aaatggattc aggtgtaaaa atggagattg cttggatatg 3840
aaactggtgt gtaatggaaa tcacgattgt tacgatggtt ctgatgagga tggactgtgt 3900
tcaacttcct gtgaaggatc agccaatcga tgttctcaaa tatgcaataa aactccaatg 3960
ggaccgtctt gcgagtgtag atctgggttt atattgatgg gcaatggggt cagctgcatg 4020
gatttgaatg aatgccagaa tcatccacca gtgtgcagtc aaatttgcca caatcaggac 4080
ggcggtttca aatgcgattg ttatgatggc tttttactga gatccgacaa gaaatcttgc 4140
aaagcagaag gaaatccgat gtcgctcatt ttcagtgaat atgggcaaat aagggaactc 4200
tcgcaattga ctaatatctt gtctgttgta tttgccactc ctgcaccaaa aataactggc 4260
ctagacgtac ttgtcaatcc aagaaccatc tatttcagca tggaggaaac ttccacgatt 4320
cacaaaatcg atatggcatc aaaaacgaga catttcataa ggaatattgg acaacctcag 4380
gacattgctg ttgattggtc tactcacaac atctattact ataacacgga caccaactcg 4440
aaatctatca gtatttgtag tttcgaagaa aagtgcgcga agttgattga tgttgacatg 4500
cacagacaag tggcaggatt ggttgtcgac tctgtcaaca aatttttatt ttatgctttg 4560
aacagttggt acgtgttcaa ttcccccagt tacgttatct acaggtgcaa cttggatgga 4620
actgagaaga cggaaataat gaaaactaca gatgggtttg tatccgacat cacttttgac 4680
cacaacagta ggatattgta ctacatggat atgtacttgg gtcaaataaa taagataacc 4740
tatgatggta gtttcaaaac tccaatttat tccaatatca cccgatcctc agggcttaag 4800
ttcttcgaga accgattata ctattctacg aatgggggat ttgtttcagt ttgcaggtta 4860
tacggatttc tgagatgtga tagtttcagg cttcacaatg atgcaataga tttcttcact 4920
attctacaag agacgcttca gccgagtgtg ggtaatcctt gcaagaacca caattgctcc 4980
aacttatgcg ttccagctca gtttggatat aaatgtctct gccaggatgg aacgcttcta 5040
tctggaccag gaagttgtcc aattaatgag aataccccag agtccgacaa aaaatatact 5100
gttcactcgg tggcgatgac acaggctggt cctgaggagg gaagttcggg tactgtagct 5160
gttgccatca cgatacctct tctggtgata ttatttgctt tgggagccat ttactttatc 5220
aagaaaaagc atgcaggagc tttgaatgtc aggatgagat tttatactcc tcatttcgga 5280
aggaccgttc aagatgaaaa tcctattcta aagcctggtc aacatgaata cacaaaccct 5340
gttcatttta ataaagaaga taatgaatta actctagaaa cagcacctat caaactgaat 5400
gatgcctcga atgtgtgata tttaataagt ttctttgggt tttacttttt atattgttgt 5460
tttatcgttt gtataagttt tatcgatgtt aaatattgaa atgttatatt tttaccgtta 5520
tagttcataa atgaagagga atgcttcaga gtgaatattg aatttgaagt gtgatattga 5580
tagcattgtt atacatttag ctattacaca ggatgaacaa tgtacacatc ctccatatat 5640
ttattatcat tgaaaaatga ataaatcatt ttaatagtac ctactgaaaa 5690
<210> 23
<211> 4413
<212> DNA
<213> 黄曲条跳甲
<400> 23
atgttactcg tattgctcct gggagcagta acagtctctt cgtcagcagt tttcccaccc 60
aaaggaaatt gcacctccga acaattccaa tgctccgatc gaagatgcat aagcctcaaa 120
gccagatgcg acttgataat ggattgcccg gacggatcgg acgaattgga atgcgatttg 180
cagctctgcg atgagccgga gttcttccgc tgtaaaaata acaaatgcat cagtcacgat 240
ttggtctgcg ataaaatgaa cgattgcgac gacttctccg acgagttgaa ctgccaaagc 300
ttccagtctt tggtggcgtc ggcttgcgag gagggcacct ggaggtgcgc cgataaattg 360
tgcataccca acgattgggt gtgcgacggc acgccggaat gcatcgacgg atccgacgaa 420
accatcggtt gcagcaggca aatcgactgc gacgggttcc gctgcaagaa caagcattgc 480
gtgcccaaag agtgggtctg cgaccatcac aacgattgca tcgataacag cgacgaagag 540
aactgcgagg accacgtccc catcgaccag tgcacgttgg acaagcgcaa gttcctctgc 600
cacaacaacg tgacgtgcat cgatacggaa tcggtttgcg acggacacaa ccactgtccg 660
gacaattcgg acgaatcgcc caattgcaac gcgtccgcca aggcgtgccg gggcgatcac 720
aagtgctcgc acgtttgcat accgctgccc gccggcccca cgtgcctctg ccccctcggc 780
taccacacgc tcgacgagaa gaactgcgag gacatcaacg aatgcgaaac atacggtata 840
tgcgatcaaa tgtgccggaa cacgccgggc tcgtacgagt gctactgcga caaggcgtac 900
catttggcgg atgacaagaa aacctgcaag gccgatggcg aggtgggcgt catggtgttc 960
agttcgaagg atcagatcag agcggtggcg ttgaatttga ccagttacat cctcgtcgct 1020
aagaacttga agaaagtggt gggcgtcgat ttcgacggtc gcagggtcta ctggacggag 1080
atatacgggg gctacgagaa catcgcccgg agcgccgagg acgggtcgca aaaggaggtt 1140
ttagtaacgg ccggtataga tctgccggag gatttggcca tcgactggtt gacgggtaac 1200
atttatttca ccgatggcga gaagaaacac ataggagttt gctcgcccga cgggacccat 1260
tgcgctgttt taatcaataa ggacatcgcg aaacccagag gcatcgttct taacgtcttg 1320
gacggcgaca tgtattggac ggattgggaa gaaccgtcgc gaatcggtta ctcattaatg 1380
gacggatcaa atgataagcc cttcgtagaa aaagacattc attggcccaa cggtttagca 1440
ttggatcatc ctaatagcag actgtactgg acggatgcta agaagatgac gttggaaagc 1500
atccgtttag atggaactga tcgaaggata atattagaag atatcgtaaa gcatccgtac 1560
gccatagcgg tattcgaaga taaattgtac tggtcggatt gggcgacgcg caccatccag 1620
cagtgcgaca aattcgacgg gaagaatcac cggacgatga tcgaagagaa ggaactcatc 1680
tacggtatca gcatatacca tccggcgcaa cagtcgcgca gcgtaaatcc ctgcgagacg 1740
gccctgtgca gcgacctgtg cttgctgaaa ggtacatcgt acagctgcgc ttgtccgcag 1800
aacaaggtcc tacagcccga cggaatgttt tgtaaagaat tgtcgcctat agagagtctc 1860
atagccgctc atcgggacat gttggtacac atcgagcatc cgattctcgg caggcatgtt 1920
gttacagctc taccgggcgc cactggagac attgaaagct tggcattcga tagcttcaaa 1980
aacgtactgt acatcagcga tttcaagact aagagaatca ccactttgca catgaagacc 2040
ggtaccagca aagtaatgga tatacccgat ttgggtagaa tcaacgctat ggatttcgat 2100
tccaaaagca acaatcttta catctgcgat tcggtccgca aagtggtaga agtaatcagt 2160
atcaacacga tggctcggcg aattttaatt cacgacacgt taggagagta cccgcaaagt 2220
atcgcgttag ttcccgacga aggggtgatg tttataagct tcggaggcga gggacgcagc 2280
cacatcgatc gcttcttcat ggacggcacc ggccgaacgc acgccatcga caccaaactg 2340
acggggcccg tcgtgctcgc gtacgacgcc gatttgcaca gggtcttctt cgccgacgcc 2400
gccaacggcg tcatcgaatc caccagcgtc gacggcgacg atcgcatcca cttcagaacc 2460
gtcgacacgc accccaccag cctggtggtc ctcaaagacg acgtgttctg gatgaacgcc 2520
cgctcgaagc agctctactg gacgtcgaag aaagtcccct cgaactacga caagaaaatc 2580
acgctcgcct tccccgacga tccggacaag gtgcacctgg cgtcggtgac gtcgcgccgc 2640
aaggagacca acctctgtcg catcaacaac aacggttgca gccacttgtg cgtccaatcg 2700
cagaaatcga tcgtgtgccg atgtccgatc ggttgggaac tgaaacagga caatcgcacg 2760
tgcgccaagc gagtgggttg catcagcgac gaatttctgt gcgcgcagtc caacgcgtgc 2820
atcgtgaaga gcctgcgttg cgacggccgg aaggattgcc tcttcggcga agacgaggac 2880
gattgcgcgg cgacgaagac gtgcggcgac gggcagttcc gatgcgccga cggcgattgc 2940
atcgccgaag ggctggcgtg caacctgcgc tacgattgca aggataaatc ggacgagcac 3000
ggttgcggcg acgtgatgaa cggcacgcga tgcgcgcccg atcacttcgc ctgctcgaac 3060
ggcgaatgca tcagcgggca tttccactgc gacggcgtct ccgattgcac ggacaatacg 3120
gacgagttgc actgtcagac gaaggagtgc aatgtaacgc agtttaggtg cgattcgggc 3180
gcttgtattc ccaaggagtg ggagtgcgat caggattacg attgcgcgga taattccgat 3240
gaacattgcg atacggtatg cccggcgagc cacttcaaat gcgacaacgg cctgtgcgtg 3300
gataagaaat tattgtgcga cggtttcgac aattgcggcg accattccga cgagaagatt 3360
cacatgtgtc accagagggc cccgcacaat tgcaccgcct acgagacggc ctgctcgtcc 3420
aacgcttcca tttgcattcc tttgggagcg aaatgcgacg gcaaatccga ctgtcccaag 3480
cacgaggacg agatcggctg cgccatgtgc gccgacgagg atttcgaatg ccgcagcaaa 3540
cacctcaagg actgcattcc gagattgtgg ttgtgcgacg gcgagaacga ttgcggcgac 3600
aacagcgacg aggacttggt gatgtgcagt aaaaagaaca agaccacgtt cgctccggcc 3660
caacaaccct gcaccgacgg cttcagatgc gacaacggcg tttgcatcaa ctctacgttg 3720
ctctgtaacg gcaaacacga ctgctacgac ggctccgacg aaggaggctc ctgctccggg 3780
tcctgcgacg gccccaagaa tccctgcaac cacgtctgcg tcaagacgcc gaagggaccg 3840
aagtgccggt gcagaccggg ctaccggttg atgggcgacg gtaaaacgtg cgtcgacgtg 3900
aacgagtgcg aggccgatcc gccgatatgc agccaacttt gccgcaacaa agatggcggt 3960
tacacgtgcg attgtttcca aaacttttat ttgaggaaga acatgaaatc gtgcaaagcg 4020
gccggttccg acatgttgat ctacttcaac gtgaacggtg acgaaatcta cgaaatctcg 4080
ccgaagaaca attcgatgag cgtcgtccag gacgggtcga tgttgaagat ctcgagtctg 4140
gccgccgtgg tggactcgaa cgagctgttc tacagcgtac ccgacaccgg cgccgtctac 4200
aagctcgatc cggccaccaa aaccgttcgc tacatcgaga acttgggcga gcccaaactg 4260
atagcggtgg attgggcgac gcgtaatgtt tattattacg acgccgaatc ggacgcgaaa 4320
tcgattggag tgtgcaattt cgaggagaag tgcgtcaaat tgatcgacgt cgatgctcat 4380
cggcaggtgt ccgccttgac cgtggattca taa 4413
<210> 24
<211> 5529
<212> DNA
<213> 黄曲条跳甲
<400> 24
aactcgtgtt acttaccttt gaaaacgatc agtttcacgt ggcctgctcg aaataattga 60
gagaatgtta ctcgtattgc tcctgggagc agtaacagtc tcttcgtcag cagttttccc 120
acccaaagga aattgcacct ccgaacaatt ccaatgctcc gatcgaagat gcataagcct 180
caaagccaga tgcgacttga taatggattg cccggacgga tcggacgaat tggaatgcga 240
tttgcagctc tgcgatgagc cggagttctt ccgctgtaaa aataacaaat gcatcagtca 300
cgatttggtc tgcgataaaa tgaacgattg cgacgacttc tccgacgagt tgaactgcca 360
aagcttccag tctttggtgg cgtcggcttg cgaggagggc acctggaggt gcgccgataa 420
attgtgcata cccaacgatt gggtgtgcga cggcacgccg gaatgcatcg acggatccga 480
cgaaaccatc ggttgcagca ggcaaatcga ctgcgacggg ttccgctgca agaacaagca 540
ttgcgtgccc aaagagtggg tctgcgacca tcacaacgat tgcatcgata acagcgacga 600
agagaactgc gaggaccacg tccccatcga ccagtgcacg ttggacaagc gcaagttcct 660
ctgccacaac aacgtgacgt gcatcgatac ggaatcggtt tgcgacggac acaaccactg 720
tccggacaat tcggacgaat cgcccaattg caacgcgtcc gccaaggcgt gccggggcga 780
tcacaagtgc tcgcacgttt gcataccgct gcccgccggc cccacgtgcc tctgccccct 840
cggctaccac acgctcgacg agaagaactg cgaggacatc aacgaatgcg aaacatacgg 900
tatatgcgat caaatgtgcc ggaacacgcc gggctcgtac gagtgctact gcgacaaggc 960
gtaccatttg gcggatgaca agaaaacctg caaggccgat ggcgaggtgg gcgtcatggt 1020
gttcagttcg aaggatcaga tcagagcggt ggcgttgaat ttgaccagtt acatcctcgt 1080
cgctaagaac ttgaagaaag tggtgggcgt cgatttcgac ggtcgcaggg tctactggac 1140
ggagatatac gggggctacg agaacatcgc ccggagcgcc gaggacgggt cgcaaaagga 1200
ggttttagta acggccggta tagatctgcc ggaggatttg gccatcgact ggttgacggg 1260
taacatttat ttcaccgatg gcgagaagaa acacatagga gtttgctcgc ccgacgggac 1320
ccattgcgct gttttaatca ataaggacat cgcgaaaccc agaggcatcg ttcttaacgt 1380
cttggacggc gacatgtatt ggacggattg ggaagaaccg tcgcgaatcg gttactcatt 1440
aatggacgga tcaaatgata agcccttcgt agaaaaagac attcattggc ccaacggttt 1500
agcattggat catcctaata gcagactgta ctggacggat gctaagaaga tgacgttgga 1560
aagcatccgt ttagatggaa ctgatcgaag gataatatta gaagatatcg taaagcatcc 1620
gtacgccata gcggtattcg aagataaatt gtactggtcg gattgggcga cgcgcaccat 1680
ccagcagtgc gacaaattcg acgggaagaa tcaccggacg atgatcgaag agaaggaact 1740
catctacggt atcagcatat accatccggc gcaacagtcg cgcagcgtaa atccctgcga 1800
gacggccctg tgcagcgacc tgtgcttgct gaaaggtaca tcgtacagct gcgcttgtcc 1860
gcagaacaag gtcctacagc ccgacggaat gttttgtaaa gaattgtcgc ctatagagag 1920
tctcatagcc gctcatcggg acatgttggt acacatcgag catccgattc tcggcaggca 1980
tgttgttaca gctctaccgg gcgccactgg agacattgaa agcttggcat tcgatagctt 2040
caaaaacgta ctgtacatca gcgatttcaa gactaagaga atcaccactt tgcacatgaa 2100
gaccggtacc agcaaagtaa tggatatacc cgatttgggt agaatcaacg ctatggattt 2160
cgattccaaa agcaacaatc tttacatctg cgattcggtc cgcaaagtgg tagaagtaat 2220
cagtatcaac acgatggctc ggcgaatttt aattcacgac acgttaggag agtacccgca 2280
aagtatcgcg ttagttcccg acgaaggggt gatgtttata agcttcggag gcgagggacg 2340
cagccacatc gatcgcttct tcatggacgg caccggccga acgcacgcca tcgacaccaa 2400
actgacgggg cccgtcgtgc tcgcgtacga cgccgatttg cacagggtct tcttcgccga 2460
cgccgccaac ggcgtcatcg aatccaccag cgtcgacggc gacgatcgca tccacttcag 2520
aaccgtcgac acgcacccca ccagcctggt ggtcctcaaa gacgacgtgt tctggatgaa 2580
cgcccgctcg aagcagctct actggacgtc gaagaaagtc ccctcgaact acgacaagaa 2640
aatcacgctc gccttccccg acgatccgga caaggtgcac ctggcgtcgg tgacgtcgcg 2700
ccgcaaggag accaacctct gtcgcatcaa caacaacggt tgcagccact tgtgcgtcca 2760
atcgcagaaa tcgatcgtgt gccgatgtcc gatcggttgg gaactgaaac aggacaatcg 2820
cacgtgcgcc aagcgagtgg gttgcatcag cgacgaattt ctgtgcgcgc agtccaacgc 2880
gtgcatcgtg aagagcctgc gttgcgacgg ccggaaggat tgcctcttcg gcgaagacga 2940
ggacgattgc gcggcgacga agacgtgcgg cgacgggcag ttccgatgcg ccgacggcga 3000
ttgcatcgcc gaagggctgg cgtgcaacct gcgctacgat tgcaaggata aatcggacga 3060
gcacggttgc ggcgacgtga tgaacggcac gcgatgcgcg cccgatcact tcgcctgctc 3120
gaacggcgaa tgcatcagcg ggcatttcca ctgcgacggc gtctccgatt gcacggacaa 3180
tacggacgag ttgcactgtc agacgaagga gtgcaatgta acgcagttta ggtgcgattc 3240
gggcgcttgt attcccaagg agtgggagtg cgatcaggat tacgattgcg cggataattc 3300
cgatgaacat tgcgatacgg tatgcccggc gagccacttc aaatgcgaca acggcctgtg 3360
cgtggataag aaattattgt gcgacggttt cgacaattgc ggcgaccatt ccgacgagaa 3420
gattcacatg tgtcaccaga gggccccgca caattgcacc gcctacgaga cggcctgctc 3480
gtccaacgct tccatttgca ttcctttggg agcgaaatgc gacggcaaat ccgactgtcc 3540
caagcacgag gacgagatcg gctgcgccat gtgcgccgac gaggatttcg aatgccgcag 3600
caaacacctc aaggactgca ttccgagatt gtggttgtgc gacggcgaga acgattgcgg 3660
cgacaacagc gacgaggact tggtgatgtg cagtaaaaag aacaagacca cgttcgctcc 3720
ggcccaacaa ccctgcaccg acggcttcag atgcgacaac ggcgtttgca tcaactctac 3780
gttgctctgt aacggcaaac acgactgcta cgacggctcc gacgaaggag gctcctgctc 3840
cgggtcctgc gacggcccca agaatccctg caaccacgtc tgcgtcaaga cgccgaaggg 3900
accgaagtgc cggtgcagac cgggctaccg gttgatgggc gacggtaaaa cgtgcgtcga 3960
cgtgaacgag tgcgaggccg atccgccgat atgcagccaa ctttgccgca acaaagatgg 4020
cggttacacg tgcgattgtt tccaaaactt ttatttgagg aagaacatga aatcgtgcaa 4080
agcggccggt tccgacatgt tgatctactt caacgtgaac ggtgacgaaa tctacgaaat 4140
ctcgccgaag aacaattcga tgagcgtcgt ccaggacggg tcgatgttga agatctcgag 4200
tctggccgcc gtggtggact cgaacgagct gttctacagc gtacccgaca ccggcgccgt 4260
ctacaagctc gatccggcca ccaaaaccgt tcgctacatc gagaacttgg gcgagcccaa 4320
actgatagcg gtggattggg cgacgcgtaa tgtttattat tacgacgccg aatcggacgc 4380
gaaatcgatt ggagtgtgca atttcgagga gaagtgcgtc aaattgatcg acgtcgatgc 4440
tcatcggcag gtgtccgcct tgaccgtgga ttcataaatt gatcgacgtc gatgctcatc 4500
gacaggtgtc cgccttggcc gtggattcgt acaacaaggt tttgttctat gtgctgagat 4560
cgtggacgat attcgcatcg cccagttacg ttttgtacaa aaccgatttg gacggtagca 4620
acgtggtgga actgatcaaa acaaatatag gtaacgtgga acacatcacc tacgatttga 4680
acaaacgcca gttgtacttc cacgacgaaa acacccatca aatcaacacg atcggctaca 4740
acgggggcaa cgtcaaaccg ctcttctaca atgtatcatc actgatcgaa gggctcaaac 4800
tgttcgaaga caatttgtac tatctgcgcc aggacgggtt cctcattagg tgtcagttgt 4860
tcgaagaggc cgtctgtcac tacggtttca atttgcacag tctggccagg gacgatttcg 4920
tcatctcgca ccgatccctt cagcccttgg tcgaaaacgt ctgcgacggt cacagttgcc 4980
cttacatgtg cgtggccgga cgagagggct acaagtgcat ttgccacgat ggctcgacta 5040
ctggcgaatg cggtacacca caagacgaca acggtgcgaa caaattcaaa gtaaccacca 5100
ttgaaggaaa atccaattct tcggcgttct tgtcgggctt cggggtggta ctcgtgttac 5160
tcgcttgcgt catgtttata gttgggttgt attacttgct gaagaagaga agcgcagaaa 5220
acagtacagc agatttaagt attgtccgtt ttcaaaatcc gctttacgga agaccggtgg 5280
aagacgagaa accgatcttg gaacccggca agcacgagta cgtgaattgt ttgtacgaac 5340
acaaaacgga tggcggcgaa aacagcaatg cggaacccaa aacgtttaac gtctgtgatg 5400
ataatttgtg ttaatttatt gtttttttta tgttttacgt taattaagtg cattatttga 5460
ttgttaattc tatttataac acattattat gtatatacaa aatttgttaa atatttaata 5520
aatatagag 5529
<210> 25
<211> 5226
<212> DNA
<213> 茶翅蝽
<400> 25
atgctgtcgc cacgatatag gttaaaattt tccaacgatt tgaagttttt gtttgcgact 60
atattaatcg ctttggtcac ttctactgaa cagcatgata cagattgtga tgaaaatttc 120
ttcagatgcc gcaatggtag atgtatatct ctcagcttta tttgcgatgg ttacgaagac 180
tgcggccatg gggacatgtc tgatgaagaa aattgccatt tgaccactac cccagcagtg 240
aaaccgtgca ataaagatga atatcagtgc aaagataggc tgtgcattcc ttcgttatgg 300
gtctgcgatg gtgaatcaga ctgttcggat ggcagcgatg agactcatgg atgtagtgag 360
gcgacctgtg acggttttca gtgccacagc aaatcttgta tccccaaaga gtgggagtgc 420
gatggcgtac gtgattgtcc agataactca gacgaacagt tctgtgctaa gaaaccagtg 480
agcactgaag aatgcaatat tcatgaaaat ggtttcatgt gccatgatag tatgaaatgc 540
attgaactgg aagaagtctg caacagtcac cctgattgcg atgatggttc tgatgaaggt 600
ggtatgtgcc tcaatccgaa caaaacagat ttatgctcta atctgaaatg tcccacaaat 660
caaggctgta taatattacc cgagggcccg gtatgtactg aggtgtgcaa gaaaggatac 720
cacttcgcca gtggttcttg ttttgatatc gatgaatgta taacatttgg aaggtgtgat 780
caaatatgta taaacacaaa tggaggctac aattgctcct gcattgaagg ctatagattt 840
gataatgatt tgaaaaaatg caaagctaga ggaagtgaag gtatactatt tttcacttca 900
aatcaagaaa taagaggata ttatttagac tccgaaatct actttaaagt agttgaaaat 960
ctaccacatg caacaggagt agcatatgat ggtgttaatg tttactggac aacagtagca 1020
gatgaggaag aaacaattgt caaagcagct gaaaatggtg aaaatcagaa aataattgta 1080
acttctggta ttggcagtcc tgaagatcta gcagttgatt gggttacaaa aaatgtatac 1140
ttcacggatt ctaaattaaa gtatgtaggt gtttgttcca atgatggata ctattgcctt 1200
atactccatg aagatcaagt tgagaaacca cgtgctattg ttctgcattc atcagaggga 1260
ctgatgtatt ggtctgactg gggaacagaa cctgccatta tgcgttctgg aatggatgga 1320
tctaatgtta tcaaattcat aaaagataac attcattggc caaatggatt agccattgac 1380
tatgggacaa attatatata ttgggttgat gctaaagaag caaaaattga atgtgtgaaa 1440
ctagatggaa ctgacagaag aaaagtacca tccagtgctg tgaaacatcc cttttctatt 1500
gatatttttg aagatagaat ctactggagt gactgggaca gtgaccaaat tgtatcctgc 1560
aataaatttt ctggtaaaga ttgccataca ataataaagg aaagagatca caagatttat 1620
ggtatccatg tataccatcc tagcatgata aaagaagtta ttaatccatg ctttgatgtt 1680
caatgctctg atatatgctt aatagctcca aaaaatgcag ataatgagtt ggaatattct 1740
tgtgcttgcc catatcacaa aaaacttagc agagatcatc atacatgtat tgaggataaa 1800
ccacttcaaa ctataattgt gggttctgga cagcagatct tcaaaatcca acacaaaaaa 1860
tttggaggat ttgtttcaga ccattttaaa ctaacaatat taaggaaaat aggtgctcta 1920
gcttatgatc caaattcaga taaaatcatt gttagtgatt tggttagacg aaaaattttt 1980
tcagtcgact ggaaaactct agagtcagag ccattaattg aacacaacat tggtagagtt 2040
gttggtatgg atgttgacta ttatgaaaat aatctatatt ggatcgatga tgagaaaaaa 2100
actattgaag ttatgaacct acaaaacaaa caccgattaa cactaatacg agatctaaat 2160
gaaggtctta atgatatagc attagtgtta gaacatgggt ttatgtttgt cgctctttca 2220
acatttgaag gagcacacat tgatagaata tcaatggatg ggcgacatac ttcaaggatt 2280
catgttattg aagacaaatt atttggccca ttgtctctca gctatgatcc taaacttgag 2340
cgtctgttct ggtctgacca aatgggaggt gaaatagctt ccacaggtgt tgatgggatt 2400
gatcgacata tttttaaaga aggacattct ggtccagtag atgtggcaat tactgaatcc 2460
gaagtgtttt ggcttggcta tggagccaga aaagtctttt ggtcaaataa gtttgatgga 2520
tcgcttacaa aaagatttct tttagactca cttgatgaaa ctgataatat gaaaatcatt 2580
ggattaaata aaggtttaag aaaggaagca agtgcttgta attccctcgg aaactgcagc 2640
catgtttgtt tgctttcaac acctatgggg atttgtgcat gtcctgatgg aatggagctt 2700
ggatttgata gaaaaacctg ccaaaaattg cttgcatgta aagaaggaca atacaaatgt 2760
accactggag aatgtatttc aaaatccatg cgatgtaatg gtagaccaga ttgtaggctt 2820
ggtgatgatg aagagtattg ttctataatt tgttcaacaa acaagttcgc ttgcatagat 2880
ggttctagtt gtatagaaca atctcaaaaa tgtgattcta aagttgactg caatgatggt 2940
tctgatgaaa aatattgtga atcaaatcga aattgtactg gaaatgaatt ccaatgtacg 3000
tcaggagaat gtgttcctaa agtggcacag tgtgatggca tgagtgattg cagtgatggt 3060
agtgatgaaa gcagttgtaa tactttcact tgcaaaacca attacttccg ttgcaacaca 3120
ggaaattgta taccaagctc atgggaatgt gacagtcaga ttgactgtag cgacggttct 3180
gatgaaggtc ctaaatgttc tgagattgaa ggatgtggtg aggatcagtt tacttgtgat 3240
aatggtcact gcatttcaca tgttttggtg tgtaaccatg aagatgattg tgaagactca 3300
tctgatgaaa aatattgtta ctttccaaaa accaaaaaaa attatgaaaa tgaagaaaat 3360
aagactaagg gcttatcaaa ggattgccag tttttatgtc cttcagattt aaaaatatgt 3420
ttgcctgcat cagggagatg taatggtact tcagaatgcc cagacggaga agatgaactt 3480
cgttgcaaca agtgttcaga ggaagaattc acttgttctt ctagtaagcg atgtttgcca 3540
atgacatggg tttgtgacaa tgtcagtgac tgtgaagacc agtccgatga aatgcattgt 3600
cttaatggaa ccctggctgt acatgatttg tataatcttc agtgtgatgg tttattctgc 3660
aaagataata aaaaatgcat tccattaaat aaactgtgtg attctcatat ggattgtaat 3720
gatgggtctg atgaaaaagg tctttgtggt aagggatgtg aacatgctgc atgtcatcac 3780
aaatgtaaag agacacccat gggacctagg tgtgaatgtt taagtggttt tactctagaa 3840
ggcgatggga gggcatgtaa tgatataaat gaatgtgaaa aactgtcttc ttgctctcaa 3900
tattgccata attctgttgg cagttacagt tgttcatgca tggattataa ctttcaacta 3960
agatctgata agattaggtg caaagcaaaa ggaactgaaa tgaagtatta ctttgccaca 4020
aaaaaagaaa taaaatataa atcacaatcc ttacagcaaa ctgttgtagt atatcaatca 4080
cagcgtgata tggatatgaa aggaatggat tttgatatga atagtgatgt aatattttgg 4140
acttcagaga ttagtggtta tttgtacaaa gttgatatta aatctaaaaa tgtcactcaa 4200
attacaaatc tcattagacc aactaaagta tcatatgact gggttactgg taatatttat 4260
gtccttgaaa acttcaaaat tattagagtt tgtaacttta aagctaaact ttgttcagca 4320
atatacacag ctaagaatgg tataaacata gaaactattg ttcttgatcc aaaatcaaga 4380
atcatgttct ggtctgaaag taaatggtta atgagccaaa caccaaatag tacacttcaa 4440
caggcctcaa tgagtgcaga aaatccaagt gtagtgctaa gttttgattt tagtccagtt 4500
aatgatattg tagttgatca ttttcacaag gttgtttact ggtcaaaccc cattgataat 4560
aaaatagaaa gatgtacata tgatggaaaa gacagaacta tagtagtgcg tagtgagtat 4620
cctccaagag acttggtact atttgaagat tatttgtact gggtaaatga tcaaaaacaa 4680
tctcacttta ttcgcccaga agcagaagga actattacta agtatggcct gtatggatca 4740
gtgtacatga aacatgaatc agtaaggctt ttaccttctt catcctataa tgaaattaaa 4800
gctttccaag tttcacagtc tgcactccag cctgaaggaa taaaccactg cagtaaaatt 4860
acttgtaatt acctgtgtat aagcaatgaa agagaaccag tatgtttctg tgaagatggc 4920
ttaaaagtga aacctggtga tatatgctac aatcatactg ggatacattt caaagcttac 4980
gaaacagaaa atgtagcaga gagtagttca gtattgacat ggtttttctg gattgttggg 5040
ttagttctag catctggact tggttatgga agttattatt ttgtagctaa ctacgttcag 5100
aaaaataaaa tacacccacg gttcagtgta ttttttcctg ttggccgttc tgagactcat 5160
agagaacaag gatctcaggt tcatcaattt gaaaatccta cgtatagtga ggtcattgtg 5220
gtataa 5226
<210> 26
<211> 5718
<212> DNA
<213> 茶翅蝽
<400> 26
gctgaacctc tgtaccttgg atcttcagtt cccatttcat tttaccaagg cttccagtta 60
gcttcgaact atgctgtcgc cacgatatag gttaaaattt tccaacgatt tgaagttttt 120
gtttgcgact atattaatcg ctttggtcac ttctactgaa cagcatgata cagattgtga 180
tgaaaatttc ttcagatgcc gcaatggtag atgtatatct ctcagcttta tttgcgatgg 240
ttacgaagac tgcggccatg gggacatgtc tgatgaagaa aattgccatt tgaccactac 300
cccagcagtg aaaccgtgca ataaagatga atatcagtgc aaagataggc tgtgcattcc 360
ttcgttatgg gtctgcgatg gtgaatcaga ctgttcggat ggcagcgatg agactcatgg 420
atgtagtgag gcgacctgtg acggttttca gtgccacagc aaatcttgta tccccaaaga 480
gtgggagtgc gatggcgtac gtgattgtcc agataactca gacgaacagt tctgtgctaa 540
gaaaccagtg agcactgaag aatgcaatat tcatgaaaat ggtttcatgt gccatgatag 600
tatgaaatgc attgaactgg aagaagtctg caacagtcac cctgattgcg atgatggttc 660
tgatgaaggt ggtatgtgcc tcaatccgaa caaaacagat ttatgctcta atctgaaatg 720
tcccacaaat caaggctgta taatattacc cgagggcccg gtatgtactg aggtgtgcaa 780
gaaaggatac cacttcgcca gtggttcttg ttttgatatc gatgaatgta taacatttgg 840
aaggtgtgat caaatatgta taaacacaaa tggaggctac aattgctcct gcattgaagg 900
ctatagattt gataatgatt tgaaaaaatg caaagctaga ggaagtgaag gtatactatt 960
tttcacttca aatcaagaaa taagaggata ttatttagac tccgaaatct actttaaagt 1020
agttgaaaat ctaccacatg caacaggagt agcatatgat ggtgttaatg tttactggac 1080
aacagtagca gatgaggaag aaacaattgt caaagcagct gaaaatggtg aaaatcagaa 1140
aataattgta acttctggta ttggcagtcc tgaagatcta gcagttgatt gggttacaaa 1200
aaatgtatac ttcacggatt ctaaattaaa gtatgtaggt gtttgttcca atgatggata 1260
ctattgcctt atactccatg aagatcaagt tgagaaacca cgtgctattg ttctgcattc 1320
atcagaggga ctgatgtatt ggtctgactg gggaacagaa cctgccatta tgcgttctgg 1380
aatggatgga tctaatgtta tcaaattcat aaaagataac attcattggc caaatggatt 1440
agccattgac tatgggacaa attatatata ttgggttgat gctaaagaag caaaaattga 1500
atgtgtgaaa ctagatggaa ctgacagaag aaaagtacca tccagtgctg tgaaacatcc 1560
cttttctatt gatatttttg aagatagaat ctactggagt gactgggaca gtgaccaaat 1620
tgtatcctgc aataaatttt ctggtaaaga ttgccataca ataataaagg aaagagatca 1680
caagatttat ggtatccatg tataccatcc tagcatgata aaagaagtta ttaatccatg 1740
ctttgatgtt caatgctctg atatatgctt aatagctcca aaaaatgcag ataatgagtt 1800
ggaatattct tgtgcttgcc catatcacaa aaaacttagc agagatcatc atacatgtat 1860
tgaggataaa ccacttcaaa ctataattgt gggttctgga cagcagatct tcaaaatcca 1920
acacaaaaaa tttggaggat ttgtttcaga ccattttaaa ctaacaatat taaggaaaat 1980
aggtgctcta gcttatgatc caaattcaga taaaatcatt gttagtgatt tggttagacg 2040
aaaaattttt tcagtcgact ggaaaactct agagtcagag ccattaattg aacacaacat 2100
tggtagagtt gttggtatgg atgttgacta ttatgaaaat aatctatatt ggatcgatga 2160
tgagaaaaaa actattgaag ttatgaacct acaaaacaaa caccgattaa cactaatacg 2220
agatctaaat gaaggtctta atgatatagc attagtgtta gaacatgggt ttatgtttgt 2280
cgctctttca acatttgaag gagcacacat tgatagaata tcaatggatg ggcgacatac 2340
ttcaaggatt catgttattg aagacaaatt atttggccca ttgtctctca gctatgatcc 2400
taaacttgag cgtctgttct ggtctgacca aatgggaggt gaaatagctt ccacaggtgt 2460
tgatgggatt gatcgacata tttttaaaga aggacattct ggtccagtag atgtggcaat 2520
tactgaatcc gaagtgtttt ggcttggcta tggagccaga aaagtctttt ggtcaaataa 2580
gtttgatgga tcgcttacaa aaagatttct tttagactca cttgatgaaa ctgataatat 2640
gaaaatcatt ggattaaata aaggtttaag aaaggaagca agtgcttgta attccctcgg 2700
aaactgcagc catgtttgtt tgctttcaac acctatgggg atttgtgcat gtcctgatgg 2760
aatggagctt ggatttgata gaaaaacctg ccaaaaattg cttgcatgta aagaaggaca 2820
atacaaatgt accactggag aatgtatttc aaaatccatg cgatgtaatg gtagaccaga 2880
ttgtaggctt ggtgatgatg aagagtattg ttctataatt tgttcaacaa acaagttcgc 2940
ttgcatagat ggttctagtt gtatagaaca atctcaaaaa tgtgattcta aagttgactg 3000
caatgatggt tctgatgaaa aatattgtga atcaaatcga aattgtactg gaaatgaatt 3060
ccaatgtacg tcaggagaat gtgttcctaa agtggcacag tgtgatggca tgagtgattg 3120
cagtgatggt agtgatgaaa gcagttgtaa tactttcact tgcaaaacca attacttccg 3180
ttgcaacaca ggaaattgta taccaagctc atgggaatgt gacagtcaga ttgactgtag 3240
cgacggttct gatgaaggtc ctaaatgttc tgagattgaa ggatgtggtg aggatcagtt 3300
tacttgtgat aatggtcact gcatttcaca tgttttggtg tgtaaccatg aagatgattg 3360
tgaagactca tctgatgaaa aatattgtta ctttccaaaa accaaaaaaa attatgaaaa 3420
tgaagaaaat aagactaagg gcttatcaaa ggattgccag tttttatgtc cttcagattt 3480
aaaaatatgt ttgcctgcat cagggagatg taatggtact tcagaatgcc cagacggaga 3540
agatgaactt cgttgcaaca agtgttcaga ggaagaattc acttgttctt ctagtaagcg 3600
atgtttgcca atgacatggg tttgtgacaa tgtcagtgac tgtgaagacc agtccgatga 3660
aatgcattgt cttaatggaa ccctggctgt acatgatttg tataatcttc agtgtgatgg 3720
tttattctgc aaagataata aaaaatgcat tccattaaat aaactgtgtg attctcatat 3780
ggattgtaat gatgggtctg atgaaaaagg tctttgtggt aagggatgtg aacatgctgc 3840
atgtcatcac aaatgtaaag agacacccat gggacctagg tgtgaatgtt taagtggttt 3900
tactctagaa ggcgatggga gggcatgtaa tgatataaat gaatgtgaaa aactgtcttc 3960
ttgctctcaa tattgccata attctgttgg cagttacagt tgttcatgca tggattataa 4020
ctttcaacta agatctgata agattaggtg caaagcaaaa ggaactgaaa tgaagtatta 4080
ctttgccaca aaaaaagaaa taaaatataa atcacaatcc ttacagcaaa ctgttgtagt 4140
atatcaatca cagcgtgata tggatatgaa aggaatggat tttgatatga atagtgatgt 4200
aatattttgg acttcagaga ttagtggtta tttgtacaaa gttgatatta aatctaaaaa 4260
tgtcactcaa attacaaatc tcattagacc aactaaagta tcatatgact gggttactgg 4320
taatatttat gtccttgaaa acttcaaaat tattagagtt tgtaacttta aagctaaact 4380
ttgttcagca atatacacag ctaagaatgg tataaacata gaaactattg ttcttgatcc 4440
aaaatcaaga atcatgttct ggtctgaaag taaatggtta atgagccaaa caccaaatag 4500
tacacttcaa caggcctcaa tgagtgcaga aaatccaagt gtagtgctaa gttttgattt 4560
tagtccagtt aatgatattg tagttgatca ttttcacaag gttgtttact ggtcaaaccc 4620
cattgataat aaaatagaaa gatgtacata tgatggaaaa gacagaacta tagtagtgcg 4680
tagtgagtat cctccaagag acttggtact atttgaagat tatttgtact gggtaaatga 4740
tcaaaaacaa tctcacttta ttcgcccaga agcagaagga actattacta agtatggcct 4800
gtatggatca gtgtacatga aacatgaatc agtaaggctt ttaccttctt catcctataa 4860
tgaaattaaa gctttccaag tttcacagtc tgcactccag cctgaaggaa taaaccactg 4920
cagtaaaatt acttgtaatt acctgtgtat aagcaatgaa agagaaccag tatgtttctg 4980
tgaagatggc ttaaaagtga aacctggtga tatatgctac aatcatactg ggatacattt 5040
caaagcttac gaaacagaaa atgtagcaga gagtagttca gtattgacat ggtttttctg 5100
gattgttggg ttagttctag catctggact tggttatgga agttattatt ttgtagctaa 5160
ctacgttcag aaaaataaaa tacacccacg gttcagtgta ttttttcctg ttggccgttc 5220
tgagactcat agagaacaag gatctcaggt tcatcaattt gaaaatccta cgtatagtga 5280
ggtcattgtg gtataataca atataaatat gatgtttatt taagaaaaaa caaatatatg 5340
catatatata tgtatacact tatatataca catatatatg tatatattta tagatacaca 5400
cattaaatta agttgtttat ctttttctat aggagaactc tgaatttaaa tctttgttaa 5460
acaatgaaat atctgtgaat caagtgtaga aataattgat ttacattgta aacctccgtg 5520
taacaagttt cttattaaca ttatattatt accatatcat ataaatatga atttcctagt 5580
aactgattgt tcttattact atttttgact gattaaagta atgtactctc agtttaaatc 5640
agcaaagagc tatttaagct ctataaaact gtattattat caaatattta atatttgaac 5700
tgtaatttat tgttttaa 5718
<210> 27
<211> 5307
<212> DNA
<213> 豌豆长管蚜
<400> 27
atggacaatg gcgctgacga agacgattgt gatattagca aaaagccttg cctggcgcca 60
gaatactttg aatgtgataa caagagaaaa tgcattccta aggaatttgt gtgtgataag 120
tcgaatgaat gcatcgacaa gtctgacgaa agtcattgta aagagacgtc atcatcttta 180
acgcagtcat gccgggaagg aaaattcaaa tgctccgatg gcacttgtat tccaatggct 240
tggcattgtg acgcggagat tgattgccac gacggtagcg atgaagacgg agaaaaatgt 300
agatacacga cattgtgtac ctctggttat atgtgcaaaa acttcaattg cgtttcaaag 360
acgtggagat gtgacggaaa agatgactgt ggagatggtt cagacgaaac ccattgcgat 420
atccaactgg ttgagccaga aaaatgttta attgaaaatc gtaaatttct atgtcacgat 480
cataaaaaat gtatcgatgt cagaaatgtc tgtgattaca acaatgactg tcttgatgca 540
tcggacgaag gtggtctttg caataataaa actgatttca aaagtgaatg taattcgatg 600
aattgttctg ccgcatttga atgtttaaga aagccacatg gcattatgtg tgtgtgccca 660
aaaggaatgc attatttaaa taataaatgt tctgatatca acgaatgtga acaatatggt 720
atttgtgacc aagtgtgtct taatttggaa ggttcatata cgtgctcctg tgatccacat 780
tatgaactag tagataatca taagtgtaaa ataaaaggcc taaatccgga attattatat 840
tcatcactac gtcaaataaa agtttttaac ttggaattac ttcatagttt tactttgatt 900
gatgatttgc aacatgtcac tggattagct gttgataaat taactttata ctggacactt 960
tatttggatg ggaactcagc tattgttaga gcaaacaagt ctgagcctaa accagaaatt 1020
attgttgact cgggtcttgg atcgcctgag aatctagtta tagatattat tacacataat 1080
ttgtacttca ccgatgcaaa aatgaaacat attggagtgt gcaataatga tggttcagta 1140
tgtacagttc ttcataataa aaacatagac aaaccaagag cagtagctgt ttcaccacta 1200
gatggtctca tgtactggac agattggggt aaaaatccaa tgattggtcg ttctggtatg 1260
gacgggtcaa gaccacaacc gtttgttaca caaaatatac actggccaaa tggtgttcat 1320
gttgattatg tcgggaaaag aatttactgg gtggatgcta agatgcaatt tattgaatcc 1380
attaatttag atgcaactga tcgaagagta attgtaacag aatatgtaga tcatccatat 1440
tcggttactg tgttcgagga taaattgtac tggagtgatt ggtcaagtaa agaaataaaa 1500
gtatgcaaca aatttacggg taaagatagc aagacattga tacgtgaaaa taagaacaga 1560
gtgtatggca tgcaaattaa ccatccctcg ctcttcgagt ctcaaatgcc aaatccttgt 1620
gaacaaggaa aatgcagtga tatatgctta ttagcaccga aaacagaaat caattctaaa 1680
ggatactcgt gcgcttgccc cgatgataaa aaactatcgg aagatggaat cttttgtatc 1740
accattgcca tacctcctac gcttattgtg ggtacaccaa caagtattat agaaattgag 1800
caggaacatt taggccgcca aaaagcaaaa aaaatatcgt tgaaaaatga tatatctagc 1860
atatccgcat tgacttataa ttcattatct ggtgacatca tcatctatga ctcaaggtct 1920
aagaaattat tcactttcga ttttgttaag atgaaattat cagatttaga tgtaccagaa 1980
attagttcca tttattctct tgaattcgac aatcatggaa acaatttata ctggtgtgat 2040
aggactagaa aaacattaga tgtgttaagt ctctctacaa aaacacacac aacgatattc 2100
aaagaaattg aaggtcatat cccatttgcc gttactttag ttccagataa aagctttatg 2160
tttgtagcta tgaaagaagg ttcacacata catatcgatc gaattgacat ggatggaagt 2220
attcagtctc tagtacatat ggtggagtat ggattgacag gagatgaaat tgctttacat 2280
tttgatatga ttacaagact gttgtatttt actgactata aaaatggcat tattgatagt 2340
gtcagcgaag atggaacgga tagaagaatt ataaaaaaag caggatttgt aagagatatt 2400
gtttcaattg gttatgacat tgtttgggta tctgaaggtt cgaaaatttt aaactggatt 2460
gataacgttg atgaagaccg agcatctaga gctcttgata tagataattg gaaaactgat 2520
tcaaatttat atttaaccgt agcaaatgga attcacaaaa atatacaaca cccgtgtcaa 2580
caaaataatg gtggttgtag tcatatatgc cttttatctg agaaaggaaa ggtttgtggt 2640
tgtccacctg gattagtttt atcaaacaac agcctgaatt gtacatcact aggagagtgt 2700
aaatctgatg aatatcgttg ttacactggt gaatgtattt catctcattc ccgatgtgat 2760
tttataaaag attgtcctcg tggtgacgat gaagacgttg ttaaatgttt gcattacaca 2820
ccaaactcat gtccacaatc tcaattttta tgtcatgata aaacaaagtg cttggataaa 2880
aaacatatgt gtgatagtat taaagactgt agtgatggtt cagatgaaat ggattgttta 2940
cacaagcaca cttgtgattc aactacagaa tatcagtgta catctggaga gtgtattcag 3000
cgcatatttt tatgtgatgg taatcccgac tgtattaatg gacaagacga gttaaattgt 3060
ctgaaccaaa gttgtaataa tattacagaa tttagatgta attctggaaa ctgtatacct 3120
gcaacatggg aatgcgatgg tgaggttgat tgttttgatg gttcggatga acactattca 3180
tgtgctacca aaaaatgtaa agatgatcag ttttcttgca ccaatggtcg atgtatatca 3240
cataaattca cttgtaacgg taaagatgat tgtggtgata gttctgatga aaatggttgt 3300
tcgtcaaatc atgcatacat gacaaaaaga gtttcaaaag tatgtaatga aaaaacagaa 3360
tttgaatgtg aaaacacaat gggacattgt ataccgatca aggctaggtg caacggcaca 3420
tccgagtgca aacatttaga ggacgaattg aattgtggat gtcaaaaatc taattttttc 3480
gaatgtcaga ataagcgttg tgtgttaaaa gattggttat gtgataaaca tgatgattgt 3540
ggcgatgggt cagatgaaag tcaaaaggcg tgtgatatgc tttctgaaca tttatcgaat 3600
tcagtcgttt catcaaaaga ctgtgatgga tatttatgta agaaccaaga atgtatacct 3660
ctagatcaag cttgtaataa gaaaatcaat tgtaaagatg gttcagacga aggagatctt 3720
tgtggtttat cttgtataca catggattgt agtcatactt gtcaagaaac tccaaaaggt 3780
gggaaatgta catgttatca tggctataaa cttgcacaag atggtataac ttgcagagat 3840
attaatgaat gtgaagacga caatttatgt actcaatatt gtacaaattc agatggttca 3900
tatagctgta gttgtttaaa ttctgattac attttgcgtg cagataaaag ttcctgtaaa 3960
gcaattggac ccacaatgga tttagtttat tcgagtgttg atgaaataag aagtacttca 4020
ggagatatta aggaatcaaa attaattttt tcgatgcctg gaatgacaat atcgagcctt 4080
gatatagata ttagaagaaa tcttatttat tggacatcaa aacaagctgg tgttcttatt 4140
tgtatggata tgcaacaaca gcataaattt tacatgacag atttgttaca cccaacttta 4200
gttcgtattg attggttaac agaaaacgtt tactttgtac aaaattttaa agatattgtt 4260
gtatgttatt taaatgctaa acgttgtgcc actatataca gtgcaaacat ccatacaaca 4320
attatggctt ttgaaattga tcctcgttcc ggagtgatgt tctggtcgga aaccatatgg 4380
gcagtatttt caacaccaac aactattatt aggaaatctg attgcagtgg atacaatgta 4440
aaaactataa tccatcaagg gttacagtat gttactgatt tagctataga tccaatcaaa 4500
catatggtct attgggtcga tcaagttaat tcaacaatcg agaggtctaa ttacgacgga 4560
actaaaataa ctgtgttggt caattcgata gaacatttgc cagagaaaat atcattgtac 4620
gaagacaaac tgttttggac caaccatgaa accgggttgt ctatatttaa atgtaaagtt 4680
ctcggttctg aaaatgtcca ctgtgagtct gttcctgttc aagtgtttgc taccatcgag 4740
acattcacga taagtcaaca ggccaaacaa agaaatggat caaatgtttg cattgatgtc 4800
gattgcgatt ttatttgtac ttctggatca aaaggaccga tgtgtgtttg tggagatgga 4860
tctcaagtcg agccaggcaa tatttgtgat gatgctggtc atagaaaatt gcctgttttt 4920
aaagacttag attacaaaaa aaatatgatt tcaatctata tttctatatt tttaattgta 4980
atgttgttat tagcgatgtt atattatttt ctattctatt ccaaaaataa atctttgaat 5040
caactagtat ctagattatt tcatcggcca ttaacaactg tcatccaagt aattgaccag 5100
aaagaagatg aaataaattc aagatataat gtttatgaga atcctatgaa cgaagatggg 5160
ttaataaaag accatgcttg tggaagtgaa atcgtcttga actatgatag tgcacgttac 5220
gttaatgcta actgctcaga acctccactt aactcgtttt cagagtacga cgagaacgag 5280
aaaaaaaaac ttattatggt tttataa 5307
<210> 28
<211> 5772
<212> DNA
<213> 豌豆长管蚜
<400> 28
attgaatgtt ttttcagaaa aagaatgtac taaatacgaa ttcaactgcg ggaacggtac 60
ttgtatcaat aagacaagac tttgtgacgg cattgttgac tgtatggaca atggcgctga 120
cgaagacgat tgtgatatta gcaaaaagcc ttgcctggcg ccagaatact ttgaatgtga 180
taacaagaga aaatgcattc ctaaggaatt tgtgtgtgat aagtcgaatg aatgcatcga 240
caagtctgac gaaagtcatt gtaaagagac gtcatcatct ttaacgcagt catgccggga 300
aggaaaattc aaatgctccg atggcacttg tattccaatg gcttggcatt gtgacgcgga 360
gattgattgc cacgacggta gcgatgaaga cggagaaaaa tgtagataca cgacattgtg 420
tacctctggt tatatgtgca aaaacttcaa ttgcgtttca aagacgtgga gatgtgacgg 480
aaaagatgac tgtggagatg gttcagacga aacccattgc gatatccaac tggttgagcc 540
agaaaaatgt ttaattgaaa atcgtaaatt tctatgtcac gatcataaaa aatgtatcga 600
tgtcagaaat gtctgtgatt acaacaatga ctgtcttgat gcatcggacg aaggtggtct 660
ttgcaataat aaaactgatt tcaaaagtga atgtaattcg atgaattgtt ctgccgcatt 720
tgaatgttta agaaagccac atggcattat gtgtgtgtgc ccaaaaggaa tgcattattt 780
aaataataaa tgttctgata tcaacgaatg tgaacaatat ggtatttgtg accaagtgtg 840
tcttaatttg gaaggttcat atacgtgctc ctgtgatcca cattatgaac tagtagataa 900
tcataagtgt aaaataaaag gcctaaatcc ggaattatta tattcatcac tacgtcaaat 960
aaaagttttt aacttggaat tacttcatag ttttactttg attgatgatt tgcaacatgt 1020
cactggatta gctgttgata aattaacttt atactggaca ctttatttgg atgggaactc 1080
agctattgtt agagcaaaca agtctgagcc taaaccagaa attattgttg actcgggtct 1140
tggatcgcct gagaatctag ttatagatat tattacacat aatttgtact tcaccgatgc 1200
aaaaatgaaa catattggag tgtgcaataa tgatggttca gtatgtacag ttcttcataa 1260
taaaaacata gacaaaccaa gagcagtagc tgtttcacca ctagatggtc tcatgtactg 1320
gacagattgg ggtaaaaatc caatgattgg tcgttctggt atggacgggt caagaccaca 1380
accgtttgtt acacaaaata tacactggcc aaatggtgtt catgttgatt atgtcgggaa 1440
aagaatttac tgggtggatg ctaagatgca atttattgaa tccattaatt tagatgcaac 1500
tgatcgaaga gtaattgtaa cagaatatgt agatcatcca tattcggtta ctgtgttcga 1560
ggataaattg tactggagtg attggtcaag taaagaaata aaagtatgca acaaatttac 1620
gggtaaagat agcaagacat tgatacgtga aaataagaac agagtgtatg gcatgcaaat 1680
taaccatccc tcgctcttcg agtctcaaat gccaaatcct tgtgaacaag gaaaatgcag 1740
tgatatatgc ttattagcac cgaaaacaga aatcaattct aaaggatact cgtgcgcttg 1800
ccccgatgat aaaaaactat cggaagatgg aatcttttgt atcaccattg ccatacctcc 1860
tacgcttatt gtgggtacac caacaagtat tatagaaatt gagcaggaac atttaggccg 1920
ccaaaaagca aaaaaaatat cgttgaaaaa tgatatatct agcatatccg cattgactta 1980
taattcatta tctggtgaca tcatcatcta tgactcaagg tctaagaaat tattcacttt 2040
cgattttgtt aagatgaaat tatcagattt agatgtacca gaaattagtt ccatttattc 2100
tcttgaattc gacaatcatg gaaacaattt atactggtgt gataggacta gaaaaacatt 2160
agatgtgtta agtctctcta caaaaacaca cacaacgata ttcaaagaaa ttgaaggtca 2220
tatcccattt gccgttactt tagttccaga taaaagcttt atgtttgtag ctatgaaaga 2280
aggttcacac atacatatcg atcgaattga catggatgga agtattcagt ctctagtaca 2340
tatggtggag tatggattga caggagatga aattgcttta cattttgata tgattacaag 2400
actgttgtat tttactgact ataaaaatgg cattattgat agtgtcagcg aagatggaac 2460
ggatagaaga attataaaaa aagcaggatt tgtaagagat attgtttcaa ttggttatga 2520
cattgtttgg gtatctgaag gttcgaaaat tttaaactgg attgataacg ttgatgaaga 2580
ccgagcatct agagctcttg atatagataa ttggaaaact gattcaaatt tatatttaac 2640
cgtagcaaat ggaattcaca aaaatataca acacccgtgt caacaaaata atggtggttg 2700
tagtcatata tgccttttat ctgagaaagg aaaggtttgt ggttgtccac ctggattagt 2760
tttatcaaac aacagcctga attgtacatc actaggagag tgtaaatctg atgaatatcg 2820
ttgttacact ggtgaatgta tttcatctca ttcccgatgt gattttataa aagattgtcc 2880
tcgtggtgac gatgaagacg ttgttaaatg tttgcattac acaccaaact catgtccaca 2940
atctcaattt ttatgtcatg ataaaacaaa gtgcttggat aaaaaacata tgtgtgatag 3000
tattaaagac tgtagtgatg gttcagatga aatggattgt ttacacaagc acacttgtga 3060
ttcaactaca gaatatcagt gtacatctgg agagtgtatt cagcgcatat ttttatgtga 3120
tggtaatccc gactgtatta atggacaaga cgagttaaat tgtctgaacc aaagttgtaa 3180
taatattaca gaatttagat gtaattctgg aaactgtata cctgcaacat gggaatgcga 3240
tggtgaggtt gattgttttg atggttcgga tgaacactat tcatgtgcta ccaaaaaatg 3300
taaagatgat cagttttctt gcaccaatgg tcgatgtata tcacataaat tcacttgtaa 3360
cggtaaagat gattgtggtg atagttctga tgaaaatggt tgttcgtcaa atcatgcata 3420
catgacaaaa agagtttcaa aagtatgtaa tgaaaaaaca gaatttgaat gtgaaaacac 3480
aatgggacat tgtataccga tcaaggctag gtgcaacggc acatccgagt gcaaacattt 3540
agaggacgaa ttgaattgtg gatgtcaaaa atctaatttt ttcgaatgtc agaataagcg 3600
ttgtgtgtta aaagattggt tatgtgataa acatgatgat tgtggcgatg ggtcagatga 3660
aagtcaaaag gcgtgtgata tgctttctga acatttatcg aattcagtcg tttcatcaaa 3720
agactgtgat ggatatttat gtaagaacca agaatgtata cctctagatc aagcttgtaa 3780
taagaaaatc aattgtaaag atggttcaga cgaaggagat ctttgtggtt tatcttgtat 3840
acacatggat tgtagtcata cttgtcaaga aactccaaaa ggtgggaaat gtacatgtta 3900
tcatggctat aaacttgcac aagatggtat aacttgcaga gatattaatg aatgtgaaga 3960
cgacaattta tgtactcaat attgtacaaa ttcagatggt tcatatagct gtagttgttt 4020
aaattctgat tacattttgc gtgcagataa aagttcctgt aaagcaattg gacccacaat 4080
ggatttagtt tattcgagtg ttgatgaaat aagaagtact tcaggagata ttaaggaatc 4140
aaaattaatt ttttcgatgc ctggaatgac aatatcgagc cttgatatag atattagaag 4200
aaatcttatt tattggacat caaaacaagc tggtgttctt atttgtatgg atatgcaaca 4260
acagcataaa ttttacatga cagatttgtt acacccaact ttagttcgta ttgattggtt 4320
aacagaaaac gtttactttg tacaaaattt taaagatatt gttgtatgtt atttaaatgc 4380
taaacgttgt gccactatat acagtgcaaa catccataca acaattatgg cttttgaaat 4440
tgatcctcgt tccggagtga tgttctggtc ggaaaccata tgggcagtat tttcaacacc 4500
aacaactatt attaggaaat ctgattgcag tggatacaat gtaaaaacta taatccatca 4560
agggttacag tatgttactg atttagctat agatccaatc aaacatatgg tctattgggt 4620
cgatcaagtt aattcaacaa tcgagaggtc taattacgac ggaactaaaa taactgtgtt 4680
ggtcaattcg atagaacatt tgccagagaa aatatcattg tacgaagaca aactgttttg 4740
gaccaaccat gaaaccgggt tgtctatatt taaatgtaaa gttctcggtt ctgaaaatgt 4800
ccactgtgag tctgttcctg ttcaagtgtt tgctaccatc gagacattca cgataagtca 4860
acaggccaaa caaagaaatg gatcaaatgt ttgcattgat gtcgattgcg attttatttg 4920
tacttctgga tcaaaaggac cgatgtgtgt ttgtggagat ggatctcaag tcgagccagg 4980
caatatttgt gatgatgctg gtcatagaaa attgcctgtt tttaaagact tagattacaa 5040
aaaaaatatg atttcaatct atatttctat atttttaatt gtaatgttgt tattagcgat 5100
gttatattat tttctattct attccaaaaa taaatctttg aatcaactag tatctagatt 5160
atttcatcgg ccattaacaa ctgtcatcca agtaattgac cagaaagaag atgaaataaa 5220
ttcaagatat aatgtttatg agaatcctat gaacgaagat gggttaataa aagaccatgc 5280
ttgtggaagt gaaatcgtct tgaactatga tagtgcacgt tacgttaatg ctaactgctc 5340
agaacctcca cttaactcgt tttcagagta cgacgagaac gagaaaaaaa aacttattat 5400
ggttttataa gcatcataaa attataaact ttgttaagga tgaatattta tttgttaatt 5460
tttattttta taaatacaat tttattttat ttttataata tcaatatgca cattgcacat 5520
agatataaat tattactgaa atttataatt ctgtatacct aaatgattat tttttttagt 5580
ttgttaactt ttacaatagt tccttatttt taatttgttt aattcaaagt tacccggtat 5640
atatatatta ttataataat aaataaaagt tacattaaat tagtgtgatt gtgtcaatat 5700
atcaaaagct aacacttaat ataattcaat gtatattcaa aagatctgcg tgtgtaatga 5760
aattaaatat at 5772
<210> 29
<211> 5430
<212> DNA
<213> 烟粉虱
<400> 29
atgattcaga gggaatggag ctccatatcc aagggctcat ggtgcacagc attattagta 60
gttatcgcag tcttttgcac atttgtacaa tcatcttcat catatgaatg tgtcggacca 120
tcccatttcg aatgtacaaa tcacaggtgt atatccatgg atttaaggtg cgatggagat 180
gacgattgta atgatggatc cgatgaacac ggttgtaatg tggataaatc aaaaaatgaa 240
acctgtgcta gtacccagtt tgattgcggt cagggccaat gtataccacg gtcctgggtt 300
tgtgatggga atgcagattg tgaggacggt aaagatgaag gagctgtagg ctgcgctgaa 360
agccattgcg cagcgtctga gtgggagtgc cctcataacc atcgctgtat cccgaatgat 420
tatatctgtg atggggatga tgattgtggt gacaattcag atgaagacga ctgtacaggc 480
aagaataatt ttacagagtg cacttcagcg tttggaaaat ttctctgcaa aaataggaat 540
cagtgcatcg atgattctct tctgtgcaac gggcaccctg actgtaaaga tgggtcagat 600
gagggtggtc actgtgcttc gaaagctcaa gtagctactg actgtgcaaa gctaaactgt 660
acccactcgt gtgttgaaag cccagatgga ccggtttgcg tctgtggatc aggatatcac 720
cttgaaggaa acgtctgtga agatattaat gagtgcttgg aatggggaac atgtgaccaa 780
atgtgtgaaa acacggtcgg aggttacatc tgtgaatgtg aacctggata caaactggaa 840
agtaacggac gcacatgcaa agcagaagag ggtgaaggac ttctgatcta ttcaagcctc 900
aaaaagatta agtctctata tctgacatct cgcatatcaa tgacagttgc ctctgaggtt 960
ccatatgcga caggtgtgtc ttttgatggt aaacatgtct attggacgac cgtgcttgat 1020
ggagttgaat ctattgttcg ggcaagtgag gatggatctc atgaaaccac cattgttgat 1080
tcaggtgttg gctcccctga agacctagct gtcgactggg tgactggtaa catatacttc 1140
actgatggcg agtaccaaca gatcggcatt tgcacctaca acgaagagct ggttgaaacg 1200
aaatgcgctg tcctccacaa caaagacctg aacaagcccc gcgcaattgt tttaaaccca 1260
gctgatgcgg tcatgtactg gtctgattgg ggctttaaac cactcatcgc ccgttctgga 1320
atggatggca cagacttcta tgagtttgta acgacagagc tccattggcc caatggtctc 1380
acaattgatc acggaaatcg gagagtatat tgggtcgatg cgagactcgg aactgttgaa 1440
actgttgatt ttcaaggccg tgatcggcgg aaaatattaa ctgatctcaa tgatcatcct 1500
ttcgcaatcg ctgtttttga agataaaatc tattggagtg gatggacaaa ccaagaaata 1560
gtagaatgta ataagttcac cgggaaaaac cgagtacaag ttgtcaaaag tcggaaagac 1620
aaaatttacg gtgtgcacat ttttcaccca actttgcaaa atcattcgct accaaatccg 1680
tgcgccggga agtgcagtga catctgtgct ctatctccat cagcttcaag cggaggcaaa 1740
ggctactctt gcttgtgccc tgacaataaa atcttatccc cctctggaga atggtgccaa 1800
gaacagccca aagaatctgt cattgttagc attggaaact ttgttttcca gctgaaagtg 1860
actcttggga agcagtacat tcatccgctg cctgttaata acctccagtc tgtcagtgca 1920
atcgtctaca actcctttga tggctctcta ttaatagctg acccagatgc caaaatgatt 1980
tactcgtatc aactgaacac ggacaccatg gagactctaa ttgatctcaa agtgggctat 2040
gtctcagcat tggcttacga tcccattggc cggaatctct attggtgcga caaagaggcc 2100
ggcactgtag aagtgttcag tttcttctct cacagaagga agttgttgct ccgggaattt 2160
gacgatgaaa aacctttcgc catgactctc ataccagaag aaggcctgat gttcgtcatt 2220
gccaaagccc atgaccacct tcacattgat cggatcaaca tggatggctc actaagcaca 2280
ctgactcaca tgaccagtct caagttgcaa ggaccggatg tagctttgca ctatgacagt 2340
gattctcgca gggtttattg ggctgatcac tcagctggcc tcattgagag caccgatacg 2400
aatggaaacg acaggcaagt ataccgtgac gtatcatcac ctttagctct gaccgatgtg 2460
gatagagacc tttattggac atcagatggt cgcccacacc tgtactactc tgagaaaagc 2520
aatgcaagca tgcccgtcag gaaaatcaac atggaacgat ttttgcgatc accaaaggac 2580
cactatcgga tgtttgttac tgcaatcatc cctgataaaa ctactagaga tcatccttgc 2640
caaactaaca atggcaagtg tagccatttt tgcctcctca ccagtcgtaa tccaaagcat 2700
gtatgcagtt gtcctgatgg gatgaagctg gcggataatg gtcaggactg tgaagagatt 2760
gctgcatgtg gagcacatga ataccattgc acgacaggtg aatgcattcc aatgtcgaag 2820
aaatgcgacc gaaacaagga ttgcccctat ggcgaagatg agaccttctg cccagcacag 2880
tgtgagactg atcagtttgc gtgctttgat ggacaaaaat gtatcgatgc caaggataga 2940
tgcaacatgc attttgattg tcacgaccac tctgatgagg ctaactgcca aaatgtcacc 3000
tgcgatcaat catacaactt tttgtgccga atcggtgagt gcgtaagtca cgctgtgctg 3060
tgcaataatg aatggaattg caaggacggt agtgatgaag agaactgtac gacttccacg 3120
tgcccttcca gcgagtttcg gtgtcattcc ggaacatgca taccgaagaa ctgggtctgt 3180
gatctggatg ccgattgccc cgaccaatca gatgaaaata actgcagttt ctcaagaaaa 3240
gaaaagtgca ccgaattctt gtgtcagagt ggcatgtgtg tagcacagga acttgtctgt 3300
aacggtcaaa ccgaatgtga cgatggaagt gacgaattca attgcgatga gccggttcca 3360
aaaacggcaa acaaagagga tggttttata gataactgtg atgaagagaa ggagttcatg 3420
tgcgagcctg gcaaatgcat taatcttata ttcaagtgta atggtgttaa agattgcgag 3480
aacggagctg atgagctaaa ctgcataggc tgtgaacaat tcacttgcaa taatgggaaa 3540
tgcatcactt atgatcttgt atgcaatgat gatgatgact gtggtgactc atccgatgaa 3600
agacctctca actcgtgccc agacagcaaa gaaaacccag ctattgtgcc tgctcatatt 3660
cccaatgtgt gccatggatt tgtttgcaag aacggtgaat gccttgatga tttcagtcta 3720
gtctgcaaca aaaagcaaga ttgtaaggac ggctcagatg aaggtggacg atgcggttca 3780
agctgtgatg tgacagcaaa ttgcagtcag atttgtcgtg ataagccaaa tggacatgag 3840
tgtgcgtgtg tccctggctt taaaattgct gaggatggcc gcgattgcga ggatattgat 3900
gagtgcaccg aattggagcc gtgtagccag atgtgtttca acacctatgg aagttacacc 3960
tgtgcctgtc ttggtccaga ttatattaaa aagtcagatg ggtcctgcaa agccactggc 4020
ccaaaattgc agtacgtctt tgctaccggt taccagattc gcactatatc atacctaatg 4080
accgatgtca aagtggcata ctatagtgca gatcttgaag tttcaggatt tgatgtgaac 4140
atgagaacag agcatgtcta ctggtcatct gaaaacaagg gtgtcatcac aaaaatgtca 4200
ttaacgcaca gacacgagcc aaaacatttc atcactggtt tacgccgtcc atcagagctg 4260
gcagtcgatt ggataacaca caacctctac tttgtccaag caaggaacat cattaatgtc 4320
tgtaacttcc accttgaacg ctgcgcacaa attttgaccg ctgaaagtgg cttggagatc 4380
aacagtcttg ccgttgatcc tgtcagaggt gtccttttct ggagtgaaac aagccgcatc 4440
gtctggaata tgcctaagag ctccattaga cgcgcagaca tgaatggaaa aaatattgag 4500
accattgttt cagctaatgt gagctacgca ttggatcttg ctttagatcc aatcctcaat 4560
catgtctatt gggtcgacaa aacattgaag gtcattgaaa gagctaacta tgatggaact 4620
cgccgacgtg tcatcttgac atcaaagttc catccaaaat ctgttgcgct ctttgatggt 4680
tctatctact ggtcggttga gtcgagtgga tccccgatta caaagtgtgc tcttcaagga 4740
ctttccacgg aatcgtactc gtgcaatcaa atcccaatca aagttgtaga tccaataact 4800
cacttcactc tcatgcaacc agctctgcaa agaaacatat caaacgcttg taggaacatg 4860
gagtgcagtc acatgtgcgt tctcagctcg acattaccat catgcatttg tcgcaatgga 4920
aagatagttc ctccaaaaac agcctgtacg gatagcaatt atatgccaga aacccacttt 4980
ttggaaacaa ctggaactgt tgatggtcaa agtcctggat attcttggtc atcaatctgt 5040
gcaacaatca tcttagtcgc tttcatcgga acaactttct atgccttatt ctactactat 5100
aactccaagt acaacatgcg acgtctattt ccatcaattc attttaaaaa tccagccttc 5160
aacctacagt caaaattcca ggctaatggg atgaccggtt tagccagtgg caaccatatg 5220
gctcacttgt catcgaagga ccatcatttc gaaaaccctc tgcaagagag tcgtgaaggt 5280
gaagtgagaa tcgttactcc aaatgaaatc acgattagcc gagccgagac cagttggaca 5340
tcagcccatc tagaagactc aagctctata gaaacagaat acgcagacct tgttgtcgag 5400
acaaacccca aagctaattt gatatcgtga 5430
<210> 30
<211> 5774
<212> DNA
<213> 烟粉虱
<400> 30
atgattcaga gggaatggag ctccatatcc aagggctcat ggtgcacagc attattagta 60
gttatcgcag tcttttgcac atttgtacaa tcatcttcat catatgaatg tgtcggacca 120
tcccatttcg aatgtacaaa tcacaggtgt atatccatgg atttaaggtg cgatggagat 180
gacgattgta atgatggatc cgatgaacac ggttgtaatg tggataaatc aaaaaatgaa 240
acctgtgcta gtacccagtt tgattgcggt cagggccaat gtataccacg gtcctgggtt 300
tgtgatggga atgcagattg tgaggacggt aaagatgaag gagctgtagg ctgcgctgaa 360
agccattgcg cagcgtctga gtgggagtgc cctcataacc atcgctgtat cccgaatgat 420
tatatctgtg atggggatga tgattgtggt gacaattcag atgaagacga ctgtacaggc 480
aagaataatt ttacagagtg cacttcagcg tttggaaaat ttctctgcaa aaataggaat 540
cagtgcatcg atgattctct tctgtgcaac gggcaccctg actgtaaaga tgggtcagat 600
gagggtggtc actgtgcttc gaaagctcaa gtagctactg actgtgcaaa gctaaactgt 660
acccactcgt gtgttgaaag cccagatgga ccggtttgcg tctgtggatc aggatatcac 720
cttgaaggaa acgtctgtga agatattaat gagtgcttgg aatggggaac atgtgaccaa 780
atgtgtgaaa acacggtcgg aggttacatc tgtgaatgtg aacctggata caaactggaa 840
agtaacggac gcacatgcaa agcagaagag ggtgaaggac ttctgatcta ttcaagcctc 900
aaaaagatta agtctctata tctgacatct cgcatatcaa tgacagttgc ctctgaggtt 960
ccatatgcga caggtgtgtc ttttgatggt aaacatgtct attggacgac cgtgcttgat 1020
ggagttgaat ctattgttcg ggcaagtgag gatggatctc atgaaaccac cattgttgat 1080
tcaggtgttg gctcccctga agacctagct gtcgactggg tgactggtaa catatacttc 1140
actgatggcg agtaccaaca gatcggcatt tgcacctaca acgaagagct ggttgaaacg 1200
aaatgcgctg tcctccacaa caaagacctg aacaagcccc gcgcaattgt tttaaaccca 1260
gctgatgcgg tcatgtactg gtctgattgg ggctttaaac cactcatcgc ccgttctgga 1320
atggatggca cagacttcta tgagtttgta acgacagagc tccattggcc caatggtctc 1380
acaattgatc acggaaatcg gagagtatat tgggtcgatg cgagactcgg aactgttgaa 1440
actgttgatt ttcaaggccg tgatcggcgg aaaatattaa ctgatctcaa tgatcatcct 1500
ttcgcaatcg ctgtttttga agataaaatc tattggagtg gatggacaaa ccaagaaata 1560
gtagaatgta ataagttcac cgggaaaaac cgagtacaag ttgtcaaaag tcggaaagac 1620
aaaatttacg gtgtgcacat ttttcaccca actttgcaaa atcattcgct accaaatccg 1680
tgcgccggga agtgcagtga catctgtgct ctatctccat cagcttcaag cggaggcaaa 1740
ggctactctt gcttgtgccc tgacaataaa atcttatccc cctctggaga atggtgccaa 1800
gaacagccca aagaatctgt cattgttagc attggaaact ttgttttcca gctgaaagtg 1860
actcttggga agcagtacat tcatccgctg cctgttaata acctccagtc tgtcagtgca 1920
atcgtctaca actcctttga tggctctcta ttaatagctg acccagatgc caaaatgatt 1980
tactcgtatc aactgaacac ggacaccatg gagactctaa ttgatctcaa agtgggctat 2040
gtctcagcat tggcttacga tcccattggc cggaatctct attggtgcga caaagaggcc 2100
ggcactgtag aagtgttcag tttcttctct cacagaagga agttgttgct ccgggaattt 2160
gacgatgaaa aacctttcgc catgactctc ataccagaag aaggcctgat gttcgtcatt 2220
gccaaagccc atgaccacct tcacattgat cggatcaaca tggatggctc actaagcaca 2280
ctgactcaca tgaccagtct caagttgcaa ggaccggatg tagctttgca ctatgacagt 2340
gattctcgca gggtttattg ggctgatcac tcagctggcc tcattgagag caccgatacg 2400
aatggaaacg acaggcaagt ataccgtgac gtatcatcac ctttagctct gaccgatgtg 2460
gatagagacc tttattggac atcagatggt cgcccacacc tgtactactc tgagaaaagc 2520
aatgcaagca tgcccgtcag gaaaatcaac atggaacgat ttttgcgatc accaaaggac 2580
cactatcgga tgtttgttac tgcaatcatc cctgataaaa ctactagaga tcatccttgc 2640
caaactaaca atggcaagtg tagccatttt tgcctcctca ccagtcgtaa tccaaagcat 2700
gtatgcagtt gtcctgatgg gatgaagctg gcggataatg gtcaggactg tgaagagatt 2760
gctgcatgtg gagcacatga ataccattgc acgacaggtg aatgcattcc aatgtcgaag 2820
aaatgcgacc gaaacaagga ttgcccctat ggcgaagatg agaccttctg cccagcacag 2880
tgtgagactg atcagtttgc gtgctttgat ggacaaaaat gtatcgatgc caaggataga 2940
tgcaacatgc attttgattg tcacgaccac tctgatgagg ctaactgcca aaatgtcacc 3000
tgcgatcaat catacaactt tttgtgccga atcggtgagt gcgtaagtca cgctgtgctg 3060
tgcaataatg aatggaattg caaggacggt agtgatgaag agaactgtac gacttccacg 3120
tgcccttcca gcgagtttcg gtgtcattcc ggaacatgca taccgaagaa ctgggtctgt 3180
gatctggatg ccgattgccc cgaccaatca gatgaaaata actgcagttt ctcaagaaaa 3240
gaaaagtgca ccgaattctt gtgtcagagt ggcatgtgtg tagcacagga acttgtctgt 3300
aacggtcaaa ccgaatgtga cgatggaagt gacgaattca attgcgatga gccggttcca 3360
aaaacggcaa acaaagagga tggttttata gataactgtg atgaagagaa ggagttcatg 3420
tgcgagcctg gcaaatgcat taatcttata ttcaagtgta atggtgttaa agattgcgag 3480
aacggagctg atgagctaaa ctgcataggc tgtgaacaat tcacttgcaa taatgggaaa 3540
tgcatcactt atgatcttgt atgcaatgat gatgatgact gtggtgactc atccgatgaa 3600
agacctctca actcgtgccc agacagcaaa gaaaacccag ctattgtgcc tgctcatatt 3660
cccaatgtgt gccatggatt tgtttgcaag aacggtgaat gccttgatga tttcagtcta 3720
gtctgcaaca aaaagcaaga ttgtaaggac ggctcagatg aaggtggacg atgcggttca 3780
agctgtgatg tgacagcaaa ttgcagtcag atttgtcgtg ataagccaaa tggacatgag 3840
tgtgcgtgtg tccctggctt taaaattgct gaggatggcc gcgattgcga ggatattgat 3900
gagtgcaccg aattggagcc gtgtagccag atgtgtttca acacctatgg aagttacacc 3960
tgtgcctgtc ttggtccaga ttatattaaa aagtcagatg ggtcctgcaa agccactggc 4020
ccaaaattgc agtacgtctt tgctaccggt taccagattc gcactatatc atacctaatg 4080
accgatgtca aagtggcata ctatagtgca gatcttgaag tttcaggatt tgatgtgaac 4140
atgagaacag agcatgtcta ctggtcatct gaaaacaagg gtgtcatcac aaaaatgtca 4200
ttaacgcaca gacacgagcc aaaacatttc atcactggtt tacgccgtcc atcagagctg 4260
gcagtcgatt ggataacaca caacctctac tttgtccaag caaggaacat cattaatgtc 4320
tgtaacttcc accttgaacg ctgcgcacaa attttgaccg ctgaaagtgg cttggagatc 4380
aacagtcttg ccgttgatcc tgtcagaggt gtccttttct ggagtgaaac aagccgcatc 4440
gtctggaata tgcctaagag ctccattaga cgcgcagaca tgaatggaaa aaatattgag 4500
accattgttt cagctaatgt gagctacgca ttggatcttg ctttagatcc aatcctcaat 4560
catgtctatt gggtcgacaa aacattgaag gtcattgaaa gagctaacta tgatggaact 4620
cgccgacgtg tcatcttgac atcaaagttc catccaaaat ctgttgcgct ctttgatggt 4680
tctatctact ggtcggttga gtcgagtgga tccccgatta caaagtgtgc tcttcaagga 4740
ctttccacgg aatcgtactc gtgcaatcaa atcccaatca aagttgtaga tccaataact 4800
cacttcactc tcatgcaacc agctctgcaa agaaacatat caaacgcttg taggaacatg 4860
gagtgcagtc acatgtgcgt tctcagctcg acattaccat catgcatttg tcgcaatgga 4920
aagatagttc ctccaaaaac agcctgtacg gatagcaatt atatgccaga aacccacttt 4980
ttggaaacaa ctggaactgt tgatggtcaa agtcctggat attcttggtc atcaatctgt 5040
gcaacaatca tcttagtcgc tttcatcgga acaactttct atgccttatt ctactactat 5100
aactccaagt acaacatgcg acgtctattt ccatcaattc attttaaaaa tccagccttc 5160
aacctacagt caaaattcca ggctaatggg atgaccggtt tagccagtgg caaccatatg 5220
gctcacttgt catcgaagga ccatcatttc gaaaaccctc tgcaagagag tcgtgaaggt 5280
gaagtgagaa tcgttactcc aaatgaaatc acgattagcc gagccgagac cagttggaca 5340
tcagcccatc tagaagactc aagctctata gaaacagaat acgcagacct tgttgtcgag 5400
acaaacccca aagctaattt gatatcgtga tactctttaa tcatttgaaa aatgcagtcg 5460
tgtaaatatg ttgtacagtt tgttttttgt tgaatctttt aatttatcta tgcatttgtt 5520
gtttgtataa atttttatta ccatattttg gtgtcttcgg aactgaagtc agtcaaattt 5580
atcgagtcaa atttcatatc gatcagatca gtttatggtg tttttatatt tgtttaaaga 5640
agattgatcc atttttatca aaagcatctg tcaattatct tccttagaaa aatgatacaa 5700
tctaaatttt ttcaaattta taatacaagg aaaaaaaaaa aacctatagt gaaatcacta 5760
gtggaggatc cgcg 5774
<210> 31
<211> 5370
<212> DNA
<213> 斜纹夜蛾
<400> 31
atgaggaaat atagtaacaa aatgaagtat caaggcttgc tattgatagt tagtgtggcg 60
tggtgttcag ctcagctcag cgatgacatg cagatgtttg acccagaata catggcggaa 120
gacaagttcc cctgcatggg tggaggatgt atttcagtgt cacagtactg cgatggaaaa 180
ttggactgtg atgatggcag cgacgagaat ttttgcatcg aacacaagcc gttccaggag 240
ttttgtaacg agatccacca gtacatgtgt cgggactcca cgaagtgtgt tcctttatcc 300
tggctgtgca acaatgagcc ggactgcgat gatggcagcg atgagttcaa ctgcaccgca 360
ctgccggctg taaacgtcaa ttcgacatgc aaaggcttcc aatgtggaga cggcaaatgc 420
atctccttcc tgtgggtgtg tgacggtgtc tacgactgtg aagataaaag cgatgagtat 480
gctgaggaac tctgcaggca cgtatcccgt ccccacgcga tcgtcgacgg ttcatactgt 540
caggaattgc acacaatgga cgaccgtaac tacaagtgtc tggacgcctc cttctgcctg 600
cccagcagta tgatgtgcga cggactccag gattgccgcg atggcagcga tgagggacca 660
ttttgcaaaa actggaatac aatgtgcgac aacttcaaat gcatgggtaa cgacacgata 720
tgttccccgg agaggtttgg agcctcctgc ctctgcctgc cctcccactt catgcggcaa 780
tacgattaca tcaccaaaca gtgccaggat gtcaacgagt gcctcatgga gcacccaccc 840
tgctcccata aatgcattaa cgccgatggc cattatattt gtgaatgcga tcctggatac 900
aaacgggatg tgtatggata tctttgttat gctactggtc ccgaagcgat gctgttcttc 960
aatactcgga acgatatcag atacctgaag attaagacta aagaaatggt cacagtggct 1020
accgacatca aagagggtca tggcgtttca ttcgacggga cttacattta ttgggtggag 1080
acagcacagg gacatcagtc catcttcaaa gcacaactcg gagacgtcaa ggatacaaag 1140
gaggtactgg taggtctcgg tctggaggac cctggcgaca tagcagtgga ctacctgggc 1200
gggaacatat acttcagtga tgcggagcga ggaatcatct ccgcttgcag ggtcgacggc 1260
tccatctgca ccacgatcaa gactcatgct aacaacccac gatttgtcac tcttgatcct 1320
aaaaatggta agatgtactg ggcagactgg cacgagcgtc cagtgataat gtcagctcgg 1380
atggacggca gccgacacga tgagctggtg gacaacttgg agaactttgc caccggcctg 1440
gccgtggacg cgccaaacgg gagactgtac ttcgttgaca aaactatcaa agtcgtcatg 1500
attgcggaga aacatgttta tgctttattt gaagagccct tccaccatcc gtactccatc 1560
tcagtgttcg agaacactgt gttctggagt gactggacct ccaacagtat acagacgact 1620
gacaaagtgc acgggaccgc gcagaagagg aatgttctac tcaaacttga tacacctgta 1680
ctaggcatgc acatgtacca tccagtgctg atgaacacga catcgaaccc gtgcagcaac 1740
aacaactgct cgcatctctg cttcgtcacc tccaacgcca cccacgtctg cgcctgcccc 1800
gatggcatgg agattgagaa taatcagtgc catcacgtag gcaactaccg cgccaagtac 1860
ctggtggtgg gaagtgggca gctgttcaca aagatccagt acaacgcgct cggcaacccg 1920
gagtgtcacg ccacgcactt cgaaatcgga cgcgtgcagg ccatggcgta cgatcggtat 1980
agagattcac tattcatata cgacggccag cgacggacca tcaactacat aaacatgagt 2040
gacttcactc tcggtatcac tcacgtgttg atctacaacg gactggagaa cgtcgtcgac 2100
atggattatg attacgtgac agataacctg tacgtgctgg atgcaagtcg tcgtgtcgtt 2160
gaagctgtgt ctctgaggac tcagaagcgc gccatagtac atcgattcga cattcaagaa 2220
ctgcctgtta gcttctgtat tctttcggac tacgggagaa tgttggtggc cgtggtggag 2280
agtgagatgc acaacaccat tcatatcgac agcatcgggc tcgacgggac ccaacggaga 2340
cacgtcatca tgaacaattt gaaaggtcct catattcgat tgcggtacgt gccagatttt 2400
attgggcgcg actggaccca acggagacac gtcatcatga acaatttgaa aggtcctcat 2460
attcgattgc ggtacgtgcc agaaactgag caggtgttca tatccgacga gagcaacggc 2520
atcatcgatt tcatacatcc tgaaggcacc gggcgggaga actatcggga gctgacctcg 2580
acggtgacca gcctcgccat agcagataac tacgtcttct ggacagaccg aaagaccccc 2640
agactcttct ggtctgacat acacgaagcc tcgccgaaga taaggaggat tgatctggca 2700
cttttcccaa acactacgca acttctgatc caagcaacga actcgctccc tgatccaaaa 2760
gaccctcttc tgaaccatcc ctgtctaaag aatccttgtt ctgacgtctg tgtccaactt 2820
ccccatgata ctccagaaga tcacccgaaa ctagcaaact tcgagatgaa gtacaagtgt 2880
ttgtgtcctc ctgggttgtt ggtgaatggt aaccagtgcg ccaaacctgc gacatgtggt 2940
tcggatgaga tactgtgcca taggagtaat atttgtgtga agcaagatgc caggtgtgat 3000
ggaaaggccg actgtccgaa gaatgaagac gaggaaggtt gtatagtgga tccggcaaac 3060
atatgttcat cagacgaaat attctgtcgc ggcttgtgcg taaacaggga caaagccacg 3120
acgtgttcta ccggagaaaa aactaataaa gcactacctt cgaacaactg cagcagcaca 3180
gaattccagt gcacggacac ctcaatctgc atctctcggt cgcaggtctg tgaccaacac 3240
atcgactgtc ctaatggatc ggatgagcag ctcttagagt gcgacatcta ctcttgtcat 3300
gaaactgagt tcatgtgtgc gtccggctca tgtatagtga aaacgtggac atgtgatgga 3360
gaccgtgact gtaatgatgg atctgatgaa atcaactgcg tgaacatgac ttgcggcctg 3420
ggcttctacc agtgcagaga cagggagtgc gtggagttat cgaagaggtg tgacggacat 3480
cgggattgct cggattactc cgacgaagag gactgtgatg agcctcaaat tgtcgagacg 3540
atagaagaag ccccgaaatg tgctgcgtgg gaatatactt gtgaaaaaaa tactagcatt 3600
tgtttgcctg aaaccgctcg atgcaacatg aagacggact gcccgggtgg cacggatgag 3660
cacggttgcg accaccgctg tgccccgaag ggaatgtttg cgtgcggcca acaggtcact 3720
tgtatcacaa tgaacaaggt ctgcaacgga cgactggatt gtgatgacgg atccgatgag 3780
acgcctgatg cttgttctca ggtgaacagg acctctcacc tgttcccagt agctcggaca 3840
ttcactgaat gtactgaggg ttataagtgt aacaacggac agtgcattga gtggtcacag 3900
ttgtgtgaca agaagaggga ctgcatcgat gggacagatg aaaatggact ttgcgatact 3960
gcatgcgcaa acagcacatg tacgttcatg tgtcagccga ccccattcgg acgtcgctgt 4020
ctgtgcccct ttggcttcca aatttccaag gatcagttct cgtgcgagga catcgacgag 4080
tgcactgaag atgtctgctc tcaagggtgt atcaacgtgc cagggtcctt cctgtgctgg 4140
tgccatcatg gttatgccat caggaggaca gaccgtcgct attgcaaggc gatccgcggc 4200
aacatgtcga tcctgtacgt gtcgggtaac accgtgcggt ctatctcagc agacgggtac 4260
ggtgctatcg agtataccga tactaatgcc tcggccatca ctgatatgga ttataatgtt 4320
cggcagaaga agctatacgt gacctcggaa gcgggtggca agttgttaga agtgaatgag 4380
acgcagaatg tgatcgctgt tacgaacgtt gggaaaccat cgagggtggc agtggactgg 4440
gtgaccggca acgtgtactt cgtggacacg acgccatttg accagcgcat acgagtctgt 4500
catgtcaaga ggaagcgctg cgcttccctg ctgaaacttc cttctgatgc cacggtgaca 4560
gcgttgatag tagagcccag ttccagccgc atgttctact gcgtcactcg gaagctggag 4620
tcggtgatct ggacagcgaa cctggctggc agacacgtga cggatctggc tactgtacgg 4680
aactgcaccg gtctggccgc agactccttc aagaagaagc tgtacgtcgc agagacgggc 4740
ccagcgcata ttattaggat ggattatgag ggggagaatt tcaacaaaat cctctcagat 4800
cacccacgtc tgcaagcacc gcatggacta gaaatattcg aagactacat ctactacttg 4860
gaagccaact cgttccgcct cagccgctgc cagctgtacg gggccaaaca ttgtgaaact 4920
tatgtgtacc gtgtgtttga cgccaacacg ttcgtcattc gccacgagag catccaacgc 4980
gatgacatag tcaacgaatg tgaagatgtc gtttgtgaca acatttgtgc cgtggacgaa 5040
gatgggccca aatgtttgtg tgacgatgga gccttggcca agagtgggaa gtgtcctgtg 5100
gttgataaga aattggtacc tttattcaac ggctggtcgt acgagcagct ggcatcggct 5160
cgcagcatcc ccttcaccgt caccttggga gtactgaccc tgatcgccat ctacctctgc 5220
gtcttcgtat actaccattg tatatacata cccaggaaga gaatgttagc atctgcttac 5280
actgaagttc ggttccagaa cacacaaaat acgtcctatc ctgaatcaga cccggtagtg 5340
gagatgcatc cctcaaggta tctaccttag 5370
<210> 32
<211> 5370
<212> DNA
<213> 斜纹夜蛾
<400> 32
atgaggaaat atagtaacaa aatgaagtat caaggcttgc tattgatagt tagtgtggcg 60
tggtgttcag ctcagctcag cgatgacatg cagatgtttg acccagaata catggcggaa 120
gacaagttcc cctgcatggg tggaggatgt atttcagtgt cacagtactg cgatggaaaa 180
ttggactgtg atgatggcag cgacgagaat ttttgcatcg aacacaagcc gttccaggag 240
ttttgtaacg agatccacca gtacatgtgt cgggactcca cgaagtgtgt tcctttatcc 300
tggctgtgca acaatgagcc ggactgcgat gatggcagcg atgagttcaa ctgcaccgca 360
ctgccggctg taaacgtcaa ttcgacatgc aaaggcttcc aatgtggaga cggcaaatgc 420
atctccttcc tgtgggtgtg tgacggtgtc tacgactgtg aagataaaag cgatgagtat 480
gctgaggaac tctgcaggca cgtatcccgt ccccacgcga tcgtcgacgg ttcatactgt 540
caggaattgc acacaatgga cgaccgtaac tacaagtgtc tggacgcctc cttctgcctg 600
cccagcagta tgatgtgcga cggactccag gattgccgcg atggcagcga tgagggacca 660
ttttgcaaaa actggaatac aatgtgcgac aacttcaaat gcatgggtaa cgacacgata 720
tgttccccgg agaggtttgg agcctcctgc ctctgcctgc cctcccactt catgcggcaa 780
tacgattaca tcaccaaaca gtgccaggat gtcaacgagt gcctcatgga gcacccaccc 840
tgctcccata aatgcattaa cgccgatggc cattatattt gtgaatgcga tcctggatac 900
aaacgggatg tgtatggata tctttgttat gctactggtc ccgaagcgat gctgttcttc 960
aatactcgga acgatatcag atacctgaag attaagacta aagaaatggt cacagtggct 1020
accgacatca aagagggtca tggcgtttca ttcgacggga cttacattta ttgggtggag 1080
acagcacagg gacatcagtc catcttcaaa gcacaactcg gagacgtcaa ggatacaaag 1140
gaggtactgg taggtctcgg tctggaggac cctggcgaca tagcagtgga ctacctgggc 1200
gggaacatat acttcagtga tgcggagcga ggaatcatct ccgcttgcag ggtcgacggc 1260
tccatctgca ccacgatcaa gactcatgct aacaacccac gatttgtcac tcttgatcct 1320
aaaaatggta agatgtactg ggcagactgg cacgagcgtc cagtgataat gtcagctcgg 1380
atggacggca gccgacacga tgagctggtg gacaacttgg agaactttgc caccggcctg 1440
gccgtggacg cgccaaacgg gagactgtac ttcgttgaca aaactatcaa agtcgtcatg 1500
attgcggaga aacatgttta tgctttattt gaagagccct tccaccatcc gtactccatc 1560
tcagtgttcg agaacactgt gttctggagt gactggacct ccaacagtat acagacgact 1620
gacaaagtgc acgggaccgc gcagaagagg aatgttctac tcaaacttga tacacctgta 1680
ctaggcatgc acatgtacca tccagtgctg atgaacacga catcgaaccc gtgcagcaac 1740
aacaactgct cgcatctctg cttcgtcacc tccaacgcca cccacgtctg cgcctgcccc 1800
gatggcatgg agattgagaa taatcagtgc catcacgtag gcaactaccg cgccaagtac 1860
ctggtggtgg gaagtgggca gctgttcaca aagatccagt acaacgcgct cggcaacccg 1920
gagtgtcacg ccacgcactt cgaaatcgga cgcgtgcagg ccatggcgta cgatcggtat 1980
agagattcac tattcatata cgacggccag cgacggacca tcaactacat aaacatgagt 2040
gacttcactc tcggtatcac tcacgtgttg atctacaacg gactggagaa cgtcgtcgac 2100
atggattatg attacgtgac agataacctg tacgtgctgg atgcaagtcg tcgtgtcgtt 2160
gaagctgtgt ctctgaggac tcagaagcgc gccatagtac atcgattcga cattcaagaa 2220
ctgcctgtta gcttctgtat tctttcggac tacgggagaa tgttggtggc cgtggtggag 2280
agtgagatgc acaacaccat tcatatcgac agcatcgggc tcgacgggac ccaacggaga 2340
cacgtcatca tgaacaattt gaaaggtcct catattcgat tgcggtacgt gccagatttt 2400
attgggcgcg actggaccca acggagacac gtcatcatga acaatttgaa aggtcctcat 2460
attcgattgc ggtacgtgcc agaaactgag caggtgttca tatccgacga gagcaacggc 2520
atcatcgatt tcatacatcc tgaaggcacc gggcgggaga actatcggga gctgacctcg 2580
acggtgacca gcctcgccat agcagataac tacgtcttct ggacagaccg aaagaccccc 2640
agactcttct ggtctgacat acacgaagcc tcgccgaaga taaggaggat tgatctggca 2700
cttttcccaa acactacgca acttctgatc caagcaacga actcgctccc tgatccaaaa 2760
gaccctcttc tgaaccatcc ctgtctaaag aatccttgtt ctgacgtctg tgtccaactt 2820
ccccatgata ctccagaaga tcacccgaaa ctagcaaact tcgagatgaa gtacaagtgt 2880
ttgtgtcctc ctgggttgtt ggtgaatggt aaccagtgcg ccaaacctgc gacatgtggt 2940
tcggatgaga tactgtgcca taggagtaat atttgtgtga agcaagatgc caggtgtgat 3000
ggaaaggccg actgtccgaa gaatgaagac gaggaaggtt gtatagtgga tccggcaaac 3060
atatgttcat cagacgaaat attctgtcgc ggcttgtgcg taaacaggga caaagccacg 3120
acgtgttcta ccggagaaaa aactaataaa gcactacctt cgaacaactg cagcagcaca 3180
gaattccagt gcacggacac ctcaatctgc atctctcggt cgcaggtctg tgaccaacac 3240
atcgactgtc ctaatggatc ggatgagcag ctcttagagt gcgacatcta ctcttgtcat 3300
gaaactgagt tcatgtgtgc gtccggctca tgtatagtga aaacgtggac atgtgatgga 3360
gaccgtgact gtaatgatgg atctgatgaa atcaactgcg tgaacatgac ttgcggcctg 3420
ggcttctacc agtgcagaga cagggagtgc gtggagttat cgaagaggtg tgacggacat 3480
cgggattgct cggattactc cgacgaagag gactgtgatg agcctcaaat tgtcgagacg 3540
atagaagaag ccccgaaatg tgctgcgtgg gaatatactt gtgaaaaaaa tactagcatt 3600
tgtttgcctg aaaccgctcg atgcaacatg aagacggact gcccgggtgg cacggatgag 3660
cacggttgcg accaccgctg tgccccgaag ggaatgtttg cgtgcggcca acaggtcact 3720
tgtatcacaa tgaacaaggt ctgcaacgga cgactggatt gtgatgacgg atccgatgag 3780
acgcctgatg cttgttctca ggtgaacagg acctctcacc tgttcccagt agctcggaca 3840
ttcactgaat gtactgaggg ttataagtgt aacaacggac agtgcattga gtggtcacag 3900
ttgtgtgaca agaagaggga ctgcatcgat gggacagatg aaaatggact ttgcgatact 3960
gcatgcgcaa acagcacatg tacgttcatg tgtcagccga ccccattcgg acgtcgctgt 4020
ctgtgcccct ttggcttcca aatttccaag gatcagttct cgtgcgagga catcgacgag 4080
tgcactgaag atgtctgctc tcaagggtgt atcaacgtgc cagggtcctt cctgtgctgg 4140
tgccatcatg gttatgccat caggaggaca gaccgtcgct attgcaaggc gatccgcggc 4200
aacatgtcga tcctgtacgt gtcgggtaac accgtgcggt ctatctcagc agacgggtac 4260
ggtgctatcg agtataccga tactaatgcc tcggccatca ctgatatgga ttataatgtt 4320
cggcagaaga agctatacgt gacctcggaa gcgggtggca agttgttaga agtgaatgag 4380
acgcagaatg tgatcgctgt tacgaacgtt gggaaaccat cgagggtggc agtggactgg 4440
gtgaccggca acgtgtactt cgtggacacg acgccatttg accagcgcat acgagtctgt 4500
catgtcaaga ggaagcgctg cgcttccctg ctgaaacttc cttctgatgc cacggtgaca 4560
gcgttgatag tagagcccag ttccagccgc atgttctact gcgtcactcg gaagctggag 4620
tcggtgatct ggacagcgaa cctggctggc agacacgtga cggatctggc tactgtacgg 4680
aactgcaccg gtctggccgc agactccttc aagaagaagc tgtacgtcgc agagacgggc 4740
ccagcgcata ttattaggat ggattatgag ggggagaatt tcaacaaaat cctctcagat 4800
cacccacgtc tgcaagcacc gcatggacta gaaatattcg aagactacat ctactacttg 4860
gaagccaact cgttccgcct cagccgctgc cagctgtacg gggccaaaca ttgtgaaact 4920
tatgtgtacc gtgtgtttga cgccaacacg ttcgtcattc gccacgagag catccaacgc 4980
gatgacatag tcaacgaatg tgaagatgtc gtttgtgaca acatttgtgc cgtggacgaa 5040
gatgggccca aatgtttgtg tgacgatgga gccttggcca agagtgggaa gtgtcctgtg 5100
gttgataaga aattggtacc tttattcaac ggctggtcgt acgagcagct ggcatcggct 5160
cgcagcatcc ccttcaccgt caccttggga gtactgaccc tgatcgccat ctacctctgc 5220
gtcttcgtat actaccattg tatatacata cccaggaaga gaatgttagc atctgcttac 5280
actgaagttc ggttccagaa cacacaaaat acgtcctatc ctgaatcaga cccggtagtg 5340
gagatgcatc cctcaaggta tctaccttag 5370
<210> 33
<211> 5341
<212> DNA
<213> 萝卜菜跳甲
<400> 33
tcagcagttt tcgcacccga aggaaattgc acctccgacc agttccaatg ctccgaccag 60
cgatgcataa actttaaagc cagatgcgac tcaaaactgg attgcccgga cggatcggac 120
gaattagaat gcgatctgca tcgctgcgat gagccggagt tcttccgctg taaaaataac 180
agatgcatca gtcacgcttt cgtctgcgat aaacagaacg attgcgagga cttctccgac 240
gaggcgaact gcgacagctt ccagtctttg gtgtcttcgg cttgcgaaga gggccactgg 300
aagtgcgccg acaaattgtg catacccaac gattgggtgt gcgacggcac gccggaatgc 360
atcgacggat ccgacgagac catcggctgc agcacccaga tcgcctgcga cgggttccgc 420
tgcaagaaca agcactgcgt gcccaaagag tgggtctgcg acaaccacaa cgattgcatc 480
gataacagcg acgaggagga ctgcgagaac cacatcccca tcgaccagtg cacgctggac 540
aagcgcaagt tcctctgcca caacaacgtg acttgcattg acaccgagtc ggtttgcgat 600
ggagtcaata attgtccaga caattcggac gaatccctgc aatgtaacgc gtcagtgaag 660
gcttgcaaag gcgatcacca gtgctcgcac gtttgcatcc cgctgcccga aggccccaag 720
tgcgtctgtc ccgcggggta ccacacgctc gacgagaaaa actgcgagga tatcaacgaa 780
tgcgaaacat acggtatatg cgatcaaatg tgccggaaca cgccgggttc gtacgaatgc 840
tactgcgaca aggcgtaccg tttggcggag gacaagaaaa cctgcaaggc ggagggcgag 900
gtgggcatca tggtgttcag ctcgaaggat caaatcagag cggtggcgct ggattcgacc 960
agctacatca tcgtcgccaa gaacttgaag aaagtggtgg gcgtcgattt cgacggtcgc 1020
cgggtctact ggacggagat atacggggga tacgagaaca tcgccaggag cgccgaggac 1080
gggtctcaga aggaggtgtt agtaacggct ggcgtagatc taccagagga tttgtccatc 1140
gattggttga cgggtaacat ttatttcacc gatggcgaga agcagcacat cggagtttgc 1200
tcgccggacg ggacccattg cgctgtttta atcaataagg acatcaggaa acccagaggg 1260
atcgttctta acgtggtgga cggcgacatg tactggacgg attgggaaga tccggcgcga 1320
atcggttact cgctaatgga cggatcaaat gataagcctt tcgtggaaaa agacatacat 1380
tggcccaacg gtttagcatt ggatcatcct aatagtagat tgtactggac ggatgctagg 1440
aagatgacat tggagagcat acgtttagat ggaactgatc gaaggataat attagaagac 1500
atcgtgaagc atccgtacgc catagcagta ttcgaggata aattgtactg gtcggattgg 1560
gcgacgcgaa ccatccaaca gtgcgacaaa ttcgacggga agaaccacaa aacgttgatc 1620
gaagagaaag agctcatcta cgggatcagc atattccatt cggcgcagca gaagcgcagc 1680
aaaaacccct gcgagatggc gctgtgcagc gacttgtgct tactgaaagg tacatcgtac 1740
agctgcgctt gtccacagaa caaggtctta caatccgacg ggcttttttg taaagaatta 1800
tcgcctacag aaactcttat cgccgctcat cgggatatgc tagtacatat agagcatccg 1860
attcttggta ggcatgttgt tacagctcta cccggcgcca ctgcagacat agaaagcatg 1920
acattcgaca gctataagaa tgtactgtac gtcagcgact tcaagaccag gaggatcagt 1980
actttgcaca tgaagaccgg tagtagcaaa gttatggaca tttccgattt gggtagaatc 2040
agcgctatgg atttcgattc taaaagcaac aatctttaca tctgcgattc ggtacgcaaa 2100
gtggtggaag taatcagtct caacacgatg gcaaggcgga ttttaatcca cgacacgttc 2160
ggagaaatcc cccaaagtat cgcgttagtc cccgacgaag gggtgatgtt tgtaagcttc 2220
ggaggcgagg gacccagcca catcgatcgc ttctccatgg acggtacgga tcgaactcac 2280
tctatagaca ccaaactaac ggggccagtg tcactggcgt acgatcccga tttacacagg 2340
gtgttcttcg ccgacgccac caacggctta atcgaatcca ccaacgtgga cggcgacgat 2400
cgcatccact tcagaaccgt cgattctcac cccatcagct tgctggtgct caaagacgat 2460
gtgttctggg tgaacgtgca ctcgaagcag ctctactgga cctcgaagaa agtcccatcg 2520
aattacgaca agaaaattac gctggcgttc cccgacgatc cggacaaggt gcacttggtg 2580
tcagtaacgt cgcgccatgt agagagcaac ttatgtcgca tcaacaacaa cggttgcagc 2640
catctgtgct tgcagtcgca gaagtccatc gtgtgccagt gcccgatcgg ctgggagctg 2700
aaggaggaca accgcacctg cgccaagaga gtgggttgca tcggcggcga gtttttgtgc 2760
caccactcca acacttgcat tttgaaaagc ctcagatgca acggccacaa ggactgcttg 2820
tttggagaag acgagagcga ttgcgcagct gcagcgaaat gcggaggaag cgagttccaa 2880
tgcgccaacg gcgattgcat agctgcggat ctggcctgca accatcggta tgattgcaag 2940
gataaatctg acgagcacgg ttgcgatgat gttaagaacg gcactcgatg cccgccagat 3000
cacttcacct gctcgaacgg ggattgcatc agccagcatt tccactgcga tggcgtctcc 3060
gattgcacag acaactcaga cgagattcac tgccaggtga acgattgtaa tgcaacgcaa 3120
tttaggtgcg attcgggctc ttgcattccc aaggaatggg agtgcgatca cgattacgat 3180
tgcatggata gttctgatga gcattgcaat gcgctgtgcc cggagagcca cttcaaatgc 3240
gacaacggcc tgtgcgtgga caagaagtta atgtgcgacg gtttcgacaa ctgcggcgac 3300
cactccgacg agaagatgca catgtgccac cagagggccg cgcacaactg caccgcctac 3360
gagacggcct gctcgtccaa cgcctccatt tgcattcctt tggaagcgaa atgcgacggg 3420
aaatccgact gtcccaagca cgaagacgag atcggctgcg ccatgtgcgc cgacgaggat 3480
ttcgagtgcc gcaacaagca tttgaaggac tgcattccga ggatgtggct gtgcgacggc 3540
atgaacgatt gcggggacaa cagcgacgag gacttggtga tgtgcagcaa aaagaacaga 3600
accatgttcg ctccggccca agtaccctgc accgatggct tcaggtgcga taacggcaac 3660
tgcatcaacg catccttgct ctgcaacggg aaacacgact gctatgatgg ctctgacgaa 3720
ggaggcttgt gcaccgggtc ctgcgaaggc tctaagaatc cttgtaatca catctgcatc 3780
aaaacaccga aaggtcctaa atgccagtgc aggtcgggct acaagttgat gggcgatggt 3840
aaaacgtgcg ttgatgagaa cgaatgcgag gtctatcctc ccatatgcag ccaaatttgc 3900
cgcaacagag aaggcggtta cacttgcgat tgcttccaca acttttattt aagaaaggac 3960
atgaaatcct gcaaagcggc gggttccgac atgttgatct acttcaacgt gaacggcaat 4020
caaatctacg aaatcgcgcc gaagaacaat tcgatgagcg tcgtccagga agagtcgatg 4080
ctgaagatca cgagtctgga tactctggtg gactctaagg agctcttctt cagcgtgcag 4140
gacaccggcg ccatctacaa gctggatccg gctacaggaa ccatgcacta catcaagaac 4200
ttgggcgagc cgaagctgat agccgtggat tggtcgacgg ggaatgttta ttatcacaac 4260
gcccaatcgg acgcgaaatc gatcggggtg tgcaattacg aggagaagtg cgccaaattg 4320
atcgacatcg acgcgcatcg acaggtgtcc gcattggctg tggattcttt caacaaagtt 4380
ttgttctatg tggtcagatc gtggacgata ttcgcatcgc ccagctacgt tatatacaaa 4440
accgatttgg acggtagcaa cgtggtggaa ctggtcaaaa caaatatagg caacgtggaa 4500
cacatcacct acgatttgaa caaacgacag ctgtacttcc acgacgagaa cacccagcaa 4560
atcaacgtga tcggctacga agggggccgc accaaatccc tcttctacaa cgtgacttta 4620
atcgaagggc tcagactgtt cgaagataat ttgtactact tgcgccagga cgggttcctc 4680
atcagatgcc agttgttcga agaggccgcc tgtcactacg gtttcaactt gcacagtttg 4740
gctagggacg agttcgtaat ctcgcacaga tcccttcagc ccttcgttgg gcacgtctgc 4800
gatgggcatg cttgccccta catgtgcgtg gctggacaag agggctattt gtgcgtttgc 4860
catgacggct cgaccagtgg cgaatgcggc acaccgcaag acgagaacgg cgagaacaaa 4920
ttcaaagtaa cttccatcga gggaaaatcc agatcttcgg cgtttttgtc cggtttcgcg 4980
gtgtttgggg tactagtagc atgcgtcatg attatacttg gggcttatta catgataaag 5040
aggagaagcg gtgataacag taccgatttg agcatcgtgc gatttcaaaa tccgctttac 5100
ggaagacccg tggaggacga gaaacctatt ttggagcccg ggaagcacga gtacgtgaat 5160
tgtttgtacc aacacaaagc ggatggtggc gaaaatagca atactgaacc caaaacgttc 5220
aacgtctgtg atgataattt gtgttaattt attgtttttt tatgttttac gttaattagg 5280
tgcattattt gattgttaat tctatttata acacattatt atatgtatat acaaattgtt 5340
a 5341
<210> 34
<211> 5341
<212> DNA
<213> 萝卜菜跳甲
<400> 34
tcagcagttt tcgcacccga aggaaattgc acctccgacc agttccaatg ctccgaccag 60
cgatgcataa actttaaagc cagatgcgac tcaaaactgg attgcccgga cggatcggac 120
gaattagaat gcgatctgca tcgctgcgat gagccggagt tcttccgctg taaaaataac 180
agatgcatca gtcacgcttt cgtctgcgat aaacagaacg attgcgagga cttctccgac 240
gaggcgaact gcgacagctt ccagtctttg gtgtcttcgg cttgcgaaga gggccactgg 300
aagtgcgccg acaaattgtg catacccaac gattgggtgt gcgacggcac gccggaatgc 360
atcgacggat ccgacgagac catcggctgc agcacccaga tcgcctgcga cgggttccgc 420
tgcaagaaca agcactgcgt gcccaaagag tgggtctgcg acaaccacaa cgattgcatc 480
gataacagcg acgaggagga ctgcgagaac cacatcccca tcgaccagtg cacgctggac 540
aagcgcaagt tcctctgcca caacaacgtg acttgcattg acaccgagtc ggtttgcgat 600
ggagtcaata attgtccaga caattcggac gaatccctgc aatgtaacgc gtcagtgaag 660
gcttgcaaag gcgatcacca gtgctcgcac gtttgcatcc cgctgcccga aggccccaag 720
tgcgtctgtc ccgcggggta ccacacgctc gacgagaaaa actgcgagga tatcaacgaa 780
tgcgaaacat acggtatatg cgatcaaatg tgccggaaca cgccgggttc gtacgaatgc 840
tactgcgaca aggcgtaccg tttggcggag gacaagaaaa cctgcaaggc ggagggcgag 900
gtgggcatca tggtgttcag ctcgaaggat caaatcagag cggtggcgct ggattcgacc 960
agctacatca tcgtcgccaa gaacttgaag aaagtggtgg gcgtcgattt cgacggtcgc 1020
cgggtctact ggacggagat atacggggga tacgagaaca tcgccaggag cgccgaggac 1080
gggtctcaga aggaggtgtt agtaacggct ggcgtagatc taccagagga tttgtccatc 1140
gattggttga cgggtaacat ttatttcacc gatggcgaga agcagcacat cggagtttgc 1200
tcgccggacg ggacccattg cgctgtttta atcaataagg acatcaggaa acccagaggg 1260
atcgttctta acgtggtgga cggcgacatg tactggacgg attgggaaga tccggcgcga 1320
atcggttact cgctaatgga cggatcaaat gataagcctt tcgtggaaaa agacatacat 1380
tggcccaacg gtttagcatt ggatcatcct aatagtagat tgtactggac ggatgctagg 1440
aagatgacat tggagagcat acgtttagat ggaactgatc gaaggataat attagaagac 1500
atcgtgaagc atccgtacgc catagcagta ttcgaggata aattgtactg gtcggattgg 1560
gcgacgcgaa ccatccaaca gtgcgacaaa ttcgacggga agaaccacaa aacgttgatc 1620
gaagagaaag agctcatcta cgggatcagc atattccatt cggcgcagca gaagcgcagc 1680
aaaaacccct gcgagatggc gctgtgcagc gacttgtgct tactgaaagg tacatcgtac 1740
agctgcgctt gtccacagaa caaggtctta caatccgacg ggcttttttg taaagaatta 1800
tcgcctacag aaactcttat cgccgctcat cgggatatgc tagtacatat agagcatccg 1860
attcttggta ggcatgttgt tacagctcta cccggcgcca ctgcagacat agaaagcatg 1920
acattcgaca gctataagaa tgtactgtac gtcagcgact tcaagaccag gaggatcagt 1980
actttgcaca tgaagaccgg tagtagcaaa gttatggaca tttccgattt gggtagaatc 2040
agcgctatgg atttcgattc taaaagcaac aatctttaca tctgcgattc ggtacgcaaa 2100
gtggtggaag taatcagtct caacacgatg gcaaggcgga ttttaatcca cgacacgttc 2160
ggagaaatcc cccaaagtat cgcgttagtc cccgacgaag gggtgatgtt tgtaagcttc 2220
ggaggcgagg gacccagcca catcgatcgc ttctccatgg acggtacgga tcgaactcac 2280
tctatagaca ccaaactaac ggggccagtg tcactggcgt acgatcccga tttacacagg 2340
gtgttcttcg ccgacgccac caacggctta atcgaatcca ccaacgtgga cggcgacgat 2400
cgcatccact tcagaaccgt cgattctcac cccatcagct tgctggtgct caaagacgat 2460
gtgttctggg tgaacgtgca ctcgaagcag ctctactgga cctcgaagaa agtcccatcg 2520
aattacgaca agaaaattac gctggcgttc cccgacgatc cggacaaggt gcacttggtg 2580
tcagtaacgt cgcgccatgt agagagcaac ttatgtcgca tcaacaacaa cggttgcagc 2640
catctgtgct tgcagtcgca gaagtccatc gtgtgccagt gcccgatcgg ctgggagctg 2700
aaggaggaca accgcacctg cgccaagaga gtgggttgca tcggcggcga gtttttgtgc 2760
caccactcca acacttgcat tttgaaaagc ctcagatgca acggccacaa ggactgcttg 2820
tttggagaag acgagagcga ttgcgcagct gcagcgaaat gcggaggaag cgagttccaa 2880
tgcgccaacg gcgattgcat agctgcggat ctggcctgca accatcggta tgattgcaag 2940
gataaatctg acgagcacgg ttgcgatgat gttaagaacg gcactcgatg cccgccagat 3000
cacttcacct gctcgaacgg ggattgcatc agccagcatt tccactgcga tggcgtctcc 3060
gattgcacag acaactcaga cgagattcac tgccaggtga acgattgtaa tgcaacgcaa 3120
tttaggtgcg attcgggctc ttgcattccc aaggaatggg agtgcgatca cgattacgat 3180
tgcatggata gttctgatga gcattgcaat gcgctgtgcc cggagagcca cttcaaatgc 3240
gacaacggcc tgtgcgtgga caagaagtta atgtgcgacg gtttcgacaa ctgcggcgac 3300
cactccgacg agaagatgca catgtgccac cagagggccg cgcacaactg caccgcctac 3360
gagacggcct gctcgtccaa cgcctccatt tgcattcctt tggaagcgaa atgcgacggg 3420
aaatccgact gtcccaagca cgaagacgag atcggctgcg ccatgtgcgc cgacgaggat 3480
ttcgagtgcc gcaacaagca tttgaaggac tgcattccga ggatgtggct gtgcgacggc 3540
atgaacgatt gcggggacaa cagcgacgag gacttggtga tgtgcagcaa aaagaacaga 3600
accatgttcg ctccggccca agtaccctgc accgatggct tcaggtgcga taacggcaac 3660
tgcatcaacg catccttgct ctgcaacggg aaacacgact gctatgatgg ctctgacgaa 3720
ggaggcttgt gcaccgggtc ctgcgaaggc tctaagaatc cttgtaatca catctgcatc 3780
aaaacaccga aaggtcctaa atgccagtgc aggtcgggct acaagttgat gggcgatggt 3840
aaaacgtgcg ttgatgagaa cgaatgcgag gtctatcctc ccatatgcag ccaaatttgc 3900
cgcaacagag aaggcggtta cacttgcgat tgcttccaca acttttattt aagaaaggac 3960
atgaaatcct gcaaagcggc gggttccgac atgttgatct acttcaacgt gaacggcaat 4020
caaatctacg aaatcgcgcc gaagaacaat tcgatgagcg tcgtccagga agagtcgatg 4080
ctgaagatca cgagtctgga tactctggtg gactctaagg agctcttctt cagcgtgcag 4140
gacaccggcg ccatctacaa gctggatccg gctacaggaa ccatgcacta catcaagaac 4200
ttgggcgagc cgaagctgat agccgtggat tggtcgacgg ggaatgttta ttatcacaac 4260
gcccaatcgg acgcgaaatc gatcggggtg tgcaattacg aggagaagtg cgccaaattg 4320
atcgacatcg acgcgcatcg acaggtgtcc gcattggctg tggattcttt caacaaagtt 4380
ttgttctatg tggtcagatc gtggacgata ttcgcatcgc ccagctacgt tatatacaaa 4440
accgatttgg acggtagcaa cgtggtggaa ctggtcaaaa caaatatagg caacgtggaa 4500
cacatcacct acgatttgaa caaacgacag ctgtacttcc acgacgagaa cacccagcaa 4560
atcaacgtga tcggctacga agggggccgc accaaatccc tcttctacaa cgtgacttta 4620
atcgaagggc tcagactgtt cgaagataat ttgtactact tgcgccagga cgggttcctc 4680
atcagatgcc agttgttcga agaggccgcc tgtcactacg gtttcaactt gcacagtttg 4740
gctagggacg agttcgtaat ctcgcacaga tcccttcagc ccttcgttgg gcacgtctgc 4800
gatgggcatg cttgccccta catgtgcgtg gctggacaag agggctattt gtgcgtttgc 4860
catgacggct cgaccagtgg cgaatgcggc acaccgcaag acgagaacgg cgagaacaaa 4920
ttcaaagtaa cttccatcga gggaaaatcc agatcttcgg cgtttttgtc cggtttcgcg 4980
gtgtttgggg tactagtagc atgcgtcatg attatacttg gggcttatta catgataaag 5040
aggagaagcg gtgataacag taccgatttg agcatcgtgc gatttcaaaa tccgctttac 5100
ggaagacccg tggaggacga gaaacctatt ttggagcccg ggaagcacga gtacgtgaat 5160
tgtttgtacc aacacaaagc ggatggtggc gaaaatagca atactgaacc caaaacgttc 5220
aacgtctgtg atgataattt gtgttaattt attgtttttt tatgttttac gttaattagg 5280
tgcattattt gattgttaat tctatttata acacattatt atatgtatat acaaattgtt 5340
a 5341
<210> 35
<211> 5186
<212> DNA
<213> 稻绿蝽
<400> 35
ctggaacgag tttaatttat tgtttgtcac aacactaatc attctggtca cttctagcga 60
acaacatgag acagaatgcg atgataattt cttcagatgt cgctcaggca gatgtatatc 120
tatgagcttc atttgcgatg gatatgaaga ttgcggccat ggggacatgt cggatgaaga 180
gaattgtcat atgaccacta ccccaggaac gaaaccatgt aataaggatg aataccagtg 240
ccaagatagg ctatgcattc cttcgttatg ggtctgcgac ggtgaagcgg actgtttgga 300
tggcagcgat gagactcatg gatgtactgc ggggacatgt gacggttttc tgtgccacag 360
caagacttgt atccccaaag agtgggaatg cgatggcgta agggattgtc ctgataattc 420
agacgaattg ttctgtacta agaaaccagt gaactcagaa gaatgcagta ttcatgaaaa 480
tggtttcttg tgtcgtgatc aaatgaaatg tattgaattg aaagaagtct gcaccctgat 540
tgtgatgata gttctgatga aggtggcgtg tgcctcgttt caaacaaaac agatacatgt 600
actaatctga aatgttctac taaccaaggc tgcataatat tacctgaggg gcctgtatgt 660
actgaggtgt gcatgaaagg ataccacttc actggtggtt cttgtttcga tatagatgaa 720
tgtttatcat ttggaagttg tgaccaaata tgtatgaaca caattggagg ctacaattgc 780
tcctgtattg aaggctataa atttaatatt gatttgaaca aatgcaaagc taaaggaagt 840
gaaggcatat tatttttcac ttcaaaccaa gaaataagag gatattattt agactctgaa 900
gtctacttta aagtagtaga aaatctacca catgcaacag gagtaacata tgatggtgtt 960
aatgtttatt ggacaacagt agcagatgag gaagaaacaa ttgtcaaagc agctgaaaat 1020
ggtgaaaatc aaaaaataat tgtaacttct ggtattggca gacctgaaga tctggcagtt 1080
gattggatta caaaaaatgt atacttcaca gattctaaat taaagtatgt aggtgtttgt 1140
tccaatgatg gatactattg ccttatgctc cacgaagatc aagtggaaaa accacgtgct 1200
attgtcctgc attcatcaga aggattgatg tattggtcag attggggaac agaacctgcc 1260
attatgcgtt caatataata aaatttataa aagatgacat tcattggcca aatggtctag 1320
ccatcgacca tggaacaaat tatttatatt gggtagatgc taaagaagca agaattgaat 1380
gtgtaaaact agatggaacc gacagaagaa aagtaccatc cactgctgta aaacatccct 1440
tttccattga tatttttgaa gatagaatct tctggagtga ctgggacagt gaccaaatcg 1500
tatcctgcaa taaattttct ggcaaagatt gccatacaat aataaaagaa agagaccata 1560
agatttatgg tatccatgtc taccatccta gtaaaggaag ttattaatcc ttgccttgat 1620
gttcaatgct ctgatatatg cctaatttct ccgaaaaata tggatgatga tttgcaatat 1680
acttgtgctt gtccttatca taaaaaactt agcagagatc atcatacatg tattgaggat 1740
aagccacttc aaaccataat tgtgggttct ggacagcaga tcattaaaat acaacataaa 1800
aaatttggag gatttagttc agatcatttc aaactaacag tattaaggaa aataggtgct 1860
ctagcttatg atccaaattc agatagaatc attgtaagtg acttagttag acgtaaaata 1920
ttttcattca acgttaaaag tttcgaatca gagatattaa ttgaacacaa tattggaaaa 1980
gttgttggta tggatgttga ctattatgaa aataatctat attggataga tgatgagaaa 2040
aaaactattg aagttatgaa tttgcaaaac aaacatcgat taacactcat acgagatcta 2100
aatgaaggtc tcaatgatat agcattggtg tttatgtttg ttgcactttc aacatttgag 2160
ggtgcacaca ttgacaggat atcaatggat gggcgacaaa cttccagaat tcatgtaatt 2220
gaagagaaac tatttggccc tttgtctctt agctatgatc ctaaacttga gcgactgttc 2280
tggtctgatc aaatgggagg tgaaatagct tccacagctg ttgaaggact tgatcgacat 2340
atttttaaag acggacattc cggtccagta gatgttgcag ttactgagtc agaagtattt 2400
tggcttggtt atggagctac gaaggttttt tggtcaaata agtttgatgg atctcttaca 2460
aaaagattta ttttagacaa gcttgaagaa actgataaca tgaaaatcat tggattaaat 2520
aaaggtttaa ggaaggaagc aagtgcttgc aatgcccttg gaaactgcag tcatgtctgt 2580
ttgatttcta cacatatggg gatttgtgca tgccctgacg gaatggagct tggatttgat 2640
agaaaaacct gccaaaaatt acttgcctgt aaagaaggac aatacaaatg taccactggt 2700
gaatgtattt caaagtctct acgatgtaat ggtagatcag attgtaggct tggtgatgat 2760
gaagaatatt gttctataat ttgttcacca gaccagcttg cttgcttaga tggttctagt 2820
tgtattgaaa aatctaaaaa atgtgattct aaagttgact gcaatgacgg ttctgatgaa 2880
aaatattgtg aatctactcg gaattgtact ggaaatgaat tccaatgcac atcaggagaa 2940
tgtgtttcca aagtagcaca atgtgatggc atgaatgact gcaacgatgg cagtgatgaa 3000
agcagttgta atgctttcac ttgtaaaact aatgagttcc gctgcaatac aggaaactgt 3060
ataccaaatt catgggtatg tgacagtcag attgactgta atgatggttc tgatgaaggt 3120
ccaaaatgtt ctgaaattga aggatgcgat gaagatcagt ttacttgtga caatggtcac 3180
tgtatttctc atgttttggt ctgtaaccat gaagatgatt gtgaagattc atctgatgaa 3240
aaatattgtt actttccaaa aagcaaaaaa aaatatgata atgaggaaaa taagactcag 3300
gattcatcaa aagactgcca atttttatgc ccttcagatt taaaaatatg tttgccttca 3360
tcagggagat gtaatggaac ttcagagtgt ccagacggag aagatgagct tcgttgcaac 3420
aagtgctctg aggaagaatt cacttgttct tcgagtaagc gatgtttgcc aatgacatgg 3480
gtttgtgata atgtcagtga ttgtgaagac cagtctgatg aaatgcattg ccttaatgga 3540
accttggctg tacacgatat gtttatttca gacaatcttc tatgtgatgg tttattctgc 3600
aaagatagta agaaatgtat tcccttaagt aaactgtgtg attctcttat ggattgtact 3660
gatgggtcag atgaaaaagg cctttgcggt aagggatgtg aacacgctgc atgtcatcac 3720
aaatgtaaag agacacctat gggacctagg tgtgaatgct taagtggtta tactctagga 3780
ggtgatggta ggacttgcag tgacatcaac gaatgtgaga aaatgtcttc ttgctctcaa 3840
tattgccata atactgttgg cagttatagt tgttcatgca tggatgataa ttttcaacta 3900
agatctgata aaataagatg caaagcaaaa ggaactgaaa tgaagtatta ctttgccacg 3960
aaaaaagaaa ttaaatataa atcccaatcc ttacagcaaa cagttgtagt gtatcaatca 4020
cagcgtgata tggatatgaa aggaatggat tttgatatga ataatgatat aatattttgg 4080
acttcagaga ttagtggtta tttgtacaaa gttgatatta aatctaaaaa catcactcaa 4140
attacaaacc tcataagacc aacaaaaata tcatatgact gggttactgg taatgtttat 4200
gtccttgaaa acttcaaaat tataagagtt tgcaattttg aagctaaact ttgttcagca 4260
ttgtacacag ctaagaatgg tatcaacatt gaaaccattg ctcttgatcc aaaatcgaga 4320
atcatgttct ggtctgaaag taaatggtta ataaatcaaa cacccaatag tacacttcag 4380
ctggcatcaa tgagtgcaga taatccttgg gcagtgctaa gttttgattt tagtccagtt 4440
actgatattg tagttgatca ttttcacaaa gttgtttatt ggtcaaactt agcagaaaat 4500
aaaattgaaa gatgtagtta tgatggaaaa gacagatctg tattattgcg tagtgagtat 4560
cctccaagag acttggtact gtttgaagat tatttatact gggtaaatga tcaaaaacaa 4620
tctcacatta ttcgtccaga agcagaagga actattacta aatatgggct gtatggatct 4680
gtgtatatga agcatgaatc agtaaggctt ttaccttctt catcctacaa tgaaattaaa 4740
gctttccggg tttcacagtc tgcactccag cctgaaggag tgaatcattg tagcaaaatc 4800
acttgtaatt acttgtgcat aagcaatgaa aacgaaccaa tatgttactg tgaagatggc 4860
tcaaaagtga aacccggaga tatatgcaac aatcatactg gaatacattt caaaacctat 4920
gaaacagaaa atgtatcagg gagtagttca gtattagctt ggtttttctg gattggtggg 4980
ttagttatag cagctggact tggttatgca agttatcatt ttgtagctaa ctatgttcaa 5040
aaaaataaaa tacacccaag gttcagtgta ttttttcctg ttggccgttc tgaggctcag 5100
agagaacaag gatcacaggt tcatcagttt gaaaacccta catatagtga ggtcattgtg 5160
gtataatgaa atatgaagtt aattta 5186
<210> 36
<211> 3378
<212> DNA
<213> 玉米根虫
<400> 36
agaatgacgg attgaattga aaaagaaaca gctgaacaag aattaactgt ctacgaatga 60
tgaaagtaaa ttgatgaagt ataaaactta gaaattagat aacagaagta gtcaactgca 120
tccaaattca gctgatttag ggcatttacg ggaacctttt accttttggg aagtgaaagt 180
ggccgtgata tcgaattagt ataaccgaac tgaaaatgac ccatgtagta caaacacaaa 240
aacaagtcat tcgttaattg gtaaaagtgc ctaaggtcct attttcatct tgtaactatg 300
aaggattgtt taaagtgttg ataagttagt aatgtaccct aaaattaatt gtgtgtaatg 360
aatacctgct agtaattacc gaatttggag cgagaaaaga gaaagaaaat gtcatcgata 420
aaaagtggag gtctaaccaa aaaggaaggc aagatgagga tcagagcctt cccaatgact 480
atggatgaaa aatatgtaga aagtatatgg gctttattaa agaatgctat acaagaaatt 540
caaaagaaaa ataactcagg tttaagtttt gaagaattat atcgaaatgc atacaccatg 600
gtattacaca aacatggtga acgcctttat acaggattaa aggaagtggt cacccaacat 660
ttagaagtga aggttagaga agatgtgtta cgatctttac ataataattt ccttatgact 720
ttgaatcaag cttggaatga tcatcagacc tccatggtta tgattagaga tatactcatg 780
tatatggaca gggtctatgt ccaacaaaac gatgttgata atgtatataa tttgggactt 840
atcattttta gagatcaggt ggtacgttat ggctgtatta gagatcatct tcgagagact 900
ttattagata tggtaatgcg agaacgtaga ggagaaaaag ttgatagaat ttcaattaaa 960
aatgcgtgtc aaatgttaat ggtacttgga atcaactccc gagttgtcta tgaagaagac 1020
tttgaacggc cttttttgca acagtctgca gaattttata aggtggaaag tcaacggttt 1080
ctggctgaaa atagcgcatc agtgtatata aacaaagtag aagcgcgaat caatgaggaa 1140
tccgatcgtg cgaaacacta tttagataaa tcaacagaat ctcgaattgt tgaagtagta 1200
gaagaagaac tgatcaaaaa acacatgaag actatcgtag agatggaaaa ctctggtgtg 1260
gtacacatgc tgaagcacca aaagaccgaa gatctatgct gtatgtacaa actgttcggt 1320
cgcgtaacag atggtttgaa aacaatggca gattgcgtca gtttgtatct tagagaacaa 1380
ggtaaagccc tcgtccagga agaagaacac cagccggcca ccaatgctat ctccttcgtg 1440
cagtcattgc tcgatctcaa agacggcttc gaccatttcc tccagaattc ctttaacaat 1500
gataaaatat ttaaacagat gatagcgtct gattttgaac atttcctcaa tttgaatccc 1560
aaatcgccgg agtatttgtc gctgtttatt gatgataagc tcaagaaagg tgtaaaaggc 1620
atgaaatctt ctaaccttga aagattcctc aatttgagcc ccacatcgtc agaatatttg 1680
acgctgttta gtgacgatta cctcaagaaa ggtgtagaaa tcatgaccga acaagagata 1740
gaacaagtat tggataagtc aatggtttta tttaggttcc tccaagagaa agacgttttc 1800
gaaaggtatt acaagcagca tcttgccaaa cggttgttgt tgaataaatc tgtcagtgat 1860
gattatgaaa agaatatgat ctctaagtta aagacggagt gtggatgcca atttacttct 1920
aagttagaag gaatgtttaa agatatgact gtttccaata cgattatgga tgaatttaaa 1980
gaacatgtta cgaagtctga gacaaattta ggtggaatag atttattaat gcgagtgctg 2040
acaactggct tttggcccac acaaaacgca accccaaaat gtcatatacc agctgtacca 2100
ctagctgcct tcgaatgttt taggagattt tatttagcca aacacagcgg ccggcaactt 2160
acgttgcaac ctcaacttgg aagtgccgac ttgaacgcta tcttctacgg cccgaagaaa 2220
gacgatagcg acaaggatgg cgcttgttct tcctcgacaa caatagtctc gatgaggtca 2280
gggcctagga agcacatcat acaagtgtcc acttatcaaa tggtagtttt gatgttgttt 2340
aataatcacg ataagttgac atatgaagag atccttaatg agtctgatat accagaaagg 2400
gatctaatta gggcgttaca gtcgctggca atgggtaaag caacgcaaag agttctcata 2460
aaaaatccca ggaataaaga aatagaatct aatcatgaat tctatgttaa cgaatcgttt 2520
agttctaaac ttcatcgagt gaagattcaa accgtagctg caaagggaga gaacgagccc 2580
gaaagacgag aaactaggaa caaggtcgac gaagatagaa aacacgaaat tgaggcagct 2640
atcgttcgga taatgaaatc gcgtaaacgc atggcgcaca acatcctcgt cacagaagtg 2700
acagaacaac tcaagagccg tttccttcct tctcccgtca taataaagaa aaggatagaa 2760
ggtctcatag agagggagta tctggcccgt acgcccgaag acagaaaagt gtacacatac 2820
gtggcatgaa ctttaacaag gatcattatg agactttgga tagtggtcca gtgatcttca 2880
atagatgaat atttacataa atgtaattat taattattaa attattccaa caggttcttt 2940
ttgtctaatt tcttcaagtt gttgaagaaa aataatgtat ttgtggaaag ataacttccg 3000
gaccatatta acaattttca atttatttat aataattttg tgatagattg attgacaaga 3060
taattttaga ttttcagttt gcaataaata atgtagagtg tgtttgatga tatattaaat 3120
ctcgatttgg acttttcgga aaattaacac gttgatggac agaacgtata ggcgtctaat 3180
ttccattgat gtaatgtgac acaacgtcta ttataatttt taaaataacg agttgtaaca 3240
aaaaatccat gtcagaaaat atcacatttc atctacagag gcacattgtt tccataaatt 3300
aaaagcgtta aaagaaattc agcaattttc ctattctact ttgcaaaata caaggtgatt 3360
cagtgaaacg gtacgatt 3378
<210> 37
<211> 1786
<212> DNA
<213> 玉米根虫
<400> 37
caccggctta cgtaataatt aatttattcc tttcatttgc accatactat atattattta 60
ttgagtggac aaatgttaaa aagtaagtct ggcagcgaac gtgttaatga aacctagtaa 120
aaggctccaa catttgacac ttcttcatgc aacatgttct gttctgtgtc aatctaacct 180
aacctaacat tttcaaatac gaatatggaa acatttcacc acatcaacac gtgaattaat 240
attcgaaaat ggagtacgaa aatacacaac aaaatataaa cttcgtaccg tgtgtaagat 300
gggttaaacg aggagtggcc aattcaagcc cagtaaaatt gcaactgtcg aaaaacgagc 360
tggctcaaat tattaatgac accaagatta aattacaaga atccaatgaa aatgaagatg 420
agcctatgga agaaggtgaa acgtctcaaa cagatgagtt tgccttagag gattacgata 480
aagaagacga aaatgaggac actgcaaatg ctttaggaat tggatcattg gcagaactcg 540
ataatgatgc tgcagacaat ttttctgagt cagacgattc tgaaaaagaa gatgataaaa 600
tcaaaccatc tgacaatctc atactagtag gacatgtaga aggggatgca agtctattgg 660
aagtctacat atacaatgaa caagaagagt cattgtatgt tcatcatgat attatgttat 720
catcctttcc tctgtgttta gaaccgctaa actatgaacc gaagatgccc aaaggaaatt 780
attgtgcagt gggatcaatg tcacctgtta tagaggtctg ggatgtagat attatgaatg 840
ttattgaacc ttcatttact ttagggagac ctgctagtaa aaagaagaac aaggaacata 900
taggccatac ggatgcagtg ctttcattag cttggaacaa aacctttgaa catgtgttgg 960
ctagtggatc tgtagatcaa acaatacact tgtgggatat ggaaatcaaa aaaccaagta 1020
caaccattaa atccttccaa gaaaaagttc aatgtctcga atggcatcct ttagaggcac 1080
aaacactttt aggtggaggt tgtgacaaat ctgcaagagt atttgatggc aggacccctg 1140
aaacccacca aacttggcta cttgatggag aagctgaaag gctatgctgg aacccattag 1200
aacctttcac atttttagca ggtaccagca gtgggtctgt acagtgtttc gactgtagaa 1260
aaggacagct atggtcagtt aaagcacaca gcaaggaagt aacagggtta gtccttagca 1320
aacaatgcca aggattgttg attacttctt ctacagatga aacagttaaa atttgggact 1380
tggctacact ggaagctgaa cctaagcttg ttaatgaaaa ggagtttaat atggggaata 1440
ttcactgttt ggatttatgt ccagacttgc cgtttgttat atctgtaggt ggagataaga 1500
agtcaaataa ttttactgtt tttgatgtac agaatattga tgttgttaaa cacacatttg 1560
ggccaagagg attggtacag ctagttccag atacagaaga aaatactaat acgttttatt 1620
gtttaataaa gtttttattt tatttatttg taataatttc atgcttcttc tttaagtacc 1680
gtgcccaaat attagtcatt ggtagattcc ttgacaattt gccgatactg ttttcgatta 1740
ctgaacaatt catcttctga tgagccagtc cattgacaaa ggtttc 1786
<210> 38
<211> 3948
<212> DNA
<213> 玉米根虫
<220>
<221> 尚未归类的特征
<222> (3773)..(3795)
<223> n是a、c、g、或t
<400> 38
tggttcttac caacttccag acattttttt ttggtggtta aggcccatct aaaatgccca 60
aatcaaataa atctagaaat gcgttttact actttatgga ggattttaaa caaagacaag 120
gatttgcttg taaatcgatg aaggatgtag cagatgctgc aggtccccat tgggctagga 180
tgtctaaagc agaaagaaga ccttatgaag agagggcaaa caaagagaag ggaggtttga 240
gatatacttg ggatggtcaa tgcgttgaag atatagaaag gaggaacaaa gctgaggaca 300
ataaaattgc taagatgaaa gatgatatcg actgcacctt gaaatttgcc aaagaaaatt 360
gtcgactccg agatgaaatg ttcttcataa tccatatcaa tactttctgt taccatgcca 420
gtggtgatcg gtattatccc gctgaaattg ccatttcatg cttcagcttg gaggatggcg 480
ttttgcctca aaatgtgttt cataaaatta ttaagccagg tagactacca ctggggtatg 540
ctagtgacgc aaaaaagcaa tctgaagatt cacatcaact gcctatcccc ttaactggtg 600
acgacgacga caatgtggaa gaagtttata cagagatgaa agaatttata ttatctaaaa 660
ccggagatac taagaaattc cctcccttgt atgctaagaa agatattgtt aaaatgttgc 720
aacatgtgtt agacacttgg tgcgctgatt ttgatgagcc gccattattt aaagtttatt 780
atttacaata tatgtttcaa gtattaaaaa actcggtagc taacgataac gtatggccct 840
tatattcaat ggcagaaacg gaactggata aggatcttta ttcccatact gttggaatat 900
gttgtgaatt ccattctgtt agtgaagctt gtcaattttg cagcaagtca gtggcagtac 960
gacttgcata cacaatttgt gataattgct gttcgttcct agatattcct atagagcctg 1020
gaagacacgt acctgatcaa gcttttacag catccagttt gagctcaaga gcttcatcaa 1080
aagctagttt gtatacaggt tccagtaaat cttctaagaa gcagtcagtt cgtgaagatg 1140
acaaagacac tgttttatcg ttttctagtg cctctcagtg ggagagcgaa tctgtagtgt 1200
cagactcctc tacagctact ttcaataata gctttccagg tctgaaatcg aaatggacat 1260
taagcaattc tcaacctgta gaaactgact caatgagcca aatgtcaggt cagagctatg 1320
caagtgctat gggaactaga agacagtttt ctagtgcaat gggaaataga ggatctcaaa 1380
gagagtcctc taacaattcc aacaatggtg acactagtga ttcgaatacc gattatttta 1440
acagtcaatc atttccggct cttggaaggg gaagaggtat ggccagaaat cgaagaaatt 1500
gatcattttt gactgcaatg ggtcttgttc cacttcatga gattgttaaa tgagtatgta 1560
actgattaca ttaggcgaca atattttttt tagtgtcaga ttatttgatg taattgtcct 1620
tttgacaatt ctttaatcat tgatgttttc ttctattgtg gtgtttttag atgtcatatt 1680
tttatgtatg taaattggtt ttttatctaa gtatagaata ttacatttta gtttaataag 1740
ataccagtaa agtatattgg tttaccagtc gcctaatgta aactagtata tacataccta 1800
tattattttt ctttaatagt tttaagtcaa ttttgataca gatcacgaga cagatatttt 1860
ttccgtctag tccgaattgc gttccgtttc ttgggccttg tcgaattgcg tcccaaatat 1920
tttgcttacc tggccaccga taacgtctag tccgaattgc gtttcatttc ttaattagcc 1980
aacccaattg cggtcagaag atagcttaat aaatattagt taagcaggga aatacaatca 2040
acgtaatatc aactaacagt ttatttttat tttagcaccg aatttataac taacacattt 2100
aacgaaattg tatcttgaaa tttcacccct gttatactgg ttaattttct gttcgagaaa 2160
aatatatttg ctggaggtag ctttatctac atttctagaa atattttgtt taacagaatt 2220
tatttttata taagaatcgg tttgaatgcg ctttaatgtt tcaacaaaca aaaaaatatt 2280
aggatgtgga gacaaacaat aatttttaaa ttttgaatga aatcactcac aagaattagt 2340
tgttctatat atagatgcgc tattctttgc ccaaatttgt ggaggaaatg tgtagtcctc 2400
ttcgatatac ctttcaacta gatagtttgc aaatgcgtca actcgatgat catctggttt 2460
ttcaggcata aaatcttcca caaaacaatc tccaacttgg ctcggaggaa gatacattag 2520
gccaaaaata tgcttcagcc atttacctat ttctgagttc tcgttcttat actcagaact 2580
caaccccata ttttgtactt tacgccacct gtaacaagat tatagaataa attctttatt 2640
tcagaaagta cttgcattca agaaaaagtt tgaaacaggt cagattttat aaaaaaaata 2700
catctttaaa aaataatgag aatatttagg agccaatttt ttgacagagg ttatgacgac 2760
atcttttttt tatgtcaaaa atttgaagat attttaccta caattcttgt tttaacttat 2820
atcaaatgct gttttttgta aaaaaaaatt gaaaaaagaa attacagtga agtttgaaat 2880
aaacatattg aaatgtttag ttttacagca aaaaaaatag gatgacgtca taactcaagt 2940
ttttcttaac aatctactat ttgccagtta cattgccata cgtattactt accaagcttg 3000
agcaagatga agtcgataac caacaacatt tgcttccgga aaaacttctc gtacgctgga 3060
gtggatacct aattcgtaat caatcactat tttcgttgga tttaaattgc atcccaaatc 3120
ttgacatttt ttctttaata attcgaataa tgatttgtaa gtgaattttt gcttattagg 3180
taacaaacaa aatgcaaccg gaacataagt gtcattaatc aatgtatgta atgtaaacat 3240
ttaaaaaaaa attgtgcaat aatcgaatgt tccatccata aataaagtgg aattgttaca 3300
cataaatgaa agattagaca ttcatgaaaa aacaaaaata ttaaactgtt catcattaat 3360
caacaaaaac tgttcatttt tgatagtaaa tatttcaatt tgttccaaaa attcttgaac 3420
atccttggct gattttggca attttggaac cgttttcaat cgcgtgcgat taatgttctt 3480
tttttatgta ttggacatct tttgttgaca gagaggtaag tacttcgctt tgcttttcga 3540
tttccttgtg taacaacttc gcaggtcgtt ccgcaatatc ttctgttgct tttcttttaa 3600
gagaattgtt aactttctgg cgaattaata cttcttcagc aagctcatga ttgtgttcac 3660
cactaacttt tgaaaacaca ttatttaatg tatatagttt tgctttacac tttttgaagt 3720
tatacttctt taggcgcgat tgagtaatta tttattatta atctgcgcgc atnnnnnnnn 3780
nnnnnnnnnn nnnnnggttg ctaatctttc aaattatgta tcagcgcaaa taagccatta 3840
aaataatata ttagtgtttt ttagtaaatg tattatttat tatcattttt gtgtctttgg 3900
atttgttttc ctttggcgta aaataataaa atatctattt ttatattt 3948
<210> 39
<211> 2088
<212> DNA
<213> 玉米根虫
<400> 39
caatcaagtg tcaaattgtt tttaaaaaat aatgacagag gaatcagtta cagcaatttc 60
cgaacgagaa gaactagaac cagaacttcc cccaccacca ccgataactc tgaaggaagt 120
ctttgtacaa gaacatgaag atagtcctga ttcgtctatt tccgggtccg atgaagaaag 180
aaaaacagac aaacccagag ctgatatacc tcccgaatac tggcacatac aaaaattgat 240
taaatattta aaaactggca atcaaacggc tacagtggtt gcattatgtt gtttaagaga 300
ccatgactta actacagaaa ccagtcagct tgcaatccaa gaaataggcg gcctggaaat 360
attaataaac ctgctagaga caaaagacct caaatgcaaa ctaggtgcct tgggcgtttt 420
atcagagctg tctacaaata tagatatacg caaatccata gccgatttgg gtggagtgga 480
gttattggta cagaatttag ctgagccagc tagagatctt caagtacttg tagccgaaac 540
tatatacaat gttgctcaaa tccgaaaagc taggaaacac attcgaaagt gtaatggaat 600
acctaggttg gtcgactttt tagatgtgaa ggagtctcta ttgaagacgc cgaaagatca 660
gttgaatgag tatgatgctg aacaggtaaa catagcaaaa gctgccacca gagccttatg 720
gtccgtttct aaaagtaaga agaatattcg cgtaatgatg aaaagtggtt ccgtgcccct 780
actagccaga ctgcttaaat cagaccatat ggatgtggtg gtaccaacta tcggaacgat 840
ctcccaatgt gccatcgatc ccacttatca attggccatc cagactgaag gaatgattaa 900
ggatatagtt ttgcatctat tttctgagga atatccagct ctcagaagat actgctcaga 960
aactatattt agatgtggag aaaatgctct gattagagat atggtaagag aagctggtgg 1020
tctggatcct ttgatcaaga tggtaaggga tccgaaaaca aaggaagaca aaccgctatt 1080
ggcggcagtg actggagcta tctggaaact ggctataagt ccgcagaatg tagaacgctt 1140
cgatcagtta aaaacggttg agagcctagt aacattattg gaaaacgtcg aggaagacga 1200
gggcgttctt agcaacgttg ttggtgctct tggagaatgc ctaaagtttg aacacaatcg 1260
acacaacctg cttagagcta atggtatacc tcatttagta aatctgctaa attataccta 1320
tccccctctt ctcgaaaacg ttccaatggt tttaagggaa tgcgctgaag attatgactc 1380
gatgcgtgtt atagaagaac tggacggagt aaggctgatt tggtcattgt taagaaatga 1440
ctcgcagaag gttcaagcta acgcggcttg gtcccttgtt ccttgtgtca gatacgccac 1500
agactcagga gaaatggttc gctgcttcgt tggaggatta gaactcatcg tgaatctatt 1560
gaaatccaac gataaccacg ttctagcatg cgtgtgtgcg gctgtttctg aggtagctaa 1620
agacatcgaa aatctggcag ttatgaccga ccatggggta gttccgctat tggttaatct 1680
ggtgaataca gatgatgtgg agttgagaca gcatttggct tcagctattg cttattgctg 1740
tgcgtgggga accaactgca aaatgtttgg aagattgggt gccattactc ctctagttca 1800
atacatggcc gacgatgacg ccaatgtcca cagaaccaca gctctagcct tgttccacct 1860
ttctaagaat ccgttcaatt gcattacgat gcacgaaagc ggagtagtga cttttcttct 1920
aagagcagtg tcttccaaag actgggagct acaagaagct gctgcggggt gccttgctaa 1980
tatcaggaaa ttggcattgg aagctgaaac tgtgcatctt attagaaata gggacgacac 2040
ttctgatgag gacaattagg gatacgcttt ttgtggattt ttatatac 2088
<210> 40
<211> 2198
<212> DNA
<213> 玉米根虫
<400> 40
cgaactaagc taaaattaat cgtgaagtga aatccatttt aagaaaaaaa tggtttaata 60
agaataattt atacaaaagt gatatttata tattttcgtt gacgcgttga ggatattgtg 120
aaatatatca aatagtgtca acatttgaac aaatgactat atatgtgtaa aagactgtag 180
tgatcaccag atccaaaaaa gggtgtcttc aagaaatgtg ttaaacccat gaatctttag 240
aaaagagtta aattgaagat atgagtggcc gaccaagaac tacttctttt gcagagggca 300
acaagcaagc taagaatcca cctttggggg gtatgaaaat cagcaataaa atttaaaatg 360
gcgtcgaaaa gtaaaagagg aaaaagtaaa gaaatggaag atgatattag taaacttgta 420
tctacactca aagttaggcc aggtaaggat ggaagcaaag tgactacagt agttgcgacg 480
cccggccaag gaccagacag acctcaggag gtatcataca ctgacaccaa agttatcggc 540
aatggcagct tcggtgtggt ctatcaggcg aaattgtgcg aaacttcaga attggtagcg 600
attaagaaag tactgcagga caaacggttt aagaatcggg agttacaaat catgcgaaaa 660
ttagaacatt gtaatatagt gaaacttaaa tatttctttt actctagtgg agataagaaa 720
gacgaggtgt acctgaattt ggttttggaa tacatccctg agacggtata taaagttgca 780
cgacactaca gcaaatcgaa gcaaactata ccaattagct tcatcaagtt atacatgtac 840
cagttgtttc ggtcactagc atacattcac tctttaggga tatgccatag ggacataaaa 900
ccgcaaaact tgttgttaga tccagagact ggagtgttaa aattgtgtga tttcggaagt 960
gcaaaacacc tcgtcaaagg tgaacctaac gtagcttata tttgcagtag atattatagg 1020
gcaccggagt taatttttgg tgctatcgat tacaccacga aaattgacgt gtggagtgct 1080
ggctgtgttt tagcagagct tttattaggt ctgcccatat ttcctggtga ttcaggcgta 1140
gaccaattag tcgagattat caaggtgttg ggaacaccta ccaaagaaca gatcaaggaa 1200
atgaatccca actacaccga gttcaagttt cctcaaatca aatcacaccc ttggcaacag 1260
gtattcagag ctagaacacc tcctgaagca attgaactag tagcaagatt attagagtat 1320
accccttctt ctagaattag tccgttacag gcttgtgcac attctttttt caatgaactt 1380
agagaaccaa atactcgact tccgaatgga aacaagctac cacctttatt taattttacc 1440
gaacaagagt taagcattca accagcattg aacagtatat tgttgccgag aggaacgcag 1500
gaggcagcta atcctcagga gagtacgtca gctgctagtc aggaagcctc cgaggcgaca 1560
gcaactatcc aagcagcggc tgcttctggt atagcttagt taatagttag tttattcgtg 1620
ctagaatgtt taaatttaca cttgtcaaat ataataccta aaatacttca ctctactgta 1680
ggggtcgacc catttatgtt tatttgtttg tcgatttaga taatttcaag gctgatgtat 1740
cactttatag gtctattggc caccatttga tatgttttag tttaaataat cgatatacac 1800
atatggcttg gtttgaaaac acctatataa tctttatata cagaagcata tatagtatat 1860
atagtttaag tcaaatttat cactgtaaaa ttcttgtaat tggggcaatc tagttaaata 1920
aatttatcaa gttagttaat gtatttgtaa ttctttagat tcagtatata ggccagcagt 1980
ttacctcgca aaagatgcct agcaataaaa tacagtgata ctactaataa ctgacaaaat 2040
aaagcaaaag atggaaaaca taaaagttgt gagataaaaa gaaaagaaac tagcagagat 2100
ggtaaattta gttataaaaa cctataaact tgaattctac ttaatgtttc ccaccttaga 2160
catatcagag ctatatgtca actaaaactg tcactgtt 2198
<210> 41
<211> 3910
<212> DNA
<213> 玉米根虫
<400> 41
tttagcaaca tttacacggc ggcggtagtt aggaagcaag cgctcgaagc gtcgcaatat 60
agaacgtcaa taacgacgac gacggatagc tagtgcaaca ggtgattccg cgtatttctg 120
ttagttagca gcgagcgaga aattacacaa cgcggtgaaa agggaaaagg ggaggtcgca 180
acacaacgtg ttaatacttt tccttagaag attcgaacga ttcgttttta gtgccgtttc 240
acttcaaaaa tgttttagga aaaaaagtgc gtttaagtgt catgtgtgca gtttattgtt 300
gaatgtcccc ttacagtaga taccggcctt taccaggccc tgatggattg gttctgcaaa 360
catcacccgg gtgtagacct tcaccgacca gtgcttgcag atttttgcgg tggatatcca 420
aattgagtcc ccagcccgtt aataaacaag gattaggggt cctggacatg accgccacgg 480
agaacaccat ccgttttagt gtccataccc tggacaaagc aaccaaagca aaagttactt 540
tagaaaatta ttacagtaat cttatagccc aacatgtaga acgaaagcag aggttagcaa 600
aattagaaga atccttaaaa gacgacagtc tctctgagga gcagaaacat gaaaagaggc 660
tacaacatgc ccaaaaagaa accgaatttc ttaggttaaa acgatctcga ctaggcgttg 720
aagacttcga acccctcaaa gtgataggta gaggggcctt cggagaagtt agattagtgc 780
agaagaaaga tactggccac gtttatgcga tgaaaattct caggaaagca gacatgttag 840
aaaaagaaca ggtggcgcat gtgagagcag aacgggatat tttagtagaa gccgatcatc 900
agtgggtagt aaaaatgtat tatagtttcc aagatcccat taatctatat ttaataatgg 960
aatttctacc gggaggtgat atgatgaccc tactcatgaa aaaagacacc ctttctgaag 1020
atagtacgca gttttatata acagaaacag cactagccat tgattcgatt cataaactcg 1080
gtttcattca tagagatatc aaaccggata atttattgtt agacgccaaa ggacatttga 1140
agttgtctga tttcggatta tgcactggtt taaagaaatc ccatcggacg gatttttata 1200
gggacttaag tcaagcaaag ccttcggatt ttattattac ctcaagtccg atggatagta 1260
aacgcagagc ggaaagctgg aagaaaaata ggagagccct ggcctatagt acagtaggca 1320
cacctgatta catagcacca gaagtattca tgcaaacggg atacggacct gcttgcgatt 1380
ggtggtcact aggtgttatt atgtatgaaa tgttgatagg ttatcctccg ttctgttcgg 1440
aaaaccctca agatacatac acaaaagtga tgaattggag ggatacgttg gttttccccg 1500
ctgaagtacc gatctctgaa gaggccaaag atacaatcat taaattctgt tgtgaagcag 1560
acagaagact gggatctcaa aaaggtctag aagacctaaa attaaatcag tttttccgag 1620
gcgtcgattg ggagcatata agggaaaggc cagcagccat accagtagaa gttaagtcaa 1680
ttgatgacac atcaaacttt gatgattttc ctgatgttaa acttgaaatt ccttctgcgc 1740
cccttcctca agacggcgag atcaattata aagattgggt cttcatcaac tacacattca 1800
aaagattcga gggactcacc cagagaggta ctccaacgaa gaaatagttg tttgttaata 1860
gtttttacca cgataaagat atactaggca gttgaatgcc cagcacttat ttttagaaaa 1920
taaagtcatt taagagatgt ttatattaat tatattaaat aaaaagtagt aagtttcggt 1980
aaagacaaat tccacaatat ctttacatgc catcgatcat tccgtttatt attaattttt 2040
atttctttat tatttgattt ttaaaactat gctatttgtt tcatcgtgtt cttttaattt 2100
ttaactattg caccatcaac tcaaacggtt tatacaaggt tggaaatcca agacatgaat 2160
gaatatacag tggataaatt ttgccacact gcaagtgtag tctacttcta ctcatttctt 2220
tctgataaag tcactacagc ggttttaaag agtttggtgt catttgctct ataaataacc 2280
aaatatgttt tgtaagttgt gttcgtatag tgaaatttaa cattattttt cctatatcaa 2340
taaactatat tctttctaat ctgctctaca tttgagacaa ataatttttt ggtcatttta 2400
ttgcaaattg gattcttatt aaatatctta cttatgtttt tataaaacac gacataccgt 2460
ttttgattac acgtaaatcc tgaagtaata ctattaactg aaaaaaaaac tcgaaatcga 2520
taaaatgcct tgtattgtat ctgctttttt tatctccgta acattcttaa cgatgttcca 2580
tttgaatcct tgattccagt tcttcacgtt ccaatactgt ttcaattttt tatttactga 2640
ttttaattta tcaattaaat tatctttaaa ttaactgctt aatcaataat gtatattagg 2700
tctctctaat ttaagattaa ataaaatata ttagataaat agagtatatt actaaaattg 2760
aaaatacaaa aggaatttct tagcttcata gatatattat taataatttt aaaaaataca 2820
ttaaacagtt gaagttactg actcgttagc aaactaaata aaaataaaac atgtggaatt 2880
tgtttgtgcc gaaatcgtta aatgttctat cgattgcgct gtattgtttt ctaaaaataa 2940
gctattttgt gtattatagg tctgaagcag ttttgcactg ttttgctttt ttaagagctg 3000
tgaaaactgt atcttgcatt gttctcgacg ctatacttct agaagtatcg tacacttcaa 3060
aattatttaa ttcattataa gcacttttcc tgtacaagac ttacatcgtt tcgtcaaata 3120
ttaaatatac attacgctta cacgattaac aattttacat tttaacttat gtgatcagtt 3180
atgttacaat ttttattttt tacaataata gatttttatt tcctattttt taatgtaaag 3240
atctgctttt tatatttaaa cattattaaa gatttttggt tttatacttt cattctattt 3300
aaatgagaaa tttgatgtga ttatcaaaat gatcgaaatg gtaatttgaa aaaaactaat 3360
atttttgatt cggattatta caagagaact gacaacttta tcattaaaaa gagtacctaa 3420
ataattgtac tttctcgctt tttctcactc gttagaagca tagatttggt tttgtttatg 3480
tttagtgtag tccatattcc tcactatctt ccatgactgt attgatacgc gcctgtaggt 3540
atttgaagtg ttccgactat catcactgta tcatctgcat atctcagatt gttaactcgg 3600
cttccgttaa ttttaacgct atatatattc ttgaccactc aatgttttat tcattcctcc 3660
gagttcatat tgaacaggat tgcgaaagca cacaactctg acgaaatccc ttctgtgttt 3720
ctatattttc agttgaattt tcttcttttt gtactaattt tagagttctc gaagtctaga 3780
gttttcctgt atcatttatc tgattacttt cacattgatt atcgtaaatg agccataata 3840
aataaatatc cacaaggaaa aagttttcct tgtatggggc tgtgggtttt cctgtagttg 3900
gctgtaatcc 3910
<210> 42
<211> 2386
<212> DNA
<213> 玉米根虫
<400> 42
aaattttgac attgcatgat agcatgtgaa actatcatta gctcataatg tttgtcagtt 60
tttgactttg acaagagttg acaatctaaa aggtgatcaa agaaagattt taataaaagg 120
ttaaaaacac atttgttctt tgtaagcttt gttcttaaat tttgttaaaa aatgtcagaa 180
aatagagaag aaattttagc tgattttcaa gcttgcactg gaattgaaga tgttgctgaa 240
gctatttatc acctggatga ggccaactgg gacttattgc gtgctgtgag tagagtaatg 300
ccaccagata ctcaaagttt tcgtccacca gctgtagagc ctgtagatcc agatgtcatg 360
atagtagaag atgatgctga tataccaagt gcagtaactg cagacgcagc atcagatatg 420
gtagtagtag gcaacagttt gggccattcg tctacagtaa cgaaaaaagc aaatacacct 480
gatgacagct ttccatccac ttctgctgga aaaagtggat cacgaatgtt aaaatttaat 540
gtgcagtact gtgatagagt tgtcaaagtt gaattaccag agtctgcaac cgttagtgat 600
ataaacatta atcttttaga ctgtaatgaa gaattcgtgg aagagtataa aagtatcatg 660
aatgatctga aactaaagtt gcaccgagaa gtgaaagtaa atccttgcag acagcttctt 720
tccagttggg ctcgattagc acagtatgaa gatactccgt tttcgaagct catgttacca 780
aatgacaatt cgcttcattt aacagtaaaa ccaagcgatg gagctttcac ctcagacgaa 840
gaaaatgccg tatctgaaaa attaagaggt acttacactc taaacgtaaa atatgagaat 900
aagacattta atttaaaata tcaaggaaca aaaactattt tggaagtaaa aggtgacgtt 960
tacacgttaa cgaatataca tgtgcgtaat caagtatgga gtgggtggcc tcctaatata 1020
gacgacaata cagtactcgc actcagtgga ataaactatc ctgaacatga tctatcagtg 1080
cgacgaagca cacagagtgt tagagagaaa aaaagtatgc ccgtggttca aattgacagt 1140
gacgatgacg aatttgaaga tgcctccgaa agttttacag ttgatgatga gtattttgtt 1200
gacaatgaag gagcaaatgc aaaaagaaca gacccattaa ttccagagga tgtagaagat 1260
gaaattgtag gctgtataac atttgcagaa cagtttacga caagatacgg tcctgtccat 1320
cctgccttct atcagggaac attagacgat gctatcaaag aagcatgctc aaaacctgcc 1380
aaagaccgaa aaattttggc tatatactta caccacgacg ctagtgttct tacaaatgtt 1440
ttttgcacac aactgttagg ttttgaaagt gtaatgcaaa tgttcgaaaa caactttgtc 1500
ctttggggtt gggatattac ttttgaaagt aatagacgca ggttgcagca atcggttacg 1560
acatctttgg gtgctacagc agcaatgagt ttacgaaata taccagtaga tagattacct 1620
gtcattatac ttataacaaa aattcgatcg acaattgaga tttttagtgt agtgtatgga 1680
aatgttggtg taaatgagct tttgtcaagt ctgatagaat gtgtagaagt atttttagaa 1740
catcaacgag tagaaataaa ggaagaggag gaaagagccg aaagagaaat ggttaaatta 1800
gagcaagatt tagcatatcg tgaaagttta gaagcagata gagcaaaaga agaggccaag 1860
cggttacaag agaaaaaaga aactgaaacc aggaaacgac tggaaagtga aaaggcactc 1920
tcggaagcaa aaaaagaggc ctttaggaaa caagtcgaag cagatttacc tcccgagcca 1980
tctctagaac aaggcgacag tattactaaa atacgattta ggttgccaaa aggagaacat 2040
ttggaaaggc ggtttcagac gactacacct ttgaaagtgc tgttagactt tttaatagtg 2100
aaaggatatc ctacggaaga attcaaagtt atatctagct ggcctaggag agacttgact 2160
tcattagata aaagccaaac actgaaagaa ctgaagctat atcctcaaga aactgtgata 2220
ttagaagagc gatagttttg atcacacatt ccccaaccaa gggttaggtt tttataccag 2280
catttaagcg ttgtaaagtt atttaatata tttttaattt cacgtagtgt atgattaatt 2340
taaatgtatg cagtccgatg aatatacatt acataaaaaa gaaaaa 2386
<210> 43
<211> 2657
<212> DNA
<213> 玉米根虫
<400> 43
atcgtctgtc gtgtttgtca ttttgttccc gcataaacct taacgttctt gttgtcatgg 60
ttcatgtgtg atgaccgtta aaaatttgcc gggatatttt gcttaagttc ggccggaagt 120
gattgattat ttatatattt tacaaacgat tatagttcta cataatggag acaattcaaa 180
acgaaaacaa ggagaatttc gtacatgaaa tgcaaaagca aagcgccatg gccgttcctc 240
aaaaacgacc attgggccag aggaatacca acgtggatcc gcaaccagtt caattgaaag 300
aaaaacaacc acttacagta cccagttcat ttaatgcatt taaagaacaa tttgaaagga 360
acaaatataa aggaacaaaa aatacaaata aagaaaatta ttgccaaaaa ggaaataaga 420
acatagttaa tccagttata ccctgcaacc agtttaaaga ctttaaagta ttcgaagatg 480
aacagtatcc ggaagaaaaa cttaaaaatc ttgcggctaa aaatccaccc aaagtacaag 540
tacacgaaga taccaaggaa gagacaaaag atgaagtgaa agcgccagaa aagatagtag 600
aaaaggaaaa tataaagcaa gattcggaat cactcagtct aaagacatcc gatgcacatt 660
tattcatgac tatagatgat aattataaaa aagaccatga aattccgtca acaaaacgta 720
tgaaagatat tttctttgaa atggaagaat acagatctga tatttatcac tatctaagag 780
aacatgagtt acgaaatcga ccaaaacctg gatatatgaa gaaacaacca gacgtttcat 840
ctaatatgag gacaatattg gtagattggt tagtagaagt agctgaagag tacaaattac 900
acacagaaac cctgtatctt gcagtaaatt atatagaccg atttttgagc tatatgtccg 960
ttgtaagggc caagttgcaa ttggttggaa ctgcagcaat gtttatcgct tcgaaatacg 1020
aggaaatata tccaccagag atcagcgaat ttgtatatat tactgacgac acttacacga 1080
aaaaacaagt actacgaatg gagcagttga ttttgaaagt tttaagtttc gatctctcaa 1140
tacctactcc actaacattt atcacagcaa tgtgtgttag caacaatttg agtgataaaa 1200
ctatgtatct tgccatgtat ttgagcgagt tggcgttact taacggtgac atctacctgg 1260
agttcttacc atcagttata gccgccgcag caatagcgct tgcccgccat actttatgcg 1320
aagaagcttg gcctaaagaa ctcgttcaat ctacaggcta cgaaggtctt caaatcagat 1380
ctggcatctc gtttttatac cagatgtttg tttccgctat gactttacct caacacgcca 1440
tacaagacaa atacaagacc aggaaatgta tgcacgtatc tactttgaaa cctcgcgatg 1500
tggcgttaga gttataattt aattagtaat atttgtaata ggcttaagtc tgccaagggg 1560
atcgaaaatt ttatggctag tttaatcgaa aaaggttcca tgccccgact tcgaagaggc 1620
aataaatgct cgaatgtcaa tggtgcgcgg atgcgctgtt gccaaatcta cggaatgaaa 1680
ttaaattttg ttctatcttg tattaataaa taaaacacgg ttgcatatga catatatgaa 1740
atgaaattac aaacaaaata gttgtagatt tcggtccttg gcaaatgaca agtattgata 1800
attaccgact gaatattatt taatcaacat taataattta tcgtaaattt atttagttca 1860
ataattacaa gacatgatta aaagacgatt agaatcataa tcaaaccaaa ttacacttcg 1920
tcaagaatag taatgtactt atcattgact tgacttcgtc aataatagta aaggattttt 1980
aataaattca tatgcctacc ccccttttaa acttttaaag aaaatttaag ttcattccgt 2040
aaacttggca acagcgcatc tgagcgcaat tagcttttga gtattcattg cttcttctag 2100
cttggggcat ggaacctttt tggattaaaa ctgcaataaa attttccacc cctcttggtg 2160
gacgttaacc tttgtaatat atttttatat ttagtttaat ttttaaggga caatttttac 2220
tgaaatgtga cgttccgttt tacagtattt ttaagagctt tttcctattt gttcgactga 2280
atttacacaa cattatacat tgccttttaa aagtttttct gtaacatttt ttagattttt 2340
tcaacgaagt atgtggtcca agcatgagta aagggtgttc caaaatttac gaaagattta 2400
aatttaatag gaaacgctgt tttgtttttc tttagattgt aattttttat tgcatcataa 2460
agtacgtaga ggtggtgtga atttttgttg aatctcaccc caacagtcgc gaaaggggtt 2520
taattattac cttttgccta attagacatg ggtgaatatt ttacgactac acgcatctgt 2580
cccaaagcag agagtccttc gatattaaca aactagtgaa cttttagggc gactggttct 2640
gagaatttat ttggtcg 2657
<210> 44
<211> 155
<212> DNA
<213> 玉米根虫
<400> 44
tgtcatcgat aaaaagtgga ggtctaacca aaaaggaagg caagatgagg atcagagcct 60
tcccaatgac tatggatgaa aaatatgtag aaagtatatg ggctttatta aagaatgcta 120
tacaagaaat tcaaaagaaa aataactcag gttta 155
<210> 45
<211> 155
<212> DNA
<213> 玉米根虫
<400> 45
cgattctgaa aaagaagatg ataaaatcaa accatctgac aatctcatac tagtaggaca 60
tgtagaaggg gatgcaagtc tattggaagt ctacatatac aatgaacaag aagagtcatt 120
gtatgttcat catgatatta tgttatcatc ctttc 155
<210> 46
<211> 155
<212> DNA
<213> 玉米根虫
<400> 46
atggaggatt ttaaacaaag acaaggattt gcttgtaaat cgatgaagga tgtagcagat 60
gctgcaggtc cccattgggc taggatgtct aaagcagaaa gaagacctta tgaagagagg 120
gcaaacaaag agaagggagg tttgagatat acttg 155
<210> 47
<211> 275
<212> DNA
<213> 玉米根虫
<400> 47
tgatgaaaag tggttccgtg cccctactag ccagactgct taaatcagac cacatggatg 60
tggtggtacc aactatcgga acgatctccc aatgtgccat cgatcccact tatcaattgg 120
ctatccagac tgaaggaatg attaaggata tagttttgca tctattttct gaggaatatc 180
cagctctcag aagatactgc tcagaaacta tatttagatg tggagaaaat gctctgatta 240
gagatatggt aagagaagct ggtggtctgg atcct 275
<210> 48
<211> 275
<212> DNA
<213> 玉米根虫
<400> 48
tgcgaaaatt agaacattgt aatatagtga aacttaaata tttcttttac tctagtggag 60
ataagaaaga cgaggtgtac ctgaatttgg ttttggaata catccctgag acggtatata 120
aagttgcacg acactacagc aaatcgaagc aaactatacc aattagcttc atcaagttat 180
acatgtacca gttgtttcgg tcactagcat acattcactc tttagggata tgccataggg 240
acataaaacc gcaaaacttg ttgttagatc cagag 275
<210> 49
<211> 275
<212> DNA
<213> 玉米根虫
<400> 49
tgaaaattct caggaaagca gacatgttag aaaaagaaca ggtggcgcat gtgagagcag 60
aacgggatat tttagtagaa gccgatcatc agtgggtagt aaaaatgtat tatagtttcc 120
aagatcccat taatctatat ttaataatgg aatttctacc gggaggtgat atgatgaccc 180
tactcatgaa aaaagacacc ctttctgaag atagtacgca gttttatata acagaaacag 240
cactagccat tgattcgatt cataaactcg gtttc 275
<210> 50
<211> 276
<212> DNA
<213> 玉米根虫
<400> 50
atgacgaatt tgaagatgcc tccgaaagtt tttacagttg atgatgagta ttttgttgac 60
aatgaaggag caaatgcaaa aagaacagac ccattaattc cagaggatgt agaagatgaa 120
attgtaggct gtataacatt tgcagaacag tttacgacaa gatacggtcc tgtccatcct 180
gccttctatc agggaacatt agacgatgct atcaaagaag catgctcaaa acctgccaaa 240
gaccgaaaaa ttttggctat atacttacac cacgac 276
<210> 51
<211> 275
<212> DNA
<213> 玉米根虫
<400> 51
tcggggcatg gaaccttttt cgattaaact agccataaaa ttttcgatcc ccttggcaga 60
cttaagccta ttacaaatat tactaattaa attataactc taacgccaca tcgcgaggct 120
tcaaagtaga tacgtgcata catttcctgg tcttgtattt gtcttgtatg gcgtgttgag 180
gtaaagtcat agcggaaaca aacatctggt ataaaaacga gatgccagat ctgatttgaa 240
gaccttcgta gcctgtagat tgaacgagtt cttta 275
<210> 52
<211> 5385
<212> DNA
<213> 草地贪夜蛾
<400> 52
atgaagtatc aaagcttggt attgatagtt agtgtggcgt ggtgctcggc tcagctcacg 60
gatgacatgc agatgtttga gccggagtgc atgacggagg ataagttccc ctgcatggga 120
ggagggtgta tctcggtgtc tcagtactgt gacgggaacc tggactgtga agatggcagt 180
gacgagaatt tttgcatcga acacaagccg ttccaagagt tctgtaacga gactcatcag 240
tacatgtgtc aggactccac gaagtgtgtt cctctgtcct ggctgtgcaa caacgagccg 300
gactgtgatg acggcagcga tgagttcaac tgcaccgcgc tgccggctgt caatgttaat 360
tcaacatgca aaggcttcca atgtggagac ggcaagtgta tctctttcct gtgggtctgc 420
gacggcgtgt acgactgcga agataaaagc gatgagtatg ctgaggagct ctgcaggcac 480
gtgtcccgtc cccacgccat tgtcgacggc tcgtactgcc aggagctgca cacgatggat 540
gaccggaact acaaatgtct ggacgcctcc ttctgcctgc caagcagcat gatgtgcgat 600
ggactgcagg actgccgcga tggcagcgat gagggacctt tttgcaagga ctggaacaca 660
atgtgcgaca acttcaaatg catgggtaac gacacgcgat gctccccgga gaggtacggg 720
cccacctgcc tgtgcctgcc gtcccacttc atgcgccagt acgactacat caccaagcaa 780
tgccaggacg tcaacgagtg cctgatggaa cgtcccccgt gctcccacaa atgtatcaac 840
gctgacggtc actatatttg cgaatgtgat cctgggtaca agagggatgt gtatggatac 900
ctttgttatg ctactggtcc cgaagcgatg ctgttcttta atactcggaa cgatatcaga 960
tacctgaaaa tcaagagtaa agaaatggtc acagtggcta ccgatatcat agagggtcac 1020
ggcgtatcat tcgatggcac ttacatttac tgggtggaga cggcacaggg acatcagtct 1080
atcttcaaag cgcagctcgg agacgtcaag gatacaaagg aggtgctggt aggtctcggt 1140
ctggaggacc ctggcgacat agcagtggac tacttaggcg ggaacatata cttcagtgat 1200
gcggagcgag ggaccatctc cgcctgcagg gtcgacggct ccatctgcac caccatcaag 1260
acctatgcta aaaacccacg attcgtcaca cttgatccta agaatggtaa gatgtactgg 1320
gcggactggc acgagcggcc ggtgataatg tcagctcgca tggacggtag ccaccacgac 1380
acgctggtgg acaccttgga gaacttcgcc accggcctgg cagtggacgc gcccaacggg 1440
aggctgtact tcgtcgacaa aactgtcaaa gtcgtcatga ttgcagagaa acatgtttat 1500
tctttatttg aagagccgtt ccaccacccg tactccatct ccgtgttcga gaacacggtg 1560
ttctggagcg actggacctc caacagcata cagacgactg acaaagtgca cgggaccgcg 1620
cagaagagga acgttctact caaacttgat acgcctgtac tagggatgca catgtaccac 1680
ccggtgttga tgaacacgac atcgaaccct tgcagcaaca acaactgctc gcacctctgc 1740
ttcgtcacct ccaacgccac gcacgtctgc gcctgccccg acggcatgga gatcgagaat 1800
aatcagtgtc atcatgtagg gaactaccgt gccaagtacc tggtggtggg aagtggacag 1860
ctgttcacca agatccagta caacgcgctc ggcaacccgg agtgccacgc cacgcacttc 1920
gacatcggac gcgtgcaggc catggcttat gatcggtaca gagattctct attcatatac 1980
gacggccagc ggcggaccat caactacatc aacatgagtg acttcacgct cggcgtcacg 2040
cacttgttga tctacaacgg gctggaaaat gtcgtcgaca tggattatga ttatgtgact 2100
gataacctgt acgttttgga cgcgagtcgt cgtgtggtgg aagctgtgtc tctgaggact 2160
cagaagcgcg ccatagtgca tcggttcgac attcaagaac tgcctattag cttctgtatt 2220
ctttccgact acgggaggat gttggtggct gtggtggaga gtgagatgca caataccata 2280
cacattgaca gcatcgggct cgacgggaac cagcggagac acgtcctcat gaacaatttg 2340
aaaggtcccc acattagatt gcggtacgtg gcagaaacgg aacaggtatt catatccgat 2400
gagagcaacg gcatcatcga cttcatacat cctgaaggaa ccgggcgaga gaactatcgg 2460
gagctcacct cgacggtgac cagcctcgcc atagcagaca actacgtctt ctggacagac 2520
cgaaagaccc ccaggctctt ctggtctgac atacacgagg cctcaccgaa gataaggagg 2580
atggatctgg cccttttccc aaacaccacg cagctcctaa tccaagccac aaactcgcta 2640
cctgatccga aagaccccct tctgaaccat ccttgtctga agaacccttg ttctgacgtc 2700
tgtgtccaac ttccccatga aactccacaa gatcacccga aactggcaaa cttcgagatg 2760
aagtacaagt gtttgtgccc acctggatta ctggttaacg gtaaccagtg cgccaaacct 2820
gcggcgtgtg gttcggatga gatactgtgc cacaggagca atatttgcgt gaagcaagat 2880
gctaggtgtg atggaaaggt ggactgtcct aagagtgaag acgaggaagg ttgtatagtg 2940
gatccggcaa acatatgtac atcagatgag atattttgtc gcggcttgtg cataaataag 3000
gagaaagcct ccatgtgttc tactggtgaa aaacctaata aagcgctacc ttcgaacaac 3060
tgcagcagca cagaattcca atgcacggac acgtctatct gcatctctcg gttgcaagtc 3120
tgcgaccaac acgtcgactg ccccaatgga tcggatgaac atctctccga gtgtgacacc 3180
tacgcttgtc acgaaactga gttcatgtgt gcgtccggct cgtgtatatt caagacgtgg 3240
acatgtgatg gagaccgcga ctgtaacgat ggatctgatg aaatcaactg cgtgaacatg 3300
acttgcggcc ctggtttcta ccagtgcaga gacagggagt gcgtagaact atcgaaaagg 3360
tgtgatggac gccgggactg ttcggattac tccgacgaag aagactgtga tgaggctcaa 3420
gttatcgaga aagtagaaga agccccaaaa tgtgctgcgt gggagtatac ttgtgaaaaa 3480
aatactagca tttgcttgcc tgaaactgct cggtgcaaca tgaagacaga ctgcccaggt 3540
ggcacggatg agcacggttg cgacctgcgc tgcgccccaa aaggaatgtt tgcgtgtggg 3600
caacaggtta cgtgtataac cttgaacaaa gtctgcaacg gccgtctgga ctgtgatgat 3660
gggtccgacg agacgcctga tgcttgttct cgggtgaaca ggacctccca cttattccca 3720
gtgtcccgta cattcagcga ctgtacggag ggttacaagt gcaacaacgg tcagtgcatt 3780
gaatggtcac aggtgtgtga caagaagagg gactgtgtcg acggtacaga tgaaaacgga 3840
ctctgtgata ctgcatgtgc aaacagcaca tgtaccttca tgtgtcagcc gaccccattc 3900
ggacggcgct gtctctgccc cttcggcttc caagtgtccc aggatcagtt ctcgtgcgag 3960
gacatcgacg agtgcactga agacgtctgc tcccaaggtt gtatcaacgt gccaggatca 4020
ttcctgtgct ggtgccatca tggttatgcc atcaggaggt cagaccgtcg ctcctgcaag 4080
gcgatccgcg gcaacatgtc cattctatac gtgtcgggta actccgtgcg gtctatctca 4140
gcggacgggt atgggtctat agagtacacc gatactgacg cctcggccat cactgatatg 4200
gattataatg ttaggcagaa gaagctatac gtggcttcgg aagagggcag caagttattg 4260
gaagtgaatg agacgcagaa tgtgatcgca gtcaccaacg ttgggaaacc atcgcgggtg 4320
gcagtggact gggtgacagg caacgtgtac ttcgtggaca cgactccgta cgaccagcgc 4380
atacgagtct gccacgtcaa gaggaagcgc tgcgcctccc tgctgaaact gccgtctgat 4440
gccacggtca cagcattgat agtggaacct agttctagcc gaatgttcta ctgcgtcact 4500
cggaagctgg agtcagtgat ctggacagct aacctggctg gcagacacgt gactgacctg 4560
gctactgtgc ggaactgcac cggactggcc gcagactctt tcaagaagaa gttgtatgtt 4620
gcagagacgg gcccagcaca tatcattagg atggattatg aaggggagaa tttcaacaag 4680
atcctctcag atcacccacg tcttcaagcg ccacatggac tagtaatctt cgaagactac 4740
atttactact tggaagccaa ctccttccgc ctcagccgct gccagctata tggagccaag 4800
cattgtgaaa catatgtgta tcgcgtgttt gacgccaaca catttgtcat tcgccacgag 4860
agcatccaac gtgatgacat cgtcaatgag tgtgaagatg tggtctgtga caatatttgc 4920
gccgtggacg aagatgggcc caaatgtctg tgtgatgatg gagctttggc gaagcgtggg 4980
aagtgtcccg aggttgataa gaaactggta ccattattca acggctggtc gtacgaggaa 5040
ctgaaatcag ctcacagcgt ctccttcacc atcatcgttg gagtgctgag cctaatcgct 5100
atataccttg gcgtgttcgt gtattaccac ttcgtatacc tacccaggaa gagaatgcta 5160
gctgccactt acactgaagt gcggttccag aacacaaata gttcgcccta tcccgaatca 5220
gacccaactg tggagatgca tccctcaagt tccgtttcac acgagttcat aaaccctctt 5280
cagttcgtaa gaaacatgtg gtatgggccc tttaggaaag atagacgacc aaattttagg 5340
aaagatagac gaccaaatgt aagtttaaac accacttggc attaa 5385
<210> 53
<211> 7020
<212> DNA
<213> 草地贪夜蛾
<400> 53
tctctacgtt ctcaatttcc gttccgttgc tcttccgccg tgccagcaac cacgtgtatc 60
aattaaaata ataattaatc aatttattaa tttaataaat caattttaat aattaaacaa 120
tcgttatttt ttttacttat taagtatcaa tgaaggaaat ataaataatg aagtatcaaa 180
gcttggtatt gatagttagt gtggcgtggt gctcggctca gctcacggat gacatgcaga 240
tgtttgagcc ggagtgcatg acggaggata agttcccctg catgggagga gggtgtatct 300
cggtgtctca gtactgtgac gggaacctgg actgtgaaga tggcagtgac gagaattttt 360
gcatcgaaca caagccgttc caagagttct gtaacgagac tcatcagtac atgtgtcagg 420
actccacgaa gtgtgttcct ctgtcctggc tgtgcaacaa cgagccggac tgtgatgacg 480
gcagcgatga gttcaactgc accgcgctgc cggctgtcaa tgttaattca acatgcaaag 540
gcttccaatg tggagacggc aagtgtatct ctttcctgtg ggtctgcgac ggcgtgtacg 600
actgcgaaga taaaagcgat gagtatgctg aggagctctg caggcacgtg tcccgtcccc 660
acgccattgt cgacggctcg tactgccagg agctgcacac gatggatgac cggaactaca 720
aatgtctgga cgcctccttc tgcctgccaa gcagcatgat gtgcgatgga ctgcaggact 780
gccgcgatgg cagcgatgag ggaccttttt gcaaggactg gaacacaatg tgcgacaact 840
tcaaatgcat gggtaacgac acgcgatgct ccccggagag gtacgggccc acctgcctgt 900
gcctgccgtc ccacttcatg cgccagtacg actacatcac caagcaatgc caggacgtca 960
acgagtgcct gatggaacgt cccccgtgct cccacaaatg tatcaacgct gacggtcact 1020
atatttgcga atgtgatcct gggtacaaga gggatgtgta tggatacctt tgttatgcta 1080
ctggtcccga agcgatgctg ttctttaata ctcggaacga tatcagatac ctgaaaatca 1140
agagtaaaga aatggtcaca gtggctaccg atatcataga gggtcacggc gtatcattcg 1200
atggcactta catttactgg gtggagacgg cacagggaca tcagtctatc ttcaaagcgc 1260
agctcggaga cgtcaaggat acaaaggagg tgctggtagg tctcggtctg gaggaccctg 1320
gcgacatagc agtggactac ttaggcggga acatatactt cagtgatgcg gagcgaggga 1380
ccatctccgc ctgcagggtc gacggctcca tctgcaccac catcaagacc tatgctaaaa 1440
acccacgatt cgtcacactt gatcctaaga atggtaagat gtactgggcg gactggcacg 1500
agcggccggt gataatgtca gctcgcatgg acggtagcca ccacgacacg ctggtggaca 1560
ccttggagaa cttcgccacc ggcctggcag tggacgcgcc caacgggagg ctgtacttcg 1620
tcgacaaaac tgtcaaagtc gtcatgattg cagagaaaca tgtttattct ttatttgaag 1680
agccgttcca ccacccgtac tccatctccg tgttcgagaa cacggtgttc tggagcgact 1740
ggacctccaa cagcatacag acgactgaca aagtgcacgg gaccgcgcag aagaggaacg 1800
ttctactcaa acttgatacg cctgtactag ggatgcacat gtaccacccg gtgttgatga 1860
acacgacatc gaacccttgc agcaacaaca actgctcgca cctctgcttc gtcacctcca 1920
acgccacgca cgtctgcgcc tgccccgacg gcatggagat cgagaataat cagtgtcatc 1980
atgtagggaa ctaccgtgcc aagtacctgg tggtgggaag tggacagctg ttcaccaaga 2040
tccagtacaa cgcgctcggc aacccggagt gccacgccac gcacttcgac atcggacgcg 2100
tgcaggccat ggcttatgat cggtacagag attctctatt catatacgac ggccagcggc 2160
ggaccatcaa ctacatcaac atgagtgact tcacgctcgg cgtcacgcac ttgttgatct 2220
acaacgggct ggaaaatgtc gtcgacatgg attatgatta tgtgactgat aacctgtacg 2280
ttttggacgc gagtcgtcgt gtggtggaag ctgtgtctct gaggactcag aagcgcgcca 2340
tagtgcatcg gttcgacatt caagaactgc ctattagctt ctgtattctt tccgactacg 2400
ggaggatgtt ggtggctgtg gtggagagtg agatgcacaa taccatacac attgacagca 2460
tcgggctcga cgggaaccag cggagacacg tcctcatgaa caatttgaaa ggtccccaca 2520
ttagattgcg gtacgtggca gaaacggaac aggtattcat atccgatgag agcaacggca 2580
tcatcgactt catacatcct gaaggaaccg ggcgagagaa ctatcgggag ctcacctcga 2640
cggtgaccag cctcgccata gcagacaact acgtcttctg gacagaccga aagaccccca 2700
ggctcttctg gtctgacata cacgaggcct caccgaagat aaggaggatg gatctggccc 2760
ttttcccaaa caccacgcag ctcctaatcc aagccacaaa ctcgctacct gatccgaaag 2820
acccccttct gaaccatcct tgtctgaaga acccttgttc tgacgtctgt gtccaacttc 2880
cccatgaaac tccacaagat cacccgaaac tggcaaactt cgagatgaag tacaagtgtt 2940
tgtgcccacc tggattactg gttaacggta accagtgcgc caaacctgcg gcgtgtggtt 3000
cggatgagat actgtgccac aggagcaata tttgcgtgaa gcaagatgct aggtgtgatg 3060
gaaaggtgga ctgtcctaag agtgaagacg aggaaggttg tatagtggat ccggcaaaca 3120
tatgtacatc agatgagata ttttgtcgcg gcttgtgcat aaataaggag aaagcctcca 3180
tgtgttctac tggtgaaaaa cctaataaag cgctaccttc gaacaactgc agcagcacag 3240
aattccaatg cacggacacg tctatctgca tctctcggtt gcaagtctgc gaccaacacg 3300
tcgactgccc caatggatcg gatgaacatc tctccgagtg tgacacctac gcttgtcacg 3360
aaactgagtt catgtgtgcg tccggctcgt gtatattcaa gacgtggaca tgtgatggag 3420
accgcgactg taacgatgga tctgatgaaa tcaactgcgt gaacatgact tgcggccctg 3480
gtttctacca gtgcagagac agggagtgcg tagaactatc gaaaaggtgt gatggacgcc 3540
gggactgttc ggattactcc gacgaagaag actgtgatga ggctcaagtt atcgagaaag 3600
tagaagaagc cccaaaatgt gctgcgtggg agtatacttg tgaaaaaaat actagcattt 3660
gcttgcctga aactgctcgg tgcaacatga agacagactg cccaggtggc acggatgagc 3720
acggttgcga cctgcgctgc gccccaaaag gaatgtttgc gtgtgggcaa caggttacgt 3780
gtataacctt gaacaaagtc tgcaacggcc gtctggactg tgatgatggg tccgacgaga 3840
cgcctgatgc ttgttctcgg gtgaacagga cctcccactt attcccagtg tcccgtacat 3900
tcagcgactg tacggagggt tacaagtgca acaacggtca gtgcattgaa tggtcacagg 3960
tgtgtgacaa gaagagggac tgtgtcgacg gtacagatga aaacggactc tgtgatactg 4020
catgtgcaaa cagcacatgt accttcatgt gtcagccgac cccattcgga cggcgctgtc 4080
tctgcccctt cggcttccaa gtgtcccagg atcagttctc gtgcgaggac atcgacgagt 4140
gcactgaaga cgtctgctcc caaggttgta tcaacgtgcc aggatcattc ctgtgctggt 4200
gccatcatgg ttatgccatc aggaggtcag accgtcgctc ctgcaaggcg atccgcggca 4260
acatgtccat tctatacgtg tcgggtaact ccgtgcggtc tatctcagcg gacgggtatg 4320
ggtctataga gtacaccgat actgacgcct cggccatcac tgatatggat tataatgtta 4380
ggcagaagaa gctatacgtg gcttcggaag agggcagcaa gttattggaa gtgaatgaga 4440
cgcagaatgt gatcgcagtc accaacgttg ggaaaccatc gcgggtggca gtggactggg 4500
tgacaggcaa cgtgtacttc gtggacacga ctccgtacga ccagcgcata cgagtctgcc 4560
acgtcaagag gaagcgctgc gcctccctgc tgaaactgcc gtctgatgcc acggtcacag 4620
cattgatagt ggaacctagt tctagccgaa tgttctactg cgtcactcgg aagctggagt 4680
cagtgatctg gacagctaac ctggctggca gacacgtgac tgacctggct actgtgcgga 4740
actgcaccgg actggccgca gactctttca agaagaagtt gtatgttgca gagacgggcc 4800
cagcacatat cattaggatg gattatgaag gggagaattt caacaagatc ctctcagatc 4860
acccacgtct tcaagcgcca catggactag taatcttcga agactacatt tactacttgg 4920
aagccaactc cttccgcctc agccgctgcc agctatatgg agccaagcat tgtgaaacat 4980
atgtgtatcg cgtgtttgac gccaacacat ttgtcattcg ccacgagagc atccaacgtg 5040
atgacatcgt caatgagtgt gaagatgtgg tctgtgacaa tatttgcgcc gtggacgaag 5100
atgggcccaa atgtctgtgt gatgatggag ctttggcgaa gcgtgggaag tgtcccgagg 5160
ttgataagaa actggtacca ttattcaacg gctggtcgta cgaggaactg aaatcagctc 5220
acagcgtctc cttcaccatc atcgttggag tgctgagcct aatcgctata taccttggcg 5280
tgttcgtgta ttaccacttc gtatacctac ccaggaagag aatgctagct gccacttaca 5340
ctgaagtgcg gttccagaac acaaatagtt cgccctatcc cgaatcagac ccaactgtgg 5400
agatgcatcc ctcaagttcc gtttcacacg agttcataaa ccctcttcag ttcgtaagaa 5460
acatgtggta tgggcccttt aggaaagata gacgaccaaa ttttaggaaa gatagacgac 5520
caaatgtaag tttaaacacc acttggcatt aaatgaccat aagtagacag ggtattcaag 5580
gtctattgaa aatttctagt actcattact tgtgtccaac cgatactgga tttttgccaa 5640
aaccgaaacc gaactttggg ccatggtttg gtcgtggtgg ttatataatc ttaatctaga 5700
aaaacaataa gcatcttcat aaaatcattc atgtatcttt aatcataaca aatcaaagca 5760
ttaaactgaa gcatacattt gtcttatatt ttttactttt agccgaaact ataccgaggc 5820
cgaaacttag gatccggttt cggccagaaa tccaatttca gtagacgcta cataataagt 5880
acttttgaaa ttttcttgtc acacaatcca ggcctatttg aatttaaata atcgttattt 5940
tgtataaaat aaatgtaatt tgttgcaggt catctccggt ctaccagtca ccacgcccgc 6000
atccccgccg caaccagatt tctccgacac agaatccgac ttagacgata aagagagtca 6060
gagaatttta aaatataatt aattttattc aatgagccta caaaataatt taatttttaa 6120
tctgtagcac gtttattttt attcacttga ttaatgatca agctaagaat tattttgtaa 6180
ttattatttt tattgcttta ttttttatgt tttaataatt taattattat gattgtgtgt 6240
aattgaatta tgtgttttat attatgttag tataaaatgt acaaagttta atttattgaa 6300
attaaaatta atgtaatttt tcatagtcca atcatttggt attggtgatt atacattttg 6360
ataatattat tcaccatcaa tagcctgtgt ggcagttcac tgctgaacat tgagggttag 6420
ccacgatctc ttttcgaatc tcatctttat tcattcatat cctggccagt tcggcaagaa 6480
ttcatattat ccacactacc ttaaaatgga taaacttttt aaactatctt tttcatacaa 6540
aatgactgga ctatgaatac tgaaataatt ataattgtat tgttatttat gaattattta 6600
ttcgatttgc agagttatag ataatacaaa tcaataaatt ggtgtgccat cgagtaagta 6660
attaatactt attccaataa aagtatcgcg tggaacagtt tttgttattt tcacattata 6720
aagacacacg gatgccttgc gacttactca cttatttaat ttatcacgaa aatagaaacg 6780
aacattttac actcacaata tgcaatacaa attgctattt ttacaaaaac atttacgtaa 6840
ctaaaaaaac gcgccaaatt atccataacg gaagtaggga gtctagtgtt gtccacacat 6900
atgactatca aactaaatac gatcactaat tatttatata tttgcaacca atagattttt 6960
tcaactattc aacgttgtgt taaatgtgaa taaattaatt taatttaatt agtcaaaaaa 7020
<210> 54
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 54
tgttaatttt gttctttgtg ctttcaacgt tattaaatgc 40
<210> 55
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 55
caggaatgct tcgcttatac atcttccatt tcgacaattg 40
<210> 56
<211> 39
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 56
ctttcaacgt tattaaatgc agtcgtttca acgaataat 39
<210> 57
<211> 39
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 57
atcacaatcc agttcatccg atccatctcc acaattgtc 39
<210> 58
<211> 39
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 58
tcaccaataa tatacactgg cccaatggtt tggctctag 39
<210> 59
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 59
tatgatgatt tttaccatta aacttatcac aagtttgaat 40
<210> 60
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 60
agaggataac gtcaacatcc acttagtgtc agtaacacgt 40
<210> 61
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 61
ttgccgatag ctcccatcca gctggacatg cacaaactat 40
<210> 62
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 62
aatattaatt ccactactat tagtattcct tggagcagta 40
<210> 63
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 63
ttgccgatag ctcccatcca gctggacatg cacaaactat 40
<210> 64
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 64
cctgtgtgat ggtgaaaatg attgtaataa ctttttggat 40
<210> 65
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 65
cgagtgaaga tctatgcaag ttttattgtt actacacaag 40
<210> 66
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 66
gtctgtgata ataagttaga ttgtcccgat tattctgatg 40
<210> 67
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 67
cttgatttaa atctttagcc actgtaaaat aatctaaaga 40
<210> 68
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 68
tagtgggaat agcatacgat ggacatcata tttactggac 40
<210> 69
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 69
gaacggtttg tcctgggatc catccataag tgaatatcca 40
<210> 70
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 70
cactcgtgga agaacataaa aatttaatct atggaatcag 40
<210> 71
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 71
caaatgtata aattattact ttttggatca aaatccattg 40
<210> 72
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 72
cgatacggag aggctagtcg tagaaataat tgatatgaat 40
<210> 73
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 73
ttaaaaccta tcgtaatttt cttctcataa gactcgctag 40
<210> 74
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 74
atggttttac atgtaataaa agggtaacct gcgacaataa 40
<210> 75
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 75
acaatgctca tctgaactat cagtacagtc atattctcca 40
<210> 76
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 76
tcttcagaag tttgtcagtc taactatttt aagtgtgaca 40
<210> 77
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 77
tggttattat tgcatactga ggtcatattt atgcatgcac 40
<210> 78
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 78
cgattgtttt gatggctctg atgaagatgg cctctgttct 40
<210> 79
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 79
ggaaattatt gtaacaatcg caagaatagc ctccttcttt 40
<210> 80
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 80
gaaagtatac acgaagtaag ctacaaagga ggaccggtta 40
<210> 81
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 81
tagcttacaa ctgactatat aaccagttgc aacagtatag 40
<210> 82
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 82
tatggagaca gatcctgcaa tcccagatat aaattacaca 40
<210> 83
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 83
tcttcggcca caattatcgg atttgtgtac tcgtgttgtc 40
<210> 84
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 84
tcttgtaaag ctcacggacc aagaatgacc atatacttag 40
<210> 85
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 85
ccatccaagt ttacactgta tattacgaag cttggtgttt 40
<210> 86
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 86
cgccatgcct gagggctaca tccaggagcg caccatcttc 40
<210> 87
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 87
ccaggatgtt gccatcctcc ttgaaatcgg tgccggtcag 40
<210> 88
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 88
atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa 40
<210> 89
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 89
ttaaaactgc ctggcacagc aattgcccgg ctttcttgta 40
<210> 90
<211> 20
<212> DNA
<213> 玉米根虫
<400> 90
aactctcgaa actgatggcc 20
<210> 91
<211> 23
<212> DNA
<213> 玉米根虫
<400> 91
caaacgatct tgttactgca tcc 23
<210> 92
<211> 147
<212> DNA
<213> 玉米根虫
<400> 92
aactctcgaa actgatggcc attgcgaaga tggtcattgg aagtgtgccg ataaattgtg 60
tattccactt gacatggtat gtaatggcgc acctgaatgt ttggatggat cagatgaaac 120
tattggatgc agtaacaaga tcgtttg 147
<210> 93
<211> 20
<212> DNA
<213> 玉米根虫
<400> 93
tgggaaatag ccacatcgac 20
<210> 94
<211> 22
<212> DNA
<213> 玉米根虫
<400> 94
cccagtactt agatcggcaa ag 22
<210> 95
<211> 140
<212> DNA
<213> 玉米根虫
<400> 95
tgggaaatag ccacatcgac cgttttttca tggatggtac tggaagaact cacgtcatcg 60
aaaacggttt agtgggccca gttcgtgtag tatacgaccg cagtctttat agaattttct 120
ttgccgatct aagtactggg 140
<210> 96
<211> 18
<212> DNA
<213> 玉米根虫
<400> 96
acacgtttgc cagtggtc 18
<210> 97
<211> 25
<212> DNA
<213> 玉米根虫
<400> 97
tgtgtccagt acaaacattt tctac 25
<210> 98
<211> 78
<212> DNA
<213> 玉米根虫
<400> 98
acacgtttgc cagtggtcaa tttgttattg agcaggaatc gcttcagcct aaagtagaaa 60
atgtttgtac tggacaca 78
<210> 99
<211> 23
<212> DNA
<213> 玉米根虫
<400> 99
ctaactctgg catcgaatac ctc 23
<210> 100
<211> 20
<212> DNA
<213> 玉米根虫
<400> 100
tgggcgtttc aaggtagatg 20
<210> 101
<211> 77
<212> DNA
<213> 玉米根虫
<400> 101
ctaactctgg catcgaatac ctccgcacat tcttacactt acctggagaa attgtcccat 60
ctaccttgaa acgccca 77
<210> 102
<211> 474
<212> DNA
<213> 玉米根虫
<400> 102
atgttgatgc caaaaaagaa tagagtatgt atttacgaat acctcttcaa agagggagtc 60
atggtagcta aaaaagatta ccatgcccca aaacacctcg aactagaaac tatccctaac 120
cttcaagtaa ttaaggcttt acaatcactt aaatcaaaag gttacgtaaa ggaacaattc 180
gcctggaggc attattattg gtatttgact aactctggca tcgaatacct ccgcacattc 240
ttacacttac ctggagaaat tgtcccatct accttgaaac gcccagcaag gacagaaacc 300
acccgtccta gaccagctgc tctcagatct gagacatcta aaccttcaga agaccgtgca 360
ggatacagaa ggactcctgg aggccctgga gctgacaaga aagctgatgt tggtccagga 420
actggagatg ttgagttcag gcaaggattc ggacgtggac gggcaccaca ataa 474
<210> 103
<211> 570
<212> DNA
<213> 玉米根虫
<400> 103
ggggctttct gatttttgac agcttctata gaagtttatc aagatgttga tgccaaaaaa 60
gaatagagta tgtatttacg aatacctctt caaagaggga gtcatggtag ctaaaaaaga 120
ttaccatgcc ccaaaacacc tcgaactaga aactatccct aaccttcaag taattaaggc 180
tttacaatca cttaaatcaa aaggttacgt aaaggaacaa ttcgcctgga ggcattatta 240
ttggtatttg actaactctg gcatcgaata cctccgcaca ttcttacact tacctggaga 300
aattgtccca tctaccttga aacgcccagc aaggacagaa accacccgtc ctagaccagc 360
tgctctcaga tctgagacat ctaaaccttc agaagaccgt gcaggataca gaaggactcc 420
tggaggccct ggagctgaca agaaagctga tgttggtcca ggaactggag atgttgagtt 480
caggcaagga ttcggacgtg gacgggcacc acaataaatt tattgataag ttaattttta 540
taaattgatc agccaataaa aagtttggtt 570
<210> 104
<211> 155
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 104
cgccatgcct gagggctaca tccaggagcg caccatcttc ttcgaggatg acggcaacta 60
caagtcgcgc gccgaggtga agttcgaggg cgataccctg gtgaatcgca tcgagctgac 120
cggcaccgat ttcaaggagg atggcaacat cctgg 155
<210> 105
<211> 155
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 105
atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca 60
ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa 120
gaaagccggg caattgctgt gccaggcagt tttaa 155
<210> 106
<211> 1770
<212> PRT
<213> 玉米根虫
<400> 106
Met Leu Ile Leu Phe Phe Val Leu Ser Thr Leu Leu Asn Ala Val Val
1 5 10 15
Ser Thr Asn Asn Asn Asp Cys Pro Pro His Gln Phe Arg Cys Thr Asn
20 25 30
Ser Lys Cys Ile Asp Phe Gln Gln Arg Cys Asp Gly Ser Asp Asn Cys
35 40 45
Gly Asp Gly Ser Asp Glu Leu Asp Cys Asp His Lys Cys His Glu His
50 55 60
Phe Phe Asn Cys Arg Asn Gly Arg Cys Ile Ser Glu Ala Phe Leu Cys
65 70 75 80
Asp Gly Glu Asn Asp Cys Asn Asn Phe Leu Asp Glu Lys Asp Cys Lys
85 90 95
Gly Gln Thr Val Leu Thr Leu Glu Thr Asp Gly His Cys Glu Asp Gly
100 105 110
His Trp Lys Cys Ala Asp Lys Leu Cys Ile Pro Leu Asp Met Val Cys
115 120 125
Asn Gly Ala Pro Glu Cys Leu Asp Gly Ser Asp Glu Thr Ile Gly Cys
130 135 140
Ser Asn Lys Ile Val Cys Asp Gly Phe Lys Cys Lys Asn Gly His Cys
145 150 155 160
Ile Pro Thr Glu Trp Ala Cys Asp Gly Leu Asp Asp Cys Gly Asp Lys
165 170 175
Thr Asp Glu Gln Asp Cys Ala Asn His Val Pro Leu Asp Gln Cys Thr
180 185 190
Leu Asp Lys Arg Lys Phe Leu Cys Ser Asn Asn Lys Thr Cys Ile Asp
195 200 205
Leu His Ser Val Cys Asp Asn Lys Leu Asp Cys Pro Asp Tyr Ser Asp
210 215 220
Glu Gly Lys Gln Cys Asn Ser Ser Ala Ile Ser Cys Asn Asn Asn Lys
225 230 235 240
Cys Ser His Thr Cys Ile Ser Leu Pro Thr Gly Pro Lys Cys Leu Cys
245 250 255
Pro Asn Gly Tyr His Thr Leu Asp Asp Ser Asn Cys Leu Asp Ile Asn
260 265 270
Glu Cys Thr Thr Tyr Gly Ile Cys Asp Gln Lys Cys Arg Asn Leu Pro
275 280 285
Gly Ser Tyr Glu Cys Tyr Cys Asp Asp Lys Tyr Thr Leu Gln Ala Asp
290 295 300
Lys Lys Thr Cys Lys Ala Thr Gly Gly Ser Gly Ile Met Ile Phe Ser
305 310 315 320
Ser Lys Gln Glu Ile Arg Ala Leu Thr Leu Asp Ser Leu Asp Tyr Phe
325 330 335
Thr Val Ala Lys Asp Leu Asn Gln Val Val Gly Ile Ala Tyr Asp Gly
340 345 350
His His Ile Tyr Trp Thr Asp Val Phe Thr Gly His Glu Thr Ile Ser
355 360 365
Arg Ser Ile Glu Asp Gly Ser Glu Arg Glu Val Leu Val Thr Ser Gly
370 375 380
Leu Ser Leu Pro Glu Asp Leu Ala Tyr Asp Trp Leu Thr Gly Asn Ile
385 390 395 400
Tyr Phe Thr Asp Ala Leu Lys Gln His Val Gly Val Cys Ser Asn Asp
405 410 415
Gly His His Cys Thr Val Leu Val Asn Lys Asp Ile Arg Lys Pro Arg
420 425 430
Gly Ile Val Val Asn Val Glu Ala Gly Asp Met Tyr Trp Ser Asp Trp
435 440 445
Gly Val Pro Ala Ala Ile Gly Tyr Ser Leu Met Asp Gly Ser Gln Asp
450 455 460
Lys Pro Phe Val Thr Asn Asn Ile His Trp Pro Asn Gly Leu Ala Leu
465 470 475 480
Asp Gln Pro Asn Ser Arg Leu Tyr Trp Thr Asp Ala Lys Lys Met Thr
485 490 495
Leu Glu Ser Ile Asn Leu Asp Gly Thr Asp Gln Arg Ile Val Leu Glu
500 505 510
Gly Ile Val Lys His Pro Tyr Ala Ile Ala Val Phe Glu Asn Lys Leu
515 520 525
Tyr Trp Ser Asp Trp Asp Ser His Thr Ile Gln Thr Cys Asp Lys Phe
530 535 540
Asn Gly Lys Asn His His Thr Leu Val Glu Glu His Lys Asn Leu Ile
545 550 555 560
Tyr Gly Ile Ser Ile Phe His Tyr Ala Leu Glu Lys Arg Leu Val Asn
565 570 575
Pro Cys Glu His Ala Ser Cys Ser Asp Ile Cys Leu Leu Lys Ala Gln
580 585 590
Ser Tyr Ala Cys Ala Cys Pro Glu Asn Lys Val Leu Asp Thr Asp Gly
595 600 605
His Thr Cys Lys Glu Val Gln Pro Thr Gln Ala Leu Val Gly Gly Thr
610 615 620
Gly His Val Leu Val Ser Ile Lys His Gln Phe Leu Gly Lys His Asp
625 630 635 640
Val Thr Phe Leu Pro Asp Leu Ala Lys His Val Gly Ser Leu Ala Phe
645 650 655
Asp Ser Tyr Lys Asn Ile Leu Tyr Val Ser Asp Leu Glu Thr Lys Ser
660 665 670
Ile Val Ala Leu Asn Met Asn Ile Gly Ile Ser Lys Thr Leu Glu Ile
675 680 685
Asp Gly Leu Gly Lys Val Thr Ser Met Asp Phe Asp Pro Lys Ser Asn
690 695 700
Asn Leu Tyr Ile Cys Asp Thr Glu Arg Leu Val Val Glu Ile Ile Asp
705 710 715 720
Met Asn Asn Leu Glu Arg Lys Ile Ile Val His Asp Thr Phe Gly Glu
725 730 735
Thr Pro Glu Ser Ile Ala Leu Val Pro Glu Asp Gly Ile Met Phe Val
740 745 750
Ser Phe Lys Gln Gly Lys Leu Gly Asn Ser His Ile Asp Arg Phe Phe
755 760 765
Met Asp Gly Thr Gly Arg Thr His Val Ile Glu Asn Gly Leu Val Gly
770 775 780
Pro Val Arg Val Val Tyr Asp Arg Ser Leu Tyr Arg Ile Phe Phe Ala
785 790 795 800
Asp Leu Ser Thr Gly Val Ile Glu Ser Thr Ser Ala Ala Gly Asp Asp
805 810 815
Arg His His Phe Arg Thr Leu Thr Thr His Pro Val Ser Ile Ala Val
820 825 830
Leu Lys Asp Asp Leu Leu Trp Thr Asn Leu Asp Ser Lys Asp Leu Tyr
835 840 845
Trp Ser Glu Lys Arg Ser Ser Glu Ser Tyr Glu Lys Lys Ile Thr Ile
850 855 860
Gly Phe Lys Glu Asp Asn Val Asn Ile His Leu Val Ser Val Thr Arg
865 870 875 880
Lys Gln Leu Glu Ile Asn Ser Cys Arg Val Asn Asn Asn Gly Cys Ser
885 890 895
His Leu Cys Leu Gln Ser His Lys Ser Ile Val Cys Ala Cys Pro Ala
900 905 910
Gly Trp Glu Leu Ser Ala Asn Gly Phe Thr Cys Asn Lys Arg Val Thr
915 920 925
Cys Asp Asn Lys Glu Met Leu Cys Pro His Ser Asn Thr Cys Val Leu
930 935 940
Lys Ser Leu Arg Cys Asn Gly Phe Lys Asp Cys Ala Phe Gly Glu Asp
945 950 955 960
Glu Trp Asp Cys Gln Thr Val Ser Gln Cys Leu Pro Gly Gln Tyr Lys
965 970 975
Cys Asp Asp Gly Gln Cys Ile Ser Glu Asp Leu Val Cys Asn His Ser
980 985 990
Tyr Asp Cys Lys Asp Lys Ser Asp Glu Tyr Gly Cys Ala Asp Lys Asn
995 1000 1005
Lys Lys Leu Gly Cys Pro Pro Gly His Phe Thr Cys Lys Ser Ser
1010 1015 1020
Glu Cys Ile Ser Glu Arg Phe Val Cys Asp Ala Phe His Asp Cys
1025 1030 1035
Asp Asp Gly Ser Asp Glu Leu Asn Cys Glu Asn Asn Ile Cys Leu
1040 1045 1050
Glu Ser Gln Phe Arg Cys Asp Val Gly Thr Cys Ile Pro Lys Asp
1055 1060 1065
Trp Glu Cys Asp Gly Glu Tyr Asp Cys Thr Asp Ser Ser Asp Glu
1070 1075 1080
His Cys Ser Ser Glu Val Cys Gln Ser Asn Tyr Phe Lys Cys Asp
1085 1090 1095
Asn Asn Arg Cys Ile Asp Pro Lys Leu Gln Cys Asp Gly Phe Asp
1100 1105 1110
Asp Cys Gly Asp His Ser Asp Glu Lys Phe Glu Lys Cys Leu His
1115 1120 1125
His Ser Lys Ala Pro Lys Cys Thr Leu Glu Glu Phe Ala Cys Ile
1130 1135 1140
Thr Asn Thr Ser Ile Cys Leu Pro Lys Ser Ala Lys Cys Asn Gly
1145 1150 1155
Thr Ser Glu Cys Pro Asn Asn Glu Asp Glu Lys Asp Cys Ser Lys
1160 1165 1170
Cys Glu Glu Asp Glu Phe Glu Cys Lys His Lys His Tyr Arg Glu
1175 1180 1185
Cys Ile Pro Arg Ser Trp Ile Cys Asp Gly Thr Asp Asp Cys Gly
1190 1195 1200
Asp Asn Ser Asp Glu Ser Met Glu Thr Cys Ser Ser Lys Leu Lys
1205 1210 1215
Pro Ala Ala Asp Asn Phe Ala Val Thr Asp Ser Cys Leu Thr Gly
1220 1225 1230
Tyr Arg Cys His Ser Gly Ala Cys Ile Asn Met Thr Ser Val Cys
1235 1240 1245
Asn Asn Asn His Asp Cys Phe Asp Gly Ser Asp Glu Asp Gly Leu
1250 1255 1260
Cys Ser Ser Ser Cys Val Gly Val Lys Asn Pro Cys Asn Gln Ile
1265 1270 1275
Cys Val Lys Thr Pro Ser Gly Pro Arg Cys Glu Cys Lys Pro Gly
1280 1285 1290
Tyr Lys Leu Leu Gly Asp Gly Lys Thr Cys Ile Asp Asp Asn Glu
1295 1300 1305
Cys Gln Thr Asp Pro Pro Ile Cys Ser Gln Leu Cys His Asn Lys
1310 1315 1320
Glu Gly Gly Tyr Ser Cys Asp Cys Tyr Asn Asn Phe Leu Leu Ser
1325 1330 1335
Ser Asn Lys Lys Ser Cys Lys Ala His Gly Pro Arg Met Thr Ile
1340 1345 1350
Tyr Leu Val Ile Tyr Gly Asn Gln Ile Arg His Leu Ile Pro Lys
1355 1360 1365
Ser Asn Thr Met Ala Val Leu Tyr Thr Asn Pro Met Ile Lys Ile
1370 1375 1380
Ser Ser Leu Asp Thr Leu Val Lys Pro Lys Leu Ile Phe Phe Ser
1385 1390 1395
Ser Tyr Glu Thr Gln Ala Ile Tyr Lys Leu Asp Thr Thr Thr Asn
1400 1405 1410
Met Met His Tyr Ile Arg Asn Val Gly Phe Pro Arg Thr Ile Ala
1415 1420 1425
Val Asp Trp Ser Thr Gln Asn Ile Tyr Tyr Phe Asp Thr Asp Val
1430 1435 1440
Asn Gly Arg Ser Ile Ser Val Cys Ser Phe Glu Glu Lys Cys Ala
1445 1450 1455
Lys Leu Ile Asn Ile Glu Ser Pro Arg His Val Thr Ala Leu Ala
1460 1465 1470
Val Asp Ser Val Asn Lys Leu Leu Phe Tyr Val Leu Arg Asn Trp
1475 1480 1485
Trp Val Leu Glu Thr Pro Ser Phe Val Ile Tyr Ser Val Asn Leu
1490 1495 1500
Asp Gly Ser Asn Arg Gln Glu Ile Val Lys Thr Thr Thr Gly Asn
1505 1510 1515
Val Glu Asp Ile Thr Phe Asp Ile Asn Lys Lys Leu Leu Tyr Tyr
1520 1525 1530
Thr Asn Lys Gln Asp Glu Ser Ile His Glu Val Ser Tyr Lys Gly
1535 1540 1545
Gly Pro Val Lys Thr Val Phe Ser Asn Ile Ser Gln Pro Glu Gly
1550 1555 1560
Leu Lys Phe Phe Glu Asn Gln Leu Tyr Tyr Thr Val Ala Thr Gly
1565 1570 1575
Tyr Ile Val Ser Cys Lys Leu Tyr Gly Asp Arg Ser Cys Asn Pro
1580 1585 1590
Arg Tyr Lys Leu His Thr Phe Ala Ser Gly Gln Phe Val Ile Glu
1595 1600 1605
Gln Glu Ser Leu Gln Pro Lys Val Glu Asn Val Cys Thr Gly His
1610 1615 1620
Lys Cys Pro Tyr Leu Cys Val Pro Ala Glu Ser Gly Tyr Arg Cys
1625 1630 1635
Leu Cys His Asn Gly Lys Val Ser Asn His Ser Glu Ile Cys Gly
1640 1645 1650
Glu Asn Asp Asp Asn Gln Ser Gly Asn Asn His Lys Phe Ala Val
1655 1660 1665
His Thr Thr Pro Ile Gln Thr Ala Ser Asp Lys His Ser Gly Ala
1670 1675 1680
Val Ala Ser Ala Ile Leu Ile Pro Leu Leu Leu Val Phe Leu Gly
1685 1690 1695
Ala Val Phe Tyr Tyr Leu Leu Lys Arg Arg Gly Asp Thr Gly Leu
1700 1705 1710
Asn Ile Ser Met Arg Phe Tyr Asn Pro Leu Tyr Gly Lys Pro Val
1715 1720 1725
Arg Glu Asp Gln Lys Gln Ile Leu Lys Pro Gly Gln His Glu Tyr
1730 1735 1740
Thr Asn Pro Ile Ile Val Ala Glu Glu His Glu Asp His Ala Lys
1745 1750 1755
Asp Ala Ser Arg Met Leu Asn Asp Ser Cys Val Cys
1760 1765 1770
<210> 107
<211> 5498
<212> DNA
<213> 玉米根虫
<400> 107
taccacagtc gacatctgac agtttctaca gtatagttgc agtgttcagt ggaaaatatt 60
caattaagga acctatatat tgttgactct gctacgcccg tcatgttggc acaactctcg 120
attatgttcc gaacagtgtt tgtgggattc tgaaaaacca gagcatcgtg aacgttcagg 180
acaatagtat tctctcttgg tgaatagaca gacttactga ctgtacgcaa tagcaccggt 240
gtaaaacttg atgacgttga tgatgatgaa gccatcgcaa gcaataacaa aaacaagcac 300
gaacgaaagg ttaattacaa tagcactgat agtatcgttt cttggtgatt catgtatgat 360
tgtgactaat cggtttgata gaaagaaaga tttattgtaa ctcctttcct catcgacctt 420
aaaaagtata tactacaatc aaaatcgttt tcaaagaact caaagggact ggcgagcttt 480
ggccgttgta gccgtgttct gttttatggc gttctatcgt ttataatgaa cagtttgcta 540
taaacgacat tacactagtt acaaatcagt tcgttgtaag tttatgatgc aaaatggaaa 600
gatgtatatt gcaggactat gtgctaatag aactgaagta cttcggaaaa taagcaagaa 660
ttagctcaga gtcataacag gtttccttac aagacaagtc aagttcccct atgggaaaag 720
aagtttgaaa ataacgaaac ctggtggtgg tcaaagaagt tcaataaaaa gaaaaagaga 780
agagaaaact atataaacag tggcaagaaa atagatcgca catagagagg aagcgaaagc 840
agcaaaagct aaagcagatg tgtgtacaac tctatacgat caacttgaca tcagttagaa 900
gtaagaagtt tacgtcagag agattgtaat ttttctatga agaaattgtt tttcgtatca 960
ttagtttagt ttgttcagtg aaagagaaaa tgaaaaaaca aggtggaatt ttaaggttat 1020
accgcacacc tggttgtaca gcagcaaaaa agtttgaaat tgtccagaaa cttcagcaaa 1080
catgtaaaaa tctaaaggat attcaaaccg aaatatgttt tcatatcgaa tcaacaagtg 1140
ccttggaagc aaaagacgtt aagattttga aatgggttct tcaagatcct ctccatcccc 1200
aaaatttaag cgaaaaggga acgctggtag cgaaaaatgg tacggctttg gttgaggtag 1260
gacctagatt taatttttcc acatcaagtt ccacaaatgc tgtttctatt tgccaaaatc 1320
tcggcttggt ccaagtaacc agattagagg tctcacgtcg ctatctttta gtgtttaatc 1380
aaggtacaaa acttacacct caaattgaga ataatctagc atctttactc tatgatagaa 1440
tgactgaatg caggtatact ccagaaaata tacctaaagt tagctttaat gaaaaacttg 1500
tcaaaaagga ggatataaaa gagattgata tattgggtaa aggtgcagaa gctttaaaag 1560
ccattgataa agaacttggt ctggcgttcg atgaagcaga tatagtatat tacacaaatt 1620
tgtttaaaaa tgttcttaag aggaatccca ctaatgttga atgctttgac ttggctcaat 1680
ctaacagtga acacagtaga cattggtttt ttaagggaaa aatggttatt gatggtgtag 1740
agcataagca atcactgata gatatgatta ttgaaactca agaacacaca aatcccaaca 1800
atgttataaa attcagtgat aatagtagtg caattaaggg attcttacac cgaagcttaa 1860
ggcctgtaac agcaggttct ctcagtgagc ttaagcaagt accagtagaa tctcatctta 1920
ttttcactgc agaaactcac aatttcccga ctggtgtagc tcctttcagt ggagccacta 1980
caggaactgg aggaagaatt agggatgtgc aaagtgtggg aaggggtggt tattgcatag 2040
ctggtactgc tggttattct gtaggcaatc ttcagattcc agattatgat ctaccatggg 2100
aagacaaaaa cttctcttat ccatcaaact ttgctccacc tttagaagtt ctagttgaag 2160
ccagtaacgg agcctcggat tatggtaaca agttcggtga acccttgatt tccggcttcg 2220
tacggtcctt tggtcttgtt gacgcggctg gagagagaaa agaatggatt aaacctatca 2280
tgtttagtgg aggtattggc agcatggaag ccgatatgac tgagaagctg ccacctaaat 2340
taggacacca aatcatcaaa attggaggac ctgtatacag aattggtgta ggtggaggat 2400
ctgctagttc agtcgaggta caaggggaca acaaagtaga gttagacttt aatgctgtac 2460
aaagaggtga tgccgaaatg gaaaacaaat tgaacagagt cgtacgtgct tgcttggaac 2520
taggttccaa aaaccctatc gtcagtattc atgaccaagg agctggaggc aatggaaatg 2580
ttttgaaaga attagtagaa ccagtaggag gaattatata cgccaataaa tttgatttag 2640
gtgatccgac aataaacgtt cttgagttgt ggggagccga atatcaagaa aataatgcgt 2700
tgttgtgtaa aaaagaagat cttcctcgtc ttaagagtat ttgtgcaaga gaaagatgcc 2760
ctgttaacgt tgtaggtgaa gtaactgata caggaagagt agttctggct ttagacgaaa 2820
gcaaggctgt tacaccgttt aatttggaat tggaacatgt attaggaaaa atgccacaga 2880
aagtcttcaa gttaaacagg cacaaagtag ccagtaaacc actagaattc ccaaaagccc 2940
tttcaatcta ccaatctctg ctgagagtat taaggttgcc atctattgga agcaagcggt 3000
acttaacaaa caaagtagat cggtgtgtta caggtttgat agctcagcag caatgtgtgg 3060
gaccactaca tacaccttta gctgatgttg ctgttacggc tctttctcat tttggacatg 3120
aaggtatagc gtcatctatt ggagagcaac ctatcaaagg tttagtgaac actgcagccg 3180
gtgctagaat gacggtagtc gaagctttaa gcaacctcgt attcgccggt atcaccagtt 3240
tacgcgacgt caaatgcagt ggtaactgga tgtgggctgc caagctccct ggcgaaggag 3300
cagctctgta tgacgcatgc aaagctatgt gtgacctcat gagcgagtta ggaatcgcca 3360
tcgatggtgg aaaggattct ttgagtatgg cagctagagt tggaaaagac actgtcaaag 3420
ctccaggaac cttagtagtt tctacttatg ctccttgtcc tgatattaga aaagttgtga 3480
cccctgattt caaagcgcct tctatgggaa aaacaggaac cattctgttc gtagatcttt 3540
cccgaagtga cagtagactt ggtggttcgg cattagctca agcatttggt caacttggag 3600
aggaatctcc cgatcttcat agcgctgaag aattagtaaa tgcatttaat gcaacacaac 3660
agttaataaa agatgattca attttggcag gacacgatat cagcgatggt ggcttaatcg 3720
tatgcctatt agaaatgtgc ttcgcaggaa tatgtggtat ggaagttgat gttcaacata 3780
gacaaggcaa accacttaat attttgtttg ctgaagaagt tggatgggta ctggaagttt 3840
tggatgaaga tgttgctcac tgcctgaatg tgttttcgaa tcacaacgtc ccagtattca 3900
aaataggaaa gactgttgga tatggagtta aatctaaatt tggaataata gccaaccatg 3960
cttgcctaga aagtacaatt ctacctttga tgaagctttg ggaagagact agttataggt 4020
tagaactaca tcagactatt aaatcctgtg ccgattcgga attccatagt cttccaagcc 4080
gaactggacc caaatacaca cttacattcg accctgatca cgatcagact gtaaaagacg 4140
aattagttcc cgtagctgtg ataagagaag aaggaacaaa cggagatagg gaaatggctg 4200
ctgcgttgat aagagccggg ttcaaagtgt gggatattac tatgcaagat ctgttgagcg 4260
gtgtggccac tttggatagg tttaggggcg tgatattccc tggaggtttc agttatgcag 4320
atgtcttggg ttctgctaaa ggctgggcgg ccagtattct attcaacaaa accgtccgag 4380
aacagttcga caaattcttt gccagaagag acacgttcag tttgggagta tgtaacggat 4440
gccaattgat ggctcttatc ggttgggttg gaacacctgt taatgaaggt actaaaccag 4500
acataatgtt ggaacataat ttgtctgaaa gattcgaatg ccggtggagc actatcaaga 4560
tagaaaaatc caaagctata atgatgaagg gtatggaagg gtcttcattt ggtgtgtggg 4620
tcgctcatgg agaaggtcga ttcacgttca aatcagacac catctacgac caattgaaat 4680
ccgaagaact cgtcgccttg cgttacgtag acgataacgg aaaaccgact gaaatctatc 4740
ccatgaatcc caatggaagt attgagggat tggcgggagt gtgctccgaa gacggaagac 4800
acttagcgat gatgccgcat cccgaaagat gcgttcaacc attccaatgg ccgtacatgc 4860
cacaaagctg ggtacactac aagaagagtc catgggagaa gatgtttaga aacgcttatg 4920
aatggtgcag aagcaataaa ttccataatg tttattatta agttaaaaga aatattgtaa 4980
atatttcaat gatctagctc aaattttata atgtttatac acattttgaa gttattctat 5040
tttagatata gcagtttcga atgtagagtt gagtccatgg atatttaccc gtgcgtcatc 5100
atttacagca tacgaaataa gtcggaaatt tacttaacgc aacagcaagt gacagaaagt 5160
ggctactgct ccgatgacgg atattataaa atttgacatt atcaaataaa tagaatgtaa 5220
aatgttagtt ttgctttaaa attttggtgc agaattaaca ctacatttga agtagcttaa 5280
taaattattt taatatcatt actgattaat aaatacttaa ataacaattt ttaaaaaatt 5340
taatgaaata tatatttttg ttatttcttt cacttttgcg gaaacttaaa taaaaccatt 5400
ccttaactgt gtgaatgagg ttaactttgt atgtaaatta atggcaaaat aaaatacact 5460
taccataaaa tttcaaactc caatataaaa ttagttgt 5498
<210> 108
<211> 1875
<212> DNA
<213> 玉米根虫
<400> 108
ctccgtttag cgagcggaat aacggcgtcg aatcaaacgg aaagacaaga gattgacctc 60
tctaaataat ccatctgctt gctatcagcc agcgagcaag ctacacacgg cccaagacga 120
aagaacgaag aaccttcgcc ttctctcctt cgccagttca gtatcccttc agcgacgctg 180
tgcatcgttg ctggtttaca acagtcgttg tagttcttcc caccttttca atcgctaact 240
caacggttga aaaccgacga tatatcctgc ctctgccttt gcctcatcga gtggggagaa 300
atacgggcag acaacgatct gtgtaatggc aatggcgttg tacagactat cagaactgaa 360
atcatggcct agttcccttt aaaagttcag aaaacagtga attcgcgtgg atagtgaaac 420
tggtgtaaat gagtgataaa aatttaataa ttctacgatg gctgaagcag aaaacatcga 480
tttccaagca tgttgcagac tgtgtctcag cgaaatggtg gattctttaa agtccatttt 540
cgacgaaact cctgaagatg aaagtctctc ccaaaagatt ttacaatcag tggctattga 600
ggtcagttcg gcagacaaat tatccaccaa gatctgcaaa gagtgcgtag aaaaagtaac 660
tgactggtct tcgtacaaag aacgatgtct ccaaaaccaa aagaaattaa cggagttgtt 720
agttgcgaaa gaaggtccag aagttttggt tgtacctgaa attatacccg aacccgaagc 780
accacttcct gtaaatggaa ttgaatcaga aactccttct gatgacgaca ttcatgaaaa 840
ccaagtggat cctgcctctt tgttggacct tagcttgcaa attaaagaag aaccagcaga 900
ccacactgaa cagaacgaac acgataaccc atatctagca tatttgggtt atcgtgttcg 960
ttctgttcag tgtggtctgc tggttcttct ttaatttgca agctaaggtc caacaaagag 1020
gcaggatcca cttggttttc atgaatgtcg tcatcagaag gagtttctga ttcaattcca 1080
tttacaggaa gtggtgcttc gggttcgggt ataatttcag gtacaaccaa aacttctgga 1140
ccttctttcg caactaacaa ctccgttaat ttcttttggt tttggagaca tcgttctttg 1200
tacgaagacc agtcagttac tttttctacg cactctttgc agatcttggt ggataatttg 1260
tctgccgaac tgacctcaat agccactgat tgtaaaatct tttgggagag actttcatct 1320
tcaggagttt cgtcgaaaat ggactttaaa gaatccacca tttcgctgag acacagtctg 1380
caacatgctt ggaaatcgat gttttctgct tcagccatcg tagaattatt aaatttttat 1440
cactcattta caccagtttc actatccacg cgaattcact gttttctgaa cttttaaagg 1500
gaactaggcc atgatttcag ttctgatagt ctgtacaacg ccattgccat tacacagatc 1560
gttgtctgcc cgtatttctc cccactcgat gaggcaaagg cagaggcagg atatatcgtc 1620
ggttttcaac cgttgagtta gcgattgaaa aggtgggaag aactacaacg actgttgtaa 1680
accagcaacg atgcacagcg tcgctgaagg gatactgaac tggcgaagga gagaaggcga 1740
aggttcttcg ttctttcgtc ttgggccgtg tgtagcttgc tcgctggctg atagcaagca 1800
gatggattat ttagagaggt caatctcttg tctttccgtt tgattcgacg ccgttattcc 1860
gctcgctaaa cggag 1875
<210> 109
<211> 3249
<212> DNA
<213> 玉米根虫
<400> 109
tgattttgat gaaaaaaaat ctgatgaaat agtagccatg aactctgacc tgggagaaaa 60
aatattattc gaaaaaactg taacatgcca gggaggtgtc gaaatttggc tgaaccatct 120
tcttaatgcc gtcagagaca ccgtcaaaaa cgtctgtgct atgcaggctc agtgttttaa 180
tgatcctgaa tacgacttca tatctggatt cgttcatttt tgtggacagg ctggactagt 240
tggtatacaa atattgtgga caaaagaatc cgaaattgct attaagaaag caagagtaga 300
tagaatggct atgaaaatta caaataaacg atttctcgac ctcttaaatg ctctcattga 360
tcttacatca aaagatttaa caaaaatgca aagaataaga tttgaaacca tggttactat 420
ccacgttcat caacgagaca ttttcgacaa catttacaag ttaaaagtta agtcattatt 480
ggattttgaa tgggcttgcc agacaaggtt ttactatgat gaagagacgg atgatataac 540
tgtgaaaatt acagacgtca tctttttata ccaaaacgaa tatttaggaa taacagagag 600
gctggcgatc acccctttaa ctgacagatg ttacataact ttggctcagg ctatttggat 660
taatatggga ggagcacccg ctggccctgc tggaacagga aagacagaaa cagtcaaaga 720
tatgggaaga actctgggaa aatttgtagt tgtattcaac tgctctgacc aaatggattt 780
tagaggactt ggtcgaattt tcaagggact tgctcaatca ggaacctggg gatgcttcga 840
cgaattcaac agaattgagc tacctgtgtt atcagtagct gctcaacaga tctacatagt 900
tttagaagca agaaaagcac gcaagccatc gtttatcttc atggatggag acaacgttag 960
tatgaatata gagtttggta tattcctcac gatgaatcct gggtacgcag gaagacaaga 1020
attgccagag aatctgaaaa ttatgttccg aagtgttgct atgatggtcc ctgatagaca 1080
gatcatcatc agagttaaac tagcttcctg cggctttaaa gaaaatgttc acttgtccaa 1140
gaagttcttt accttgtatc agttatgtga agaacaactt tctaaacagg tgcattatga 1200
ttttggcctt cgaaatattt tatcagtgtt gagaacactt ggagcacaaa agcgagcaaa 1260
tccaaatgaa actgaagaag tcgtagtaat gagagtttta agagatatga atttgagcaa 1320
actaattgac gaagatgaac ctcttttcct ttccctaata gaagatatgt tccctggtat 1380
taaactaact acaaaagctt ggaaagacct gcaaaaagct atcacgcata tatgccaaga 1440
gcttggtttc gtcaattatc cttcgtggaa tcttaaggtt gtgcaattgt atgaaacctc 1500
tttggtacgg cacggactaa tggttatggg tcccacgggt gcgggaaaaa caaaatgcat 1560
gttaactcta atgaagtcta tgacagaaat gggtatgcct catagagaac ttcgaatgaa 1620
tccgaaagca atcacggctc cccaaatgtt tggaagattg gatgttgcca cgaacgactg 1680
gacggatgga atcttctcaa ctttgtggag aagaactctt aaatttaaga aaacagacta 1740
ttgttggtta attttggatg gtcctgtaga tgcagtatgg atagaaaacc taaactcagt 1800
tttagacgat aacaaaactt taactctagc caatggcgac agaatagtga tggcaaataa 1860
ctgtaaattg gtattcgaac cggacaatgt tgataacgca agcccagcca ctgtatcccg 1920
aatgggtatg gtattcatga gttcttcggt ccttccttgg tcaccgatct tagaggcttg 1980
gttgaaaact agatcagcgc aagaagctga ttctatcaga aatcatttta acagatgcta 2040
tgatgacctt cacgttttcg tgcagaccag attaaaagcc aagatgtttg taagggaagc 2100
tctgtacgta agacagtgtt acgatgtttt acaaggggta ttagatatct ttgaagaacc 2160
aaaaacatgg agtgatagac aaatagaacg attggtacta ttctcaataa tgtggtcctt 2220
gggagcactg ttagagttag acgacagaca aaaacttgaa gaatttgctt taacccaccc 2280
gtccaaaatg gattggccaa aagctcagga aggagaaacc atttttgaat ttgtagttaa 2340
tccagacaat ggaagatggg aacactggaa atccagagtt gaaccctttg tatatcctac 2400
agataccgtg ctagaattta cttcgatatt ggtacctaat gttgacaacg ttagaacagc 2460
ctttttaata catacgatag ccaaacaggg taaagctgta ttgctaattg gtgaacccgg 2520
aactgccaaa accgttatga ttaagggtta ttgctcaact tttgatcctg aatataaact 2580
gaacaaatgc tttaattttt cttctgctac tactccaaac atggttcagc gtatcataga 2640
atcttatgtc gataagagag ttggtacaac ctacggacct cctgccggaa aaactttaac 2700
catatttata gacgacatta atatgcctgt aattaatgat tggggtgatc aaatcacaaa 2760
cgaaatcgta agacagtgca tggaaactgg aggattctat agtttggata aaccaggcga 2820
tttttcagta attgctgacg ttatgtttct gtctgctatg attcatcctg gaggtggtag 2880
aaatgatatt ccccaccgtc tcaaaaggca gtttagtata ttcaactgta cactgccaag 2940
tacttctgct atggatgtta tattcagtca aattgcttgc ggctactttt gtctagaaag 3000
gtttaacgaa gaaatggcag agtttattcc aaagctagta ccacttacgc gacatgtatg 3060
gcagaatacg aagaaaaaaa tgttacccac tccagccaaa ttccattatg tatttaatct 3120
acgagatctt tctagaatat ggcaaggaat attgacagtc caagtgcttg agtgtccgac 3180
taagaatatg gctcttaagc tctggagaca cgagtgtact agagtaattt cagataggtt 3240
tactgaagc 3249
<210> 110
<211> 1370
<212> DNA
<213> 玉米根虫
<400> 110
cttaaacata accttcaatt tgtaatgtgg ttttaaaatt cattccttaa ctttaaattt 60
atttattttc aattaggaaa attaggctat aacgtcgaaa aatgtccaaa aaacgctata 120
attggaaagc taggcaaaat gttgaaactc tggttgataa tactcataaa gaaaaggaaa 180
tggctcctcc accatacgca gacctcggca aaagagcaag agacgtattc ggcaacggtt 240
atcactttgg cctaattaaa ttaaattgca aaaccaaaac agccagtggg gtccaattta 300
gtacaggtgg tagttcagac catgaatctg gaaaagtgtc tggatctttg gagagcaaat 360
acaccgtcaa agattatgga ttgactttca cagaaaaatg gaatacaaat aatactttgg 420
ggactgagat cgccatttca gaccaaggta ttaagggact caaggtctct ggtaatgtta 480
acttctcccc acaagaagga taccagtcag gagttgtgaa gtctgaatac ggcaacgaaa 540
gaatacacct caacgccgat gttgacttaa atagtaacgg acctgtcgta agaggttcgg 600
ctgtcgtggg ctatcaagga tggttggctg gttaccaggc tgcctttgac gttcaaaact 660
caaaattggc caagagcaat tttgcattag gattttcaac atcagacttt gttctccata 720
catgtgttaa cgacggccaa acgtttggcg gttccatcta ccaaaaaatc aaccccaaat 780
tggaaacagc catcaacttg tcttgggcag ctagcggaga aaagaccgac ttcggtttgg 840
gctgcaagta tgacttggac gccgacgcat ctcttcgcgc taagattaac aacgccagtc 900
aaatcggttt aggataccaa caaaaactac gtgatggtgt aactctaact ttgtctaccc 960
tcatcgacgg caagaacttc aatcaaggtg gtcacaagat tggtttggcc ttggacctgg 1020
aggcttaaat atgttattac atcttgttcc acaaatacat attagcgccc atttaatata 1080
tttaagaatc ctgtattgta gataataatt atttaaacgg gactttagtg ttatacttta 1140
ttactcccgt tgtgtttatt tcagttgctc ctcccaatta ctcctttttt acacgctata 1200
cgtatatatt gtttttatta ttaaactctg aaaggcgcgc ttttctggat attttcaaag 1260
attgatgtca agttaggagc atatgcattt tatgtcaaaa aattagattg ggattgtggg 1320
tcttagatgg ctacaatccg ctttgtgtag ttttgcaatt ttgcaaaaaa 1370
<210> 111
<211> 1194
<212> DNA
<213> 玉米根虫
<400> 111
acccattggg gaacgcacgc attttgttta gttccggcgt ctttgctcaa gttttgcccg 60
gttgttgctg tgtttggttt gttggtgcat tttattatta taacaagttc attacaatgg 120
gcaaaaagaa ggtagaagag agtgaaagtg aagaggagta ttctgtagag aaaattattg 180
ataggagagt tgtagatgga aaagtagaat attatctaaa atggaaagga tattcagaag 240
atgataacac ttgggaacct gaggacaatc tagattgtcc tgaacttatt tctgagtttg 300
agaggaatcg taaaagtaag aaggctgctg gcaaagaaaa gaaaagaaca agagataatt 360
ctacatcttc cgtggattca aacaattcat ccactaaaga aaaagaaaaa aagaaaaaga 420
aaggccgttc tccatcaccc gagtctgaaa gtgaacccag ctctaaaaaa tcaaaaatag 480
atgattcaga tgatgacaaa aagagcaaaa agaagtcgtc atcaaaagat gctgattccg 540
atgatgataa aggaaaaaag tcaaagaaga aagacaaaag tgctttagat gatgactctg 600
acgatgagaa gtcaaaaaag tcaaaaaaaa agattctgat tcagaagatg aaaaaaagaa 660
aaagaagaga aaaagtggtc ctaaatctcg taaagctgcc gtttcagatg actcagatga 720
agatgaagcc ccaaaaaaga aaactgaaag tcgggcgaaa aaagatgatg aaaaatccaa 780
gaaaaaagct gatgattctg atgatgaaga tgatccgaag aagagcaaaa aagttgacaa 840
caaggaaaaa aagggtgcta aacctaaaga caaagagaag tctgcatttg acaaaggttt 900
agaagctgat aaaattattg gagcatctga cactagtggt caattgatgt tcttgatgaa 960
atggaagggt acagatgata ctgatttagt ttatgcaaga caagccaatt tgaaatgccc 1020
acaggtggtc attaaatttt atgaagaaag aattgcgtgg catacgccag acgactttta 1080
attttactta gtgataagat ttgttgtaga tttaatttct tacttttaaa taggtagagt 1140
tcttatatta ttaatccata tgtatttttt ttaaatggaa gaaaccacga cttg 1194
<210> 112
<211> 1802
<212> DNA
<213> 玉米根虫
<400> 112
gggggagaaa acatatgatt gcccagactg acagtaattc gatattttga attaattgaa 60
ctgaacactt ctattattaa aactaatcaa aacactcgaa acagatacgt aatgcagtta 120
taatgagttt tataattatt gcttttaaaa atttcctaca taccatgttg cagttattat 180
cttttggtaa aaaaactatt acaaatagtt tgagtgtgta tgtaaaaact attggtggca 240
acactttaac tgtagatcta gatcctacat gggacattaa aaatgttaag gaaatagtgg 300
caccacaact tggattagaa ccagatgaag taaagatcat ttttgctgga aaggaattgg 360
gagacgaaat taaaattgag gaatgtgatt taggtcagaa aagtattctc catgctgtaa 420
aaattagagg aaagaggagt aagcttattc aacctacaaa tagtagtgta atagaggaaa 480
atgaagatcc aagtagcaag ccattgagtg aggttttaga tgatagtatg ttctctcatg 540
aagacagcaa ttcttttaat agtacaactg aaaggcggtc atctcaatca gaaagtggtt 600
taattaagtc aaaagtacat ttctatgtat tctgtccaac atgtaaggca atgaagtcag 660
gaaaaataag agtaaggtgc catttttgta aaagtggagc gatcactgta catgctgatc 720
cccaaaactg gaatgatgtt ctaatatcaa aacaaattac aggaacatgt gaaaatagtc 780
cagaattatg ttctaaccta ttaaaaaacg tagagcctac ttttgctgag ttttacttca 840
aatgttctga acatacatca ttaggggaac aagatggagc agtgccacta gatctaataa 900
ggccaaattt aagggacatt ccctgtttag cctgtgcgga tgttagcgat cctgttcttg 960
tctttccttg tcaagaaaaa catgttacat gcctggattg ctttcgccaa tattgtgtga 1020
ccagattaat ggaaagacag ttctggcaac atccagagct gggatacacg ttggcttgtc 1080
cagctggttg cctagactcg tttataaaag aagtccatca ttttagattg ctcacagatg 1140
ctcaatatgc gcaatatcaa cgtttcggaa ccgaagaatt cgtcctcaga tccggcggag 1200
tactgtgccc tcaaccgggt tgtggaatgg ggattttagt tgattccagt tgtaacaagg 1260
tggcgtgtat aaacggttgt ggttacgtat tttgtcggtt atgtttacaa ggctatcata 1320
tcggcgagtg tcgaccatcc gatatggaca gttctgcagg tggtgaagga tgtttgtaca 1380
gcgtagaccc aaatcgagca tcggacgcaa ggtgggacga agcttcgaga gttaccatta 1440
aagtttccac gaaaccttgt cctaaatgta ggacacctac tgaaagagat ggcggctgta 1500
tgcacatggt atgtactaga gcagggtgtg agttccattg gtgttgggtg tgccaaactg 1560
catggaccag agattgtatg ggaagccatt ggtttggtta agacattgaa aaatatgtga 1620
tattaataaa ctaaaaaatg tgtgttcttt tgattgtaca gttgtgtttc acttaacaaa 1680
ggttgtttgg ttattttttg ctatgtttga atcaacagac cttaaaatgc cgttctgtgt 1740
catacaaaag agaccacaga gtacagaaga agaatcatca gatctattat agatatagaa 1800
ta 1802
<210> 113
<211> 15730
<212> DNA
<213> 玉米根虫
<400> 113
agcaaatggc atggcagatt acaatggcag cgagcagtgg cggagtagat tggacgactg 60
tcttaaaacc attcttatct aaaaattatg agacttctaa tacagcagaa ctgatagaac 120
tatgttcagc tattgtaaaa agcgaatccg aaatcttgcg acacaaagag agtaataaga 180
atttcttcaa ttcctttgcg gttctagctt cagattatat tagcagtagt actagtggtt 240
tacttgcgag tcaactagaa acggtaaacg ctgcatgtcg tatactatta aagtatctgc 300
ttagtgctct cagtagcgca tgtagcgaaa ccaatccgag cagtgcccaa atcgaacgat 360
atttaaatgc tattaaagta ctttgcatag gtactggact gctgtccacc acggaggtca 420
ccgtcctcgt cgacagtatg aagggagaaa atttacccca acagaaccca gtgccttcag 480
gaaatgaaaa agatgtcagt aacaaacagg aatcgtctaa aaatcgatcg gatttatcag 540
tcagcattta cgaacaatta acactaccat taagagatgg ccgctcagta tctctcgata 600
ctggcgctag cggttcctca actgtacccg aagtgacacc tggtagcgat ccttcatcag 660
aaattaatcg tttgttttta aaggcaaaca cagaaagttt acaatcgtta agggccggtg 720
acactttgat agatctgtgt cttagtttac catccatcaa aaaagccaaa ggcaaagttg 780
aagatgcttt ggccggaaaa cctttttgta tacctaccaa tcatgcagaa gcgacaactc 840
ttagaaatac actatcgtca accctatctg acattaacct agccatgtcg gctataaacc 900
taccggtctt agaaccgtta acctcgagca aactagacaa attgtgtagc ctagctatgg 960
cagttctaca ctgcgccctt agtcaagcct cagcagctgc ggttctttct atgggctcag 1020
tggtttcacc taagtgctca aatcaacagt cacaagctgg tttgaaagaa gacgatttag 1080
attcaaacgc agtggcttta gtcgaagaga ctctaaatat gtataatttt attggaaatg 1140
ttattaaaac ttctacgagg gcaggaggtc atgtgtatca aaattattta cttgctgggg 1200
catgggtgct catttcgggt ctccaaacgc atctcacctc cagtacgtct gcggaaaaat 1260
caccacatct tagagaaagg gaggaaaaag gtagaagccc ttgtaaaagt cgagacggaa 1320
atactgccag atctggatta caaaaatttc agcagtcttt tggagtactt tcggtagctc 1380
tggcttctag ggctcttagt ttgttatccg aattgtttga cgatttatat ttagaagtgt 1440
gtggtggagc agggagcata gtccaggttg aacctgctcc cttagcaata atgggccagt 1500
tcacagcatt gcagagaatt tctaggatat tgaatgcggc tcccttaaat caccttttgt 1560
tttatctagc aatagtcagt tatagaaaag catgtacact taaaagatta catccgccag 1620
agggcgacac ctttagccaa tcagattcaa cgacatatta tgaagatatg atcatgtgct 1680
ccgacgatag ttcaacggat gaagacgatg atagtgagcc catcttagga caatggtttg 1740
aagaaaccct tgcgccccca gaaacaaccg agtccaaatc accaacttct gaaaatggag 1800
aaactaaaac tgtccaaaac gaacgatctc gatcgatggt cccggaaaaa ggagaagctc 1860
acggatttat cgctctttca actagtgttt ttgagttctt aaataaacac tttctgtgta 1920
cgaaaagtac ttttatatcg aggtacgtca aaaatggctt aacggaacag caaatgatta 1980
ttttggctgc gatcattaga gacttggaca gggagacagc gagaactgaa ataggaacaa 2040
tttcagtgta tttcggccaa gcactaggtc agctttattg cgaattctcc ggagcactaa 2100
ctagattcgc acacaactta gtaacaaacg gaaatttgag taatttacaa gcttgccttt 2160
taaatcatct aggagtatct ccctggaata ccgatgtccc tcacgcgtgg cccttacaag 2220
tatatccaag gacattggct gtcttagctc aggttttgct tttgagacca cagaacgaga 2280
aagaggcatc tgtaattagt atatggcata ggttggtgaa tacactcata gaaaatgttc 2340
ttaacaatcc tcattctgtc gttgaatcag aaaatgaaga tttaaatgtg gaacatgccc 2400
aagtgctcct ctatttattt cattcattga acttgatgca gaagaaatca gtcgtgttac 2460
ttatggctgg tggtgtttta agatgttccg agatagcaag aggtccactt aaagattcac 2520
agttgctaca cttatctaga gtattgctat tattcgatta tattatgaag cacctatacg 2580
atccacctac gtcgttgtta gaacagattc aatggaacct cttctacttg acaaacctga 2640
attccgacaa agaaaaagaa aatacaatga cccgtatgta caccgcttgg caggacatag 2700
aagacaacta caggaaaata tcggcagttg atgaattcgc catgaaacct cggttctacg 2760
ttttgacaaa tttggaagtg aacaatcaag atgctccgaa actggacggt ttggcgtgta 2820
attttatctt gggaacccca gataaactac gatatccttt gttgttggat gctctaattg 2880
aaattttgaa cgtaacacac atcacttcag gtgctaacgc gtcgaaaatg agtttcctgg 2940
gactttgtgc tacacaatac tgtttcacta tttgctggag gctattacag ctactcccgc 3000
catcagcgtc atatatggaa cgcttggcga caggcgaaaa tttgacggct ggccccttat 3060
tacttcattc tctagtctgg gggcctagag cgtcccacaa gaatttcagt agatggctga 3120
aggactgtct agttaagcaa gggatgtaca cgcaacacac agacaaatta ttaaaggaag 3180
tgtccgaagc agttaacaat attaaataca atataacgaa tgctaaaaat tgtattatgt 3240
cgcttactcc tgatgtcaaa aaaggaatgg taaccaaaga aaaccttcca ccattatggc 3300
atttattttt actcgacgct cttatgacaa acgtacaagt ggctttagaa gagcccgaag 3360
ggagcactga aagtacttca atcactgtat ctaacggaac ctacgtccag gatttattac 3420
cccacgtttt aaggttaact caagcaatac tgcactgtac aagatggtcc ttactacata 3480
ctattgctga ccaaaatccg tcgtgtgaaa aattctcatt gcagaacttt gaaggactac 3540
aagatgtcct tgcaatagct tctacaaaga atacactaac gatgtcgtta tcttctgagc 3600
taaccactct tttgccttca tgtgtaagct ctgcccagtt gcagaattat aagacaattt 3660
tagaagatgt ttcattgagt gcttatcaaa acgacataat cccatcagaa agcgccattt 3720
taaaagtagt agacgcacat gtagctactc tttccatggg cagcccatac agcatcaact 3780
tgtcacttcg acgtttatta caatgcctag tcaaatttat ttgtgatcat gcccctaaaa 3840
cagaaaatac agacacgaag aacaaagcga ttgaactact agtttcaatc actttagatc 3900
taaggaccga atttttgaat gacactgtga ctaaaacgtt agacaaaatg attggcgata 3960
ccgaaataga cgaacatcaa aaacgagtct acctgagggt tttagatcat acttataaac 4020
tcataataaa ttacacgtca ggtaactcag agacgtatag tgctaatatt gatgaaaaaa 4080
tacttcacca ctgtttgaaa ttctacgaaa aaatcatcga gaaatcttca ggcagacaag 4140
ctttagaaat cttctttact ggagataggg atttggtgaa agtgctgatg tctgtatcta 4200
gtccgcaaat gtcgcaacat tatggtacca gagtactgca tttcttcaat aaactgttcc 4260
aagcagccga gaagagctct acagatccaa gcctgaacta cttatgttca agtatgagta 4320
aactggcaaa cgtagatagc gaaaagctgc aaacctggtt gcgtcaaatc atcatcggta 4380
caagtagtat tgaacccgtc gtgacaacta cagttaccat caataaagaa gaatctcctg 4440
cgacatctac caacggaaaa tggactataa ccacggttga aagtaaagag gctaattctc 4500
caacatctaa cgacgatcaa aagtccttgg tgcaagagaa cagtcagctg ttgcaagctt 4560
taactagttt tattgttaaa caaaacagta atgtttccga agaagtttcg ataacaatat 4620
tgaaggcgct gatccaactt ggaaaacatc tgttatcgcc tactctggaa ggtactggtt 4680
ttactgattt aatggtggat atgatcatgt tagcagatgc tggttcagga aagggacaca 4740
gttatttatt ccctgctaca gcagaatgga tagaactgtg caacaaacgc atctcagaaa 4800
aagatgttat agataaatta actagtaatc ctgattcctt gaagaaaaat gctatgctgg 4860
aagcctcttc tagtattcta gactacgtta gcgatgttat atcaactatt attgcccagc 4920
atcctcaaac gataagacct cttagtcctc cttgggaaag tgagtctcct ttagagttgg 4980
aatctgagtg gcaagacttc gcgaacgatg acgatgatag cggtgaagat agcgacgagg 5040
attctttatg caacaaactt tgcaccttca ccgtaaccca aaaagaattc atgaatcagc 5100
attggtatca ctgtcacact tgcaaaatgt tagatggcgt tggtgtatgc tctatctgtg 5160
ccagagtttg tcacaaaggg cacgatttga gttacgccaa gtatggtaac tttttctgtg 5220
attgtggcgc caaagaggat ggaagttgtc aagcgttggt aaagagaagt ccccaaacca 5280
acgaaaccaa taatgcaaca actggaaaca actctgaaca tatgttaacc agttctttaa 5340
gaagaaagac ttcaagtccc atcccagctg acaagattat ttataaggag cgaaaaaatt 5400
cgaacactgt caaacagtta gatggttcaa aagaattcat catcaactac ttaggatcaa 5460
gtttggtggc aggatctctt ttagaattcc tacaatcttt aatcccagct attgaagaaa 5520
actgtcgtag aaattcactt gtaggatgcc acttacgcgg tattaaggcc ttggatcaac 5580
tatactcaaa cgaaaagaaa tatgtacata ctgatcaact tatggtacca actttaggtt 5640
ctcaagaagg agcttttgaa aatgttagaa tgagttatgc tggtgaacaa ggacagacca 5700
taagacagtt gctttcggcg catatagtac gaaggattgc gatgtgttgt atgacgtcta 5760
cccaaggtcg tagacagcac ttagcagtat cacatgaaaa gggcaaaata accgtattac 5820
aactgtcagc tttacttaaa caagctgatt cgtctactag aaagttgaca ttaacaagac 5880
tatctacagc accaatacct tttactgtac tatcgctatc aagtaatctt tgtaacgaag 5940
actttttggc agtctgtgga ttgaaggatt gccatgtact cacattcacc tctgggggat 6000
cagtttgtga tcatttagtc ttgcatcccc aattagaaac tggaaatttc atcataaaag 6060
ctatatggct gcctggttct caaaccaaat tagctctcgt aacagcagac tttgtcaaaa 6120
tctacgactt agccacggat gcattaagtc ctcagtacta ctttttggta ccgagtggaa 6180
agatacggga ttgtacgttc atgtacgatg aaggcactta tcacatttta ttaatgtctt 6240
caccgggaca catatacacc gaaattttaa atgatgattc ctctacgaag tatggttcat 6300
tttatgtgac gaatacctta gaagtttttc atttagaagc agcggatgtc attgggcaag 6360
tagccggagg tggagtatcc atctattact cacatactct tggtctccta ttctatagct 6420
acgcccaagg aaaaagtttc atatctccca tcaatcccaa aagcaacagt ctaccgttag 6480
tatttgccat aaacctaccg caaaccaata acaccacttc aaaaagcaat ggttcgaaaa 6540
attctgccaa ccaaccgtta tgccaatgga ccgagatccc aaaccatccc ggtttgattt 6600
gttgcgccat gcaatcgaat aacaatcctg ttattttgat gcttaagcca gacactattt 6660
tgatacagga gattagagtc ttaccagcca aaagtaagat tatggatatg gttgccatta 6720
gacacagttc cggaaatgag ttgagaacaa ctttgattct gctgtgcgaa gatggtagct 6780
taaaaatgta tatggctaat atggatcaaa caggtttttg gatgtcgtca agcattcaag 6840
ctaacattac caactcgtcc gtaaaaccga aaaagaagaa aataataaaa tcggggaaga 6900
ctacaaattc cgtaaacttc ccagtggact tctttgaaca ttgcacaatt ttgaccgaga 6960
ttgaactggg cggtaatgac gttttgcaaa tttataacca ggcacaactg aaaaatcgac 7020
tgaacactac tggtttatac atagcttgta ataaaccact aggtttttct ctcgatgtaa 7080
ctaataatga tccgaacatt gttatggtag gactgagagt attagttgga agtcaagatg 7140
tcataaggat cccatctttt gtagagatat ttggacgtac aattcctata acagcaactc 7200
gcagccgctg gtatgacatt gcattctcaa gagaagaagc tcttcaatca gacaagaaag 7260
tcacaattct atttggtcct tctcaagatc ctgaaactgt tactatggtc gactgtgtaa 7320
aaatttatgg aaaaactaag gattcatttg gatggcctga agaaagtgac gaaaatgtag 7380
ctgctggtgg tacgggaagt tcccaacagg ctgcttccaa ttcagatagt gatcagagtt 7440
caggaaataa aactcaatta acgaaacttg aaaagcttgt ggttggcata cttgaatcac 7500
tcgatggcag cttctctctg cattcaactg aagacaaact ggctaccttc aagacaccaa 7560
cacttaaagt tgcaacgtcc ttgttgaccc ttcccaccca gccgtcagta caaatccatt 7620
ctaaggctct tttatcatct cttcactcta caaggctact gtatcataat tacaaagacc 7680
aagcgttact tcaacatgtt ttgacaactt tgacggaaat gacaagtacc aacattaaag 7740
atttagatgc cgagagctat tatagactgg ttttgattgt aagaggaatc gctgttgcta 7800
gaccacagaa tttggtgaaa tttgctgata gccatacttc gatacaagat gttgtcctgg 7860
aagatcctct ggaaaatatt ataagagaaa agcaaggttc aacgtccaaa gaagtcaaac 7920
catttgaacg taaacaacat agtcagcatc ttcttcttca gctaatggag gttctttggc 7980
tattgcactc tctgaatcct gaaattcctt cattgtccct agtagtttca caaggactca 8040
agcacaccga acaaattgtt catgcacttg ttgaaatagt gcacgccttt aatagctgtg 8100
atacatacag caatgtcact atagcagtgt atcttcaact gttattgtgc aatgatcctc 8160
tgattgcatt tagtgctaaa caagctctta gcatagtgct taaaccaaag acgaagagaa 8220
gaaaggtttt catacctagt cctcctcatt gcgtttcacc gcctcttcag aagaccacag 8280
atgataggtc taagactcct cccgctatac aacaatcggt tagtcatgaa gaagagatgc 8340
aagcaagaca cccaaatcaa tatgatgtgg acgccattga agctattgga ttactagaac 8400
agcctggtca acagcaagac aaccacaatg ttaatgcttt ggaagcttta cttggtggtg 8460
gcgtaggctt tcctccattg ctggatattc caccagatgc tgatgacgaa gctatggtag 8520
aattggcaat tgcattaagc ctacaggatc atgaaattgg aggtgaacaa aatcagaccc 8580
tgcaaagtct tcaagttcaa ttaggtcaag atgtgcttgg cgcccaccaa ggccaaaatg 8640
cgcaagcgca agaatctgct aattttagcg acacaacagc ttcggcagct ggatcagatg 8700
atgaaggttc aacagctgca actgatggtt caactttgag gacttcacct gcagaacaag 8760
ctgggtctga aagtggcggt agtggagttg aaagtattac tggagaacac aatgtttctg 8820
gaaggtcttc cgcatatgga gataatatgc aagaagcaat caatctagtt tctagatcag 8880
ataccagttc gattgctaac acggttgccg gtatggttga ggctgatgcc cagcacgaag 8940
aagttgaact ggaaactgaa agtagcagta gattacacgt tctcaggctg caactcctgg 9000
agaaactggt ggagtatctt ccgaaattga aaaacgttga tggagtcaga gtaataccct 9060
tcttacaagt ggtactccag ttaactgcag atatcgatgg ccattctgag cgagaccgag 9120
tatgtttaaa ttcactttta acaacaataa ttagtgaatt acaactaaca aatgataatt 9180
ttgctgacgt ttgcacacga accacccaac gcgaagttca gttgatactt ttgcgattac 9240
tcagtgtatt aatgcaaaga tgtaaaacat catcaacgtc aacatctagc aaaaccccaa 9300
taccagacag cactaccttt gtttctcgca ccacagcaac agctcttcac aaagctgaca 9360
ttatcaacta ctgcaataaa ctccttcaag ctttgttgag ctactggcga aactctacaa 9420
atgaggatga ttcggtcagc atcacaggaa atcttcttaa agaacgattg cctcatccgc 9480
cacctgatat gacaccattt ttccagaggc agtttgttaa agacaatcaa gacgtattcc 9540
atacatatcc acaacttttg acagaaatag ctttgaggtt gccttaccaa gtgcacaggc 9600
attcggatgt ttcagagcct atcagtgccg cgttccatgt gtcatggtac aagcatctct 9660
gtgattatat gatgacacca cagacacctt ttgtaagaag acaagtacgt aaattactca 9720
tgttcatttg tggaaataag gaaacatata ggcaattgag agacttacac ggattaagaa 9780
accatatgaa gagagtaatg gaatgctgta ccaaggctgg ataccaacct gctactgaca 9840
ttcagcatgc tttgagcctg ccgtatgatg ccctggttga attaattgaa catttgaaat 9900
attgtgttga ggtcgctcaa agtcgcaccg gtaactggca acgcttctgc ataaaagaag 9960
aagacgtaat ccaattcttg atacgtatca gttttctttt ggatgatggt gttgcaccta 10020
ccgttcttca gcttcttcaa agcgcaatag tagttaactc accggcaaag aagcccgatc 10080
catcgaaaac agcctccagg aaagatcgag aaaagtctga cgattctagt accgaagccg 10140
tgttcgaaga atctaactct gtaattttag tagaacagat caccaagcat atatctaaag 10200
aagtgtttgc gcgttttgta aagacattta tgctagaaac taatataacc tctgtaagat 10260
ggcaggcgca tgcgttgacg ctagccattt ataaaaactg taaacctaaa gatcaagaag 10320
ccatactaga ccttttgtgg cagttatggc cattgctacc ggcttatggg agaaaagctg 10380
cccagttcgt cgatttgctc ggttactttt cactgagata tacggagaaa accgaaggtg 10440
ttgaagctat tcccgaatat gtcgaacgag cattcagtgt tcttaaagca cagaacgaaa 10500
tgttagcaca tcacccaaat gcaaacctat atgctcacat gggccaattt gtagatctgg 10560
atggatatta cttggaatca gaaccttgcc ttgtttgtaa caacccagaa gtttcgttta 10620
ctgtcattaa attgagttca atcaagattg attcaaagtt tacaacaact acacaaatcg 10680
taaagctact gtccagtcac acaattagta aaatcactat aagaatagca gatttaaaac 10740
gaacgaagat ggttagaaca gtcaatattt actataacaa tagatctgta caagcagtag 10800
ttgagcttaa aaataaacct gcactatggc ataaagcaaa gaaggttact ttacaatctg 10860
gtcaaactga agttaaaatc gagtttcctc taccaattgt agcctgcaat ttaatggtgg 10920
agtacgcaga tttttatgaa aacattcagg cttcatccga aacgttacag tgtccgagat 10980
gtagtgcctc ggtaccggct aacccaggag tatgcgccaa ctgtggtgaa aatgttttcc 11040
aatgtcacaa atgtcgtgct atcaactacg acgaaaagga tcctttcctg tgccatgcat 11100
gtggattctg caaatatgct aagttcgact ttagcttact ggcgaaacct tgttgcgctg 11160
tggagcctat agaaagcgac gaagatcgca aaaagaccgt ttctagtata aattcttttt 11220
tggaaaaagc cgatagagtt tacaaacaac tcatcgcgaa taaacctact ttggaatcgc 11280
tggttgttaa gattacagag cacagacccg ataaaaaaga agatgctgtt tcgacttcgt 11340
cggcaccgac tgcaggtggc gctgcagcag cagctgctgg tgctggagcc cagttgaaag 11400
tgaacaaaac aatacaaatg ctggctcaac aatactgtaa tgagtgcaag acttcgtttg 11460
aggagctcag caaaattata cagaaggtgt tggtgtcgcg gaaagaattg gtggcttatg 11520
acaggaaaca cagagatatg gaattgccga aatctacacc ggtccttagc gaaaatctgg 11580
tcaattttcc ttgcgttaac aatcgttgtt acggttgttc caccgctgcc acagaacact 11640
gcttgacttt gctaagagca ttggcacaca atcctacaac cagagaagtt ctatgttcac 11700
aaggactcat ccaagaacta gtatggaaca atctaagaaa tggaagcatt cacagtcaag 11760
aggaggtgag acaattactt tgcgttctga ctaaggataa caggactgcc acggaagaac 11820
tctgcaacct tttaatggag aggataagtt tgagtttgaa tggccacatc agttctgcag 11880
acttgggtac gagtgttagg catgaaattg ctctcttggc agcgatggtt caaaaagaag 11940
atgactgctg ggagctgaaa ctcaggtgta tgatgaatct gttcctgaaa gcatgtgaag 12000
gatcgagaag tcccttggtg atggaatcta tcattttacc atgtttaaaa atactgcaaa 12060
gtttaatgaa aattccggaa cctggatcta agaaaactaa ggaaaaacca gctccttcct 12120
tgcaactaag cgtgcaactt gaatctccgt atggtgcgat ggttgacctc aataaatttt 12180
tggataaaga cgatgctcat acttttaatg ggtggaaatc aagattccct aaggcacaag 12240
aggcctctgc ctcggccaaa ctcagcaaag aggagaacag aaaatactat ttggctgaaa 12300
aatactgccg caaatggaga agacacatta agaacctata tatccgcgac ggactcaatt 12360
tcgacgatac ctcatggctc aaagccgtca ttttcaaccc aagttctcga ttagcccgac 12420
aagtcgcatg taacatcata gaaatcatgt gcagcagttt cgaacgcaag cgcgacatgc 12480
tcgatctgct aactcatttc ttagcagagt taaatactgc aggcgaaagt gctgccgagt 12540
tccttgccat ttaccaagaa cttatcgcgg agacgccttg gaaacagtat ttaactgtta 12600
acggcgtact tacagtgctc gcgcacctta tcaccatcga gatagagcaa cttcacaggc 12660
tcgaagagac aactttgacg tcagatcttg cacaaggata tgcattgaat cagattacgg 12720
agttgctgtc atatttctta gatgacgtgg ctattagaag acaatacaaa agcagacttg 12780
ttggagcggt gttgaatgga tacctgtctt taaggaggct tgttgtacag agaactaggt 12840
tgatagatga tacacaggaa aaattattgg aactactgga agaaatgact acaggtacgg 12900
aagaggaaac caaagctttt atgtcagtgt gcattcaaac agtagaacga tacagtcttc 12960
aagacatttt aactccggtg ttcatctttg aacgtctttg ttccattata tatcccgagg 13020
agaatgatgt aggagagttc ttcttgactc tggaaaaaga tcctcaacag gaagatttcc 13080
ttcaaggccg aatgttaggc aatccttatt gcagtctgga agctggattg gggcctttga 13140
tgagggatgt caagaacaag atctgtcaag attgcgaatt ggtggctctt cttgaggacg 13200
acaatggtat ggagctcttg gtcaacaaca aaatcatgag tttagattta caagtgaaag 13260
atgtatataa aaagatctgg ttggcagaag gaagtgacca tgaaccgatg aggattgtgt 13320
acagaatgag ggggctcctt ggtgatgcta ccgaagaatt tgtagaaagt cttagtaata 13380
aatcccaaag tgatgtagat aatgaagagg tctataaaat ggccaatgag ttagccgatt 13440
gtggaggact tcaggttatg gtaactagat tgggagcgat accgagcgtc accagagcta 13500
gaccgttgtt acaagtgtta ttaaaattat ttaggttatg tgtgaaagtt aatcgttgtc 13560
aagaggtttt aataaagccg gagttgaagt ccatggaagt atttttacgc acgttgcaac 13620
tttgtttaga tagcgataaa gattctagtc aaactggcgt taccgaacaa ctattagata 13680
ttatggaaac gatcttgtcg aaagcaacga gtgaatccga agaaaacttc accgagttct 13740
cacaaactct aggtagtgcc gaatacgtca aatctttact gtcatgtact aatcagcagg 13800
tagtgaagaa cagttccgtt ctagtccatt taaccagggt attagccgct ttggtttacg 13860
gcaacaaaga gaaaatgaag attttacttg accatttcag ttccgtcctg gatttcaaca 13920
aatatgacat ggaacataac tcagacgatc aacaaaaact cgagatgttc tgtgttttaa 13980
ccaacagcat cgagaagaat gccatcggca gcacgttaaa agattacata atcgctttgg 14040
aaatagtcaa aaatgcctta gaatacataa ctatgcacgc accttgtgtc aaaccgactt 14100
tattaagggt agacagcgac gagttgaaag atttcatatc caaaccggct ttgaagtaca 14160
tcttgagatt tttaactgga ttagctcata gtcacgaaaa aacgcaacta gcaatagctg 14220
ctgcggaaac tattccgatc attcatcgtc tggaacaggt gtcttcagat gaacacgtcg 14280
gatccctagc tgaaaacttg ttagaagcac tctgcacgaa tccggatgtc gcaaagcaga 14340
ttgacgccgt cagagagttt acaagatctg agaagaaacg tctcgccatg gcaatgaggg 14400
aaaagcaact tggtcagttg ggaatgagaa ccaacgataa aggccaagtc actgcaaagt 14460
ctaatatact ccaacaaatt gaagaattgg gcgaggaatc cggtttggtg tgttgcatat 14520
gtcgtgaagg atataaatat caaccaacca aagttttggg gctgtacacg tttacaaaaa 14580
gatgtaatgt tgaagaattc gaaggcaaac caagaaaaac tgttggatac actactgtca 14640
gccacttcaa catcgttcat atcgattgtc atatgagtgc agtgaggttg gcacgggctc 14700
gtgacgaatg ggaatctgcg gcccttcaaa acgccaacac caaatgcaac ggactcttgc 14760
cgctatgggg tccacaagta cccgaatcat catttgccag ttgtcttgcc agacacaaca 14820
catacttgca ggaaagcacg aatcacagag atataggaca caattcaact attcacgatt 14880
tgaagctttt gctgttgagg tttgcacaag agaaatcttt tcatgaagat acgggaggcg 14940
gtggaccaca aagtaatatg catttaatac catatctgat acacgttgct ttatatgtga 15000
taaatactac aagagtgaat aaaagggaag aagccaacct tatgtcgtac atcgaagcta 15060
ctaatgcaga aaaatggata gaaacagcgt atgatgctga aggtcctcta tattggataa 15120
cgatgtccat tttacttcac tccagacaac agtggcaagc acatcggctt tctcacttca 15180
aacggttaat ggttttggca caagtgcgac attgtcaacc gtccggacct gtcaaaactg 15240
tatcagacaa atcagtaaaa gattataata tctataaacc gtacttgttg ttctttgggc 15300
ttatcgatgg aatctataac catttcttca agaatgtggg aggtccagac gaacaatggt 15360
caaccaactt agcagactac attcgccaca atgatgaagc tttgatgaaa tcttcagaaa 15420
agctcttggc tgattataca gatgaatttt taccttgtac ttccttttca gaattctgtg 15480
atgtggcagg tttactcgac attataacca cgccggatag gtatttaagt gacctgctga 15540
atggattacc ttaatgtgta taaaatgttt ccgaatttta aaatgtaaac aaaggctgta 15600
cactgttttt actaatcgct tgctgctagg ttgtaatcta aataaatgta gtatatattt 15660
gcttgttgtt ggagttttag aaaaactaaa ttattgtaat tacgattgat taattattat 15720
ttgaattaaa 15730
<210> 114
<211> 852
<212> DNA
<213> 玉米根虫
<400> 114
aagaaacaaa ttgaaattga aaataaacaa aaataaaaaa taaccagtcc aaaattaaat 60
ggcaaatatt acaatagtaa aaaacaaaat atgaatgtgc tccagtctca aaacgttgcc 120
agagagatga tgttttacgt aagaccggtc tgactgttgg aaaggtagat ttatattatg 180
gaagaggcgt ccgaaatgat ggatctcttg tacccccgat tagacaaact gcaagtattt 240
ttaaacagcc cgtaactgtc tataagctac aagagagtaa agtaaaaaat gattataaaa 300
acgggaatca agagaaacca aagcaactaa tttgggagaa aagattggaa ggtttaaggg 360
cttgtgatat ggatggcttt gagttcgaag ctatggaact gccaaaaggt ttaaaaccag 420
tgggacctaa tgtaactgaa gacaccatta tccagagtgt tgctacagca ctccatgttt 480
cttctcaacc agtgacaggc cagacaagta caaaaacact acttgaaaaa aatccaggag 540
tatttctcga tcccaaacaa ccattagtac acgctgtaaa tatatcagag gatgatatca 600
aacgtcaaga ggagagagtt gcattagcga ggaagaagtt acaagaggct ctaaagggca 660
acctataaga tataagtatt ttgagtaatg ttttatatgt acatgtcata attattagat 720
tgtaagttac aaattaaatc gttccaatcc tgtaaattat aaccaattgg taaataaaat 780
aagtttcttc agtcatatac aaaaattata ctttttaata ataagaatgt caaagacttt 840
atatgtgtga ac 852
<210> 115
<211> 1099
<212> DNA
<213> 玉米根虫
<400> 115
ctaacaaatt tatacgtata gaaatatacc ctgtgatgag agatgttagt ttagacaaat 60
agaacaacat agggataaaa ctcttctcgc tccggggtaa cggcagagct ttcacaaccc 120
gaaatgttac ctcggttaaa ataataataa tggcaccctt taaaattgtt atggttcgtc 180
atggagaatc agaatggaat gaaaagaatc tattttgtgg ctggtttgat gcaaatttaa 240
gtgaaaaagg taaacaagaa gccattaatg ccggaaaagc actaaaaaat gcaggataca 300
aatttgacat tgcatataca tctgtcctta caagagctca gaacacactt aattcaataa 360
tcaaagaaat tggccaagag aatttggaaa ctataaaaac ttggagactc aatgaaagac 420
attatggtgg cctcactggc ttaaataaag cagaaacagc agcaaaatat ggagatgagc 480
aggtagctat ttggcggcgc agttttgaca ttccacctcc accaatggaa cctgaccatg 540
cttattatga taccattgta aaagatgccc gatatgctga tggtcctgca ccagatcagt 600
ttcctaaatt tgaatcctta aagctaacaa ttgagcgtac tttacccttc tggaatgaaa 660
ctgttgttcc acaaattaag gctggaaaac agatcttaat tgcagcacat ggtaacagtt 720
tgagaggaat tgtaaagcat ctagaccagc ttactgatga ccaaattatg cagttgaatt 780
tgccaacagg aattccattt gtctacacat tagatgaaaa tttgaaacca ataaagagtt 840
tagaattcct aggagatcca gaaactgtga aaaaggctat ggaagctgta gctgcccaag 900
gaaaagccaa ataagcatta tttattattt attgttttaa tttatatcaa aatcatttat 960
tgttagatat ttgatgtgta atgaataaat ggttaggctg aattgtaaaa ctcagcagaa 1020
atgttatgtg caagacatta aagcatattc ttagataaga aaaacatgtc tgaggatgat 1080
aaaatataaa aaaaaaaaa 1099
<210> 116
<211> 523
<212> DNA
<213> 玉米根虫
<400> 116
caactagcgt gttaagactc tcttggtctc ctgatggtca gtatttagtg tcagcccatg 60
ctatgaatgg tggaggacct acagcgcaaa tcgtagaaag agaggggtgg aaacatgata 120
aagattttgt tggacacagg aaagctgtca cttgtgtgag gttcaactcc aacattctcc 180
aaaaacaaga aaagaacaac tctaaaccca ctcaatattg ttgttgtgcc attggatcaa 240
gggacagatc tatcagtgtt tggttaactt cattaaaaag acctcgtgtg gtcataaaag 300
acttatttaa taatagtgta ttagatatgt catggagtag caatggactc tatttgatgg 360
catgctcttg ggatggttct gtagcatgta ttgtgtttgg acatgcagaa attggaacac 420
ccttgactat ggatgagaag aatgaattat atgaaaaaaa tgtaccacaa atcatttcaa 480
aaaaagttgg aatcgtaatt ttggtacgtc ccaaatcatc gaa 523
<210> 117
<211> 531
<212> DNA
<213> 玉米根虫
<400> 117
attcccaaga aaatagaacg aaatctggtg aaacatggat aaggataatc acccaaggtc 60
aggaagtgac tattgactgc gatgaaaaga ctttagcaga aatgggattt aaagataacc 120
agatcgttta tatatcgata ggcatgtcga ggaatatgaa gaaacgggag tttatggatg 180
caccatcttt acacccgcct cctcctcggg aatgtttacc aacattactt cttctacagc 240
caaattactt tgagcagcta ttttccttaa tgcacacact tagttcaatg aaaattcaaa 300
ttaaaggagg gtgccaatta ccacatacaa gagcccaagt tctcagcaga agagtatggg 360
atattcttag cttattacca accagtccca aacttcttcg tggtttcaaa tacttggata 420
tccctcttcc agaattgtta gatccttcaa gtgctcaaaa attaatgtat tctttgtata 480
ttgtcgaatc tttaagcgcc aaaatgtacc gaggaaagta ttcagatgag g 531
<210> 118
<211> 4324
<212> DNA
<213> 玉米根虫
<400> 118
gtagaaaagg caactgtcac aatcgatatg agaagcgctt tcaaaaagtt cgtagacact 60
agattattgc atatgaaggt tttaccatcg cctactagaa cagctttacc caaatgcgaa 120
ctgtgggatc cagaaactat gaccagtgca ctcgatgtcg taattagagc agcaaaacac 180
tcgtatcccg agccagtaat gttagtgaga ggttttagcc agccagtcaa agttagtttt 240
gtgtatagtt gtaaccgatt ctatgttcag ttagtaaaaa aagaacagga actaacaact 300
cttatggaag agcttcaaag tttatgtcta gaaagtgctt ttatggactt ttcttccgtt 360
aaaccaggaa ttccatgttg tgcccaatac agcgaagacc agatgtggta tagatcgcaa 420
gttacagaag tacaaggaaa tgtagtcaca gttcgctaca tagactacgg aaacgaggaa 480
tcagtggcta tagaccagct gaaaaacatc gaaggcgaaa ttctaactgt tcttaaacct 540
caagccattg aatgttgtct gaatggatac caaaagatgg aacctgatct ccaaagggat 600
ggtttacttg aggaactgct attggaaaac acatttacaa tgaaagttgt ggatatgtgc 660
gacaaaaagg cattggttga cttatttgat gaaaatgaat acaacgtttc ttccttgttg 720
ctggtcaaat tatctgaagc tagttcacag gtcagtcccg tattggtaca agcaggcaac 780
aaactagaac atagaaaatc atatacacaa caaagggaac agaatttcaa gggggacaga 840
agcccaaaca ccggacgtga acaaaaacct tgggaaaaac ctggagacag gaacgaaaag 900
tcttggagac aagatagtcg agataataga gataatcgaa atttcgaaaa aaatacgaac 960
gacaacgata gatcttggcg tcaaaataac aaagattaca gtgtaaatag tcgactggac 1020
aaatacaaac aaaacgatac cgggaacagt aacgactata acagtaacag taacgatgcc 1080
aattggaatg acaatcagac cagcgagagt tggaacgacg gcgccgaaaa taatcccacc 1140
gaagaaccaa aagacgaatg ggtaactggt tcgaacgata acaatgaagg tggatccagt 1200
tggagaaaca acgataacag aggcgataga aagaacagag gggggtctca tagtgacaga 1260
ggtggtagag atggtttcaa gagagacaga gatggaggtg gttataacaa cgatagaggc 1320
ggtcgtggag attataacag cgataggggg gatcgtggag gatttaacag tgatagaggc 1380
gaacgtggag gatttaagag tgatagaggc gatcgtggag attttaacag cgatagaggc 1440
gatcgaggat ttaaaagcga tagaggcgat cgaggattta acagtgatag gagttataac 1500
aacgataggg gcgacagaga cgattttaac agcgataggg gagatcgtgg aggatttaga 1560
aatgatagag gaggaagagg tggaggacgt ggaggtttca acgatagagg aagccaaaga 1620
gatggaggat ataaaagaga ttataataaa aaggatggat ctgaggatgg ttctgagaaa 1680
ggcttccaaa gaggaaacag atcgaattcc cgctatgatc gaggaggtag accccctaga 1740
gacttcgaca aagccaggaa tttcgatgac aacaatgctg gtagagattc atggaactcc 1800
acaccgaaag caccggccat agattctgtt cccgtctcta ccacattcca gtcatacgaa 1860
gtcgttggta ctgaagcgaa gattacagtc agttggttcc ataatcctga actattccat 1920
tgccagttag tagactatca agaaacattc aagaccatga tggaagaaat ccacgacttt 1980
tacaaagaca gaaaacccga attggtagtt tgcggtgcac ctgtagttgc tctgtttccc 2040
gaagacaatg ttctgtatcg cgcaagagta ttggaagttg taggtatcga ttacaaagta 2100
tatttcgtcg attttggcaa cgtatccata gtttccaaga cgtttcctat cgataagaag 2160
tttatgaacc ttcctgctca agctatagca tgttccttga acggcgtaag tcccgttggt 2220
gaagattggg cagatccaga tacatatagc ggatattttg ataaggacgc ttttgtttgc 2280
aattttatta aaaccgttga tgaaaaagct tatgtaaata taacctacga atcccaaagt 2340
atcgctgacc tattagtcag agacggcttg gccctgtcgg ccgcatctgg tccgtcggac 2400
ggagatgtag atatcaacgt gctactcggc cagcaattta gagctactat catcagcgta 2460
aatgatctga acgacttcat cgtgggactg gattgtggta aaatcatcac ctgcaccatg 2520
cataacttgg aaactgcgac tgaaactttt aaagacgacc ttaagggtct cttgcaacag 2580
gctgtgatta tatatgtcga taatgtctta gaagataata agttagaaat taccctttat 2640
gatactgaag gaaataaaca cgttatagtt aaccctgatg aaggtgcata cgacacttta 2700
gatcttcctt gtccaacgct gatattacgt tcaaacataa ccggttacgt acctttcggc 2760
agcgaatcat cagtttttat ccaaccaaac gaatacctcg aaaccataaa ttcccttctc 2820
gatctgttgt ttgaaactta cgacagtaaa ccaaacgaga acacaataat tcccgaaact 2880
gattccatat atgcggtaca tagtgaagat ggaaactggt atcgaggcaa agtgactgat 2940
ttcgatgatg aactggctac ggtttcgttt atagattatg gcaactctga gcaagtggcg 3000
ttttctgcgt taagagagct tgaaaaagca tttttggaca ttcctatttt gtgcgttgag 3060
gttaatattg gcgttgacgg taccccgttc gttgacagtg aagtaacagc caaagtcttt 3120
tatggtgaaa ctggctggga aggtactctg cagcccccgg agtcttctgc tccagaggcc 3180
gcttcagtac cagaaccgca ggaaaattat gtcgaaagta cacaggaaac tgttgcctat 3240
actgaagaga taccacaaga gaatatttcg aatgatcaac cattacatgg tcaaatagtt 3300
gaaacaggat catctgctga agcagcatca gctgttgatg aaccattaaa aggaactgtt 3360
gtttatatga gtcatattga ctccccaagc gatttttacc tgcagtttgc caaaaacaag 3420
gaagatatcg aaatccttca aaatgaatta caaactatgg tagaagaaat ggaggttctc 3480
gaaaatccag cacatggtgc tttgtgcgct gctccctatt ccgtggacca atagtggtac 3540
cgagcagaag tgttggatgc tgatgaggat attaccacgg tgcgattcgt tgattttggt 3600
aacactgatg tcatcgataa taacacaaca aaagtaaaaa ctcttcctcc aaagcttcta 3660
tcactagcga tctacgcaac gagatgtgcc cttaaagttg agcccttggg agaggaatgg 3720
gacgctttag caatgacagc ttttgagaat cttaccaata tcgaccttaa tttaacggcc 3780
gagattataa atcaggatga aaaatgcacg tatgtggaac tgtacagtga tggtacggat 3840
gttcgggcag ctttggtgaa tgacaacctg gttaaaccaa ttgcagaaac gaccgaaaca 3900
agacagactg gtttcgtaag ccacttaaac tcaccgtccg aattctggat ccaactggag 3960
agttgcatag acgaactcga atggattgcc gaacagctca gctcagctga gacattcccc 4020
gacgtaacgg atttgactcc cggcacttta tgtgcagctc ttttcccaga cgatcaaatg 4080
tggtacagag ctagaattct atctgacact gtggcaggtc tagaacttat tttcatcgat 4140
tatggcaact cgtgcgtctg cacggatttg aagcagcttc cagaagattt ggtcatgact 4200
gcgccacttg cgatgaagtg tagtctgcag aagaacgacg gtattcccac atggacgcca 4260
gaggccgctg ctaagttttc ggatatatcc gccgaaggcc agacgatttt taccgttaaa 4320
aaaa 4324
<210> 119
<211> 7780
<212> DNA
<213> 玉米根虫
<400> 119
aaaaaataaa agtgtcgggc gataaaaagt cattttatgt actttttaag acgtcctttc 60
ctctagaaac ccacaaagtt ggtcaaatta tgagtataga gttgaaccta caatgttaat 120
gtaaaaacga atcaaaatat gactattgca actagggcac aaggattgac aggagatagt 180
gttgaacctc aaggatcttc tcctggacca tctgagaaca acgtcgtcga ggaaatccaa 240
gatgaaaact tcgaaccgga gttccccaaa gataaattag cgtcactgga tgaaaaaata 300
tcgagcttgc gatgggtggt gccagtcctg gccgaccagg agctagaatg tctactgaaa 360
gtctccatag acttggcccg aaaaaatctc gatacaagat ccgaatcttg ccagaggttc 420
ttcagggagg gattgacggt gtctttcacg aagatattga cggacgatgc agtgtccagt 480
tggaaaccga atattcacgc ctgcatttat caaaactgcc tgaaactcat agagctgtgc 540
gtggtgaagc tgcctcaaga ctggtaccct ctattggatt tactcgccat ggttcttaat 600
ccgaataaca agttccacag cttcaattct tctagacaga gcgaaacggc cggtccgaat 660
tgtaatttat cggaagagga gctcttcgct agaccatcga gcgacttgag gaatcccaga 720
gggtggttgg tggaccttat caacaaattc ggagagttgg gcggtttcca agttttatta 780
gatagattcc aatcggggaa aaatttaagt atttctatag tgttcgcttt actgaggcct 840
tttgggtttt gctttgaatt tttgacggtt cacagtatca acaagtatat attgcctata 900
ttagaaatga ttcctggtat attagataaa ttatcggacg aagaattgaa gaaggaagcg 960
aaaaatgaac aaaaaagcga tatcgtctcg gctattataa aatcgtctaa gaatttggca 1020
tctagagtac ccaatcaaga agaacttatt agaactttag aaggatttag attgactatg 1080
atattaagac agttgcaaat ctccagcttt aacggaaaga tgaacgcttt aaatgaaatc 1140
aataaagtta tttcgagcgt tatttattac ccgaatcgac accacggaat ggaagaggag 1200
gaatatctca cgcctgaacg tatggccaag tggattaatg aaaataaagt attagaaata 1260
gtgttaaggg attcgctaca ccagccgcag tacgtagaga aactcgagaa gatactgagg 1320
ttcgtcatca aagagagggc gctaagtttg cacgatttgg acgccgtttg ggcagctcaa 1380
gtcggaaaac acgaagccat agttaaaaac gtccacgatt tgttggctaa actcgcttgg 1440
gacttcagcg ccgaacaatt agaccattta tttgagtgtt tccaaaacaa ctggacttcg 1500
gcgtcgaagc gacagagaga aagactttta gaactgatac gtcgattggc ggaggacgac 1560
aaagacggcg tcatggcgca caaagttttg acgctgtttt ggaatctggc acacgccgaa 1620
gatgtcccaa ccgaaataat ggatcaagct ttagctgctc atgttaagat attggattat 1680
agttgttcgc aggagcggga cgcccaaaag acagtttggt tggataaatg tgtggaagaa 1740
ctgaagaacg gagaaatgtg ggcgcttccg gctctcaagc agatccgtga gatttgcacg 1800
ttatacgaac agaacacgaa cggcagccac actcaacgta cccaccacat ttactacaga 1860
caagaagtga tagaacgatt gcaaaaccag cattctctag ttatattagt aaccaacagc 1920
ctttgtagtt atatggatag attaagagaa ttaatcaaag agaacccgga attgaccagc 1980
gagacctaca tacccgacgg acggtacggc cacacgctgc aagtccagga aagactgaat 2040
tttcttagat tcctcttaaa agacggacaa ttgtggctat gcgccgaaca agcgaagcaa 2100
atatggcagt gtctcgccga gaacgcggtt ttccagagcg acagagaagc ctgtttcaag 2160
tggttctcta aattaatggg agacgagcct gatctagatc ccggcatcaa caaagacttc 2220
ttcgaaaaca acatattaca gatagatccg atattgctca cagaaagcgg gatcagatgc 2280
ttcgaacgat ttttcaaagc ggttaacgtg aaggaaggca aagtgaaata caaaagaagg 2340
tcccttctaa cggaagatcc cgatctaata ggtctcgatt atctatggaa agtagttact 2400
ttgtgcccaa acgatatcgc taccagagcg atcgagcttc ttaaagaagt cagtaccaac 2460
ctcggtccta gattgaccca agcgcagttg gtgttccacg aaaactacat caccgaatgc 2520
tacgacaggc ttcgagctca ctacgacacg ttaagtatct tgcaaaaatc gaccaacgac 2580
aaagaattcg actccgagca acttcaaaac cgcattaagt cggaagccgt gaaaatatgc 2640
agggtactga aggttctaca cgagtacatc agcgaatgcg acaacagctt cgtgggggaa 2700
cgtaaaattc ttccgttgca cagagcttgt tacggcaaac acattactct gatcgtgcgg 2760
ttctctagtc ccaacaggca agtcgaagac ttggaactgt acacgcattc gaacgatact 2820
ctagcctctt tgagaaaata cattttgaaa cgaatcaagc ctggtatata ttgcaagctc 2880
gaattgttca tcaacggcga gcctttggag cccgccgagg acaagaaact tctttcgcag 2940
atacccatca gggacaaaat gttaatatca gcaaaaatag ttcaaatgaa ctcgaatatg 3000
gcgtcgtccc aggacagtag ttcggacagt tcgacctctt ccccccacca cccgtacgac 3060
ggtccaaacg tcgaagcgga gaatctgtta ccgggcgttc tgatgtccca acaaccgttc 3120
tacgcccaat tcttctgcca gttgatgaac gtaggaagca cgttgccgtt tgcgatgttg 3180
agagatatgg ggcacgcgct tcttcagttg atgccttgca atctgatgac gatggagaaa 3240
ctgaggattt tgttttctgc gccgggagag cagaacattt cgatggatag catgtttttc 3300
agcgcctcgc cttctgaagt attatacaac ttggaagtcc tctacactat gttaatgcca 3360
gccatagacg ccatctccga aaaatcgtac gaattccagt acagcttcat gatatccgga 3420
gaagcccacc tcttcctcga gatgttgacg aagaataact tcatgtcgag cgccgataac 3480
gttaccagac gttcggcgta tctagttgtc ctgaaaatat gtaaattgat actgacatct 3540
gtcgcgcacg tcctggtaag actgagtgaa gatcacactc ctccagaaaa tgaggctttc 3600
aacgaaaata ccactccacc gggaaccttt ttgagacagg cattacgaaa tgttcctgga 3660
cactcggatc atttactgcg acaagtttcc atgaaactag ctcaaggttt agcgcaattg 3720
atggtatccg aaacgggaaa caccggccaa gctcaagcac tgttcagtca agcgttgaat 3780
tgggaactgc cagacttagc cacgacgttg gctttggtgc ggctggtttg ggcagcgagc 3840
agcaacaatc tcggcgctgt caacgcctcg ttggaaaact tgcactcttt atccgaagcc 3900
agcaacaaga aaaagtcgga actagtcaac gacgacgtgc tcctctgcaa agaagctctg 3960
gaactgctca gtaccgccgt agtgctcaac cccagcagtt tgcagcactt gtaccaggac 4020
aattggtggc cttacttcgt tacggacttg gtgctagtca atccgaattg ttccataaga 4080
gtcgcagctg ccgaacagat gatcattata tgttcttgcg gcgctagcag tcaaatggct 4140
ttgcacgtca tcatgccgtt gctcttttct ctcatcaata ccatcgtagt cgaacacgcc 4200
aacacgtccc acgagttttt tcaactgttg tgccgtttag tcaatgtagc gtaccttacc 4260
ggatgtccca tcaacggtgt ggagacccta ctgtcaaatg aggttgcgtg gttacgaaaa 4320
gccagagaca aacacgaagt actgatagaa ggccatctcc ttctcgccaa agagctcctt 4380
ttgctgatct cctcagaaca gaagtgcgaa ctgggtagtg ctgagtccgg cagtctgatt 4440
aaagaattgc tcgaagactt cctgtttccc gccagcaagt tgatgttgag attacacaaa 4500
acgggtcagc tgggcgaaga cccggccatt cctatttgtg atactcccca aactcaggcc 4560
gcagcattcg atctgttggt atcactgtgc atgaactgtg tacaaaacta caaacagctg 4620
gttaatatgc tcacggagat gttttacaac gatcctgaca ctgcaatctc ggaatgggac 4680
taccttccgg cagtagggcc gcgtccattc caaggtttcg taggtttgaa gaacgccgga 4740
gctacttgtt acatgaactc agtcttgcag caactctaca tggtcgacag tattaaagaa 4800
ggcattttgg cagcagaagg agcagctacg gaccctaacg aggatttcac cggtgaagag 4860
agactggata tggacgtgga ttgcactgat gataggaata gtttggacga taataggaag 4920
gactacaacg ttgggatttt gaagcaggtc caggctattt tcggccattt ggcctgttct 4980
cgccttcaat attacgtgcc taggggactg tggagacatt tcaaacttca aggcgagcct 5040
gtgaatctga gagagcagca ggatgccgtg gagttcttca tgtcgttagt ggagagtctg 5100
gacgaagctt taaaaacatt gggacacgag cagatcatgt cgaaaattct tggaggttcc 5160
tactccgatc agaagatctg caaaggctgt cctcatcgtt attccaaaga agaaccgttt 5220
agtgtgatta gtgtagatat taggaaccac agtagtttgc cggattcgat ggagcaatat 5280
gtcaaaggtg aattattgga aggcgccgac gcttaccact gtgaaaaatg cgcgaagaaa 5340
gtagtgactg ttaaacgact gtgcgtgaaa aaacttcccc cgattctagg tatccaattg 5400
aaacgattcg aatacgattt cgaacgagta tgcgcgatca aattcaacga ctacttcgag 5460
tttccgcgcg aactcgacat ggaaccgtac accgtgtccg gtttggccaa aatcgaaggg 5520
gaaattatag actgtgatct tcaacctacc tccaccgagg tctgtaccaa gtatcgtcta 5580
tctggtatcg tcgtccattc tggacaagct tcaggtggac attattattc gtatattaga 5640
agtagagatc cctcgggaga cgtcagatgg tacaaattcg atgacggcga cgtgtcggag 5700
tgtcgaatgg gcgaagacga ggaaatgaag gtgcaatgct ttggcggcga ttatatgggt 5760
gaagttttcg atcccatgtt gaagaggact acgtatagga gacagaaaag gtggtggaac 5820
gcctatatgt tgttttatac caggcatgat gttgaggagg agagtgcctt gaagatgatg 5880
aaccaattga cgatttccgg tacgagaaaa gaaacacatc tcaagatgcc gatagccata 5940
gaaaacagta taagaaagca aaatatcaaa tttctacacc ataggagtca attttctttg 6000
gaatatttca cgttcatcag aaaactggcg acgagttcag ctcagggcaa tactagactt 6060
agtcaaccgt tgccaaacga tcaattagaa caacagtatc tactcagtgt tcaactagtc 6120
agcaaattcc tttttcatac tgggtggcat acgaagaaga atctacgagg acctgccatg 6180
gaatggtgtg acgtcctatg tttgcatctg cgcacctcgc ctgtgatacg ttcctggttc 6240
gcacactgca tgctcttcga gcacatgagc cgtttctgcg aatacctgct gagttgtcct 6300
agcaacgaag tccgttctgc atttatcaag atcgtggtat tgctcgcgca cttctccatc 6360
aacgacggac cgtcggcttc tccggcggcc ctcaacagca ccgccactgg ctctttgagt 6420
gatcacatcc tgtgggcgtt gctcagtctg ctgcagaggg aggtcagcga gcacgggcgg 6480
catttgccgc actattgtac ggtgtttcac atgtacgcca atcaaggtat tcaagaaaag 6540
acgcagctgc tgaggatgaa cgtgccggcg acctttatga tggtcgctct agacgaaggt 6600
cccggaccag ccataaaata tcaattcacc gaattaggaa aacttaatca attagtcgct 6660
tgtttaattc gatgctgtga tgtaagcagt aagtgccaga acagtaccaa cggtccagtt 6720
ttacccaatc cctacaaaga tcccaccata caggaataca tcatgccaat ctcctcgcct 6780
gccgccgaaa tcctattcaa caggaacgta tacgtcaaga aagtgatcga agatacgaat 6840
ctgactgaag acgcgatcaa attcttgcag ttctgttcgt gggaaaatcc tcatttctcc 6900
agggcggtat tatcggagct cctgtggcag atagcctacg cctattgcca agaacttaga 6960
caccacatag agatcttatt gtcaattttg ttaatagaag attcctggca gaatcataga 7020
atacataatg ccattaaagg tgttccggaa gagcgggaag gtcttctgga aaccatcgtc 7080
cgtgccaaga atcactacca gaagagggcg taccagtgca tcaagtgcat ggtggcgttg 7140
ttcagtcgtt cgtcggcggc tcaagctatg ctgttgagac aggccgaagt gaggcgatcg 7200
tggacctcag cagtagcttg gttacaggac gagctggaga ggaagtaccc cccaaatgct 7260
cagtactcct acaacacgtg gtcgccaccg gcacaaagca acgaaagctc caatggatat 7320
tttcttgaaa gatcaaacag tgcccgcaag acgttagaga aggcgctaga actgatgcct 7380
gagaccgaaa gagaagagga agtggaagag acgcactcgc aagaggagcc gtcgccgccc 7440
tccgagcagc attcgcagca gcacacgatc gcgcagccgc aaaatgtcga taacaacgcg 7500
ccgtcagagc tgccggatgt aacacagcac tgcgaaaacc agcaggacag gtaaaagaaa 7560
tgacggacgg gacggcggtt gtcgagcatt tcatccactt ctataagctt tgcacgttat 7620
ataaaacggg cgataggaat gtgatatgta tgtatatatt ataaaaaaaa aaaaaaaaaa 7680
caggttatat tggctgggaa gcatttttct cttgagcatg ttcctcatat ttttttggtt 7740
tttaatccgc aagtctaaat gtaaataccg aggcaaaaaa 7780
<210> 120
<211> 1219
<212> DNA
<213> 玉米根虫
<220>
<221> 尚未归类的特征
<222> (1198)..(1200)
<223> n是a、c、g、或t
<400> 120
gtttggttta catcttagtt tttaaaataa aaaaatgttt gaagctcaag aaattcacgc 60
agaacataaa gatctcattc acgatgtcgc ttatgattat tatgggagga gaatggccac 120
ctgttcaagt gatcaatttg tgaaggtttg ggatcaaaca tctgatggaa agtgggtgtt 180
aacatcaagt tggaaagcac acagtggatc tgtatggaaa gtaacttggg cccatcctga 240
atttgggcaa gttttagcca cttgctcctt tgacagaaca gcagctgttt gggaagaaat 300
agttggagaa ttaccaggac caggcgaaag aggtaccaga cattgggtaa ggagaacaaa 360
tctagtggat tcccgcactt cagtaacaga tgtcaagttt ggaccaaaat ctcaaggact 420
ccagttagct acttgttcag cagaaggaat tataagaatt tatgaagctc cagacgttat 480
gaatttgagt cagtggacat tacagcacga agtccaatgc aagctgccat gcagttgttt 540
atcatggaat cctagtttgt ctaaaatgca cccccaaatg atagcagtgg gaagcgacga 600
tccaaatcca gcaaatggcg gcaaagtgtt catctatgaa tacaatgaga acagtagacg 660
atggacaaaa ttagaaacta taagttcagt agtggatcct gtacatgaca ttgccttctc 720
gccaaatttg ggaagaagct atcacattct tgctgtcgct accaaagatg ttaggattat 780
taatttgaca cctgtaaacg atgaacagag cattcagagt ggagtaacta aattggatgt 840
agaaaccatt gcacagtttg aagatcacaa cagtttagta tggagagtat gctggaatct 900
aactggtaca gttctgtgtt caactggaga tgatgggtgt gtcagaatgt tcaaaatgat 960
ttacattaac tcatggaaac ctgtagccgt attgaaaggg gactcctctc aatcctcgta 1020
cgatcaaaga accaccagct caacgaacaa cggaagtacc atcataccat tttaccaaac 1080
tgctagatat atcaagttag gatctatagc acagacgagg gaggcaccgt ggcactgaat 1140
ttattataac ttatttatat tgttaactga tgattttaat ataataaaca ttttaagnnn 1200
taaaaaaaaa aaaaaaaaa 1219
<210> 121
<211> 680
<212> DNA
<213> 玉米根虫
<220>
<221> 尚未归类的特征
<222> (21)..(21)
<223> n是a、c、g、或t
<220>
<221> 尚未归类的特征
<222> (506)..(506)
<223> n是a、c、g、或t
<220>
<221> 尚未归类的特征
<222> (585)..(585)
<223> n是a、c、g、或t
<400> 121
tcaaaaggtg ctaatgtcat nttcaaatga gtgaaaagag cgtgggaacc atgcaggggg 60
tttcgggcga acgccggttg cgccaactgg aaatgttgtt tcttggcggg cctgtgctag 120
ccaaagaaca aagttttagt atcgaaaccc tcatagatat attacttgtt ctctacgatg 180
aatgttgcaa ttcgagttta aggaaagaaa aaacagtttc agattttata gaacaagtta 240
agcctgtagc cagcacagta aaatctttaa gattgactcg tgaagatttc gagataatca 300
aggtgatagg tagaggagct tttggcgaag tgtgcgtagt caaatttaag ggcaccgaaa 360
aggttttcgc catgaaaatt ctcaataaat gggaaatgct caaaagggca gaaacggcgt 420
gttttaaaga agaacgcgat gttttagtct atggtgatag aagatggata acgcatcttc 480
attatgcgtt tcaggatgta tctaanttat atttagtgat ggattatcac tgtggtggtg 540
atttgttaac tttattaagc aaattcgaag atcgtcttcc agaanaaatg gctcggttct 600
atatagttga aatggtctta gccattgatt caatacataa tcttaaatat gtacacagag 660
atatcaaacc tgacaatgtt 680
<210> 122
<211> 5553
<212> DNA
<213> 玉米根虫
<400> 122
tgaagagtgc acagtggtgt atggagaaaa ctgctgtggg tacgcggatt gaatgacttt 60
tatgtccctt ttctgtaaat gagtgttttt tttgtataaa gtaacctaag ggaactaacc 120
gttgttgttg actaaatcgt atttgtgata taactcgacc acatgaggcg tatcctcctc 180
aagcatcagt gattaagttt tacctatcga attattggtg gatcaagacc cggagaacca 240
tttgtagcgg ttggctatta ttttgggtta atcattatca cgatctcata atatgacgga 300
tacgagtcag gcggagcaac cgccccaaac gaacggcatt tccgacttgg aagacgatga 360
cagggccaaa atgagaccag tagacataga cgccgatgtg agagaaatgg aacgaaggaa 420
acgagtagag atgataatga acagtaaact attcaaagaa gaactggaaa ggatcttaga 480
ctcacagctg aaagatggat caggaccaag cgggcttctc caacaaattt cggacatggt 540
aggcgtcaac agacaaggcg gaaacgtttt taagagttcg agctgtgtga ttcccgttaa 600
cgacattaga ggaatcgagt cgatgggata tggcaaggga gaaaagattc ttagatgtaa 660
attggcctgc ctctacaggt tgatcgactt gtacggctgg gcctacggct caagtacact 720
cataactgcc aggttgaacc aggatgacga acaattcttg gtcaaccctt acggaatgct 780
cttccacgaa gtgacagctt ccagtctgat taaagtagac atgcagggcg cggtcctcga 840
acaaggcaca accaacttct ccgtcaacat taccgcatat tcactccacg cagcagtaca 900
ttccgctaga ccagacatca aatgcatcat ccacgttcac actccttccg tagtagctgt 960
atcttcgtta aaacaaggac tgatgccttt gagccaggag tctgttgtca taggtgaagt 1020
aagtacacac atgtacttag gcggtcatct agatccagaa gaaaaggaca aaatcgcaag 1080
gaacctagga ccgaacaata aagtcctcct actgaccaac caaggagcat tatgttgtgg 1140
aacgaccatt gaagaagcct tcttcaatgc cagaaatacc gttttagcct ctgaatcaca 1200
gctcaaactt cttcctgtag gcattgataa tttagttttg ataaatgatg aagtaaggaa 1260
aaaaatatac gacgcagcgc acaaagcacc agaaagtact ccccgaccgg accaaccctc 1320
tattttggag ggcaaagtag aaagaaagtg gcgtgtaggc ggtgtagaat tcgaagcttt 1380
gatgaggatg ttggacaacg caggattcag gacaggctac atctacaggc atccattagt 1440
taagggcgaa ccaccgaaac caaagaacga cgttgaggta cccccagcag tttcctccct 1500
tggatatttg ttggaagagg aggagttata tagacaagga ctctggagaa aacttgaagg 1560
tggtaggaaa ggcaacgatc gcagcagatg gttgaattcg cccaacacct atcaaaaagt 1620
ggaaattttg gaaaccggaa cacccgaccc caagaaaatc acaaagtggg tagccgaagg 1680
atcacctagc cattcgagca gcacaccagt taaaattgaa tcggctcttc agttcgtacc 1740
aacaggtact aatccaaagg aattcaaaca aaaacaacaa cagatcaaag attacagacg 1800
agcagacaaa atttctgccg gtcctcaatc ccacattttg gaaggtgtaa cgtgggagga 1860
agctaaaaaa atgcaggacg ctacagtaac tcaaacaggc gaccatgtag tgctaatggg 1920
agctgcttca aagggcatta tccagagagg gcaccaacac aacgccatgg tatacaaatc 1980
gccttacgcc aagaatcctt tcgatagcat cacagacgaa gaactcaacg aatacaagaa 2040
agtcgtagaa aagaaagcta agggtgaata tgacactgac tacagtgaat cagaaggtct 2100
ttcgtcagca gctttgaaca aaagaattga agatatgcaa attaggtctc caacttctgt 2160
tactagtgag acagaggaag aaagtagaga cgaacctcaa atcttacgga tagaaacgaa 2220
gaccgttccc aaacctagtc aaccagaagt tgtattaagc gacggacgaa ctcgaagaga 2280
actatataga actaacaact atgcaggttt tcccaaagga tcatttttgg agcctaaccc 2340
cttgcatttc cacttagtca gagaaagcca gatcgctgtt agacagcgcc ctctaaagat 2400
atctttgact actaataata gatcttcaga ttcccgttct gttgtcgtta accttgataa 2460
ttctaggtgt atatctgtaa acatgccttc cagtacattc gagggcttta ttgttccgtt 2520
tgcatcagca ttgaactcaa gaaatcttcc tatgatcaag gaacaagctt cacaaaccag 2580
ctatgttaac gtagtgaatt atactccttt atatattagt caatatagaa catgtctgac 2640
agaacccgac aatacaaatt cattcgagag aaatattttg atgaacatgc acgagcagat 2700
gctggaagat ttgaacgata gctcagattc tgagtatgag tctttaaata acaccaaaca 2760
gcaatactgt gaatctacca gaagggatat tgttgagttt gatgaaaatg atcagtgcga 2820
caatgagagt ttggttgaag taacagcaga ggtacaggaa atgatgttaa atatagaaag 2880
cagcactgat ttaaatgaag atggtccacg tgagaaccaa cgttcggttg cagtagctgc 2940
agaattatca gaaacgactc cacctacaga aagtttttct tctgtttatg ttgaatatga 3000
cgaaaatgat caatttgaga atgaaagttc tgttggggga accacagagt catcagaaat 3060
tctgactaat gtagaaagtt gtactaattc ggaaactgta ggaatatttg ttgatgtagt 3120
caagcaagca tttgtaatga cagaagactt cttaagtgga gccaaagata ttttaatgaa 3180
aattcctaat gacagatata ttgtcaaaga aaactctgat gaaggaattg tggattgtac 3240
ttttacgaac aaaaccttca gtatcgacga tgataatgaa aatacaactg tattggaaaa 3300
aactgagtat gagtatgttc aagatttgac acaactgata gtggactatt taatagagca 3360
gtgtatggag gttaaagagc ttattctatt cccagaggtt ctacatgatt ctaacttgca 3420
taaattgctt gaaaagatga aattaatata ttatgacgcc tttaaggacc agttatgtga 3480
agataaaaat gagctaagac tttctttagc ttcatcagtt ctaaggagac tggaactcga 3540
agaaaagtta aacaattctg gagattctgt aacctcgaac acagtttccg ataaaatctt 3600
tacgatttca gagtttttga acgatatttt ggaccatttc tttgacaaca tcgatttaag 3660
gcagtttaac aacttcagtc aggagttaat tgcgaacagt gatcttgaag tacattctac 3720
accaaaactg aaaagaagag atataaatgg caacgttgag ccattcgaag tgaatacctt 3780
tcatcaacag accttgtcaa agtcgggctc tgaaatattt tggatttctg tgtctcctgg 3840
tacttctgct aacaatgaac aacctcgtag aaaaattatt aatgttgatg atattccttt 3900
gagacctcct gttgacctga ttagtaaaga agtgttggac cctatatctc aacctcctgt 3960
tgacttgatt agtaacaaaa tgttggctgc tatacctgaa gaagtttgtt tagatttatt 4020
taacacttcg tcaattgaag aaggatttaa accagttgat gataattcca ctttagtaaa 4080
aggacttcag acgcaaaaag gagataatac ttgtgtcaac gtagagaaaa aaaaagttac 4140
taaaattttg actaaccaag tgagttttta taggagccaa agtgtaaata gtactgatta 4200
tgttagtgta aaaagagcag tatggaacaa caatactgaa aataaggaaa atgacccctt 4260
gaacgattct ggagattgga tgggttacga cactgcaatg ttttgaattg aatttcattt 4320
tgtatatcct ttaccaagtt ttatttatat ttgatatatt tctcgttttt aaatacattt 4380
ttatatcaaa gtgtatattt ttcttcgtat gggggaagtt tttaggggag attaaggttt 4440
gaacacaatg tatagtaatg cacaataaat aaatccacta acattgaata tagacatgta 4500
tacaacacat tttttaatca aaatcactaa tcagtatcat caagaaaagt ttcgacctaa 4560
tttcatcatc aggaatcatc tacctcatgt aagaaaatct aaaattatag ttaatttata 4620
tcctccctct aaagttatgt cttcgtgtcg attttgataa caatttataa atataaagtg 4680
aaataattta tcacatttat ttacaaaaat tatatcaaca cactgtaact ttttcgacag 4740
aaacatttta ttgagtaata tttggtcctc tcactccttc atgccatctc tcttgaggtg 4800
gtaaacagta cggaaacggc tgttctcttt ttcatatata ccacgttttc cttcaatttt 4860
tacacagtta aactaaactg tggttttatt tttcacaagg cgattacaaa catttggccc 4920
ttctctgttc tttcatttat aagttgaatt ttttctattt ttcttttaag ttggactttt 4980
ctttttacat attcctgttt gtttacgttt tttatcaaaa tgtccccatc ttttgcgtct 5040
gatagtatac caaagtacta agaataaatt attgtaatca atttttgaaa acacactgat 5100
atgaaggata aactgttttt tctgttttgt gcattttttg tttgtactgt atacaatttc 5160
ggatacaaaa tcatttcttt tatcggtgtt taaatctatt taatgtttta ctgtaagctc 5220
aatctcctgc ttggttttat ttagtttata gttttttctt ttcaaattaa aataaataga 5280
taaaaatcag ctgagcctta accactttct tgcattttcc taatttattt gtagtttagt 5340
agtctagaat aattgtttga ttaatcttta aaaattgatc agttagtaaa ttttgtgttc 5400
acgccttaag gtgtatatta ataatgtata taatgtaaaa gttattgacc ttataacttc 5460
tcttttattg tatttaacga ggaaactgta ttttaaacaa tgtttactat gtaaaatctt 5520
attcactaat aaaatatttt tcgtcaatta aaa 5553
<210> 123
<211> 978
<212> DNA
<213> 玉米根虫
<400> 123
cggaatcatg ttatcaatgg aaaataaact aaagaaatac gaaaaaatta aatttttagg 60
agaagggcaa tttgcaacag tatatcaagc tcgagatgta gacacggaca atatagtggc 120
tgtgaaaaaa attaaaatag gtagtcgaca agaggcccaa gatggaatca atagaacggc 180
tcttagagaa attaaactac tccaagaact gcatcatacg aatataatcg gtcttctaga 240
tgtttttgga catatgtcca atgtgtcatt agttttcgat tttatggata cagatcttga 300
ggttattatt aaagacaata ctattattct cactactgcg aatattaagt cttacatact 360
tcaaaccctt catgggttgg aatatcttca tttgaactgg gttctacata gggatttgaa 420
accaaataat ttgttagtta attcaagtgg catcttaaaa gtaggtgatt ttggtttagc 480
taaattgtat ggatctccca ataggataaa tactcatcaa gtcgtcacaa ggtggtacag 540
agcaccggag cttctttttg gagccaaaca gtacagtacc tgtatagata tgtgggctgt 600
aggttgtatt ttagcagaat tgctgctcag ggtacctcta tttcaaggag aatcagatct 660
ggatcaattg acaaagattt ttgatgtatt tggaaatccc acagaagaaa actggcctgg 720
gctaaagaca ctatcagact tcatcgagtt caaaccattc aacgccattc cactgaagtt 780
gatcttcacg gcagctggtg acgatttgtt agaactaata cagggtttat tagtgttaaa 840
tccaatcaaa agaaaaacat gttcagaatg tttgcagatg cctttcttta gcaataagcc 900
cgctcccact attggtacca aactaccact tccacagagc ttaaggaata acagagataa 960
tgataaacct actgcctt 978
<210> 124
<211> 1132
<212> DNA
<213> 玉米根虫
<400> 124
cctttcatgc tctgttatac tcaagaggat gctaacgcag aaggcaacga ggaaaacgtt 60
ctatcgtacg aaccagtcca acagttgcat attaattata cgagacctgt tattgtactg 120
ggtcctctaa aggatcgtat caatgatgac ttgatatctg agtttcctga caagttcgga 180
agttgtgtac cacataccac aagacctaaa agggaatatg aagtcgacgg tagagactac 240
cacttcgtcg catccaggga acaaatggaa agagatattc aaaatcatct attcattgaa 300
gcgggacagt acaatgacaa tttgtatgga acttctgtag catctgtacg tgaagtcgct 360
gacaagggta aacattgtat cctggatgtt agcggtaatg caataaaaag gctgcaagtg 420
gcgcagttgt atcccatcgc tgtttttgtc aagccaaaat cggtcgaatc cataatggaa 480
atgaataaaa gaatgacaga agagcaagcc aagaagactt ttgaaagagc aatcaagatg 540
gaacaagaat tcggcgaata cttcacagct gttgtgcaag gcgacactcc cgaagacatc 600
tataaccaag tcaaagaagt aattaaggaa caatcaggac caaacatatg ggtaccggcc 660
aaggagaaac tgtgaccttt ggccccaccc atatggactc ttccgtctcg tccacaacgg 720
ctgcactgaa gacgccacta tttaaactaa tataagatcc ccaaaatccc cctgtagcga 780
atgagcgcta aaagttttca aaattgcgca ctgatttttg atcaaaaatt gtcctcagga 840
gtatttgttt attcatgaag tattcttttg aaatttgttc tgtctactta tagtttattt 900
cactttgttt ttgattttgt tttattattt tagattcata tcggtaattc ttagtttggg 960
taaattagtt tccagaaatc tctaagagat tgaaagacat tcatttttac atatttctgg 1020
gaaaatttgt ctattataat ctttctcttt agaaagtgcg tattttgaaa tttacagttg 1080
gtcgaatcga tcggttattc tcttcgtgtc cgctacgtat ttacgatctc cc 1132
<210> 125
<211> 1151
<212> DNA
<213> 玉米根虫
<400> 125
aagaggagga agaagttgaa attaagattg aagaacatga tgtgaaacct agcgtcagca 60
gactttacca tgctgccgag agcgatcata gctaccacaa gggcaagcag agtgccatgc 120
agatggctac ctttggaatc gagactcctt ctgattcaga ggaggaaata gatgttgtca 180
atgtcaacaa cgagaagttc ccgttcaata aaacggcggt gttttcgttc cccaataatc 240
catcgacgaa agatcgccaa cagatccaga tgcgtatggc aactgctata tccaagaagc 300
gaatacaagc ccaagctcaa ggcatcaaga ctatcatgcc cgttaggaaa acagccacac 360
cggaggcgtc acctatcaaa aggaaacccg tggaaaccac aaggagtggg aaaaaaactc 420
ggcaatacag aacaccggtg agcactccat acaaacgtag acaatacggc aacagcagcg 480
atagcgaacc agaaccatcc gagaagagaa gtttacacaa caacatggag aggcagcgaa 540
gaatagactt gcgcaatgcc tttgaagatc tgcgagtatt ggttcccgaa gttagcagaa 600
agagagagcc gccaaagtgg tgatcctcag ggaggcagct caatattgcg atttcctcac 660
caacacctcc acaaaatatt cgaaacactt cgatgaatta aaaaagaagc aggaattcct 720
taggcggaga gtgtcacaac ttcgaaggaa tttagcagca aatcgttaag taaatataga 780
atttgtaaga aataatgcga tttgtagtaa agggtgtgtt tagaattgta tagaaaaatt 840
ccgagtatcg gagagactaa tgagtgactc aaaagagcga aacatctcag ttatttgtta 900
tatttttggt ttcaaaaaac agaacttgtt ctagcaacat tatatacatt atcttaaatt 960
ttcagtctcg aaagcactaa taggcaaaag caggttgttt tatcctgcga tcattgtgga 1020
tagctgaagt gatcctgagt tcaaaaatac aacaaacaaa aagatgttac gtttaccagt 1080
cgtattttat accttacatt aattgtaatt gatttttgaa tgccgttttt gttttttcga 1140
aatttggtca c 1151
<210> 126
<211> 1529
<212> DNA
<213> 玉米根虫
<400> 126
ccgcctgatc ccgcagatct ttccaaggga atggaacaaa ctcctattcc ggttgtgaat 60
ggaatcaata gagaaatgtt agacttttgt aattattcaa ttaaacgagt tcctatggaa 120
ggcgttgata tgaatctcga tccagactat ctgtgcggct gtgattgcga tgacgattgc 180
attgataaga cgaagtgcgc ttgctggaag ctcactttag agggagcaaa gtataccgga 240
aaagatgtaa atcctacatc ggtcggatat atttatcgaa ggttgcccga acaagttatt 300
acaggaatat atgaatgtaa ctccaggtgc aaatgttcat cgacgtgtct aaacagagtg 360
gtgcagaacc ctatgagttt gaaacttcaa gtatttagaa ctcacaatcg cggttggggt 420
atcaggtgtc tcaatgacgt tcctccaggc agctttatct gtatttatgc tggtacaata 480
cacactgaag ctatggcgaa tgtggatggt gtggcgttcg gagatgaata ctttgcagaa 540
ttggattata tcgaaaccgt tgaaaaatat aaagaagatt acgaagcgga agtcatggag 600
gaggaggaag aacaaaataa gaataaacgt cgaaaggaac ccgaagagga agaggaagaa 660
gaagaggaat ccgtagcggt gatctctaac agcaaataca aaaggtacaa ttatgctgca 720
gataacgatt ttacacctag tcatcccgac aggattgttg cgggagatgc accaattaga 780
acgcgtctaa gaaagagaaa caaagatgac gctaaagatg aagagaacga agtgaaagga 840
caaaaaagaa gactcaagga atgaaatgat agaaaataaa acaagaaata tacaaataaa 900
aataattaca gagtctaatc aatcaatgaa ggcattaatc gaaggactgt ctgataatat 960
cgaaactgtt accataagcg acgaagaaga tgagggaaga gaagtattaa gttttaatcc 1020
acaagcgagt aacttggacg acaaagcacc caattacacc tctgtccgag atctctacgg 1080
aaaagacgaa tctgtttatg tgatggatgc aaaaaacgct ggaaacattg gaagattttt 1140
gaatcattcc tgttcgccaa acgtgttcgt ccagaacgtt ttcgttgata ctcatgatcc 1200
ccgtttcccg tgggtatcat tcttttcgtt gcaccatatc agagcaggaa cagaactgac 1260
gtggaattac aattatgaca ttggcagcgt tcccggtaaa atgttgacct gtcattgtgc 1320
ctcaggagaa tgtaaagggc gtttattgta atctgacaga atttgtactg ttctgcaggc 1380
aatggagatg tattatttca tacattacgt aattatttat accgtttata atagtgtgct 1440
gtattttatt aatttcgtgt atcaaaattc aatatttcgt tcattgattt tgtctttcat 1500
tgtgctaagt attagaataa tatataatt 1529
<210> 127
<211> 2613
<212> DNA
<213> 玉米根虫
<400> 127
aaacctactg tgtaggttcc tttgtttttg ttaatgagaa caagtggtgt tgtattgagt 60
gaacaagtga aaaatattga gtataattaa ttattcacat acaactggtt tttgttttca 120
caactggaat acatatctta ttctttaagt taataatcaa gttcaacaat gtggcaaacg 180
caggcagtcc atattaccac aaacaataag gagaaccact ttcaaaaaag tgaagataag 240
ttaactcttg gaatgacttc tgctatatca acagcagaac caaaaccagt tgatttggta 300
aaaacaaggg aattaataga ggctctgaaa ccttttgatg tattcgaaag tgaacaagaa 360
ttaaacaaga ggatgcacat tttagggaaa ctgtttagtc tagttaaaca atggataaag 420
gaggtttcac tttcaaaaaa tatgccagag agtgttgctg agcaagttgg cggtaaagtt 480
tatacattcg gaagttacag attaggggtt cataataagg gggctgatat tgatgcactc 540
tgtgtagctc caaggcatat tcataggtcc gatttctttt cctcctttta tgaccttctt 600
aagcaacagc cagaagttac tgatctcagg gcagtagaag aagcatatgt gcctgttata 660
aaaatggact tcgatggtat agaaattgat atgctctttg cacagttatt acttaaagaa 720
attccagaat caatggattt gagagatgat atgcttctta aacatttaga acctaaatgc 780
gtgcggagtc tgaatggttg tcgagtgaca gacgagattt taagactggt accaaatatt 840
gaaaacttca gaatggctct tcgagctatt aagctttggg caaaacgaca tggtatatac 900
agtaacgtat tgggttactt gggtggtgta tcctgggcga tgctagttgc aagaacatgc 960
cagttgtatc caaacgcaac atcatctaca cttgttaata aattcttcct tatattttcg 1020
aaatggcaat ggcctcaacc agttctacta aaacaaccat ctaacgtcaa cttaggattt 1080
gtggtgtggg atccgagggt aaatgttcaa gatagatacc atctcatgcc aatcattacg 1140
ccagcttatc cacaacagaa ttccacattc aacgtttcac aatcgaccag aacgattatg 1200
gttgaagaat tcaaacaagg tctgcttatt accgacgaca taatagtagg caagtacgga 1260
tgggataagc tgttcgaaca accgccattt ttcactaaat ataaacattt cattttactt 1320
ctggttatgg cagataattc cgatgatcat ttggaatggt gtggacttgt ggagagtaag 1380
gcgaggttac tagtaggtca gctggaaaga aaccaataca taaccctagc acacataaat 1440
cccgagagct atgccatgct tgaatccgcc atggaacccc atacattatg ttccatgtgg 1500
ttcataggac ttgagtttgc caaaactgaa ggtatcagcg tcgatctaac aagagacatt 1560
cagacattta cggaacaagt caataaccat gctatcaata tcaagatgct gaaggatgga 1620
atgaggttag acgccaggca tgtcaaaagg aaacagctgt cacaatatct tgctccggga 1680
gtgacttaaa agagaaagga aattgagtat cagtaagaac ggggttacac cggaacaaaa 1740
tagaaagagg cagtcttcgg aagctactca agaacacaac gatgctccaa gtaaaaagac 1800
gaggttgtca gaggatatcg cgtcacaaga gttcgatgaa tgctccaaca cgtcaataca 1860
catagattcc agcagtaatt taagtctaga atcagagagt aacattagtt gtacatcccc 1920
ggcctcaact ccctcccata taaatacaaa ttccaccacc acaaacgtac cagaaacggt 1980
gtgtacgtga caacctacaa ctgatgtata tgtgagtcgt ccattcgttg ctgtcaaata 2040
aaataatttt tattgtaggt ttcttccaag aaacaacagt ttatgtggtg ttcacgtatg 2100
tattaaatta cttttccaaa aacagacaga aaaacttttt tgaggtaaaa tattatcgca 2160
aagagtgcaa tgttcttatt ttaaggctta atgtgtttat tgtgagtgtg cgttaaaaat 2220
tatcctgtat atacaccaaa aggattcctc cgtcgtcaag gaaagataat ttgtaggcag 2280
atagaccgac tgcacatgaa agcggatgat ctaaaatcca acgaaaatac acaggatgta 2340
tcacttgtaa aatgttcgtt tatttgttta tcagtttaag gattctttaa tggtttagat 2400
ggatctagcg atttagttaa cctatggagt aatttataat tgaaaacata agtttatgag 2460
gagtttaaag gtattgtctc attttcttct atttttagtt gtataccatg gagtctgata 2520
ttttttcggt ttctcgtcca ataactaaat tgtcgttcta tatttccctt tagtttcgtc 2580
aacactcata acagtctgcc ctcttgtgtt taa 2613
<210> 128
<211> 450
<212> DNA
<213> 玉米根虫
<400> 128
atgaggatag atcaactatt taatatcatt atagaatacc cagaatctct accggcttta 60
gaagacctac gaatatgtct tcctagaaca gatctaaagc ctatgctaac taagaaacta 120
caaaaagcaa tggaaacacg attactacac cctggtgtaa gtacacctga tgttctaaca 180
gcttacgttg ctgccattag gtctttaagg gtgcttgatc ctacaggact attgctcgaa 240
actgtcacgc aaccggttca tgaatacttg aggagtagag aagatacagt aaggtgtgta 300
gtcaccagtt taactgagga tggtcctaat gatctagcag aggagttggt gagaggagag 360
gccgttcaag ttgatgaaaa tacaccactg gacgaagata atgaagactg ggaaacttgg 420
gtgccagatc ccatcgatac agtaccaaat 450
<210> 129
<211> 1812
<212> DNA
<213> 玉米根虫
<400> 129
ttgatcagaa gagacaatat atcacatgaa tagaataaga agaaagtgaa tgatatgaat 60
acgtccctga cgtttataaa atttgacatt ttacaggaat tttgcacgaa tttactagaa 120
acatggtttt aaataacgtt aaagaactaa ccgaagaaga aaaattcgat attcagctgc 180
aagctgagca ggaactgggc agactagtca gaacttatac gatcctagaa agaaaccgag 240
cccaaaaatg tgatacaggt ggtaaacttc tcaaacaacg aaaagtgctg gacattttca 300
agaaggaaca aaaaaatatt ctcacggatc ttgctgtagc gtctgccgat gctagaagag 360
tggaggacga aaagaagtcc aaggaattga aggatttact tagagagcac gacgatttcg 420
acgctgaaat cactcaacaa aaatcacata ttagtgagat cgatggacag attcgaatga 480
ccaagaaaaa ggttcttgac ttaaggtcga agcaaattac agacgaaatg tatcaacaaa 540
gagtaattgc aggagaaaag acggttcaaa ctttagaaaa caaactggaa gtccaaataa 600
agaaattttg ctcaatatca gccgagaata tcaagttaag agaagaaatt gatcatttac 660
ttttagaaag aaacgatttt aacaaaattt gggacagcct tatcaacaat ttagctgtcg 720
gaaagaagtt catgttggac ttaatagaac aagcaactat agcttatgat caaagggaag 780
agtgggtgtc caagctacag attttaagaa caaaagccca caatgatctg attaatcaca 840
ttcaggaaat gagggtgttg caaagaaaaa tggacgacga tcagaaacta caagagtttt 900
tctcagtgaa atgccaaaaa agaatcatga gacaactaga agaaaaagaa aaaaacaaaa 960
ggatgctcaa taaagaaaat atggaaaaga aattggaacg ttacttacaa attttaatta 1020
cgattaagga atttaccgga gaagaaaaag ttacgacgat tgccaacaat tttgtaaccc 1080
aagaagaaga aaattttgcg atgtttaaat atattaatca tttgaacaaa gaaatggaag 1140
atcttacgga cagtctagca aaattgcaac tcaaaattgg cgagcaggag gcactaaatg 1200
aattgcgtaa gcaccaacaa gaaactaggc tggaaaagct gaataaagat tttgaagatg 1260
ctaaacagat cacgagtcaa aaatctgaag agctgaaagt aattgatcaa aagttgagaa 1320
cgattataaa tggcatcggc caattgttta gaatgtttag atgcaaaaat gatcctctaa 1380
taaagttatt aggtgaaaat caaaacatac attattataa tgtactgctg tatctggaaa 1440
ttttggaaaa caacatcgaa gaggcaatgg tttctgtcaa tttcaaggag acccaaactt 1500
tggaaagaac aaaacacaag gaacccaaaa taacaacttt gagacacgtg aaatgtccac 1560
ccatgatcga acccatcgag agaatagtcg ctactaatcc ttgccctttg tgcgttgaac 1620
atgagcatgt cagtgatgtc atagatgtac tgcaattcgc gcttaccaaa gaagagatcc 1680
aggagaggct taagatcaaa atcgataaaa cagaggcaga cgacaagttg cataatgtat 1740
ccgcctgtca tctgccgaaa tccagacaaa ttatacaaaa aagatatcaa taaaaattat 1800
aaagtgatca ct 1812
<210> 130
<211> 830
<212> DNA
<213> 玉米根虫
<400> 130
tttttttcat ttttgacacc gactttgtca tttatttaac aattatatac taattttaat 60
attacgattt taattgatat aattatatct aaaattcaat aatggctaga tacgatttga 120
cattgaatcc catggaagaa accaggtttc ttaaaaaaca tagagtactg gctttaagtt 180
tggcaagaca ggtccatttt agctttcagg aggttgaagc cattctcatt atatattaca 240
agatacaaaa atacgacact tacgatccaa aaggtattac gagagacgtt ctgatggaag 300
ttttccattg ctgtttagat atgacggata tggaaaaagt gattcgaata ataacttgcc 360
tagacgagaa agctcgagat gtctacatat caaaagagct ttggtgcaac atgttatcgt 420
tatttttaag aggaacgatt gaagaaaaaa tggcattttg tttcaaagtc tatgattaca 480
gtggaagcgg acatctcatg aaggaaaatc tttttaagct tttagtggga tctttgaaga 540
gcaacatcgg cgaatcagat gcggaagaag ccgtgaagga tatggtggaa gttataatga 600
agaaaatgga tgcagaccgg gacggcaaga tttcctttaa tgactaccga tataacgttc 660
tccgccaacc agacctgttg gagtttttgg gccagtgtct tccagacaga catacggttc 720
acaaattttt gtgtaccttt actgaaaata ctaagatccg attctataag ttaaaattag 780
gcacaagtat aatgtgaaat taggaagtaa acgattaaaa gttaaaaaaa 830
<210> 131
<211> 2072
<212> DNA
<213> 玉米根虫
<400> 131
gttaaatatg atacatcaca ccaattcgag gattataaca actacaggtt aactttacgt 60
taaagataac ttcggtcatc catcacacgg ctgattagct atctattgac aggttgtgta 120
tatagagcag ttccttgtaa cacgacttgt ctcttctatt ataatgtttg tctcactatt 180
ccaatcagtt ttgaagagtt tttaagccca cacttcataa catttggtag atgaattcct 240
caatttaact atgaggataa aagtacgcat aaagtcacaa aaaatttccc cgcttttccg 300
tgacatcctt attccgcaag ttatattgtt tctgcgcttc ctatagacat gggatttgga 360
ctaggagtcg agctcatcga gctcaaagta tcttctttca acctcaactt attcggatcc 420
ttctcctttt tttctttatc cttatgttta tgttcgtgtt tgtgtttatt aacgctaaaa 480
gacgtactat tttaccgaaa tgatgttatt actacaataa gttaatgatt tttgaacaaa 540
aaataagttt gtttggtgtg ccttctacat aatttttaat tataagtttc atatagaaat 600
aaatttttaa tcatggccgc gccaaggaga gccgcacaga ccgtaaatca agaaaacgta 660
aattcccgtc ttgctgtgaa atcaaacgta aatgcgccat tagaaccgct aaaaagacca 720
gcacttggcg aactcggcaa tacactacaa gacacccaaa aagcacaaaa cggtcttgct 780
ccaacaaaag atggttttaa aaaacctgaa ggtactttgc aacccaaaaa agagacaatc 840
aaaactttga ctaacaagga aaaggcaatt ccgatcaaac ctagaactat cctaaaaaga 900
caggaaagtt tagtaaaacc gttacaagga aaatgtacac agcctaaaga agtaccacgc 960
acatcgacac tagtaaacaa aaaactgtct atcgttgacc cagacgaaag tagcaaaaat 1020
gatcctcaga tggtaaccga gtatgtaatg gatatcttca aatatcttag agacatggaa 1080
ttgaaattag ctatcaaaga taacttcctc aaagaccacg aaactacttc caggatgcgt 1140
gcaatactcg ttaattggtt agtcgacgta cacgcaaact ttaaagccac gcttgatact 1200
ttgcatatat gcattggaat cgtggataga tatttacagg cgaacaaaaa agtaggaaga 1260
aatacgcttc agttagtagg ggcctcagca atgttgatag catgtaagta tgaagaaata 1320
tatgttccag aattagacga ctttgaatat gtatgtgatc acacatttac aaaacgacaa 1380
attcttcaaa tggaaaggga aatactgaag gggttagatt ttagtttagg tcgaccgatt 1440
tcggtgcagt ttctccgtag atatacaaaa gtgactcaat ctcgcgtgga gcaccataac 1500
ttaggtaaat atatgttgga attggtatta ctcgaaagcg atctagtgca cgtaagacct 1560
tccctactgg cagccgcagc ttgttgcttg tcaataggaa tattgaacga aactatggat 1620
ttgccgaaac tgtggaaccc cacctgtgta cagtacacgt cctacaatta caacgacttc 1680
aaatcgataa taacggaact cgcttatctc ttagtcaaaa gcgaacagtc gaaattccaa 1740
gcaataagaa gaaaatacgc cctagcaaaa ttcggcaaaa ttagtcttaa tgttaaatta 1800
aatggtcctc ttgtgagaaa gttgacagcg gggaaaaagt gatatttcca ttttatcttt 1860
taaccgaatt tttatatttt atatcagttt tatatttagt gtcattttta tttacgtttt 1920
agtacatttt aataaattag attcaagcta aagactatta aaagccttca atatcaattt 1980
ttgttagtca gtttttattt gacggaatgt gaagcaacaa tggagcaata aagttaagta 2040
gtccaggctg tttgctcgcc cccgtcaggt aa 2072
<210> 132
<211> 1517
<212> DNA
<213> 玉米根虫
<400> 132
gacagttaat acgcgaaaaa gaactaatac accgatgaaa ctatcgtatt aaaataaagt 60
ttaaagtttt taaattatgt gtggattagt ttagtgttta gtgttatgtt gtgttagtgt 120
tttttttctg tgactaagcc tacaataatt tcttcgagca acaggaaaaa atttaaaacg 180
gagaggttct gaacatttgt atctgaaata aattaataaa gatgaatcaa acgtgtaaag 240
tgtgtggaga accagcagct ggtttccatt ttggtgcttt tacctgtgaa ggatgcaagt 300
cattcttcgg acgctcctac aacaacctaa gctcaatatc agaatgcaag aacaacggcg 360
aatgtgtcat caacaagaag aaccgcacag cttgcaaagc atgtcggctt cggaaatgtc 420
ttttagtggg tatgtccaag agcgggtccc gatacggtag acggtccaat tggttcaaga 480
ttcattgctt actgcaagaa caacaacagc aacaacaagc ccatttagcg aatgtttcac 540
agaacatgaa accaccacag aagacgccac cattacatcc gcagccgcca ctaggtatgg 600
gccttcttgg aatgaattcc tttcaaccgc cacttttaca tttacccaaa acaaaggaag 660
agttaatttt attgggcttg gatgaataca aacactcagc ctctcctcaa gtcagttctc 720
ccgaatcaca caattcagac tcatcaatag aactaagcga tgctaggagg ttacctttgt 780
ttcctggact attacctcct acattcctgc cacctcctgg tctccttttc cctccaggat 840
acccccctct ataccctggc ttactgcaac ctgccaataa caacagactt atgagaaacc 900
acaatcatgg tgtcgaagca tttaacaagc gagtgttcct ggatgcagtc cttaaatctc 960
agcggtcgcc aactcccgaa gaaagaataa ctcccatatc cactgaaacc ataatccaag 1020
aagatcctat cgatctcagc atgaaaacga acagcgatag ggggtcttct ccagctcata 1080
gcgaccaatc gggctcagga acggagcgag gaaatggtag cgaagccgat gaagaaagtg 1140
attgcgagtc agatagagaa atcaaaagga tgaaactcca caggcccacg ccgctagatc 1200
tcacgacgaa agtatgatgg aacatatttt tcatattact gatattgctt tcggagtacc 1260
tgaataatct agataaaaga ttttcaggtc actgatcagt ttctgggcca gtcttgaagc 1320
tatctggatt tccgtttcga aactgtcgat ataatgcaga tcgaaaatga aagtgttcaa 1380
tcttttactc aagaagtcac ttggcggttt tcttaataat atttagattt aaaatggaaa 1440
aagtaaaaag aaccagtaga atattaagtg tttacatttc attgctaaac tgattttggt 1500
tatttgtgat tgatttt 1517
<210> 133
<211> 3192
<212> DNA
<213> 玉米根虫
<400> 133
tacacgtttt gctacttttt gtattatttt ttccaagtgt attaaggata aacctttcac 60
gggattaaaa tttcgtgaca aacaggccta ctattacaag aaacttcagt gttgtgttta 120
cttcactata caagtttgca actatgggta caaatactaa ggacgacttt tcagaatcaa 180
aagaatacgg cgtaaaatct gaggaattcg aagacttttc tgagactatg ttagatggaa 240
gcgataagaa atcattagag gacggctcat tgtcagatcc tgataactgt ggcatgtgtg 300
gtggcccatt ggttatacct cgaatgctgc actgtttaca tacattttgc gaagaatgcc 360
taggcaaaaa gcttgtcggg gaagctggag atgctggatc cacagacact tcaatccaat 420
gcccgatctg taactattca accctggttg gaacaaagaa gttagctgct ctaccgcttg 480
atgtctcaaa gacaaatgtt accgacgttt ccaactcccc cgatttgcat tgcacttcct 540
gcactgccaa agaaatggcc aagtccaaat gtaacacctg ccaacacctt ctatgcaaca 600
attgtgacaa ggctcatcat tttatgagat gtttcgagtc acacaaggtt gttgctctcg 660
aggacatggt taaatctggt attaagatca cagttcacaa gcctctagta tgcgatttcc 720
atcctggcga aaatctcatc ttctattgct tggtgtgcag tgtaacagct tgcacggagt 780
gtattaaagt tgatcataaa ggccaccagt ttgaggggat actcgactct gaaccaagag 840
ttagacaaga gatggagggt ttggttaaga aaggcaaaga aaaaatcgaa aacctcacca 900
aagcttctgc tactttgaac aataacatgg aagaactttc ccaccagcgc tccaccgcta 960
gagatcttat caatgaatca tatcagagtt acaaggctgt tcttgagaaa tgtaaggatg 1020
atattttaga agagctgact ggattgtatc atgaacgtga gctagtcctc atgcaagaga 1080
gtaatgagtt gggcaatacc attgttaact tagaagatgc ctgtaaatgc acttccagct 1140
tattggaagg cggtactgca caagagatga tgtatttaag aacagcactt gctactaggt 1200
tgatgacttt aaaccacaaa tcacccaaac tggaaaaatc attcagtatt gagttcaaat 1260
ctgacttgga taagtttgaa tatgctatca ggagtaattt cggaaagttt gctacagagg 1320
aagatgtcat cgtgaaaaca tcacagtctt ctccagtttt acctgaattg gctcccttga 1380
acatcaacgg gatcaatgga atcaacaact caccacctgc tatcgtcaca aacggttgta 1440
gcgcaccttc ggtaacaaca accagtccaa tttctttacc cacatccatg caatcttcat 1500
tcgacgggga acttggaaac aatttacaaa tgttgatggc tcagagtcct cccttacccc 1560
acgtcaattc aaaccaaggg ttttcccaag ggttttctag tattgccgag tacaatatag 1620
cacaactagc cactttagca gaaaacacaa acagtgctgc cacgtcacca actacagctc 1680
cgttcaatat tgccgatatt ttgaacaatg atactgcctt caagaatatt gcaacgttag 1740
ttaagctggc aggaaccaat actactccgt cgattcctag atccaacaaa gttagtccta 1800
tgcagatccg ctgcaagttt ggccagttgg gacccggcaa ggggcagttc aattctccac 1860
atggattttg cttgggattg gaagaggaca ttatcgttgc cgatacaaat aatcatagaa 1920
ttcagatctt tgaaaaaacc ggaacattca agtttcaatt tggcatccca ggtaaagacg 1980
aaggtcagct gtggtatccc aggaaagtag cagttatgcg caataccggc aaatacgtcg 2040
tatgcgatcg tggcaacgaa cgctccagga tgcagatctt cactaaaaac ggccatttct 2100
tgaaaaagat agctatacgt tacatcgaca tagtcgctgg cctagcagtc actgtcaatg 2160
gtgagattgt cgccgttgac agcgtcagtc cgacagtatt catcataggg gagagtggag 2220
atctgctgcg ttggttcgat tgcagtgatt atatgagaga gccatccgat atcgctattc 2280
atgggaagga attttacgtt tgtgacttca aagggcacaa cgtggtagta tttaacgacg 2340
acgggcaatt tttgcgtaga atcggatgtg agaacctaac caactttccc aacggaattg 2400
atatatcaga tgcaggagac gtattgattg gagattccca tggaaatcga ttccacgttg 2460
cagtcttttc acgcgatggg ggcttgattt cggaattcga atgtccatat gtaaaggtct 2520
ccagatgttg tggattgaaa atcacatccg aaggttacgt agtaaccctc gccaaaaaca 2580
atcatcatgt tctggtattg aatacgttat acatcctgta aaccccgaat aatatagcgt 2640
cgtcgacgaa tattttgacc ttagtccctc ttcccgacca actgtttcat gaatatgttg 2700
tgttagttga catcctctga cacatgttgt gttagttgac aaccctgaca cattctcacc 2760
cgaccctcgc ctgttgagct gtggcattta acaatttttg ataaaaatat ttttatgtat 2820
ttaatcttca cagttcgtac ccagaaatag actaagaact ccccgtaaaa atatgagtag 2880
ataaggtatc ggtacgacac aaatcaaaat gtgcgtagaa gaaagttaac attgtgttga 2940
aacttacgct ggtcacggct tttgttacca gctgctcaaa taattatgct tatattcgta 3000
ttaacgttta cactgttctt taaaaactat ttaaacattg atataaggtt tgttccaaca 3060
gaacgaggaa ccgtcatgta acgattatgc tcatgcgcag taagcaaaat atcccattgg 3120
aacgtattat tttgtttact gcgcagtaaa gcgtcattgt cacatgacga tttttcgttg 3180
tgttggaaca aa 3192
<210> 134
<211> 1578
<212> DNA
<213> 玉米根虫
<400> 134
tatttttaaa cacagatttg aaatgtggga ctcttttact attgatgatt gtcaaacatg 60
tccgtataat agcactacaa atatatatga ccaaatggaa gttccacact tggacaagga 120
aaatattcag ccgttcaatc acataaaaaa tgacgagctg tctctatacg acaatggagg 180
cagtatcaca aaataccgcg aagcaccttt gtctcatact caccgacccc tagaagaaca 240
cgactcaaat tcgcaagatt ctggctacag tgcaagctac catggagaaa aatttttgtc 300
ttatgcctct ccagtacgaa ctgcaagcac atcctttggc tctatggtct ctatggaaga 360
tgaatatttt gatttctctg atgttgaacc tttggagaaa ccaaacttac ctcacgattt 420
caataagctc ataacaaatc ctttgattaa cagaactaaa cctgaagtag tctcatctcc 480
aaaagacact atcataagac cacttttcag aagggcttta tcacttcagc ctatcagtga 540
gaacacacct aatagtagaa gggtccgaac aagtttattt agaggtgaac atgaaatcag 600
atcatttaaa agacctgaac ctccgaatga tctagacagt acaattgtga aaaggtcaaa 660
gatatttaac gataatgatg atagtgattg tgaagcttca gttcctgtag ccaggaccag 720
acctatacta caacgcgcat tctctgctac agaagacagt attatgtgcg cagtgcaaag 780
atctgcaatt gaacctgacc ttattggaga ttttacaaag aacttcagtc ttccgttaac 840
aatcagtcga caccaagatt taaaagccat cacggcagat acactagctt cactgatgag 900
gggagagttt agtgatgctg tggattctta caaagtcatt gactgcaggt atccttacga 960
gtttgatggg ggccacatta acgatgcact taacttctac accaaagagc aatgcatgca 1020
actactaaac tcaacacctg atgttgctga aggttcccac gcaagacata tattggtttt 1080
ccattgcgag ttctccagcg aacgtggccc taatctatac cgatttttgc ggaaagaaga 1140
tagaaacaaa aacgaaagtg tatatccttc tcttaacttt ccagaaatct acctcctgga 1200
aggtggatat aagaagttct ttgagactca tccagagttc tgcactccag ttgcatacaa 1260
agaaatgctg catcctgatc acgaagatga atataggcac tttcgatcca aatcaaaaac 1320
gtggaactgt gattcacgcc aacgtctatt gaagaaaagg ttaggtgtgt aaaacttgtt 1380
taggatgcca tataacttta tagtacaaat aattagctta tctatttggt tcagttcata 1440
gtacctgttg tcaaaaacac gagatatatt attttaatca ttcagacagt tcatgtctga 1500
tgaaccaaaa tctccacacc acttagtggt ctaggtgaag tgccttttac tattatttgt 1560
tgcctgatac cagaagaa 1578
<210> 135
<211> 2465
<212> DNA
<213> 玉米根虫
<400> 135
cgtttagtag tacatagaga aatcgctttt agttttaatc ttttctgtgt tggaatattg 60
aacaaattct aatgattcgt aaaataacac ttctgttaaa aaccaaaact gatagtttgt 120
tcaaaaaaag tacaattttt ctgggcttgc tactgaaata atgtgccata ctctaattgt 180
ggaacaaatt gtaggtatat gttgaaagtg aacaaataac taatgcttac aataatgtcg 240
acatcaagtc aaaataatgg taaaaagagt tcaagcagct ctattgttaa caccccttcc 300
agtactccca ttaccaatct tgcaaatggt agtagtagta cccaaggaac agcacatagt 360
tcacctccac ctaataacaa tgctccaaag tatggtaccc tagtgcctaa cagaatattc 420
gtgggaggaa tatcagctaa cacaacagaa ggagaattga tgcaactttt tagcaactat 480
ggtactgtta aagctgctaa aattatacag gacagggctg gtgtgtcaaa aggatatggt 540
ttcatcacct ttgagagtga agatgatgct aaaaggcctc ttagagaagc tgaaaacata 600
gtgctaagag aaagaaaact taatatagca cccgctatta aaaaacagcc ttttagtcga 660
gcatttgatg catccagtcc acctgctgtt gctgcaaata gtgccagtca attctttttt 720
cctccgggtg ctgccgtgcc atattttcag agtggagtta catattatac tcagccagct 780
cctgctgcac ctggagatcc cactgctcaa caacctgttt atcaaccccc tccaatgtat 840
cccacgcaaa ctggtcctcc acaggcagct acataccctt ctatgatgtt tcctgctcaa 900
actatatata tgccgcaaca gtatcccatg cctattccgt acgagtacaa cttctaccag 960
ggtaacggac cgtcgctgcc aactcagtat ctggccggag gccaaagcgg tccgggaaac 1020
tctcattgca cctcattacc atcatcaaac agtccaccca gacccaattg ttatggtcaa 1080
caggtgcctg cctataattc aggagatccc gtgtactaca acttgccaat atacggagcc 1140
actttggaag gtccgccatt atacgctgat gccttcgatc taagcgctag tggagcttat 1200
gcggaggaaa cctacagcgg catgattact caagaaaatg tagaagcttc tcttggatca 1260
tcggtaaatt caactcgtgc aacttccgat aattttacgc aacatatgga tcaatctctc 1320
gttgagtctg attcaaatat tgttaatgtt ggaggtaaac caccagcaca taaatcttca 1380
gagacaattg tttctactcc ttcccagcct caggaggaga gaaactcaca cacgcctatt 1440
gtttcactac tttccataga tcatcaacag gaaaaagact attcatcaat gcaaagcggt 1500
cgtagacgga aacttctgat tcaaagcaat caaaataacg tacccatgta tcctacaaac 1560
ggatacgtca accagtttgt cggatataat cctcaacaac catccccacc ttcattcggt 1620
ccacctctct ataacaacgg ctacggttat tctgaatatc gtcgatttaa cggataccca 1680
gatctcaggt caaataataa tagtaataaa ccgaggagac gaatatacga aaataggcgg 1740
tcgaacgatc attctagccg ttccagtctt cgcacagact caaactcatc ggctagttgt 1800
gttgacgaaa ataaaaacga agaaaaaact tttcgaacta ctgttaacac tcctccacct 1860
gccccatact cacctatgac caatcatcaa ttcaaattta ttaattacac taacacgaaa 1920
aacgtttata atcataagta cgctcacacg tttataatca taagtacgct aacaacaata 1980
attattataa tagaacagct acacacaatg accgatttaa acataccgaa cctttagcga 2040
ataaaaacaa taacagttcc atttacccat taacaaataa tttcctaaat aatagccata 2100
cccaaagtaa ttctgattcc cttccctccc aaaattccca ggtacatgtg acgactacgg 2160
gtcagagctt cgtcccggcg gctatccaag ctcagacgaa acgcaacaag cgttctttga 2220
gaagaagcgg tgccagtgcc ggaggcatca acgaaatcgg cgccggtgac gcccctttac 2280
caggcgaaga atgcgaagat gtctacaaga agctggaaac gcttaagttg taacgaaaaa 2340
gtggtatctt ttcttgtata tttttttcta tttatttaag actgtacaat aatgtaaaat 2400
actagaaatt taatgtaaac taaagaggaa aaatatataa tacatattta ttgaagatga 2460
aaaaa 2465
<210> 136
<211> 275
<212> DNA
<213> 玉米根虫
<400> 136
caaggtggaa ttttaaggtt ataccgcaca cctggttgta cagcagcaaa aaagtttgaa 60
attgtccaga aacttcagca aacatgtaaa aatctaaagg atattcaaac cgaaatatgt 120
tttcatatcg aatcaacaag tgccttggaa gcaaaagacg ttaagatttt gaaatgggtt 180
cttcaagatc ctctccatcc ccaaaattta agcgaaaagg gaacgctggt agcgaaaaat 240
ggtacggctt tggttgaggt aggacctaga tttaa 275
<210> 137
<211> 275
<212> DNA
<213> 玉米根虫
<400> 137
atggtggatt ctttaaagtc cattttcgac gaaactcctg aagatgaaag tctctcccaa 60
aagattttac aatcagtggc tattgaggtc agttcggcag acaaattatc caccaagatc 120
tgcaaagagt gcgtagaaaa agtaactgac tggtcttcgt acaaagaacg atgtctccaa 180
aaccaaaaga aattaacgga gttgttagtt gcgaaagaag gtccagaagt tttggttgta 240
cctgaaatta tacccgaacc cgaagcacca cttcc 275
<210> 138
<211> 275
<212> DNA
<213> 玉米根虫
<400> 138
aatgatcctg aatacgactt catatctgga ttcgttcatt tttgtggaca ggctggacta 60
gttggtatac aaatattgtg gacaaaagaa tccgaaattg ctattaagaa agcaagagta 120
gatagaatgg ctatgaaaat tacaaataaa cgatttctcg acctcttaaa tgctctcatt 180
gatcttacat caaaagattt aacaaaaatg caaagaataa gatttgaaac catggttact 240
atccacgttc atcaacgaga cattttcgac aacat 275
<210> 139
<211> 275
<212> DNA
<213> 玉米根虫
<400> 139
tggctcctcc accatacgca gacctcggca aaagagcaag agacgtattc ggcaacggtt 60
atcactttgg cctaattaaa ttaaattgca aaaccaaaac agccagtggg gtccaattta 120
gtacaggtgg tagttcagac catgaatctg gaaaagtgtc tggatctttg gagagcaaat 180
acaccgtcaa agattatgga ttgactttca cagaaaaatg gaatacaaat aatactttgg 240
ggactgagat cgccatttca gaccaaggta ttaag 275
<210> 140
<211> 280
<212> DNA
<213> 玉米根虫
<400> 140
tgggcaaaaa gaaggtagaa gagagtgaaa gtgaagagga gtattctgta gagaaaatta 60
ttgataggag agttgtagat ggaaaagtag aatattatct aaaatggaaa ggatattcag 120
aagatgataa cacttgggaa cctgaggaca atctagattg tcctgaactt atttctgagt 180
ttgagaggaa tcgtaaaagt aagaaggctg ctggcaaaga aaagaaaaga acaagagata 240
attctacatc ttccgtggat tcaaacaatt catccactaa 280
<210> 141
<211> 275
<212> DNA
<213> 玉米根虫
<400> 141
tgagttttat aattattgct tttaaaaatt tcctacatac catgttgcag ttattatctt 60
ttggtaaaaa aactattaca aatagtttga gtgtgtatgt aaaaactatt ggtggcaaca 120
ctttaactgt agatctagat cctacatggg acattaaaaa tgttaaggaa atagtggcac 180
cacaacttgg attagaacca gatgaagtaa agatcatttt tgctggaaag gaattgggag 240
acgaaattaa aattgaggaa tgtgatttag gtcag 275
<210> 142
<211> 275
<212> DNA
<213> 玉米根虫
<400> 142
tgaagggaga aaatttaccc caacagaacc cagtgccttc aggaaatgaa aaagatgtca 60
gtaacaaaca ggaatcgtct aaaaatcgat cggatttatc agtcagcatt tacgaacaat 120
taacactacc attaagagat ggccgctcag tatctctcga tactggcgct agcggttcct 180
caactgtacc cgaagtgaca cctggtagcg atccttcatc agaaattaat cgtttgtttt 240
taaaggcaaa cacagaaagt ttacaatcgt taagg 275
<210> 143
<211> 271
<212> DNA
<213> 玉米根虫
<400> 143
atggaactgc caaaaggttt aaaaccagtg ggacctaatg taactgaaga caccattatc 60
cagagtgttg ctacagcact ccatgtttct tctcaaccag tgacaggcca gacaagtaca 120
aaaacactac ttgaaaaaaa tccaggagta tttctcgatc ccaaacaacc attagtacac 180
gctgtaaata tatcagagga tgatatcaaa cgtcaagagg agagagttgc attagcgagg 240
aagaagttac aagaggctct aaagggcaac c 271
<210> 144
<211> 275
<212> DNA
<213> 玉米根虫
<400> 144
atggttcgtc atggagaatc agaatggaat gaaaagaatc tattttgtgg ctggtttgat 60
gcaaatttaa gtgaaaaagg taaacaagaa gccattaatg ccggaaaagc actaaaaaat 120
gcaggataca aatttgacat tgcatataca tctgtcctta caagagctca gaacacactt 180
aattcaataa tcaaagaaat tggccaagag aatttggaaa ctataaaaac ttggagactc 240
aatgaaagac attatggtgg cctcactggc ttaaa 275
<210> 145
<211> 269
<212> DNA
<213> 玉米根虫
<400> 145
tgaatggtgg aggacctaca gcgcaaatcg tagaaagaga ggggtggaaa catgataaag 60
attttgttgg acacaggaaa gctgtcactt gtgtgaggtt caactccaac attctccaaa 120
aacaagaaaa gaacaactct aaacccactc aatattgttg ttgtgccatt ggatcaaggg 180
acagatctat cagtgtttgg ttaacttcat taaaaagacc tcgtgtggtc ataaaagact 240
tatttaataa tagtgtatta gatatgtca 269
<210> 146
<211> 269
<212> DNA
<213> 玉米根虫
<400> 146
tgggatttaa agataaccag atcgtttata tatcgatagg catgtcgagg aatatgaaga 60
aacgggagtt tatggatgca ccatctttac acccgcctcc tcctcgggaa tgtttaccaa 120
cattacttct tctacagcca aattactttg agcagctatt ttccttaatg cacacactta 180
gttcaatgaa aattcaaatt aaaggagggt gccaattacc acatacaaga gcccaagttc 240
tcagcagaag agtatgggat attcttagc 269
<210> 147
<211> 270
<212> DNA
<213> 玉米根虫
<400> 147
tgaaggtttt accatcgcct actagaacag ctttacccaa atgcgaactg tgggatccag 60
aaactatgac cagtgcactc gatgtcgtaa ttagagcagc aaaacactcg tatcccgagc 120
cagtaatgtt agtgagaggt tttagccagc cagtcaaagt tagttttgtg tatagttgta 180
accgattcta tgttcagtta gtaaaaaaag aacaggaact aacaactctt atggaagagc 240
ttcaaagttt atgtctagaa agtgctttta 270
<210> 148
<211> 273
<212> DNA
<213> 玉米根虫
<400> 148
tgtcgagcgc cgataacgtt accagacgtt cggcgtatct agttgtcctg aaaatatgta 60
aattgatact gacatctgtc gcgcacgtcc tggtaagact gagtgaagat cacactcctc 120
cagaaaatga ggctttcaac gaaaatacca ctccaccggg aacctttttg agacaggcat 180
tacgaaatgt tcctggacac tcggatcatt tactgcgaca agtttccatg aaactagctc 240
aaggtttagc gcaattgatg gtatccgaaa cgg 273
<210> 149
<211> 275
<212> DNA
<213> 玉米根虫
<400> 149
tgtttgaagc tcaagaaatt cacgcagaac ataaagatct cattcacgat gtcgcttatg 60
attattatgg gaggagaatg gccacctgtt caagtgatca atttgtgaag gtttgggatc 120
aaacatctga tggaaagtgg gtgttaacat caagttggaa agcacacagt ggatctgtat 180
ggaaagtaac ttgggcccat cctgaatttg ggcaagtttt agccacttgc tcctttgaca 240
gaacagcagc tgtttgggaa gaaatagttg gagaa 275
<210> 150
<211> 275
<212> DNA
<213> 玉米根虫
<400> 150
tgagtgaaaa gagcgtggga accatgcagg gggtttcggg cgaacgccgg ttgcgccaac 60
tggaaatgtt gtttcttggc gggcctgtgc tagccaaaga acaaagtttt agtatcgaaa 120
ccctcataga tatattactt gttctctacg atgaatgttg caattcgagt ttaaggaaag 180
aaaaaacagt ttcagatttt atagaacaag ttaagcctgt agccagcaca gtaaaatctt 240
taagattgac tcgtgaagat ttcgagataa tcaag 275
<210> 151
<211> 271
<212> DNA
<213> 玉米根虫
<400> 151
tgggatatgg caagggagaa aagattctta gatgtaaatt ggcctgcctc tacaggttga 60
tcgacttgta cggctgggcc tacggctcaa gtacactcat aactgccagg ttgaaccagg 120
atgacgaaca attcttggtc aacccttacg gaatgctctt ccacgaagtg acagcttcca 180
gtctgattaa agtagacatg cagggcgcgg tcctcgaaca aggcacaacc aacttctccg 240
tcaacattac cgcatattca ctccacgcag c 271
<210> 152
<211> 274
<212> DNA
<213> 玉米根虫
<400> 152
tggaaaataa actaaagaaa tacgaaaaaa ttaaattttt aggagaaggg caatttgcaa 60
cagtatatca agctcgagat gtagacacgg acaatatagt ggctgtgaaa aaaattaaaa 120
taggtagtcg acaagaggcc caagatggaa tcaatagaac ggctcttaga gaaattaaac 180
tactccaaga actgcatcat acgaatataa tcggtcttct agatgttttt ggacatatgt 240
ccaatgtgtc attagttttc gattttatgg atac 274
<210> 153
<211> 275
<212> DNA
<213> 玉米根虫
<400> 153
tgctctgtta tactcaagag gatgctaacg cagaaggcaa cgaggaaaac gttctatcgt 60
acgaaccagt ccaacagttg catattaatt atacgagacc tgttattgta ctgggtcctc 120
taaaggatcg tatcaatgat gacttgatat ctgagtttcc tgacaagttc ggaagttgtg 180
taccacatac cacaagacct aaaagggaat atgaagtcga cggtagagac taccacttcg 240
tcgcatccag ggaacaaatg gaaagagata ttcaa 275
<210> 154
<211> 274
<212> DNA
<213> 玉米根虫
<400> 154
tgcagatggc tacctttgga atcgagactc cttctgattc agaggaggaa atagatgttg 60
tcaatgtcaa caacgagaag ttcccgttca ataaaacggc ggtgttttcg ttccccaata 120
atccatcgac gaaagatcgc caacagatcc agatgcgtat ggcaactgct atatccaaga 180
agcgaataca agcccaagct caaggcatca agactatcat gcccgttagg aaaacagcca 240
caccggaggc gtcacctatc aaaaggaaac ccgt 274
<210> 155
<211> 272
<212> DNA
<213> 玉米根虫
<400> 155
tggaacaaac tcctattccg gttgtgaatg gaatcaatag agaaatgtta gacttttgta 60
attattcaat taaacgagtt cctatggaag gcgttgatat gaatctcgat ccagactatc 120
tgtgcggctg tgattgcgat gacgattgca ttgataagac gaagtgcgct tgctggaagc 180
tcactttaga gggagcaaag tataccggaa aagatgtaaa tcctacatcg gtcggatata 240
tttatcgaag gttgcccgaa caagttatta ca 272
<210> 156
<211> 272
<212> DNA
<213> 玉米根虫
<400> 156
tgtggcaaac gcaggcagtc catattacca caaacaataa ggagaaccac tttcaaaaaa 60
gtgaagataa gttaactctt ggaatgactt ctgctatatc aacagcagaa ccaaaaccag 120
ttgatttggt aaaaacaagg gaattaatag aggctctgaa accttttgat gtattcgaaa 180
gtgaacaaga attaaacaag aggatgcaca ttttagggaa actgtttagt ctagttaaac 240
aatggataaa ggaggtttca ctttcaaaaa at 272
<210> 157
<211> 270
<212> DNA
<213> 玉米根虫
<400> 157
tgaggataga tcaactattt aatatcatta tagaataccc agaatctcta ccggctttag 60
aagacctacg aatatgtctt cctagaacag atctaaagcc tatgctaact aagaaactac 120
aaaaagcaat ggaaacacga ttactacacc ctggtgtaag tacacctgat gttctaacag 180
cttacgttgc tgccattagg tctttaaggg tgcttgatcc tacaggacta ttgctcgaaa 240
ctgtcacgca accggttcat gaatacttga 270
<210> 158
<211> 275
<212> DNA
<213> 玉米根虫
<400> 158
tggttttaaa taacgttaaa gaactaaccg aagaagaaaa attcgatatt cagctgcaag 60
ctgagcagga actgggcaga ctagtcagaa cttatacgat cctagaaaga aaccgagccc 120
aaaaatgtga tacaggtggt aaacttctca aacaacgaaa agtgctggac attttcaaga 180
aggaacaaaa aaatattctc acggatcttg ctgtagcgtc tgccgatgct agaagagtgg 240
aggacgaaaa gaagtccaag gaattgaagg attta 275
<210> 159
<211> 274
<212> DNA
<213> 玉米根虫
<400> 159
tggctagata cgatttgaca ttgaatccca tggaagaaac caggtttctt aaaaaacata 60
gagtactggc tttaagtttg gcaagacagg tccattttag ctttcaggag gttgaagcca 120
ttctcattat atattacaag atacaaaaat acgacactta cgatccaaaa ggtattacga 180
gagacgttct gatggaagtt ttccattgct gtttagatat gacggatatg gaaaaagtga 240
ttcgaataat aacttgccta gacgagaaag ctcg 274
<210> 160
<211> 274
<212> DNA
<213> 玉米根虫
<400> 160
tggtaaccga gtatgtaatg gatatcttca aatatcttag agacatggaa ttgaaattag 60
ctatcaaaga taacttcctc aaagaccacg aaactacttc caggatgcgt gcaatactcg 120
ttaattggtt agtcgacgta cacgcaaact ttaaagccac gcttgatact ttgcatatat 180
gcattggaat cgtggataga tatttacagg cgaacaaaaa agtaggaaga aatacgcttc 240
agttagtagg ggcctcagca atgttgatag catg 274
<210> 161
<211> 272
<212> DNA
<213> 玉米根虫
<400> 161
tgaatcaaac gtgtaaagtg tgtggagaac cagcagctgg tttccatttt ggtgctttta 60
cctgtgaagg atgcaagtca ttcttcggac gctcctacaa caacctaagc tcaatatcag 120
aatgcaagaa caacggcgaa tgtgtcatca acaagaagaa ccgcacagct tgcaaagcat 180
gtcggcttcg gaaatgtctt ttagtgggta tgtccaagag cgggtcccga tacggtagac 240
ggtccaattg gttcaagatt cattgcttac tg 272
<210> 162
<211> 271
<212> DNA
<213> 玉米根虫
<400> 162
tggctcagag tcctccctta ccccacgtca attcaaacca agggttttcc caagggtttt 60
ctagtattgc cgagtacaat atagcacaac tagccacttt agcagaaaac acaaacagtg 120
ctgccacgtc accaactaca gctccgttca atattgccga tattttgaac aatgatactg 180
ccttcaagaa tattgcaacg ttagttaagc tggcaggaac caatactact ccgtcgattc 240
ctagatccaa caaagttagt cctatgcaga t 271
<210> 163
<211> 272
<212> DNA
<213> 玉米根虫
<400> 163
tgtgggactc ttttactatt gatgattgtc aaacatgtcc gtataatagc actacaaata 60
tatatgacca aatggaagtt ccacacttgg acaaggaaaa tattcagccg ttcaatcaca 120
taaaaaatga cgagctgtct ctatacgaca atggaggcag tatcacaaaa taccgcgaag 180
cacctttgtc tcatactcac cgacccctag aagaacacga ctcaaattcg caagattctg 240
gctacagtgc aagctaccat ggagaaaaat tt 272
<210> 164
<211> 155
<212> DNA
<213> 玉米根虫
<400> 164
gctccaaagt atggtaccct agtgcctaac agaatattcg tgggaggaat atcagctaac 60
acaacagaag gagaattgat gcaacttttt agcaactatg gtactgttaa agctgctaaa 120
attatacagg acagggctgg tgtgtcaaaa ggata 155
<210> 165
<211> 1624
<212> DNA
<213> 玉米根虫
<400> 165
ggggagtagt acgtagtacg gccggttcta aggtaggcgg ttcgttgtgg tcgtgtggta 60
gtatgccaac cggttggaga aaaatctgtt tttcaccctt tagttctcaa tagaatgaaa 120
tcgtgagttc tgtgacaaat ttcaacaaaa atcgtttata aaaccatcaa agtggcggcc 180
aatacaggat ctcgcacctc caaggataat atgctcaaat ttctcaccca caaattaaga 240
actcattcta taaatgagga taactatgca gggaaggccg atgaagacca tgattctggt 300
acggaatcag atgaagagat tgatgctcca gtatcaggat cagcatcctg ttcagaacgc 360
gtttgtaacg tatcaccggc agacacagga tgttcgatgg aaagtccagc tcctgatcaa 420
cacgatgctc acagctccga agaagagttg gaagttatca atagcaatag taaaatgagg 480
cgatcaacgg cacctcccag gccgctgagg gctgcgtcag tttgtctagc tgaaaaacga 540
aagtggtctc aaattggaga cgcgcagaat cgaaggtata cacccgaaaa cgaagagaac 600
atgtcccact atagtaatct atctccgtct ccttgttcgc cgttccatat aaaatgttgt 660
agcgaagatg gtggacacca tattgaacat tccggctcgt cggatgatga ggtgcaccac 720
ttcttgtaca cacgggctac tcctgtacag ttccgcactt ctcctccttt agaagcggta 780
aaacctgaac gaagatcaat tgtaacgagc aagggtcggt cgacgagccc gcctgccaaa 840
ctacttcact acgaagattc cccaagaaaa agaaccaagc acatgaaaca tgcacatatc 900
cagaggccat accttgattt ttgaaaaaat gcaacagatg aaaacgaggt cagtaacggc 960
ctggcgccac accgctgacc acacaggaga gctgtccgtt tactgttggt gagatcagct 1020
taaggggcgg actccgtcgc cctccgactt aagtaatcac cccatggagg gctgcagttc 1080
gtcatcctca tcatggcctg ataatccttc atagtctaca aaataactct aaccgaaact 1140
aaaactacta aagaattgct gcatagaagc actgaattgg tacacgtggt ttttggaccg 1200
acggttttgg atttgggtag ttggcgtaga ctggaatact tcaaatggaa tagacgtgcg 1260
ttgaagatct tgcgtcgaaa gcatttcgca gactatatag ttttgtaatt ttatatggac 1320
tcattaattg caataaatat tttccactca aatttgaagg cagaatacag gaatggtact 1380
aaaacgggta caactcttaa gatttcttaa ttaactcaac aagatgttcc taaatactcg 1440
gctagaaatc aagtgaatat aggtacgctg aatattgttt gaacccaaca gtaatgcccg 1500
gtttcacaaa cagctgttaa ttgccttatt cattatgaaa gttaactgtg acagctatta 1560
ttaaaggtca tataaatata ttgtatgacc aaaattccgc agaattttgt agtcaatcgg 1620
tata 1624
<210> 166
<211> 1348
<212> DNA
<213> 玉米根虫
<400> 166
aaagaataca aagagaatga tagtttgaac atttctgaca atgaaaacat aagagaagga 60
gttactttgg aatggtttaa taagaattta aatgacaccc agaaaaatgc tgtaatgcag 120
gttctttggg gtactgctag acctcttcca tatattattt ttggacctcc aggtactgga 180
aaaacaatta ctcttgttga aacaatttta cagttaacac gattaattcc ttcatctaga 240
ttattagtaa ctgcaccatc caatagtgca gcaaatttaa tagctctgag acttattgat 300
tcaggagtac ttttaccagg agatcttgtt agattaattt catataatta tgctttaaaa 360
gaaactattc ctgataagtt attaccctat tgtgccactg caggcttggc tagagaagat 420
actgttgttt cagaagtaaa taacccaaat ggaatacaat ttggtagcaa tatgtctact 480
ttaggccgaa gtagaataac tgttgcaacc tgtagttgtg caggaacgtt tttccagttg 540
agcattccca gaggtcattt tactcatata gttgtgacga agcagcacaa gctggagaac 600
ctcatgttat gattccttta tcttttgtaa ataaaaattc tggccaaatt atattagcag 660
gtgatcctat gcagctaggg cctgttatat tatctaaagt tgctaccgaa tgtggactag 720
cagagtctta tttggaacga attatgagca gatttccata ttgtcgtgat ccagaaggat 780
ttccagaaac gtctggattt gatccaagat ttgtaacgaa attattatat aattacagag 840
cccttccgga cattcttact ttgtacagta cactctttta tcataatgag ttgataccga 900
ctattgacga tgaaactagc aatgaagcaa aactgctaac ttctcttcaa gatttcttac 960
cagtcaaacg tgataagcta acccgaatac tatttcatgg agtaattggt gaaaactatc 1020
aaacagcaga ttctccatct tggtataatc cccaggaagt agcccaagtg ttttattatg 1080
ttaatcagtt ttacaggctt ggagtaaaat ctggtggtat aggtattgtc accccttata 1140
ttaaacaggc taaagaatta cgaacagttt tcacggaagc agaatttgaa gtaccaaaaa 1200
tcgggactat agaagaattt caaggtcaag aattcgacat cgtcattata tccacggtac 1260
gttcatctaa agattttatc aaatcggatc ttacttacca cttgggcttc gttgcacacc 1320
ccaaaagatt gaacgttgcg atttcgag 1348
<210> 167
<211> 6699
<212> DNA
<213> 玉米根虫
<400> 167
gttagattct ttgacaggtc taattgatta tcgagtgctt tctttgaaaa gctcgacatg 60
gagtcccaaa acatgaccat gatgcactcg gccggaccga ttcagccccc accaagggaa 120
ggggcggccc ccatcccaac agaaggggta accgcaaggg gcacggacca cttcacggaa 180
atctaccgac agagaggtta tccttggggg aagaagccaa attgctctac tctcaagaaa 240
tggcctccat gccgagcaag actcccgtca gcgtcctcca ggagttgctg agccgtcgcg 300
gcatcactcc caaatacgaa ctggtccaaa tcgagggcgc catccacgag ccaatcttcc 360
gctaccgcgt gttccttaac aacgatctgg tggccaccgg aaccggaaga tcgaagaaag 420
acgccaaaca ttcggcagcc aagaacttgc tggatctctt ggtcggaaaa gtgactcccg 480
aacaagccaa tcagaccaac ggaacgcccg gagcggctga tatcaccaat caggtggtga 540
catcgttcga tgacaaagtg atgggcaatc ccattggctg gctccaagag atgtgcatgt 600
cccgcagatg gccaccacca ttctatgaaa tggaacacga agagggacta ccacacgaaa 660
ggcagttcac catcgcttgt catgtgttga aattccgcga aattggcact ggcaagtcaa 720
agaagctagc caagagaatg gcggctcaca agatgtggca atccttgcag gatttacccg 780
tggagggtaa caatctgcct tctacatttg cgagtgacga agagactctc agcttcaacg 840
ccaaggactt caactttgtt cagttcttgc aggagatcgc tagcgaacaa tccttcgagg 900
taacctacgt ggacatcgaa gaaaaaacat tgaccaatcg ccaccaatgc ttggtgcagc 960
tatccacttt gccagtggcc gtctgctacg gaacaggcaa aactccgaag gaagccaagg 1020
gctcggctgc tcatcatgcc ctcgagtacc tcaaaatcat gaccaaaaag tgaacagcta 1080
tcgatacaca gtcatagatc caatcctata attatccatt tcctgtaatt ctggtttctc 1140
tatagttttg tcatacgttt ttgaggcagc aaatttctca agggcacacg gcaacaaaaa 1200
gtgataatgg atacaaattt gactactgta tagctttctt ctcttggtga acacatactt 1260
tattcgtcat cgctccccac gagcgtttct ttagtctgta tattttcttg tatcacttat 1320
tgcgtctttg ctttggtcac tttacgcgcg aaaaccatta tgttttacta atttttcaat 1380
aaagtacact ttatttaaga acaggggaga aaagtcacga gaatttgaaa agcgagcttc 1440
atgatcccct gaagaatata tcttgaagaa ctattatttt ttaatttttc atcacagtga 1500
tgtttgtttc agaacaaacc gggaaaagta ttatgagaat atgaaattta aaagaaaatt 1560
tgaaaagtat catgagtttt gtgattctcc gaagagtatg tctcgaaaga cttcgacttt 1620
ttactatttt tgtatgcaca aaattcgttc tacatatgaa acatgtcagt tatatattca 1680
cctttatatg tcacattttg tggttcttta acgaaatgtc aagagcataa agtatgtcta 1740
gaacgatttt acctttgcgc gaaaagtgac cacaagcaat gtcaaagaga aggatagcaa 1800
ctaattttac aagctatact ttgcatagaa ttagtggttt ttttctaaaa tcaatttaca 1860
tattattttt gacttgaata aatttttaaa tttaaaaatg cgtgtgtgca ggtatattta 1920
tcacatcatt cgtcgtgtac cacatctcgg atcttgctac taatattcga ttcgataggt 1980
gctttgtctg gttaacttta gtgggaaggt ttaggtattt gtggtatctg tcgatgttgc 2040
cagaacgtaa gatatcctaa actcactttt ttccgaaaaa atatcgcaat agctcaaaag 2100
ggaacaattt ttgtcgagtt attgatttcg aggaataata cgaacttcca tagattcaaa 2160
tgaagtaaac aacatacgtt tttcatttaa aatgaactca catatgcaat gcattcaaca 2220
ttccatagat tcagtgaaaa cacgccatcg ggttctattt tgcgcttagg ccttcacctc 2280
gtttcaaggc tatccttaac atcttatctc gtctatgata tatcttctcc aagtttgtgc 2340
tgggcaaatc aatcaatttg cctcgaaatc taaggttata ttacttttat gaatcgtgat 2400
aacatgcata aaatataata aaaatttata gttttttgca tttttattcg gcttattgta 2460
atattcctcc atagttctta catccttgtt tatttccctt tttgtggaga ggtactatgt 2520
gtgtttggct agattctgtt tgacattcga aaagtaaacg tcagtttgcg tttcaatgca 2580
tcgataagac gaatggtaaa gctgctaaaa aagcaggaaa agcccagaag aacgttcgac 2640
tttcacttca ttctatcggc ctttcttctc tttgccgtat tttattttgt aaggtgaggt 2700
atttgtacat gaagttgtct gcccctttct taatcggttc tgcatgaatg ccatctgggc 2760
ccggggctct gtgatttctt agtgtcaata gtgctgccgt tacttcctca agcgctggta 2820
acgctttatc gggttctgct aagtggtaag tagaaggtaa gccacttaag cacacatgtt 2880
acataaatgt caaaatttcg caaatatata tcacataatt tgtttgtgga cataaaggaa 2940
agaaaaaaat gtcaaaattt tgcaaattat gtggcatttt gacatttatg tgacataatt 3000
tgcgacattt gacgtacggt tcaagtagct gaagtaaaag tgtgctccat tggtttatcc 3060
tctactgtac aactgtttac ctgtgtatga taaataaact tttttcgggt ttggaaaaat 3120
ttcaaattgt tcaggtaaaa tcttgcttca tgattgaaaa tttgcaaggt ttggaggagt 3180
tgtactaatt tgtgtaattg aagttcgtat tatttcctcg aaatcaataa tgcaatgtgt 3240
ttaaattttt gtcgagtcaa aagcgaagaa tatttgaaaa tttctaatat tgcgatattt 3300
ttttatcgat ctggcttcaa tgtttgaatg atgattatat aacgagtaga tagctttaga 3360
ggtaatacac tattattagg cactagttat taattataat catcaatatt atctatcgat 3420
actgaatgta attttttata tagtgccccg atgatatgat atgtatttat tatattgtga 3480
aaagggagca tttatttgat gcaaagtata gaggttgtac ccaaactttt cggggtgcaa 3540
tcttcttttc cggaaaatgc tcgaaaactc aattggcgca cattcagcag gttttttaac 3600
agtttgaaaa ccttgataaa tggaacgaaa ttaaacacgc tgagaaagct atggttgagt 3660
gaaaaaggtg ggaaatgttg actgttgtag ctgattaagg ggtgtattct atatttcggt 3720
taaatttaag aggcttttaa aatttaacgt ttagcagtct tctttttaga ataacattga 3780
cattctataa agacgttaga gaggttcact aaaaaatatt aggaatccac tacaaggttt 3840
tcaatagggc attctaacat gtcaaaatta aatcaatatg gcggctgacg ggcaaacaaa 3900
tgttatttgt cagttcttaa tatggtttta gattcgtttt tagttgcctt tctttgtaat 3960
tttgttttgt acaatgatta atttaacatt ttcagtggaa gaattaattt aaaaagataa 4020
tattcctcat ttctgttgtg ttttgaggtg tggaatgcga agtaattgtg atacaagttc 4080
agttttatca gttaccatga gtgcaaattt tgagtgaaat gagagaagaa cagtggttaa 4140
atgctataaa aggtcctata aaaatgctaa ataactacct gtgccatgct agtagtctac 4200
caacaacttt caaaaatatt cactcatgtt catttattca ccggttaaaa ctaaataaag 4260
tcgttcaagt aattctacaa ctaaaaactt cacaaaactg acaacaaaat gttcggaaaa 4320
tacaactaga aaactattaa aggtaaaaaa caaaatgtgg tacaaatata aacagagaca 4380
agttttgtat ttccgaccat attggtgtca ttaacgtcaa gtctcgaaat gccctgttag 4440
atacagcaaa ttgatcgtgt ttaaactact tgtaatgtag aagttgtcca aaaactgtca 4500
gaggccaaaa cacttaaaaa taagaaattg acatgaagtg aagacttctt ttttagctaa 4560
atctattgta agaaatcctt tcgcaaaaaa cgatcgcaga aacttttcca tccacatcgg 4620
ggcggatatc agagaataat aacttaaata gtagtagatt ttgcgttgtg gtgaaaaaaa 4680
aatagagatt ggaacacatt aaaaatattc ataaatctat tagagctaga aattttaagt 4740
gtaacacatg tggtaatttt ttaaaggaaa tcactctgga gcatcctagc gtgtgttcta 4800
cgatagttca tcatagtact ctcttttttt tgtaactcat tttcccatta accattttta 4860
tttatcaaac tatttaatca taaagttacg attttaaggg atatagaata caaaaatatt 4920
ttaacagacg gtaaaatttt agaggcctct taaatttaac gaaagtgtta cagaatacac 4980
ccctaaggca agattaaaaa acggcaacga aggaagagga aattgctgga aattttagta 5040
attctcacat tgtttccgaa aactcaggaa attatctgaa aaactgcaac cgatatttca 5100
taatgttgcc agatttctgc aaatgattgt attttacctt ccagtattcg cgtagatttt 5160
caggagaagc atagttgcat cgagagaccg atattaattg aacgttagtt tttgaaaaaa 5220
aaaagcccca acaacttggg cttttaggat gaataatacg tacatattct tcaagaaaca 5280
gaaattttaa tcagataaac taaatgtggt attactgtta atgcagagaa ggttcctatc 5340
ttcaatgaga tgatggcgac cacattgacc catcttattc tattcacatc agtcttgcct 5400
attcacaatc ttgccttgta gaatcaactg tatgaaaatt aaaaatctaa tttttatatt 5460
gaaatggtgt agaagacaat gcttaaaaat tgagaaaatt gtcattacaa acaacggtat 5520
ttgaagatgg atcgtatgaa gacgaggata ccgaatacac agtaaatatg tgtaaataag 5580
atcacgagaa ctagacgaaa aacacagaaa ggaacttagt gaaaacaata gttgatcaga 5640
accactaaat gttagtatgg aaaacaggga agaagatgaa agtgaaatga ctgaagatca 5700
accagtagga aatggcatta ataatacatg tatatcttga gcgcagaaac gacgaaataa 5760
aaaggaactc gaagcaaagg aaaggaaaaa tctaatttta gagcaagcta agaagaataa 5820
agagggaccc agaattgtta aaatccttaa aatcagctgt agacgcagta ctaccataca 5880
acgtgacagt gtgacaaaca aaatttcgac caatcaagtg ccaaatttca tacaattttc 5940
gatacaagaa cttgcatatt gttatacagg gcacaaatgt acgtacaaga aacgatacaa 6000
gaaaatgtat atcagaaatg atattagaaa tgatacaaga aaatgcatag tgtcatacag 6060
ccataagttg gtaaatgatt taagaaaagt tacagcagat tttgtaagac aaaacaaaga 6120
tgacttcaaa ccatttatgt gcaatgaatt aggtgaatcc gaagtagtta gcgaggaaca 6180
gtttgagaat tattgtaaag atgttgcaac aacaaattaa tggggaggtc agttagaact 6240
gcgagcttta tcgaatattc ttacttactc cattgaagtt attgaggccg cgattccttc 6300
cacactgtag gaaaaaattg cagtttgctc actttcatca atatcaaaaa ttgaactttg 6360
acccggtctg ccagaccgtg cgcgactact ggaaggttta aaataccttt ttatcgcata 6420
ttccccataa aagtgaaatc tggcagtaca gtgttgcgaa attgagggct ttttctggaa 6480
aataagatta tgctcttttt gatttttgtg aacggtgatt gtggaagagc aaacacctcg 6540
actggttctt tgggtataag cgacggtgtt tcctcgtcgg caatcgctgg gttctaatta 6600
acatatatgt caatagaaag tttttaggca caaaccgtca atacatatag gcatgctatt 6660
gtcgaagatg tctaaagatc cctagaactg tagaattgt 6699
<210> 168
<211> 1463
<212> DNA
<213> 玉米根虫
<400> 168
cctccgaaca gcattatcaa aaaatgaatc caccgaaaat ttagacatac aattagcggg 60
atcttcgaaa aaagccttgc tgtccaatat cgagttcgaa tcggctggga gctatcataa 120
tactgtctta gataggctaa aatcgaaata tatagtgctc agaagtcctg aatcaccacc 180
cccaagtaaa cctgaagatg acatgaactc cagtgaatcc ttaaaattag caaaatatga 240
actatttcca ctgagcgatg tacaactcgg ttggaataac tgtgattggt ctgttggtgc 300
tggaatggtt aatatgggaa atacatgtta tttaaactct actctgcaag ctctctttca 360
tgtaccagct tttgttaatt ggttgatgtc agacaaagat catactgcca tctgtcaaga 420
ttcaggaggt ctctgtataa tctgcgcgat gcgcaaaact ctccaagact cacaacaacg 480
taacgccaac tcgatccgtc cattgttgat ctacaacaaa ttgagattgg tgtgtcgaaa 540
cctcataccc ggtcgacagg aagacgccca cgagttcttg agatatctag tcgaagcgat 600
ggagaaagca ttcttgagcc ggttcaacaa ttataccgaa ttcgactcga aagtcaaaga 660
aaccacccct ctgaatcaaa ttctcggggg gtacctgagg tcggcggtta ggtgcttgaa 720
gtgtggccat gtcagtacga cgtttcagca ttttcaggat ttgttgttag atataagaaa 780
ggcccaaact ttggacgaag ctttagaatt gtacttttct agagaaaagc ttgacgacga 840
atcttatcag tgcgaatctt gtcagaaaaa ggtaccggcc acaaagcagt tttcgataga 900
acggacgccc atggtgcttt gcatccaact taaaagattt tcagttagta acaataaaat 960
aacgaaacac atcaacttta gacaaagact ggatctaacg aagtacgcta gacatcgccc 1020
taacgtacct ctgatctaca ggctcgtagc gttggtcacg cacatgggcc ctaccgtcag 1080
ctgcgggcac tacacggccg tcgcgcaagc accttcaggg aatttctttc aatttgatga 1140
cagtatggtt agaccaatat cccaccaagc agtgttcaac accaacgcat acattatgct 1200
atatgagttg gaatcctcgc cctactcacc taaatctgcc cccgccacgg taactagcaa 1260
actgaagact tcaaccccaa ctgccgaaac cagttcagtg gcttgttcct cgaattccag 1320
caccgtaaat tcggtgaccg cctccacaaa gtcgtacgcc aacggcattg ggtttactag 1380
cgggaaggtt tatggtccag aactgcctcc ggataggata gagaatagtc ggaatggata 1440
tgtgacaagc agtaatggta aag 1463
<210> 169
<211> 2813
<212> DNA
<213> 玉米根虫
<400> 169
aacagcaacc tggagcgaga caacagcagc atcaaactgt tcccccacaa cagactggtc 60
ccccacaaca gactggtccc acacaacaga ctggtcccac acaacagact ggtcccacac 120
aagggcagca aaaaggtgcc caacaacagc aaacgggtcg aaatgaacgt caacaacaaa 180
gttccccacc aaagcaaatg ggtccacatg gagatgtacc acaacaacct aaatcggcaa 240
acgggcaaca aaggggtgga ccacaacaac agcgtaggcc tcaggatagg cagcagacag 300
cttctgttaa agaattgaca tctcgtatga gtgaactgca agctggtcca cttgtaccta 360
tgaggttaag aaaccctgaa cctggaaaag caggtcgcaa aatacccgta gaaacgaatc 420
atctcagcct cgcgctaggt aaattaaaca cggcttatca ctatgatgtt gatttggtac 480
cagatacccc gaaaaagttt ttaagaaccg tgatggaact ctttaggcag caacattacc 540
caaaaagata tccagccttt gatggaagaa aaaatttata cagcactact atgttacctt 600
ttggagaaca aaaagaaggc gaggttacta ttcacgacag tcaaaataga gagaaaatat 660
ataaagtgaa agtcaaattt gcaaattcgg ttgatttatc ccccttgaaa gattacgatt 720
tatctagagc aactccgacg gaagctatac aagtggtcga tattgtttta cgttgtgctc 780
cttcacaaaa tctacttcaa gtgggcagaa gttttttcca taagccagtc ggagaaatta 840
tcgacttagg agaaggcatg gaaatgtatc acggatttta tcaatcggcc attcgaggct 900
ggaagccact tttaaatgtt gatgttgctc ataaaccgtt tcctaaagct ataccagtta 960
tagaagcttt gcttgaagta ataaatgcag acagatataa taataaactt acgagaaatg 1020
accttagcag acctcttgat aggcgagatt tggaagtttt cggcaagtac atgaagcagt 1080
taagagttgt ttatgaaatt ccaaatttgc ccagttctag aagaagttat aaggttaacg 1140
gtataaatga cccaccggcc gtgaaaacct ttaaagatgc gaataataga gaaattacca 1200
tccaacggta ttttgaaaca gaaaaaaggt gcaaattaag atatcctcag atgccgacac 1260
tatgggtcgg atcttcagct agatcggata atcctatact tgtacctatt gaattgtgta 1320
caatagaaga taatcagaca attaatagaa aaatgacaga aggacagaca agaaacatga 1380
taagatatgc tgcaacctct actacagtac gcaagaataa gattatggaa ggaataacac 1440
gggctaattt taacaaccat cccactgtac gagaattcgg tttttcagtt tctagcgcat 1500
ttgaaaaatt agatgccaga atcttgcccc ctcccagatt aggctatgcc ggtaaagaag 1560
tgaatgttga taaaggaata tggagaggtg acaaattctt ccaagcggtc acgattaaca 1620
aatggacaat cgtgtgcgct gataggaggc caccaagacc agatgactta cggaacttag 1680
cttcacaatt acttagagaa gcacgcggaa gcggtatgca gattggtgaa gcagaacaac 1740
cattctgtac aattggtgac agaaatatgg acatcaagaa atacttcaca tctgttaaag 1800
gcaaatatga tgtaatcttt gtcgtcgtac caaatagcgg accacaatat agttacgtta 1860
aaacagcagc tgagattaat gtaggctgtt taacacaatg tgttaaagta agaactgtgt 1920
taaaaatgaa ttctcaaacg gcgttaaacc ttttacttaa ggttaatgcc aagttaaatg 1980
gtactaatca ttttctatct acccgtccgc ccattttgaa tagaccaacc atgatcatgg 2040
gcgctgacgt cactcatccc agccctgatt cgcaacatat accgagtgtg gcggcagtca 2100
ctgcatctta tgatccaaaa gcctttaagt acaatatctg ctggagattg caaccaccca 2160
gacaggagat catcgaagat ttggagaata ttgtggtaga ccaattgaag ttcttttatg 2220
agtctaataa aggtcaaaaa ccccaaagaa ttatcttttt tagagatgga gtatcggatg 2280
gacaatttga acaagttaca agtgccgaag tacgagctat tcgagcagca tgcaagagag 2340
tccaaagaga aggttatgaa ccggcaataa ctttccttgt tgtccagaaa cgtcatcata 2400
ctagactttt cccattgaat ccaagagatt ctcatgacag aaatttaaac gttccagcag 2460
gtacttgcgt ggacacgcat attacacatc catttatgca ggacttctat ctagtttccc 2520
acgccagtat acagggagtg gcgaaaccaa caaagtattg taccttgtgg gatgacaacg 2580
atatgtcaaa cgacgacatt gagcaactta cgtattttct atgtcacatg ttcactcggt 2640
gcaacagatc cgtaagctat ccagctccga cgtactatgc ccatttagct gcggccagag 2700
gcaaagtcta catagaaccc gaaaatgtgg atttacaaaa tttaaatagg gaatatgaaa 2760
gatttaaaat ccaggacagt atccagaagg gactgcctat gttctttgtc taa 2813
<210> 170
<211> 4075
<212> DNA
<213> 玉米根虫
<400> 170
caaaacaatc ggagctgagc tacgcctaga gtcatggttc aaaacgagta ctacgaaaag 60
gccagcgggg caaaggccca cttccacggg gcaggcagca cgtcccaatt cggagataga 120
tacaggagac gctgacgatg aagacctaaa acttatcgac gtcatgagcg agaaggaagt 180
taatgctaag atcgaggagt tattgagtga catgaacctc aacgacgaca aaaagaaacc 240
ccttagagaa atgcccatgg aacagaaacg tgcaatgttg aagatgcaga acaaaaagaa 300
aggacaacac gacacgggca gtcgattcaa cgatcccgaa gattattaca gatatctgga 360
tcaacacata aatccggagt acttgcagtt gaacaaatgc ctgaactgtg tggaaagctt 420
gagggtagcc ttgactaaca acccagtcag ttgggtagag aagtttggga ctataggagg 480
tttacagaaa cttgaacaag tattggatac gggaataagg aataaagatg ttcgacttca 540
aacagaatgt ttacgatgtt tagaaaaatt catgaacaat acgacgggac ttaaagcttt 600
tttcaaattt tcaaaagcgc atttgactgt agccagatgc ttggaccacg aaaagcctac 660
tgtgatgtta caagccttaa agattcttgc acctctatgt atcctgaact tggaaaagag 720
cggcgaggaa gaaggtgtaa aaaaggtgct tagagccatt accgaggtgg gagagataca 780
ttcgagagaa cggttcctat ctgtggtact tggaatcact gccagcgata atgccgaatt 840
acagagtatg tgtttccaat tcatcaatgc cttattaagc gaaacggagg acttcgagtt 900
ccgtatgcat ttaagaaacg aaattgtaag aaacggactt tacgaaaaac tacaagaact 960
aaggaacgag agtaatcccc aactaaaaac tcagttcgaa atattcgaaa acagacgaga 1020
agatgacgcg gacgaatggc acgaacgatt tgacaaggtc aggctcgaca tggacgatat 1080
tcaagattgc ttcgaggtct taaaaaatat cactctagat acgccgtcgg agccttattt 1140
tttgagtatc ttgcaacatc tcttgttcat taaggaagat gtcaatatca ggccagctta 1200
ttttaaatta attgaagaag tgataacgca gatagtactc aataaggcgg ggctagatcc 1260
tgattttaag aaaaaacata tagacatcga ccttcagccg cttctagagg aacttaaaga 1320
caaaccaatg caaattgaaa gtgcagaagt cgagagccta aaaaaacaac tcgaggaagc 1380
aatcgcagca aaaacagagg cagaagccaa actagaactc gtccaaggca gagcgggtac 1440
cacacagcct tccggaaaac tagatccagc tttagtaaac aaaatcaatg tgccaccacc 1500
tcccccaatg ccaggcgccg gtgcgcctcc accacctcct atgccaggaa tgggaggcgg 1560
tcctccccca cctccgatgc ccggaatggg tggcggtcct cctccacctc cgatgcctgg 1620
aatgggaggc ggtccacctc ctcctccaat gcctggtatg ggtggtgggc caccaccacc 1680
tcctatgatg cctggaatgg gcgggggacc gccgccgcca cccatgatgg gtggtatgcc 1740
tccacctcca cccggcatgg ttcccttacc gggtttggcc aggccggatt tgttaccaca 1800
cggtttaaaa ccaaagaaga agtgggaagt tacgggacca ttaaagaggg ccaattggaa 1860
aacgattcta ccacaaaaaa tgtccgaaaa ggccttctgg gtaagggcaa aagaggaaga 1920
ccttgccgaa ccagatattc tcgacggttt ggctaagaag ttctcgtcta aacctgccaa 1980
attaccagaa gatgtgacag ataagagcaa taatacggga acgatgaaga aagttaagga 2040
acttaaagtg ttggacggaa aaactgccca aaatattagt atattattag gtggatcgct 2100
caaacacata ccatatgaag atattaaaaa cgctctactc agatgtgacg aagctattct 2160
atctgataat gttactgaac aacttattca atacttaccc cctgccgatc aattaaacaa 2220
attccaaaac ttcaaagagc aatacaaaga cctgaccgag gctgaacaat tctgcgtgaa 2280
gatgtccgaa gtgaagaggc tgttgcctag gttgaaatct ctcagcttta agcatcatta 2340
cgggaaaagg gagcaggata tcaaaccggg aattgttgct gctacagctg cttgcgacga 2400
agtgaagaag agtaaaaagt tcgccagaat tttagaacta attttattga tgggtaacta 2460
tatgaatacg ggaagtaaaa atgcgcaagc gttcggattt gaaatgtctt tccttaccaa 2520
gttaacctcg acaaaagatg tttcgaataa gcaaacgctt cttcattaca ttactgaaac 2580
gatagagaac aaatttccag atctgcttaa tttttacgat gaaatgcctc atatagatca 2640
agccagccga gtatcgttag atacgataca gaaagcgtta caacaaatgg acactagcat 2700
aagaaattta aagacggatt tgacaaataa cagggtacct cagagtgaag aagacaaatt 2760
tttagaagtt atggagaaat tcgccgaaga agccagagaa caatgcgaca tcatgcaaaa 2820
aatgttaaag aaagtagaaa acctctacag cgatctagct gaatattacg tgtttgacaa 2880
acagaaatac gcattagaag aattctttgt ggatttgaaa acgttcaaaa atagtttcct 2940
gcaagccaaa gccgataatc agaaagaaaa ggaaatcgaa gagaagaaag agaaggcgag 3000
gttagcgaaa ctaaaacaag aaaaagaaaa ggaagagaga aacaaacgaa ggctgattga 3060
tatgaatcct tcagaaacgc aagaaggagt gatggacagt ttgctagagg ctctttctac 3120
gggtagcgca tttggaaggg agcagaaaaa gcgaagaggc catcgaccag caggagctga 3180
aaggagggcc cagttagtta gatctaggtc gagaacggcc ttaatagctg gaagagaatt 3240
aactagtgaa atattggcgt agcattaagt aataataaag aagaacgcac aacgacttga 3300
acgctcaaaa gaaatatatt atataaagaa atattttata cgttgtaata atttattttg 3360
taaattgttt ttccatttgt aaataataat ccgtatatgt atatttagaa attgaacgaa 3420
tgaatctctt atatatagtt ttagttttat cgcagattat tatgtaaata tgataaaaaa 3480
ataaaaaaaa tcagtctttt gttaaacttt atattgccgt aaaagtagat tagtttttta 3540
tatattgttt ttgaaattga acaaattctt gtacgttttg aggtagtgtg cttttataat 3600
atgtaaaatg ttgtaattat tatatacttt actgttcgaa tttttgttag gttttgtatt 3660
ttagttttat ttatttcttt aaaaaaaaaa tgtgtatggt taattaacca aatatatgcg 3720
tactgttttg tttataagtc attttaagtt aagttcaaaa agctctttaa ttggacctct 3780
gttcttgcta ttttttgtag tgggtgacat caaatacaag atggcagata agtttatttc 3840
agaacagaat tttcattcct aaaaaatcct ataaaaccaa ttaaaagatc tgttgcgctg 3900
ctatgatgtt cttcctttca ctgcactaac agaaagagta gtaccctgta ccagtatctt 3960
cctggatcat tcactctccc tggaccactt tttgttacaa aaagaatagt actagggagt 4020
catcattatt tacgtttgtc gctatacagg atgttagtga ataagtgtga gaaac 4075
<210> 171
<211> 2294
<212> DNA
<213> 玉米根虫
<400> 171
cttctaccat tgatagtgct atttataaat aaaaatattt tgtgtttatt agaaaaaaac 60
tgttttatat taaaaaagag aaactgtata gtagtgataa agtgtatggt gcaaaatgct 120
gttttctaat gagatctggg cggctacaaa acggcaggca ttgaaaagga cgaattcctg 180
ccaggataga attgaaagga tccctcctag ctcggaagaa cttttcgaaa atatcccttg 240
gatgaatatc atggctgatc tgactgttaa atctgctata gctgaatatg agtttaaacg 300
tatgctgaat aaggagctgt ctcatttctc cgaatctagc aaatctggca atcaaatatc 360
ggaatatatc tgctccactt tcctagacaa acaacaagaa ttggacctac cctcccttcg 420
tgatgaagtc cctgagctcc tcccaaaacc cactcaacgc aaagaacgtg cccgaggacc 480
gcattctacc atgtctcaaa tatcaggtgt taatcgtaaa ccactttgcc acaccaattc 540
ttttaccggt gagaagttac ctttgcacgg cattgaaaca ccttacgaag aagaattagg 600
caaaagtttg attatgatag atcaatgggg tatcgatatt ttccgcatcg gagagttaag 660
caacggaaag cccctaacct gtgtagctta cacgaccttt aacaatagag acctcttgaa 720
gacaatgaac atcccgccga agacgtttat aacttttatg atgaccctag aagaccatta 780
tgtgaaagaa aatcctttcc acaattccct gcatgcagcg gatgtggctc aaagtacaca 840
tgtactgctg aatactcctg cattagaatc tgtatttaca ccgctagaag tgacagcagc 900
tctattcgca gcgtgcattc acgacgtaga ccatcctgga cttaccaatc aatttctaat 960
taattctagt tctgagttag cccttatgta taatgacgaa agcgtactag agaatcatca 1020
cttagctgtt gcctttaaat tactttctaa cgacggttgc gatattttct gtaatatgac 1080
caaaaagcag agacaaactc ttaggaagat ggtcatagac atggttctta gtactgatat 1140
gtccaaacat atgactcttt tagctgatct aaagactatg gtagaaacga aaaaagtagc 1200
tggttcaggg gtacttcttt tagataatta cacagataga atacaagttt tggaaaattt 1260
agtgcactgt gcagatttaa gtaatcctac caaacctttg gatctctata gacgttgggt 1320
agatctcctc atggaagaaa cttgggcaga tctagtgcat cctgacgctg cagcaaggag 1380
acaaagaaag agaatccaaa atggatatca gtcccatgtg cgataggcat tcagctacaa 1440
tagaaaagac ccaagttggt ttcatagatt atatagtaca tcccttatgg gaaacttggg 1500
cagatctagt gcatcctgac gctcaggata ttttggacac cttagaagaa aaccgcgatt 1560
ggtatcaaaa cgctatacca ccgagccccc ctccagaaga gactcccgag tcccagcgac 1620
caggcatcag attccaagtc actttagaag aaggcgaagg ggaatctgaa gaaggtccga 1680
tgtgacggaa actagggggc ctctgtagaa agctcactga aaaggtgcag caatggtggc 1740
gtatcaggca gtaagcgcaa gtgcaggcgc atgcgcactg actgacgaac gttacaccgc 1800
gcaggcgcgg gtttgttgtg ttttgcaata tatgcttgac tgaaattttg aatttttcgg 1860
gctgagatgc cagcctgttc ttatatatcg cagagttcct gggagtgtac tcttttttca 1920
attttttgtt cggtttttta gcaaatctgg tagatacatc gcttcatggc caaatcgtaa 1980
gcttaaataa tctaggaaat acttttcaac agattcataa ccatccctaa acataaacta 2040
aacagttttt aagaaaaaat agtaatttgt aaatctttta tgtttatgaa ttttgaaacc 2100
gtattgttaa atgtaaacca tgtaaaagta ttgttaattt caaagtgact gtaaacagac 2160
ttaaaatagt aagtcgattt acagtgtgac aatacaaata taggcatgtc aatgtagctt 2220
tttatttata tgaaatagcg gtataaatct tcaaaacatt aagataacta cagtaggaaa 2280
aatgaaagaa tacc 2294
<210> 172
<211> 3145
<212> DNA
<213> 玉米根虫
<400> 172
ttttgataca caatatctct tgcgaattat atcagaaatt ttacaaagct acttttttcg 60
attttacttt aaattaggag gtaaaaagta attgtctttc attatatcaa aaagacatct 120
gagcattatg cattacaata tgacagcatt tttggggaaa aaatatgaac gtttttgttt 180
tctgaaaaac cgtcatattt ttttccaaaa aatatttgat tggtgtaata gcctaaatat 240
atttgaaaac gctaaatact ggcgaaaaat actaaataca aactaaatga taatttataa 300
tttatcacaa aaattccaaa atggtcttat ttaagtgtcc agtaaactag tgctgctaat 360
ttggagatag gactagctca caccaatcat agcttaaata aaaaaggaaa gcagacttta 420
acacctcatt attttaggtt ataaaacaaa ataagtattt gaaatttatg tcatagttat 480
aaaaaaacat ggtatagttt tttactacaa aagtcaggtt aagtcatagt ctatacaaac 540
tgacgtcact tgtagttctt caatgacagt ttatacaaac tgatgtcaat tatagttctt 600
caatgacagt ggggcgattg gaaaaaacgt ttacagcatt gccaacaatt tatagtaaga 660
attgtgtttg ttttgatggc atctacacca acgaccaacc aatgcaccgc cagactgaga 720
gttttgttta cgctgacgac ctcggtatcg ccgtaaaaga gaaaacacat acacaagtaa 780
agtcgacaat ggaagaggct ttaaacgtga tgtcaactta ctacaaacaa aactcattaa 840
agctaaaccc tactaaaacc caagtatgtg cattccatct caacaaccat ttggcgcatg 900
gcaaactgaa tgtatcttgg gagggacaac gcttggaaca tactgatagg cccaaatatc 960
ttggagtgat actagactgg tcactaacat ataaattcca ctgtccgagc acaagacaaa 1020
aggtttctga gagaaaaaga cttctccgga aacttgtagg gagcaagtgg gtgcaaaccc 1080
ccaggtgtta aagtcaatag ctgaggcctt atgtttctca acaggggaat atgcttgtcc 1140
cgcctggggt aaatctatgc acgcccaaca agtcaccacg gcgttaaata aaacatgtag 1200
aataactacc agttgtatga agcctaccct tctaccccta ttgtatcggg cagctggatt 1260
cgcatcacca gatgaccgta gatgcgcctc acaatgtggg gaagttgaat gaaaggcatt 1320
aattatacga gtttgacgac ccgtcaggaa ccagccgact taaatcaggg aaatagttta 1380
tgagaaatgt cagcgtcaaa ctacccgacc tgttccctct acagccagaa cgacctaatg 1440
gaatgaacct ggactggaga aactggcgga cacttaaccg catacgcacc ggttttgtcc 1500
ctctaaaaca gaacctcatt aaatgtggca tcaagacaga taacgacgct ctttgtgaat 1560
gtggggaaat acaaaatttt gtggttctga cgtcagaaaa cttgaatgat gtaagttaat 1620
ttttgtagta aaaacaccat agttggacaa ttactataag agaaaaaata tcacaatgtt 1680
agtaggtttt tccatttgac tttctacaga aaaagcttag taaaatgcaa tcaactaaaa 1740
gttcacttac actgtctcat ttttaacaaa aaaaatgttt tataacctaa agcaaaaggt 1800
tttttttgaa aacttaaaaa gccaataaac aaatttcact ttacactgtt aaaccgtata 1860
aatattttgt gcttactcta accctttttc tgataaattc cgtttttaga tcccacatcg 1920
gcactattac aaaaagcaca ggagcccagg agaaaggaga agggtcgaaa ataaaataga 1980
gacagcagga ctagagattt ttttattaaa aaatcctaaa ctgtaagttc ttcagtatca 2040
aatgttttta gagatagcaa tggtatgaga aaagcttaac aagaaaaatg cgctttatta 2100
taattaaaaa aatcacatca tgttccggaa acttatctca gttaagtttg cgaaacactc 2160
tcaaaaaaaa acttaaaata tcctaaatac tcttaaaaaa caatgataat tgagaccaca 2220
gtcataaatt atgattggca aattggcgca gtcgtgttag gaggtcacgt attgtataaa 2280
aggttttaaa agacaaattt ttttgttata aatttaatat tatgaccctc gtaacataac 2340
tggaaccaca cgatggacaa cgatgaaaaa attaatgatt ttgaccgata tataatatat 2400
attttatgtt gtttgtttgg taaaaaaagg aaaatacaat attgtccgat tctatgactt 2460
ttttccatgt attttttaat gatgatgcac tcggtatacg cattgccggt tcgctgttat 2520
tttgaaaaaa atacaaattt aataataatt aatttttcgc cgcacggcat ggctgcttac 2580
agcggcacac actatctcta gtaaccgcag gtaattaagt aatcacctag cttttctacc 2640
actgacgctg cttgctgagg ttggattgga tcttcataaa gggatactac aacagcttgt 2700
gtcgttttca tgcaatgtac accaactttg cccaatttcg ccctaattac acggtctgtg 2760
ccagagaggt atatgtatct gttaccggct aatgttacgc ctgaggatgt gagaatgtct 2820
tgtttttcga agccctgaac taattttgct agttcttctt ttgatacatc gaaattttct 2880
gatttagccc aaacgtttcc gtcgtggcca gcgattgccg ctttcgtaac gcattttgaa 2940
gccaagagct gtttgtctac gtaatcctgc cagctcatat tgttgtaaat ttaaaaaatc 3000
actgcacaca cggttctact cactaataaa attggtgcaa ttagagcagt tgccgcacga 3060
cgaactgaag ccgaatactt ttgtctacca aggaagattt tgtagtggca gcggaagctc 3120
taaagagaca agtgcgactg ccccc 3145
<210> 173
<211> 1208
<212> DNA
<213> 玉米根虫
<400> 173
aaaaggatgt ttagcacaca attaggaaat tttccatttg ctaatactag gaacagccaa 60
gagacaactt ccaacagttt gaataatcat ataatggatg ccaacaatgg agctggagat 120
agtaatgatg gttccataga taaaaccaaa ctaattatta attacatacc acaatatgcc 180
actgaaggtg aactaactca aatattttca accgttggaa aactggaaga tgtaaaaatt 240
atgagagact ttaagactgg ttacagtttt ggatttggtt ttgttaaata ttccactgaa 300
gaagaagcag ccaaagcaat tgcagttcta aatggatata atttgatgaa taaaagatta 360
aaagtctcct actcacgccc accagggaca ggcatgaaag attcaaatct ctacattaca 420
aatcttccaa aaaacattac tgaacaagaa atagataaca tatttaggaa atttggcgaa 480
attgtacaaa gaacgctgtt aaaagataaa attactggaa tgccaagggg agttgctttt 540
gtaagatttg ctaagggcga agaagcacaa gcggcgatca atggcttgca tggaacaacg 600
ttggcaggtt cgatgctacc tcttagtgta agagtagctg aagatcatgg aagacagaag 660
gcacagtatg tggacgtgtg ggatccgatg ggttttaata gagaatctga agaatcattt 720
tcatttggag acgaaggtcc tgacttagaa gaaattctta ttactgaagt gtcatataga 780
agacccctct gggacttatc aattcactcg acgaaaatgc aaattgacaa tctttgggag 840
cagatctatc gtgtatttaa gaataaatat tcgataggtt ttctaactaa catgtggtct 900
aatcttaaag aaggctatat aagagataag agatcatttc cgtattacta tcaaatgaga 960
tttttggatg agtttgaaaa tgttgctcta tgtggatcta gtcgaaggca tacaggaagt 1020
cctttaggaa aaaaattaaa aatggattac cagccacctt cacctgacgg cgttgatgat 1080
tttttgcaac tgttgggaga aggcttacgt agattgcccg ctggaaacag aactaagtta 1140
cagtttaaat ttgtggaatt acttaaagat gaagaatcgc gtatatcaga ttaacttttt 1200
aatatgtc 1208
<210> 174
<211> 2297
<212> DNA
<213> 玉米根虫
<400> 174
gggacctcta aagcagtacc atgtggataa gacattcgcc gacagaagat acaaagtaca 60
gtcagcgagg acatacttct acctcaatga agcccagtgc gagaagaaca cggaaacatt 120
catcaaatgc ttggaaacca tagcagagtt tcagcaaaca tctcaaggct ctacttatgg 180
caccgggatc gcagctgtta aattcactgc tttgggcaga ccacaactgt tgcttcaact 240
ttctgaagta attatgagag caagacagta catgaccgaa attgttggag gtgaaggtaa 300
cgttttgtgt catcacatga agtatgaaga tttggaggcg aaattcgaaa aaattcaaga 360
taaagaatcc ttacgaaaat tcttgaagca agttacatac gataaacaag gtgttctcca 420
cttattcccc tggtcgggta ttattgacga gaaccaagag ttgagtgctt ccttcagagt 480
tccagactta aaatcaggaa ggatgatcag gctattaaca cagctatcac caaaggagga 540
ggagatgttc agaaatatgg ttaggagatt taataccatt gtgaaagcag cacaggaaat 600
ggatgttagg ataatggttg atgcagaaca aacttacttc caaccagcaa ttacccgaat 660
tactctggaa tatatgagaa agtacaacaa acaaaaagcg atagtattta atacttacca 720
atgttatttg aaaaatgctt ttgatgaagt ttcgactgat ttggaacaag caaaacgtca 780
agacttctat tttggagcta aactcgttcg gggagcatat ctcgaacagg aacgagccag 840
ggcagccgcg atgggctatc ctgacccaac caatccagat tacgaagcta cgtctgagat 900
gtatcataaa actctttcag aatgtttgag gaggataaag gccttcaaac aaaaaggaga 960
agacaagaaa attgctataa tggtagcttc tcacaatgaa gataccgtgc gatttgctat 1020
taaaaaaatg gaggaatttg ggatagaacc caaagataaa gtgatatgtt ttggacaatt 1080
gtttgctatg tgtgattata ttactttccc attaggtcaa tctggatatt catcttataa 1140
atacatcccc tatggacctg taaaagaagt attaccgtat ttatctcgaa gagctcacga 1200
aaataaagga gtattaaaga aaattaagaa agaaaagaaa ttacttgcaa gcgaaatttt 1260
caggcgctta tctagaggtc aattatggta tacacctaaa ggaaattatg ttcccgtata 1320
aacactagat ctcaaacaca gcaaccataa aaccactgtg caggttttac ctagttttgc 1380
gacttaccta cccgttttgt agataactag ttggcagaca aataaaacta acgtcgttac 1440
agaaaaatct gcgtgacaaa ttccaaaaat ttaacatcaa ttggcctaaa tatacataaa 1500
ataagcagtc cctatatttt tacaaacatg taaatataaa gcaaataaag gtcattttta 1560
atactgtctg ataacggaaa gaaaagactg tgtttgataa tattttgaaa tgtcattgcc 1620
ttaaaatata taacgaagga atgttttaag tcatgaattc aaatcaaaac attttactaa 1680
aacagatcat tgataaatga tctgttttta gggtatctta tgattgcata ttgaagcgaa 1740
cctcgtttgt tgatttgctg atttttattt ctaaatcgaa tttatagtta tagaaataaa 1800
aataatttta gtttatatct tattgttaac atatttttta ggattgtgat ttttgtgttt 1860
actgatgatt attacagaga aaggacttaa acgtcgcaca tgtttttaac tgcgttaatt 1920
atttgtttac atgtatattt accgttgtga agtaggtatt tttttatttt gactaatgca 1980
aaaaacacat ttcagtatag atgcgtgttg gtaacattac tgttaattac ttcttaaagt 2040
taaaatttga tttggtttcc agtaacagga cttaaagagg taccaacaag gtatgaaaaa 2100
aagcaaaagt aaattattaa accttttaca tgccactata cgtgtagtcc agtcgggtta 2160
gacgaataac tgacctaacc ttgcatgccg agctgtccca gatttggttt ttctgatctt 2220
tgggggaggc cagtagtggt gtgaatttgg aatctcgact gaatgccgcc gttgcgttgg 2280
ctcttatttt gatttcg 2297
<210> 175
<211> 3120
<212> DNA
<213> 玉米根虫
<400> 175
atgaatgatc cctcaaagaa agtaaaaaat ccgttgttta acatatcggt aactggagga 60
acctttgaag attttactca acatgctata ttattacccg caaaagaaaa acaaccacct 120
catacactaa atatatccca tgaacaaatt ttaaatgata taatctccgg atcttcaaaa 180
tcaagtgaag ttgtttctaa tcaagagata gaaacagttg tggaaaatca acagcatctt 240
cccgaaggaa ttaatcagtc agaatttact tattcacaac aactgccaga tctgtcaact 300
ttgcaacctc acttaagtgc tgccccgcaa cacagttcgc acttgtcttc ggtattttca 360
tctttttcta gtatcttgaa tattggaggt tctaataata atcctaaaga agaagtgata 420
gtatctccac ctgcaggaat agaaagtctg attagccaaa gtaacaacaa ccctgagcca 480
gtgccgcttt tttcggccag tttacctgaa tatagtatcc aacagcctcc tccgcctaca 540
ggtccaacaa atttattcag aagagctggt ctaaaaaaac cggcttatgc acaagtacca 600
ggtttatctt cagcccccat agcaccccaa aatttgaatg tccaaaatat tcctccacca 660
aaccttcccc cattacctcc agcagcgcca gtaccatcag cagcatcaca aaacatatta 720
gtaccaacag ccccttctcc atccacttcc atttcatcaa cttctagtgt agaccatata 780
aaccaaggat taccaaatca gtttaaacca atatctccgc ctccttctaa tttgcaatcc 840
ttccatccta tcacccaacc tgtagctcct gtgccggtac aaccttttgc ccaatctgta 900
gcgataacgc atccatacag aaatatagta ggcccagtta tggatgccat accagctagc 960
gaactatcac aaaatgctaa tttcagtagt tccctcgtga attctccaga gtctctgcca 1020
aaaaataata acacaggtga tgctagtccc cctgttaacc aagtgtatag acctgtctac 1080
catcactggt ttcatcagag agaaatagag ggaaggaaat tatgggaacc gttttctatg 1140
ttggattcat tagctttaga acaagctttc acttcaaatg atttagatcc agataaagtt 1200
attgctactg acggcggtcg ttatgatgtg aatattttaa gaagacaaag gaatcctgtg 1260
tattggaaaa cgaatcccaa tgaagtacga agatgttctt ggttccacaa agccagttca 1320
gacggaaggt tggtcccata tgaagaaaat atcgctacaa agttagaaga agaatacaaa 1380
tctgcgtttg aatctaataa gtggcacaaa acaatcgaat tacctcaagg agaaagtatt 1440
gtctttcatg gacctgatgt tttagtactt ttctctccca cgcaggttcc tgacgcatgg 1500
ggcaacacaa caaaccagtc ccgaccgaga gttgtaaaaa ggggtatgga tgaatttgat 1560
atagatgaag gagaacctgc tcaagtcgat cacatcttgt tcatggtaca tgggattggt 1620
tcagtctgtg atttaaaatt tagaacagta gaggaggtag ttgatgaatt ccgcagtatt 1680
gcctttcagt tagttcagtc tcactacaga tcgtcctgtg accaaggaac agtcaaccgt 1740
gtggaaattt tacctataag ttggcacgat aggctacatt ctgaagaaac tggaatcgat 1800
gatcaattaa aaagtattac tctggagagt atacctagac tgagaggctt tacaaatgac 1860
actttgctcg atattttgtt ttatactagt cccatttact agtcaaaaaa ttactacaac 1920
tgtgggaaca gagcttaata gaatatatga cctttttaaa caaagaaatc ctgattttaa 1980
agggggtgtt tcacttggtg gtcacagttt aggcagcctt atactcttcg acctcttgtg 2040
ccaccagcac tctaaacctg aatctgacgg agaagaaagt gatgaaattc tttctagtag 2100
tccaggctcg gtcaaaccag ctccacctca gcgaaagatg agtaaaagaa taagctatat 2160
gatgggtgct atcggtacgg gacagccgga aatacactac actcgactta atttcgaacc 2220
aaagaatttc tttgctttgg gatctccaat aggtatgttc gtgatcgtta gaggtctgga 2280
cacattaggt gaacactttt cgttacccac ttgtccagcg ttcttcaaca tttttcatcc 2340
atacgatcca atagcataca ggatagaatc tttaatcaaa ccagaatttg caaagttaaa 2400
accagtattg attccccacc ataaaggaag gaaacgaatg catttagagt taaaagaaac 2460
gatgacacgt gttggagctg atttaaagca gaaagtaatc gattctatga gaagtacttg 2520
gaattccgtt taccaattgg caatgtttca caaacagtcg ccccctagtt tggaagaaga 2580
ggtgtctaag gcatttaaag atcaaatggg gcaactggaa aacgagtcca tcccagaatc 2640
tacgggaact cctgtaggga atttgaatag tggaagaaga gttgattatg tcctgcagga 2700
agcacctttt gagttcttca atgagtacgt gtttgcctta acgagtcatg tttgttattg 2760
gcaatcggag gacaccatat tgatgatgtt aaaggaaatt tatacttcta tgggagtaac 2820
ttctgacagt caaattcctc agcaaaccat gacaatagag agaccaccat catctcctac 2880
agcaacaagg tccccaaatg ttcaaattcg tgcattccaa caacctattg gagttgatcc 2940
aacaaagccc atgcaattga actcgtctct tgaaccacca ccagtaagtg gctttgttcg 3000
aaaaacatag tatatgctct acgagtggtt gtacaagcca cttgccgaac gttatgctcc 3060
atatttgttg cacagtaaca ccaactctaa aattacaaaa aaaaaaaaaa aaaatgcgag 3120
<210> 176
<211> 275
<212> DNA
<213> 玉米根虫
<400> 176
tgctcaaatt tctcacccac aaattaagaa ctcattctat aaatgaggat aactatgcag 60
ggaaggccga tgaagaccat gattctggta cggaatcaga tgaagagatt gatgctccag 120
tatcaggatc agcatcctgt tcagaacgcg tttgtaacgt atcaccggca gacacaggat 180
gttcgatgga aagtccagct cctgatcaac acgatgctca cagctccgaa gaagagttgg 240
aagttatcaa tagcaatagt aaaatgaggc gatca 275
<210> 177
<211> 274
<212> DNA
<213> 玉米根虫
<400> 177
tgattccttt atcttttgta aataaaaatt ctggccaaat tatattagca ggtgatccta 60
tgcagctagg gcctgttata ttatctaaag ttgctaccga atgtggacta gcagagtctt 120
atttggaacg aattatgagc agatttccat attgtcgtga tccagaagga tttccagaaa 180
cgtctggatt tgatccaaga tttgtaacga aattattata taattacaga gcccttccgg 240
acattcttac tttgtacagt acactctttt atca 274
<210> 178
<211> 275
<212> DNA
<213> 玉米根虫
<400> 178
tggcctccat gccgagcaag actcccgtca gcgtcctcca ggagttgctg agccgtcgcg 60
gcatcactcc caaatacgaa ctggtccaaa tcgagggcgc catccacgag ccaatcttcc 120
gctaccgcgt gttccttaac aacgatctgg tggccaccgg aaccggaaga tcgaagaaag 180
acgccaaaca ttcggcagcc aagaacttgc tggatctctt ggtcggaaaa gtgactcccg 240
aacaagccaa tcagaccaac ggaacgcccg gagcg 275
<210> 179
<211> 273
<212> DNA
<213> 玉米根虫
<400> 179
tgaactccag tgaatcctta aaattagcaa aatatgaact atttccactg agcgatgtac 60
aactcggttg gaataactgt gattggtctg ttggtgctgg aatggttaat atgggaaata 120
catgttattt aaactctact ctgcaagctc tctttcatgt accagctttt gttaattggt 180
tgatgtcaga caaagatcat actgccatct gtcaagattc aggaggtctc tgtataatct 240
gcgcgatgcg caaaactctc caagactcac aac 273
<210> 180
<211> 273
<212> DNA
<213> 玉米根虫
<400> 180
tgggtccaca tggagatgta ccacaacaac ctaaatcggc aaacgggcaa caaaggggtg 60
gaccacaaca acagcgtagg cctcaggata ggcagcagac agcttctgtt aaagaattga 120
catctcgtat gagtgaactg caagctggtc cacttgtacc tatgaggtta agaaaccctg 180
aacctggaaa agcaggtcgc aaaatacccg tagaaacgaa tcatctcagc ctcgcgctag 240
gtaaattaaa cacggcttat cactatgatg ttg 273
<210> 181
<211> 272
<212> DNA
<213> 玉米根虫
<400> 181
tgagcgagaa ggaagttaat gctaagatcg aggagttatt gagtgacatg aacctcaacg 60
acgacaaaaa gaaacccctt agagaaatgc ccatggaaca gaaacgtgca atgttgaaga 120
tgcagaacaa aaagaaagga caacacgaca cgggcagtcg attcaacgat cccgaagatt 180
attacagata tctggatcaa cacataaatc cggagtactt gcagttgaac aaatgcctga 240
actgtgtgga aagcttgagg gtagccttga ct 272
<210> 182
<211> 275
<212> DNA
<213> 玉米根虫
<400> 182
tgctgttttc taatgagatc tgggcggcta caaaacggca ggcattgaaa aggacgaatt 60
cctgccagga tagaattgaa aggatccctc ctagctcgga agaacttttc gaaaatatcc 120
cttggatgaa tatcatggct gatctgactg ttaaatctgc tatagctgaa tatgagttta 180
aacgtatgct gaataaggag ctgtctcatt tctccgaatc tagcaaatct ggcaatcaaa 240
tatcggaata tatctgctcc actttcctag acaaa 275
<210> 183
<211> 275
<212> DNA
<213> 玉米根虫
<400> 183
tgacagtggg gcgattggaa aaaacgttta cagcattgcc aacaatttat agtaagaatt 60
gtgtttgttt tgatggcatc tacaccaacg accaaccaat gcaccgccag actgagagtt 120
ttgtttacgc tgacgacctc ggtatcgccg taaaagagaa aacacataca caagtaaagt 180
cgacaatgga agaggcttta aacgtgatgt caacttacta caaacaaaac tcattaaagc 240
taaaccctac taaaacccaa gtatgtgcat tccat 275
<210> 184
<211> 275
<212> DNA
<213> 玉米根虫
<400> 184
tgtttagcac acaattagga aattttccat ttgctaatac taggaacagc caagagacaa 60
cttccaacag tttgaataat catataatgg atgccaacaa tggagctgga gatagtaatg 120
atggttccat agataaaacc aaactaatta ttaattacat accacaatat gccactgaag 180
gtgaactaac tcaaatattt tcaaccgttg gaaaactgga agatgtaaaa attatgagag 240
actttaagac tggttacagt tttggatttg gtttt 275
<210> 185
<211> 275
<212> DNA
<213> 玉米根虫
<400> 185
tgagagcaag acagtacatg accgaaattg ttggaggtga aggtaacgtt ttgtgtcatc 60
acatgaagta tgaagatttg gaggcgaaat tcgaaaaaat tcaagataaa gaatccttac 120
gaaaattctt gaagcaagtt acatacgata aacaaggtgt tctccactta ttcccctggt 180
cgggtattat tgacgagaac caagagttga gtgcttcctt cagagttcca gacttaaaat 240
caggaaggat gatcaggcta ttaacacagc tatca 275
<210> 186
<211> 275
<212> DNA
<213> 玉米根虫
<400> 186
tggatgccat accagctagc gaactatcac aaaatgctaa tttcagtagt tccctcgtga 60
attctccaga gtctctgcca aaaaataata acacaggtga tgctagtccc cctgttaacc 120
aagtgtatag acctgtctac catcactggt ttcatcagag agaaatagag ggaaggaaat 180
tatgggaacc gttttctatg ttggattcat tagctttaga acaagctttc acttcaaatg 240
atttagatcc agataaagtt attgctactg acggc 275
<210> 187
<211> 2593
<212> DNA
<213> 黄瓜十一星叶甲
<400> 187
acgattcgtt tggtagtaca tagagaaatc gtttttagtt ttataacctt ttctgtgttg 60
gaatattgaa caaattctaa tgattcgtaa agtaacactt ctgctaaaaa ccaaaactga 120
tagttttttt caaaaaaagt acaatttttc tgggcttgct actgaaataa tgtgccatac 180
tctaattgtg gaaaaaattg taggtatatg ttgaaagtta acaaataact aatgcttaca 240
ataatgtcga catcaagtca aaataatggt aaaaagagtt cagccagctc tattgttaac 300
accccttcca gcactcccat tactaatctt gcaaatggta gtagtaccca aggaacagca 360
catagttcac ctccacctaa tagcaatgct ccaaagtatg gtaccctagt gcctaacaga 420
atattcgtgg gaggaatatc agctaacaca acggaaggag aattaatgca actttttagc 480
aactatggta ctgttaaagc tgctaaaatt attcaggaca gggctggtgt atcgaaagga 540
tatggtttca ttacctttga gagtgaggat gatgctaaaa gacctcttag agaagctgaa 600
aacatagtgc taagagaaag aaaacttaat atagcacctg ctattaaaaa acagcctttt 660
agtcgagcat ttgatgcatc cagtccacct gctgttgctg caaataatgc cagtcaattc 720
ttttttcctc cgggtgctgc cgtgccatat tttcagagtg gagttacata ttatactcag 780
ccagcccctg ctgcacctgg agatcccact cctcaacaac ctgtttatca acctcctcca 840
atgtatccca cacaaactgg tcctccacag ccagctacat acccttctat gatgtttccc 900
gctcaaacta tatatatgcc acaacagtat cccatgccta ttccgtacga gtacaacttc 960
taccagggta acggcccatc gctgccaact cagtatctgg ccggaggcca aagtggttcg 1020
ggaagctctc attgcacctc attaccatca tcaaacagtc cacccagacc caatggttat 1080
ggtcaacagg tacctaccta taattcagga gatcccgtct actacaactt accaatctac 1140
ggagctactt tggaaggtcc gccattatac gctgatgcct tcgatctaag cgctagtgga 1200
acttatgcgg aggaaaccta cagcagcatg attacaactc ttactcaaga aaatgtagaa 1260
gcttctcttg gatcatcgat aaattcaact catgcagctt ccgacaattt cacacaacat 1320
ctggatcaat ctctcgttga gtctgattca agtattgtga atgttggagg taaaccacca 1380
ctacataaat cctcagagac aattgtttct gctctttccc aacctcagga ggatagaaac 1440
tcacatacgc ctattgtttc actactttcc atagatcatc aacaagaaaa agactattca 1500
tcaatgcaga gcggtcgtag acgtaaaatt ctggttcaaa gcaatcaaaa taacgtgccc 1560
atgtatccta caaatggata cgtcaaccag ttcgtcggat ataatcctca acaaccaccc 1620
ccacattcat tcggtcaacc tctctttaac aacggttacg gtttttcgga atatcgtcga 1680
tttaacggat acccagatat caggtcaatt aataatagta ataaaccgag gagacgaata 1740
tacgaaaata ggcggtcgaa cgatcattct agccgttcta gtcttcgcac agactcaaac 1800
tcatcggcaa gttgtgtcga cgaaaataaa aatgaagaaa aaacttttcg aactactgtt 1860
aacactcctc cacctgcccc atactcacct atgactaatc aacaattcaa atttattaat 1920
tacagtaaca cgaaaaacat ttataatcat aagtacgcta ataacaataa ttattataat 1980
agaactgcta cacaccatga ccgatttaaa cataccgaac ctctagcgaa taaaaacaat 2040
tacagttcca tttatccctt aacaaataat ttcctaacta atagccatac ccaaagtaat 2100
tctgattccc ttccctccca aaattcccag gtacatgtga cgactacggg tcagagcttc 2160
gtcccggtga ctatccaagc tcagacgaaa cgcaacaagc gttctttgag acggagcggt 2220
gccagtgctg gaggcatcaa cgaaatcggc gccggtgacg cccctttacc aggcgaagaa 2280
tgcgaagacg tctacaagaa gctggagacg cttaagttgt aacgaaaaag tggtatattt 2340
tcttgtatat ttttttctat ttatttaaga ctgtacaata atgtaaaata ctagaaattt 2400
aatgtaaact aaagaggaaa aatatataat acatatttat taaagatgat ggagatgtaa 2460
caagttttta tttattaaaa acttattttt attcgttgtt aacaaatggg agctgatttc 2520
ttcaattata acgtgttggt atcttgtaat tgaaaaacgt caattatagt acatattatc 2580
gtacaattat tag 2593
<210> 188
<211> 2585
<212> DNA
<213> 马铃薯甲虫
<400> 188
gccgttgaac agtagtgaaa atgtttagtt tttggatgat ttgtgtattg agttaacaag 60
aatattctaa taatacaaga atttatgaga acgtctttcg atacatctaa cggggcttta 120
cttaatcact agtgaaacca gacagttcaa tttttcccgg aaaacaatag aaatgtgttc 180
taacctaatt gttgtatgga aaaaattgta ggtatctcct gaaagtgaac gaataaggaa 240
aagctgaaaa atgtcgacgt caagtcaaaa taacggaaga aagagttcaa acagctctat 300
tgtcaacaca ccctccagta cgcccctcac aaatgctgct aatggaaatt cgcaagttag 360
cacacaaagt tctccaccac ctaataacaa tgctcctaaa tatgggactc tagttccaaa 420
ccgaatattt gttggtggaa ttacggccaa taccaccgaa ggtgatttaa tgcagctctt 480
cagtagctat ggaactgtaa aggcagcaaa aataatacag gatagagctg gcgtttcaaa 540
gggatatggt tttataacgt ttgaaagtga agatgacgcc aagagaccat taaaggaagc 600
tgataacatt gttttcaaag aaaggaagtt gaacatagca ccagctatca aaaaacagcc 660
attcagtcgg gcatttgatg catccagtcc accaaatgta gcagctggaa accctgcaca 720
tttttttttc ccacccggag ctgcgatgcc atattttcaa gggggtgttg cctattatcc 780
acaacctcca cctgctgctc ctggagaccc aaatgcccag caacaagttt accaacctcc 840
cactgtgtac cccactcagg caggccctcc tcaaacggcc acctatcctt ctatgatgtt 900
ccccgcacag ccaatttaca tgccgcaaca gtatcctatg cccataccgt atgactacaa 960
cttctaccag acaaatggag ctccgccctc cgcacagtat attgttggag gccagggtgg 1020
ccccggagga ccacaatgtg gcccaatcgc gccttccaat agtcccccaa ggccaccttg 1080
ttatagccaa cagatgccag cctataatcc agcagatccc atgttctaca acatacccgt 1140
ctatggtccc acaatggagg gaccacctgt ttacaccgag gccttcgact tgggaaatgg 1200
tggaccatac gcagaggaca cctacaacat gggtgataac aacattacac aacaaggcat 1260
agagacttca atcccgcctc ctgtctcagt acctgctgta caaacactca cttttcctac 1320
tcataacatc aatgttcctc ctcctcctat tcctacaagc attgagttga atgtccccaa 1380
tgcctcttct cggcaccaga tggatcctgt tgttgcagaa gatgtgtcta cacctaggaa 1440
ttcagacaca cctgtagttt ctatcctttc tatcaacgaa cagcaagaaa aagactattc 1500
atcaatgcag ggtggtcgaa gggttaaaat tcccccgcca aacctgcata acaactttca 1560
aattcagcac gctcaacatc caaatggtta cattaaccat ttttctggtt acaacggtcc 1620
accaccgact cagtttggaa tgtacaatgg ttattctcag aatggtccca ttttcccgga 1680
ctatcgtcga gtgaatgggt ttgacaaccg tatcaacaaa aatagaaggc gttcatatga 1740
caatcgaaaa tccaacgatc actcaagccg ttccagtata cgcactgatt ccaactcctc 1800
ggcgagttgc gttgatgaaa acaaaaatga agacaaatct cacacgcgca cttctgtcaa 1860
cacgccacct cctgcccctt attcaccgat gacccatcaa tttaaaacta ttgcaaacag 1920
caacacaagg aaccaccaaa attccttcag atattataat aataataatt attacaaacc 1980
agcatattta gaccgatcaa aaacaaatga cttgaccact cataataaac ccagcaacaa 2040
ctcctctagt aataacagca ccttttcttg cccaaactcc ccacaatcct ccatttccca 2100
ggtgcatatg tcggcaactc agaccttcgt accgcaagct aggcgcaata agagatctgt 2160
tagacgtagc ggcaccggcg gattgaacga aattggcgct ggcgacgccc cccttcccac 2220
tgaagtcggc ggtgaggtgt gcaagaaatt ggagacgctc aagttgtagt aggtgggaga 2280
aagtcattat tatgtatatt tttttctatt tatttaagac tgtacaataa ctgtaacata 2340
ttataaattt aatgtagaaa catattataa atttaatgta gacctatgag agggatatta 2400
atataatata tatttattga aaaatgtgta gatgtgacga tttgattttg agaaaggatt 2460
ttttttggtg gtacagatga cttggaaacg tcttgtcaga tatcttaatg tgtcatttct 2520
atccatgata aataattgat gacttttcat attgtgaact ttgttcaaga cgcgtttcac 2580
tgtgc 2585
<210> 189
<211> 2154
<212> DNA
<213> 萝卜菜跳甲
<400> 189
gtacgatccg ttttcgtagg aactagtgaa attggttttt agtttttcgt ccttaatagt 60
gttgaatttt tgaatgcatt ctaatgattc gtaaactaac gctgcattta aacgacgtat 120
attttgaaag cgaacgaata acgaatgccc tcaaaaatgt cgacctcaag tcaaaataat 180
ggtaaaaaga gttcgaacag ctctattgtc aacacaccat ccagcactcc tgtcacgaat 240
atcgccaacg gtaccaccca agtgagcacg cagagctcgc cgccgcccaa caacaatgct 300
cccaaatatg gtactttggt gccgaacagg atcttcgtgg gcggcatatc ggccaacacg 360
accgaaggcg agctgatgca attgttcagc gcctacggca cggtgaaggc ggccaagata 420
atccaggaca gggcgggcgt atcgaagggc tacggtttca tcacgttcga gagcgaggac 480
gacgcgaagc gacccctacg ggaggccgag aacatcgtgt tgcgcgagcg gaagctcaac 540
atcgccccgg ccatcaagaa acagccgttc agcagagcct ttgacgcgtc cagtccgccg 600
acggtggcgg cgggcaatcc cacgcagttc ttcttcccgc cgggagcggc ggtgccgtac 660
ttccagggag gcgtagctta ctatccccag ccggctccgg ccgcccccgg ggacccgtcg 720
acgcaacagc cggtgtatca acctcctcca atgtacccga ctcaaacagg cccaccgcaa 780
acggccgcct actcgcccat gatgttccca caaactattt atatgccgca acagtatccg 840
atgcccatgc cgtacgatta caacttctac cagggcaacg gagcctcgcc ctctgcccaa 900
tacatggcgg gagcggcgca aagcggatcg gcggcgggac cgccgcaatg ctcgacgatg 960
ccgccttcga atagtccgcc cagaccgcct tgttacggcc aacagatgcc tgcctacaat 1020
ccgggggatc ctatgtttgc ttataatttg cctatatacg gttcaacgtt ggaaagcttg 1080
ccaatctaca ctgaagcttt tgatctcggc gcgagcggtt catacgctga ggaaacaatc 1140
tacaacaatt tggatcgaac ggtaaccccg tcgcacgacg acgacccgac caacatacag 1200
acggtttcct actgcaacac gacaaacaac acgaacggcg gcaccgccgc ccccgaccca 1260
gcacaacaac aacaccccgc gttcgtcaaa gacgagagaa acaacgcgcg cacgcccgtc 1320
gtttctctcc tctcgatcga ccaccaacag gagaaggact actcgtcgat gcagagcggc 1380
cgccgccgca aaccgccacc cccgccgcag ccacagccgc cgccgccgca gctacacaac 1440
caccacaaca acccgaccta tcaccaccac catcacggcg cgttcgtctc gcattcgcat 1500
ccgttcgtcg gcttcaatcc gcaacagccg cctccgttgc cgccgccgcc cgccaacggc 1560
tacgcgccgc caccgcaggc tatttacaac gactatcaca ggcgcgttaa cggttgtttt 1620
gttagtaata gcgtgaagaa tgtaaataat aacggtaaaa atcgcaacga ggacggtaag 1680
agtaccgtac gaacggcggt gaatacgccg cctcccgccc cttactcgcc gatggcgaat 1740
caccaattca aagtttacac gaacagtagc gttaagaatt ttatgaacaa taataacagc 1800
aacaagaaca ataattacgc tagaagcaac aaaaagtacg ctgtaatggg cgtgccgcct 1860
ccaccgccgc caccgcccgc ctctaacgca tccactacga ccccaacaac atccaccaac 1920
gacaaaatct actgtaacaa caacaataat aacataacta gtagtagtag tagtaatagc 1980
gccgccttgc ctacgcctgc ttcctataat tcgaatacta ataataataa tagtagccgc 2040
ctccttcact ataataataa ccattcccct tcgattaacc acgtggattt gggggtgacg 2100
caggtgcacc tgtcgacgac gacgtcgcag agcttcgggc cgcagacggc gcgg 2154
<210> 190
<211> 2036
<212> DNA
<213> 黄曲条跳甲
<400> 190
ttgaaattgt ttttgaactt ttgctgtttt gtattcgttc tattttcatt ttaaacggtt 60
taaaatgctt ctctgagtat tttaaaataa gcaactaaaa acaaattcga atttttaaca 120
attttggaat cgactaaacg taaggtatat tttgaaagcg aacgaataac gaatgccctc 180
aaaaatgtcg acctccagtc aaaataatgg taaaaagagt tcgaacagct ccattgtcaa 240
cacaccatcc agcactcctg tcacgattgt cgccaatggt actacccaag tgaatacgca 300
gagctctccg ccgcccaaca acaacgctcc caaatatggt actttggtgc cgaacaggat 360
ctttgtgggc ggcatttcgg ccaatacgac cgaaggtgag ctgatgcaat tgttcagcgc 420
ctacggtacg gtgaaggcgg ccaagataat ccaagacagg gcgggcgtat cgaagggcta 480
cggtttcata acgttcgaga gcgaggacga cgcgaagcgg ccgctgcgcg aggccgagaa 540
catcgtgttg cgcgaacgca agctcaacat cgcaccggcc atcaaaaaac agccgtttag 600
caggaccttc gacgcgtcca gcccgccgac ggtggcggcc ggtaatccga cgcagttcta 660
cttcccgccg ggggcggcgg tgccgtactt ccagggaggc gtcgcttact atccgcaacc 720
ggcccaggcc gcgcccgggg acccgtccac gcaacaaccg gtctatcaac cgcctccgat 780
gtacccgacg cagacggggc cgcctcaaac ggccgcctac acgcccatga tgttcccgca 840
gatttatatg ccgcaacagt atccgatgcc catgccgtac gattacaact tctatcaggg 900
caacggagcc tcgccctctg cccaatacat ggcgggagcg gcgcaaagcg gatcggcggc 960
gggaccgccg caatgctcga cgatgccccc atcgaatagt ccgccgagac cgccttgtta 1020
cggccaacag atgcctgcct acaatccggg ggatcctatg ttctataatt tgcctatata 1080
cggatctacg ttggaaagtt tgcctatcta cactgaagct ttcgatctcg gcgctggtgg 1140
ttcgtacgcg gaggaaacaa tctacaacag tttggatcga acggtaacgc cgtcgcacga 1200
cgacgaatcg accaatttgc agacggtttc ctactgcaat acgacaaaca acaacaacaa 1260
caacacaaac ggcggcgccg ccaccgccga acaacacgcg tcgtacgtca aggaggagag 1320
gaacaacgcg cgcacgcccg tcgtttctct tctctcgatc gaccaccaac aggagaagga 1380
ctattcgtcg atgcagagcg gccgccggcg caaaccgccg ccgccgcaac cgccgccgcc 1440
tccgccgccg caattacaca acaacacgac cgcctatcat catcaccatc acggcttcct 1500
gccgcattcg catccgttcg tcggtttcaa tccgcaacaa ccgccgccgt tgccgccgtc 1560
gaacggctac gcgccgccgc aaccgccgcc gccgcctatc tttaacgatt accatcggcg 1620
cgtcaacgtc aacggttgct tcgtcagcaa caacaacaat cacattagcg gcggcaagat 1680
tgttaacaac gcgaaaaatc atcgcaacga cgatggtaaa agtaccgtac gaacggcggt 1740
gaatacgccg ccgcccgccc cctactcacc catgacgaat caccaattta aagtttacac 1800
gaacagtaac gttaaaaact ttatgaacaa taacaacaat agcaaacaga acaataacta 1860
cgctagaagc aacaaaaaat acggatcggt gggtgttcct acgccgccac ctcccgtttc 1920
taacaccgcc tcgtccacca ccaccaccac caccgccgcc acctccacat ccacatccac 1980
atttgtaacc aacgacaaaa cctattgcaa taacaacaac aatagtacta gtagta 2036
<210> 191
<211> 4893
<212> DNA
<213> 十二斑褐菌瓢虫
<400> 191
gtcgtcgcca ttttcgatgg ttccgttttg tagtgcaaac ttcgttagtt tttgatagtt 60
ttgtgtgtta ttttatcaaa attcactaat gaatcttatt aaactatcgc taaaatcctt 120
tgaagtgttc ctttgcaggt agtactcgag agtggaagag aaatttaaag cttctaaaga 180
tgtcgatacc taatcagcct aacggtagaa agagttccaa tagttctgtc gcaaacactc 240
ctgccagcac ccccgtagcg aattcgaatg ggtctgtcca aacggtcgtg cacaactcgc 300
ctccacccgc caacaacgct ccgaaatatg gtaccctggt gccgaacaga atattcgtag 360
gcggcatatc ggccaacacg accgagggcg agctgctgca gctcttcagc tcctacggca 420
cggtaaaggc ggccaagata atacaggaca gggccggcgt gtcgaaaggc tacggtttca 480
tcaccttcga gagcgaagac gacgcccagc gcccgctcag ggaggccgat aacatcgtgt 540
tgagggagcg caaattgaac atagcgccgg ccgttaagaa gcaaatacct tccccgcagc 600
ccttcagttt caacagagcc ttcgacggtt cgggatcgac gacagtggcg gtcggtagtc 660
cccagtactt cctgcccccg acgatgcctt atttccaggg cggcgtgagt tactacccac 720
cgcccccgaa cggtccggtg cccggcgatc cgaacgccca gcagccggtc taccagcctc 780
cagccgttta ttcggcacag gccggcccgc cgcagcccgc cagttatccc tcgatgatgt 840
tccccgcgca gccgatctac gtgccgcagc agtatccgat ggccttgccg tatgaattca 900
gctattatcc gaacgccgga gcccccccgg cacagtacgt ggtcggtacc caaggcggtc 960
ctccgccgcc gcagccacca ccaccaccat cagcacaagg tcccccgctg cctccgtccg 1020
gtagtccccc tcgtccgcca ccctgctaca accaacagtt gcccttcggc gccgagccca 1080
ttttctacaa cgtgcccatg tacggcggac ccatggatca tccgcccgtc tacgccgaac 1140
ccctcgaagg cgcaccatct aattacgccg aggaacaatt cacaatcgtg cctcgagccg 1200
gcgcatacga ggtagacgcc atgtacaacg gccgtcccgg ctccggcttt aacgaatcga 1260
cacccgatcc atctttatcg cacaacgtgc cgccgtgtcg gtacagcaac aatacgacgc 1320
cttacgctga tcaacagcaa cagcagcagc tgcaacaaca acaacagagg gattcggagg 1380
cggacaacct aagacagtcg aacacaccgg tcgtttcgtt gttgtccatc gagagcacca 1440
tggagaagga cgtgaatgcc atgcagtgcg gaaggaggag gagaacgtat gcctcctgca 1500
gtcccggtcc gtttttcggg ccgctaatgg gcggcatgcc gcccatcatg ttcccgcctc 1560
cgaattatcc cccgcccccc atcaactatc atccgcacgt tcccttcccg caatttccca 1620
accatcggcc gttcaacggt tacgatagcg ctcggccgaa gctctacaac gccaacggct 1680
actcgaagaa gcagcagaag aaattcaaca ataattatag accggtggac aactcgaaca 1740
ggtcgagcct gaggacggag tccagttcat cggccgccag ctacgcggaa gacgcacgcc 1800
acgcccacca gcatcgcacc aatgtgacat cgcccccgcc cgccccctat tcgccgctaa 1860
cgaaccacgg tttcacgtcg ccgatcaacg gcatctcgaa tccgcccaac gttccgtaca 1920
acaacgttcc tcatccgcga cctagaaact attacaacaa caataataat aataacaacc 1980
acgcgcatca cgataagaaa agaaataata acaataaatc tcgaacgtcg aatggtaacg 2040
gttaccacaa taataagaac aataatcaaa ggggcgagac cagcccagtg aatcagactc 2100
atgacacgaa tacatcgcag gtccataacg gcgggagcgc aaccggcttc gcggcccccc 2160
tgcccagtcg caggaacagg aagaacgtgc gcagaagcaa cgtcgcggcc acagtggcag 2220
cagcagcagc cggcgccgcc ttgagcgaga tcggcgccgg agacgcgccc cagcagtcgc 2280
aagaaccggt gtcggaagcc tgcaagaaat tggacagcct caagttgtag aaaggtaaaa 2340
aggggatgcg tgtgttaatt tttttttctt ctttttaatc gatgccatcg ccattgacat 2400
gaaaaaacca caacaaacga ttatcttcga ataagactgt aaattttata tataacaaga 2460
aattacttga tattacgtaa gaaagggaaa tattaaatat ttattgtaaa ttcaaagtat 2520
tatttttttt gttttgtata ttttaaatta ttatttagaa aaaaaatgaa gcaagttgtt 2580
caccgtagga catcactttg tatataacga taataatgat gcaaaaacga aaaaaatctc 2640
tagctatggt atatgcaata tttatttaga gtataaacga caatattaat tgtatatgta 2700
atacaaaaat taaacgagat gccatattgg actagatatt aaaatgaaat cggtaacgtg 2760
ctgaaaaaaa aagtagactc gcgtttctcg gtttagatcg aaatcgtgcc gcgccgcatc 2820
agacaaaaat tgttatttaa tatctaagga tcgtgcgtcg ctctgttttc gttttctttt 2880
tgtttctttt tttttttact tcaaacgcgt cgggtcctct tgcgctcatt tcgtatacaa 2940
ttatgttttg acgtttctcg acgttagcaa tttctcaatg aaaaaaaaaa gatctctgaa 3000
gcgaccagtt gtcaattcta ctcggtgatt aaaaaaaaaa ttaaatttaa aacgaaaaaa 3060
attcggttcg tctagacgaa aggagaagtt gtgtgcgaaa caccgttctg ccttattttc 3120
ctcttctctc ggtggttaga ttaggttttg atagaaaata ttcgttttgt ttattttttt 3180
tttgtaattg aattttttca cggtagtttg acttgtatat gcataacaaa aaggtgccgt 3240
gatctgctcc atcgcgtttt taacctcaaa cgtcatatct tctgtcaaaa cctccgtcta 3300
cgccgtcttg cgaattatcg aaatttttct tgtcaagtct tattcgaatc tcccccctcg 3360
gacggaacaa tggagcatcg tccgcttttt gttgtgcgta cttaacgaga ttttgctcaa 3420
aacggctgcc atcgtctttc gtacgttgtt ttattgaggg tgcgcgatcg agggactcgc 3480
gacatccacc cgatacgagg agggtagttt tttttctttg ttccctcgat tttccgagcc 3540
gtctgcaagt ttccatcatc gaacgtcgat gagttctctc tttctctctc tctcccccct 3600
ccagttaccg gtacgttcga agttttgtgt cattttgaat tttaggctgt gacataaagt 3660
tatttattgt tgaatttggt agatttttta aggaagagaa tctgtgtttt ttgtagctat 3720
ttattcgata agtttcgacg aaatcggttg atctcttgga acccgactcg gctttgcaaa 3780
ttttttccgt tttgacaatc gtcgttgcgc ggcctcacct tgacgactcg acgtcgcggg 3840
ttccaagact gcgcgcaggc tcgaaacgtt tttaggatca tccggggctc gtttggatta 3900
tttttcgtag attttttttt cgcgctcgaa cgccgagata tttcgtagat ggacgaattc 3960
gcccccccca aacccccttc gaaccccccc aaaaattcga tgcgccagtc ggatcggatg 4020
tttcaaatgg ccctaagcga tcgagaaata caaaaaattg atgtgaaatt ttggtttccc 4080
caaagaaata tgtaaacttt aggctcatca attgatattt atccaaatta gatgtgtaag 4140
ttagtattag taacttagtt gtagttttag gataaatgaa ctatctctta ttcaaataga 4200
tgataagacg gcaattgtct ccgttcatca ttaaaacata caggattttg ctcaaagaac 4260
ttttttcgtt ttcttcttaa aaatgtgata aaaaaaaacg tgaggcaacg agaggttctt 4320
gtttttttct ctctatctct ctcgttcgta gggaaatgct tcctcatgtt gcaaagattt 4380
cactgagctg tgtaaatacg ttcattttgt tatattgttc catttttttc tctccaaaat 4440
caaagggcag ggtcggttgt tgttgctgtt gaaattcctt aattttttct tcaactccct 4500
cgaaatcttc aatctgacga aaatttattg aaaaatatga tatttccacg tttccattga 4560
atttatcgat gtggtattaa tttacttctt tcttttttcg agattattgt gtgaaaatta 4620
aattttcttt ctatgatgtg tggagttgaa gaaaaacggt cttttgtcat ctaaccgatt 4680
tgtgtgaatt tcgtgtccgg ccctacgtcc atttttttcc aagaatatct caagattttt 4740
cgcagcagta cggtcttcaa accttgaacc cttcattttc ctctctcccc aaaccctcgc 4800
ctcgattctc tttctctcac acacacacac acattgaacc gatttgaaat agtacctgca 4860
aaatattttg taaatattct gtttgtaaat tac 4893
<210> 192
<211> 2051
<212> DNA
<213> 狡小花蝽
<400> 192
tttttttatg ttactttgtc ctttaatatc atactcttaa aaatcaaata atcctttaaa 60
tgtcttaatt cgtctattat tttttctttc ccaccatttt ctttatcata aatttttttt 120
tggaatccat ccgaaaggtg aacccaacgc ccgccattgt tcgcactacc cccatttttc 180
gggccgactt ataaaattcc gtcagaaaat attaattttg tcaaaaaacg agaatctatt 240
tagaacatga gaaactcttt tcctttcgga actcgcgtta tactttaaaa aatttaaata 300
tcaaataagg atggcggttc aaaccaacag agggatgacg agcgaaaatg aaatgaaaga 360
tcaagacgtg gcgccggtct ttaacatcaa cgcacccaaa tatggaacag tcgtgccaaa 420
cagagttttt gtcggtggca taacatctgc gacgactgaa gacgatctca atcagctttt 480
ctccaactac gggaaagtgg tcaacgcgaa aataatcgtc gaccgtgccg gcgtctctcg 540
aggttacggc ttcgtcacgt ttgaatccga cgaagaagca aggcgccttc agaaagatgc 600
tgataacatt gtacttaaag aacgaaaact aaatatcgct cctgccatta agaaagtcca 660
gcctgtctac acaagaggtt atgacccttc aagcggacct ggaagccctt ctcaatattc 720
aagctacatt caaagacaaa acggagtcgt cagctcatac tacatgaaca gtctaacgta 780
ctttcctcaa caggcagccc aacaatctgc accttcgatg accccgttac cttccaacca 840
tgtagaacct tccgtttatc ccggaaatta cccagcgatg cctcagtgta attcgcctcc 900
aagttatcaa tatggatatc ctcagcaagc gccgatcttc attcctcatt atccgtttgc 960
tatccctcag tatgatgggt gttatcaagg agaggtacaa aactgttatg ctcaacctgg 1020
aatggaatct aataatcagg tcgcgtatcc gcaatatttt gtatcgcagc aacacccaga 1080
aataacgtat tatcaacagc cggtacctca ggagttggtt tatacacaaa gaacttatga 1140
ttcgtcgact gtatatttta ctgagcctaa gtttgacgat tcgtgtgaac catcaccgac 1200
atcgccaaat caacaatcac ctgggccacc aatgttacca cctgcaccct cgttgccgcc 1260
gatttccgaa atcgataagc caaaagcgag aaacgacgaa acgccagtcg tttcgctttc 1320
gaaagtatac cacgaccaaa acgacgacta ccagcccaat ccgagatata aaccccatca 1380
aggtttcaaa cggaaacctc ccccgaggca cgaaaacaac aacaacaatc cggatcttac 1440
ggataaaaat aggatgaatc gatatgataa aagtaggaac gttggtcaaa aacctgtccg 1500
tcctatggct tcgcagaatc gtagacccat ccacaatcca cgtcccacca acctctttca 1560
atatcgtaat caatctatgc aggtaccgca gtttccttca tactacgcct caaggccata 1620
tgtgaaccaa tcgcagactt actacaacca gcgaaaacca tttcaacgcg gcaaattcag 1680
tgcaaaggcg aaaaaataca gagacgagac agaacctgaa ggtgcagaag atgaagacgt 1740
ctgcgaaatc gatcagttaa ctcctcccat aacacccatt tcgccgaaac aacatgacat 1800
atcagaatcc gatcgtaaag acgaattaat ccgatctttt gaaaacttga aaatgtaaaa 1860
agcttatttt gaacttcaaa attttctaaa aatataaaaa gtgtcgaatt cgggtcacat 1920
taaaaatttt tgatatattt gtaaattttt ttctttttca gaaaaaatgt gccaaaacta 1980
aggtttgtga taaataagcc ataatgtaac tttgaggcct taaaattgtt tatacctcac 2040
ttcttttttt t 2051
<210> 193
<211> 2837
<212> DNA
<213> 豆荚盲蝽
<400> 193
ccgctgttag ttgggtggta gttatatttt ttgggggtgt tacctatttc ttcccttttg 60
ttttactcta agttggtggt aatttggatc cgaatttgtt ttatttggtc gttgaagaga 120
gtagtaaact tgtagattag tttttcaacc ccagccccca gttttaactc cctagtgcag 180
ctcgtcgtgc tgccgccatt ctgcttggac tgggtttttc gctgatttgc ttatctcgta 240
atatggacgt aatccggtgc cacctgatcg gaaatagttt ctaagaagaa cgtgttttgt 300
atcgagctcc tgcgttgtta attcaatcaa ctagtatcat ggagaaccct tctgaaaagg 360
atggaaagga ttcgaatgag tctcaagtgt cttctccggt gagcactgtc atcaatgctc 420
ctaaattcgg gtacgtcatc cccaatcgcg tattcgttgg agggatttcg tcgcagacaa 480
cggaaaacga tctggcccag cttttttcca gctacggaaa cgtagttaac actaaaatca 540
tagctgacag ggccggtgtc tcaaagggat atggttttgt gactttcgaa actgaagaag 600
aagccaaaag actgcaagca gatgctgagt ctatagttct ccgagagaga agacttaaca 660
tcgcccctgc tattagaaaa cagaactacc aaccgagaac ttatgacggc gtccctggaa 720
gcccagtttc tatctcacct ggtcagcttt acagacagac tggtgtaccg ttcttccaaa 780
atggaatggc tttttatact gcagctcccg ctccccagcc tcctcagcca atgacacccg 840
tgcagagtcc agtggaagct cctcaagtgt atccacctgg tgcttacggt ggtgtccccc 900
aaaattcaac tgcacaaaac ttcacttttt cctaccctgc tcaaagtcac gtctattacc 960
cagcccatta cccttatcag cttgctttgg aaggataccc tgtcactgat gtcaacaatg 1020
tgctcatacc cgccggagga ggtgctgaaa atggaggggt tgtctttgct gcttcaccct 1080
acatattcac tcaacagcct ggaacccaac aaaacgcctt ttttgatcct caaaacattg 1140
cacaaattgc tccgactcaa gaacaaatct tctatcctgc atctagaggc tacgagacgc 1200
ctacttttct atacactgaa caaagcgggc gtggaaagaa ccccaaagga accgggtggt 1260
acaaggacag taacgagtca gtgactgaag aagggaatgt gctggccaac gccctcaccc 1320
ctccgatcac ccccagatct ccccacgacg cctcggagga cctctccgcg tcccttcaag 1380
gtctcaacat ttagtcatcc agatttcgtg tcatttttaa ggcatatctt ccctctaaag 1440
acttgaatat tctaatacta atttcgttct agcacagtat ctttttgata gcaaataaaa 1500
atgattaaat ctctaagtgg ttgttggtaa tacggaagct acgaaatgga aggaacacat 1560
tgattagctt tcccacttca ctaaaaaaaa tttaaaaaaa cctcgggtgc cttattcatc 1620
ttaaaatgac tgacccaagt gccatacaaa ctctttctca tctaaatttc ctaacttttg 1680
ctaaaatttc aattttgatc taggaacgct aggaagctca acaccaaaag taaacttgac 1740
tgccctaatt attaactaaa accaccaata taaagtacaa tgacttcctc tggatacgac 1800
ggttaagact cgtcttgcct tgtccattta ccatgtaaat ttattttcga ataacttgag 1860
tatttttcta gaacgcttta acagtaataa atcttttata aatatagatt tatttctact 1920
ctaaggatag aattaccttt ttgtacctgt agacttgtta cagttataag taataataaa 1980
ttttataaaa gacaaagtca ttgttgaata gttcgtaaaa ctctttagta agttttctag 2040
ttgattgcgt tgaagcactt taaaactcgt ttttcttaag ttattgactc tatgtgattt 2100
tgctcttaag aaatacttac cgaccccttt gtgtttttcc tctcaaaatt ctataggcgg 2160
tctctgtagt ttttttcgtt aacaatgaac aacctaaaat ttgcatgtct ctgtcgaggc 2220
attccttcga aaaatctttg aaactcgaag attttttgtt tctctttttc tcttgattta 2280
gttcctactg attcgtgttg tataccctcc cttgtgatat ttacttataa ataagtcggt 2340
tgtgtacagt attttttgtc attgttacaa actgaaacta gtcaatttga ctataaaatg 2400
ttaagtgata aattctcgaa tgtgactttg aattaaggta gcactcacta tgtctaccta 2460
aaagatgatc ctctgtagtt atctatgaag aactagtagt taagtgattg acgttactgc 2520
attttttcta tcgtcagacg ctatttgtat ttttcttttc acacgaaaag aaagaccatt 2580
tagatgtatt tcctacttgt tttgattgca tctcgagaga ttcctctctt tgtatcagtt 2640
ctttagaacg tatcaaaacc taaaggctga catcttctgc caatattggt aatttctcgc 2700
tatgcttgat agctaaacaa acacttcatg tattcatcct atataacgat tttgacaata 2760
cgaatttatt ttggaacagc cctcaactta ttggttcatt gactattttc aataaaagac 2820
gtcagattca aaaaaaa 2837
<210> 194
<211> 3780
<212> DNA
<213> 筛豆龟蝽
<400> 194
ttttgcctga cgttagagtg tgtgttgtgt tttttttatt ttctttagtc gctgttatat 60
tttctatttt tgcatttatt ttaatttttt ttgttctggc acctcttatt taaacaataa 120
cctgtatatt ttattagttg tcagttaatt ttttttgatc tctgtaacgt ttagtttacc 180
gtaatactag atattcattt gtctgccgcc atattattta ggtcattttt tcttaacaag 240
aaataagtga aaatgaatct tgacaactat ttaaacttga aggtatttta gaattttttg 300
atctgaggtg acaaggacaa gaaccccctt cttctacttg aaagtttttt tacaagttag 360
aatttttgag tacaaaaatg gtgttggaga acatcaccga agaaaacaat agtaaagaaa 420
gcctggaaac accgtccacc atccttaatc ttaatgcccc aaagcatggg actattatac 480
caaatagagt gttcgttggt gggattgctc ctgctacaac tgaaatggat ttaatccatc 540
ttttctctag ttacgggaat gtaacaggaa caaaaataat ttcagatagg gcaggagttt 600
ctaaaggtta cgggtttgta acatttgaaa cagaggatga agctaaacgg cttcaaaaag 660
atgctgaaaa catagtttta ggacaaagac gacttaatat tgccccagct ataaagaaac 720
agggtttcag tcgagggaat tttgactgct cgactaatag tcctctccca ccaacacagc 780
tatatcgcca caatggtttt acctatgcat ttcacaacgg gatggcattt tttcagcagc 840
catcgcctac agtccccaca tcacctcatg tggagccttc tccagttttt cagactggtt 900
cttcatatgg tgcagtagca ccgagcagtc cagcagggcc agcttaccct tttccattcg 960
ctcctcaagg acaaattttc tatcaaacac ctcaatacca gtaccaccta cctcagtttg 1020
atggatgtta tgatgctgga cagatgatgg taacagatgg gagtttagtg atcccccagt 1080
actatctagc acctcaacca ccaccagaac ttgcatttta tcctcctacc cttcacaatc 1140
ctgaaacaac agcaccaata caacaggtga tttatacatc acctcgagcc tatgattcat 1200
ctttaatgta ttctacagag ccaaagtgcg gaatggagag cagcgtagga gctcacagtg 1260
gcgttagtat ggctgtggtc agtaaggagg agggagaacc accaacatcc aaccatcaga 1320
gacgaccacg tgaatcttcc accccagtgg tttccctcct caagctgtac aaagggaagg 1380
atgaccattc aaaaaagaaa cagcataaca cccatgatga gggaagaaga caatattacc 1440
tcaataacaa taatcctgat accaccaatc ggaacctaaa acaccaggaa tcctccagac 1500
atggaaacct tccctcccta ccttcttatc ctcctgtaca tactagtcct aaccttaagc 1560
accctactgt acaggttccc cagtttccct tctactccag acaggtgctc gccccgccag 1620
cgaccctagc atccttctat ccacccccaa ttccgcatag caggcctagg tccaactaca 1680
ggccaagata tggtggtggg agaggtggag gccgtggaag tggcaataag tggctaccca 1740
aagaagagga aatggggcag cctttgacac cgcccataac ccctcgttcg gagccctctg 1800
gtgtcactgg agccactact ggacctgaag atgtcgtcca gtctattcaa gctctagcaa 1860
tctagtccta gacactattt gtcttaaacc ataccaaata aggaagagaa ccttcgcctg 1920
ttataataat aacaattgaa gaaaaaaaaa taacaaaaaa aaactgaata gtcatttgtt 1980
taagagaata tatatacatt tgtttatgta tataaaatgt atatttataa ataaatttaa 2040
agcataattt gatatttttc tctatgatgt ttaaaacatt ggaattttag ttcttaaaac 2100
acaacacacg ataatttgtt agattctagt ccaggaagga ctcatcgaga tttgagactg 2160
tttgatataa tttgtaatta ttataatgtg acatttcact tgttaactta aatgcacatt 2220
atttgataaa tagtgccatt tatttttact gtatggatgt ttgtttaatt cttttcactt 2280
gcttagactt tatcagactt ttctacttct gctaaagatg agatcattct aaagatatta 2340
aattgaaatg catcatttta tctgaaatat agatcagttt taatcttcat ttgtttgtgg 2400
taagctttta cataaatgtt taaagttctg ttattacaac ctcgaaacaa aattctctag 2460
atggccaaat caaaactttt gtacctgtta aacttcttta ataagttagt ttaattttta 2520
taaaagacat ggtcttcaga taggtaggtg tacttaaaac ataagacaga gtatccagat 2580
ttttgaatgc atttctggtc tttgtgataa tttagtccaa agctgagaat gtagtaattg 2640
tataaaatat tagtatatat ttaaatatat ataaaattta atatattttg gttaatatgt 2700
atgttatata tttattgtac tttaatgtaa gtgttaatgt taagtagttt ctagatctgg 2760
ttatagttaa ttttttttga aatcgctaag atatgtttaa ggttgagatt agatacaata 2820
caataaactt gcatttattt taccctttaa aaatttattc tatattaata tcaaaattgt 2880
gctctgttca tcaggatagt ttgtaattgt tagactgtaa gacgactttt gattgattaa 2940
tcaattgtaa aattttagtt gttgctcata ttccattatt catattataa cttgataata 3000
atatttttta taggttaaat gttagatatt aaggttcatt caattagtta catgtctggt 3060
aattgtcttc cttaagtttt caattttcag atttgttttt taagttccta catttttaag 3120
aaacccttta gtattcgttg cttaaaagca caagtatcct taactatggt ttcataccta 3180
cttctgaaaa gataaaattt tttttctttt ctgtggctta ggtgtaagac aactagtcaa 3240
acacaaaatt tattcataaa aaaaagaaaa aaaggatttg ttaatgtaag attggacaag 3300
attttgttat accaataaga taaaactgtt gagatgagat ggactatttg ttcatgtatt 3360
ttcgatgtat attataaaag attttgttac atcaaaatga taagtacgat ttgagcagtt 3420
agaagaatag ttatttttta aaaatataaa tacaacaaaa atcatcagca tagcatatat 3480
ttataattaa aaccactact tcaggctgtg atttttattg taatacattg tgttagtaat 3540
gatgagttta atcttaaaaa atgtaagttt atttatgatt ataacttaat tactttccac 3600
cagttaagtg tataagttac aagaaaatat ttgtaccata ttccaaggct gatgataatc 3660
gccatactgc catgaaagtg tgttgtactt atagattttt tttttataga accacttgta 3720
ttcaatttac tatttgaaaa taattgcctt atattctact aaattctttc agataaaaaa 3780
<210> 195
<211> 2816
<212> DNA
<213> 褐臭蝽
<400> 195
tggatggtgg agatggcctg gcgttagagt gtgtgttgtg ttttttttat tttctttagt 60
cgttgttata ttttctactt atgcatttat ttttattttt gtgtgtccgc gcctcttatt 120
taaactagtt tccgtagatt tgttaagttg tcacttaatt tttttaatga tctctgtaac 180
gcttagttat ctgtaataat agatgttcat ttgtctgccg ccatattatt taggtcattt 240
tttcttaacc gaaataagtg aaaatgaatt gtaagaactg tttaaacttg aaggtatttt 300
agaattttga tctgaggtgt gacacttgtg gtgaacattt cttaataatt taaaaggcag 360
aattgagtga atctcttact gctttataat ggctgtacaa ctaaaaaatc aaaaaaattt 420
agcagctttg agtgaagaaa aggatataaa taaagatcac atagagtccg caacttcaaa 480
tataagtaat aatttaaatg caccaaaaca tggaactgtt attcctaaca gagtgtttgt 540
gggtgggatt gcacctgcca caactgaatt agatttaatc caactattct ccagttatgg 600
caatgtcact gggacgaaaa taatatctga ccgcgcagga gtttctaaag gatatggttt 660
tgttactttt gaaacagaag atgaagccaa gcgtctacaa aaggatgctg agaacattgt 720
tttaggacaa cggaggttaa atatagcgcc tgccataaag aaacagagct ttggtcgagg 780
cggttatgat tgtactgcag gtagcccaat acctcctact caagtctacc gccacaatgg 840
tataacattt acttttcaca acggaatggc attttttcca cagccttctc ctccagtccc 900
tccatcatca catgtcgagc cttcacctgt atttcaatca ggtgcatcct atggagctgt 960
aactcctagc agtcctggac ctgcatatcc ttttccatat gcacctcaag ggcagatatt 1020
ttatcaagct ccacaatatc aataccatct tcctcaatat gatggatgct atgaaggagg 1080
gcaggtaatg gttactgatg ggagcctcgt tatccctcag tactatctgg cagcagctcc 1140
tcaaccaaca cctgaattgt tctatcaaac tgctgcaatt catccacagg aggcatctcc 1200
tcagtctgtg cccccgttat tatatgcctc ccctcgggcc tatgattccg gacttgttta 1260
ctccacagag cctaaatgtg gattggagaa tggtatgagc aatcataatg gcggtggtat 1320
ggctgtgata aataaggagg agggagaacc accagtcact aacaaccaca ggcgaacacg 1380
tgagtcggcc cctgtggtct ccctcctcaa gctgtacaaa ggaaaggagg accagcctcg 1440
gaggaaacaa caccatcagc aggaggaggg gaggagacag catcatttga acaataataa 1500
tccagatgct gccaacagaa acctaaaaca ccaacacgat actaggcgtg gcactccccc 1560
ctctgttcct ccatatcctc agaaccatgc tagtcctaac cctagacacc ctcctgtgca 1620
ggttccccag ttcccgtact atccccgcca ggtcctcgcc cctccaacag ccctcacgtc 1680
tttctaccca cccccgccac cgcctcctag gcctagatca tactacaggc cgagatatgg 1740
aggaggaaga ggtggtagcc gaggaggggg gaacgccaag tggttgccga aagaagaaga 1800
gatggggcag ccattgacac cacctataac accacgttct gaggcagctg ctgttactgg 1860
cactacgact ggacctgaag atgttgtcca gtcaattcaa gcacttgcaa tctagttcag 1920
cagttgcctg gccggacagt ataagacaac atatgtctat atacctcacc gtaacagata 1980
accttcatcc tacatcgtct tataacaaca ttgaagaaaa aaaaaaccaa aaaaaactaa 2040
aatagtcctt tgttttctct taaatgcaag agaatataca taaacttgtt tataaatata 2100
tacacataat atatgtaaaa taaatttgaa aacaatattt gtttgttttc tgttatgact 2160
taaatttttt aagttaaagg atagcacaca aataatttgt taattctagt ccaggaataa 2220
atgggactta ttgtgagaaa agagactgtt tgatataatt gtaaatatta tatatgtgac 2280
gttcacttgt taacttaagc acattgttat ataaatagtg ccattttttt ttttactgta 2340
tggatcttga attatttcac caattagact ttatcagact ttttctaact tgaacgaaaa 2400
cattattgct caaaattcgt gataaaattt tatgaaagta gaattttgga attatgaagc 2460
atctcattag aacgttgtag tctcatctag ataaatcagt tttaatctcc atttgaaaaa 2520
tttttgtcaa gattttcttg ataaattgag atcttaaagt ccaaaagtgt aacagttata 2580
aaatggcaac gattagcact tgtacctgtt aacgcgttgg ggtttagtta gtttaatttt 2640
tataaaagac actatcttga aaagatgtac ttattatgtt tggaatttgt atggaagtcc 2700
gagttagcag ttttttaaag tgcatttctg gtctttgtga taatacaata tcagaagtaa 2760
gttaaataaa tgtgtatttg tgtaaaataa tataaatata tatttagtta tatata 2816
<210> 196
<211> 2823
<212> DNA
<213> 稻绿蝽
<400> 196
ggtgcttaag cctgacgtta gagtgtgtgt tgtgtttttt ttattttctt tagtcgttat 60
attttctact tatgcattta tttttatttt tgtgtgtccg cgcctcttgt ttaaactagt 120
ttccgtattt ttgttatgtt gtcacttaat ttttttaaag atttttgtaa cgcttagtta 180
tctgtaataa tagatgttca tttgtctgcc gccatattat ttaggtcatt ttttcttaac 240
cgaaataagt gaaaatgaat tgtaagaact gtttaaactt gaaggtattt tagaattttg 300
atctgaggtg aaattgttgg taaagatact tggaaattta taaggcagaa gtgagtgaat 360
ctcttactgc ttaatatggc tgctccaata aaaaatcaaa aaaatttggc agcattaaat 420
gatgataaag aactcatcaa caaagaacac actgatacag catcaaatat cagtagtaac 480
catttaaatg ccccaaaaca tgggactgtg attcctaaca gagtgtttgt tggtggaatt 540
tcaccttcaa caaccgaatt agatttagtt catttatttt ccagctatgg aaatgttact 600
ggcactaaaa taatttctga tcgtgcagga gtttctaaag gatacgggtt tgttacgttc 660
gagaccgaag aggaagccaa acgtttacaa aaggatgcag aaaatatcgt attaggacag 720
cgacggctga atatagcacc tgccattaag aaacagagct tcggtcgcag tacctatgat 780
tgttcagcgg gtggtccagt atctccttct caactgtacc gtcaaaatgg cgttacatat 840
gcttttcaca acggtatggc tttttttcaa caaccaactc catcattacc tccatcagcc 900
catattgaac atatgccagt cttccaatca ggtccgccat atggagcgat gacgcctagc 960
agtcctggac cagcataccc atttccgtat gcgccgcaag gacaactgtt ttatcaagct 1020
ccacagtatc aatatcatgt tcctcaatat gaaggctatt acgaagcaag ccaggtgatg 1080
gttactggat tatataattg tgattttcag caatatgaag gctgttacga agcaagccag 1140
gtgatggtta ctgacggaag cctggttatt cctcagtatt acctgacaca tcaaccaaca 1200
cctgaattgg ctttttacca gccagctaca gtgcacccgc aagaggcatc accacaatct 1260
gtgccacctt tgttatatgc ttcacctagg acttatgagt ctgctattat ctattccacg 1320
gagccaaaat gtggattgga gaatggtatg atcaatcata atggaggtgg tatggctgtg 1380
ataaataagg aggagggaga accaccagcc caaaacccga ggcgaacccg tgaggcagcc 1440
cctgtggttt cctacgttaa aatgagtaaa ggaaaggagg accagcctcg aaggaaacat 1500
caccaccaac acgacgaggg gaggaggcag catcatcatt tgaacaataa taatccagat 1560
gctgctaaca ggaacttgaa acatgatgca aggcgtggca ctcccccctc tgttccccca 1620
tttccagcca accatacttg tcctaaccca agacaccctc ctgtacaggt tcctcagttc 1680
ccgtactatc cccgccaggt cctcgcccct cctacagccc tcacgtcttt ctacccaccc 1740
ccaccaccac ctgcccggcc aaggtcatac tacaggccga gatatggagg aggaagaggt 1800
gggagcagag gaggaggaaa tgccaagtgg ttgcctgttc ctaaagaaga agagctgggg 1860
ttgtcccagg cactgacacc acccgtaaca ccccgtaatc ctgatacagc tgctgttact 1920
ggcactacga atggacctga cgacgtcgtc cagtcgatcc aaggactttc actctagtct 1980
agagctgttg cctggccgga cagtataaga cacgatatgt ctatatacct cacatcagat 2040
aaccttcatt ctgcaactat caacttgatc tcattgaaga aaaaaacccc aaaaaaaact 2100
aaaatagtcc tttgttttct cttaaatgca agagaatctg cataaacttg tttataaata 2160
tatacacata atatatgtaa aataaatttg aaaacaataa ctgcttgttt tctgttatga 2220
ctttaaattt ttaagctcaa acacaacaca ccaaatattt gtgaaatcta gtccaataca 2280
ggactcattg agaatagaga ctgtttgata taattgtaaa tattatatat atgacgttta 2340
cctattaact taagcacatt gttgtaaaat agtgccattt tttttttact gtatggatct 2400
tgaattattt cactatttag actttatcag actttttcta ccttaaacga aaacattatt 2460
gctcaaaatt ggtgataaaa ttttatgaaa gtagaatttt gggtttataa aggtttatag 2520
tttctcaata gaacactgta gtctcatata gataattcag ttttaatctc catttgaatt 2580
ttttgcaatc caaaagtgta acagttataa aatggcaagg attaacactt gtacctgtta 2640
acgctattgg aattggctag ttaaattgtt ttaaaagaca ttatcttgaa aagaaagatg 2700
tacttatact gaaatttgta tgaatgagtt ggcagttgtt taagtgcatt tctggtcttt 2760
gtgataatac cagtctatta aagtatctaa taaatgtact tatactgaaa tttgtatgaa 2820
tga 2823
<210> 197
<211> 2006
<212> DNA
<213> 玉米穗虫
<400> 197
caactgttga cctagttccc tcgcctagta aaaatagcgg tcgacaatag ctcaaacact 60
atttatagtg cgtatctaaa aaaatctcaa atcacacact gttatctcgg ttgttttttt 120
tcgaaacatc actcgaatac cgtccgtgtg tcagtcacgt cgtggtccgt acggcgcaag 180
tctggagccc acctatacaa cacaagtgct accgtaatcg ataaaacctc gcgtgaaaga 240
cagtcagttg ttttttcgtg aataccagtg cccttttagt tgccgctaga gattttagtt 300
tagtaaacaa gggaacatgt tatattagtg ttttaaagtg ttagaaacaa tggaaagtgt 360
taagaggtac ataaggagtg agtctgcgag ttgcgctcat atggttgaag aaagcacggc 420
gaataacagg aagacggcag acccccacga gaagctgaag gctttgttga actcccctga 480
tgacgatacg gacgacacct tgcctccatc agatgctcct aaattcggga cagttataca 540
caatagaatt ttcgtgggag gcttctcttt gaccacgact gatgaggacc tgtggaagtt 600
cttctcaggg ttcgcgacgg tcacagcagc cagggtgatc tacgacagag ctggcgtctc 660
caagtgttac ggcttcgtca cattcgccag ccccagggtc gcgaggctga tcgttaaaca 720
gagcggggga gtgatgttct cgccactggg gcgactgcgg gtggcacagg ctgtcaggaa 780
acagatgagc caactcccag tgctaggctc agaggcagcc ccgctccagg ctccactttg 840
ctaccagctg ctgtgtgctc cacccctcgc gcctctgcag ccctgcgcgc catgcgagtt 900
gcctccacca ccgtatgctg tgtacccgct gccgtttgac acaaccccag cgatctacgg 960
tcccccagct cagtacgcgc tggtgccagc tccatacagc actccatgct gtgagccctc 1020
atgctgtgca cccctcgaca agcagccccg ggtgccgccg ccaccgttac accccgcgtt 1080
catttactag actcaccccc caagaaatat attaaaggca ttctcgttta gatttggaag 1140
tacttaaaaa ttactactga tggtggattt aaggtatatt tgtgagagaa tgaactattt 1200
attggaagta aaacacgttc taataacagt atggaacata ataacaactg tatggtataa 1260
agcgcattgt acatggaatc gtgagtacag atgctgtgaa ttttttaatg gaatgtacag 1320
ctgtaacaaa gtatacaaaa agccagcaaa agataccgca agatacgaaa ctttaacaag 1380
ataaggccac aacagcaatg catcttgtag ataacgtagt atttgtcaaa atattaacat 1440
tctctatatg aaataagatt ttttccactg tcacgataat atctcctaaa aatactatca 1500
ataattctta tagccaatgt ctttgatata tttctgctca taacatcatt cgcgaagaag 1560
gtttatttac aaattgttca gcttcttcga tgtattccat cttgattgct caagtcttcc 1620
aatccagttc atgctcatct tcaaacataa ctcctctatg aataaactgt ctgtgagttt 1680
gtttgtgact aatctacgga catccagctg cgatggtgta agtgttcaag ttgtcacgtt 1740
tgtttacatt tctctgattg tctggtcgct tttatttata gacgtaagta cctatattta 1800
gcttcaacct ggtatattta agaaaccgtg gattcacgtg ctatcattat tattatagtg 1860
aattttattg gtgtatcaaa ttgctattta aatcagataa ccttgatcat tccgtgtcgt 1920
gactttcaag tttagataac aataattatg tcatccacat acctacaacg atctaaatgt 1980
gacatgcttg tatctgtgtt aaattt 2006
<210> 198
<211> 6665
<212> DNA
<213> 欧洲玉米螟
<400> 198
agtgcgtatc tttaaatctg aaagcataca gtcttatctg gttgttgttt cggcgacaga 60
tgtggagtgc gtaaggccgc tccgcgcacc catgtacacc tcgcaaaatc gatagctcag 120
cgtccgtctc agtgtcgact tgttttccat cgaatttagt gctcgcttag tccgctagcg 180
tttttgttag aagtatcatg ttaaattaat aatggaatcg tcagtaaaga ggtacattag 240
gagtgattca gcgaactgcg cgcatatggt tgaggagagt gcagcgagtt gtagaaaagt 300
ggtggatccc cacgagaagt tgaaagcgct gctgaactcg cctgaggagg acacggacga 360
ctcgttgccg ccgacggatg ccccgaagtt cgggactgtg atacataata gaatttttgt 420
ggggggtttc tcgttgacga cgactgatga agacctgtgg aagttcttct ctgggttctc 480
gacggtgacg gcggcgaggg tgatctacga cagagctgga gtctccaagt gctacgggtt 540
cgtgacgttc gcgagtcccc gggtcgccag gctgatcgtg aaacagagcg gcggagtcat 600
gttctccccg ttgggacgtc tccgagtagc acaggcggtc agaaagcaga tgacccagtt 660
accagtgctg gactcgggag cggcgccgct acaagcgccg ctgtgctacc agctgctatg 720
tgcaccgccg cttgcgcctc tgccaacctg tgcgccctgc gagctgccgc ccccgccata 780
tgctgtgtac cctctgccat ttgacaccac tccagcaata tacggccccc cagcgcagta 840
cacactagtc ccggctccct actcggctcc ctgctgcgag ccagcttgct gcgcacccct 900
cgacaagcag ccccgtatcc cgccgccacc actgcacccc gcgttcatct actaggccag 960
ggccattagt ttccagaagt gtgtcttctg aaagcctggc tctgggagct ggtaaggttt 1020
ttgctttgga atttccaggt tttgatgaaa tcggcaataa agtaaacttt tcaggaggct 1080
ttgattcctt tgaagatgaa gaaatgcgac tgcaaaggat aaggttgatg atgaaagtta 1140
tgatttcgtc gatagcaaag tgaagttttc aagaggcttt gattcgttgg acgtaaagga 1200
aaagtaaccg aatgcaaagg ataggattgt tgatgaatga attgttaagg aagtagacta 1260
attaaaagca ataaattctc aggttaagta gattggaaga agtaatgcta aggaagtaaa 1320
ggacaaaatg cctctcgaaa gttcttggaa attcaggaaa ttagcactca agccttgtaa 1380
catcaatgct cccagaaccc agaacctttt ggttagttct caagtagacc agtttcctgt 1440
tcattatgcg ttttatatct atcgcataat gttccagtca ctggctttga tatttgagaa 1500
cgatgacgta cacttttgga aactaatcta agtagatatt taagtgtgta tttaggggat 1560
catcgtcaga aactagaact ggttggtgca gtgagagcat cctcgttaat tttatcagaa 1620
gacgaataaa aatgacgtga attttaacgt aatcgatggg aaattttcga tcgtctgcaa 1680
gcttcctaaa taccccaatt ccatattgtc tatcaccact cccccattgg taattaaaca 1740
tggttcctct ccttaaggtt tggtttctta ggttagtttc atttgaaggt tgttcccaaa 1800
ttgttccttt tgctcaagtc ttccaacttt atcatgctca agttgtttca cttccattat 1860
ttcaagccat tgtaatcagg cgattttgtt gtggtattag agtaaaacta gctgtactat 1920
taaattttgc tgaatttgaa ataatgatag aggaaactta ttcgaaagaa atatcaggtg 1980
atctgtattc ttcttataat gatgagccta aatcctggtg ttttatcatc ttgctaatga 2040
agtgtgaaat atttcaatga agtttatatc atcatcatca ccaacagccc tttatagtcc 2100
actgctggac tcctttagtg gaggaaagtt acttgatgtt attcaataca aaggtgtccc 2160
taatgccaca agaaaatcgc ctgaacaaat tcctccatta ccctatttat agaatttcga 2220
attgattggg aaacgtgaaa gatctggtgt tgaaagtgtt catggtaggt ataactttct 2280
ggtgtgtttt gaccgttttg tctcctgtac gtagtctggt tgcttttatt tttagactta 2340
agtgttagct tggacaatcg gtgtaaaaac tgtgggttcg tgtgctatca gtaccatagt 2400
gtgtaatata atagatatat aaaatcattt taacttcttt acttgacgga aattttctat 2460
ttgcaagaga attttgtctg tcaccatgtg cagagttgtt cttagcaagt ccatgtacca 2520
caagataatt aatttctaaa cttgatttga aattcaaaaa tcattgaaga aattggtcat 2580
tttgaagtag aattttgtgg caaaagaaaa catgaagtga ttttatgtat ctatgaaaga 2640
tgggtgttag ataataaagt gcaaaatgag cacaacgatt catagcacaa aacggctaag 2700
ttgctagatg tagaagacgt tacctccgtg gaaaaagaca tatttgttgt attagcttgt 2760
tatccttgaa aaggagtttc gtttacctta gtgtaaggtt gttttcgaaa gcaattgaca 2820
tagacagaga tataaaccta ttttttaata aatttagttt ttacatgttg ttgattgtca 2880
ctttattaaa tctggggtcc aaatgtgaag gcatgtaaac attgaaactt atttctttta 2940
gcagatgtct gtggtgtatt tgatgtttat atttctacac atgaatgttt gttgaaaaga 3000
cgacgataag aactttgttt cagatataca atagttgttg cagtgttaaa taatcctttt 3060
gacgttgtta tttgcttgtt tggtaaataa atagatgacc aggatgttat agttctcata 3120
agactaattg caaagtcagg aacgtttatt aatatgatta gttatatcga attctattaa 3180
aaatgttaca ctgtcatgtc ttccttgttt ttctgagctt catcgttatt taccaaacaa 3240
catggtttat tttatagaca gtttgatggc taccttacgt taggtgtaat gttagttgtt 3300
attacgttgt ccacaagttt tgatggatcg aagtacacaa atcgtcaacg atgtgatatg 3360
ggatattcaa taacgtagca ttagttttat ttgttagaag acttaatagt tttagattca 3420
acacattttt aaataacgtc gtttgtctat cactcggaaa aataggacca aagacattcg 3480
cagaagcgtg caaatggtca cctgtagtag tgagtcacaa agtctcttaa tacgtaaatt 3540
cgttgtaagt gggtgttgat aatttttata ttcgtcactt ttagagctct ttaatagagt 3600
ttcctgtaga cctagagagc cgatgctctt caacatttcc aaattgtgtt gtattattaa 3660
aattttatca cgagaactag cttgctcgtc caagcaataa attcttgctc tgcccttagt 3720
aataaggctt atctcaatta ttatattaca tcatatacga ttagtattac tggaaatgcg 3780
gtcaatttgt cgttttggat ttttatgact attattgtta gtactaagtc tagtatattg 3840
tgtaatcttg cgatgctttt gtgctcaagg tgttatcttt tttttttgtt ttatttattt 3900
ggtacacttt tcgcaacttg ggctatgttt tcacagcgct tacaattaaa gctttctttt 3960
cagcttttaa tgtatgctag tacttggcta gtaagctttg ctttaggaac gacactggag 4020
catttggtaa gtgtgattga atgtacacac tcaaaaatag tcgcatctga tcttgcattg 4080
atttttctct ggccagcatg agatggtatt gccgtatcct ctaaaataaa ttttatctta 4140
attatcacag tggaaatcta agtgcttggc caggttgcac agacgggtgc atagcgtcaa 4200
acatataacg cgtacgggcg ccattatcga cactcgatta agatttcata atatgtttga 4260
gtacaacaaa gcgatgcgcc tttgtgtgca gcctggtcaa gcaccataag tagtactatt 4320
ttatcgtata gttaaatcgt cgttttatta tttcaaagca aagtgaaagc atagccatta 4380
attattgtgg tcgtataact atcatgatgt tatggatgtt tcggcatgtt ctgttgatgc 4440
aaagtgttgt gaactcgtag tctcttctgt gctatgtgaa gtacaagata ttttatatgc 4500
gagctattgt aggttattat taagcatgaa actctatcta tcacttatcg ctgcttggtc 4560
tctatcatga atatgtatgt ctatttcttt atgtggtatc aatagcaaaa tttgtaggac 4620
aaacaaaact ttttcatttt attctgtcta aactgccaag tttatattat tatcttttct 4680
cttcaacttt tttctactat ttctttaaat attattcaac ttaaagtacc tagtaatact 4740
cattattgtt acaagcatta tagtagtaaa gtttccgtat taaatggaaa aaaatatcaa 4800
tttcgtgtat tgatggcatg ataagagttt gttataggta atttatgatt ctagctacaa 4860
taataataat tatgcaagat atagaatttt taagtaatta ttttgatgtt tttttttata 4920
gtattttata tcagtaaaaa catatgtatt catggtattg gccttttcta ccaatattgc 4980
gtggcctagt gacaactgcc tgacatatcg gataattaat cactatgcta tagtatatta 5040
ttttttgtat aacttgtctt gtatataatc tctcgctaga tatattagat tcgtgaaatt 5100
aaaatgatgt aatttagaca atattactct aaactatatt aggaaaaata ctaaggtaag 5160
taatatagtg taatctatgt ctatattctg tgtaagtgga tcactcctat atcttttagc 5220
aaaggcttag aaaacgttgt gattcagaag ttttgaatat ctaaatgttg ctaaattgtg 5280
taagaaatat aaaagtatat tagggaagct accctcctca gatatcgcta gattagcagt 5340
aaaattttgc agacacgtct ttaataaaat atagcttaaa ctgtcattta tattatatta 5400
tcattaataa tatatttgtt cgtactttat attattttct agctgaaact gttaataata 5460
ttcaaatttt catataggta atatagccgt tcaggacttg aaacaaaaac atttttttaa 5520
tccgtttacc aaagtacttg aacaaaaaaa gcagcagtca caccagcaaa tgtcaccttt 5580
gattatattt ttgttatttc atagaatata tctaaatccg tctgaatttc cgctgattta 5640
tttatactta tatgtatata cagggacgat gacaatatac aaaagttgtt aagaaaaaat 5700
aaactttttg agtagttggt aatcctgaga agaatgaacg tccaatttgt ttttgtttac 5760
atttcgcaaa ggcagaatgt atgctgttta attgtaatgt tgttaccatg ttgatatcaa 5820
atatcaacag ctgtgtgtta tgtcatccgt taaaaatagc atcattatta gcaagaataa 5880
taaatcataa tttaacaata ttatttagtt gattaataaa ttatattatg gttgtcacct 5940
aaaaggctta aaatgactac tttttcagta gttaattaat ttacaaattg taaaacaaaa 6000
aagcaatcga aaaaaactta acattcatgt tgttgttcaa attctatttc taaatctcga 6060
tggtataatt attaaggtgg atacatactt tgtgaaacga acctacatta ttatttcagc 6120
aaatgcttca caatcaaaat gcctcagtgt gagctgtgca taactatgca gtagtagagt 6180
agctgttcag catgctcttt ggaacatatt tacgtgaatg acatacaggt agatagattg 6240
tatttgcttg cttttcacgc aaaggataat gctggtatgc ttacaaaagc acctttaaca 6300
gaggcattcg ttttacaccg tgtacggtgt atgcctcaaa aagtctgtat ctacctttat 6360
atgcactttg cttcggcggg tagtctacat gggagcttcc ttatttctat acaacgactt 6420
gtaatataac aaaagctttt gaaatattct aaactacgcc taccagaaat tatctattta 6480
actagtcaaa ctctatttaa ttttattcaa accaagaagc tgtcacgcat ttgtctaaag 6540
cgttaccgct atttagttat gaagagttca taattattgt tgtacttaat aatgagttgc 6600
taactaatta actgcgtact taaataaaaa tatatcaaaa taatatttat attttttgtc 6660
caggt 6665
<210> 199
<211> 6032
<212> DNA
<213> 草地贪夜蛾
<400> 199
gtcaaccgct gacctagttc cctcgcctag taaaaatagc ggtcgacaat agctcaaaca 60
ctatttatag tgcgtatcta aaaaaatctt aaatcacaca ctgttatctc gcttgttttt 120
tttttaaaca tcactcgaat accgtccgtg tgtcagtcac gtcgtggccc gtaacgcgca 180
actcccaaaa cctacctata caacataagt gccaccgtaa tcgataaaac ctcgcgttac 240
agacaatcag ttgttttttc gtgaatacca gtgccctttt agttgccgct agagatttta 300
gtttagtaaa caagggaaca tgttatatta gtgttttaaa gtgttagata caatggaaag 360
tgttaagagg tacataagga gtgagtctgc gagttgcgct catatggttg aagaaagcac 420
tgcgaataac aggaagacgg cagacccgca cgagaagctg aaggcgctgc tgaactcccc 480
cgaggatgac acggacgaca ccctgcctcc gtcagacgcc cccaagttcg ggacagtcat 540
acacaataga atcttcgtgg gaggcttctc tctgaccacg actgacgagg acctgtggaa 600
gttcttctca ggtttcgcga ccgtcacagc cgccagggtg atctacgacc gagctggtgt 660
ctccaagtgt tatggtttcg tcacattcgc cagccccaga gtcgcgaggc tgattgtcaa 720
acagagtggt ggagtgatgt tctcgccgct gggacgactt cgggtggcgc aggctgtcag 780
gaaacagatg agccagctcc cggtgctggg tacagaagca gctcccctgc aggctccgct 840
ctgctaccag ctgctgtgcg cccctcccct ggcaccactc cagccctgcg ccccctgcga 900
gctgcccccg cccccatacg ccgtctatcc cctgccgttt gacaccacac cagcgatcta 960
cggcccccca gcccagtacg ccctggtacc agctccgtac agcaccccgt gctgcgagcc 1020
ctcatgctgt gcccccctgg acaagcagcc cagggtacca ccgccgccgc tgcaccctgc 1080
attcatctac taaactcact cactcacgaa atatctcgaa gacattcaaa ttcaaggtaa 1140
ccatttcata ctagatgtcg agtaagcgac aaaaatgtat tgtgaagcta atatagcaaa 1200
aatcgaacag aacatagttg aaattagtaa aacagataga gttaatatgt gtaccaatga 1260
tgtaactagt caggtgtaag tcaaaacaat aatggcaata attgtggaca acgaatgtaa 1320
tgcactaacg taagtactga gttttgtttg tagcactgat cgtgtacata gatgtaatgg 1380
gatgacgcgc tgataacgac ggagtaccga gcgatctaaa gataaagcga gatatcaact 1440
cgaagcaaga taagacgaga gcgatgcagc tccttacaaa tacatttcac cacgtctgtt 1500
tgttctatct cgttcctctc ataaataatt catatttcaa tgtcttcgat atatttccac 1560
acgacaccat tcgcgaagaa gattacttta ctaattatat tcagcttctt cgtatgtatt 1620
ccatcttgat tgctcaagtc ttccaatcca gttcatagtc atctgtcttc aatacattac 1680
ttccctataa tgaaactatt tgtgagagat tgtctgtgac tattcaatgg acatcaaagc 1740
tgagagaagg cgatgtatgt gttcaagtta gctctcgggt ttgtttagat ttttctgaat 1800
gtctgtcttg tcgcttttat ttatagattt aagatattta gcttgaaact ggtatattta 1860
agagaccgtg gattcgtgtg ctatggttat tattatagtg aattatatcg atgtatcaaa 1920
ttgatgtttg aataaaagtc gtgcgatgtc ccgtggaaat actttaacca caaatattta 1980
atgtcatctg catgtataaa tccaattatc ataatgtgta tattcatata tctatttaca 2040
cagtcttgta tttgaatgcg aaaagtaatt caacattttt atcaaagtta tcgctctata 2100
gtaatattgt aacgcgccca agggaaatca atttgataca tctttagttt ttctcatgat 2160
aagtgtgaaa ataccgaata taaaataact ctagtgcgga tatttctaca aaatatttaa 2220
ctgaatcata tttacagaat gtatgttaga gcagtccaca gcctcggtct aagtcattgt 2280
aataatgttc acgtaatgta tgctaatgaa attattcaaa tatgtaccgt acctagtttc 2340
catatatccg cactcgagtc aagtatttac aaacacagtt caaaaatgtg cgttataagc 2400
acaacgaatt aagcacaaaa tcgaatagca gctagtttta gatgacgtta cctctgataa 2460
aagacagatt tgttattgct tgttatcctt gaaaaggatt tttgtttatc cttagtgtaa 2520
gattgttttg tgaaacccca ctgttggttg gcggacgtct tgggggtggt gggagacacg 2580
attttgatag aagacaattt gtagaataat cataacttgt aattgagtaa gaagatattg 2640
gtaagagaga tatatcgctt gtacgagctg tagatgtgga ggtaggtaga caattagaga 2700
tcttatcggt tggctgcttg cctacttaac gagggaccta gtttgaagat aataatagta 2760
cttttagagc cagattttag aaccaacctc aagacgtcag acaacgcgtg actagaacag 2820
agagaaacaa aaatattttt taatattatt tagtttttac atgttgctgt tgaaatacac 2880
tggggtccaa tatgtgataa ggaaatgtga aattcaaagt tgaacgaaca ttttggaaat 2940
cacatttcta tacccgttat tgttgcgttg gaaataagaa tcgaatcagt ttttatgatg 3000
cacacattaa cttgctaaga cgtgacgtgt ttcagtaaga tctacttaaa gttaccggag 3060
aatgttgtca aagtatttat gaggaacgaa atgaagtata gatgttactg agacatgtta 3120
acgatgcaat cacgaatagt atttgttttg taattgttaa cattgattga acagccgctg 3180
ttcgcttcga agtgatcaac aatagacttg acaaacggat ccataaagca ataatgtcgt 3240
caagatgtga tatgggataa tttgttgact agcaatagtt ttatttagat acattagaga 3300
gtagattgtt ttaaaatcct tcattttacg gcccgttgca tcgtatttaa cataaatatt 3360
gaagaattta aataattatt gtataaggtc tctagcacat gtgataactt ccatgtcctc 3420
gttcttcgtc ctatggcgta tatgtggctt gtctaagcta gaatttcccc ctgatgcaat 3480
tgggttctca cttagtacca gattatttat aggaccaaac aaagattccc ttacatcacg 3540
actaacgcag agatcttcgc atctattctg tctagactag tatacctatc acaaatctct 3600
atacgtaaaa ttgttgtatt aatattaatc attttgatat tatattctat cactttttat 3660
ggccgctagt tgtgtgatgc tacggtgaat gaatttgcgt ttatatcaag gtcttccttg 3720
ttgtttttta tatcactggt taagacaact tcactgttat tgttattatt tattttcatg 3780
tttactgtcc cattgtttgt aggtgatggt tctttaagat caatttctag tactgtcgtg 3840
ttattacctc ataggtttga ggtaatgtct acatgattct tccactagtc agatcgacag 3900
attgtcagca gagttagcag tacctgttag ataagtaaag gttccattaa tttcttcgtc 3960
caaatgttaa gttgtttcta ctctatcagc aagttcattc acccaagcac gttcgatgtg 4020
gtcttctgcc tttagatgta aggctttcgt ctcaattact attgtacatc ataattatac 4080
gattagtgtt attgaaaatg ctgtcaattt atcgtcttgg atttttataa tgtatgtatt 4140
tattgttagt tgtaagtata gtacttatgc gatgcttcgc tcgagtatca tttatgtctg 4200
tatcttcgtg tgcttataac ttaagttcct tggatttgtg ttacctcaga ctaaagttta 4260
tatctaaaat attgttgtta tatcatattc tatctatgtt ggttcatgcg atgggagttt 4320
tattttgaat ttggggaaaa tgggccatgt ttgtggaatg tcgttattgt ccgaaacgtc 4380
aaaagatact aactttaatt caaggtcact gaaagtatga ttcgatctaa ttgaagattt 4440
taatcatgat caaggtttag agcagctgat gtgttcacac aaaatccgta acgaaatatg 4500
aaaatgtctt agagatggct gttgctaggg ttgcttcaat gttggtcagc tatgaccatt 4560
ttctccaaca tcaaatcatc gcgtgaacca gattagcact catcgtatac atttcgatgt 4620
tgcactatcg atgtttcgag tacaaggaat ctatatccaa gctaaactta agtctaataa 4680
ctatcacttt cattgcttct aaggtttctc acgtaaatac atattaagca ttcaaaaacg 4740
catttattaa gattgaaaca cccataaaaa attgtgaaaa atgtcttaaa ctgtgagttt 4800
ttgttccaaa ttatgaatta tacgactatg aagtgctcat atagcaattt tttgatgaaa 4860
gaaatgagtt gttttctgct gtagatatgt atttacgtga gaaacatact acgaacataa 4920
taagtggcct agagacatac tgcctaacat atcggataac gaatcactac gctatagtta 4980
gttatttttt ttataactgt atataatctc tcgctaggtg gtcatagttg tgtggacgaa 5040
tataatgtta gacaatatta atgtaattat aggttaggaa gattaacgtg tatttattga 5100
aattgtcact tatcttagga ttggcttagt attgtgaatt gttttttata ggactgactt 5160
tagtatggga agcaatgatg gtaatgagta atttaaagga gaggatgtgt taagaaaagg 5220
taaacatcga gtgggagaaa gggatgttta aagatttcta tttaaagtag aggttcaatt 5280
tatccttgga ataaagatca gaatgcaaaa gataactaaa aatgagatta taagaaagga 5340
ccacacacat aaatggtcat ttgacttcct aaaatcttgg agattttgaa ataggttctc 5400
tggaaagcgt tagtgttctc ttaaaggata attagagtca gttctataaa aacactatct 5460
gtcgccatgt gaactgtagt tataattaat tttagtgtga aagctatcaa tagcagtagt 5520
gacactcact gtatcactgt cggtcacttt ctcatcatcc tgattggtta ctggttcgat 5580
attatctttt attactttat cattattatc tgtaccctta gtacgaattt gctttacgtt 5640
taacgagatc gaaattttag cgcatttggt actctgattg gttggtttat tgtagccggc 5700
caatcacggc gctgaacgcg gtaaagcaaa cttatactaa ggtctcagta acccccaaaa 5760
tcacttcgat gtcgtatttg tttgtgactc gatatcggat cggaaaatct gaagacgctc 5820
ctattgaatg cggtttttat tcctacatgg aacaattaat ttggaatgtt ttcttaaatt 5880
acggtacggt acctatgttt tctcgtacaa aattcaacgc gttcggtgct ctgattggcc 5940
tggttaattc gcaacggcca atcagagcgc cgaacgcggt ctcatttcgt taatgagcac 6000
aatttttata gctgttagca ctcagatttt ag 6032
<210> 200
<211> 3624
<212> DNA
<213> 黄瓜十一星叶甲
<400> 200
aagaataatc atacaacaac tattaccggc aaagtttttt cgaataagtt gttcatggtt 60
taatttgttc caggagacca ctttaattta gagcccactt aaaatgccac ctaaaaacaa 120
atctagaaat gcattttact actttatgga agatttcaaa caaagacaag gatttacttc 180
taaatccatg aaggatgtag cagaagctgc aggtccccat tgggctagga tgtctaaagc 240
agaaagaaga ccttatgaag acagagcaaa caaagagaag ggaggtttga gatatacttg 300
ggatggacaa tgcgttgaag aaatagagaa gagacaaaaa gctgaagaaa ataatattgc 360
taaaatgaaa gatgacatcg actgcacctt gaaatttgcg aaagaaaatt gtcgcctccg 420
agatgaaatg ttcttcttaa tccatatcaa tactttctgt tatcatgcca gtggtaatcg 480
gtattatccc gctgaaattg ctatttcctg ctttagtttg gaagatggcg ttttacctca 540
aaatgttttt cataaaacta ttaagccagg tagactaccg ctggggtatg ctagtgatgc 600
aaaaaagcaa tctgaagatt cacatcaact tcctatccct ttaactggtg aatatgacga 660
caatgtggaa gaagtttata cagaaatgaa agagttttta tcctctaaaa caggaaattc 720
aaaaaatttt cctcccttat atgctaagaa agatatcgtt aaaatggttc aacacgtgtt 780
agatagttgg tgcgctgatt ttgatgagcc gccattattt aaagtttatt atttacagta 840
tatgtttcaa gtactaaaaa actcggtagc taatgacaat gtatggccct tgtattcaat 900
ggcagagacg gaagttgata aggatcttta ttcccatact gctggaatat gttgtgactt 960
tcattctgtt agtgaagctt gtcaattctg tagccgttct gtggttgtca gacttgctta 1020
tacaatttgt gataattgct gttcatttct taatattcct atagagtctg gaagacatgt 1080
acctgatcaa gcttttacag catccagttt gagttcaaga gcttcatcaa aagctagttt 1140
gtattcagct tctagtaaat cttctaagaa gcaatcagtt cgtgaagatg acaaagacac 1200
cgttatatcg ttttctagtg cctctcaatg ggagaacgaa tctgtaatgt cagactcctc 1260
tacatctact ttcaataatg gctttccagg tttgaaatct aaatggacat taagcaattc 1320
tcaacctgta gcatccgact caatgagcca aatgtcaggc ccgagctatg caagtgctat 1380
gggaaataga agacctaata gagatttttc tagtgctgtg ggaaacagag gacctcaaaa 1440
agagtttcct aaaaattccg ataatgatca ctccagtgat tcgactaccg attatttaaa 1500
cagtcaatca tttccggctc tgggaagagg aagaggtttg ggcagaaatc gacgaaattg 1560
atcatttttg actgcaatgg gtcttgttcc acttcataag attgttaaaa gaatatgtaa 1620
ctgtttacat taggcggcta gccaatactc acattgtctc taaaaccact aactgaacaa 1680
ataatcacta actattgctt ttattatatt acagcgaaat taaacaactt tatgagactg 1740
ttttacctta attcacaatt tttctttata tgtatttgaa taaaatagaa acaaatctaa 1800
tacttcctct gtcacttttc tttaaaaggc actagatggc gcctgatatg ttgatataat 1860
gatctaaaat cgcccgccat gtaaaaaaaa caatttgcaa ctttctgctt taactctaga 1920
atactaacct tatttttact atcatttaca ctaaccatcg taaaatttac tacgctggga 1980
aaaaaatgtg gttgctgaaa aagtatttta tataacgcaa ttttacaaat tattttgaac 2040
aaagagatgt ttctaaaacc tgaaaatcac aaaaaaatat aaattctaaa ttttagaact 2100
acactgctga caacaaaggg cgcgatgttc accacaaaat ggacgtcggc agtgttcgca 2160
atgatgagtt gtcattcgac gttttttaga cggacaaaat tgacacatgg cacgttttga 2220
cacagtaggg catattcgtt cttgctgcgg ctccagattt aaaatatttt caataagtaa 2280
ctttaccgag cggttcaaag ttggccaatt tacacgatgc tgtagccaag gtttagtcag 2340
ttgttcagac aaaatggcca taaatttttt tctggtgatg gtttttttct gctgtcgttg 2400
ttggttatga ttataaatta tccaagagtg tatgcaagct atatttaaca tgccatagaa 2460
caggcaaaga ggccatcttg tttttttaga gatgttcatg tttccgcaca tttggtcaaa 2520
ttttggtcaa tccgtacttg gttgcattat aaaatataat gatatctggc tttcctgttc 2580
gatcacattc tactggacca ttgtggattg tcgacagaag aagcacagac ttatttactt 2640
ttgtcttata ggataccatt gttttttcac catcaaagca aaataaggaa tgaccaattt 2700
cacgattttt gagatttatc atctcgggag gtatttctct tttattttgc cgtagggtac 2760
caacaatagt taaatcgtaa ggaggtttta agagctccgt tgctagtggt actgacgtaa 2820
accaattatc catggttacg ttacggttac ttccatactc accctgagtt aattgtttta 2880
cataatattc ttctagaggc tgtccgttga tgttagttcc atcgaacata tatttggtat 2940
ttacgtcaca cagcatctat tttgagtcca tatttggctg gcttgtttgg gatatataga 3000
aaggacatct ttccctaaaa gccaataact actcatcgat tgttaggtat gatccgggct 3060
tataggacgt tttgcagttc tgtataagat catcccatat ttcacgaatg gctgctgata 3120
ggtatgtttc gtttctgcct ggtctagaca ttttatcatc aaaacgtaga cattccataa 3180
gaaattcaaa tctttcccgg tccataattg ctttttatat cgccccagaa attttgtgat 3240
aaagcatttg tcttgtcgct aaatgattgt ccttttgggc agcagcaaaa ataagtagtc 3300
ctagaagtgc atacaattcg tcaatattta gtttcgagtt tgtaagtttt tcgactttat 3360
atttttgtgc cctaacagaa atttcggcat ttgtatattc tagaattttt tccagtgaat 3420
ttggactcat aaaacaatga aaagcatcta agggagtttg tgcttccttg gttttattag 3480
tgaggccttg aacaaaatgt acgaaattac gttttggagt tttagttgcg gcctttggtt 3540
gagaggacca tctgtgtcca tttttacctt ttagactcga cagcaaccga gtttgcttcc 3600
ctttagctgc aatttctgga gatt 3624
<210> 201
<211> 1520
<212> DNA
<213> 马铃薯甲虫
<400> 201
atcactttca ataaattcat tcaattaaag gatttttctt atttgtgaaa ctcttcaact 60
acaattacaa tgcctcctaa gaaaaaacag acttctggaa atgctttctt ccacttcatg 120
caagacttca aaaatagaca aggaagacat ttcaaaaata tgcaagaggt agccgaggct 180
gccagtccgc attggaacag gatgtcaaag gaacaacgaa aaccatacga agagatggct 240
ttaatggcta agaataattt gcgtggaggt aaatttacct ccgatggttt ggatattgaa 300
gtattagaaa atattgagaa ggaagagcag gcaaaaattc aacagatgaa aaatgatatt 360
aattatatat ggaaagtggc gagttcaaat ggaaggattg cagatgaact tatttttatt 420
atacatatca atatatttgt ccattgtacc tctgaagacc gacattatcc agcagaaata 480
gctattgcct gtttcaactt agaggatggt gttttgccgc aaaatgtttt tcatagaata 540
ataaaacctg gaatgctgcc tttaggctat gccagtgacg caaatgcaca ttctaagaaa 600
acccatcagt tacgaccacc gatggctggt gaggacgata ataatattaa agaagttttt 660
gaagaaatga agcactttct ttctaataaa ttatctggat caagaagaat gccaattctt 720
tacaccactg aaaggaatat gaaaatgatt caacatgttt tagatacgtg gtgttacgat 780
tatgacgagg atactcccct ttttaaagtt tatagtttgg aatatatgtt cagaattttg 840
aggaacactg ttgctgggga tgacgtttgg acaaccgata cattcagtac gagagaaata 900
gaaaaagatg tgtacagttt tgctagaggt atttgctgtg agtatcacga agttaccaat 960
gtacccctat attgcagtcg ttcaatttgt gttaggcaag ctttcaatat ttgcgataat 1020
tgctgctccg atctgcgtat caccttatta cctggagtac acttacctga tgctgctctg 1080
acgacggtcc gaagaggatc tagtagaact gcttcatccg cgccttccat ttcttcccaa 1140
tcttcaaaat atactaattt gaagcaatcc acacgtgacg acgattccga aagtgttata 1200
tctttttcga gcaaatcaga atgggataat caatccctaa tctcagaaac gtcaacttcc 1260
gcaactttgg atagcgaagc caattttcct tcacttggag caagaaagaa agtgacctca 1320
tcaccattca acaacttctc ttctaatgac ttgaacagca gtcagagtaa cacctctagc 1380
gtcaaaagtt acgcaggtgc catgggtaca tcagggttga gcaagcgtgc aatggatatg 1440
ccatcatctt ttaccaacac ggaaagaaat tttgaatctt caaaatcttc agattatgct 1500
ccgggccgag gaagaggcgg 1520
<210> 202
<211> 1554
<212> DNA
<213> 黄曲条跳甲
<400> 202
aatatgtgca ttcagcatta acattcaatg tttaaaagtt tcgttattaa attttgaatt 60
taaggaaatc gcaaaccgct tgatttgtta aaatggcgcc caaaagaaaa gaagccccga 120
aaaatgcatt tttctacttc atgctcgatt tccagaaaac acagggccgc aaatttaaaa 180
atttaaaaga ggtttctgaa gcagcaggcc ctcattggtc taaaatgtcg ccggagcaaa 240
gacaacctta cgaagatcgt gcaagaaaag aaagatccgg cgtcagatac accacgactg 300
gcgaaaatat agatgacata gagagaagag aacgtgaaga acaaatgaga atccaagaga 360
tgcaggaaaa aatcaattgc gatttaaaaa tggccaacga ttcgggccag ctccaagaca 420
agctcttctt tatcatacat gttaatattt tctgcgaggc tcaaggtcgg tactatcccg 480
ccgaaatagc tatagcctgc tttagtcttg aggatggagt ccatccacaa aacgttttcc 540
atcatatcat caaacccgga cctcttccct tgggatatac aggaactgct aagctgatat 600
ccgatgaaac acatcatata ccatatcctc tagtcgacga ctgtgacgac accacagctg 660
aggtgtttca tgacatgaaa caatttttat tctcgaaatt accgggtaaa acagttcccg 720
ttttatacgc tgacgagaag ctagcgaaaa tggctcaaaa aatattagac acctggtgcg 780
aagatttcga agacgactgc cgtttcaaag tctttaatct ccaataccta ttcatggagc 840
tgaaaaactt cgctgccaat gacacggttt ggcctaacgg aacgatgagc gccagagaac 900
tcgagaaaga tccatacgta tacgccagag gcatcgtctg cgattttcac gaaaggaata 960
acgtttatac ttattgtagc cgttccatag tgatcagaag cgcgttcgtg atttgtaaca 1020
actgctgtaa gcacttaaac atcgttttaa atcaaggaac tcacgtaccc gatggagcca 1080
tgacgcctgc ttcatctaga gcctccagta gggccagtac ggtttcttcg agatcctcca 1140
aattccagcc ttcgattcat gatgatgatt cggaaactct ggtttcgttc tccgtcaaat 1200
ccgaatggga cagtcgatcg gagatgtcag aaacgtccga attcagcctg actaatgaca 1260
atttccctgg gttggggttc aatccgaaga gcgcgtcttc taattcccaa ccttattatt 1320
caatgtcgca ggaatccaat atgagccagt cttcttctag aagttacgca ggggcaactg 1380
gttcatcgaa atggggcagg gaacctgcaa atagttctgt tccacagaaa ttttccaatt 1440
taaacataca ccaatcttct agtaatttta acgaaggtga caatccagtc ggagctctca 1500
gggatccgag acgttatcct gctttgggaa gaggaagaag atcataagtt tttt 1554
<210> 203
<211> 1574
<212> DNA
<213> 萝卜菜跳甲
<400> 203
gacaacccta cgaagacctt gcaaaaagag accgagccgg tatcagatac accacaacgg 60
gcgaaaatat cgaagacgta gaaagaaggg aacggcaaga acaattggac attcaaaaca 120
tgcaagaaca aatcaattgt gatctaaaag cggccaacga ttcgggccag cttcaagaca 180
agattttctt tattatacac gttaacatat tctgctatca taagactcaa gatcggtatt 240
atcctgccga aatagctata acttgcttta gccttgagga tggagtccat ccgcaaaacg 300
ttttccatca tctcatccgg ccaggacctc ttcccttggg ctacgctgga gaagctagga 360
tgatatccaa cgagacacat cgcatacaga tgcctttggt agacgattgc gatgacaaca 420
cggcagaagt gtttcatgac atgaaacaat ttttaatatc aaaatcacca ggtcccaaat 480
gtattcctgt tttatacgct gacgagaagc taatgaaaat ggcgcaacgt atcttagata 540
cctggtgcga ggatttccag gatgattgtc gtttcaaagt gtacaatctt caatatttat 600
tcatggagct gaaaaatttc gctgctaacg acacagtttg gcccaacgga acaatgagcg 660
ctagagaact cgagaaagat ccatttgtat acgccagagg cattgtatgt gaatttcacg 720
atataaatag cgtttatgtt ttttgtagcc gatccatagt catcagaagc gcgtacgtca 780
tatgcaacaa ctgctgcaag cacttaaaca tcgttttgaa tcctgggact catgtgcccg 840
atggagccct gacgcctgct tcgtccagag cgtctagcag ggccagtacg atttcgtcga 900
gatctactaa attcgagccc tcgactcgcg atgacgattc cgatactctg gtttcgttct 960
ccaccaaatc cgaatgggac agtcgatcgg aaatatcaga aacgtccgaa ttcagcctga 1020
ccaatgacaa tttccctgga ttgggtttca atcccaagag cgcgtcttct aattctcagc 1080
cgtattattc gcaagattct aacacgagcc agtcttcttc cagaagttac gcaggcgcta 1140
ccggcgtacc gaaatggggc aacagatctg taaataatgc tccgtctgct tcacacaata 1200
tgtaccaatc ctctagtaac ttcgatgaag gccacaatag aatcggagct ctgaatgatc 1260
cgagacgtta tcctgcattg ggaagaggaa gacgaacata aaagttttta cccttttttt 1320
aacgcagtaa attttgttaa atttacaatg tatctttaat catttgttca caatgttttt 1380
tttttgcaag agttgatttt atttttgtca cattgttatt aaaatgtgtt tagaagttaa 1440
gtttgagtac tgttacattg ttgttgtcag aacaaatgta aatacagttg taataataat 1500
ttaacttggt acacgaacaa gtgtatgaaa attaatatga ttggtaaagt tgtgtaaaaa 1560
aatgttatta tttt 1574
<210> 204
<211> 1695
<212> DNA
<213> 墨西哥豆瓢虫
<400> 204
gtcagttgct tctacgggta attctacgat tgtgtgtatc agtctgtcta ctttttgtat 60
taaaggaaat aagttttcgg gcatttggtt attgattaag tttccaccag aatgtcgaaa 120
cctaatgcat tctacttctt catgttagat ttcaaatcaa aacatggcag aacttatact 180
aatatgaaag atgttgctgc agcagctgga ccacactgga aaaacttatc aaaagataaa 240
aaaaaagtat atgaagaaag agcaaaagca gccagaggaa ctgaaaaact caattcagat 300
agaagacctg tggaagagat tgagcacatg gaacgtgaag aaatgcggta taaacaatct 360
atgaaagatg acattacgac aacattagca actttgaaga gggaaaatgc actaaagacc 420
catctattct tcatagtaca tatcaatagc ttctgccagc atgatcacac taaaatatac 480
agcccttgtg aaattgctat tgcggcgttt tcattgcaag atggagtgca gcattctaat 540
gtctttcatt cgatgatcaa accaggtctc gtgccccttg gatatgctgc tgttgcacaa 600
cagcattcaa atgaaactca tcaaatcgat ttgccgactg acgatgaaga caaccaggat 660
aatatattcc ttcaagttat tgaattttta tcacgtagaa aaggacattc tgaaaaactt 720
cctattctat atgctgctga aaaaaatatt cctatggtaa gtggtgtatt tgaggattgg 780
tgcactaaag ttgatataga tccaagtcgg tttcgaattt ataatctgca gtatatgttc 840
aaaatattgc gcaacacagt ggcaggaagg gactgttgga catcagatac catgagttat 900
catgaaattg agaaggatgt ctacaactat gcttcaaata tagcttgcga atttcacgaa 960
acctgtgata tactcacttt ctgctctcgt tccattgcta ttcgttatgg ctatacaata 1020
tgtgataatt gttgcgaaga tgcgaatatt gaacttattc cggggcgtca cgtgccagaa 1080
ggctccatga caactccgga tttgagttac gagattgtga aatcatcccg aaaaaagaaa 1140
tctaccaagt atacatctga tagcagtagt attagggacg atagcagtga tgttgacact 1200
ctaatatcaa atgctagtgc aaggtgtcga aacattgata gaagaggtga tccaggtcaa 1260
acaagtagtt actcaactga aagttctgca ggccttgcaa ctgctcaagg ggccagtagt 1320
agtgttcatg ctactggaag ttacgccaga gcaacaacct caaacattgg aagttctgga 1380
ttcagcaaaa ggcctgcagc tatgcccttc aatgtaacag aagatttgaa tgctctgaac 1440
cttaatccag aatcaatgga tgctcatttt cccactattg gcagtagcat gggaagaggt 1500
cgtaatacta accgaaatac tattctaaag ggaagaggaa gaggaaaccc gttccagagg 1560
actacatcga gatcataaac tcgtaataaa ttgcccattt gtaatttttt ctttatgttt 1620
attttaaatt cttatgacaa gttcttattg atgaattaaa gaagtttact attgtttata 1680
ctacttaagg acaat 1695
<210> 205
<211> 1314
<212> DNA
<213> 赤拟谷盗
<400> 205
atgcctccta agaagaataa gaaatccggc aaaaacggct attgggagtt tgttttggat 60
tgtcggaata aacatccaaa taagcaaaat atgcacgaag tacaagaata cgctgcaagg 120
aaatgggcgt ccatgtcaaa ggaagaacgc cgcccgtatg aagaacgtgc cctactagcc 180
cgagaaatgt actctcctgc caggtacaca actgatggta ttgacattga agtggttgag 240
agaaaagagc gagatgaagc aaggaagaaa caagaaatga aagatgacat tacgcgaaca 300
ctgaaagcgg cttatttcgc cactgatctt gacgaaaaaa tattcttagt tattcatatc 360
aatcatctgg cgtattaccc tactgaggat aaatatttta tttgtgaaat agccatagct 420
gctgtaagtc ttaaaaacgg agtggaggat gtgtttcata gaattgtcaa acccggaaaa 480
cttccgttgg ggtattatgg aggagcatta actcattcga aggaaactca ccaaatgctt 540
gagttggttc aggatgaacc ttatgagaac aatactcgtg aagtttttaa tgaaatgact 600
tctttcctga aactttggag aggcaaggga agtgattcta tcgtttatgc ggatgagaaa 660
actcatgaaa tgataacaaa agtcattgat aatttttgcc aagaattcaa ttatcctgac 720
gaaattaaag tctacaactt ccaatatctg ttttttgctt tgagaaactc agtagctgcg 780
cgaaccgtct ggcctacaga aacctacagt tctaccgaac ttgaaaaaga cctctacagc 840
tacacacctg atatttcttg cgaatttcat gaaatgtcgg acatttccgt ctactgtagc 900
aagtcaatcg tgaccagata ctgctacaca ctttgtgacc attgctgtac tgacttaaac 960
attcaactag tggctgggtt tcacgtcccg aaaaattcca gaattgcggt agattcgagc 1020
aggactaatt ccaaagcccc ctcagtctgc agcactgatg attatcgtca agccgcgaga 1080
agccgagttg gttcattcag ttcaacatac gatgagaatt atccagcttt ggggagcagc 1140
agatcaagcg cttcttcggt tgtaagtttc tcctcaatga ggactccccg aaatgtggga 1200
caaccgacca aagctgccac cagaattgaa caatccttca aagccatgtc tgtgagagat 1260
caccgcaatc tgaatttccc cccgaaaggc tacgaaaaac aaaatgaaaa ataa 1314
<210> 206
<211> 1644
<212> DNA
<213> 十二斑褐菌瓢虫
<400> 206
aattgtcatt tccataatct ctgacccaaa aacttaataa aatgccaaag aagaaaataa 60
atcctttttg ggcattcctt atggactata aacgggacaa taaaattgag tcaatcgatg 120
aagcaaaggc taaggctgat gttatatggc gaagcatgac tgaagctgaa aagaagatgt 180
atgctggaag atcaaaacag tctcaagcat ccaatgaggc tccattgact ccgattgaaa 240
ttagagacaa agaagaaata ttattcaagc aaaaaatgct ggaagacata actgccgaat 300
tgaatattgc tcggaaatac aacaaattgt ctagctctaa atttttcatc atacacataa 360
atagtttttg ttctgaccat caaaggaatc gaacatatcc ctgtgaaata ggtatcagca 420
gtttttcact tcagagcggt gtttctaaag atgatgtgta ccattcattt atctatccag 480
gttctttgcc gcctggatat tctggtcaag ctaaaattat ttccgaacaa actcatcaac 540
tgccatggga aaacagtaat ttagataatc agcaagaagt atttgaggaa gttatagact 600
ttctgtcaca aagatcacaa ggttcacaaa aactccctaa actgtatgcc ataagagaaa 660
aagaattgga aattgttaaa aacgttcttg acacatggtg tgatgacttt gatccaaaga 720
ttagtttcac agtgtataat gctcaaacat tgttcacttt gttacataaa aatattattg 780
aaaactcacc atgttcttcg gaagctctgt gttatcgtga atttgaaaaa gatgttcacc 840
agtacgcacc aaaaattgct tgcgagtacc atgaagaatc ggaatgtcct gtttattgtt 900
cgagatcaat agcaataaga tatggttaca ttatttgcca aacttgttct cctagtttgg 960
gtattcaact tgtatctgga tttcacattc caagtaatat gaaacaagct gtaatagaat 1020
caaaaaaaac cttcaagtac cgtgaggttg acagctgtag tacagaaaga gatactgaag 1080
atacttgtac tgttagagac gatagtagtg atgtggatac tttggtctct atagggagga 1140
acagtacata tattgaggat tcagcatcgt ccatttattt tccagaatat gatgatatgg 1200
cacaaaatgt ttctagtgac attcgaacag gaggaagatc gggttttagc cgaaaacctg 1260
ctgctttacc atctactgtg gatagagtat tttctaatct gacattgagc caagattcac 1320
tcggacgaag gaacaatgaa tggccaagct tacgtggaag agggggagga gcaatcccaa 1380
gaaccaaagg aagaggtcaa ccgtttaaga ataagtgata cattgaagct ttacactctt 1440
ttctgttcct aaatttctat atttgttggg ctaatcatta ttgtgttatt tcagttttat 1500
ttactgacaa tgcaactgat tgttgttcaa gaaatgacat tatttcattt caattctttt 1560
ttaaattcat taattttttc tgaaaaattg tactagttta tattattctg ttattgtttt 1620
gtttatgttc atgtttaatt ttct 1644
<210> 207
<211> 2052
<212> DNA
<213> 玉米穗虫
<400> 207
gttgaaagtg tcctgtttca gcagttttct ttaatttcac tttgtcagca atttcaactg 60
catttcaact tttcagtctt gctcttgtat tctgtgcatt agcatcagac agaattcaat 120
tttggtgtgg tagcgtgtta tcttacgtga attgtttctg gcacagtaca aaatgccgaa 180
gaaagctccc aaaaacgctt tcttctattt tatgctgaat tataaagacg aacagcagaa 240
gaaaggcatc aactatggat cattggctga tgtctcagtt gcagcgggcg aagtctggaa 300
gactgtaaac ccacaagaga gagcccgttg ggaagccaaa gccaaacaag agaggacaaa 360
acaaaatatg ccagttgcca agtacacgtc taccggggtc ccgctaacgg tcatcgaaca 420
gcagcagaaa gaaatacaag aggcaatcca gaatgaaatc gatgatatcc ggaatatggt 480
caaaataaag gcttttaatc aaagcattca ggatgaagac ttctacgtaa tagatgtgaa 540
ctattactgc aaagcgggaa acacgtatgt gatcggagag agcactgtgt tacgattcaa 600
cctgaggctt ggataccagg atttctatca tgaactcatc aatcctggtc gcatccccgt 660
cggctacgca tcagacgtga aacacggcag cactgagcta ggtctagaca tgccagacga 720
aactgaaaac cagtctaact acatagaaat actagccaac atcattgact acctgaaaca 780
gaaggataag aacttgaaag ttctgccgcc tttgtacact atgcctgaga aagtgctggc 840
tgttcaagat tttattctgc aaatgagcaa taaagcccgt gaggacgagc atatcttccg 900
cgtctacaag ttggacacgc tgttcttcta cctgataaac agcatcaagt ccaacaagaa 960
cgagggtttc cctaaggagt cgctcgctct catacaactc aagaaggacc ccttcaagta 1020
tacaccggga ttgggctgtg agcatcacga agccaacgag aaatcaatgg agtgcacgag 1080
ttcccgcacg cagcgctggg cgtacaccgt gatggacagc tgctgcccag tcgtgggcat 1140
cgacatccag cccggacaac atgtgcctaa ggaatatgat gttgatggca tcctgaagta 1200
cagagacgct aagaaggtgt ggagcggtcc cactgtggct ggatttaatg atgcatcgtc 1260
atcttgtaac tcaacagtga acgattcttt cgaggcccct cccagtctcc ttgaatatgt 1320
gggcaaaacg aagcgactcg aagctcgcgt gcacgcgccg caacgactcc ccgacgtcaa 1380
tttcgccaag cctatgctac ctcccgagct gacggaggaa gccttcccgg cactgtctgt 1440
gagccacgga agaggtcgcg gattggccgg gaggaggaac gtcagaaaat aaagcattca 1500
atgccaatct tatttttgta ctgttatgta acatatctga cattattatt attaatggct 1560
gttcctaata ttctgtctat ccatttattt actcactata gaattttgac atgagcccca 1620
tacaagtaat gtaaacttcc tatctctggt aagatcaaaa caaaagatag ataaaatata 1680
gggaacggcc acagacagac gaactacaca atacgtgatt gaaattatac tttcaaatgt 1740
catcgaatta tgcaccttat tgtttgtgtg ataaagtgca aaattgtttg aaagttaaac 1800
gggttgatta tgttatgatg gaaaactcta ttggtggatt ttaataacca atcatttgtt 1860
gttttacttc ctcgagacag atcattttac actacttgta tggggcctat gtaaacattc 1920
aatctctgta gggactgaat caacagattc gttatagaaa ttgctaataa gagtactcga 1980
ttagtaagga taaatgtagt ttgtttgtct acagttaagt tttgttataa tttcatccag 2040
cggtactcaa at 2052
<210> 208
<211> 1596
<212> DNA
<213> 筛豆龟蝽
<400> 208
gtttcttaca ttattccctt atccgtcaat tgctagactg atcccggaat tcaaaatcat 60
gccaccaaaa aagaagcaaa agggtggaag gaacggcttt tattatttca tgttggatct 120
acagaagcaa gaagcactaa aggggaaccg ttactccctt caagaaattt caggattagc 180
aaacccttta tggtcggata tgtctttaga ggaaaggaaa ccttttactg atttggccct 240
taaaaatgcc tctaaaaact ctggagactt aactaagaag ttcacttctt taggaataag 300
ttatgctgag ttagacgctg aggaaagaga aatagaggat gccagaaaca ttatgattgc 360
aacaattaag aatactattg caaatctcga tttaaattct actctgaaga gtcatatatt 420
ctttgtgtgt catgcaaatt atttctataa atctgaaaag caagtctatt tcccagctga 480
agttgctgtt gctgcatttt cccttgagct aggtgttctt gcaactttac atttttttgt 540
tgatcctggg aaaatccctc tcggttttaa gtatgaatct tcagaatggt cttcaaggac 600
ccatcgcata cctgtcagtg acccatcttg gaaagaggga ataaaagatc cattagaaat 660
gtatcgaaga gttaaagaat tcatcaagaa atttacagtt gggactgaag ttcctccaat 720
ttatactttg tttgaaacct cgaactcaca ctatcgtctt tctgttgtaa aatctgcctt 780
gaatatgatg tgcgaagcgg catatgaaga tacatcctta tttcgagtat attgtctagc 840
acatttattt tttgaaataa gaaataaatg ttctgcagaa atacctagtg tagctattat 900
gaagaatgaa cttgaaaaag acgtgttttc ctatgcaaga gatttaggat gttattatca 960
tgaggaaaag gatctctcaa tacattgctc actctctatt gtaactaggt gggtttttat 1020
tatttgtgac cattgctgta aacatcttgg tattaaactg attctaggaa gacatgtccc 1080
aaaagataca gctttattag atagggcaca ttatgtgaga aagagtgatg gcttatcacc 1140
gaaacaaaat gttgctgaat ccagcagtaa accattgaca ataattgacc atggaagttt 1200
aaaagaacaa agagctgaag agagaagatc tcaagagatg tacttaagag aaagtgaaaa 1260
aatcagacct ccaaagtact gttacagtga taaaattggt gctagattta gtcaaaatag 1320
ttcctcacaa ttgtctcctg aaataggagc aacatccaat gaaaatgaat ggtgtctggc 1380
taaaggaaag tcttctgggc gaggtcgtgg aattttaagt tattcatctc cagaacatcc 1440
tcaatggcca atatctggac gaggacgagc ttttgcaaat tttcataatt aaagatttta 1500
ttttgttcct ataaaaagca aagtggttct aatttttagc gttaatatat ttttgtttta 1560
ttaaggaaag gtgacagttc ctattttaat gcgata 1596
<210> 209
<211> 1574
<212> DNA
<213> 稻绿蝽
<400> 209
acctcgttgt cagacaggca gaagtggccg cgttaccgta aacaagtaat aacaacaatt 60
atagatacca aataatgcca cagaagaaaa aacaaaaagc tgggaaaaac gggttttatt 120
tttttatgct ggaggttcaa aagcaggaag ctctaaaggg ggttcaatat agtttgcctg 180
aaatttctga aattgcaaat cccatgtgga ctaacatgac cccagaagaa cgtaaaccat 240
ataatgacaa ggcatcaacg agtgtttcca aaaatagcga agacaattca aaaaaatata 300
cttctttagg cattagctat gccgatgtag atgctggaaa gagagagttg gacgaagctc 360
gtgatcttat gatagcaact attaaaaaca ctgtcgcaaa tcttgacatc agaacatctc 420
tgaagactca taagttcttt gtgtgccatg ctaactattt ctataagtct gatcatcata 480
tttattatcc tgctgaaatt gctcttgcag ccttttcttt ggagttgggt gttcttggca 540
ccattcattt ctttgtagat cctggaaaaa tacctcttgg tttcaaatat gaagcggcag 600
attggtcaac tagaactcat ggaattccag taaatgatgc tagttggaat gaagggatta 660
aagatcctct ggaaatgttt ggaatggttc agtcgtttat caagaaattt actgagcccc 720
ctgaagtacc tccaatctac acaatgttcg atactttaaa tgctgacagt cgtctttctg 780
ttgtgaaatc tgcattgaat atgttgtgtg atgctgcaaa tgaagattcc tctctatttc 840
gtgtctattg tttatcacat ttattctatg aaattagaaa taaatgttct gcagaaatac 900
caagtgtagc cataatgaaa agtgaattag acaaagatgt tttctcatat gccagggacc 960
taggttgtta ttatcatgaa gaaaaagatc tttcgatgta ttgttcgctt tctatagtaa 1020
caagatgggt gtttacaatt tgtgatcatt gttgcaaaca tctgggtgta aagctcatcc 1080
ttggaagaca tgtgccaaaa gatacagatc tttcagaaag tgctcgattt atagatgata 1140
aacaatcttc taataagtta gatcatggtg cttcttgtag taaacccttg accatcattg 1200
accatggccg tttaaaagaa caaagagctg aacaaaaacg ggcgcaggat atgcatttgc 1260
gtgcaagtga aaaaataagg cttcctaaat ccagttatag caacaaaatt ggtgccagat 1320
tcagtcaaga aggtgccagt agttctcaaa gctctctgga aacaaaagaa gaatccaatg 1380
aaaatgaatg gtgcatcgta aaaggaaaag gatttggacg cggacgtggt ttcactgtag 1440
aaaaatcttc agctgaagag cctatctggc cagtttctgg aaggggtcga gcatttcaac 1500
aataaatttt aaaagacatt ttaatataga caaaaaattt taataatttt gatacaagtt 1560
ttttatactg tttt 1574
<210> 210
<211> 1640
<212> DNA
<213> 褐臭蝽
<400> 210
tagattcacg tttggttgaa acctctaaga ctgacagacc aaaaaggccg cttttgttta 60
acttgtttat atcgttcacc caggactgtc agtatgccac agaaaaaaaa acaaaaagct 120
gggaaaaatg ggttttatta ttttatgtta gaagtacaaa agcaagaagc tcataaaggc 180
aataagtatt ctttgcctga aatttctgaa attgcaaatc cattgtggtc tgctatgtca 240
ccagaagaac gtaagccata taatgacaaa gcactaatga acgtttccaa aagtaatgaa 300
gacttaacaa agaaatatac ttctttggga attagttatg ctgaactaga tgccgaacaa 360
agagagttgg atgaagcccg aaatatgatg attgctacaa ttaaaaatac aatagcaaat 420
cttgacccca gaaattctct gaagactcac acattctttg tatgccattc taattatttt 480
tataagactg atcatcatgt ttattatcct gctgaaatag cttttgctgc cttcaatctg 540
gagcttggtg ttctagccac cctacatttc tttgtagacc ctggaaagat tcctcttggt 600
tataaatatg aagctgcaga ttggtcagct aggactcatg gaattccagt aaatgatcct 660
agttggaatg aaggggtcaa agatcctcta gaaatgttca gaagggttaa ggatttcctt 720
aagaattttg ctggagactc tgaagtacct cctatctaca caatgtttga taatttaaat 780
gctaacagcc gtcattctgt agtaaaatct gctttgaatt tgatgtgtga tgctgcaaat 840
gaagattcct ctctatttcg tgtttattgt ttaccccatt tattctttga gattagaaat 900
aaatgttctg caggaatacc aagtgtggcc ataatgaaaa gtgagttaga taaggatgtt 960
ttttcatatg gcagggatat aggatgttac tatcatgaag aaaaagatct ttctatgaat 1020
tgttcacttt ccatagtaac tagatgggta tttaccattt gtgatcattg ttgcaaacat 1080
cttggtataa agctcatcct agggagacat gttccaaaag atacagacat ttcagaaagt 1140
gctcgattta tagaagataa atattctcca aataagcttg atcatagtga ttcctatagt 1200
aaacctttaa ccatcattga ccatggacgg ttaaaagaac aaagagcaga acaaaaaaga 1260
attcaggata aacacttgcg tgaaagtgaa aaaattaggc ttccaaaatc tgtttacagc 1320
aacaagattg gtgccagatt cagtcaagat tcaggcgcta atagttctca aagctctttg 1380
gaaatgaaag gaacaaaatc agaagctaat gaaactgatt ggtgcattgt taaagggaga 1440
agcttagggc gtggacgtgg ctttatccca gaagaacctt caactgaaga gcctaactgg 1500
ccagtttctg gaagaggaag agccttccaa taataattta tttgagaaat tttaatatag 1560
acaaaaaact ttgtaatttt aatatggacg aaaaagattg ttatttttaa tattttttta 1620
tgtaacagtt tactatactg 1640
<210> 211
<211> 1500
<212> DNA
<213> 狡小花蝽
<400> 211
ttctggcttg caacttgaaa aaaagactcg tgattttact tgtagtctca actctcaaga 60
gtcacgctgg aaaagtaaag ttgagctgct tatttgttgt caacgtacca tttttcgtaa 120
tatgcctaat aaaaagaaga ataaagccgt gagaaacggt tatttttatt ttatgcaaga 180
tgtccgtgaa cgcgaggcac tgaaaggcca ttattttcca agtcaaaagc aagtcgccga 240
tttttgtgct ccactttggg cgaatctctc tccacaagaa aaggaagatt acaacgatca 300
tgccaagcag aaaaggcttg aagagaaaaa ttctgtcgat gaagctgcca ctaagttcaa 360
ttcaatggga atatctattg cccatcaaga cgctgaatta cgtgaacaag aagaaaacga 420
aatgattatg aaaagtcaca ttaaaaactt aatgaccgat tgtggaaaag ctggaattat 480
gacgtacaag ttttatttgt gccatgtcaa ctacgcattt aaagtcggcg ataagaagtt 540
cgctcctgct gaattagctt tggtcgaatt ctcactttat gagggaatca caaatagatt 600
tcacactttt attcatcctg gaaaaattcc tttggggctc agatacgagg cgatcgaatg 660
gtccaatatg tcacaccaaa tacctgttga gggtggattg ggtgaaaaat tggataaaaa 720
tatcttcaac tccatcatct cattcttaaa tagcaaaggt agaaataaag cgttgccggt 780
tatttacacg atgccagatt cgttaaattc aaacgattct atgaaggccg tcgtgagtgc 840
ttttcagcag ttatgcgagg tggctgaagc agaccctgat attttccgca tttatgaatt 900
acctcatttg ttctacgaac tccgcaataa atccgtagaa gacggccaag tggattatag 960
atttccgagc gttgccttag ccagagagct tttacaacgt catgtttata gctcttgtag 1020
caatatggga tgtgaatttc atgttcaaaa tgactatgaa ttgaaatgct cattgagtgc 1080
tgtgacccga tgggcatacg ccatttgcga tcactgctgt caatatttgg gaattaaagt 1140
tatccctggt gtacacatcc ctatccccac cctactgggt aacgaaggat attctgctgc 1200
tgtgccttgt gtatcgccaa aaggcaattt gccaactcat gcttctcttc ctgaacctgc 1260
ggaagttcat gaacaattgt cttctgattg ccctgatgaa tcttttggat ataaatatga 1320
tgaagacgat ttccccggtt taggtggcaa ttcaaagaat gtaagaagtg aaaacgcccg 1380
ttcttggggt aatccatgga gaaaatgatt ctttttatat taaaactttt aataaataat 1440
ttacctttct ttatagacaa agaaatacat ttattttaac ttctgaattg tttaaaaaaa 1500
<210> 212
<211> 1810
<212> DNA
<213> 烟草天蛾
<400> 212
tgctattaat tttagctcct tacgatttac gaattggcat tttcaactaa aagttttaca 60
gctatatatt tgaaatatat atttataatt gaataagatt aataaaggtt gactttagac 120
aattattcta aatttaaact ggtaccaata ggaagatgcc gaagaaaccg ccaagaaatg 180
cattctacta ctacatgctg gacttcaggg aaaaccagaa gaagttggac atcacttacc 240
aaagtctaaa ggaggtggca gaagctgcag gaccatcctg gaagaccatc tcaccaaccg 300
caaaagccaa atacgaagct atggctagaa aagagagggg tacaaaccag aagttcacat 360
ctactggtgt gcctctcgcg tatttggagc agcaacaaaa agaaatgcag cttgtggagg 420
aggctgaact caaagatatc aagaacatta tcaatcttaa gtgttttaat caaagtattc 480
tggatgaaga cttcttcctg ttggatgtga ttgagttgtg caaagctgga ccggactatc 540
tcattggaga gttcactgtc ttacagttta acctgcgtga aggcattaag aacagttatc 600
acgagtttgt tagaccagtt agcgtgacta tactgaccta cggccttgac acgctggacg 660
acagcctacc caaggagaac tacgtgcaga tcctggccaa catcatagac tatctgaaac 720
agaatgatcg tggcacgaac acactaccgc ccatattcac aatgccagag aaagttgagc 780
cgatacagaa ttttatatgg cagctgtgta acagagctgc cgaggacgac gcgttattcc 840
gcgtatacaa gctggacacg atgttcttca cgctggccaa cgcgattcgc agcggcgaca 900
acgaggggtt cccgaaggag tcgctcgcca ccgcgcagct gaacaaggac ttgttcaagt 960
acacgcccgg gttggcttgc gagcaccatg agaccgtgga caaaagcaac gagtgcacat 1020
cctctcgcgt gaagcgctgg gcatacacaa tcctggacgt gtgctgccca ctgctgggca 1080
tcgccgcgca gcccgggaaa cacgtgccgc acgacttcga tatagacagc atcctctcgt 1140
acaaggaaga gaagcgtgaa cgagcgctac cgagtgtagc tcgtcggcag gtgccgcacg 1200
acagtatgtc gtcttgcagt tcgtcgatca tcaacgacac agtcgacgat tccaccgtgt 1260
atagctttaa cgcctcccac accagtgctc caaagggcaa gaggacctac acgccgctgc 1320
gtatgccgag gactgattat tcgcaacgtc tccaacaagc ccccgagctg accgagacga 1380
acttcccaac gctcggcagt acaggacacg gcagaggtcg cggcctcgcg agcagtttcg 1440
atcggatgaa cttgaagaag taaataaaaa ttaagaataa cttgcaatgt gccaacgtat 1500
caacaagatt tcattgtgaa tttgtaataa gctaaaataa atgactattt ttctgtgttt 1560
tatgaatctc ggtgtttaag tatacatggt gctgtaaaag cgtaaatatt gaacttttca 1620
gggcacgctg tataacgtag tagttgtgac attgtttata cgcacaaaaa gaactatcaa 1680
aaaacttagt aaatatgtct taagtcatat ctaaatttgt tccatcatca ttgaacatat 1740
tttgtaccag agtgaataga ggcacagtca taaactcgcc atgttgataa cttcatacaa 1800
ttggaaacgt 1810
<210> 213
<211> 4514
<212> DNA
<213> 草地贪夜蛾
<400> 213
cgggaattgc acgaattgaa ccggctacaa aaaaagacgt tttgcgaaat agaaattatt 60
ttgcgttgtg ccttgactgc ttagactgca ctaagtgtct gcatctgcga acgaaaccta 120
cccattgtac tttgtaatta atcacctgct atattatttt tttaattcaa ttcgactaat 180
atgcctaaga aaaagaccgc ctactggttc ttcttacaag atttcaaaga agaacaaagg 240
ctaaaaggca ttacgtatac ggaaaaacag gaattggata aagatgccga taaagcatgg 300
aggagtctac ctccttcagt tcgtgctaag tacgaacaaa cggcgaagaa tgagaaggaa 360
aaaaacaatg taactgttat aaagtatacg tctaatggca tcccgttgac tgtgattgaa 420
aaacaaaaac aagaacacga gatggcgcaa atcaatgagg ttcaagatat tacaaatata 480
attaaaacga agtccttcaa ccaagaaatt ttagatgaat acttctacat catcgacgta 540
aattcttatt gtaagtggga tgacaaatat ttgattggcg agtgtaccgt tttgaggttc 600
aatctgcgga atggtattcg agattattac ccagtattga tcaaccctgg agaaataccg 660
atggggtacg cgtcggatgt aagagtggcg tgtatagagt tagggctgga catgccggat 720
gcagaatcac cgagtaacac gatggacatc ctcgccaata tcatcgactt ccttagacag 780
aaggacctga aggccggatc tctaccgcct ctgttcacta tgtcggagaa agtactgcct 840
actaagagct tcctggaaca gatgtgctac acagctcatg aggatgaaat gatcttccgt 900
gtgtatcgcc tggagaagtt gttgttccat ctggtgaata gcctgaagtc cgacaagtac 960
gacgggttac ctcatgagtc tctcgcgctc gagatactga agaaagactg tttcagatac 1020
agcgaaggac tggcttgcag acaccacgca gccagagaca aggccacgga gtgcacggac 1080
tcgcgcgtgt tccgctgggc gtacagcatc atggacgagt gctgcgcccc cgcaggcatc 1140
actccccgct ccgggaccca cgtgccaaag gacatgaaca tcgaaaaata caagcttcta 1200
aggaacccta ctcctagagc tacagtcgct ggatatgagg cggcaccatc gtcctccaac 1260
accacggcca acgattctat gtgggaaggg agctttgaag cctctctcgt cagtgagccg 1320
tcgggagctt ggggtgaacc ttctggagct tggggtgaac cttctggaag tcaggcggcg 1380
agtgcctggc ctcaacccac tggcattccg tcgtcggaaa cctggccaca actccctggc 1440
agtgggccgt cgagtgcctg gcctctacct tctggtagcc agtcgtcgaa tgaatggccc 1500
gggctccctc ggagtcagtc gtcgagagcc cgcggtcaac actccggcag cggtcgtggt 1560
ggccgggaat ctgagtctgg gaggcgaggg cgccgcacac acgagcctat gcgcatgccg 1620
caagccgact attcccagaa cctccaggcg acgagcgaat taatggagaa acacagtccg 1680
ctcctaccag gccttggacg tggtcgcaat ctagctggca gcttgagcaa gatgaacatc 1740
aataagaaaa agtaaagcat tgaatgccat ttaaatttct accctattat tattatgtaa 1800
tgaatctgat ttactttgta agtatagtgt tgtcagatgg gctgtgttta caccgactta 1860
ggtggacaac acaaatatca tacaggaata tcgggaaaaa atacttggaa ccagtggcgg 1920
aataaattga caacccggca aagttctcag taactcaatt cttcttttta tattaggaga 1980
ttgggctcat ccgatgaatt tgccacgggt ctcgaatcat acagttactg cttaaaattc 2040
tatttacatt ctacgactat agagctatgg acaaaaagtc tattgattta tctctttttt 2100
aatctataat ccataaacaa tattgacaac tatcctacct cacaactatt tgaccaaatt 2160
gctttaatat cttaaatcta ttgacaaata actagtaatg tataaaaata ttttcctcta 2220
tcatattttc aaaagagtgt agtttaaatg aaactatatt tttagacatc tatcccctaa 2280
tcttataaat taagccatgt aagtctagga ccattacatt acattgacca actttttcca 2340
tagctccaat tattaaccat acgtatctat agcacaatat agcttaacga tagctaagcc 2400
catctggcta cacgaaaaca tgattatttt gtattacaat ccgacgatac tcaatctttt 2460
cagtctaact gctcaatgta tatgtaaata atgtggcaaa caataagtca tttatatatg 2520
taattgactg attaggctac ataggattgc ctaaatatat ttaagatata tagattgtat 2580
tggtatgtag gtacttaata aggaaaactg aggaacctta agactagtga agtgtgatat 2640
taggatggtc taggagtgta ctaagtacgt tttgaaaagt caaaattttg agggaaggct 2700
ggtttattaa cgtattgaca ttcgatgtca ataaattcat tttatttgga tttaatcctt 2760
taaacttacc tacaatttga tacgggagta tcaaggtagg attaaaattt aaaataaaat 2820
ttgaataata ttcaaaatta aatggtttac ttcattaatt acccattaaa gttaactctt 2880
cgagtcgttt gcattacggg tatttcgttg ttccttttcc gtaaaacatt gagtatcgtt 2940
ggatttactt attaattaaa aactttttga tatttttata atgtgggatt tatgctaatc 3000
tccgtacatt ccgtattcat attgactgtt ggaaagccaa tttgtaaacc cggaattatc 3060
gtagatccca catgtttaga atagttctga caatcggaac ttaaaaattc tgaaagaaac 3120
cttttacacg tgttatattt tacactatcg tctatcgttt tagtaaaact tcgtgcaggg 3180
ttgacaaaga gttagttata agtataattt tgttgctttt cattgaaaac atattttatt 3240
taccctaagt tttatataag ttttcaactg ggctaagaat gtgactacat ttacataaaa 3300
ataagaagaa attccaccaa agttctctcg tttgtctggt atagattcag tatcgaatgg 3360
ttaaatgaca ttacacattt attaaagatg tcttgagaaa taatcagcat cttattaaaa 3420
agtataatta aataatgttc aatgttcata tatgtctatt gtagtggttc cagaattccc 3480
cccaccgacc tgtagtacgt agatgcagaa tagacacaaa atatctcaga tgttgtacga 3540
tttagctgac cggtcaatat agctcaccac gccaaatttt tagtgccacc tgtgctcaaa 3600
aacttaacct aatcagttgg ttatatcacg tattccttca gtacgagcat tactttgtga 3660
tcgtcttaaa tagtggtatc tttgtctatc tagtcggaat gaggcagttt tctcattata 3720
ttcagtagta tatagtttgg acaaaatctc aatgttggta gtgtttagta tacgtaagaa 3780
agtctatata aatcgcggtg atttaaaaaa aaaaaataac caaatgatgc tttggtccag 3840
aacttgcctg agttccaagg cttcgaacat ataacaacat gttcagtaaa atttaaattc 3900
tttctgtgtt ggattgccgc gagtccagtg ttacattcat agggatatac ccttttaata 3960
ctataagtac tatcatagtg taggtatcaa taaatatgta ttttgatatc gtatttatta 4020
atctccttta ctcgttttga aagcggatat tatagaaaag ggggtttata aacccaattt 4080
ggacaagaga tataattgac aatgtatgtt tcccgtcgaa attattttga atagtatttg 4140
tagttatata aggtacataa tacctatata caattttata tagttcttcg catgtggcgt 4200
tggtttattt aagggtaata actatataac ataactacag tcatagatat catatttacc 4260
tatattcaaa tataataaaa tctttcaata aactatgtaa tgttttctgg ctcaataata 4320
tttgaaaatg gcaacctcta agtcaactag cgccacctat atattaaagc ttaatatgtg 4380
tgatatgtat acgtagtagt gtgtttatgg atataaaacc tgtctgactg tagattacaa 4440
atatctatta cgaattctat gtgtgatagc cataggtacc tatgtagtac tttcaatgtg 4500
tgtttaatga ataa 4514
<210> 214
<211> 2917
<212> DNA
<213> 欧洲玉米螟
<400> 214
aaaaatttgg ttggtttgca aaaattcgct tgtgccggca aatttatttt gaccaccgtc 60
tcatattcgt tatattgtaa tattttacat actctctgtg ttattctact ctccgtgacg 120
atacgatagc catttgagta gggcatttat ttcaatatgc cgaaaagaca agtaaggaac 180
gcattttatt tctatatgat tgacttcaga gaacaacaaa agaaaattgg aattcagtat 240
gggaacataa gagaagtctc tgaagctgca ggtccttcgt ggagggaggc accaccaact 300
gtacgcgcca agtttgaaga aatggctcgc caagaaaaat tgaaaagaaa tgtacctgac 360
cagaagttta cttcaacagg gataccattt tctgagatag aacgacaaga gagagagttg 420
cgtgaagcag aggaagccga gagagaatac attaataatg ttgttaaact aaagtctttt 480
aattcagaaa ttatacatga gtacttttat gtgatggatg tgaactacta ctgtaagttc 540
aactcggatt acctaatagg ggaatgtacc gctctacgct tcaatgttaa aaatggcatc 600
aaagatcaat ttcatactat gatcaaccca ggtccaatac cagttggata tgcttatgat 660
gtcaaactcg gagctcaaga acttgggctg gatatgccgg atgcaattac ttataagaca 720
ttgccataca tagacacttt agccatcatc gtcgactacc tgaagcatgg ccttccgact 780
tctagcaaga cagtaccacc gatttacaca atgccagaga gggtgcaacc agtcctgaat 840
tttatattgc aaatgtgtgc aaagacaggt gaagacgacg gcatgttccg cgtgtacaag 900
ctggacacgc tgttctacac gctgctgaat ggcgtcaaga accgcggcga cgagggattc 960
ccccgcgagt cgttggcgct cgcgcagctg aagaaggacc cattccggta cacgcccggc 1020
ttggggtgcc ggcaccacga ggacatagac aaggctgcgg agtgcactac ctctcgcgtg 1080
aagcgctggg cctacaccat catggacagc tgctgccccg tggtcggcgt ggacatgctg 1140
ccgtcgaagc atgtccccgg cgacttcgac ctggacacca tcataaattt tagagagcaa 1200
aagaaagaaa gagctgggcc gtcagtggct gtccctcggt ggggggaacc ttctaacgcc 1260
tcagccagcg atatcaatca gtcttcgttc aacgagtctg ctaatacttc agcctcaact 1320
tcagctcctt cgacttcatc aaagaaggaa aaaaaagccc agcctcctct acgtatgcca 1380
aagacagatt attcgcaaac ccttcgccca gcgcccgagc tcaccgaagc caacttccca 1440
agtttctctg ctggacgcgg ccgtggctta gcgagcagct taaacaaaat gcatattgga 1500
aaaccaaaga agtaaacgta gacagtctac ctttaatctt gtcttgacct gtactcaatc 1560
ttgactgcta actttatttt aagacttcct cttgtgctgt gggacttaga tacaaatatt 1620
ctattaatgt ttacacttcg tgtatctaat acacttaagt caggacttgt taccctttta 1680
ggattgtgtc tacaaaatta ttgggcgagc ttatatggcc gacagggtat ggtcatttaa 1740
tgggtgaaag ttcgatatgg atatttctat aatgcagcta ttcatcacta tcgaatatgt 1800
atttagaagc taaattttag ccatgtatgc tcgctatcgt gcattatggg tgcataaaaa 1860
ataaaattag tctcttgata tgacaccttt acatttcggt cttggttcga cttttacgtg 1920
catttatctg ctatgtatgt ctgatatgga gcaaagcaag tattaataaa atatttcata 1980
gtaaataata gaatattcag tagtaaataa ccgtatataa tatacctaat aatactatat 2040
tcctgtgtat aaagcttgga tacattgtgg tgaaatgacc ctcagtgacg cacaacgctc 2100
gaactacttg acgccacgtt gtcgcactgt gtcaagcatg acatagattt acttaacatt 2160
ttcctttatc cgagatacca cttcatttgt ttcctagtgt gtccaatgtt ttaaattgac 2220
tcaacaaaaa gatttcaaaa attgtcgtag cgaatgattg ttaatgtctc tagtgtatcg 2280
gcgttcaatg acttaagtat attgctatct ctagatgatt gtctatttca ttgatcaaat 2340
atgagaatac atgattatgt tttatgtcga tgtgaggtta taagtaaatg aatagcattt 2400
agtattaaat ttaactaaac atataaaata tgatattatc tctacatgtt atttgatgtt 2460
gtaacaatcg atgtgataaa taatattaag tcaaatactt ttgtgattgc tttagttatt 2520
taagcattag tgccatttcc gcttcctctt ctctgagata taattttcga aagaaacaaa 2580
actattttta tatttcgttt attaaatctt taaaagaagt gtatcatatt tttaaggttg 2640
gccaagtaga ataagtttta atgtaatcac caaagtgata cttgatctta atgttgatat 2700
tatttcggtt ttttttacga taaagactct agcaaacagc cggctagagt gtaatgtgaa 2760
gtatacgtac ttattgatag ttagaaccta ccgctttgtc gcactcgtgt gggctgtgct 2820
agaggcttta gtaactaagt ttcttatgtt aaaatgttga agtatttgtt tgtacttgaa 2880
tttatagttt cgtatgacaa cttaatgacg attaaaa 2917
<210> 215
<211> 1713
<212> DNA
<213> 豆荚盲蝽
<400> 215
accaaagttc aaagcattga atactaacgt cgttctgagc aattcaaagt caaagagtgc 60
atgaaactgc tgcagatgtg tgtcctctgg ggacaccttg ccctgacgtc caaactcccc 120
actacaccaa ggataaggat caaggatcgc taaggataac ggaactccat ttgaacagtt 180
gacttacctc tctgcaatca tgggcagtaa aaacaaagga aggaacggat tttactactt 240
catggtagat ttgcagaaag aaaaggctga cgatgggcac ttctatagaa tggatgagat 300
gtcaggaatt gctggcccac tctggaagaa tttatcgctg gatgaaaggg aatattacaa 360
cagaagggct aaggaagaaa aattaaaagg cgcctcggat atggatcgca agtacaattc 420
tctgggagtt agtttttcgg tcgtcgaagg cattgagaga gagttggacg aacaggagag 480
agtcatgaaa gctaccatca ggaatattgc acagtcctcc agtccggagg cactcatccg 540
aaaacctttt tatttctgcc acgtaaacta ctacttccaa cccgataaag atagctacca 600
acctgcagaa attgctctcg cggaattcac cctggaagac ggccttcgag agacgaggca 660
tttcattctc cacccaggta aaatccctct ggggatgaaa gccgaggcac aaacctgggc 720
tgataaaact cacgggatcc gactagtaga caagcctggc gaggaaatcg aacgtgaagg 780
agacttcgtc aaagtatttt cgaacattgt gaacttcttg aagaacgacg ctggagtggg 840
gcagggagga gggggaatgg tgctcccagt gatgtattcc atgccggatt cgctgaacaa 900
caataccagt ctgtctgctg tgaagtcctg catgaacttc ctaagtggat gtgcccatga 960
gaatcgcgat ctcttcaggg tctacccgct accagagttg ttctatcatt tgtggaagaa 1020
gttcgagaag aagggcgacg aaacacccac ggttgctgtc ttggagaacg aagttgaaaa 1080
agacattttc agtggcgctg ctgatttagg gtgtgagttc cacgaagcga aggacctgag 1140
cctccactgc agtctgtcaa tggtgaagcg ttgggtgttc acagtatgcg atttcgtcaa 1200
caaaatgacg ggtatagaga cgattcctgg atgtcacgcg cctgacaatg cggacctcca 1260
atgggctgcc atacaatcca gcaagcgcat gaacccagac gtgaaagttg gatacgtcga 1320
tccaccaccg ccaaaatctc ctcccaagtt tgttgatttg acgaaaatgc aggaactgag 1380
gatacgagaa aaagaagctg tggaagaaga actctcccaa gccgaacgtc ttccacctcc 1440
tgtctctgct gtcttcagag gagggccgct ccctcctaat ctcaatgata gagatttccc 1500
tcctattgga acgagtggtc gcggaatgta cctccccaga tccaagtggc cagcagtctc 1560
ttcgcaaaga gggaggcgtc agtaatgaaa gtggaagaac ctatttcgaa acctacgcga 1620
ctttggttga ttataaacca atctaccctc gatttattgt tcatttttca attgttttat 1680
tgtttattcg tgccagaatc gtttaggata ttc 1713
<210> 216
<211> 601
<212> DNA
<213> 棉红铃虫
<400> 216
tcaactttca gttcagttcg ttcgttcagt cagttgattg gaaaaaaaag ttagttctaa 60
aatctaaatt atttttgaag gaaaaagttg atcaaaatgc cgaaaaagcc accaagaaat 120
gcattttact attttatgtt agattttaaa gaacaacaaa gaaagaaggg tataaactat 180
gggaatatga acgaagtagc gcaggcggcg ggccccgaat ggacgtcagc aaaaccacaa 240
gtgcgcgcga aattcgaagc gatagcgaag gctgagaagg cgaaatcaaa tgtgccggag 300
cagaagttca catcaacggg gcagtcgctg gccgagctgg aggcgttgga gaacgagcgg 360
cgcgcggccg agaaagccga ggagcgagac atcctcaact ttgtcaagca gaagagtgtc 420
gacggcagta tactggacga ggacatgtat ctcatggatg tgaactacta ctgcaagact 480
ggatcctcat acctgatcgg ggaattggca ctgctgcgct tctctatcag agacggcatc 540
aagaacactt accatgagat cattaaccca ggtggcatcc ccatggggta cgcgctggac 600
g 601
<210> 217
<211> 1396
<212> DNA
<213> 北方玉米根虫
<400> 217
aaaacattca ccacatcaac acgtgaatta atattcgaaa atggagtacg aaaatacaca 60
acaaaatata aacttcgtac cgtgtgtaag atgggttaaa cgaggagtgg ccaattcaag 120
cccagtaaaa ttgcaactgt cgaaaaacga gctggctcaa attattaatg acaccaagat 180
taaattacaa gaatccaatg aaaatgaaga cgagcccatg gaagaaggtg aaacgtctca 240
aacagatgag tttgccttag aggattacga taaagaagac gaaaatgagg acactgcaaa 300
tgctttagga attggatcat tggcagaact cgataatgat gctgcagaca atttttctga 360
gtcagacgat tctgaaaaag aagatgataa aatcaaacca tctgacaatc tcatactagt 420
aggacatgta gaaggggatg caagtctatt ggaagtctac atatacaatg aacaagaaga 480
gtcattgtat gttcatcatg atattatgtt atcatccttt cctctgtgtt tagaaccgct 540
aaactatgaa ccgaagatgc ccaaaggaaa ttattgtgca gtgggatcaa tgtcacctgt 600
tatagaggtc tgggatgtag atattatgaa tgttattgaa ccttcattta ctttagggag 660
acctgctagt aaaaagaaga acaaggaaca tataggccat acggatgcag tgctttcatt 720
agcttggaac aaaacctttg aacatgtgtt ggctagtgga tctgtagatc aaacaataca 780
cttgtgggat atggaaatca aaaaaccaag tacaaccatt aaatccttcc aagaaaaagt 840
tcaatgtctc gaatggcatc ctttagaggc acaaacactt ttaggtggag gttgtgacaa 900
atctgcaaga gtatttgatg gcaggacccc tgaaacccac caaacttggc tacttgatgg 960
agaagctgaa aggctatgct ggaacccttt agaacctttc acatttttgg caggtaccag 1020
cagtgggtct gtacagtgtt tcgactgtag aaaaggacag ctatggtcag ttaaagcaca 1080
cagcaaggaa gtaacagggt tagtccttag caaacaatgc caaggattgt tgattacttc 1140
ttctacagat gaaacagtta aaatttggga cttggctaca ctggaagctg aacctaagct 1200
tgttaatgaa aaggagttta atatggggaa tattcactgt ttggatttat gtccagactt 1260
gccgtttgtt atatctgtag gtggagataa gaagtcaaat aattttactg tttttgatgt 1320
acagaatatt gatgttgtta aacacacatt tgggccaaga ggattggtac agctagttcc 1380
agatacagaa gaaaat 1396
<210> 218
<211> 1402
<212> DNA
<213> 黄瓜十一星叶甲
<400> 218
caaacatgta tataaaaaca tatttcaaca cgtgaaatat tcgagaatgg agtacgaaaa 60
tacacaacaa aatataaact tcgtaccatg tgtacgatgg gttaaacgag gagtggccaa 120
ttcaaatccg gtaaaactgc aattgtcgaa aaacgagctg gctcaaataa ttaatgacac 180
taagattaaa ttacaagaat ccaatgaaaa tgaagatgag ccaatggaag aaggtgaagc 240
gtctcaagca gatgagtttg ccttagagaa ttacgataaa gaggacgaaa atcaggatac 300
tgcaaatgct ttaggaattg gatcattggc agaacttgat aatgatgctg cagacaactt 360
ttctgagtca gacgattctg aaaaagaaga tgataaaatc aaaccatctg acaatctcat 420
actagtggga catgtagaag gagatgcaag tctattggaa gtctacatat acaacgaaca 480
agaagagtca ttgtatgttc atcatgatat tctgttatca tcgtttcctc tgtgcttaga 540
accattaaac tatgaaccaa agatgcccaa aggaaattac tgtgcagtgg gatcaatgtc 600
acctgttata gaggtctggg atgtagatat tatgaatgtt attgaacctt cctttacctt 660
gggaaggcct gctagtaaaa agaagaacaa ggaacatata ggtcatacgg atgcagtact 720
ttcattagct tggaacaaaa cctttgaaca tgtgttagct agtggatctg tagatcaaac 780
aatacacttg tgggatatgg aaatcaaaaa accaagtaca accattaaat ccttccaaga 840
aaaagttcaa tgtctcgaat ggcatcctct agaggcacaa acacttttag gtggaggttg 900
tgacaaatct gcaagagtat ttgatggcag gactcccgaa acccaccaaa cttggctact 960
tgatggagaa gctgaaaggc tttgctggaa tccattagaa cctttcacat ttttagcagg 1020
taccagcagt gggtctgtac aatgttttga ctgtagaaaa ggacagctat ggtctgttaa 1080
agcacacagc aaggaagtta ctgggttagt acttagcaaa caatgtcaag gattgctgat 1140
tacttcttct acagatgaaa cagttaaaat ttgggacttg gctacattag aagctgaacc 1200
taagcttgtt catgaaaagg agtttaatat ggggaatatt cactgtttgg atttatgtcc 1260
agacttgccg tttgctatat ctgtaggtgg agataagaag tcaaataact ttagtgtttt 1320
tgatgtacag aatgttgatg ttgttaaaca cacatttgga ccgcgaggat tggtacagct 1380
agttccagat acagaagaaa at 1402
<210> 219
<211> 1436
<212> DNA
<213> 黄曲条跳甲
<400> 219
aatttcaatc gaaaaaacat tgcacgtgaa gtaaaacaaa tttttcaatt tcacaatgga 60
aatagatgca cagacagaat caaatataaa cttcatacca tgcgtaaaat gggtaagaaa 120
aggtatagct aacacgaatc ccgtgaaact ccaactgtcg aaaaacgagt tagtcgatat 180
aataaatgaa acgaaagtga agctccaaga agctgaagaa aatgacgagg aacttatgga 240
aagcgatgta ccgagacaag atgaattcaa tctggaacaa tacgatgaag atgaagccga 300
agaaaacact gcaaacgcat tagggatagg ctcgttagct gaactagata atgcagccga 360
ggataatttc tccgagtccg acgattctga taaagaagac gaagtcatta aacctacaga 420
taatctgata ttggtggcgc acgtagaagg agacgccagt ttcttggagg tgtacgttta 480
caatgaagaa gatgaatctt tgtatgtaca tcacgacatt cttttaccat catttccgct 540
ttgtctcgaa cacttgaact acgaacccaa aatgcctaag gggaactact gcgcaatagg 600
atccatgtcg cctattatag aaatttggga tttagatatt atcaatgtaa tcgaacctag 660
ttttacgtta ggtcaagtaa agacgaagaa aagcaacaaa gttataggac acacagatgc 720
agttttagca ttggcttgga acaaaacatt cgaccatgta atagcaagtg gatcagtaga 780
ccaaacaatt catctatggg atatggaaat taaaaaaccc agtacaacca ttaaatcgtt 840
tacggataaa gtacaatgtt tggaatggca cgctttagaa gctcaaacgc ttttagcagg 900
aggttgcgat agttctataa gagtatttga ctgtagaact ccagaagctc atcaaacttg 960
gctcttagat ggcgaagctg aacggatatg ttggaatcca ttggaacctt ttagtttttt 1020
ggctggtacc agtaaaggtt cagtccaatg tttcgactgc agaaaagggg aactgtggtc 1080
tatttcagct cacagtaaag aagttagtgg cctatgtgcc agtacacaat gcccaggtct 1140
actaataacg tcttctactg atgaaacagt gaaaatatgg gattataaag acctagcaaa 1200
tgctccttgc ctaataaatg aaaaggaatt taacttgggt aatgtgcact gcttagatct 1260
ttgccctgat ttacccttcg ttatatcagc tggtggagat aaaaagtcgc ataattttac 1320
agtgtttgat atacagaata ttgatgttgt tagaaacaca tttcaaccga gaggcttaat 1380
taaatcagaa tctgttaaag aagaaggtgc ttctacctca acttagagat atgcac 1436
<210> 220
<211> 1420
<212> DNA
<213> 萝卜菜跳甲
<400> 220
aattaacctc aatttcaaac caaaaacact acacgtgaaa gaaaacaagt atttcaattt 60
cacgatggaa atagacgcac agacagaatc aaacataaat ttcataccat gtataaaatg 120
ggtaagaaaa ggcatagcta atacgaatcc cgtgaaactc caactgtcga aaaacgagct 180
agtcgatata ataaatgaaa cgaaagtgaa gctccaagaa gctgaagaaa acgatgatga 240
aattatggaa ggcgatgagc cgaggcaaga tgaattcaat ctagaacagt acgatgaaga 300
tgaaggcgac gagaacactg cgaatgcttt aggcataggc tcgatagctg aattggacaa 360
tgcagccgag gataatttct cagagtctga cgattctgat aaagaagatg aagtcattaa 420
aaccaccgat aatctactat tggtggcgca tgtagaagga gatgcgagca ttttggaggt 480
gtacgtttac aatgaagaag aagaatcttt gtatgtacat cacgacatta tcttaccatc 540
gtttccactt tgtctagaac acttgaatta tgaacccaaa atgcctaagg gtaactactg 600
tgcaatagga tccatgtcac ctattataga aatttgggat ttagatatta tcaatgtaat 660
tgagcctagt tttaccttag gtcaagtaag gactaagaaa agcaacaaat ctatagggca 720
cacagatgca gttttagcat tagcttggaa caaaacattc gaccatataa tggcaagtgg 780
atcagtagac caaacaattc atctatggga catggaaatt aaaaaaccca gcacgaccat 840
caagtcgttt acagataaag tacaatgttt agaatggcac tcgttcgaag ctcagacgct 900
tttagcagga ggttgcgata attctataag agtatttgac tgtagaactc cagaagccca 960
tcaaacgtgg ctcttagacg gtgaagctga gagaatatgt tggaatccgt tagaaccttt 1020
cagttttttg gctggtacca gcaaaggttc agttcaatgt ttcgattgca ggaaaggaga 1080
actgtggtct ctttcagctc acagtaaaga agttactggc ctatgtgcta gcaaacaatg 1140
cccaggtttg ctaataacat cgtctactga tgaaacagtg aaaatatggg attataagga 1200
cttaacaaat gctccttgct taataaatga aaaggaattt aacttgggta atgttcactg 1260
tttggatctt tgccctgatt taccctttgt tatatcagct ggtggtgaca ataagtcgca 1320
taattttaca gtatatgata tacagaatat tgatgttgtt agaaacacat ttcaaccgag 1380
gggcttaatt aaatcagaat ctgttaaaga agaaggtgcc 1420
<210> 221
<211> 1282
<212> DNA
<213> 马铃薯甲虫
<400> 221
ttttttttaa gtttcgtcaa aacatggaag aaaattcgca aagtaacata aattttgtac 60
cctgtgttcg ttgggtgaaa cgaggagtag caaatgcaaa tccagcaaag gttcagctat 120
caaaaaatga gttgatacag ataataaatg atacaaaaaa caaactgaat gtggctgata 180
atgaagacga agtcatggaa ggagaaccgt caggaacaga cgagttcaat tttgaaaatt 240
atgatgaaaa tgacgaggct agcactgcaa atgcacttgg gatcgcatcc ttagccgatt 300
tacctaccgg agcagaagat aatttctccg agtcagatga ttccgagaaa gaagatgatt 360
taatcaagcc tacagataat ctaattctag tgggacatgt tgaaggagat tccagtattc 420
tggaagtgta cgtatacaat gaagatgaat gttctctgta tgtccatcac gatatcgtct 480
tgccgtcctt tcccttatgc cttgagtggc tgaactatga gccaaacatg ccggcaggca 540
attattgtgc agtaggtacg atgtctccaa tcatagaagt gtgggacatg gatatgatga 600
acgtaattga accatcgttt actcttggaa aagcaggcag caagaagaaa aaccgcaaga 660
ggataggtca cactgatgct gtcctttctt tggcctggaa tcatacctac gatcatgtca 720
tggcaagtgg atcagtagac aaaagcataa tcctgtggga catggaacag aaagtgccca 780
gcacaactat taacgttttt ggggacaagg tgcagtgcct cgaatggcac aggatggaag 840
cccaaactct tttggcaggt ggttgcgata gcactgcaag agtctttgac tgccgaactc 900
cagaacctca tcaaatttgg caattagatg gagaagcaga aaggcttatg tggaatcctt 960
tggagcctta cgtgtttcta gctggtacca gcaacggctc tatccaaagc ttcgattgta 1020
gaaaaggtca actttggtca attaatgctc attctaaaga agtaactggt ctggcgatca 1080
gtcgtcagtg tccgggtctt ttggtgaccg cttctcccga tgaaatggtc aagacttggg 1140
attttgcatc aggaagcgtg ccgaagcttg tacacgaaag ggagttcaaa ctgggtaaca 1200
tccactgttt ggaactttgc cccgattccc cattcgttat aactctagga ggggataata 1260
agtcgaacaa tttcgccgtt tt 1282
<210> 222
<211> 1353
<212> DNA
<213> 赤拟谷盗
<400> 222
atggaagaaa acgacaccga aaatcgagtt aatttcatca cttgcgtcaa gtgggtgaag 60
aaaggagtgg caaaaagcca acccgaaaag gtccaattgt ccaaagccga gctcgtacaa 120
gtcataaaag ccacaaaaaa gaaacttcga gtcacaaatg aggaaaatgc tcaaggtgaa 180
tcatcggctc aagatgagtt caatttggaa aattatgacg aacaagacga aggcaccgct 240
gaggctttgg ggataagttc actggctgat ttacaaaata atgaggaaga aaacttctct 300
gaatcagacg attcggacaa agaagacgaa gttatcaaac ctaacgataa tttaattcta 360
gttggacatg tggaaggtga cgcaagttcg ctagaagtgt acatttacaa cgaggaggaa 420
gaatcacttt acgtccacca cgatatctta cttcccgcct ttccactttg tttcgaatgg 480
ttggactacg aacccaacgc cccaaaaggc agttactgtg ctataggttc catgaccccc 540
attatccaag tctgggactt ggacataatt aactgcatag aacccgcgtt taatttaggc 600
cgagccgcaa gtattaaaaa aaaccgacct cacgtcggcc acaaagacgc agttttaact 660
ttggcttgga acaaaaccta cgaacacgtc ttagctagcg gttcagttga caaaactata 720
cttttgtggg acttggagaa caaaactcca agtacgacca tttccgcgtt caaagacaag 780
gtccagtgca tgcagtggca caaactggaa gcgcaaactt tactagctgg gtctagcgac 840
aagaaagcaa aaattttcga ttgtcgcaac cctgagacac atcagacgtg gaaaataaat 900
ggggaggttg aaactctggt ctggaacccg ctacaaccgt tttcattttt cgccggtagt 960
gataccggaa atttgcaata ttttgattgt agaaaaggta gtcaagtgtg ggctgttgaa 1020
gcccatgaaa aggaagtcac tgggttggtt gtgagtccgc aatgtcccgg tttattagtc 1080
acatcgtcgc cagatgggac gataaaaatt tgggactaca ccgaaaatga agccactttc 1140
gtgttcgaaa aagattttag tttagggaca gtccagtgcc tggatttgtc gcccgatttg 1200
ccgtttgtca tagcggcagg aggggataat aaatctaata attttctagt tcacgatttg 1260
agaaacatcg atgttgtaaa acacaagttt ggagaccggc aattagaaga attagtcgca 1320
gaaagtacag aagaaagcat gaacactgat tag 1353
<210> 223
<211> 1435
<212> DNA
<213> 墨西哥豆瓢虫
<400> 223
tataactaaa agtagcaata ttgactttga gaattataag gcattgcata aataaataag 60
aggtgatatc aatggaacac gagttggaag aagaattgga atcgcagagt agaattaatt 120
ttataccttg tttaaaatgg gttaaaaaag gtgttgccaa cacaaatcct atgaaggttc 180
aactctcaaa gaatgaattg gtaaaaatta tcaaagatac acaaaaaaag ctagtccttg 240
ccaaaaatga atcaaataga gatggcgacg aagaagcagt tgatgacctc aatctaagtg 300
attatgacaa tgaagatgaa aacactgcta atatacttgg catcggttct ttggcggagt 360
taacaaatga tgcacaagat aatttctcag aatctgatgg ttctgatata gaagatgatt 420
taatacttgt gggacatctt gagggtgata caagcatttt agaagtttat gtctacaatg 480
aggaagaaga gtccttatat gttcatcatg atatattatt accaagtttt ccattatgct 540
tagaatggct agactatgaa acaaattccc ctaaaggcag ctattgtgct ataggttcca 600
tggatcctat aatagaagta tgggatttag atataatcaa ctcagtggaa tcagctttta 660
aattaggaca aatgggtagt cgaaagaagg gtcgacccca tataggacac agggatgcag 720
tattatcttt agcatggaat aggtcattcg agcatatatt agctagtggt tcggcagata 780
aaagtgtaat attgtgggat ttggatagaa aagaacctag tactacaatc aaagcatttg 840
aagataaagt acagtgtcta gattggcata aattagaggc acaatcttta ttagcaggtg 900
cttgtgatgg tcgtgtaaaa gtctttgatt gccgaactcc tgatagtcat ttgacttgga 960
atttagatgg tgaatgtgaa cagctctcct ggaatcccct acagccattt actttcattg 1020
caggcacaag tgtaggttct ttgcaatgct ttgactgtag aaaaggtcag ttatggtcga 1080
ttccagctca tgaaaaggaa gtcaccggac tctttgtgag tggtcaatgc ccaggattaa 1140
ttgtcacatc ttctccagat gaaacagtga aaatctggga ctatagtggg gaaaattctc 1200
ctgaattgat tcacactaat gactttaatt gtggaatgtt acattgcttg gggggttctc 1260
cagattctcc atttgtaata gcttcaggtg gagataagaa gtccaataat ttcttagttt 1320
acgatatacg aaatatagat gctgtaaaac acaggtttga gggcagggaa ttagtacagc 1380
tggttcctac cactccatca gaaactccag aggtcatgca acaagatgat taatg 1435
<210> 224
<211> 1432
<212> DNA
<213> 十二斑褐菌瓢虫
<400> 224
gtatcctgtg taaaatggat agaaaaagga aaagctcgtg cagaaccttt gaaggttaaa 60
atttcgaagg aagaactggt agaaatgatt aaaaatacta aggaacaact cagtctcagt 120
aacttagaaa atcaagaaaa tgaaccatca gatgacgttg atatggagaa tggagatggt 180
aataatgtgg ggttagatct catcgatgac ttatccacga atcctgaaga ttatttgagt 240
gaatctgaag aagaatccga taaagaggat gacatgataa aaaccagtga taatttggta 300
cttatcggta aatttgatgg tgacgcttgt tcgttagagg tccacgttta taacgacgaa 360
gaggtttcgt tttattgtca ccacgatttc ttattgacaa gttttccggt ttgtttcgaa 420
tggttgaatt acgaggcgaa cagtccgttg ggtaactacg ttgcagtcgg tatgatggaa 480
ccggttatcg aggtttataa tttggacgtt ataaacgtta tgacgccttc gtacgaactt 540
ggacgaaaag ctaacaggag gaaaaattta cctcaaatcg gtcataaaag ttcagttctt 600
tccctggctt ggagtagagc gtacgaacac gttctagcta gcggttcttc cgataaaagt 660
attattctgt gggatttgga aaagagggag ccgagtacta cgattaaatc gttcgaagga 720
gacgttcagt gtctcgagtg gcataaagtg gagacgcaat ctttattagc gggtgcatgt 780
gatagtcaag tgaaactttt cgattgtaac agtccgagta gtcatttaac ttggaaatta 840
gacggagaat gcgaaacttt gaattggcat ccgcatgagc cgttcacgtt cttagcaggc 900
accaatgttg ggtctttaca atgtttcgac tgcagaaaag gtctgatctg gtcgttggct 960
gctcacgaga aagaaatcag tggggttttt atcagtagcg aatgcccggg attaatgatt 1020
acgggatctc atgacggcat ggttaaaacg tgggattaca aaggggagga tgtacctgaa 1080
ttggtctatt ccaaagactt caatttagga acaattttat cccttgaagg ttcaccaaac 1140
tcacccttcg taatagcagc aggtggcgac aacaaagata ataattttgt aatatacgac 1200
gttcgtaaca ttgacgttgt gaaacacaag ttcgagaata gggaattgaa aaaattggaa 1260
acttccacac ctgccgagca gacatgaaga gtaattaaaa tgtaattaaa ggagaatgga 1320
atggatataa gagggagtga atgtcattat gatgggcttt catgaaagta gattgagaag 1380
tgaaaattta tatttgttat attttgaata aatatagtta atttaaaaaa aa 1432
<210> 225
<211> 1470
<212> DNA
<213> 棉红铃虫
<400> 225
aaaactcaaa agagtgaagt gagcacgtga aatcaagaaa attcgtgaat ttgcgcgttg 60
tttcctccat aataatggaa gaaaattcgc ccacggtcag cttgatttca tgcatgcatt 120
ttgtacgccg tggcgtagcg aaatcggtgc cagaaaagat tgagcttaca gaaaaagagt 180
tagagagcat aattaagcaa actgctgaag atttacggct tactgagaat cctgaggaca 240
gcgaggatga ggaaggagaa gcgggtgcta gtgctgtcag agatccacca gcaaatccta 300
atgatgagtt tgattttgaa aaatatgacg aggaagatgc aagtgttaac ccaatcggga 360
ttggctcagt ggctacccta cctaatttag gtgatttgag tgaaaatgtc caaatcagga 420
ctgaagggcc agatagtgac gaagaagatg acataatcaa gccagatgac aacttgcttc 480
tggtcggcca tgttgagagt gatgctagca tactagaggt atacatattc aacaaagaag 540
agggttcatt ctatgtgcac catgacgtta ttctgccttg gttcccgtta tgcattgaat 600
ggctgagtca tgatccatct gatcctaatc caggtaatct ttgcgctctt ggtggtatgg 660
acccagtcat ccaagtatgg gacttggata ttgaaaactg tctagaacca gcgttcaaac 720
tcgggaaaaa gccgaataaa aagaaaaaga taaaacgggt tggacacaaa gacgctgtat 780
tagatctatc ttggaatagg aacttcacac atgtactagc aagtggttca gcagacaata 840
cagttttgct atgggacctg gatcaagggg cacctcacac gacactcagc tactttgaag 900
ataaagtaca atcgctaacc ttccatccat tagaagctca aacattacta tctgggagtt 960
gcgacgggcg cgcacgagtg accgactgcc ggacaccaga cgcacatcgc gcctggcaac 1020
ttgcagggga gatagagagg gtcacgtggg acaaacagaa tcccttctgt tttgcaatga 1080
gcaacaacga aggcaaggtg tcgtacgtag actgcagaca agacgaacct ctatggacca 1140
taactgcaca tgaaaaagaa gtcacaggtc ttattctcag tgaacaagtg ccagggctaa 1200
tggtcacaat aagcacagat ggcaaacaga agacttggga tatatccaac gctccaccag 1260
tccaggtgag cgagcgaagc ggccgcgtcg gccaagcgtt gtgcgcggcc agctgcccag 1320
acgcaccctt ctccatagcc gtcggcggcg acaacaagga atgctgcatc gagatggtgg 1380
acctcaccat caacgaacaa tttgtccacc ggttcgggtc gcgacccctc gtgcaagtca 1440
ctacagaatc aaatgacgac gcaatggaat 1470
<210> 226
<211> 1361
<212> DNA
<213> 草地贪夜蛾
<400> 226
acagtcagct taatatcatg tatgcacttt gtgcgtcgcg gagtggcgaa agcagtgccg 60
gaaaaaatag aattaacgga gaaagaattg gagaaaatta tcaaacaaac agctgatgat 120
ttgaggatag ctgaagctgg tgatgatgag agtgatgaag aaggagaagc aagtgctgcc 180
ccgagagacc caccagcaga tcctaatgac gagttcaact ttgaaaagta tgacgaagaa 240
gataatatta accctgtcgg tattggtact gtagctacat taccaaattt gggtgactta 300
agtgaagggg tacagataag gacagaaggg ccagacagtg atgaagagga tgacatcatc 360
aaaccaaatg ataatctgct tttagtcggc catgttgaga gcgacgccag cgtcttagaa 420
gtatacattt acaacaagga agaggattcg ttctacgtac accatgatat cattctcccg 480
tggttcccgc tgtgtattga gtggctcagc catgacccct cggatcccaa tcctggcaac 540
ctctgtgccc tcggtggtat ggacccggtt atccaagtgt gggacttgga catcgagaac 600
tgtctggagc cagccttcaa gttgggtaaa aaacctaata aaaagaagaa aactaaacgg 660
gtcggacaca aggatgctgt tctagatcta tcgtggaaca ggaactttac acatgtgcta 720
gcgagtggat cagctgacaa cacagtgtta ttatgggacc tggatcaagg cacgccacac 780
accaaaatcg actgcttcca ggataaggta caatccctag cattccaccc gctggaagcg 840
cagacgttgg tgagtggttc gtgcgacggg tacgcgcgcg tgtcggactg ccgcgcgccc 900
gacgcgcacc gcgcatggaa cctcgggccc gagatagagc gggtcgtctg gaactcacag 960
aatccgttct gttttgctat gagtaacaac cagggtaagg tagcgtacgt ggactgccgg 1020
aacgacgcgc cgctgtggac ggtcgacgcg cacgagaagg aggtcaccgg cctcatactc 1080
agcgaccgag tgcccggcct catggtcacc gtcagcaccg acgagaaact caagacttgg 1140
gacatttctg gtgcatcacc gcaacaagta agcgagcgca catgtcgcgt gggccaggct 1200
ctgtgcgccg cgctgtgtcc ggacgcggcg tacagcgtcg ccgtcggcgg ggacaacaaa 1260
cagaactaca tcgaactcgt cgacctcacc attagtgaac aatttgaaag tcggttcgca 1320
tcgcgacccc tgctcatacc acccactgcg cctagtgaag c 1361
<210> 227
<211> 1482
<212> DNA
<213> 欧洲玉米螟
<400> 227
ctcgtgtttc tcagcagaat cgcatgtttt tttgctagta ttttagattt tatttataag 60
tgaattacat aaaaatggaa gaaaacacac ctacagttag cttagtatca tgtatgcatt 120
ttgttaggcg aggtgtggct aaagcggttc ccgaaaagat tgaactaacg gaaaaagaat 180
tagagaaaat aatcaaacag accgcaaatg atttaagatt aacggaagcc ggagacgatg 240
tcagcgacga agaaggcgaa agttctcgcg gcccaccaga cccgcccgct aatcccaatg 300
atgaatttga ctttgaacac tatgaccaag acgacacaag taatccagtt ggtattggct 360
cagtggcaac attacccaat ttaggtgact taagtgaaaa tgttcaaatt aggacggaag 420
gaccagacag tgatgaggag gatgacatca tcaaagcaga cgataacctg cttctagttg 480
gtcacgttga aagcgatgcc agcgtactgg aagtatacat tttcaacaaa gaggaagact 540
cgttttatgt ccaccacgac atcattcttc catggttccc actgtgcatt gaatggctga 600
gccatgaccc ttcagaccct aacccaggca acctctgtgc cctcgggggc atggacccag 660
tgatccaggt gtgggacctc gacatcgaga actgtttgga gccggccttc aagctcggca 720
agaagcccaa caagaagaaa aagacgaagc gcgtgggaca caaggacgct gtgcttgatc 780
tgtcttggaa taggaatttt tcacatgtgc tggcaagtgg gtctgcagac aacacagtgc 840
tcctctggga cctggaccaa gggtcgccgc acacaaaact aaactacttc gaagataagg 900
tgcaatcggt gtcattccac cctctagaag cgcagacgtt gttgacgggc gcgtgcgacg 960
ggcgcgcgcg cgtcaccgac tgccggacgc cggacgcgcg ccgcgcctgg cagctgccgc 1020
cggagatcga gcgcgccgtg tgggacacgc agaacccctt ctgcttcgcc atgagcaaca 1080
accaaggaaa agtagcgtac gtggattgta gacaggatga gccgctatgg accatagatg 1140
cacacgaaaa agaaatcaca ggtctaatac taagcgacaa agttcccggc ttgatggtca 1200
ctgtcagtac agatggaaaa atgaaatcgt gggacataac tagcggcgca tgcgtgcaag 1260
tgagcgagcg ttcgggccgc gtgggacagg cgttatgcgc tgcggcgtgt cccgacgcgc 1320
ccttctctct ggctgtgggc ggggacaata aggagtgctt catagaggtc gtcgacctgc 1380
tggccagcga cacagtttca aatcgcttcg ggtcgcggcc gctggtgtct ataacggtgg 1440
aatcaaacga taacgctatg gaagcataga atataatttt gt 1482
<210> 228
<211> 1604
<212> DNA
<213> 烟草天蛾
<400> 228
atagaaaaaa gtcataaatc tgtaacttcc aaacttagtc acctatgatt atactgtctg 60
tgcatcattc attcaatcat tgttctagtt ttaaatttgt ttatggtctt tatcagagtc 120
tttatcattt gtcaatgtta tgttaacgtg ttttgtctgg gttatgtgca tgtgtttata 180
aaataaatat atacaaatta aactagaaaa tggaagaaaa taccccaaca gtaagcttag 240
tatcatgcat gcgttttgtt cgccgtggta tagctaaagc tgtaccagaa aagattgaat 300
tgaccgagaa agaattggaa aagattatta aacagactgc tagtgaatta agaatttcag 360
aacaggggga tgacaatagc gacgaagaag gtgaagaagc tgcaagtgcg cctccagatc 420
ctcccgcaga ccctaatgat gaattcaatt ttgaacagta tgatgaagaa gatacaaata 480
caaatcccat tggaattggg actgtggcca caatgcccaa tttaggagac ctcagtgaac 540
atgtgcagat cagaactgaa ggcccagaca gtgatgaaga agatgacatt atcaagccta 600
cagacaacct gttgctagtg ggacatgttg agagtgatgc cagcatactc gaagtataca 660
ttttcaacaa ggaagaagga tcattttatg tccatcatga tatcattctg ccctggttcc 720
ctctatgtat cgagtggttg agccatgacc cttcagaccc aaatcctggc aacctttgtg 780
ctctcggcgg aatggaccca gtgatacaag tgtgggattt agatatagaa aattgtctgg 840
agccagcttt caaactcggg aaaaaaccga acaaaaagaa aaaaacgaaa agagtaggcc 900
ataaagacgc agtgcttgac ttatcatgga atagaaactt cacacatgtg ttagcaagtg 960
gatcggcaga caacaccgtg ttattatggg atctggacca aggcactcca cacaccaaac 1020
ttgactactt ccaagacaag gtgcaatcgg tgtcgttcca cccgctggag gcgcagacgc 1080
tgctgtcggg cgcgtgcgac ggctcggcgc gcgtgacgga ctgccgcgcc gccgccgcgc 1140
accgcgcctg gccgctcggg cccgagatcg agcgcgccgt gtgggacacg cacaacccat 1200
tctgcttcgt gatgagcaac aacgagggta aggtggcata tgtcgactgc aggcaaaacg 1260
aaccgctatg gacaatgaca gcacacgaaa aagaagtaac aggtctctta ttaagtgaac 1320
atatacctgg tctaatggtt accgtcagca cagacggcaa aatgaaaact tgggatataa 1380
cagggtctgg cgctactcag gtgagcgagc gcgcgggccg cgtgggccag gcgctgtgcg 1440
cggcgctgtg tcccgacgcg ccgcactcgc tggccgtcgg cggcgacaac aaggagaact 1500
tcatcgaagt cgtcgacctc accattagcg aacagtattt gaatcgtttc ggaagtcggc 1560
agccagtatc catcactaca gaagacaact ctatggaaac atag 1604
<210> 229
<211> 1291
<212> DNA
<213> 玉米穗虫
<400> 229
ttttgatctc atgtgcacat tgtttttgta catgtgatcg aagataaata catatattta 60
agttataaat tttgctatta caatggacga aacaacgcca acagttagtt taatatcatg 120
catgcacttc gttagacggg gtgtggctaa agcagttcca gaaaagatag agttaacgga 180
gaaagagttg gagaagatca ttaaacagac tgctgatgat ttgaggataa cagaggccgg 240
tgaagatcag tcggatgatg aagacgaggc tgctgcagcc cctagagatc caccggccga 300
ccccaacgat gagttcaatt ttgaaaaata cgacgaagag gatgatgtaa accctgttgg 360
catcggtaca gtggctactg taccaaactt aggtgatcta agtgaaaatg tacagataaa 420
cacagaaggg cccgacagtg atgaagaaga tgacattatc aagcctaccg acaacctgct 480
tctggtcgga catgtagaga gtgatgccag tatcttggaa gtatatattt ataacaagga 540
agaggattca ttctatgtac accatgacat catcctgcca tggttcccgc tgtgtgtcga 600
atggctcagc catgacccaa cagatcctaa tcctggcaac ctatgtgccc tcggcggcat 660
ggacccagtc atccaagtat gggacttgga catagagaac tgcttggagc cagcgttcaa 720
gctcggcaag aaacctaata aaaagaagaa gaccaaacgg gttggacaca aggatgctgt 780
gcttgatatg tcttggaata ggaattttac acatgtgcta gcaagtggat cagctgacaa 840
cacagtcctc ttatgggacc tggatcaagg cacaccacac actaaaataa aatgttttga 900
agataaggta caatcagtat cgttccaccc gttcgaggcg cagacgttgc tctcgggcgc 960
ctgcgacggg tcggcccgcg tgtcggactg ccgcagcccc gacgcacacc gagcgtggtc 1020
gctggcgccc gagatagagc gggtcacttg gaacgtgcag aatcctttct gttttgctat 1080
gagcaacaac acaggcaagg tagcatacgt ggattgcaga caggactcac ctctgtggac 1140
actggatgct cacgagaagg aagtcacggg actgatactg agcgagtcag tccccggact 1200
catggtcaca ctcagcactg atcagaagat gaagacgtgg gatatttctg gagcggcccc 1260
ggtggcggtg agcgagcgcg tggtgcgcgc g 1291
<210> 230
<211> 2976
<212> DNA
<213> 筛豆龟蝽
<400> 230
ggttactttt gaaaaggtta tttatgtttg ttataaatat taggtccatt tattcattac 60
taaaaatatt ttttttatga aataccttgg aacttgaagt ttcttatcaa attctttttt 120
tggagggtgt ctccgaattt aatgtttctt atatggacat aaagattatt ataaaatgga 180
agtagaaact gaaagacgtg tccttgctaa atttaagtct gatagggggg aggaaattgg 240
tgcgttatat gacctaggaa tttctgtcaa tgttgagcaa ttgacttaca tttgtaatag 300
tctattggag caggaggact ctgctcagta caccttttat gtgcatgatc aagaaatagt 360
aaacacttta gagggtgtct tggacgtaca gaaactcaac actgaaaatg ttgtcgaaat 420
tatttatcag cagcaagctg tttttaaagt tagagccatt accagatgta caagttctat 480
gccaggacat gcagaagctg taatttcagt caaattcagt cctaatagta gacatttagc 540
aagtggatca ggtgatacca ccgtaagatt ttgggattta actactcaaa cacctctgta 600
cagttgtaaa ggccattcta gctgggttct ttgcatctcc tggtcacctg attctaaaaa 660
gctcatatca gggtgtaaga atggaattat tatattatgg gacccaatat ctggaaaaca 720
aaagggtcca ttaatgaaag gacataagca atggattaca tcattagtct gggagccttt 780
tcacttaaat gcagaatgca gatattttgc cagtagctcg aaagatggtg atatcagaat 840
atgggatgca cctgtgggaa aatgtttacg agttctgact agtcattcaa aaagtgtaac 900
atctttaatt tggggagggt cttctcttct ctattctggc tcacaagatc gaacaattaa 960
agtttggaga cctaacgatg gtgtcctctg tcgaacactg gaaggacatg gacattgggt 1020
taacagtctt tgtgtgaaca ctggctatgt tttaagtttg ggatcatctt tcaggccatc 1080
tgaaaatatt aacaaaaatg atgtcaaagc attgcaaaat gctgctcttg aacggtacaa 1140
agaagttatc ggttcagaag gagagattct tgtgtctgga agtgatgatt ttactatgtt 1200
tatgtggaag cctgaaacaa ataaaaagtc ttttgaaaga ttgactggtc atcaacaact 1260
gattaatgat gtaaaatttt ctcctgatac aaggattcta gcttcagctt catttgataa 1320
gtctataaag ctttgggatg gaaaaactgg gaaattcatt accaccctac gtggacacgt 1380
gcagtcagtt tatatgttgg catggtcttc tgactcaagg ttgttagttt ctgcaagtgc 1440
tgattcaact ttaaaagtat ggaatatgaa aactaaaaaa cttctgaatg atctgccagg 1500
acatgtagat caagtttttg ctgtggattg gtctgctgat ggtgaaaagg tcgcatcagg 1560
cggcaaagat aaactgttga aactatggca gtattaagaa tgtcttccag gataacatag 1620
gttcatagac ttcaagttct ctcaatgtat atatttttta tatactttaa cttttagcta 1680
cttttaaaat gttaatatat actattattg agttttgttt attttttttt taaaaacgac 1740
acaaatgttt tttagtcacc caaggggttg gaaaaaactt ccaatcaaat atttatttta 1800
tatacaatat ctatacaaag ttgtctaata taaattatca gaaataaatt tgttatattt 1860
tgacaatgta tcaaataaat gctggggctt tggtgccgat aggggatcga caaactggac 1920
agttttcttt catttcttct gagcagtctt cacacaaaca aacatgacca catggtaaaa 1980
taattatttc ttttgggttt tctctgcaag caacacatag ttcgttttct gataactctc 2040
ttctatctct agcatttcgt ctcctttctc ttcttgtttc ttctagtctc ctcctttttt 2100
cttcctcttt cactttaagt tctttcataa cccaccattt tctggccaca atgactccta 2160
gacctaatcc aatgctccca aaaagaagtg acaaccactt ataagtccct tgttgctctt 2220
ctaatttacg tattaaggaa gatatcggca tagtggtaag ataatatgga tatcctgtcg 2280
atggagggga tatttggaga ttaccactag cattgataga aacttctcca acagcgatga 2340
gaggagttcc ctctttcaac aattcttctg ttgtttcaat accccgttgt cttactccat 2400
taaaaaatcc tattatgtgg tccataacac ttagtgtact attctcaaat tgattataga 2460
ctgtgtccaa atccaataac tctgcagaaa caggatcaag gatttcaatt gaaaatttac 2520
ccctctttaa aacaaaaggt accacattat gcgatacatg gataacattt ttctgatctg 2580
cccaaaagcc agaagcattt cgagcaatag catgttccat tatagtcttc ctcaaaagta 2640
cgcctgagta atcatgagag ccagaacttt taactgtttt accaactgct ctgacatctc 2700
cttgatagat tgtatatttc ttttcttcat cagtcaattt tttaatatca ggattcacac 2760
caaggcgctg agcgcgttct aatagattaa tggaccaact gcaatttaaa tacaaccttg 2820
tgcatacccc cacaatgaca gcatcaatgc ctaatgctat aagttcacct aggaaatcca 2880
ttttataaat gcttgtaata ccttttttta ttcattaaaa cctaagtatt atgatccata 2940
aattttgtca ttcgttaatt caaaacaaaa agtagg 2976
<210> 231
<211> 3053
<212> DNA
<213> 稻绿蝽
<400> 231
gggggttagc aaagcacgtg tttggagagt ataaatgctc atgtatttac ttagtttgaa 60
gttgatgttt atgaggtttt aaatcgaatt tgtaaaaaca cttctatttt aagtatttta 120
aattaatgca atatcataat ttatgataga ttggttataa tgttatagat tgattatcac 180
ttttatcaaa cttagtaaat aagtttatat caataattta taagttccat taaaatggaa 240
gtagaaactg aggaaaagcg tgtacttgcc aaatttaagt cagaccgcgg tgaagaagtt 300
ggtgccttat atgaattaga tatcaatgtt aatgttgatc agttgacata catttgtaat 360
agcttactag agcaggataa atctgcccaa tacacgtttt atgtcaatga acaagaaatt 420
gttaagacct tagaaggtgt tctagatgtt cagaaattga atactgaaaa tgtcgttgag 480
attatttacc aacaacaagc aatttttaaa gttagagctg ttacaagatg tacaagctct 540
atgcctggtc atgcagaggc tgtcatttct gtcaaattta gtcctaacag taagcatcta 600
gcgagcggct caggtgatac cacagttaga ttctgggatc tcacaacaca aacaccactg 660
ttctgttgta aaggccacaa caactgggtc ctttgcattg catggtcgcc tgactccaca 720
aaattagttt ctggttgtaa ggatggccac atagttttat gggatcccgc cactggcaag 780
caaaaaggtc ctactatgaa aggtcacaag cagtggataa cctcgctggc atgggaacca 840
tatcacctta atgcagaatg cagatatttt gccagcagtt caaaagatgg tgatatcagg 900
atatgggatg caccgattgg aaaatgcttg agaattctta ctagccattc gaaaagtgtc 960
acttcactga tttggggtgg atcttctctc ctttactctg gttctcaaga ccgtacaatc 1020
aaagtttgga ggccgaaaga tggtatactt tgtcgtacct tggaaggtca tgcacattgg 1080
gtgaatagcc tgtcagtcaa cactgactat gttatgcgat tgggttcatc tttcaggcca 1140
tcagaaaatg tcaatcagaa tgataaggaa gctttaaaga aagcagctgt tgagcgatac 1200
cgtgaagtaa gtggttctga aggagaaatt cttgtatctg gaagtgatga ttttactatg 1260
tttatgtgga aaccagaaac aggaaagaaa tcttttgctc ggttaacggg acatcaacaa 1320
ctcataaatg atgtaaagtt ctcacctgat acaaggctac tcgcatctgc atcatttgac 1380
aagtctataa agctgtggga tggtaaaact ggaaaattta taacaactct tcgaggtcat 1440
gtgcagtcag tctatatgat agcgtggtct gctgattctc ggttacttgt tagtgccagt 1500
gctgattcaa cacttaaagt atggactatg aaaacaaaaa aactattaaa tgatctccca 1560
ggccatgttg atcaaatatt tgctgtcgat tggtcggcag atggtgaaaa agtagcttct 1620
ggtggcaaag ataaactttt gaaactatgg cagtattagt tggccaagaa gactacactt 1680
aaatgtaaat atgttttaag atgtatatac atgtatcgat ggaagaaata ttttttaact 1740
attaacaata aacaatactt cactccaatt gtttttaaat accttacact ttgttccttt 1800
caaagttctt ggtattaata aaagaaatac aactatttaa tcgtctactg aacaaaatac 1860
aatgccccta aaatcgatgc cgaaataaaa tttataatat atatatattt tttttaattc 1920
attcatcata taaaagctgc tgctttgctg acaataggcg atcgacagac tggacaggta 1980
tccttcactc cttcagaaca atcttcgcat aagcagacat ggccacaatg taaaattatt 2040
atttctttgg gattgaccct gcatgcaaca cacaattcat tatctgatag ttcccttcca 2100
tctcgcgcat ttcttctcct ttccctcctt gtttcttcaa gcctcctttt tttttcttct 2160
tcttttaacc tcagttcttt cagagtccac cattttttaa cgacaattac tccaagaacg 2220
actccaatgc ttccaaaaag gagtgtcaac cacctatatg tgctttgttg ttcctctaat 2280
tttcttatca gagatgacac tggcatggtg gttaagtaat aagggtaacc agcagatgga 2340
ggagacatct gaagataacc actgtgatta accattactt ccccgatagc tgtcagtgga 2400
gttccttctt tcaaaagttc ttcagttgtt tcaatacctc tctgtcttac tccattgaaa 2460
aaaccaataa tatgatccat aacacttagc gtactgtttt caaactggtt atacactgta 2520
tccaaatcta gtagctctgc agaaactgga tcaaggatct caatagtgaa tttgcctttc 2580
ttaagtacaa aaggtacaac attatgtgac acgtggataa catttttttg atcagcccaa 2640
aatccagatg cattacgtgc gattgcatgt tccataatac tccttctcag gatcacacca 2700
gaataatctt gtgagccaga acttttaact gattttccca cagctttgac atctccttga 2760
caaatagtgt atttcttttc ctcatcgctt aaatttttta tttcaggatt gattccaagg 2820
cgttgagctc tttctattaa attgatagac caactgcaat tcagatataa cctagtacaa 2880
acacccacaa ttatggtatc aatccctaaa gcgataagtt cccccaagaa atccatcact 2940
tttaattaca ttatttgagt ctcattttaa ttatgaacaa accgataagt taaattttgt 3000
cattgttttc aagttgatgc agtaaggttc agcaaaaaca aagacatgaa aaa 3053
<210> 232
<211> 275
<212> DNA
<213> 玉米根虫
<400> 232
gctccaaagt atggtaccct agtgcctaac agaatattcg tgggaggaat atcagctaac 60
acaacagaag gagaattgat gcaacttttt agcaactatg gtactgttaa agctgctaaa 120
attatacagg acagggctgg tgtgtcaaaa ggatatggtt tcatcacctt tgagagtgaa 180
gatgatgcta aaaggcctct tagagaagct gaaaacatag tgctaagaga aagaaaactt 240
aatatagcac ccgctattaa aaaacagcct tttag 275
<210> 233
<211> 275
<212> DNA
<213> 玉米根虫
<400> 233
tgccagtcaa ttcttttttc ctccgggtgc tgccgtgcca tattttcaga gtggagttac 60
atattatact cagccagctc ctgctgcacc tggagatccc actgctcaac aacctgttta 120
tcaaccccct ccaatgtatc ccacgcaaac tggtcctcca caggcagcta catacccttc 180
tatgatgttt cctgctcaaa ctatatatat gccgcaacag tatcccatgc ctattccgta 240
cgagtacaac ttctaccagg gtaacggacc gtcgc 275
<210> 234
<211> 275
<212> DNA
<213> 玉米根虫
<400> 234
ctcagtatct ggccggaggc caaagcggtc cgggaaactc tcattgcacc tcattaccat 60
catcaaacag tccacccaga cccaattgtt atggtcaaca ggtgcctgcc tataattcag 120
gagatcccgt gtactacaac ttgccaatat acggagccac tttggaaggt ccgccattat 180
acgctgatgc cttcgatcta agcgctagtg gagcttatgc ggaggaaacc tacagcggca 240
tgattactca agaaaatgta gaagcttctc ttgga 275
<210> 235
<211> 275
<212> DNA
<213> 玉米根虫
<400> 235
cggtaaattc aactcgtgca acttccgata attttacgca acatatggat caatctctcg 60
ttgagtctga ttcaaatatt gttaatgttg gaggtaaacc accagcacat aaatcttcag 120
agacaattgt ttctactcct tcccagcctc aggaggagag aaactcacac acgcctattg 180
tttcactact ttccatagat catcaacagg aaaaagacta ttcatcaatg caaagcggtc 240
gtagacggaa acttctgatt caaagcaatc aaaat 275
<210> 236
<211> 275
<212> DNA
<213> 玉米根虫
<400> 236
gtacccatgt atcctacaaa cggatacgtc aaccagtttg tcggatataa tcctcaacaa 60
ccatccccac cttcattcgg tccacctctc tataacaacg gctacggtta ttctgaatat 120
cgtcgattta acggataccc agatctcagg tcaaataata atagtaataa accgaggaga 180
cgaatatacg aaaataggcg gtcgaacgat cattctagcc gttccagtct tcgcacagac 240
tcaaactcat cggctagttg tgttgacgaa aataa 275
<210> 237
<211> 275
<212> DNA
<213> 玉米根虫
<400> 237
cgaagaaaaa acttttcgaa ctactgttaa cactcctcca cctgccccat actcacctat 60
gaccaatcat caattcaaat ttattaatta cactaacacg aaaaacgttt ataatcataa 120
gtacgctcac acgtttataa tcataagtac gctaacaaca ataattatta taatagaaca 180
gctacacaca atgaccgatt taaacatacc gaacctttag cgaataaaaa caataacagt 240
tccatttacc cattaacaaa taatttccta aataa 275
<210> 238
<211> 373
<212> DNA
<213> 玉米根虫
<400> 238
tagccatacc caaagtaatt ctgattccct tccctcccaa aattcccagg tacatgtgac 60
gactacgggt cagagcttcg tcccggcggc tatccaagct cagacgaaac gcaacaagcg 120
ttctttgaga agaagcggtg ccagtgccgg aggcatcaac gaaatcggcg ccggtgacgc 180
ccctttacca ggcgaagaat gcgaagatgt ctacaagaag ctggaaacgc ttaagttgta 240
acgaaaaagt ggtatctttt cttgtatatt tttttctatt tatttaagac tgtacaataa 300
tgtaaaatac tagaaattta atgtaaacta aagaggaaaa atatataata catatttatt 360
gaagatgaaa aaa 373
<210> 239
<211> 381
<212> DNA
<213> 玉米根虫
<400> 239
cgtttagtag tacatagaga aatcgctttt agttttaatc ttttctgtgt tggaatattg 60
aacaaattct aatgattcgt aaaataacac ttctgttaaa aaccaaaact gatagtttgt 120
tcaaaaaaag tacaattttt ctgggcttgc tactgaaata atgtgccata ctctaattgt 180
ggaacaaatt gtaggtatat gttgaaagtg aacaaataac taatgcttac aataatgtcg 240
acatcaagtc aaaataatgg taaaaagagt tcaagcagct ctattgttaa caccccttcc 300
agtactccca ttaccaatct tgcaaatggt agtagtagta cccaaggaac agcacatagt 360
tcacctccac ctaataacaa t 381
<210> 240
<211> 271
<212> DNA
<213> 玉米根虫
<400> 240
tgcccaaatc aaataaatct agaaatgcgt tttactactt tatggaggat tttaaacaaa 60
gacaaggatt tgcttgtaaa tcgatgaagg atgtagcaga tgctgcaggt ccccattggg 120
ctaggatgtc taaagcagaa agaagacctt atgaagagag ggcaaacaaa gagaagggag 180
gtttgagata tacttgggat ggtcaatgcg ttgaagatat agaaaggagg aacaaagctg 240
aggacaataa aattgctaag atgaaagatg a 271
<210> 241
<211> 275
<212> DNA
<213> 玉米根虫
<400> 241
cagtgggaga gcgaatctgt agtgtcagac tcctctacag ctactttcaa taatagcttt 60
ccaggtctga aatcgaaatg gacattaagc aattctcaac ctgtagaaac tgactcaatg 120
agccaaatgt caggtcagag ctatgcaagt gctatgggaa ctagaagaca gttttctagt 180
gcaatgggaa atagaggatc tcaaagagag tcctctaaca attccaacaa tggtgacact 240
agtgattcga ataccgatta ttttaacagt caatc 275
<210> 242
<211> 500
<212> DNA
<213> 玉米根虫
<400> 242
tatcgactgc accttgaaat ttgccaaaga aaattgtcga ctccgagatg aaatgttctt 60
cataatccat atcaatactt tctgttacca tgccagtggt gatcggtatt atcccgctga 120
aattgccatt tcatgcttca gcttggagga tggcgttttg cctcaaaatg tgtttcataa 180
aattattaag ccaggtagac taccactggg gtatgctagt gacgcaaaaa agcaatctga 240
agattcacat caactgccta tccccttaac tggtgacgac gacgacaatg tggaagaagt 300
ttatacagag atgaaagaat ttatattatc taaaaccgga gatactaaga aattccctcc 360
cttgtatgct aagaaagata ttgttaaaat gttgcaacat gtgttagaca cttggtgcgc 420
tgattttgat gagccgccat tatttaaagt ttattattta caatatatgt ttcaagtatt 480
aaaaaactcg gtagctaacg 500
<210> 243
<211> 350
<212> DNA
<213> 玉米根虫
<400> 243
ataacgtatg gcccttatat tcaatggcag aaacggaact ggataaggat ctttattccc 60
atactgttgg aatatgttgt gaattccatt ctgttagtga agcttgtcaa ttttgcagca 120
agtcagtggc agtacgactt gcatacacaa tttgtgataa ttgctgttcg ttcctagata 180
ttcctataga gcctggaaga cacgtacctg atcaagcttt tacagcatcc agtttgagct 240
caagagcttc atcaaaagct agtttgtata caggttccag taaatcttct aagaagcagt 300
cagttcgtga agatgacaaa gacactgttt tatcgttttc tagtgcctct 350
<210> 244
<211> 500
<212> DNA
<213> 玉米根虫
<400> 244
atttccggct cttggaaggg gaagaggtat ggccagaaat cgaagaaatt gatcattttt 60
gactgcaatg ggtcttgttc cacttcatga gattgttaaa tgagtatgta actgattaca 120
ttaggcgaca atattttttt tagtgtcaga ttatttgatg taattgtcct tttgacaatt 180
ctttaatcat tgatgttttc ttctattgtg gtgtttttag atgtcatatt tttatgtatg 240
taaattggtt ttttatctaa gtatagaata ttacatttta gtttaataag ataccagtaa 300
agtatattgg tttaccagtc gcctaatgta aactagtata tacataccta tattattttt 360
ctttaatagt tttaagtcaa ttttgataca gatcacgaga cagatatttt ttccgtctag 420
tccgaattgc gttccgtttc ttgggccttg tcgaattgcg tcccaaatat tttgcttacc 480
tggccaccga taacgtctag 500
<210> 245
<211> 500
<212> DNA
<213> 玉米根虫
<400> 245
tccgaattgc gtttcatttc ttaattagcc aacccaattg cggtcagaag atagcttaat 60
aaatattagt taagcaggga aatacaatca acgtaatatc aactaacagt ttatttttat 120
tttagcaccg aatttataac taacacattt aacgaaattg tatcttgaaa tttcacccct 180
gttatactgg ttaattttct gttcgagaaa aatatatttg ctggaggtag ctttatctac 240
atttctagaa atattttgtt taacagaatt tatttttata taagaatcgg tttgaatgcg 300
ctttaatgtt tcaacaaaca aaaaaatatt aggatgtgga gacaaacaat aatttttaaa 360
ttttgaatga aatcactcac aagaattagt tgttctatat atagatgcgc tattctttgc 420
ccaaatttgt ggaggaaatg tgtagtcctc ttcgatatac ctttcaacta gatagtttgc 480
aaatgcgtca actcgatgat 500
<210> 246
<211> 500
<212> DNA
<213> 玉米根虫
<400> 246
catctggttt ttcaggcata aaatcttcca caaaacaatc tccaacttgg ctcggaggaa 60
gatacattag gccaaaaata tgcttcagcc atttacctat ttctgagttc tcgttcttat 120
actcagaact caaccccata ttttgtactt tacgccacct gtaacaagat tatagaataa 180
attctttatt tcagaaagta cttgcattca agaaaaagtt tgaaacaggt cagattttat 240
aaaaaaaata catctttaaa aaataatgag aatatttagg agccaatttt ttgacagagg 300
ttatgacgac atcttttttt tatgtcaaaa atttgaagat attttaccta caattcttgt 360
tttaacttat atcaaatgct gttttttgta aaaaaaaatt gaaaaaagaa attacagtga 420
agtttgaaat aaacatattg aaatgtttag ttttacagca aaaaaaatag gatgacgtca 480
taactcaagt ttttcttaac 500
<210> 247
<211> 500
<212> DNA
<213> 玉米根虫
<400> 247
aatctactat ttgccagtta cattgccata cgtattactt accaagcttg agcaagatga 60
agtcgataac caacaacatt tgcttccgga aaaacttctc gtacgctgga gtggatacct 120
aattcgtaat caatcactat tttcgttgga tttaaattgc atcccaaatc ttgacatttt 180
ttctttaata attcgaataa tgatttgtaa gtgaattttt gcttattagg taacaaacaa 240
aatgcaaccg gaacataagt gtcattaatc aatgtatgta atgtaaacat ttaaaaaaaa 300
attgtgcaat aatcgaatgt tccatccata aataaagtgg aattgttaca cataaatgaa 360
agattagaca ttcatgaaaa aacaaaaata ttaaactgtt catcattaat caacaaaaac 420
tgttcatttt tgatagtaaa tatttcaatt tgttccaaaa attcttgaac atccttggct 480
gattttggca attttggaac 500
<210> 248
<211> 322
<212> DNA
<213> 玉米根虫
<400> 248
cgttttcaat cgcgtgcgat taatgttctt tttttatgta ttggacatct tttgttgaca 60
gagaggtaag tacttcgctt tgcttttcga tttccttgtg taacaacttc gcaggtcgtt 120
ccgcaatatc ttctgttgct tttcttttaa gagaattgtt aactttctgg cgaattaata 180
cttcttcagc aagctcatga ttgtgttcac cactaacttt tgaaaacaca ttatttaatg 240
tatatagttt tgctttacac tttttgaagt tatacttctt taggcgcgat tgagtaatta 300
tttattatta atctgcgcgc at 322
<210> 249
<211> 272
<212> DNA
<213> 玉米根虫
<400> 249
tggagtacga aaatacacaa caaaatataa acttcgtacc gtgtgtaaga tgggttaaac 60
gaggagtggc caattcaagc ccagtaaaat tgcaactgtc gaaaaacgag ctggctcaaa 120
ttattaatga caccaagatt aaattacaag aatccaatga aaatgaagat gagcctatgg 180
aagaaggtga aacgtctcaa acagatgagt ttgccttaga ggattacgat aaagaagacg 240
aaaatgagga cactgcaaat gctttaggaa tt 272
<210> 250
<211> 275
<212> DNA
<213> 玉米根虫
<400> 250
tggaaatcaa aaaaccaagt acaaccatta aatccttcca agaaaaagtt caatgtctcg 60
aatggcatcc tttagaggca caaacacttt taggtggagg ttgtgacaaa tctgcaagag 120
tatttgatgg caggacccct gaaacccacc aaacttggct acttgatgga gaagctgaaa 180
ggctatgctg gaacccatta gaacctttca catttttagc aggtaccagc agtgggtctg 240
tacagtgttt cgactgtaga aaaggacagc tatgg 275
<210> 251
<211> 248
<212> DNA
<213> 玉米根虫
<400> 251
caccggctta cgtaataatt aatttattcc tttcatttgc accatactat atattattta 60
ttgagtggac aaatgttaaa aagtaagtct ggcagcgaac gtgttaatga aacctagtaa 120
aaggctccaa catttgacac ttcttcatgc aacatgttct gttctgtgtc aatctaacct 180
aacctaacat tttcaaatac gaatatggaa acatttcacc acatcaacac gtgaattaat 240
attcgaaa 248
<210> 252
<211> 272
<212> DNA
<213> 玉米根虫
<400> 252
tcctctgtgt ttagaaccgc taaactatga accgaagatg cccaaaggaa attattgtgc 60
agtgggatca atgtcacctg ttatagaggt ctgggatgta gatattatga atgttattga 120
accttcattt actttaggga gacctgctag taaaaagaag aacaaggaac atataggcca 180
tacggatgca gtgctttcat tagcttggaa caaaaccttt gaacatgtgt tggctagtgg 240
atctgtagat caaacaatac acttgtggga ta 272
<210> 253
<211> 486
<212> DNA
<213> 玉米根虫
<400> 253
tcagttaaag cacacagcaa ggaagtaaca gggttagtcc ttagcaaaca atgccaagga 60
ttgttgatta cttcttctac agatgaaaca gttaaaattt gggacttggc tacactggaa 120
gctgaaccta agcttgttaa tgaaaaggag tttaatatgg ggaatattca ctgtttggat 180
ttatgtccag acttgccgtt tgttatatct gtaggtggag ataagaagtc aaataatttt 240
actgtttttg atgtacagaa tattgatgtt gttaaacaca catttgggcc aagaggattg 300
gtacagctag ttccagatac agaagaaaat actaatacgt tttattgttt aataaagttt 360
ttattttatt tatttgtaat aatttcatgc ttcttcttta agtaccgtgc ccaaatatta 420
gtcattggta gattccttga caatttgccg atactgtttt cgattactga acaattcatc 480
ttctga 486
<210> 254
<211> 600
<212> DNA
<213> 玉米根虫
<400> 254
ggggctttct gatttttgac agcttctata gaagtttatc aagatgttga tgccaaaaaa 60
gaatagagta tgtatttacg aatacctctt caaagaggga gtcatggtag ctaaaaaaga 120
ttaccatgcc ccaaaacacc tcgaactaga aactatccct aaccttcaag taattaaggc 180
tttacaatca cttaaatcaa aaggttacgt aaaggaacaa ttcgcctgga ggcattatta 240
ttggtatttg actaactctg gcatcgaata cctccgcaca ttcttacact tacctggaga 300
aattgtccca tctaccttga aacgcccagc aaggacagaa accacccgtc ctagaccagc 360
tgctctcaga tctgagacat ctaaaccttc agaagaccgt gcaggataca gaaggactcc 420
tggaggccct ggagctgaca agaaagctga tgttggtcca ggaactggag atgttgagtt 480
caggcaagga ttcggacgtg gacgggcacc acaataaatt tattgataag ttaattttta 540
taaattgatc agccaataaa aagtttggtt aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 600
Claims (110)
1.一种沉默元件,其包含至少一个双链RNA区域,所述双链RNA区域的至少一条链包含与以下互补的多核苷酸:
(a)包含SEQ ID NO:1-53或107-253中任一项的核苷酸序列;或其变体和片段,及其互补序列;
(b)包含与核苷酸SEQ ID NO:1-53或107-253中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者
(c)包含SEQ ID NO:1-53或107-253中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列;
其中所述沉默元件对昆虫植物有害生物具有不育化活性。
2.如权利要求1所述的沉默元件,其中所述沉默元件的至少一条链包含与以下互补的多核苷酸:
(a)包含SEQ ID NO:1-24、33-34、36-51或107-191中任一项的核苷酸序列;或其变体和片段,及其互补序列;
(b)包含与核苷酸SEQ ID NO:1-24、33-34、36-51或107-191中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者
(c)包含SEQ ID NO:1-24、33-34、36-51或107-191中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列。
3.如权利要求2所述的沉默元件,其中所述昆虫植物有害生物为鞘翅目(Coleoptera)植物有害生物。
4.如权利要求3所述的沉默元件,其中所述鞘翅目植物有害生物为叶甲属(Diabrotica)植物有害生物。
5.如权利要求4所述的沉默元件,其中所述叶甲属植物有害生物包含玉米根萤叶甲(D.virgifera virgifera)、墨西哥玉米根虫(D.virgifera zeae)、南美叶甲(D.speciosa)、北方玉米根虫(D.barberi)、墨西哥玉米根虫(D.virgifera zeae)、或斑点黄瓜甲虫(D.undecimpunctata howardi)。
6.如权利要求3所述的沉默元件,其中所述鞘翅目植物有害生物为条跳甲属(Phyllotreta)植物有害生物。
7.如权利要求6所述的沉默元件,其中所述条跳甲属植物有害生物为黄曲条跳甲(Phyllotreta striolata)或十字花科条跳甲(Phyllotreta curciferae)。
8.如权利要求3所述的沉默元件,其中所述鞘翅目植物有害生物为瘦跗叶甲属(Leptinotarsa)植物有害生物。
9.如权利要求8所述的沉默元件,其中瘦跗叶甲属植物有害生物为马铃薯甲虫(Leptinotarsa decemlineata)。
10.如权利要求1所述的沉默元件,其中所述沉默元件的至少一条链包含与以下互补的多核苷酸:
(a)包含SEQ ID NO:25-30、35或192-196中任一项的核苷酸序列;或其变体和片段,及其互补序列;
(b)包含与核苷酸SEQ ID NO:25-30、35或192-196中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者
(c)包含SEQ ID NO:25-30、35或192-196中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列。
11.如权利要求10所述的沉默元件,其中所述昆虫植物有害生物为半翅目(Hemiptera)植物有害生物。
12.如权利要求11所述的沉默元件,其中所述半翅目植物有害生物为无网蚜属(Acyrthosiphon)、小粉虱属(Bemisia)、褐飞虱属(Nilaparvata)或茶翅蝽属(Halyomorpha)植物有害生物。
13.如权利要求12所述的沉默元件,其中所述无网蚜属植物有害生物为豌豆长管蚜(Acyrthosiphon pisum)。
14.如权利要求12所述的沉默元件,其中所述小粉虱属植物有害生物为烟粉虱(Bemisia tabaci)。
15.如权利要求12所述的沉默元件,其中所述褐飞虱属植物有害生物为稻褐飞虱(Nilaparvata lugens)。
16.如权利要求12所述的沉默元件,其中所述茶翅蝽属植物有害生物为茶翅蝽(Halyomorpha halys)。
17.如权利要求1所述的沉默元件,其中所述沉默元件的至少一条链包含与以下互补的多核苷酸:
(a)包含SEQ ID NO:31-32、52-53或197-199中任一项的核苷酸序列;或其变体和片段,及其互补序列;
(b)包含与核苷酸SEQ ID NO:31-32、52-53或197-199中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者
(c)包含SEQ ID NO:31-32、52-53或197-199中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列。
18.如权利要求17所述的沉默元件,其中所述昆虫植物有害生物为鳞翅目(Lepidopteran)植物有害生物。
19.如权利要求18所述的沉默元件,其中鳞翅目植物有害生物为斜纹夜蛾(Spodopteralitura)、玉米螟(Ostrinia nubilalis)、谷实夜蛾(Helicoverpa zea)、或草地贪夜蛾(Spodoptera frugiperda)。
20.如权利要求1-19中任一项所述的沉默元件,其中所述沉默元件包含发夹环。
21.如权利要求1或20所述的沉默元件,其中所述沉默元件包含第一区段、第二区段和第三区段,其中
(a)所述第一区段包含至少约19个核苷酸,这些核苷酸与SEQ ID NO:1-53或107-253中任一项所示的序列具有至少90%序列互补性;或其变体和片段,及互补序列;或者所述第一区段由与SEQ ID NO:1-53或107-253中任一项所示的序列具有至少90%序列互补性的至少19个核苷酸组成;
(b)所述第二区段包含足够长的环,以允许所述沉默元件被转录为发夹RNA;并且,
(c)所述第三区段包含至少约19个核苷酸,这些核苷酸与所述第一区段具有至少85%互补性;
其中所述第二区段与SEQ ID NO:1-53或107-253中任一项所示的序列或所述构建体的其他区段不互补;并且
其中所述第一区段和所述第三区段至少形成部分地双链的区域。
22.如权利要求21所述的沉默元件,其中所述第三区段与所述第一区段具有至少85%互补性。
23.如权利要求21所述的沉默元件,其中所述第三区段与所述第一区段具有至少90%互补性。
24.如权利要求21所述的沉默元件,其中所述第三区段与所述第一区段具有至少95%互补性。
25.如权利要求21所述的沉默元件,其中所述第三区段与所述第一区段具有至少98%互补性。
26.如权利要求21所述的沉默元件,其中所述第一区段与鞘翅目昆虫物种互补;并且其中所述第三区段与不同的鞘翅目昆虫物种互补。
27.如权利要求21所述的沉默元件,其中所述第一区段与半翅目昆虫物种互补;并且其中所述第三区段与不同的半翅目昆虫物种互补。
28.如权利要求21所述的沉默元件,其中所述第一区段与鳞翅目昆虫物种互补;并且其中所述第三区段与不同的鳞翅目昆虫物种互补。
29.一种DNA构建体,其包含编码如权利要求1-28中任一项所述的沉默元件的核苷酸序列。
30.一种表达构建体,其包含如权利要求29所述的DNA构建体。
31.如权利要求30所述的表达盒,其中所述多核苷酸有效地连接至异源启动子。
32.如权利要求30所述的表达盒,其中所述多核苷酸的侧翼是在所述多核苷酸的一个末端处的第一有效地连接的趋同启动子和在所述多核苷酸的对向末端处的第二有效地连接的趋同启动子,其中所述第一趋同启动子和所述第二趋同启动子能够驱动所述沉默元件的表达。
33.一种宿主细胞,其包含如权利要求1-28中任一项所述的沉默元件、如权利要求29所述的DNA构建体、或如权利要求30-32中任一项所述的表达构建体。
34.如权利要求33所述的宿主细胞,其中所述宿主细胞是细菌细胞。
35.如权利要求34所述的宿主细胞,其中所述细菌细胞是灭活的细菌细胞。
36.如权利要求33-35中任一项所述的宿主细胞,其中所述宿主细胞包含如权利要求30所述的表达构建体。
37.如权利要求36所述的宿主细胞,其中所述表达构建体包含与如权利要求29所述的DNA构建体有效地连接的转录启动子。
38.如权利要求37所述的宿主细胞,其中可通过将所述宿主细胞暴露于外源性分子来诱导所述转录启动子。
39.一种组合物,其包含如权利要求1-28中任一项所述的核糖核酸构建体、如权利要求29所述的DNA构建体、如权利要求30-32中任一项所述的表达构建体、或如权利要求33-38中任一项所述的宿主细胞。
40.如权利要求39所述的组合物,其进一步包含农业上可接受的载体。
41.如权利要求39所述的组合物,其进一步包含除草剂化合物、杀昆虫剂、杀真菌剂、杀线虫剂、农业上可接受的载体、和/或细菌,或其组合。
42.如权利要求39或41所述的组合物,其中所述组合物呈液体形式、固体形式或凝胶形式。
43.如权利要求42所述的组合物,其中所述组合物是固体形式。
44.如权利要求43所述的组合物,其中所述固体形式是丸剂、粉末、聚集体或模制物品。
45.一种植物细胞,其基因组中稳定地并入了编码沉默元件的异源多核苷酸,其中所述多核苷酸包含:
(a)包含SEQ ID NO:1-53或107-253中任一项的核苷酸序列;或其变体和片段,及其互补序列;
(b)包含与核苷酸SEQ ID NO:1-53或107-253中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者
(c)包含SEQ ID NO:1-53或107-253中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列;
其中所述沉默元件降低所述植物有害生物的能育性。
46.如权利要求45所述的植物细胞,其中所述植物细胞的至少一条链包含与以下互补的多核苷酸:
(a)包含SEQ ID NO:1-24、33-34、36-51或107-186中任一项的核苷酸序列;或其变体和片段,及其互补序列;
(b)包含与核苷酸SEQ ID NO:1-24、33-34、36-51或107-186中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者
(c)包含SEQ ID NO:1-24、33-34、36-51或107-186中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列。
47.如权利要求46所述的植物细胞,其中所述昆虫植物有害生物为鞘翅目植物有害生物。
48.如权利要求47所述的植物细胞,其中所述鞘翅目植物有害生物是叶甲属植物有害生物。
49.如权利要求48所述的植物细胞,其中所述叶甲属植物有害生物包含玉米根萤叶甲、墨西哥玉米根虫、南美叶甲、北方玉米根虫、墨西哥玉米根虫、或斑点黄瓜甲虫。
50.如权利要求47所述的植物细胞,其中所述鞘翅目植物有害生物是条跳甲属植物有害生物。
51.如权利要求50所述的植物细胞,其中条跳甲属植物有害生物为黄曲条跳甲。
52.如权利要求47所述的植物细胞,其中所述鞘翅目植物有害生物是瘦跗叶甲属植物有害生物。
53.如权利要求52所述的植物细胞,其中瘦跗叶甲属植物有害生物为马铃薯甲虫。
54.如权利要求45所述的植物细胞,其中所述沉默元件的至少一条链包含与以下互补的多核苷酸:
(a)包含SEQ ID NO:25-30或35中任一项的核苷酸序列;或其变体和片段,及其互补序列;
(b)包含与核苷酸SEQ ID NO:25-30或35中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者
(c)包含SEQ ID NO:25-30或35中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列。
55.如权利要求54所述的植物细胞,其中所述昆虫植物有害生物为半翅目植物有害生物。
56.如权利要求55所述的植物细胞,其中所述半翅目植物有害生物为无网蚜属、小粉虱属、褐飞虱属或茶翅蝽属植物有害生物。
57.如权利要求56所述的植物细胞,其中所述无网蚜属植物有害生物为豌豆长管蚜。
58.如权利要求56所述的植物细胞,其中所述小粉虱属植物有害生物为烟粉虱。
59.如权利要求56所述的植物细胞,其中所述褐飞虱属植物有害生物为稻褐飞虱。
60.如权利要求56所述的植物细胞,其中所述茶翅蝽属植物有害生物为茶翅蝽。
61.如权利要求45所述的植物细胞,其中所述沉默元件的至少一条链包含与以下互补的多核苷酸:
(a)包含SEQ ID NO:31-32或52-53中任一项的核苷酸序列;或其变体和片段,及其互补序列;
(b)包含与核苷酸SEQ ID NO:31-32或52-53中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者
(c)包含SEQ ID NO:31-32或52-53中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列。
62.如权利要求61所述的植物细胞,其中所述昆虫植物有害生物为鳞翅目植物有害生物。
63.如权利要求62所述的植物细胞,其中鳞翅目植物有害生物为斜纹夜蛾。
64.如权利要求45所述的植物细胞,其中所述植物细胞包含如权利要求30所述的表达盒。
65.如权利要求45所述的植物细胞,其中所述沉默元件表达双链RNA。
66.如权利要求45所述的植物细胞,其中所述沉默元件表达发夹RNA。
67.如权利要求45所述的植物细胞,其中所述沉默元件有效地连接至异源启动子。
68.如权利要求45所述的植物细胞,其中所述植物细胞来自单子叶植物。
69.如权利要求68所述的植物细胞,其中所述单子叶植物是玉米、大麦、粟、小麦或稻。
70.如权利要求45所述的植物细胞,其中所述植物细胞来自双子叶植物。
71.如权利要求70所述的植物细胞,其中所述双子叶植物是羽衣甘蓝、花椰菜、西兰花、芥菜植物、卷心菜、豌豆、三叶草、苜蓿、蚕豆、番茄、木薯、大豆、卡诺拉油菜(canola)、苜蓿、向日葵、红花、烟草、拟南芥(Arabidopsis)、或棉花。
72.一种植物或植物部分,其包含如权利要求45所述的植物细胞。
73.一种转基因种子,其来自如权利要求72所述的植物。
74.一种用于防治植物昆虫有害生物的方法,其包括向植物昆虫有害生物饲喂包含沉默元件的组合物,其中所述沉默元件防治所述植物有害生物,其中所述沉默元件包含与以下互补的序列:
(a)包含SEQ ID NO:1-53或107-253中任一项的核苷酸序列;或其变体和片段,及其互补序列;
(b)包含与核苷酸SEQ ID NO:1-53或107-253中任一项的至少90%序列同一性的核苷酸序列;或其变体和片段,及其互补序列;或者
(c)包含SEQ ID NO:1-53或107-253中任一项的至少19个连续核苷酸的核苷酸序列;或其变体和片段,及其互补序列;或者
其中所述沉默元件对所述植物有害生物具有昆虫不育化活性。
75.如权利要求74所述的方法,其中所述组合物包含其基因组中稳定地并入了编码所述沉默元件的多核苷酸的植物或植物部分。
76.如权利要求74所述的方法,其中所述沉默元件包含双链RNA。
77.如权利要求74所述的方法,其中所述沉默元件包含发夹RNA。
78.如权利要求75所述的方法,其中编码沉默元件的所述多核苷酸有效地连接至异源启动子。
79.如权利要求74所述的方法,其中编码沉默元件的所述多核苷酸的侧翼是在所述多核苷酸的一个末端处的第一有效地连接的趋同启动子和在所述多核苷酸的对向末端处的第二有效地连接的趋同启动子,其中所述第一趋同启动子和所述第二趋同启动子能够驱动所述沉默元件的表达。
80.如权利要求74所述的方法,其中所述沉默元件包含第一区段、第二区段和第三区段,其中
(a)所述第一区段包含至少约19个核苷酸,这些核苷酸与SEQ ID NO:1-53或107-253中任一项所示的序列具有至少90%序列互补性;或其变体和片段,及互补序列;
(b)所述第二区段包含足够长的环,以允许所述沉默元件被转录为发夹RNA;并且,
(c)所述第三区段包含至少约19个核苷酸,这些核苷酸与所述第一区段具有至少85%互补性;
其中所述第二区段与SEQ ID NO:1-53或107-253中任一项所示的序列或所述构建体的其他区段不互补;并且
其中所述第一区段和所述第三区段至少形成部分地双链的区域。
81.如权利要求80所述的方法,其中所述第三区段与所述第一区段具有至少85%互补性。
82.如权利要求80所述的方法,其中所述第三区段与所述第一区段具有至少90%互补性。
83.如权利要求80所述的方法,其中所述第三区段与所述第一区段具有至少95%互补性。
84.如权利要求80所述的方法,其中所述第三区段与所述第一区段具有至少98%互补性。
85.如权利要求80所述的方法,其中所述第一区段与鞘翅目昆虫物种互补;并且其中所述第三区段与不同的鞘翅目昆虫物种互补。
86.如权利要求80所述的方法,其中所述第一区段与半翅目昆虫物种互补;并且其中所述第三区段与不同的半翅目昆虫物种互补。
87.如权利要求80所述的方法,其中所述第一区段与鳞翅目昆虫物种互补;并且其中所述第三区段与不同的鳞翅目昆虫物种互补。
88.如权利要求74所述的方法,其中所述植物是单子叶植物。
89.如权利要求88所述的方法,其中所述单子叶植物是玉米、大麦、粟、小麦或稻。
90.如权利要求74所述的方法,其中所述植物是双子叶植物。
91.如权利要求90所述的方法,其中所述双子叶植物是羽衣甘蓝、花椰菜、西兰花、芥菜植物、卷心菜、豌豆、三叶草、苜蓿、蚕豆、番茄、木薯、大豆、卡诺拉油菜、苜蓿、向日葵、红花、烟草、拟南芥、或棉花。
92.一种试剂盒,其包含如权利要求1-28中任一项所述的沉默元件,如权利要求29所述的DNA构建体,或如权利要求30所述的表达构建体,以及使用所述沉默元件、所述DNA构建体、或所述表达构建体作为针对植物有害生物的昆虫不育化剂的说明书。
93.如权利要求92所述的试剂盒,其包含两种或更多种如权利要求1-28中任一项所述的沉默元件。
94.如权利要求92或93所述的试剂盒,其中所述说明书提供了一种或多种沉默元件的顺序施用,以减少所述昆虫有害生物生物体对所述一种或多种沉默元件产生抗性的发生率。
95.如权利要求92或93所述的试剂盒,其中所述说明书提供了一种或多种沉默元件的同时施用,以减少所述昆虫有害生物生物体对所述一种或多种沉默元件产生抗性的发生率。
96.如权利要求92所述的试剂盒,其中所述昆虫植物有害生物为鞘翅目植物有害生物。
97.如权利要求96所述的试剂盒,其中所述鞘翅目植物有害生物为叶甲属植物有害生物。
98.如权利要求97所述的试剂盒,其中所述叶甲属昆虫有害生物生物体包含玉米根萤叶甲、墨西哥玉米根虫、南美叶甲、北方玉米根虫、墨西哥玉米根虫、或斑点黄瓜甲虫。
99.如权利要求96所述的试剂盒,其中所述鞘翅目植物有害生物为条跳甲属植物有害生物。
100.如权利要求99所述的试剂盒,其中条跳甲属植物有害生物为黄曲条跳甲。
101.如权利要求96所述的试剂盒,其中所述鞘翅目植物有害生物为瘦跗叶甲属植物有害生物。
102.如权利要求101所述的试剂盒,其中瘦跗叶甲属植物有害生物为马铃薯甲虫。
103.如权利要求92所述的试剂盒,其中所述昆虫植物有害生物为半翅目植物有害生物。
104.如权利要求103所述的试剂盒,其中所述半翅目植物有害生物为无网蚜属、小粉虱属、褐飞虱属或茶翅蝽属植物有害生物。
105.如权利要求104所述的试剂盒,其中所述无网蚜属植物有害生物为豌豆长管蚜。
106.如权利要求104所述的试剂盒,其中所述小粉虱属植物有害生物为烟粉虱。
107.如权利要求104所述的试剂盒,其中所述褐飞虱属植物有害生物为稻褐飞虱。
108.如权利要求104所述的试剂盒,其中所述茶翅蝽属植物有害生物为茶翅蝽。
109.如权利要求92所述的试剂盒,其中所述昆虫植物有害生物为鳞翅目植物有害生物。
110.如权利要求108所述的试剂盒,其中鳞翅目植物有害生物为斜纹夜蛾。
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EA201890066A1 (ru) | 2018-06-29 |
BR112017027382A2 (pt) | 2018-08-28 |
MX2017016119A (es) | 2018-03-07 |
US10647995B2 (en) | 2020-05-12 |
AU2016278142A1 (en) | 2017-11-30 |
PH12017502320A1 (en) | 2018-06-11 |
US20200231985A1 (en) | 2020-07-23 |
US20220267792A1 (en) | 2022-08-25 |
CA2986265A1 (en) | 2016-12-22 |
EP3310803A1 (en) | 2018-04-25 |
US20180155739A1 (en) | 2018-06-07 |
WO2016205445A1 (en) | 2016-12-22 |
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