AU2002227156A1 - Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity - Google Patents

Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity

Info

Publication number
AU2002227156A1
AU2002227156A1 AU2002227156A AU2715602A AU2002227156A1 AU 2002227156 A1 AU2002227156 A1 AU 2002227156A1 AU 2002227156 A AU2002227156 A AU 2002227156A AU 2715602 A AU2715602 A AU 2715602A AU 2002227156 A1 AU2002227156 A1 AU 2002227156A1
Authority
AU
Australia
Prior art keywords
compositions
methods
nucleic acid
acid synthesis
enzymatic nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002227156A
Other languages
English (en)
Inventor
James M. Briggs
Susan H. Hardin
Shiao-Chun Tu
Richard Willson
Gao Xiaolian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Visigen Biotechnologies Inc
Original Assignee
Visigen Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Visigen Biotechnologies Inc filed Critical Visigen Biotechnologies Inc
Publication of AU2002227156A1 publication Critical patent/AU2002227156A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06Pyrimidine radicals
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06Pyrimidine radicals
    • C07H19/10Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
    • C07H19/20Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
AU2002227156A 2000-12-01 2001-12-03 Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity Abandoned AU2002227156A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25076400P 2000-12-01 2000-12-01
US60/250,764 2000-12-01
PCT/US2001/045819 WO2002044425A2 (fr) 2000-12-01 2001-12-03 Synthese d'acides nucleiques d'enzymes, et compositions et methodes modifiant la fidelite d'incorporation de monomeres

Publications (1)

Publication Number Publication Date
AU2002227156A1 true AU2002227156A1 (en) 2002-06-11

Family

ID=22949045

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002227156A Abandoned AU2002227156A1 (en) 2000-12-01 2001-12-03 Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity

Country Status (4)

Country Link
US (12) US7211414B2 (fr)
EP (1) EP1354064A2 (fr)
AU (1) AU2002227156A1 (fr)
WO (1) WO2002044425A2 (fr)

Families Citing this family (426)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5823720A (en) * 1996-02-16 1998-10-20 Bitmoore High precision cutting tools
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
WO2002004680A2 (fr) * 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Determination de sequence en temps reel
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7560254B2 (en) 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
AU2003224836A1 (en) * 2002-04-12 2003-10-27 Stratagene Dual-labeled nucleotides
US20030228611A1 (en) * 2002-05-01 2003-12-11 President And Fellows Of Harvard College Nucleic acid memory device
AU2003263023B2 (en) * 2002-08-29 2008-09-11 Global Life Sciences Solutions Usa Llc Terminal phosphate blocked nucleoside polyphosphates
AU2003263025B2 (en) * 2002-08-29 2009-06-18 Global Life Sciences Solutions Usa Llc Analyte detection
WO2004020604A2 (fr) * 2002-08-29 2004-03-11 Amersham Biosciences Corp Extension d'amorce specifique a l'allele
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
WO2004072304A1 (fr) * 2003-02-05 2004-08-26 Amersham Biosciences Corp Amplification d'acides nucleiques
US7233393B2 (en) * 2004-08-05 2007-06-19 Applera Corporation Signal noise reduction for imaging in biological analysis
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP1846758A2 (fr) * 2005-02-09 2007-10-24 Pacific Biosciences of California, Inc. Compositions nucleotidiques et utilisations de celles-ci
JP2006262829A (ja) * 2005-03-25 2006-10-05 Hitachi High-Technologies Corp 生体試料前処理方法、解析システム及び生体試料前処理要素
WO2007002204A2 (fr) * 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Procedes de pyrosequencage et compositions associees
EP1930422B1 (fr) * 2005-08-19 2013-01-23 Sumitomo Bakelite Company, Ltd. PROCEDE SERVANT A PRODUIRE DES CHAÎNES D'ADNc ET D'ARN ET SUPPORT IMMOBILISANT UN NUCLEOTIDE
US20070190542A1 (en) * 2005-10-03 2007-08-16 Ling Xinsheng S Hybridization assisted nanopore sequencing
WO2007070572A2 (fr) * 2005-12-12 2007-06-21 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Sonde pour le sequencage d’acide nucleique et procedes d’utilisation
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
US20080241951A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
US20080241938A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Automated synthesis or sequencing apparatus and method for making and using same
WO2008027558A2 (fr) 2006-08-31 2008-03-06 Codon Devices, Inc. Assemblage itératif d'acides nucléiques utilisant l'activation de caractères codés par vecteurs
US20080108804A1 (en) * 2006-11-02 2008-05-08 Kabushiki Kaisha Dnaform Method for modifying RNAS and preparing DNAS from RNAS
WO2008151023A2 (fr) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Procédés et compositions pour l'amplification par déplacement multiple d'acides nucléiques
EP2201136B1 (fr) * 2007-10-01 2017-12-06 Nabsys 2.0 LLC Séquençage par nanopore et hybridation de sondes pour former des complexes ternaires et l'alignement de plage variable
AU2008308457A1 (en) * 2007-10-04 2009-04-09 Halcyon Molecular Sequencing nucleic acid polymers with electron microscopy
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 核酸シーケンシング用組成物及び方法
CN103695530B (zh) 2008-07-07 2016-05-25 牛津纳米孔技术有限公司 酶-孔构建体
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
JP5717634B2 (ja) * 2008-09-03 2015-05-13 ナブシス, インコーポレイテッド 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
CA2742298C (fr) 2008-11-03 2019-09-10 The Regents Of The University Of California Procedes de detection d'acides nucleiques resistant a une modification
US8236532B2 (en) 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
WO2010111605A2 (fr) * 2009-03-27 2010-09-30 Nabsys, Inc. La présente invention concerne des dispositifs et des procédés d'analyse de biomolécules et des sondes liées à celles-ci
US8603792B2 (en) 2009-03-27 2013-12-10 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
US8455260B2 (en) * 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
WO2010127304A2 (fr) * 2009-05-01 2010-11-04 Illumina, Inc. Procédés de séquençage
US8246799B2 (en) * 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
ES2628739T3 (es) 2009-10-15 2017-08-03 Ibis Biosciences, Inc. Amplificación por desplazamiento múltiple
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (fr) 2009-11-25 2011-06-03 Gen9, Inc. Dispositifs microfluidiques et procédés pour la synthèse génique
WO2011085075A2 (fr) 2010-01-07 2011-07-14 Gen9, Inc. Assemblage de polynucléotides haute fidélité
EP3151052A1 (fr) 2010-02-01 2017-04-05 Illumina, Inc. Procédés de focalisation et systèmes optiques et ensembles les utilisant
US8481903B2 (en) 2010-03-06 2013-07-09 Alexander Triener Systems, methods, and apparatuses including a moveable optical component for detecting optical signals from a sample
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
PT2556171E (pt) 2010-04-05 2015-12-21 Prognosys Biosciences Inc Ensaios biológicos codificados espacialmente
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
EP2633069B1 (fr) 2010-10-26 2015-07-01 Illumina, Inc. Procédés de séquençage
WO2012061832A1 (fr) 2010-11-05 2012-05-10 Illumina, Inc. Liaison entre des lectures de séquences à l'aide de codes marqueurs appariés
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012064975A1 (fr) 2010-11-12 2012-05-18 Gen9, Inc. Puces à protéines et leurs procédés d'utilisation et de fabrication
ES2548400T3 (es) 2010-11-12 2015-10-16 Gen9, Inc. Métodos y dispositivos para la síntesis de ácidos nucleicos
US8859201B2 (en) 2010-11-16 2014-10-14 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
EP2643484A4 (fr) 2010-11-22 2014-04-16 Univ California Procédés d'identification d'un transcrit cellulaire naissant d'arn
US8932813B2 (en) 2010-12-13 2015-01-13 Life Technologies Corporation Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions
CN103282518B (zh) * 2010-12-17 2016-11-16 纽约哥伦比亚大学理事会 使用经修饰的核苷酸和纳米孔检测的dna边合成边测序
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US9738930B2 (en) 2011-01-28 2017-08-22 The Broad Institute, Inc. Paired end bead amplification and high throughput sequencing
CA2825815C (fr) 2011-01-31 2017-09-05 Illumina, Inc. Procedes pour reduire les dommages occasionnes aux acides nucleiques
WO2012106546A2 (fr) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Cartographie massivement parallèle de contiguïté
WO2012109574A2 (fr) 2011-02-11 2012-08-16 Nabsys, Inc. Procédés de dosage à l'aide de protéines de liaison à l'adn
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2012170936A2 (fr) 2011-06-09 2012-12-13 Illumina, Inc. Cellules à flux modelé utiles pour l'analyse d'acide nucléique
US10364464B2 (en) 2011-08-08 2019-07-30 The Broad Institute, Inc. Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
BR112014003911A2 (pt) 2011-08-19 2017-03-14 Synthetic Genomics Inc método integrado para a identificação de alto rendimento de novas composições pesticidas e seu uso
EP2944693B1 (fr) 2011-08-26 2019-04-24 Gen9, Inc. Compositions et procédés pour ensemble haute fidélité d'acides nucléiques
US10152569B2 (en) 2011-09-26 2018-12-11 Gen-Probe Incorporated Algorithms for sequence determinations
EP2771103B1 (fr) 2011-10-28 2017-08-16 Illumina, Inc. Système et procédé de fabrication de micropuces
US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use
US9200274B2 (en) 2011-12-09 2015-12-01 Illumina, Inc. Expanded radix for polymeric tags
NO2694769T3 (fr) 2012-03-06 2018-03-03
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
WO2013151622A1 (fr) 2012-04-03 2013-10-10 Illumina, Inc. Tête de lecture optoélectronique intégrée et cartouche fluidique utile pour le séquençage d'acides nucléiques
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
EP4001427A1 (fr) 2012-04-24 2022-05-25 Gen9, Inc. Procédés de tri d'acides nucléiques et de clonage in vitro multiplex préparatoire
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
JP6509727B2 (ja) 2012-06-25 2019-05-15 ギンゴー バイオワークス, インコーポレイテッド 核酸アセンブリおよび高処理シークエンシングのための方法
EP2870264A4 (fr) 2012-07-03 2016-03-02 Sloan Kettering Inst Cancer Évaluation quantitative de la reconstitution du répertoire des cellules t chez l'homme après une greffe allogénique de cellules souches hématopoïétiques
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
CA3178340A1 (fr) 2012-08-20 2014-02-27 Illumina, Inc. Procede et systeme de sequencage reposant sur la duree de vie de fluorescence
EP2893729B1 (fr) * 2012-09-07 2017-11-08 Nokia Solutions and Networks Oy Régulation d'utilisation pour un groupe d'abonnés
US9637782B2 (en) 2012-09-28 2017-05-02 Pacific Biosciences Of California, Inc. Charged triplet-state quenchers for mitigation of photo-induced damage
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US10294516B2 (en) 2013-01-18 2019-05-21 Nabsys 2.0 Llc Enhanced probe binding
US9805407B2 (en) 2013-01-25 2017-10-31 Illumina, Inc. Methods and systems for using a cloud computing environment to configure and sell a biological sample preparation cartridge and share related data
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
CA3094792A1 (fr) 2013-03-13 2014-09-18 Illumina, Inc. Procedes et compositions pour le sequencage d'acide nucleique
ES2878525T3 (es) 2013-03-13 2021-11-19 Illumina Inc Dispositivos fluídicos multicapa y procedimientos para su fabricación
CA2898459C (fr) 2013-03-14 2021-02-02 Illumina, Inc. Polymerases modifiees pour l'incorporation amelioree d'analogues nucleotidiques
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
KR101874680B1 (ko) 2013-03-15 2018-07-04 일루미나 케임브리지 리미티드 변형된 뉴클레오사이드 또는 뉴클레오타이드
US9879313B2 (en) 2013-06-25 2018-01-30 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
KR102177225B1 (ko) 2013-07-01 2020-11-10 일루미나, 인코포레이티드 촉매-무함유 표면 작용화 및 중합체 그라프팅
ES2628485T3 (es) 2013-07-03 2017-08-03 Illumina, Inc. Secuenciación mediante síntesis ortogonal
AU2014305889B2 (en) 2013-08-08 2017-06-29 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
AU2014312043A1 (en) 2013-08-30 2016-02-25 Illumina France Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
US10540783B2 (en) 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
US9546398B2 (en) * 2013-11-14 2017-01-17 Agilent Technologies, Inc. Polymerase idling method for single molecule DNA sequencing
EP3715467A1 (fr) 2013-12-03 2020-09-30 Illumina, Inc. Procédé et système permettant d'analyser des données d'image
CN110411998B (zh) 2013-12-10 2022-06-07 伊鲁米那股份有限公司 用于生物或化学分析的生物传感器及其制造方法
CN106414765A (zh) 2013-12-20 2017-02-15 Illumina公司 在片段化的基因组dna样品中保留基因组连接信息
ES2848062T3 (es) 2013-12-23 2021-08-05 Illumina Inc Sustratos estructurados para mejorar la detección de emisiones de luz y métodos relacionados con los mismos
KR102001554B1 (ko) 2014-01-16 2019-07-18 일루미나, 인코포레이티드 고형 지지체 상에서의 앰플리콘 제조 방법 및 시퀀싱
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
NZ723399A (en) 2014-02-18 2020-08-28 Illumina Inc Methods and compositions for dna profiling
US10767219B2 (en) 2014-03-11 2020-09-08 Illumina, Inc. Disposable, integrated microfluidic cartridge and methods of making and using same
CA2947426C (fr) 2014-04-29 2020-01-07 Illumina, Inc. Analyse de l'expression de genes de cellules isolees multiplexees par commutation de matrice et fragmentation et etiquetage (tagmentation)
CA3219413A1 (fr) 2014-05-16 2015-11-19 Illumina, Inc. Techniques de synthese d'acide nucleique
AU2015267189B2 (en) 2014-05-27 2019-12-05 Illumina, Inc. Systems and methods for biochemical analysis including a base instrument and a removable cartridge
WO2015187670A2 (fr) 2014-06-03 2015-12-10 Illumina, Inc. Compositions, systèmes et procédés pour détecter des événements à l'aide d'attaches ancrées ou adjacentes à des nanopores
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
WO2015189636A1 (fr) 2014-06-13 2015-12-17 Illumina Cambridge Limited Procédés et compositions à utiliser pour préparer le séquençage de bibliothèques
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
AU2015279794B2 (en) 2014-06-27 2021-09-30 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
CN112430641A (zh) 2014-06-30 2021-03-02 亿明达股份有限公司 使用单侧转座的方法和组合物
US10605766B2 (en) 2014-07-15 2020-03-31 Illumina, Inc. Biochemically activated electronic device
EP3172321B2 (fr) 2014-07-21 2023-01-04 Illumina, Inc. Enrichissement de polynucléotides à l'aide de systèmes crispr-cas
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
CN107076739B (zh) 2014-08-21 2018-12-25 伊卢米纳剑桥有限公司 可逆表面官能化
WO2016040602A1 (fr) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Séquence au bisulfite à représentation réduite utilisant de l'uracile n-glycosylase (ung) et de l'endonucléase iv
ES2803079T3 (es) 2014-09-12 2021-01-22 Illumina Inc Métodos para detectar la presencia de subunidades de polímeros usando quimioluminiscencia
US20160085910A1 (en) 2014-09-18 2016-03-24 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
WO2016054096A1 (fr) 2014-09-30 2016-04-07 Illumina, Inc. Polymérases modifiées pour l'incorporation améliorée d'analogues de nucléotides
WO2016057950A1 (fr) 2014-10-09 2016-04-14 Illumina, Inc. Procédé et dispositif de séparation de liquides immiscibles, permettant d'isoler efficacement au moins l'un des liquides
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
BR112017007912A2 (pt) 2014-10-17 2018-01-23 Illumina Cambridge Limited transposon de preservação de contiguidade
PT3212684T (pt) 2014-10-31 2020-02-03 Illumina Cambridge Ltd Revestimentos de polímeros e de copolímero de dna
US9828627B2 (en) 2014-11-05 2017-11-28 Illumina Cambridge Limited Reducing DNA damage during sample preparation and sequencing using siderophore chelators
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
WO2016077350A1 (fr) 2014-11-11 2016-05-19 Illumina, Inc. Enrichissement polynucléotidique à l'aide de systèmes crispr-cas
PT3218511T (pt) 2014-11-11 2020-07-23 Illumina Cambridge Ltd Métodos e dispositivos para produção e sequenciamento de grupos monoclonais de ácido nucleico
CN107223161B (zh) 2014-12-15 2022-03-22 亿明达股份有限公司 用于基底上的单分子放置的组合物和方法
PT3256604T (pt) 2015-02-10 2020-05-18 Illumina Inc Métodos e composições para analisar componentes celulares
CN107847930B (zh) 2015-03-20 2020-06-30 亿明达股份有限公司 在竖直或大致竖直的位置中使用的流体盒
DK3274692T3 (da) 2015-03-24 2022-08-29 Illumina Inc Fremgangsmåder til billeddannelse af prøver til biologisk eller kemisk analyse
US20180100192A1 (en) 2015-03-31 2018-04-12 lllumina Cambridge Limited Surface concatemerization of templates
CN113186256A (zh) 2015-04-10 2021-07-30 空间转录公司 生物样本的空间区别、多重核酸分析
EP3696536A1 (fr) 2015-04-14 2020-08-19 Illumina, Inc. Procédé de fabrication d'un substrat et procédé d'analyse de biomolécules aptes à générer des émissions de lumière
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
DK3822365T3 (da) 2015-05-11 2023-02-06 Illumina Inc Platform til opdagelse og analyse af terapeutiske midler
WO2016196358A1 (fr) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Méthodes d'analyse d'acides nucléiques
ES2927121T3 (es) 2015-05-29 2022-11-02 Illumina Inc Portamuestras y sistema de ensayo para realizar reacciones designadas
DK3303614T3 (da) 2015-05-29 2020-05-18 Illumina Cambridge Ltd Forbedret anvendelse af overfladeprimere i klynger
MX2017015517A (es) 2015-06-03 2018-11-09 Illumina Inc Composiciones, sistemas y metodos para secuenciar polinucleotidos usando amarres anclados a polimerasas adyacentes a nanoporos.
GB2556713B (en) 2015-07-06 2021-06-23 Illumina Inc Balanced AC modulation for driving droplet operations electrodes
EP3320111B1 (fr) 2015-07-06 2021-05-05 Illumina Cambridge Limited Préparation d'échantillon pour l'amplification d'acide nucléique
CN107924121B (zh) 2015-07-07 2021-06-08 亿明达股份有限公司 经由纳米压印的选择性表面图案化
US20180207920A1 (en) 2015-07-17 2018-07-26 Illumina, Inc. Polymer sheets for sequencing applications
US10913975B2 (en) 2015-07-27 2021-02-09 Illumina, Inc. Spatial mapping of nucleic acid sequence information
AU2016298541B2 (en) 2015-07-30 2019-10-31 Illumina, Inc. Orthogonal deblocking of nucleotides
PL3334839T3 (pl) 2015-08-14 2021-08-02 Illumina, Inc. Systemy i sposoby wykorzystujące czujniki reagujące na pole magnetyczne do określania informacji genetycznej
CN108474805A (zh) 2015-08-24 2018-08-31 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
SG10201912283RA (en) 2015-08-28 2020-02-27 Illumina Inc Nucleic acid sequence analysis from single cells
WO2017037078A1 (fr) 2015-09-02 2017-03-09 Illumina Cambridge Limited Systèmes et procédés d'amélioration du comportement des gouttelettes dans des systèmes fluidiques
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
JP6936222B2 (ja) 2015-10-22 2021-09-15 イラミーナ インコーポレーテッド 流体装置のための充填剤流体
US11203016B2 (en) 2015-12-01 2021-12-21 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes
AU2016370835B2 (en) 2015-12-17 2020-02-13 Illumina, Inc. Distinguishing methylation levels in complex biological samples
KR20180108578A (ko) 2016-01-11 2018-10-04 일루미나, 인코포레이티드 마이크로 형광 측정기, 유체 시스템, 및 플로우 셀 래치 클램프 모듈을 구비하는 검출 장치
SG11201805787YA (en) 2016-03-24 2018-10-30 Illumina Inc Photonic superlattice-based devices and compositions for use in luminescent imaging, and methods of using the same
PL3377226T3 (pl) 2016-03-28 2021-07-26 Illumina, Inc. Wielopłaszczyznowe mikromacierze
CN109562376B (zh) * 2016-04-04 2022-03-08 纽约哥伦比亚大学董事会 一种基于荧光能量转移的单分子/集群dna合成测序
ES2786974T3 (es) 2016-04-07 2020-10-14 Illumina Inc Métodos y sistemas para la construcción de bibliotecas de ácidos nucleicos normalizadas
WO2017177017A1 (fr) 2016-04-07 2017-10-12 Omniome, Inc. Procédés de quantification d'acides nucléiques cibles et d'identification de variants de séquences
EP4224219A3 (fr) 2016-04-22 2023-08-30 Illumina Inc Dispositifs et compositions à base de structure photonique destinés à être utilisés dans l'imagerie luminescente de sites multiples à l'intérieur d'un pixel, et leurs procédés d'utilisation
CN116397007A (zh) 2016-05-11 2023-07-07 伊鲁米那股份有限公司 使用argonaute系统的多核苷酸富集和扩增
CN109313396B (zh) 2016-05-18 2022-11-22 Illumina公司 使用图案化疏水性表面进行的自组装图案化
DK3488002T3 (da) 2016-07-22 2021-06-21 Univ Oregon Health & Science Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf
WO2018064116A1 (fr) 2016-09-28 2018-04-05 Illumina, Inc. Procédés et systèmes de traitement de données
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc cartridge kit
PL3529380T3 (pl) 2016-10-19 2022-10-24 Illumina, Inc. Sposoby chemicznej ligacji kwasów nukleinowych
US20190348149A1 (en) 2016-11-16 2019-11-14 Illumina, Inc. Validation methods and systems for sequence variant calls
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
EP4119663A1 (fr) 2016-12-09 2023-01-18 The Broad Institute, Inc. Diagnostic basé sur un système effecteur de crispr
CN110325651B (zh) 2016-12-22 2024-03-15 伊鲁米那股份有限公司 具有质量控制示踪剂的阵列
CN110248725B (zh) 2016-12-22 2022-08-02 伊鲁米那股份有限公司 包括树脂膜和图案化的聚合物层的阵列
WO2018119057A2 (fr) 2016-12-22 2018-06-28 Illumina, Inc. Réseau comprenant une amorce de séquençage et une entité non de séquençage
AU2017386515B2 (en) 2016-12-30 2021-08-19 Pacific Biosciences Of California, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
WO2018129314A1 (fr) 2017-01-06 2018-07-12 Illumina, Inc. Correction de phase
WO2018132389A1 (fr) 2017-01-10 2018-07-19 Omniome, Inc. Polymérases modifiées pour réduire la liaison d'adn indépendante des nucléotides
EP3571613A1 (fr) 2017-01-17 2019-11-27 Illumina, Inc. Détermination de variant d'épissage oncogène
AU2018210188B2 (en) 2017-01-18 2023-11-09 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
AU2017394645B2 (en) 2017-01-20 2020-01-23 Pacific Biosciences Of California, Inc. Genotyping by polymerase binding
AU2017394644B2 (en) 2017-01-20 2020-02-06 Pacific Biosciences Of California, Inc. Allele-specific capture of nucleic acids
AU2018210944B2 (en) 2017-01-20 2021-07-22 Pacific Biosciences Of California, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
US11492666B2 (en) 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
AU2018225503A1 (en) 2017-02-21 2018-12-13 Illumina Cambridge Limited Tagmentation using immobilized transposomes with linkers
US11104937B2 (en) 2017-03-15 2021-08-31 The Broad Institute, Inc. CRISPR effector system based diagnostics
US11021740B2 (en) 2017-03-15 2021-06-01 The Broad Institute, Inc. Devices for CRISPR effector system based diagnostics
BR112019019087A2 (pt) 2017-03-15 2020-05-12 The Broad Institute, Inc. Diagnóstico baseado em sistema efetor de crispr para detecção de vírus
US11174515B2 (en) 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
SG10202109852WA (en) 2017-03-20 2021-10-28 Illumina Inc Methods and compositions for preparing nucleic acid libraries
EP3607087A4 (fr) 2017-04-04 2020-12-30 Omniome, Inc. Appareil fluidique et procédés utiles pour des réactions chimiques et biologiques
ES2929837T3 (es) 2017-04-23 2022-12-02 Illumina Cambridge Ltd Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácidos nucleicos indexados
EP3615671B1 (fr) 2017-04-23 2021-07-21 Illumina Cambridge Limited Compositions et procédés permettant d'améliorer l'identification d'échantillons dans des bibliothèques d'acides nucléiques indexés
EP3615691B1 (fr) 2017-04-23 2021-05-26 Illumina, Inc. Compositions et procédés pour améliorer l'identification d'échantillons dans des bibliothèques d'acides nucléiques indexés
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
AU2018261332A1 (en) 2017-05-01 2019-11-07 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
WO2018208699A1 (fr) 2017-05-08 2018-11-15 Illumina, Inc. Courts adaptateurs universels pour l'indexage d'échantillons de polynucléotides
EP3635136B1 (fr) 2017-06-07 2021-10-20 Oregon Health & Science University Banques de génomes entiers de cellules individuelles pour le séquençage de méthylation
WO2018236631A1 (fr) 2017-06-20 2018-12-27 Illumina, Inc. Procédés et compositions pour résoudre les inefficacités dans des réactions d'amplification
EP3655153B1 (fr) 2017-07-18 2020-09-16 Omniome, Inc. Procédé de modification chimique de surfaces plastiques
US20200202977A1 (en) 2017-07-31 2020-06-25 Illumina, Inc. Sequencing system with multiplexed biological sample aggregation
WO2019028047A1 (fr) 2017-08-01 2019-02-07 Illumina, Inc Indexation spatiale de matériel génétique et préparation de pharmacothèque à l'aide de billes d'hydrogel et de cellules d'écoulement
US20220411859A1 (en) 2017-08-01 2022-12-29 Illumina, Inc. Hydrogel beads for nucleotide sequencing
JP6998404B2 (ja) 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 標的ヌクレオチド配列の富化及び決定方法
US10858701B2 (en) 2017-08-15 2020-12-08 Omniome, Inc. Scanning apparatus and method useful for detection of chemical and biological analytes
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
NZ759818A (en) 2017-10-16 2022-04-29 Illumina Inc Semi-supervised learning for training an ensemble of deep convolutional neural networks
JP2020525887A (ja) 2017-10-16 2020-08-27 イルミナ インコーポレイテッド ディープラーニングベースのスプライス部位分類
WO2019136388A1 (fr) 2018-01-08 2019-07-11 Illumina, Inc. Systèmes et dispositifs de séquençage à haut débit avec détection basée sur un semi-conducteur
SG11201911764VA (en) 2018-01-08 2020-01-30 Illumina Inc High-throughput sequencing with semiconductor-based detection
JP6862581B2 (ja) 2018-01-15 2021-04-21 イルミナ インコーポレイテッド 深層学習ベースのバリアント分類器
AU2019213047A1 (en) 2018-01-29 2020-07-16 Massachusetts Institute Of Technology CRISPR effector system based diagnostics
KR20200139671A (ko) 2018-02-06 2020-12-14 옴니옴 인코포레이티드 핵산 프라이머 연장을 위한 조성물 및 기술
JP7027459B2 (ja) 2018-02-13 2022-03-01 イルミナ インコーポレイテッド ヒドロゲルビーズを用いるdna配列決定
JP6974504B2 (ja) 2018-04-02 2021-12-01 イルミナ インコーポレイテッド 配列に基づく遺伝子試験のための対照を作製するための組成物及び方法
US20190318806A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant Classifier Based on Deep Neural Networks
CA3097583A1 (fr) 2018-04-19 2019-10-24 Omniome, Inc. Amelioration de la precision d'appels de base dans des procedes de sequencage d'acide nucleique
JP2020530258A (ja) 2018-04-20 2020-10-22 イルミナ インコーポレイテッド 単一細胞を封入する方法、封入された細胞およびその使用
JP2021521820A (ja) 2018-04-26 2021-08-30 オムニオム インコーポレイテッドOmniome, Inc. 核酸−ヌクレオチド−ポリメラーゼ複合体を安定化するための方法及び組成物
CN111065645A (zh) 2018-05-15 2020-04-24 伊鲁米纳公司 用于表面结合寡核苷酸的化学裂解和脱保护的组合物和方法
CN110785499A (zh) 2018-05-25 2020-02-11 伊鲁米那股份有限公司 对先兆子痫具有特异性的循环rna标识
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
AU2019276719A1 (en) 2018-05-31 2020-11-26 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing
US20210102194A1 (en) 2018-06-04 2021-04-08 Illumina, Inc. High-throughput single-cell transcriptome libraries and methods of making and of using
US20200251183A1 (en) 2018-07-11 2020-08-06 Illumina, Inc. Deep Learning-Based Framework for Identifying Sequence Patterns that Cause Sequence-Specific Errors (SSEs)
CN112567048A (zh) 2018-07-24 2021-03-26 欧姆尼欧美公司 三元复合物种类的连续形成
WO2020033601A1 (fr) 2018-08-07 2020-02-13 The Broad Institute, Inc. Nouveaux systèmes et enzymes cas12b
BR112021002779A2 (pt) 2018-08-15 2021-05-04 Illumina, Inc. composições e métodos para melhorar o enriquecimento de bibliotecas
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US10731141B2 (en) 2018-09-17 2020-08-04 Omniome, Inc. Engineered polymerases for improved sequencing
US20210396756A1 (en) 2018-10-03 2021-12-23 The Broad Institute, Inc. Crispr effector system based diagnostics for hemorrhagic fever detection
KR102165734B1 (ko) 2018-10-15 2020-10-14 일루미나, 인코포레이티드 심층 컨볼루션 신경망을 사전 훈련시키기 위한 심층 학습 기반 기술
CN113272449B (zh) 2018-10-26 2024-03-12 Illumina公司 调整聚合物小珠以进行dna处理
IL299237A (en) 2018-10-31 2023-02-01 Illumina Inc Polymerases, preparations and methods of use
NL2022043B1 (en) 2018-11-21 2020-06-03 Akershus Univ Hf Tagmentation-Associated Multiplex PCR Enrichment Sequencing
JP2022513561A (ja) 2018-11-30 2022-02-09 イルミナ インコーポレイテッド 単一アッセイを使用した複数の分析物の分析
EP3891304A1 (fr) 2018-12-04 2021-10-13 Omniome, Inc. Fluides à phase mixte pour séquençage d'acide nucléique et autres essais analytiques
SG11202012559SA (en) 2018-12-05 2021-01-28 Illumina Inc Polymerases, compositions, and methods of use
KR20210098842A (ko) 2018-12-05 2021-08-11 일루미나 케임브리지 리미티드 브릿지 증폭에 의한 클러스터 생성을 위한 방법 및 조성물
EP3894590A2 (fr) 2018-12-10 2021-10-20 10X Genomics, Inc. Procédés d'utilisation de réseaux maître/copie pour la détection spatiale
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
GB201820341D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen
CN112639126A (zh) 2018-12-14 2021-04-09 伊卢米纳剑桥有限公司 降低测序期间用未标记的核苷酸的定相
CN112639125A (zh) 2018-12-17 2021-04-09 伊卢米纳剑桥有限公司 用于多核苷酸测序的组合物
CN112654714A (zh) 2018-12-17 2021-04-13 伊卢米纳剑桥有限公司 用于测序的引物寡核苷酸
CA3103633A1 (fr) 2018-12-18 2020-06-25 Illumina Cambridge Limited Procedes et compositions pour sequencage d'extremites appariees a l'aide d'une amorce de surface unique
WO2020132103A1 (fr) 2018-12-19 2020-06-25 Illumina, Inc. Procédés d'amélioration de la priorité de la clonalité d'un ensemble de polynucléotides
US11041199B2 (en) 2018-12-20 2021-06-22 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
MX2021003625A (es) 2019-01-11 2021-05-27 Illumina Cambridge Ltd Complejos de transposoma ligados a superficie de complejo.
EP3924513B1 (fr) 2019-02-14 2023-04-12 Pacific Biosciences of California, Inc. Atténuation d'impacts défavorables de systèmes de détection sur des acides nucléiques et d'autres analytes biologiques
EP3927467A4 (fr) 2019-02-20 2022-12-14 Pacific Biosciences of California, Inc. Appareil de balayage et procédés permettant de détecter des analytes chimiques ou biologiques
US20220033805A1 (en) 2019-03-01 2022-02-03 Sanjay Srivatsan High-throughput single-nuclei and single-cell libraries and methods of making and of using
WO2020191387A1 (fr) 2019-03-21 2020-09-24 Illumina, Inc. Appel de base à base d'intelligence artificielle
US11347965B2 (en) 2019-03-21 2022-05-31 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
WO2020243579A1 (fr) 2019-05-30 2020-12-03 10X Genomics, Inc. Procédés de détection de l'hétérogénéité spatiale d'un échantillon biologique
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
BR112021012755A2 (pt) 2019-07-12 2022-04-26 Illumina Cambridge Ltd Composições e métodos para preparar bibliotecas de sequenciamento de ácidos nucleicos usando crispr/cas9 imobilizadas em um suporte sólido
KR20220031539A (ko) 2019-07-12 2022-03-11 일루미나 케임브리지 리미티드 전기영동을 이용한 핵산 라이브러리 제조
WO2021011803A1 (fr) 2019-07-16 2021-01-21 Omniome, Inc. Acides nucléiques synthétiques ayant des structures non naturelles
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
TW202124406A (zh) 2019-09-10 2021-07-01 美商歐姆尼歐美公司 核苷酸之可逆修飾
WO2021050962A1 (fr) 2019-09-11 2021-03-18 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Détection et classification de cancers
WO2021076152A1 (fr) 2019-10-18 2021-04-22 Omniome, Inc. Procédés et compositions pour le coiffage d'acides nucléiques
WO2021091611A1 (fr) 2019-11-08 2021-05-14 10X Genomics, Inc. Agents de capture d'analytes marqués spatialement pour le multiplexage d'analytes
EP4025711A2 (fr) 2019-11-08 2022-07-13 10X Genomics, Inc. Amélioration de la spécificité de la liaison d'un analyte
EP4055155A1 (fr) 2019-11-08 2022-09-14 Pacific Biosciences of California, Inc. Polymérases modifiées pour un séquençage amélioré par liaison
CN113677807A (zh) 2019-11-22 2021-11-19 因美纳有限公司 先兆子痫特异性的循环rna标记
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
EP4010489A1 (fr) 2019-12-04 2022-06-15 Illumina, Inc. Préparation de bibliothèques de séquençage d'adn pour la détection d'agents pathogènes d'adn dans le plasma
MX2021011847A (es) 2019-12-19 2021-11-17 Illumina Inc Genotecas de celulas unicas de alto rendimiento y metodos para elaborarlas y usarlas.
FI3891300T3 (fi) 2019-12-23 2023-05-10 10X Genomics Inc Menetelmät spatiaalista analyysiä varten rna-templatoitua ligaatiota käyttäen
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
WO2021158511A1 (fr) 2020-02-04 2021-08-12 Omniome, Inc. Cuves à circulation et procédés pour leur fabrication et leur utilisation
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
IL295560A (en) 2020-02-20 2022-10-01 Illumina Inc An artificial intelligence-based many-to-many base reader
US20210265016A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Data Compression for Artificial Intelligence-Based Base Calling
US20210265015A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Hardware Execution and Acceleration of Artificial Intelligence-Based Base Caller
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
EP4114966A1 (fr) 2020-03-03 2023-01-11 Pacific Biosciences Of California, Inc. Procédés et compositions de séquençage d'acides nucléiques bicaténaires
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
JP2023520203A (ja) 2020-03-30 2023-05-16 イルミナ インコーポレイテッド 核酸ライブラリを調製するための方法及び組成物
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
CN115836135A (zh) 2020-05-05 2023-03-21 加利福尼亚太平洋生物科学股份有限公司 用于修饰聚合酶-核酸复合物的组合物和方法
EP4150065A2 (fr) 2020-05-12 2023-03-22 Illumina Inc Génération d'acides nucléiques avec des bases modifiées au moyen de désoxynucléotidyl transférase terminale recombinante
WO2021236929A1 (fr) 2020-05-22 2021-11-25 10X Genomics, Inc. Mesure spatio-temporelle simultanée de l'expression génique et de l'activité cellulaire
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (fr) 2020-05-26 2021-12-02 10X Genomics, Inc. Procédé de réinitialisation d'un réseau
CN116249785A (zh) 2020-06-02 2023-06-09 10X基因组学有限公司 用于抗原-受体的空间转录组学
EP4025692A2 (fr) 2020-06-02 2022-07-13 10X Genomics, Inc. Procédés de banques d'acides nucléiques
WO2021252499A1 (fr) 2020-06-08 2021-12-16 10X Genomics, Inc. Méthodes de détermination de marge chirurgicale et méthodes d'utilisation associées
WO2021252617A1 (fr) 2020-06-09 2021-12-16 Illumina, Inc. Procédés pour augmenter le rendement de bibliothèques de séquençage
EP4165207A1 (fr) 2020-06-10 2023-04-19 10X Genomics, Inc. Procédés de détermination d'un emplacement d'un analyte dans un échantillon biologique
AU2021288692A1 (en) 2020-06-11 2023-02-02 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
US11787831B2 (en) 2020-06-22 2023-10-17 Illumina Cambridge Limited Nucleosides and nucleotides with 3′ acetal blocking group
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
CA3182091A1 (fr) 2020-07-02 2022-01-06 Yir-Shyuan WU Procede d'etalonnage de l'efficacite d'ensemencement d'une bibliotheque d'acides nucleiques dans des cuves a circulation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
EP4179109A1 (fr) 2020-07-08 2023-05-17 Illumina, Inc. Billes utilisées en tant que supports pour transposomes
AU2021320307A1 (en) 2020-08-06 2023-02-16 Illumina Cambridge Limited Preparation of RNA and DNA sequencing libraries using bead-linked transposomes
CN116323971A (zh) 2020-08-18 2023-06-23 Illumina公司 核酸的序列特异性靶向转座和选择以及分选
US20220067489A1 (en) 2020-08-28 2022-03-03 Illumina, Inc. Detecting and Filtering Clusters Based on Artificial Intelligence-Predicted Base Calls
CN116323970A (zh) 2020-09-11 2023-06-23 伊鲁米纳剑桥有限公司 使用发夹衔接子从测序文库中富集靶序列的方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
MX2023004461A (es) 2020-10-21 2023-05-03 Illumina Inc Plantillas de secuenciacion que comprenden multiples insertos y composiciones y metodos para mejorar la productividad de secuenciacion.
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (fr) 2020-12-21 2022-06-30 10X Genomics, Inc. Procédés, compositions et systèmes pour capturer des sondes et/ou des codes à barres
EP4277740A1 (fr) 2021-01-13 2023-11-22 Pacific Biosciences of California, Inc. Structuration de surface à l'aide d'un ensemble colloïdal
CN117043352A (zh) 2021-01-29 2023-11-10 伊鲁米纳公司 使用多核苷酸改善流通池的接种效率的方法、组合物和试剂盒
JP2024506304A (ja) 2021-02-04 2024-02-13 イルミナ インコーポレイテッド トランスポソーム結合ビーズ上でのロングインデックス付き連結リード生成
WO2022174054A1 (fr) 2021-02-13 2022-08-18 The General Hospital Corporation Procédés et compositions pour la détection de macromolécules in situ et utilisations correspondantes
WO2022197754A1 (fr) 2021-03-16 2022-09-22 Illumina Software, Inc. Quantification de paramètres de réseau neuronal pour appel de base
EP4301870A1 (fr) 2021-03-18 2024-01-10 10X Genomics, Inc. Capture multiplex de gène et expression de protéines à partir d'un échantillon biologique
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
IL307195A (en) 2021-03-29 2023-11-01 Illumina Inc Improved methods of library preparation
IL307177A (en) 2021-03-29 2023-11-01 Illumina Inc Compounds and methods for assessing DNA damage in the library and normalizing amplicon size bias
WO2022212330A1 (fr) 2021-03-30 2022-10-06 Illumina, Inc. Méthodes améliorées d'adn complémentaire isotherme et préparation de bibliothèque
CN115803816A (zh) 2021-03-31 2023-03-14 因美纳有限公司 具有情境感知的基于人工智能的碱基检出器
EP4314283A1 (fr) 2021-03-31 2024-02-07 Illumina, Inc. Procédés de préparation de banques de séquençage par marquage directionnel utilisant une technologie basée sur les transposons avec des identificateurs moléculaires uniques pour la correction d' erreurs
BR112023019465A2 (pt) 2021-04-02 2023-12-05 Illumina Software Inc Modelo de aprendizado de máquina para detectar uma bolha dentro de uma lâmina de amostra de nucleotídeo para sequenciamento
US20220336054A1 (en) 2021-04-15 2022-10-20 Illumina, Inc. Deep Convolutional Neural Networks to Predict Variant Pathogenicity using Three-Dimensional (3D) Protein Structures
US20220356519A1 (en) 2021-05-10 2022-11-10 Pacific Biosciences Of California, Inc. Single-molecule seeding and amplification on a surface
CN117858959A (zh) 2021-05-10 2024-04-09 加利福尼亚太平洋生物科学股份有限公司 滚环扩增期间的dna扩增缓冲液补充
KR20240009435A (ko) 2021-05-20 2024-01-22 일루미나, 인코포레이티드 합성에 의한 시퀀싱을 위한 조성물 및 방법
US20230016633A1 (en) 2021-06-15 2023-01-19 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
WO2022272260A1 (fr) 2021-06-23 2022-12-29 Illumina, Inc. Compositions, procédés, kits, cartouches et systèmes de séquençage de réactifs
WO2023278184A1 (fr) 2021-06-29 2023-01-05 Illumina, Inc. Procédés et systèmes pour corriger une diaphonie dans un éclairage émis par des sites de réaction
CA3224382A1 (fr) 2021-06-29 2023-01-05 Amirali Kia Organe d'appel de base auto-appris, entraine a l'aide d'oligo-sequences
CN117546245A (zh) 2021-06-29 2024-02-09 因美纳有限公司 用于生成基因组坐标的置信度分类的机器学习模型
AU2022305321A1 (en) 2021-06-29 2024-01-18 Illumina, Inc. Signal-to-noise-ratio metric for determining nucleotide-base calls and base-call quality
US20230005253A1 (en) 2021-07-01 2023-01-05 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
US20230027409A1 (en) 2021-07-13 2023-01-26 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
WO2023003757A1 (fr) 2021-07-19 2023-01-26 Illumina Software, Inc. Extraction d'intensité avec interpolation et adaptation pour appel de base
WO2023004357A1 (fr) 2021-07-23 2023-01-26 Illumina, Inc. Procédés de préparation de surface de substrat pour séquençage d'adn
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
AU2022319125A1 (en) 2021-07-28 2024-01-18 Illumina, Inc. Quality score calibration of basecalling systems
KR20240035413A (ko) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 다중 염기 호출자 모델을 사용한 염기 호출
US20230047225A1 (en) 2021-08-14 2023-02-16 Illumina, Inc. Polymerases, compositions, and methods of use
CN117881795A (zh) 2021-08-17 2024-04-12 因美纳有限公司 用于鉴定甲基化胞嘧啶的方法和组合物
WO2023034489A1 (fr) 2021-09-01 2023-03-09 10X Genomics, Inc. Procédés, compositions et kits pour bloquer une sonde de capture sur un réseau spatial
US20230093253A1 (en) 2021-09-17 2023-03-23 Illumina, Inc. Automatically identifying failure sources in nucleotide sequencing from base-call-error patterns
US20230095961A1 (en) 2021-09-21 2023-03-30 Illumina, Inc. Graph reference genome and base-calling approach using imputed haplotypes
WO2023049215A1 (fr) 2021-09-22 2023-03-30 Illumina, Inc. Appel de base basé sur l'état compressé
WO2023056328A2 (fr) 2021-09-30 2023-04-06 Illumina, Inc. Supports solides et procédés d'appauvrissement et/ou d'enrichissement de fragments de bibliothèque préparés à partir de bioéchantillons
US20230096386A1 (en) 2021-09-30 2023-03-30 Illumina Cambridge Limited Polynucleotide sequencing
CA3232183A1 (fr) 2021-10-11 2023-04-20 Nautilus Subsidiary, Inc. Analyse hautement multiplexable de proteines et de proteomes
AU2022371198A1 (en) 2021-10-20 2024-05-02 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023081485A1 (fr) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Séquençage par étapes d'un polynucléotide à l'aide d'un mélange réactionnel homogène
US20230343415A1 (en) 2021-12-02 2023-10-26 Illumina Software, Inc. Generating cluster-specific-signal corrections for determining nucleotide-base calls
WO2023122363A1 (fr) 2021-12-23 2023-06-29 Illumina Software, Inc. Résumés dynamiques d'état graphique pour séquençage de nucléotides
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
WO2023129764A1 (fr) 2021-12-29 2023-07-06 Illumina Software, Inc. Permutation automatique des versions de modèle d'analyse de variants pour les applications d'analyse génomique
WO2023141154A1 (fr) 2022-01-20 2023-07-27 Illumina Cambridge Limited Procédés de détection de méthylcytosine et d'hydroxyméthylcytosine par séquençage
WO2023164660A1 (fr) 2022-02-25 2023-08-31 Illumina, Inc. Séquences d'étalonnage à des fins de séquençage de nucléotide
WO2023164492A1 (fr) 2022-02-25 2023-08-31 Illumina, Inc. Modèles d'apprentissage automatique destinés à détecter et ajuster des valeurs pour des niveaux de méthylation de nucléotides
WO2023183937A1 (fr) 2022-03-25 2023-09-28 Illumina, Inc. Appel de bases séquence par séquence
US20230314324A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
AU2023248405A1 (en) 2022-04-07 2024-01-04 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2023212490A1 (fr) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systèmes et procédés d'évaluation et d'amélioration de la qualité de dosages moléculaires multiplex
WO2023212601A1 (fr) 2022-04-26 2023-11-02 Illumina, Inc. Modèles d'apprentissage automatique pour sélectionner des sondes oligonucléotidiques pour des technologies de réseau
WO2023209606A1 (fr) 2022-04-29 2023-11-02 Illumina Cambridge Limited Procédés et systèmes d'encapsulation de microsphères lyophilisées
WO2023220627A1 (fr) 2022-05-10 2023-11-16 Illumina Software, Inc. Réseau neuronal adaptatif pour séquençage de nucléotides
WO2023235353A2 (fr) 2022-06-03 2023-12-07 Illumina, Inc. Biomarqueurs d'arn en circulation pour prééclampsie
WO2023239917A1 (fr) 2022-06-09 2023-12-14 Illumina, Inc. Dépendance d'appel de bases envers l'inclinaison de cellule de flux
US20230420080A1 (en) 2022-06-24 2023-12-28 Illumina Software, Inc. Split-read alignment by intelligently identifying and scoring candidate split groups
US20230420082A1 (en) 2022-06-27 2023-12-28 Illumina Software, Inc. Generating and implementing a structural variation graph genome
WO2024006705A1 (fr) 2022-06-27 2024-01-04 Illumina Software, Inc. Génotypage amélioré d'antigène leucocytaire humain (hla)
WO2024006779A1 (fr) 2022-06-27 2024-01-04 Illumina, Inc. Accélérateurs pour un modèle d'imputation de génotypes
WO2024015962A1 (fr) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Adaptateurs en épingle à cheveux asymétriques bloqués
US20240038327A1 (en) 2022-07-26 2024-02-01 Illumina Software, Inc. Rapid single-cell multiomics processing using an executable file
WO2024039516A1 (fr) 2022-08-19 2024-02-22 Illumina, Inc. Détection de la troisième paire de bases de l'adn spécifique de site
WO2024059655A1 (fr) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Caractérisation de l'accessibilité de structures macromoléculaires
WO2024073516A1 (fr) 2022-09-29 2024-04-04 Illumina, Inc. Panneau de référence de variant cible servant à imputer des variants cibles
WO2024073043A1 (fr) 2022-09-30 2024-04-04 Illumina, Inc. Procédés d'utilisation de protéines de liaison cpg dans la cartographie de nucléotides cytosine modifiés
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
WO2024073519A1 (fr) 2022-09-30 2024-04-04 Illumina, Inc. Modèle d'apprentissage automatique pour affiner des appels de variants structuraux
WO2024073047A1 (fr) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine désaminases et procédés d'utilisation dans la cartographie de nucléotides cytosine modifiés
WO2024069581A1 (fr) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Complexes hélicase-cytidine désaminase et procédés d'utilisation
US20240127905A1 (en) 2022-10-05 2024-04-18 Illumina, Inc. Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture
WO2024077152A1 (fr) 2022-10-06 2024-04-11 Illumina, Inc. Sondes servant à appauvrir un petit arn non codant abondant
WO2024077162A2 (fr) 2022-10-06 2024-04-11 Illumina, Inc. Sondes pour améliorer la surveillance d'échantillons de coronavirus
WO2024077202A2 (fr) 2022-10-06 2024-04-11 Illumina, Inc. Sondes pour améliorer la surveillance d'échantillons environnementaux
WO2024081649A1 (fr) 2022-10-11 2024-04-18 Illumina, Inc. Détection et correction de valeurs de méthylation à partir de dosages de séquençage de méthylation

Family Cites Families (358)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
SE429036B (sv) 1979-12-17 1983-08-08 Haustrup Plastic As Behallare med en forslutningsdel
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
NO155316C (no) 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
US5241060A (en) 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
IT1172599B (it) 1983-11-09 1987-06-18 Santarelli Franco Meccanismo per azionare i freni a disco di autoveicoli od altro
US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US5366603A (en) 1984-03-29 1994-11-22 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting useing laser diodes
US6004446A (en) 1984-03-29 1999-12-21 Li-Cor, Inc. DNA Sequencing
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US6207421B1 (en) * 1984-03-29 2001-03-27 Li-Cor, Inc. DNA sequencing and DNA terminators
US6086737A (en) 1984-03-29 2000-07-11 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor
US5230781A (en) 1984-03-29 1993-07-27 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes
US4729947A (en) 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US5571388A (en) 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4739044A (en) 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4764460A (en) * 1985-11-08 1988-08-16 Hoffmann-La Roche Inc. Recombinant sigma NS protein
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
CA1340806C (fr) 1986-07-02 1999-11-02 James Merrill Prober Methode, systeme et reactifs pour le sequencage de l'adn
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5849478A (en) 1986-08-14 1998-12-15 Cashman; Daniel P. Blocked-polymerase polynucleotide immunoassay method and kit
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
CA1338457C (fr) 1986-08-22 1996-07-16 Henry A. Erlich Enzyme thermostable purifiee
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の5−及び6−スクシニミジルカルボキシレ―ト異性体
US5202231A (en) * 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US4793705A (en) 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US5498523A (en) * 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5720928A (en) * 1988-09-15 1998-02-24 New York University Image processing and analysis of individual nucleic acid molecules
US4997928A (en) 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
FR2641793B1 (fr) 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases
KR920700360A (ko) 1989-03-22 1992-02-19 하리크 프리드리히 미끄럼 베어링
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5198060A (en) * 1989-05-15 1993-03-30 Stephen Kurtin Method for replicating solvent-sensitive images
US4979824A (en) 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
IT1231157B (it) 1989-07-20 1991-11-19 Crc Ricerca Chim Nuovi geni ibridi funzionali di bacillus thuringiensis ottenuti mediante ricombinazione in vivo.
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US6984487B1 (en) 1989-08-22 2006-01-10 Hsc Research Development Corporation Cystic fibrosis gene
GB8920571D0 (en) 1989-09-12 1989-10-25 Carr Robert J G Examination of objects of macromolecular size
EP0450060A1 (fr) 1989-10-26 1991-10-09 Sri International Sequen age d'adn
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5274240A (en) 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
US5091652A (en) * 1990-01-12 1992-02-25 The Regents Of The University Of California Laser excited confocal microscope fluorescence scanner and method
IL97222A (en) 1990-02-16 1995-08-31 Orion Yhtymae Oy Method and Responder for Determining Special Changes to Nucleotide
US5401847A (en) 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5227487A (en) 1990-04-16 1993-07-13 Molecular Probes, Inc. Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes
US5079169A (en) * 1990-05-22 1992-01-07 The Regents Of The Stanford Leland Junior University Method for optically manipulating polymer filaments
WO1992010588A1 (fr) 1990-12-06 1992-06-25 Affymax Technologies N.V. Mise en sequence par hybridation d'un acide nucleique cible en une matrice d'oligonucleotides determines
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5578443A (en) 1991-03-06 1996-11-26 Regents Of The University Of Minnesota DNA sequence-based HLA typing method
US5119463A (en) * 1991-04-09 1992-06-02 Abbott Laboratories Compound optical probe employing single optical waveguide
JP3123249B2 (ja) 1991-09-10 2001-01-09 株式会社日立製作所 Dna分子の長さ計測方法および計測装置
US5405747A (en) * 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US6048690A (en) * 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
JPH05244997A (ja) 1992-03-04 1993-09-24 Hitachi Ltd Dnaまたはrnaの塩基配列決定法
AU3816993A (en) 1992-03-19 1993-10-21 Regents Of The University Of California, The Multiple tag labeling method for DNA sequencing
US5470705A (en) 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
GB9210168D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Method of sequencing dna
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
JP2575270B2 (ja) * 1992-11-10 1997-01-22 浜松ホトニクス株式会社 核酸の塩基配列決定方法、単一分子検出方法、その装置及び試料の作成方法
DE69433811T2 (de) 1993-01-07 2005-06-23 Sequenom, Inc., San Diego Dns - sequenzierung durch massenspektronomie
WO1994016104A1 (fr) 1993-01-08 1994-07-21 Ctrc Research Foundation Systeme d'imagerie couleur utilise en biologie moleculaire
WO1994018218A1 (fr) 1993-02-01 1994-08-18 Seq, Ltd. Procedes et appareil de sequençage de l'adn
US5324401A (en) 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
US5403707A (en) * 1993-05-14 1995-04-04 Eastman Kodak Company Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures
US5677196A (en) 1993-05-18 1997-10-14 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
US5424841A (en) * 1993-05-28 1995-06-13 Molecular Dynamics Apparatus for measuring spatial distribution of fluorescence on a substrate
US5473060A (en) 1993-07-02 1995-12-05 Lynx Therapeutics, Inc. Oligonucleotide clamps having diagnostic applications
US5706805A (en) * 1993-08-12 1998-01-13 Trustees Of Dartmouth College Apparatus and methodology for determining oxygen tension in biological systems
US5494030A (en) * 1993-08-12 1996-02-27 Trustees Of Dartmouth College Apparatus and methodology for determining oxygen in biological systems
US5804561A (en) 1993-08-16 1998-09-08 Hsia; Jen-Chang Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules
US5824781A (en) 1993-08-16 1998-10-20 Hsia; Jen-Chang Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules
US5741893A (en) * 1993-08-16 1998-04-21 Hsia; Jen-Chang Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules
US5807831A (en) 1993-08-16 1998-09-15 Hsia; Jen-Chang Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules
US6458758B1 (en) 1993-08-16 2002-10-01 Synzyme Technologies, Inc. Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules
US5725839A (en) * 1993-08-16 1998-03-10 Hsia; Jen-Chang Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules for ERI or MRI
US5817632A (en) 1993-08-16 1998-10-06 Hsia; Jen-Chang Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules
US5840701A (en) 1993-08-16 1998-11-24 Hsia; Jen-Chang Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules
WO1995006138A1 (fr) * 1993-08-25 1995-03-02 The Regents Of The University Of California Procede microscopique pour la detection de micro-mouvements
US5470710A (en) 1993-10-22 1995-11-28 University Of Utah Automated hybridization/imaging device for fluorescent multiplex DNA sequencing
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
EP0730645A1 (fr) * 1993-11-26 1996-09-11 Btg International Limited Adn ameliorant la traduction
JPH07203998A (ja) 1994-01-26 1995-08-08 Hamamatsu Photonics Kk 核酸塩基配列決定方法
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5869255A (en) * 1994-02-01 1999-02-09 The Regents Of The University Of California Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US6593148B1 (en) 1994-03-01 2003-07-15 Li-Cor, Inc. Cyanine dye compounds and labeling methods
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5459325A (en) 1994-07-19 1995-10-17 Molecular Dynamics, Inc. High-speed fluorescence scanner
EP1211315A1 (fr) * 1994-07-29 2002-06-05 Innogenetics N.V. Vecteurs récombinants pour la production de protéines d'enveloppe de HCV
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
SE9500342D0 (sv) * 1995-01-31 1995-01-31 Marek Kwiatkowski Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
US5601982A (en) * 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
WO1996027025A1 (fr) 1995-02-27 1996-09-06 Ely Michael Rabani Dispositif, composes, algorithmes, et procedes de caracterisation et manipulation avec parallelisme moleculaire
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5538898A (en) * 1995-03-16 1996-07-23 International Business Machines Corporation Method suitable for identifying a code sequence of a biomolecule
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) * 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
WO1996030508A1 (fr) 1995-03-24 1996-10-03 Ely Michael Rabani Assemblage d'objets moleculaires et supramoleculaires complexes, dispositifs ainsi obtenus et leurs utilisations
EP0735144B1 (fr) * 1995-03-28 2002-06-05 Japan Science and Technology Corporation Procédé pour l'indexation moléculaire de gènes utilisant des enzymes de restriction
US5961923A (en) 1995-04-25 1999-10-05 Irori Matrices with memories and uses thereof
DE19515552A1 (de) 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
WO1996036737A1 (fr) 1995-05-19 1996-11-21 Ely Michael Rabani Sequençage de polynucleotides parallele et en multiplex
EP0745686A1 (fr) 1995-06-01 1996-12-04 Roche Diagnostics GmbH L'utilisation de l'activité de supression 3'-intrinsèque de l'ADN polymérase
US5776674A (en) 1995-06-05 1998-07-07 Seq, Ltd Chemical biochemical and biological processing in thin films
US5635728A (en) * 1995-06-19 1997-06-03 Denoptix, Inc. Rotating scanner system for reading multiple storage layer radiation screens
DE29509993U1 (de) 1995-06-20 1995-09-14 Textilma Ag Anlage zur Herstellung mindestens eines Wirkbandes mit umhäkelten Gummifäden
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
AU6898296A (en) 1995-08-14 1997-03-12 Ely Michael Rabani Methods and devices for parallel multiplex polynucleotide sequencing
US5661028A (en) 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
US6165765A (en) 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
US5814524A (en) 1995-12-14 1998-09-29 Trustees Of Tufts College Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US5972603A (en) 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US5715534A (en) * 1996-02-23 1998-02-10 Mobley; Gwennette Q. Hats and caps with moveable bills or brims
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US6162931A (en) 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
WO1997039150A1 (fr) 1996-04-15 1997-10-23 University Of Southern California Synthese d'adn marque par fluorophores
US6083695A (en) 1996-04-15 2000-07-04 The University Of Houston Optimized primer library for gene sequencing and method of using same
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5906723A (en) * 1996-08-26 1999-05-25 The Regents Of The University Of California Electrochemical detector integrated on microfabricated capillary electrophoresis chips
AU3829197A (en) 1996-09-03 1998-03-26 Pioneer Hi-Bred International, Inc. Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination
US5830659A (en) 1996-09-13 1998-11-03 University Of Utah Research Foundation Active microtubule-based separations by kinesins
US5723298A (en) * 1996-09-16 1998-03-03 Li-Cor, Inc. Cycle labeling and sequencing with thermostable polymerases
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
EE9600101A (et) 1996-09-27 1998-04-15 Tartu Ülikool Vahend ja meetod DNA nukleotiidide järjestuse määramiseks laserspektroskoopia abil
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US20030104372A1 (en) * 1996-12-23 2003-06-05 Pyrosequencing Ab. Allele specific primer extension
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
FR2758262B1 (fr) 1997-01-14 2004-11-19 Oreal Composition cosmetique ou dermatologique sous forme d'un gel contenant en melange un copolymere associatif, un tensioactif et un agent de conditionnement insoluble
US5804386A (en) 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
US6136543A (en) 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
US6403311B1 (en) * 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
JP3935509B2 (ja) 1997-02-12 2007-06-27 ワイ. チャン,ユージーン ポリマー分析のための方法および製品
ATE269908T1 (de) 1997-04-01 2004-07-15 Manteia S A Methode zur sequenzierung von nukleinsäuren
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US5928869A (en) 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
DE69831899T2 (de) * 1997-06-05 2006-07-13 Kansai Paint Co., Ltd., Amagasaki Phosphorsäuregruppen enthaltende nicht-wässrige Dispersion und Verfahren zu ihrer Verwendung
US5888792A (en) * 1997-07-11 1999-03-30 Incyte Pharmaceuticals, Inc. ATP-dependent RNA helicase protein
BR9812270A (pt) * 1997-07-28 2000-07-18 Medical Biosystems Ltd Análise de sequência de ácido nuclêico
US6245506B1 (en) 1997-07-30 2001-06-12 Bbi Bioseq, Inc. Integrated sequencing device
WO1999010366A1 (fr) 1997-08-29 1999-03-04 Life Technologies, Inc. Polymerases haute fidelite et leur utilisation
US6130101A (en) 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US5932442A (en) 1997-09-23 1999-08-03 Incyte Pharmaceuticals, Inc. Human regulatory molecules
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
CA2305449A1 (fr) 1997-10-10 1999-04-22 President & Fellows Of Harvard College Amplification par replique de reseaux d'acides nucleiques
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
JP2001522607A (ja) 1997-11-12 2001-11-20 マクギル・ユニヴァーシティ Dnaデメチラーゼおよびその治療および診断用途
US6322901B1 (en) * 1997-11-13 2001-11-27 Massachusetts Institute Of Technology Highly luminescent color-selective nano-crystalline materials
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US5936087A (en) * 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6207392B1 (en) * 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
WO1999044045A1 (fr) 1998-02-27 1999-09-02 Massachusetts Institute Of Technology Detection de molecule simple par diffusion raman exalte de surface et utilisations pour le sequençage d'adn et d'arn
AU3487799A (en) 1998-04-09 1999-11-01 University Of Nebraska Board Of Regents Improved live attenuated viruses for use as vectors or vaccines
AU751480B2 (en) 1998-04-29 2002-08-15 Ribozyme Pharmaceuticals, Inc. Nucleoside triphosphates and their incorporation into ribozymes
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
WO1999057321A1 (fr) 1998-05-01 1999-11-11 Arizona Board Of Regents Procede de determination de la sequence nucleotidique d'oligonucleotides et de molecules d'adn
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6287821B1 (en) * 1998-06-11 2001-09-11 Orchid Biosciences, Inc. Nucleotide analogues with 3'-pro-fluorescent fluorophores in nucleic acid sequence analysis
AU4999099A (en) 1998-07-15 2000-02-07 Ljl Biosystems, Inc. Evanescent field illumination devices and methods
DK1102781T3 (da) 1998-07-29 2004-04-05 Max Planck Gesellschaft Nye cofaktorer for methyltransferaser
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
JP2002521064A (ja) 1998-07-30 2002-07-16 ソレックサ リミテッド アレイ生体分子およびシークエンシングにおけるその使用
US6790671B1 (en) 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6210896B1 (en) * 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
CN1323355A (zh) 1998-08-13 2001-11-21 美国吉诺米克斯公司 光学鉴定聚合物
US6280939B1 (en) 1998-09-01 2001-08-28 Veeco Instruments, Inc. Method and apparatus for DNA sequencing using a local sensitive force detector
DE19844931C1 (de) * 1998-09-30 2000-06-15 Stefan Seeger Verfahren zur DNS- oder RNS-Sequenzierung
US6221592B1 (en) * 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
DE69930310T3 (de) 1998-12-14 2009-12-17 Pacific Biosciences of California, Inc. (n. d. Ges. d. Staates Delaware), Menlo Park Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese
GB9828785D0 (en) 1998-12-30 1999-02-17 Amersham Pharm Biotech Ab Sequencing systems
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
ATE508200T1 (de) 1999-02-23 2011-05-15 Caliper Life Sciences Inc Sequenzierung durch inkorporation
US6558945B1 (en) * 1999-03-08 2003-05-06 Aclara Biosciences, Inc. Method and device for rapid color detection
US7270951B1 (en) 1999-03-10 2007-09-18 Asm Scientific, Inc. Method for direct nucleic acid sequencing
US6436640B1 (en) 1999-03-18 2002-08-20 Exiqon A/S Use of LNA in mass spectrometry
IL145422A0 (en) 1999-03-18 2002-06-30 Exiqon As One step sample preparation and detection of nucleic acids in complex in biological samples
CN1361829A (zh) 1999-03-18 2002-07-31 埃克西库恩公司 利用特异性lna引物检测基因突变
AU3567900A (en) 1999-03-30 2000-10-16 Solexa Ltd. Polynucleotide sequencing
GB9907813D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
US20030108867A1 (en) 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
US6399392B1 (en) 1999-04-23 2002-06-04 Molecular Probes, Inc. Xanthene dyes and their application as luminescence quenching compounds
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
DE60024464T2 (de) 1999-05-07 2006-08-03 Roche Diagnostics Gmbh Hohe dichte markierung von dns mit modifizierte oder chromophore tragende nukleotide und benutzte dns polymerasen
FR2793413B1 (fr) * 1999-05-10 2008-11-07 Centre Nat Rech Scient Utilisation d'analogues nucleotidiques agissant comme substrat de la reverse transcriptase pour le traitement des affections faisant intervenir la reverse-transcription
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6635163B1 (en) 1999-06-01 2003-10-21 Cornell Research Foundation, Inc. Entropic trapping and sieving of molecules
US6054316A (en) * 1999-06-25 2000-04-25 Isis Pharmaceuticals Inc. Antisense inhibition of ETs-2 expression
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6762059B2 (en) * 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
AU6771100A (en) 1999-08-13 2001-03-13 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
AU7086800A (en) 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
EP1218543A2 (fr) 1999-09-29 2002-07-03 Solexa Ltd. Sequen age de polynucleotides
WO2001023411A2 (fr) 1999-09-30 2001-04-05 New England Biolabs, Inc. Incorporation de nucleotides modifies par des adn polymerases d'archaeon et methodes associees
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6908736B1 (en) 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
US6372907B1 (en) * 1999-11-03 2002-04-16 Apptera Corporation Water-soluble rhodamine dye peptide conjugates
WO2001032930A1 (fr) 1999-11-04 2001-05-10 California Institute Of Technology Methodes et dispositifs permettant d'analyser des sequences polynucleotidiques
US6399335B1 (en) * 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
WO2001062975A2 (fr) * 2000-02-23 2001-08-30 City Of Hope Polymerisation activee par pyrophosphorolyse (pap): application a l'amplification specifique d'allele et a la determination de sequences d'acide nucleique
AU2001250882A1 (en) 2000-03-20 2001-10-03 Massachusetts Institute Of Technology Inorganic particle conjugates
US6917726B2 (en) * 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
US20020168678A1 (en) 2000-06-07 2002-11-14 Li-Cor, Inc. Flowcell system for nucleic acid sequencing
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
JP2004516810A (ja) 2000-06-07 2004-06-10 リ−コール インコーポレーティッド 電荷スイッチヌクレオチド
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
GB0016472D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
AU2007216737A1 (en) 2000-07-07 2007-10-04 Visigen Biotechnologies, Inc. Real-time sequence determination
WO2002004680A2 (fr) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Determination de sequence en temps reel
US20070172866A1 (en) 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
GB0021977D0 (en) 2000-09-07 2000-10-25 Pyrosequencing Ab Method of sequencing DNA
US20060057565A1 (en) * 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
EP1319047B1 (fr) 2000-09-19 2006-01-04 Li-Cor, Inc. Colorants de cyanine
US7597878B2 (en) 2000-09-19 2009-10-06 Li-Cor, Inc. Optical fluorescent imaging
JP3638516B2 (ja) 2000-09-28 2005-04-13 株式会社日立製作所 核酸検出方法および核酸検出キット
EP1322936A2 (fr) 2000-10-03 2003-07-02 California Institute Of Technology Dispositifs microfluidiques et procedes d'utilisation
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AU2002231934A1 (en) 2001-01-30 2002-08-12 Solexa Ltd. Arrayed polynucleotides and their use in genome analysis
US6514829B1 (en) * 2001-03-12 2003-02-04 Advanced Micro Devices, Inc. Method of fabricating abrupt source/drain junctions
EP1368497A4 (fr) 2001-03-12 2007-08-15 California Inst Of Techn Procedes et appareil d'analyse de sequences de polynucleotide par extension de base asynchrone
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US6320041B1 (en) 2001-04-13 2001-11-20 Trilink Biotechnologies, Inc. Pre-activated carbonyl linkers for the modification of oligonucleotides
WO2002086088A2 (fr) 2001-04-24 2002-10-31 Li-Cor, Inc. Polymerases a activite de commutation de charges et methodes permettant de produire lesdites polymerases
EP1417794A4 (fr) 2001-04-25 2009-09-23 Tekelec Us Procedes et systemes destines a des messages de signalisation a repartition de charge parmi des liaisons de signalisation
GB0112238D0 (en) 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
US7118907B2 (en) 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
WO2002101095A1 (fr) 2001-06-08 2002-12-19 U.S. Genomics, Inc. Procedes et produits permettant d'analyser des acides nucleiques au moyen de la translation de coupure
EP1402071A4 (fr) 2001-06-08 2005-12-14 Us Genomics Inc Procedes et produits pour analyser des acides nucleiques en fonction de l'etat de methylation
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
ATE556845T1 (de) 2001-07-20 2012-05-15 Life Technologies Corp Lumineszierende nanopartikel und ihre herstellung
GB0119719D0 (en) 2001-08-13 2001-10-03 Solexa Ltd DNA sequence analysis
US20030064400A1 (en) * 2001-08-24 2003-04-03 Li-Cor, Inc. Microfluidics system for single molecule DNA sequencing
US7244566B2 (en) 2001-08-29 2007-07-17 Ge Healthcare Bio-Sciences Corp. Analyte detection
JP4318296B2 (ja) 2001-08-29 2009-08-19 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 標識ヌクレオシドポリホスフェート
US7033762B2 (en) 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7223541B2 (en) 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
AU2002365124A1 (en) 2001-09-17 2003-07-09 Biocrystal, Ltd. Nanocrystals
US7205048B2 (en) * 2001-09-17 2007-04-17 Invitrogen Corporation Functionalized fluorescent nanocrystal compositions and methods of making
US7214428B2 (en) 2001-09-17 2007-05-08 Invitrogen Corporation Highly luminescent functionalized semiconductor nanocrystals for biological and physical applications
EP1436424A4 (fr) * 2001-09-18 2005-11-16 Us Genomics Inc Marquage differentiel de polymeres pour une analyse lineaire a haute resolution
US8423294B2 (en) 2001-09-18 2013-04-16 Pathogenetix, Inc. High resolution linear analysis of polymers
AU2003269813A1 (en) 2002-04-16 2003-12-31 Princeton University Gradient structures interfacing microfluidics and nanofluidics, methods for fabrication and uses thereof
US7118871B2 (en) 2002-04-22 2006-10-10 Lawler Joseph F Reagents for monitoring nucleic acid amplification and methods of using same
WO2003091455A1 (fr) 2002-04-23 2003-11-06 U.S.Genomics, Inc. Compositions et procedes relatifs a des sondes d'acides nucleiques a deux bras
US20030235854A1 (en) 2002-05-09 2003-12-25 Chan Eugene Y. Methods for analyzing a nucleic acid
WO2003100101A1 (fr) * 2002-05-28 2003-12-04 U.S. Genomics, Inc. Procedes et appareils d'analyse de polymeres simples
US7282330B2 (en) 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
JP2005533256A (ja) 2002-07-17 2005-11-04 ユー.エス. ジェノミクス, インコーポレイテッド キメラタグを用いるポリマーを分析するための方法と組成物
WO2004020604A2 (fr) 2002-08-29 2004-03-11 Amersham Biosciences Corp Extension d'amorce specifique a l'allele
US7005518B2 (en) * 2002-10-25 2006-02-28 Li-Cor, Inc. Phthalocyanine dyes
DE602004015408D1 (de) 2003-01-23 2008-09-11 Us Genomics Inc Verfahren zur analyse von polymer-populationen
WO2004072304A1 (fr) 2003-02-05 2004-08-26 Amersham Biosciences Corp Amplification d'acides nucleiques
EP1592779A4 (fr) 2003-02-05 2007-12-12 Ge Healthcare Bio Sciences Nucleotides marques par des phosphates terminaux, presentant de nouveaux lieurs
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
EP1620203A2 (fr) 2003-04-10 2006-02-01 U.S. Genomics, Inc. Structures microfluidiques avancees
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
WO2005017205A2 (fr) 2003-08-04 2005-02-24 U. S. Genomics, Inc. Cartographie de l'acide nucleique par une analyse lineaire
WO2005022162A1 (fr) 2003-08-21 2005-03-10 U.S. Genomics, Inc. Points quantiques et leurs procédés d'utilisation
WO2005030815A1 (fr) 2003-09-23 2005-04-07 Dow Global Technologies Inc. Composition de catalyseur autolimitante et procede de polymerisation de propylene
KR100558528B1 (ko) 2003-09-25 2006-03-10 동부아남반도체 주식회사 시모스 이미지 센서 및 그 제조방법
US20050142595A1 (en) 2003-11-07 2005-06-30 U.S. Genomics, Inc. Intercalator FRET donors or acceptors
WO2005047471A2 (fr) 2003-11-07 2005-05-26 U.S. Genomics, Inc. Procede a efficacite amelioree du systeme fret
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005050171A2 (fr) 2003-11-17 2005-06-02 U.S. Genomics, Inc. Procedes et compositions se rapportant a des reactifs centraux simples
JP2007518107A (ja) 2004-01-13 2007-07-05 ユー.エス. ジェノミクス, インコーポレイテッド ポリマーを使用する溶液中の分析物の検出および定量化
US20050196790A1 (en) 2004-02-05 2005-09-08 U.S. Genomics, Inc. Methods for detection and quantitation of minimum length polymers
CA2557177A1 (fr) 2004-02-19 2005-09-01 Stephen Quake Procedes et kits pour analyser des sequences de polynucleotides
US20060046258A1 (en) 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
WO2005089524A2 (fr) 2004-03-19 2005-09-29 U.S. Genomics, Inc. Compositions et procede de detection de molecules simples
US20050239085A1 (en) 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
BRPI0511293A (pt) 2004-05-21 2007-12-04 Halliburton Energy Serv Inc método para medir uma propriedade de formação
US20050260609A1 (en) 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117104A1 (en) 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
CA2566806A1 (fr) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Procedes et dispositifs pour la determination de sequence d'acides nucleiques
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060012793A1 (en) 2004-07-19 2006-01-19 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
EP1786932A4 (fr) * 2004-08-23 2010-10-27 Us Genomics Inc Systemes et procedes de detection et d'analyse de polymeres
JP2008513782A (ja) 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 分子解析のための装置及び方法
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US20070048748A1 (en) 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
WO2007001438A2 (fr) 2004-10-29 2007-01-04 Molecular Probes, Inc. Nanocristaux fluorescents fonctionnalisés et leurs procédés de préparation et d’utilisation
EP1817572A2 (fr) 2004-11-16 2007-08-15 Helicos Biosciences Corporation Train optique et procede de detection et d'analyse tirf de molecule unique
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP1846758A2 (fr) 2005-02-09 2007-10-24 Pacific Biosciences of California, Inc. Compositions nucleotidiques et utilisations de celles-ci
US7130041B2 (en) 2005-03-02 2006-10-31 Li-Cor, Inc. On-chip spectral filtering using CCD array for imaging and spectroscopy
US20060263790A1 (en) 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
WO2007014397A2 (fr) * 2005-07-28 2007-02-01 Helicos Biosciences Corporation Sequençage monomoleculaire de bases consecutives
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7509836B2 (en) * 2005-09-01 2009-03-31 Li-Cor, Inc. Gas flux system chamber design and positioning method
US7405281B2 (en) * 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US20070117102A1 (en) 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
CA2631248C (fr) 2005-11-28 2016-01-12 Pacific Biosciences Of California, Inc. Surfaces uniformes destinees a des substrats de materiau hybride, leurs procedes de production et d'utilisation
US7998717B2 (en) * 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
WO2007070869A2 (fr) 2005-12-16 2007-06-21 Applera Corporation Procede et systeme de sequencage a verrouillage de phase
JP2009526993A (ja) 2006-02-15 2009-07-23 リ−コール インコーポレーティッド 分子撮像のための蛍光フィルタリングシステムおよび方法
US20080076189A1 (en) * 2006-03-30 2008-03-27 Visigen Biotechnologies, Inc. Modified surfaces for the detection of biomolecules at the single molecule level
US20090075252A1 (en) 2006-04-14 2009-03-19 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US7282337B1 (en) * 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US20080241951A1 (en) 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
US20080241938A1 (en) 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Automated synthesis or sequencing apparatus and method for making and using same
US20080076123A1 (en) 2006-09-27 2008-03-27 Helicos Biosciences Corporation Polymerase variants for DNA sequencing
US8202972B2 (en) 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
US7767805B2 (en) 2007-05-03 2010-08-03 Helicos Biosciences Corporation Methods and compositions for sequencing a nucleic acid
US7678894B2 (en) 2007-05-18 2010-03-16 Helicos Biosciences Corporation Nucleotide analogs
US7767400B2 (en) 2008-02-03 2010-08-03 Helicos Biosciences Corporation Paired-end reads in sequencing by synthesis
EP2247741A4 (fr) 2008-02-03 2011-02-23 Helicos Biosciences Corp Eléments de lecture à extrémités appariées dans le séquençage par synthèse
US7790391B2 (en) 2008-03-28 2010-09-07 Helicos Biosciences Corporation Methods of equalizing representation levels of nucleic acid targets
WO2010051434A1 (fr) 2008-10-31 2010-05-06 Helicos Biosciences Corporation Polymérases et leurs procédés d'utilisation

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US20160244822A1 (en) 2016-08-25
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US20140127781A1 (en) 2014-05-08
US9845500B2 (en) 2017-12-19
US20030134807A1 (en) 2003-07-17
US20070172869A1 (en) 2007-07-26
US20100255464A1 (en) 2010-10-07
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US9243284B2 (en) 2016-01-26
US20110184163A1 (en) 2011-07-28
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US20070172819A1 (en) 2007-07-26
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US20100216122A1 (en) 2010-08-26
US20180100191A1 (en) 2018-04-12

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