JP3244500B2 - 核酸のアッセイ法ならびにそれに用いる試薬およびキット - Google Patents
核酸のアッセイ法ならびにそれに用いる試薬およびキットInfo
- Publication number
- JP3244500B2 JP3244500B2 JP05731088A JP5731088A JP3244500B2 JP 3244500 B2 JP3244500 B2 JP 3244500B2 JP 05731088 A JP05731088 A JP 05731088A JP 5731088 A JP5731088 A JP 5731088A JP 3244500 B2 JP3244500 B2 JP 3244500B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- primer
- copy
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 20
- 238000007826 nucleic acid assay Methods 0.000 title claims description 8
- 239000000523 sample Substances 0.000 claims description 148
- 150000007523 nucleic acids Chemical class 0.000 claims description 121
- 108020004707 nucleic acids Proteins 0.000 claims description 120
- 102000039446 nucleic acids Human genes 0.000 claims description 120
- 238000001514 detection method Methods 0.000 claims description 107
- 238000000034 method Methods 0.000 claims description 65
- 238000009396 hybridization Methods 0.000 claims description 57
- 238000003556 assay Methods 0.000 claims description 37
- 238000006243 chemical reaction Methods 0.000 claims description 27
- 238000006116 polymerization reaction Methods 0.000 claims description 22
- 230000001419 dependent effect Effects 0.000 claims description 11
- 239000001226 triphosphate Substances 0.000 claims description 5
- 235000011178 triphosphate Nutrition 0.000 claims description 5
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims description 5
- 238000002372 labelling Methods 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims 2
- 244000068988 Glycine max Species 0.000 claims 2
- 239000012985 polymerization agent Substances 0.000 claims 2
- 239000011541 reaction mixture Substances 0.000 description 22
- 239000000243 solution Substances 0.000 description 20
- 108020004414 DNA Proteins 0.000 description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
- 229910052774 Proactinium Inorganic materials 0.000 description 14
- 229910052745 lead Inorganic materials 0.000 description 13
- 239000013612 plasmid Substances 0.000 description 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
- 230000006820 DNA synthesis Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 239000011780 sodium chloride Substances 0.000 description 9
- 241000701022 Cytomegalovirus Species 0.000 description 8
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 6
- 210000002700 urine Anatomy 0.000 description 6
- 102100034343 Integrase Human genes 0.000 description 5
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 5
- 229960002685 biotin Drugs 0.000 description 5
- 235000020958 biotin Nutrition 0.000 description 5
- 239000011616 biotin Substances 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 108091033319 polynucleotide Proteins 0.000 description 5
- 102000040430 polynucleotide Human genes 0.000 description 5
- 239000002157 polynucleotide Substances 0.000 description 5
- 239000001488 sodium phosphate Substances 0.000 description 5
- 229910000162 sodium phosphate Inorganic materials 0.000 description 5
- 108010051423 streptavidin-agarose Proteins 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 5
- 229920000936 Agarose Polymers 0.000 description 4
- 102000004594 DNA Polymerase I Human genes 0.000 description 4
- 108010017826 DNA Polymerase I Proteins 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000001509 sodium citrate Substances 0.000 description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000012876 carrier material Substances 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000005497 microtitration Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000001668 nucleic acid synthesis Methods 0.000 description 3
- 102000013415 peroxidase activity proteins Human genes 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000000644 propagated effect Effects 0.000 description 3
- 239000000376 reactant Substances 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102000008857 Ferritin Human genes 0.000 description 2
- 108050000784 Ferritin Proteins 0.000 description 2
- 238000008416 Ferritin Methods 0.000 description 2
- 229920001917 Ficoll Polymers 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000010523 cascade reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- -1 heme compounds Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- CZIHNRWJTSTCEX-UHFFFAOYSA-N 2 Acetylaminofluorene Chemical compound C1=CC=C2C3=CC=C(NC(=O)C)C=C3CC2=C1 CZIHNRWJTSTCEX-UHFFFAOYSA-N 0.000 description 1
- SNBCLPGEMZEWLU-QXFUBDJGSA-N 2-chloro-n-[[(2r,3s,5r)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methyl]acetamide Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CNC(=O)CCl)[C@@H](O)C1 SNBCLPGEMZEWLU-QXFUBDJGSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 206010011831 Cytomegalovirus infection Diseases 0.000 description 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000975394 Evechinus chloroticus Species 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 102100026933 Myelin-associated neurite-outgrowth inhibitor Human genes 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- JGQHTPYIIXRFLR-UHFFFAOYSA-N [2-(9h-fluoren-1-yl)-2-oxoethyl] acetate Chemical class C1C2=CC=CC=C2C2=C1C(C(=O)COC(=O)C)=CC=C2 JGQHTPYIIXRFLR-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229940094991 herring sperm dna Drugs 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229910052747 lanthanoid Inorganic materials 0.000 description 1
- 150000002602 lanthanoids Chemical class 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000006277 sulfonation reaction Methods 0.000 description 1
- 125000000542 sulfonic acid group Chemical group 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2460487A | 1987-03-11 | 1987-03-11 | |
| US024604 | 1987-03-11 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63243875A JPS63243875A (ja) | 1988-10-11 |
| JP3244500B2 true JP3244500B2 (ja) | 2002-01-07 |
Family
ID=21821443
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP05731088A Expired - Lifetime JP3244500B2 (ja) | 1987-03-11 | 1988-03-10 | 核酸のアッセイ法ならびにそれに用いる試薬およびキット |
Country Status (25)
| Country | Link |
|---|---|
| US (2) | US5476769A (OSRAM) |
| JP (1) | JP3244500B2 (OSRAM) |
| AT (1) | AT395434B (OSRAM) |
| AU (1) | AU622104B2 (OSRAM) |
| BE (1) | BE1003990A5 (OSRAM) |
| CA (1) | CA1338207C (OSRAM) |
| CH (1) | CH677285A5 (OSRAM) |
| DD (1) | DD272664A5 (OSRAM) |
| DE (1) | DE3807994C2 (OSRAM) |
| DK (1) | DK175384B1 (OSRAM) |
| ES (1) | ES2006377A6 (OSRAM) |
| FI (1) | FI94429C (OSRAM) |
| FR (1) | FR2613077A1 (OSRAM) |
| GB (1) | GB2202328B (OSRAM) |
| GR (1) | GR1000315B (OSRAM) |
| HU (1) | HU202583B (OSRAM) |
| IE (1) | IE61664B1 (OSRAM) |
| IL (3) | IL85480A0 (OSRAM) |
| IT (1) | IT1216048B (OSRAM) |
| LU (1) | LU87153A1 (OSRAM) |
| NL (1) | NL194922C (OSRAM) |
| NO (1) | NO172909C (OSRAM) |
| NZ (1) | NZ223700A (OSRAM) |
| PT (1) | PT86937B (OSRAM) |
| SE (1) | SE500262C2 (OSRAM) |
Families Citing this family (175)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4038804A1 (de) | 1990-10-09 | 1992-04-16 | Boehringer Mannheim Gmbh | Verfahren zur genus- oder/und spezies-spezifischen detektion von bakterien in einer probenfluessigkeit |
| US5753433A (en) * | 1909-12-05 | 1998-05-19 | Boehringer Mannheim Gmbh | Method for the sensitive detection of nucleic acids |
| US5714380A (en) | 1986-10-23 | 1998-02-03 | Amoco Corporation | Closed vessel for isolating target molecules and for performing amplification |
| FI80476C (fi) * | 1987-10-09 | 1990-06-11 | Orion Yhtymae Oy | Foerbaettrat hybridisationsfoerfarande, vid foerfarandet anvaent redskap och reagensfoerpackning. |
| JPH0775560B2 (ja) * | 1987-12-25 | 1995-08-16 | 湧永製薬株式会社 | 検体中の目的核酸の検出法 |
| CA1325761C (en) * | 1987-12-25 | 1994-01-04 | Takanori Oka | Method of detecting an intended nucleic acid in a sample |
| WO1989009281A1 (en) * | 1988-03-25 | 1989-10-05 | Akzo N.V. | Method for amplifying and detecting nucleic acid in a test liquid |
| US5817797A (en) * | 1988-06-01 | 1998-10-06 | The United States Of America As Represented By The Department Of Health And Human Services | Sequencing DNA; a modification of the polymerase chain reaction |
| US5858652A (en) * | 1988-08-30 | 1999-01-12 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| AU629845B2 (en) * | 1988-08-30 | 1992-10-15 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| CA2002076A1 (en) * | 1988-11-21 | 1990-05-21 | Brent A. Burdick | Diagnostic kit and method using a solid phase capture means for detecting nucleic acids |
| GB8827160D0 (en) * | 1988-11-21 | 1988-12-29 | Apothekernes Lab | Detection & quantitative determination of rna & dna |
| IL92474A (en) * | 1988-11-29 | 1994-04-12 | Orion Yhtymae Oy | Reaction method and combination for determining nucleotide sequences |
| US5536648A (en) * | 1988-12-09 | 1996-07-16 | Amrad Corporation Limited | Amplified DNA assay using a double stranded DNA binding protein |
| WO1990006374A1 (en) * | 1988-12-09 | 1990-06-14 | Amrad Corporation Limited | Amplified dna assay |
| CA2004326A1 (en) * | 1988-12-23 | 1990-06-23 | Nanibushan Dattagupta | Assay of sequences using amplified genes |
| ES2093633T3 (es) * | 1989-01-19 | 1997-01-01 | Behringwerke Ag | Amplificacion de acidos nucleicos utilizando un unico cebador. |
| DE69033179T3 (de) * | 1989-02-13 | 2005-01-27 | Geneco Pty. Ltd. | Nachweis einer nukleinsäuresequenz oder einer änderung darin |
| US5856092A (en) * | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
| CA2011818A1 (en) * | 1989-03-17 | 1990-09-17 | Fred T. Oakes | Methods for purification, amplification and detection of a nucleic acid |
| CA2013317A1 (en) * | 1989-04-17 | 1990-10-17 | Fred T. Oakes | Diagnostic kit, primer composition and their use for replication or detection of nucleic acids |
| WO1990015881A1 (fr) * | 1989-06-12 | 1990-12-27 | Cis Bio International | Procede de detection de sequences specifiques d'acides nucleiques et ses applications |
| GB8920097D0 (en) * | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
| US5196305A (en) * | 1989-09-12 | 1993-03-23 | Eastman Kodak Company | Diagnostic and amplification methods using primers having thymine at 3' end to overcome primer-target mismatch at the 3' end |
| US5232829A (en) * | 1989-09-29 | 1993-08-03 | Hoffmann-La Roche Inc. | Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes |
| IL97222A (en) * | 1990-02-16 | 1995-08-31 | Orion Yhtymae Oy | Method and reagent for determining specific nucleotide variations |
| US6013431A (en) | 1990-02-16 | 2000-01-11 | Molecular Tool, Inc. | Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators |
| US5387505A (en) * | 1990-05-04 | 1995-02-07 | Eastman Kodak Company | Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage |
| NZ240079A (en) * | 1990-10-09 | 1993-07-27 | Boehringer Mannheim Gmbh | Method for the detection of a nucleic acid or part thereof |
| EP0566670A4 (en) * | 1990-12-17 | 1993-12-08 | Idexx Laboratories, Inc. | Nucleic acid sequence detection by triple helix formation |
| DE4106251A1 (de) * | 1991-02-28 | 1992-09-03 | Boehringer Mannheim Gmbh | Nachweis von bakterien mit hilfe einer nukleinsaeureamplifikation |
| US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
| US5888819A (en) * | 1991-03-05 | 1999-03-30 | Molecular Tool, Inc. | Method for determining nucleotide identity through primer extension |
| CA2065719A1 (en) * | 1991-04-30 | 1992-10-31 | John B. Findlay | Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle |
| US5387510A (en) * | 1991-10-02 | 1995-02-07 | Eastman Kodak Company | Detection of amplified nucleic acid using secondary capture oligonucleotides and test kit |
| IT1252295B (it) * | 1991-11-15 | 1995-06-08 | Schiapparelli Diagnostici Ismu | Metodo per rivelare acidi nucleici e relativo kit diagnostico |
| CA2124796A1 (en) * | 1991-12-23 | 1993-07-08 | Chiron Diagnostics Corporation | Cmv probes for use in solution phase sandwich hybridization assays |
| US5863724A (en) * | 1994-04-13 | 1999-01-26 | Baylor College Of Medicine | Methods of screening for persistent hyperinsulinemic hypoglycemia of infancy |
| US5658729A (en) * | 1994-10-11 | 1997-08-19 | The University Of British Columbia | Method, reagent and kit for evaluating susceptibility to premature atherosclerosis |
| DE4421901A1 (de) * | 1994-06-23 | 1996-01-04 | Bayer Ag | Ein DNA-Schnelltest zum Nachweis von chinolonresistenten Staphylococcus aureus Erregern in klinischem Probenmaterial |
| US5705366A (en) * | 1994-09-15 | 1998-01-06 | Johnson & Johnson Clinical Diagnostics, Inc. | Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor |
| US5654141A (en) * | 1994-11-18 | 1997-08-05 | Thomas Jefferson University | Amplification based detection of bacterial infection |
| US5856096A (en) * | 1995-09-20 | 1999-01-05 | Ctrc Research Foundation | Rapid and sensitive assays for detecting and distinguishing between processive and non-processive telomerase activities |
| AU2320597A (en) | 1996-03-19 | 1997-10-10 | Molecular Tool, Inc. | Method for determining the nucleotide sequence of a polynucleotide |
| US6132954A (en) * | 1996-08-20 | 2000-10-17 | Baylor College Of Medicine | Methods of screening for agents that delay a cell cycle and compositions comprising era and an analogue of wild-type era |
| US6071493A (en) | 1996-09-20 | 2000-06-06 | Baylor College Of Medicine | Method of screening for an agent that inhibits mononuclear phagocyte-plaque component complex formation |
| US6043283A (en) * | 1996-09-20 | 2000-03-28 | Baylor College Of Medicine | Tyramine compounds and their neuronal effects |
| US6262242B1 (en) | 1997-01-30 | 2001-07-17 | Board Of Regents, The University Of Texas System | Tumor suppressor designated TS10Q23.3 |
| US6482795B1 (en) | 1997-01-30 | 2002-11-19 | Myriad Genetics, Inc. | Tumor suppressor designated TS10q23.3 |
| US6713300B1 (en) | 1997-02-27 | 2004-03-30 | University Of Utah Research Foundation | Nucleic acid and amino acid sequences for ATP-binding cassette transporter and methods of screening for agents that modify ATP-binding cassette transporter |
| IL122147A0 (en) * | 1997-11-10 | 1998-04-05 | Technion Res & Dev Foundation | Method for labeling polynucleotides |
| PT1040192E (pt) | 1997-12-18 | 2006-12-29 | Monsanto Technology Llc | Plantas transgénicas resistentes a insectos e métodos para melhoramento da actividade 8-endotoxina contra insectos |
| US6673914B1 (en) | 1998-01-22 | 2004-01-06 | John Wayne Cancer Institute | Human tumor-associated gene |
| US20020147143A1 (en) | 1998-03-18 | 2002-10-10 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of lung cancer |
| DE19824900B4 (de) | 1998-06-04 | 2006-05-04 | Roche Diagnostics Gmbh | DNA-Nachweis über einen Strangreassoziationskomplex |
| US6743906B1 (en) | 1998-10-02 | 2004-06-01 | Board Of Regents, The University Of Texas | PPP2R1B is a tumor suppressor |
| US6468523B1 (en) | 1998-11-02 | 2002-10-22 | Monsanto Technology Llc | Polypeptide compositions toxic to diabrotic insects, and methods of use |
| CN1230444C (zh) | 1998-11-16 | 2005-12-07 | 德克萨斯州立大学董事会 | 含有hiv细胞毒t淋巴细胞表位的hiv多肽或肽或其多聚核苷酸表达构建体的用途 |
| US6432642B1 (en) | 1999-01-15 | 2002-08-13 | Pe Corporation (Ny) | Binary probe and clamp composition and methods for a target hybridization detection |
| US6156515A (en) * | 1999-02-09 | 2000-12-05 | Urocor, Inc. | Prostate-specific gene for diagnosis, prognosis and management of prostate cancer |
| AU4807400A (en) | 1999-04-30 | 2000-11-17 | University Of Florida | Adeno-associated virus-delivered ribozyme compositions and methods of use |
| JP2003530817A (ja) | 1999-06-01 | 2003-10-21 | ベイラー カレッジ オブ メディスン | 聴覚障害、変形性関節症および細胞の異常増殖に関するａｔｏｎａｌ関連配列の治療用途のための組成物および方法 |
| US20040002068A1 (en) | 2000-03-01 | 2004-01-01 | Corixa Corporation | Compositions and methods for the detection, diagnosis and therapy of hematological malignancies |
| EP1278855B1 (en) | 2000-04-21 | 2008-03-12 | Corixa Corporation | Compounds and methods for treatment and diagnosis of chlamydial infection |
| WO2002000174A2 (en) | 2000-06-28 | 2002-01-03 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of lung cancer |
| CA2415422C (en) | 2000-07-10 | 2014-07-08 | Board Of Regents, The University Of Texas System | Chromosome 3p21.3 genes are tumor suppressors |
| EP1988097A1 (en) | 2001-05-09 | 2008-11-05 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of prostate cancer |
| US7452705B2 (en) | 2001-05-22 | 2008-11-18 | The University Of Chicago | N4 virion single-stranded DNA dependent RNA polymerase |
| EP1908851A3 (en) | 2001-09-19 | 2008-06-25 | Intergenetics Incorporated | Genetic analysis for stratification of cancer risk |
| JP2005532780A (ja) | 2001-09-19 | 2005-11-04 | インタージェネティックス インコーポレイテッド | 発癌リスク層別化のための遺伝的解析 |
| ES2205998B1 (es) * | 2001-12-14 | 2005-08-16 | Consejo Sup. Investig. Cientificas. | Procedimiento para la deteccion del virus del amarilleo de las venas del pepino (cvyv). |
| ES2405790T3 (es) | 2001-12-17 | 2013-06-03 | Corixa Corporation | Composiciones y métodos para la terapia y el diagnóstico de enfermedad inflamatoria del intestino |
| ATE443133T1 (de) | 2002-02-01 | 2009-10-15 | Life Technologies Corp | Oligonukleotidzusammensetzungen mit verbesserter effizienz |
| US20060009409A1 (en) | 2002-02-01 | 2006-01-12 | Woolf Tod M | Double-stranded oligonucleotides |
| JP4570565B2 (ja) | 2002-07-15 | 2010-10-27 | ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム | ペリプラズム発現によるコンビナトリアルタンパク質ライブラリーのスクリーニング |
| KR20120002613A (ko) | 2002-08-12 | 2012-01-06 | 제네렉스, 인코포레이티드 | 폭스바이러스 및 암과 관련된 방법 및 조성물 |
| AU2002951411A0 (en) | 2002-09-16 | 2002-09-26 | The University Of Sydney | Genotype screen |
| PT1589934E (pt) | 2003-01-06 | 2016-01-26 | Corixa Corp | Determinados compostos de fosfato de aminoalquil glucosaminida e seu uso |
| US7960522B2 (en) | 2003-01-06 | 2011-06-14 | Corixa Corporation | Certain aminoalkyl glucosaminide phosphate compounds and their use |
| DK2374900T3 (en) | 2003-03-07 | 2016-10-17 | Rubicon Genomics Inc | Polynucleotides for amplification and analysis of the total genomic and total transcription libraries generated by a DNA polymerization |
| CN1882688B (zh) | 2003-07-25 | 2014-07-16 | 安比恩股份有限公司 | 用于分离小rna分子的方法和组合物 |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| EP1766014A4 (en) | 2004-06-03 | 2008-12-10 | Athlomics Pty Ltd | AGENTS AND METHODS FOR THE DIAGNOSIS OF STRESS |
| WO2006017724A1 (en) * | 2004-08-06 | 2006-02-16 | Becton, Dickinson And Company | Sequences and methods for detection of cytomegalovirus |
| ATE469245T1 (de) | 2004-09-14 | 2010-06-15 | Univ Colorado | Auf genetischem targeting basierendes verfahren zur behandlung mit bucindolol |
| CA2594483C (en) | 2005-01-28 | 2014-08-26 | Duke University | Apparatuses and methods for manipulating droplets on a printed circuit board |
| ES2381279T3 (es) | 2005-05-06 | 2012-05-24 | Gen-Probe Incorporated | Métodos y productos para la captura de ácido nucleico diana |
| NZ564923A (en) | 2005-07-07 | 2012-02-24 | Athlomics Pty Ltd | Polynucleotide marker genes and their expression, for diagnosis of endotoxemia |
| US7494788B2 (en) | 2005-07-11 | 2009-02-24 | Molecular Kinetics, Inc. | Entropic bristle domain sequences and their use in recombinant protein production |
| US8980246B2 (en) | 2005-09-07 | 2015-03-17 | Sillajen Biotherapeutics, Inc. | Oncolytic vaccinia virus cancer therapy |
| CN101370946B (zh) | 2005-10-21 | 2011-07-20 | 基因信息股份有限公司 | 用于使生物标志产物水平与疾病相关联的方法和装置 |
| EP2368570A3 (en) | 2006-01-18 | 2012-05-02 | University Of Chicago | Compositions and methods related to staphylococcal bacterium proteins |
| WO2007106579A2 (en) | 2006-03-15 | 2007-09-20 | Micronics, Inc. | Integrated nucleic acid assays |
| ES2741138T3 (es) | 2006-09-15 | 2020-02-10 | Childrens Hospital Of Eastern Ontario Res Institute Inc | Rhabdovirus oncolítico de Farmington |
| ATE555128T1 (de) | 2006-11-30 | 2012-05-15 | Res Dev Foundation | Verbesserte immunglobulin-bibliotheken |
| US9938641B2 (en) * | 2006-12-18 | 2018-04-10 | Fluidigm Corporation | Selection of aptamers based on geometry |
| HRP20161150T1 (hr) | 2007-04-09 | 2016-11-18 | University Of Florida Research Foundation, Inc. | PRIPRAVCI rAAV VEKTORA KOJI IMAJU TIROZIN-MODIFICIRANE CAPSID PROTEINE I POSTUPCI ZA UPORABU |
| DK2155789T3 (da) | 2007-05-01 | 2013-10-21 | Res Dev Foundation | Immunoglobulin-Fc-biblioteker |
| AU2009302582A1 (en) | 2008-10-06 | 2010-04-15 | University Of Chicago | Compositions and methods related to bacterial Eap, Emp, and/or AdsA proteins |
| SI3281947T1 (sl) | 2009-04-03 | 2020-07-31 | The University Of Chicago | Sestave in metode v zvezi z variantami proteina A (SPA) |
| CN102458446B (zh) | 2009-04-22 | 2017-06-13 | 印第安那大学科技研究公司 | 用于治疗慢性阻塞性肺病和哮喘的组合物和方法 |
| EP2789689B1 (en) | 2009-06-29 | 2016-04-27 | Luminex Corporation | Chimeric primers with hairpin conformations and methods of using same |
| EP2272979A1 (en) | 2009-06-30 | 2011-01-12 | Centre National de la Recherche Scientifique (CNRS) | Method for testing a subject thought to be predisposed to having cancer |
| DK3042957T3 (en) | 2009-07-17 | 2018-01-02 | Bioatla Llc | At the same time, integrated selection and development of human protein performance and expression in production hosts |
| US9409983B2 (en) | 2009-07-23 | 2016-08-09 | The Board Of Trustess Of The University Of Illinois | Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases |
| US9512481B2 (en) | 2009-09-11 | 2016-12-06 | The Regents Of The University Of Colorado, A Body Corporate | Polymorphisms in the PDE3A gene |
| ES2671570T3 (es) | 2009-09-14 | 2018-06-07 | Sillajen Biotherapeutics, Inc. | Terapia combinada contra el cáncer con virus oncolítico de vaccinia |
| WO2011070440A2 (en) | 2009-12-10 | 2011-06-16 | Ottawa Hospital Research Institute | Oncolytic rhabdovirus |
| EP2515899B1 (en) | 2009-12-23 | 2016-05-25 | ARCA biopharma, Inc. | Methods and compositions for cardiovascular diseases and conditions |
| KR101851117B1 (ko) | 2010-01-29 | 2018-04-23 | 마이크로닉스 인코포레이티드. | 샘플-투-앤서 마이크로유체 카트리지 |
| JP2013523818A (ja) | 2010-04-05 | 2013-06-17 | ザ・ユニバーシティー・オブ・シカゴ | 免疫反応のエンハンサーとしてのプロテインＡ（ＳｐＡ）抗体に関連する組成物および方法 |
| EA035893B1 (ru) | 2010-04-23 | 2020-08-27 | Юниверсити Оф Флорида Рисерч Фаундейшн, Инк. | РЕКОМБИНАНТНЫЙ АДЕНОАССОЦИИРОВАННЫЙ ВИРУСНЫЙ ВЕКТОР (rAAV), КОДИРУЮЩИЙ БИОЛОГИЧЕСКИ АКТИВНУЮ, СПЕЦИФИЧНУЮ В ОТНОШЕНИИ СЕТЧАТКИ ГУАНИЛАТЦИКЛАЗУ ЧЕЛОВЕКА, СПОСОБНЫЙ ПРЕДОХРАНЯТЬ ИЛИ ВОССТАНАВЛИВАТЬ ОПОСРЕДОВАННУЮ КОЛБОЧКАМИ ФУНКЦИЮ ГЛАЗА МЛЕКОПИТАЮЩЕГО |
| CA2803298C (en) | 2010-07-02 | 2020-07-14 | The University Of Chicago | Compositions and methods related to protein a (spa) variants |
| SG187033A1 (en) | 2010-07-16 | 2013-02-28 | Bioatla Llc | Novel methods of protein evolution |
| JP5793194B2 (ja) | 2010-09-09 | 2015-10-14 | ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ | 感染防御ブドウ球菌抗原が関与する方法および組成物 |
| EP2455485A1 (de) | 2010-11-19 | 2012-05-23 | Anagnostics Bioanalysis GmbH | Verfahren zum Nachweis von Nukleinsäuren |
| CN103429258B (zh) | 2011-01-04 | 2016-03-09 | 新罗杰公司 | 通过施用溶瘤痘苗病毒生成针对肿瘤抗原的抗体和生成肿瘤特异性补体依赖性细胞毒性 |
| JP6228014B2 (ja) | 2011-02-07 | 2017-11-08 | リサーチ ディベロップメント ファウンデーション | 操作された免疫グロブリンＦｃポリペプチド |
| MX2013009863A (es) | 2011-02-28 | 2013-12-06 | Univ Iowa Res Found | Cambios de la hormona anti-mulleriana en el embarazo y prediccion de sexo y de resultados adversos en el embarazo. |
| WO2012120377A2 (en) | 2011-03-08 | 2012-09-13 | King Abdullah University Of Science And Technology | Molecular biomarker set for early detection of ovarian cancer |
| EP2694534B1 (en) | 2011-04-08 | 2018-06-20 | Evaxion Biotech ApS | Proteins and nucleic acids useful in vaccines targeting staphylococcus aureus |
| US8945588B2 (en) | 2011-05-06 | 2015-02-03 | The University Of Chicago | Methods and compositions involving protective staphylococcal antigens, such as EBH polypeptides |
| EP2718427B1 (en) | 2011-06-08 | 2017-01-11 | Children's Hospital of Eastern Ontario Research Institute Inc. | Compositions for glioblastoma treatment |
| MX2013013627A (es) | 2011-06-21 | 2014-04-25 | Oncofactor Corp | Composiciones y metodos para la terapia y diagnostico de cancer. |
| WO2013036799A2 (en) | 2011-09-09 | 2013-03-14 | Fred Hutchinson Cancer Research Center | Methods and compositions involving nkg2d inhibitors and cancer |
| WO2013053899A1 (en) | 2011-10-12 | 2013-04-18 | Moeller Niels Iversen | Peptides derived from campylobacter jejuni and their use in vaccination |
| US20140349858A1 (en) | 2011-12-22 | 2014-11-27 | Ibis Bioscience, Inc. | Amplification of a sequence from a ribonucleic acid |
| JP6196241B2 (ja) | 2012-02-02 | 2017-09-13 | インベンラ， インコーポレイテッド | 生物学的に活性なポリペプチドのハイスループットスクリーニング |
| WO2013162746A1 (en) | 2012-04-26 | 2013-10-31 | University Of Chicago | Staphylococcal coagulase antigens and methods of their use |
| JP6248633B2 (ja) * | 2012-08-31 | 2017-12-20 | 東レ株式会社 | 標的核酸の検出方法 |
| ES2730690T3 (es) | 2012-12-07 | 2019-11-12 | Suppremol Gmbh | Estratificación y tratamiento de pacientes que padecen púrpura trombocitopénica idiopática |
| EP2935559B1 (en) | 2012-12-21 | 2020-09-16 | PerkinElmer Health Sciences, Inc. | Fluorescence detection system |
| CN104919191B (zh) | 2012-12-21 | 2019-07-09 | 精密公司 | 流体回路及相关的制造方法 |
| CN104994957B (zh) | 2012-12-21 | 2017-11-28 | 精密公司 | 用于微流体用途的低弹性膜 |
| US10125373B2 (en) | 2013-01-22 | 2018-11-13 | Arizona Board Of Regents On Behalf Of Arizona State University | Geminiviral vector for expression of rituximab |
| EP3622965A1 (en) | 2013-02-21 | 2020-03-18 | Turnstone Limited Partnership | Vaccine composition |
| US10386377B2 (en) | 2013-05-07 | 2019-08-20 | Micronics, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
| WO2014182847A1 (en) | 2013-05-07 | 2014-11-13 | Micronics, Inc. | Device for preparation and analysis of nucleic acids |
| AU2014262710B2 (en) | 2013-05-07 | 2019-09-12 | Revvity Health Sciences, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
| US20150098940A1 (en) | 2013-10-03 | 2015-04-09 | Oklahoma Medical Research Foundation | Biomarkers for Systemic Lupus Erythematosus Disease Activity, and Intensity and Flare |
| ES3012932T3 (en) | 2013-12-03 | 2025-04-10 | Evaxion Biotech As | Proteins and nucleic acids useful in vaccines targeting staphylococcus aureus |
| DK3092491T3 (en) | 2014-01-07 | 2019-02-11 | Bioatla Llc | Proteins that are targeted orthologs |
| EP3154693B1 (en) | 2014-06-11 | 2021-11-03 | PerkinElmer Health Sciences, Inc. | Method for performing a sample assay with a microfluidic cartridges with integrated assay controls |
| EP3218002B1 (en) | 2014-11-13 | 2020-08-19 | Evaxion Biotech ApS | Peptides derived from acinetobacter baumannii and their use in vaccination |
| CN107428821B (zh) | 2014-12-08 | 2022-06-24 | 博格有限责任公司 | 包括细丝蛋白a的标志物在诊断和治疗前列腺癌中的用途 |
| EP3485907B1 (en) | 2015-01-12 | 2023-06-28 | Evaxion Biotech ApS | Treatment and prophylaxis of k. pneumoniae infection |
| WO2016130516A1 (en) | 2015-02-09 | 2016-08-18 | Research Development Foundation | Engineered immunoglobulin fc polypeptides displaying improved complement activation |
| EP3070178B1 (en) | 2015-03-20 | 2020-02-12 | Albert-Ludwigs-Universität Freiburg | Method for diagnosing breast cancer |
| WO2017005670A1 (en) | 2015-07-04 | 2017-01-12 | Evaxion Biotech Aps | Proteins and nucleic acids useful in vaccines targeting pseudomonas aeruginosa |
| RU2018105835A (ru) | 2015-07-17 | 2019-08-19 | Президент Энд Фэллоуз Оф Харвард Коллидж | Способы амплификации последовательностей нуклеиновых кислот |
| US10526408B2 (en) | 2015-08-28 | 2020-01-07 | Research Development Foundation | Engineered antibody FC variants |
| US10946084B2 (en) | 2016-02-22 | 2021-03-16 | Evaxion Biotech Aps | Proteins and nucleic acids useful in vaccines targeting Staphylococcus aureus |
| AU2017278261A1 (en) | 2016-06-05 | 2019-01-31 | Berg Llc | Systems and methods for patient stratification and identification of potential biomarkers |
| US11718648B2 (en) | 2017-01-05 | 2023-08-08 | Evaxion Biotech A/S | Vaccines targeting Pseudomonas aeruginosa |
| AU2018213315A1 (en) | 2017-01-26 | 2019-07-25 | Oklahoma Medical Research Foundation | Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare |
| US12140592B2 (en) | 2017-06-16 | 2024-11-12 | Oklahoma Medical Research Foundation | Biomarkers for assessing risk of transitioning to systemic lupus erythematosus classification and disease pathogenesis |
| KR102554477B1 (ko) | 2018-10-18 | 2023-07-11 | 오클라호마 메디컬 리써치 화운데이션 | 질병 활성도의 특징을 규명하는 전신 홍반성 낭창(sle) 질병 활성도 면역 지수를 위한 생체지표 |
| WO2020083904A1 (en) | 2018-10-22 | 2020-04-30 | Evaxion Biotech Aps | Vaccines targeting m. catharrhalis |
| EP3931206A1 (en) | 2019-02-27 | 2022-01-05 | Evaxion Biotech ApS | Vaccines targeting h. influenzae |
| CA3138566A1 (en) | 2019-04-30 | 2020-11-05 | Larimar Therapeutics, Inc. | Frataxin-sensitive markers for determining effectiveness of frataxin replacement therapy |
| EP3772543B1 (en) | 2019-08-09 | 2023-09-27 | Albert-Ludwigs-Universität Freiburg | Method for diagnosing breast cancer |
| CA3151317A1 (en) | 2019-09-16 | 2021-03-25 | Patrick J. GROHAR | Compositions and methods for the treatment of swi-snf mutant tumors |
| JP7489455B2 (ja) | 2019-10-25 | 2024-05-23 | チャンピン ナショナル ラボラトリー | 哺乳類ｄｎａのメチル化の検出及び分析 |
| WO2021140123A1 (en) | 2020-01-06 | 2021-07-15 | Evaxion Biotech Aps | Vaccines targeting neisseria gonorrhoeae |
| WO2022006286A1 (en) | 2020-06-30 | 2022-01-06 | Lunglife Ai | Methods for detecting lung cancer |
| WO2022047359A1 (en) | 2020-08-31 | 2022-03-03 | Berg Llc | Protein biomarkers for pancreatic cancer |
| WO2022216841A1 (en) | 2021-04-06 | 2022-10-13 | Berg Llc | Protein markers for estrogen receptor (er)-positive luminal a(la)-like and luminal b1 (lb1)-like breast cancer |
| EP4320441A1 (en) | 2021-04-06 | 2024-02-14 | BPGbio, Inc. | Protein markers for estrogen receptor (er)-positive-like and estrogen receptor (er)-negative-like breast cancer |
| US20240230651A1 (en) | 2021-04-06 | 2024-07-11 | Bpgbio, Inc. | Protein markers for the prognosis of breast cancer progression |
| JP2024526293A (ja) | 2021-07-05 | 2024-07-17 | エヴァクシオン・バイオテック・アクティエセルスカブ | 淋菌を標的とするワクチン |
| EP4505182A1 (en) | 2022-04-06 | 2025-02-12 | Larimar Therapeutics, Inc. | Frataxin-sensitive markers for monitoring frataxin replacement therapy |
| EP4532772A1 (en) | 2022-05-24 | 2025-04-09 | Lunglife Ai, Inc. | Methods for detecting circulating genetically abnormal cells |
| WO2023240201A1 (en) | 2022-06-08 | 2023-12-14 | Larimar Therapeutics, Inc. | Frataxin-sensitive markers for monitoring progression and treatment of leigh syndrome |
| KR20250023497A (ko) | 2022-06-10 | 2025-02-18 | 리서치 디벨럽먼트 파운데이션 | 조작된 fcriib 선택적 igg1 fc 변이체 및 이의 용도 |
| WO2025155991A2 (en) | 2024-01-19 | 2025-07-24 | Bpgbio, Inc. | Predictive biomarkers for response to coenzyme q10 treatment in pancreatic cancer |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2169403A (en) | 1985-01-02 | 1986-07-09 | Orion Yhtymae Oy | Identification of nucleic acids |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| US4302204A (en) * | 1979-07-02 | 1981-11-24 | The Board Of Trustees Of Leland Stanford Junior University | Transfer and detection of nucleic acids |
| FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| BG36086A1 (en) * | 1982-01-19 | 1984-09-14 | Glbov | Method for inducing interferon |
| CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
| US4605735A (en) * | 1983-02-14 | 1986-08-12 | Wakunaga Seiyaku Kabushiki Kaisha | Oligonucleotide derivatives |
| NO843838L (no) * | 1983-09-26 | 1985-03-27 | Ortho Diagnostic Systems Inc | Fremgangsmaate for detektering av nukleinsyre |
| US4749647A (en) * | 1984-06-22 | 1988-06-07 | Genetic Systems Corporation | Polymerization-induced separation assay using recognition pairs |
| US4743562A (en) * | 1984-08-21 | 1988-05-10 | The Board Of Trustees Of The Leland Stanford Junior University | Purified human cytomegalovirus protein |
| US4563417A (en) * | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
| US4883750A (en) * | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| CA1272443A (en) * | 1985-02-22 | 1990-08-07 | Nanibhushan Dattagupta | Solution-phase dual hybridization assay for detecting polynucleotide sequences |
| CA1278755C (en) * | 1985-03-15 | 1991-01-08 | William J. Knowles | Labelling of oligonucleotides |
| ES8706823A1 (es) * | 1985-03-28 | 1987-06-16 | Cetus Corp | Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| GB8509367D0 (en) * | 1985-04-12 | 1985-05-15 | Amersham Int Plc | Nucleic acid hybridisation |
| US4751177A (en) * | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
| GB8515276D0 (en) * | 1985-06-17 | 1985-07-17 | Amersham Int Plc | Nucleic acid sequencing |
| CA1284931C (en) * | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
| AU7015287A (en) * | 1986-03-19 | 1987-09-24 | Cetus Corporation | Detection of nucleic acid sequence using liquid hybridization method |
| US4851331A (en) * | 1986-05-16 | 1989-07-25 | Allied Corporation | Method and kit for polynucleotide assay including primer-dependant DNA polymerase |
-
1988
- 1988-02-18 AU AU11937/88A patent/AU622104B2/en not_active Expired
- 1988-02-19 IL IL85480A patent/IL85480A0/xx not_active IP Right Cessation
- 1988-02-19 IL IL10215488A patent/IL102154A/en not_active IP Right Cessation
- 1988-03-01 NZ NZ223700A patent/NZ223700A/en unknown
- 1988-03-03 FI FI880994A patent/FI94429C/fi not_active IP Right Cessation
- 1988-03-09 GR GR880100139A patent/GR1000315B/el not_active IP Right Cessation
- 1988-03-09 DD DD88313526A patent/DD272664A5/de unknown
- 1988-03-10 PT PT86937A patent/PT86937B/pt not_active IP Right Cessation
- 1988-03-10 SE SE8800864A patent/SE500262C2/sv not_active IP Right Cessation
- 1988-03-10 BE BE8800267A patent/BE1003990A5/fr not_active IP Right Cessation
- 1988-03-10 GB GB8805681A patent/GB2202328B/en not_active Expired - Lifetime
- 1988-03-10 JP JP05731088A patent/JP3244500B2/ja not_active Expired - Lifetime
- 1988-03-10 NL NL8800594A patent/NL194922C/nl not_active IP Right Cessation
- 1988-03-10 DE DE3807994A patent/DE3807994C2/de not_active Expired - Lifetime
- 1988-03-10 IT IT8819722A patent/IT1216048B/it active
- 1988-03-10 HU HU881159A patent/HU202583B/hu unknown
- 1988-03-10 CA CA000561135A patent/CA1338207C/en not_active Expired - Fee Related
- 1988-03-10 IE IE69788A patent/IE61664B1/en not_active IP Right Cessation
- 1988-03-10 NO NO881064A patent/NO172909C/no not_active IP Right Cessation
- 1988-03-10 CH CH902/88A patent/CH677285A5/de not_active IP Right Cessation
- 1988-03-10 LU LU87153A patent/LU87153A1/fr unknown
- 1988-03-10 DK DK198801295A patent/DK175384B1/da not_active IP Right Cessation
- 1988-03-10 AT AT0064388A patent/AT395434B/de not_active IP Right Cessation
- 1988-03-10 FR FR8803107A patent/FR2613077A1/fr active Granted
- 1988-03-11 ES ES8800740A patent/ES2006377A6/es not_active Expired
-
1990
- 1990-11-07 US US07/610,470 patent/US5476769A/en not_active Expired - Lifetime
-
1992
- 1992-06-10 IL IL102154A patent/IL102154A0/xx unknown
-
1997
- 1997-08-25 US US08/917,404 patent/US5989817A/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2169403A (en) | 1985-01-02 | 1986-07-09 | Orion Yhtymae Oy | Identification of nucleic acids |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3244500B2 (ja) | 核酸のアッセイ法ならびにそれに用いる試薬およびキット | |
| EP0439222B1 (en) | Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods | |
| AU656514B2 (en) | Detection of a nucleic acid sequence or a change therein | |
| US6090592A (en) | Method for performing amplification of nucleic acid on supports | |
| EP0784701B1 (en) | Method and apparatus for performing amplification of nucleic acid on supports | |
| JP2766003B2 (ja) | ヌクレオチドシークエンス決定方法およびシークエンス決定用キット | |
| EP0427074A2 (en) | Nucleic acid amplification employing transcribable hairpin probe | |
| EP0370694A2 (en) | Diagnostic kit and method using a solid phase capture means for detecting nucleic acids | |
| JPH11506613A (ja) | 競合増幅を用いる核酸配列検出方法 | |
| AU5345790A (en) | Process for nucleic acid detection by binary amplification | |
| JPH11511981A (ja) | 核酸配列における塩基の同定 | |
| AU5359690A (en) | Polymerase chain reaction products incorporating reporter moieties and affinity seperation | |
| WO1989009281A1 (en) | Method for amplifying and detecting nucleic acid in a test liquid | |
| EP0543222A1 (en) | Method for detecting nucleic acids and relative diagnostic kit | |
| JPH05192198A (ja) | 核酸の検出法 | |
| CA2012984A1 (en) | Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid followed by affinity capture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20081026 Year of fee payment: 7 |
|
| EXPY | Cancellation because of completion of term | ||
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20081026 Year of fee payment: 7 |