CN109843052A - 用于细胞冷冻保存的组合物和方法 - Google Patents

用于细胞冷冻保存的组合物和方法 Download PDF

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Publication number
CN109843052A
CN109843052A CN201780061611.9A CN201780061611A CN109843052A CN 109843052 A CN109843052 A CN 109843052A CN 201780061611 A CN201780061611 A CN 201780061611A CN 109843052 A CN109843052 A CN 109843052A
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cell
composition
cells
freezen protective
cryopreservation composition
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CN201780061611.9A
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Chinese (zh)
Inventor
施冰如
江昆琦
林政毅
黃廷宇
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Transwell Biotech Co ltd
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Transwell Biotech Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/128—Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gels; Chemically altering living parts, e.g. by cross-linking
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/16—Physical preservation processes
    • A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Oncology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
CN201780061611.9A 2016-10-04 2017-10-04 用于细胞冷冻保存的组合物和方法 Pending CN109843052A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662404170P 2016-10-04 2016-10-04
US62/404,170 2016-10-04
US201662405447P 2016-10-07 2016-10-07
US62/405,447 2016-10-07
PCT/CN2017/105253 WO2018064976A1 (en) 2016-10-04 2017-10-04 Compositions and methods for cell cryopreservation

Publications (1)

Publication Number Publication Date
CN109843052A true CN109843052A (zh) 2019-06-04

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Family Applications (2)

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CN201780061611.9A Pending CN109843052A (zh) 2016-10-04 2017-10-04 用于细胞冷冻保存的组合物和方法
CN201780061605.3A Pending CN109790514A (zh) 2016-10-04 2017-10-04 维持细胞存活率的组合物和方法

Family Applications After (1)

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CN201780061605.3A Pending CN109790514A (zh) 2016-10-04 2017-10-04 维持细胞存活率的组合物和方法

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US (2) US10815456B2 (https=)
EP (2) EP3523419B1 (https=)
JP (2) JP7157050B2 (https=)
KR (2) KR102502294B1 (https=)
CN (2) CN109843052A (https=)
CA (2) CA3036391A1 (https=)
TW (2) TWI670371B (https=)
WO (2) WO2018064975A1 (https=)

Cited By (16)

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CN111066776A (zh) * 2019-11-09 2020-04-28 无锡市人民医院 一种卵子冷冻保护剂及其应用
CN111979172A (zh) * 2020-08-31 2020-11-24 安徽惠恩生物科技股份有限公司 一种让细胞保持较长时间活性的输注液及其制备方法
CN112167243A (zh) * 2020-10-14 2021-01-05 中国科学技术大学 红细胞冻存液及快速冻存方法
CN112438252A (zh) * 2020-11-20 2021-03-05 上海欧易生物医学科技有限公司 一种适用于小鼠心脏组织单细胞测序的组织冷冻及解冻方法
CN112616827A (zh) * 2020-11-30 2021-04-09 中国科学技术大学 红细胞冻存方法及冻存的红细胞
CN112649268A (zh) * 2020-12-14 2021-04-13 四川农业大学 精子质膜保护剂hepes/bsa及染色液和染色方法
CN114073249A (zh) * 2021-10-29 2022-02-22 上海原天生物科技有限公司 一种人t淋巴细胞的慢速冻存方法
CN114158549A (zh) * 2020-09-10 2022-03-11 上海原天生物科技有限公司 一种免疫细胞的置核冻存方法
CN114375944A (zh) * 2021-12-31 2022-04-22 四川大学华西医院 一种用于骨组织和骨软组织的冻存液和冻存方法
CN114642200A (zh) * 2022-03-01 2022-06-21 长春科技学院 一种鹿茸生茸区骨膜的冷冻保存方法
CN114667999A (zh) * 2022-05-27 2022-06-28 广东先康达生物科技有限公司 一种免疫细胞冻存稳定剂、冻存液及冻存方法
WO2022134434A1 (zh) * 2020-12-25 2022-06-30 广州赛莱拉干细胞科技股份有限公司 一种外泌体冻存保护液及其制备方法
CN116234441A (zh) * 2020-08-18 2023-06-06 伊奇洛夫科技有限公司 用于器官保存的装置和方法
CN116649328A (zh) * 2023-05-10 2023-08-29 杭州中赢生物医疗科技有限公司 一种人类活性免疫细胞冻存液及其冻存方法
WO2023185487A1 (zh) * 2022-03-29 2023-10-05 中国科学技术大学 卵泡的冷冻保存和复苏方法
CN119699307A (zh) * 2024-12-05 2025-03-28 中国农业科学院北京畜牧兽医研究所 蛋白质去乙酰化酶抑制剂nam在家禽精液超低温冷冻保存中的应用

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US10815456B2 (en) * 2016-10-04 2020-10-27 Transwell Biotech Co., Ltd. Composition, kit and method for cryopreserving cells
US20220125042A1 (en) * 2017-05-17 2022-04-28 Universidade Nova De Lisboa Cryoprotectant and/or cryopreservant composition, methods and uses thereof
KR102503086B1 (ko) * 2017-09-08 2023-02-23 가부시키가이샤 오츠카 세이야쿠 고죠 어린 돼지 유래 줄기 세포 및 그 제조 방법
JP7000212B2 (ja) * 2018-03-14 2022-01-19 旭化成株式会社 幹細胞の凍結保存液
CN108552158A (zh) * 2018-04-09 2018-09-21 广东省中医院（广州中医药大学第二附属医院、广州中医药大学第二临床医学院、广东省中医药科学院） 一种贴壁细胞冻存液、冻存方法和解冻方法
CN108728408B (zh) * 2018-05-28 2021-08-24 天津博雅秀岩生物技术有限公司 犬胎膜间充质干细胞及制备方法和使用的培养基
DK3829604T3 (da) * 2018-07-27 2025-05-19 Cellphire Inc Kryokonserverede blodpladesammensætninger og fremgangsmåder til fremstilling
EP3887404A4 (en) 2018-11-30 2022-11-02 Cellphire, Inc. SLIDES LOADED WITH ANTI-CANCER AGENTS
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TWI837281B (zh) * 2019-01-17 2024-04-01 日商長瀨產業股份有限公司 細胞冷凍保存液及細胞的漸凍方法
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CN109792985A (zh) * 2019-02-20 2019-05-24 华东理工大学 细胞冻存液
WO2020207151A1 (zh) * 2019-04-09 2020-10-15 北京大学第三医院（北京大学第三临床医学院） 一种无dmso的冷冻保存液及其制备方法与应用
JP6830294B1 (ja) * 2019-04-26 2021-02-17 株式会社大塚製薬工場 トレハロースを含む哺乳動物細胞保存用液
CN114007416A (zh) * 2019-04-30 2022-02-01 明尼苏达大学董事会 用于细胞的冷冻保存的组合物和方法
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CN114729317A (zh) 2019-11-29 2022-07-08 新泽西鲁特格斯州立大学 脐带血血浆制品、组织和细胞外泌体的分离方法，其组合物及使用方法
CN114786481A (zh) * 2019-11-29 2022-07-22 新泽西鲁特格斯州立大学 用于储存血液制品的组合物、试剂盒和方法及其使用方法
CN110934132A (zh) * 2019-12-30 2020-03-31 苏州君欣生物科技有限公司 一种无血清无dmso细胞冻存液及其制备方法
KR102542183B1 (ko) * 2019-12-31 2023-06-14 (주)두다 치아 유래 줄기세포의 분리를 위한 치아의 보존 방법
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US12453805B2 (en) 2020-09-24 2025-10-28 Everest Medical Innovation GmbH Cryoprotective compositions, surgical kits, and methods for protection of a surgical site during cryosurgery
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CN116472283A (zh) * 2020-10-29 2023-07-21 国立大学法人大阪大学 细胞结构体的冷冻方法
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JP7266892B2 (ja) * 2020-11-16 2023-05-01 株式会社アビー 細胞凍結保存液、凍結細胞保存方法、及び細胞凍結方法
WO2022158097A1 (ja) * 2021-01-19 2022-07-28 新田ゼラチン株式会社 ウイルス安定化剤、ウイルス安定化剤用ゼラチン加水分解物およびウイルス含有組成物
TW202245814A (zh) 2021-02-17 2022-12-01 美商賽菲爾公司 用於治療抗血小板誘導的凝血病之凍乾血小板衍生物組成物
CN117177663A (zh) * 2021-04-05 2023-12-05 库瑞欧库瑞特有限公司 消除了对细胞渗透性防冻剂的需要的高效生物相容性冷冻保存培养基
IL315637A (en) * 2022-03-22 2024-11-01 Univ Sorbonne A method for preserving cells
JP7818743B2 (ja) * 2022-12-28 2026-02-20 ルースロ ビーヴイ コラーゲン加水分解物を含む凍結保存調製物
EP4662469A1 (en) * 2023-02-08 2025-12-17 Allen Institute Methods of preparing and freezing biological samples for analytical use and associated compositions
WO2025019400A1 (en) * 2023-07-14 2025-01-23 Cryocrate Llc Efficient cryopreservation medium that preserves tissue ultra-structures and eliminates tissue cells
CN117481109B (zh) * 2023-12-26 2024-03-12 北京青藤谷禧干细胞科技研究院有限公司 一种细胞储存介质及储存方法
WO2025183063A1 (ja) * 2024-03-01 2025-09-04 セントラル硝子株式会社 三次元細胞培養体の凍結物、三次元細胞培養体の凍結物を含む容器、細胞凍結キット、凍結保存液、三次元細胞培養体の凍結物の製造方法、三次元細胞培養体の凍結物の使用方法、および凍結保存液の使用方法

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