RU2682270C2 - Рекомбинантный фолликулостимулирующий гормон (ФСГ), включающий альфа-2,3- и альфа-2,6-сиалирование - Google Patents
Рекомбинантный фолликулостимулирующий гормон (ФСГ), включающий альфа-2,3- и альфа-2,6-сиалирование Download PDFInfo
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| RU2010141908/10A RU2537268C2 (ru) | 2008-04-16 | 2009-04-16 | Рекомбинантный фолликулостимулирующий гормон (фсг), включающий альфа-2, 3- и альфа-2, 6-сиалирование |
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| TWI532495B (zh) | 2009-10-05 | 2016-05-11 | 菲瑞茵國際中心股份有限公司 | 藥學製劑 |
| EP2417982A1 (en) | 2010-07-30 | 2012-02-15 | Ferring B.V. | Stabilization of gonadotropins |
| WO2012016576A1 (en) * | 2010-08-04 | 2012-02-09 | Glycotope Gmbh | Improved recombinant human follicle-stimulating hormone |
| DK2621517T4 (da) | 2010-09-29 | 2023-11-20 | Ferring Bv | Sammensætning til anvendelse i behandlingen af infertilitet |
| JP5918697B2 (ja) | 2010-10-15 | 2016-05-18 | Ｊｃｒファーマ株式会社 | 糖鎖の非還元末端がマンノース残基である糖蛋白質の製造方法 |
| ES2693273T3 (es) * | 2011-03-31 | 2018-12-10 | Ferring B.V. | Preparación farmacéutica |
| WO2012168680A1 (en) | 2011-06-06 | 2012-12-13 | Ferring B.V. | Pharmaceutical preparation comprising recombinant fsh |
| JO3092B1 (ar) * | 2011-08-08 | 2017-03-15 | Ferring Bv | مركب لتحفيز مسيطر عليه للمبيض |
| AU2012340501A1 (en) * | 2011-12-19 | 2013-07-11 | Grifols, S.A. | Compositions, methods, and kits for preparing sialylated recombinant proteins |
| US9657310B2 (en) * | 2012-04-27 | 2017-05-23 | Jcr Pharmaceuticals Co., Ltd. | Expression vector |
| EP2824176A1 (en) * | 2013-07-11 | 2015-01-14 | Siamed'xpress | Methods for producing sialylated therapeutic proteins |
| JP6652334B2 (ja) | 2014-05-31 | 2020-02-19 | Ｊｃｒファーマ株式会社 | ウリジンとｎ−アセチル−ｄ−マンノサミンとを含有する培地 |
| JP2018500934A (ja) * | 2014-12-22 | 2018-01-18 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Ｃｍｐ依存性シアリダーゼ活性 |
| HUE054300T2 (hu) | 2015-04-17 | 2021-08-30 | Ferring Bv | FSH-t tartalmazó, meddõség kezelésére szolgáló készítmény |
| WO2016207353A1 (en) | 2015-06-26 | 2016-12-29 | Ferring B.V. | Methods of purification and/or viral inactivation |
| EP3205719A1 (en) * | 2016-02-15 | 2017-08-16 | CEVEC Pharmaceuticals GmbH | Cell lines for producing recombinant glycoproteins with di-antennary n-glycans, methods using the same, and recombinant glycoproteins |
| GB201603280D0 (en) | 2016-02-24 | 2016-04-13 | Ferring Bv | Stable liquid gonadotropin formulation |
| EP3382014A1 (en) | 2017-03-29 | 2018-10-03 | CEVEC Pharmaceuticals GmbH | Recombinant glycoproteins with reduced antennary fucosylation |
| EP3441471A1 (en) | 2017-08-08 | 2019-02-13 | CEVEC Pharmaceuticals GmbH | Use of constitutively active variants of growth factor receptors as selection makers for the generation of stable producer cell lines |
| DK3973982T3 (da) | 2017-09-01 | 2024-09-23 | Ferring Bv | Sammensætning til kontrolleret ovariestimulation |
| HUE071268T2 (hu) | 2018-04-30 | 2025-08-28 | Ferring Bv | Készítmény szabályozott petefészek-stimuláláshoz |
| TWI846743B (zh) | 2018-10-17 | 2024-07-01 | 荷蘭商菲林公司 | 用於控制性卵巢刺激之組成物及方法 |
| WO2021081670A1 (en) * | 2019-11-01 | 2021-05-06 | The University Of British Columbia | Compositions and methods for sialylated mucin-type o-glycosylation of therapeutic proteins |
| TW202237173A (zh) | 2020-12-09 | 2022-10-01 | 荷蘭商菲林公司 | 用於受控的卵巢刺激之組成物及方法 |
| AU2022451605A1 (en) | 2022-04-01 | 2024-09-26 | Ferring B.V. | Mixed protocol for treatment of infertility |
| CA3255772A1 (en) | 2022-05-26 | 2023-11-30 | Ferring B.V. | Compositions and methods of treating infertility in men |
| KR20250033230A (ko) | 2022-07-08 | 2025-03-07 | 훼링 비.브이. | 자궁내 인공수정(iui)을 위한 조성물 및 방법 |
| AR133300A1 (es) | 2023-07-20 | 2025-09-17 | Ferring Bv | Micropartículas cargadas con fármaco de liberación prolongada |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1206302B (it) | 1987-06-26 | 1989-04-14 | Serono Cesare Ist Ricerca | Ormone follicolo-stimolante urinario |
| US5541083A (en) | 1989-10-24 | 1996-07-30 | The Regents Of The University Of California | Method for producing secretable glycosyltransferases and other golgi processing enzymes |
| IL122614A0 (en) | 1995-06-15 | 1998-08-16 | Introgene Bv | Packaging systems for human recombinant adenovirus to be used in gene therapy |
| JP3559652B2 (ja) * | 1996-06-25 | 2004-09-02 | グローリー工業株式会社 | 売上精算システムの管理装置 |
| IL122732A0 (en) * | 1997-01-15 | 1998-08-16 | Akzo Nobel Nv | Liquid gonadotropin-containing formulation its preparation and a device containing same |
| ATE263841T1 (de) | 1997-01-16 | 2004-04-15 | Neose Technologies Inc | Praktische in vitro sialylierung von rekombinanten glykpproteinen |
| JPH1111665A (ja) | 1997-06-28 | 1999-01-19 | Shibuya Kogyo Co Ltd | 無菌エア搬送コンベヤ装置 |
| TWI235158B (en) * | 1997-09-01 | 2005-07-01 | Kirin Brewery | Human thrombopoietin produced by human established cell lines, processes foe preparing the same, and pharmaceutical composition comprising the same |
| CA2372714A1 (en) | 1999-05-07 | 2000-11-16 | Stephen Franks | The use of luteinizing hormone in the induction of pauci-or unifolliculogenesis |
| JP2002011665A (ja) | 2000-06-28 | 2002-01-15 | Sintokogio Ltd | ショットブラスト装置モニタシステム |
| IL140110A0 (en) | 2000-12-05 | 2002-02-10 | Applied Research Systems | Improvement of homogeneity and secretion of recombinant proteins in mammalian systems |
| UA87433C2 (ru) * | 2001-10-22 | 2009-07-27 | Еплайд Рісьорч Системз Ерс Холдінг Н.В. | Гонадотропины для стимулирования фолликулогенеза |
| ES2381104T3 (es) | 2001-10-29 | 2012-05-23 | Crucell Holland B.V. | Métodos y medios para producir proteínas con modificaciones postraduccionales predeterminadas |
| CN100547069C (zh) | 2001-12-07 | 2009-10-07 | 克鲁塞尔荷兰公司 | 病毒、病毒分离物和疫苗的生产 |
| US20040248784A1 (en) | 2003-06-03 | 2004-12-09 | Marco Filicori | Unitary combinations of FSH and hCG |
| EP1694351A2 (en) * | 2003-12-03 | 2006-08-30 | Neose Technologies, Inc. | Glycopegylated follicle stimulating hormone |
| ATE476666T1 (de) | 2004-02-04 | 2010-08-15 | Centre Nat Rech Scient | Verfahren zur identifizierung von glykoformspezifischen antikörpern |
| CN1926242B (zh) * | 2004-02-13 | 2013-01-16 | 格莱克托普有限公司 | 高活性糖蛋白加工条件及其有效生产方法 |
| TWI488640B (zh) | 2008-04-16 | 2015-06-21 | Ferring Int Ct Sa | 藥學製劑 |
| TWI532495B (zh) | 2009-10-05 | 2016-05-11 | 菲瑞茵國際中心股份有限公司 | 藥學製劑 |
| EP2417982A1 (en) | 2010-07-30 | 2012-02-15 | Ferring B.V. | Stabilization of gonadotropins |
| US8790322B2 (en) | 2010-08-03 | 2014-07-29 | King Saud University | Stoma coat |
| WO2012016576A1 (en) * | 2010-08-04 | 2012-02-09 | Glycotope Gmbh | Improved recombinant human follicle-stimulating hormone |
| DK2621517T4 (da) | 2010-09-29 | 2023-11-20 | Ferring Bv | Sammensætning til anvendelse i behandlingen af infertilitet |
| ES2693273T3 (es) | 2011-03-31 | 2018-12-10 | Ferring B.V. | Preparación farmacéutica |
| WO2012168680A1 (en) | 2011-06-06 | 2012-12-13 | Ferring B.V. | Pharmaceutical preparation comprising recombinant fsh |
| JO3092B1 (ar) | 2011-08-08 | 2017-03-15 | Ferring Bv | مركب لتحفيز مسيطر عليه للمبيض |
| AU2015248793A1 (en) | 2014-04-18 | 2016-11-03 | Glycotope Gmbh | Controlled ovarian hyperstimulation with improved recombinant human follicle-stimulating hormone |
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Non-Patent Citations (7)
| Title |
|---|
| BOUSFIELD G. R. et al., Comparison of follicle-stimulating hormone glycosylation microheterogenity by quantitative negative mode nano-electrospray mass spectrometry of peptide-N glycanase-released oligosaccharides, Journal of glycomics & lipidomics, 2015, V. 5, N. 1. * |
| FLACK M. R. et al., Increased biological activity due to basic isoforms in recombinant human follicle-stimulating hormone produced in a human cell line, The Journal of Clinical Endocrinology & Metabolism, 1994, V. 79, N. 3, p.756-760. * |
| FLACK M. R. et al., Increased biological activity due to basic isoforms in recombinant human follicle-stimulating hormone produced in a human cell line, The Journal of Clinical Endocrinology & Metabolism, 1994, V. 79, N. 3, p.756-760. BOUSFIELD G. R. et al., Comparison of follicle-stimulating hormone glycosylation microheterogenity by quantitative negative mode nano-electrospray mass spectrometry of peptide-N glycanase-released oligosaccharides, Journal of glycomics & lipidomics, 2015, V. 5, N. 1. ШПАКОВ А. О. ГЛИКОЗИЛИРОВАНИЕ ГОНАДОТРОПИНОВ КАК ВАЖНЕЙШИЙ МЕХАНИЗМ РЕГУЛЯЦИИ ИХ АКТИВНОСТИ, Российский физиологический журнал им. И.М. Сеченова, 2017, т. 103, N. 9, с.1004-1021. ZAMBRANO E. et al., Receptor binding activity and in vitro biological activity of the human FSH charge isoforms as disclosed by heterologous and homologous assay systems, Endocrine, 1999, V. 10, N. 2, p.113-121. KRONMAN C. et al., Hierarchy of Post-translational Modifications Involved in the Circulatory Longevity of * |
| KRONMAN C. et al., Hierarchy of Post-translational Modifications Involved in the Circulatory Longevity of Glycoproteins DEMONSTRATION OF CONCERTED CONTRIBUTIONS OF GLYCAN SIALYLATION AND SUBUNIT ASSEMBLY TO THE PHARMACOKINETIC BEHAVIOR OF BOVINE ACETYLCHOLINESTERASE, Journal of Biological Chemistry, 2000, V. 275, N. 38, p.29488-29502. * |
| ZAMBRANO E. et al., Receptor binding activity and in vitro biological activity of the human FSH charge isoforms as disclosed by heterologous and homologous assay systems, Endocrine, 1999, V. 10, N. 2, p.113-121. * |
| ШИЯН С. Д. и др. СИАЛИЛИРОВАНИЕ N-УГЛЕВОДНЫХ ЦЕПЕЙ ГЛИКОПРОТЕИНОВ С ПОМОЩЬЮ ИММОБИЛИЗОВАННОЙ ТРАНС-СИАЛИДАЗЫ Trypanosoma cruzi, Биоорганическая химия, 2004, т. 30, N. 4, с.400-408. * |
| ШПАКОВ А. О. ГЛИКОЗИЛИРОВАНИЕ ГОНАДОТРОПИНОВ КАК ВАЖНЕЙШИЙ МЕХАНИЗМ РЕГУЛЯЦИИ ИХ АКТИВНОСТИ, Российский физиологический журнал им. И.М. Сеченова, 2017, т. 103, N. 9, с.1004-1021. * |
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