CA1338207C - Method of assaying of nucleic acids, a reagent combination and kit therefore - Google Patents
Method of assaying of nucleic acids, a reagent combination and kit thereforeInfo
- Publication number
- CA1338207C CA1338207C CA000561135A CA561135A CA1338207C CA 1338207 C CA1338207 C CA 1338207C CA 000561135 A CA000561135 A CA 000561135A CA 561135 A CA561135 A CA 561135A CA 1338207 C CA1338207 C CA 1338207C
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- target nucleic
- primers
- moiety
- capturing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 84
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 84
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 84
- 238000000034 method Methods 0.000 title claims abstract description 63
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 18
- 239000000523 sample Substances 0.000 claims abstract description 111
- 238000009396 hybridization Methods 0.000 claims abstract description 44
- 238000006243 chemical reaction Methods 0.000 claims abstract description 26
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 238000006116 polymerization reaction Methods 0.000 claims description 15
- 230000001419 dependent effect Effects 0.000 claims description 10
- 239000001226 triphosphate Substances 0.000 claims description 5
- 235000011178 triphosphate Nutrition 0.000 claims description 5
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims description 4
- -1 nucleoside triphosphates Chemical class 0.000 claims description 3
- 239000012985 polymerization agent Substances 0.000 claims description 3
- 239000002777 nucleoside Substances 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 27
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 24
- 108020004414 DNA Proteins 0.000 description 23
- 239000000243 solution Substances 0.000 description 18
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 12
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- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2460487A | 1987-03-11 | 1987-03-11 | |
| US024,604 | 1987-03-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1338207C true CA1338207C (en) | 1996-04-02 |
Family
ID=21821443
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000561135A Expired - Fee Related CA1338207C (en) | 1987-03-11 | 1988-03-10 | Method of assaying of nucleic acids, a reagent combination and kit therefore |
Country Status (25)
| Country | Link |
|---|---|
| US (2) | US5476769A (OSRAM) |
| JP (1) | JP3244500B2 (OSRAM) |
| AT (1) | AT395434B (OSRAM) |
| AU (1) | AU622104B2 (OSRAM) |
| BE (1) | BE1003990A5 (OSRAM) |
| CA (1) | CA1338207C (OSRAM) |
| CH (1) | CH677285A5 (OSRAM) |
| DD (1) | DD272664A5 (OSRAM) |
| DE (1) | DE3807994C2 (OSRAM) |
| DK (1) | DK175384B1 (OSRAM) |
| ES (1) | ES2006377A6 (OSRAM) |
| FI (1) | FI94429C (OSRAM) |
| FR (1) | FR2613077A1 (OSRAM) |
| GB (1) | GB2202328B (OSRAM) |
| GR (1) | GR1000315B (OSRAM) |
| HU (1) | HU202583B (OSRAM) |
| IE (1) | IE61664B1 (OSRAM) |
| IL (3) | IL85480A0 (OSRAM) |
| IT (1) | IT1216048B (OSRAM) |
| LU (1) | LU87153A1 (OSRAM) |
| NL (1) | NL194922C (OSRAM) |
| NO (1) | NO172909C (OSRAM) |
| NZ (1) | NZ223700A (OSRAM) |
| PT (1) | PT86937B (OSRAM) |
| SE (1) | SE500262C2 (OSRAM) |
Families Citing this family (177)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5753433A (en) * | 1909-12-05 | 1998-05-19 | Boehringer Mannheim Gmbh | Method for the sensitive detection of nucleic acids |
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| FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
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1988
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- 1988-03-10 BE BE8800267A patent/BE1003990A5/fr not_active IP Right Cessation
- 1988-03-10 GB GB8805681A patent/GB2202328B/en not_active Expired - Lifetime
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- 1988-03-10 IE IE69788A patent/IE61664B1/en not_active IP Right Cessation
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- 1988-03-10 DE DE3807994A patent/DE3807994C2/de not_active Expired - Lifetime
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- 1988-03-10 FR FR8803107A patent/FR2613077A1/fr active Granted
- 1988-03-10 PT PT86937A patent/PT86937B/pt not_active IP Right Cessation
- 1988-03-11 ES ES8800740A patent/ES2006377A6/es not_active Expired
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1990
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1992
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