DK2069529T3 - Fremgangsmåde til generering af aptamerer med forbedrede dissociationsrater - Google Patents

Fremgangsmåde til generering af aptamerer med forbedrede dissociationsrater

Info

Publication number
DK2069529T3
DK2069529T3 DK08782010.6T DK08782010T DK2069529T3 DK 2069529 T3 DK2069529 T3 DK 2069529T3 DK 08782010 T DK08782010 T DK 08782010T DK 2069529 T3 DK2069529 T3 DK 2069529T3
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Denmark
Prior art keywords
dissociation rates
generating aptamers
improved dissociation
improved
aptamers
Prior art date
Application number
DK08782010.6T
Other languages
Danish (da)
English (en)
Inventor
Dominic Zichi
Sheri K Wilcox
Chris Bock
Daniel J Schneider
Bruce Eaton
Larry Gold
Original Assignee
Somalogic Inc
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Filing date
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40260081&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2069529(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Somalogic Inc filed Critical Somalogic Inc
Application granted granted Critical
Publication of DK2069529T3 publication Critical patent/DK2069529T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C12N2310/3341—5-Methylcytosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2541/00—Reactions characterised by directed evolution
    • C12Q2541/10—Reactions characterised by directed evolution the purpose being the selection or design of target specific nucleic acid binding sequences
    • C12Q2541/101—Selex

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK08782010.6T 2007-07-17 2008-07-17 Fremgangsmåde til generering af aptamerer med forbedrede dissociationsrater DK2069529T3 (da)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US95028307P 2007-07-17 2007-07-17
US95028107P 2007-07-17 2007-07-17
US95029307P 2007-07-17 2007-07-17
US3142008P 2008-02-26 2008-02-26
US5159408P 2008-05-08 2008-05-08
PCT/US2008/070383 WO2009012418A2 (en) 2007-07-17 2008-07-17 Method for generating aptamers with improved off-rates

Publications (1)

Publication Number Publication Date
DK2069529T3 true DK2069529T3 (da) 2013-04-15

Family

ID=40260081

Family Applications (4)

Application Number Title Priority Date Filing Date
DK08782010.6T DK2069529T3 (da) 2007-07-17 2008-07-17 Fremgangsmåde til generering af aptamerer med forbedrede dissociationsrater
DK09012809.1T DK2172566T4 (da) 2007-07-17 2008-07-17 Fremgangsmåde til generering af aptamerer med forbedrede off-rates
DK17181688.7T DK3284832T5 (da) 2007-07-17 2008-07-17 Aptamer med forbedret dissociationshastighed og ikke-kovalent aptamermålkompleks deraf
DK12160299T DK2489743T3 (en) 2007-07-17 2008-07-17 Aptamers with 5- (N-naphthyl) substituted uridines

Family Applications After (3)

Application Number Title Priority Date Filing Date
DK09012809.1T DK2172566T4 (da) 2007-07-17 2008-07-17 Fremgangsmåde til generering af aptamerer med forbedrede off-rates
DK17181688.7T DK3284832T5 (da) 2007-07-17 2008-07-17 Aptamer med forbedret dissociationshastighed og ikke-kovalent aptamermålkompleks deraf
DK12160299T DK2489743T3 (en) 2007-07-17 2008-07-17 Aptamers with 5- (N-naphthyl) substituted uridines

Country Status (17)

Country Link
US (1) US8409795B2 (enExample)
EP (10) EP2933340B1 (enExample)
JP (11) JP5684568B2 (enExample)
KR (3) KR101634608B1 (enExample)
CN (6) CN107090457A (enExample)
AU (3) AU2008275915B2 (enExample)
CA (4) CA2696431C (enExample)
DK (4) DK2069529T3 (enExample)
ES (6) ES2647587T3 (enExample)
FI (1) FI3284832T3 (enExample)
HR (1) HRP20150382T1 (enExample)
MX (7) MX344253B (enExample)
NO (1) NO2933340T3 (enExample)
PL (1) PL2489743T3 (enExample)
PT (2) PT2172566E (enExample)
SI (1) SI2489743T1 (enExample)
WO (3) WO2009012420A1 (enExample)

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KR101634608B1 (ko) 2016-06-29
EP2489743A2 (en) 2012-08-22
JP5956404B2 (ja) 2016-07-27
MX344253B (es) 2016-12-09
EP2172566B1 (en) 2015-03-04
EP2172566A1 (en) 2010-04-07
EP2069496A1 (en) 2009-06-17
EP3284832A2 (en) 2018-02-21
KR20100058485A (ko) 2010-06-03
CN103627792A (zh) 2014-03-12
EP2076613A1 (en) 2009-07-08
JP2019039929A (ja) 2019-03-14
EP2489743A3 (en) 2012-11-21
FI3284832T3 (fi) 2023-08-01
ES2533711T3 (es) 2015-04-14
DK3284832T5 (da) 2024-10-07
CA2696431A1 (en) 2009-01-22
MX2010000533A (es) 2010-04-30
CN101802225A (zh) 2010-08-11

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