JP4243349B2 - インフルエンザウイルスの複製のための動物細胞および方法 - Google Patents
インフルエンザウイルスの複製のための動物細胞および方法 Download PDFInfo
- Publication number
- JP4243349B2 JP4243349B2 JP53508997A JP53508997A JP4243349B2 JP 4243349 B2 JP4243349 B2 JP 4243349B2 JP 53508997 A JP53508997 A JP 53508997A JP 53508997 A JP53508997 A JP 53508997A JP 4243349 B2 JP4243349 B2 JP 4243349B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- influenza virus
- infection
- cell
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000712461 unidentified influenza virus Species 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims abstract description 63
- 230000029812 viral genome replication Effects 0.000 title claims description 29
- 210000004102 animal cell Anatomy 0.000 title abstract description 4
- 238000004113 cell culture Methods 0.000 claims abstract description 49
- 229960005486 vaccine Drugs 0.000 claims abstract description 31
- 239000000725 suspension Substances 0.000 claims abstract description 15
- 230000010076 replication Effects 0.000 claims abstract description 14
- 230000012010 growth Effects 0.000 claims abstract description 10
- 239000012679 serum free medium Substances 0.000 claims abstract description 9
- 208000015181 infectious disease Diseases 0.000 claims description 42
- 241000700605 Viruses Species 0.000 claims description 40
- 102000004142 Trypsin Human genes 0.000 claims description 22
- 108090000631 Trypsin Proteins 0.000 claims description 22
- 239000012588 trypsin Substances 0.000 claims description 22
- 230000010412 perfusion Effects 0.000 claims description 21
- 108091005804 Peptidases Proteins 0.000 claims description 12
- 239000004365 Protease Substances 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 9
- 238000002955 isolation Methods 0.000 claims description 9
- 101710154606 Hemagglutinin Proteins 0.000 claims description 8
- 101710093908 Outer capsid protein VP4 Proteins 0.000 claims description 8
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 claims description 8
- 101710176177 Protein A56 Proteins 0.000 claims description 8
- 239000000185 hemagglutinin Substances 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- 102000012479 Serine Proteases Human genes 0.000 claims description 4
- 108010022999 Serine Proteases Proteins 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 239000006285 cell suspension Substances 0.000 claims description 3
- 239000002243 precursor Substances 0.000 claims description 3
- 230000003362 replicative effect Effects 0.000 claims description 3
- 230000009385 viral infection Effects 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 2
- 210000004027 cell Anatomy 0.000 abstract description 132
- 230000008569 process Effects 0.000 abstract description 5
- 239000000470 constituent Substances 0.000 abstract description 2
- 239000002609 medium Substances 0.000 description 29
- 235000013601 eggs Nutrition 0.000 description 15
- 239000000427 antigen Substances 0.000 description 14
- 102000036639 antigens Human genes 0.000 description 14
- 108091007433 antigens Proteins 0.000 description 14
- 238000007792 addition Methods 0.000 description 13
- 102000035195 Peptidases Human genes 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 8
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 7
- 230000010261 cell growth Effects 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 6
- 238000010923 batch production Methods 0.000 description 5
- 239000012141 concentrate Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
- 210000002257 embryonic structure Anatomy 0.000 description 4
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 4
- 206010022000 influenza Diseases 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000003472 neutralizing effect Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000003612 virological effect Effects 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 3
- 239000006143 cell culture medium Substances 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- ZGNLFUXWZJGETL-YUSKDDKASA-N (Z)-[(2S)-2-amino-2-carboxyethyl]-hydroxyimino-oxidoazanium Chemical compound N[C@@H](C\[N+]([O-])=N\O)C(O)=O ZGNLFUXWZJGETL-YUSKDDKASA-N 0.000 description 2
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 241000699800 Cricetinae Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 2
- MLFKVJCWGUZWNV-UHFFFAOYSA-N L-alanosine Natural products OC(=O)C(N)CN(O)N=O MLFKVJCWGUZWNV-UHFFFAOYSA-N 0.000 description 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 208000036142 Viral infection Diseases 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 229950005033 alanosine Drugs 0.000 description 2
- 229960003896 aminopterin Drugs 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000009295 crossflow filtration Methods 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- 230000035931 haemagglutination Effects 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 229960003971 influenza vaccine Drugs 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 230000009401 metastasis Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 238000012770 revaccination Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 239000004017 serum-free culture medium Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 229940031626 subunit vaccine Drugs 0.000 description 2
- 229940104230 thymidine Drugs 0.000 description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 241000991587 Enterovirus C Species 0.000 description 1
- 241001500351 Influenzavirus A Species 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- 208000005647 Mumps Diseases 0.000 description 1
- 238000011785 NMRI mouse Methods 0.000 description 1
- 241000845082 Panama Species 0.000 description 1
- 241000711798 Rabies lyssavirus Species 0.000 description 1
- 241000710799 Rubella virus Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000710771 Tick-borne encephalitis virus Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 229940031567 attenuated vaccine Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000008098 formaldehyde solution Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229940031551 inactivated vaccine Drugs 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 231100000636 lethal dose Toxicity 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229960001226 live attenuated influenza Drugs 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910000000 metal hydroxide Inorganic materials 0.000 description 1
- 150000004692 metal hydroxides Chemical class 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 208000010805 mumps infectious disease Diseases 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000013630 prepared media Substances 0.000 description 1
- 239000002510 pyrogen Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 230000029219 regulation of pH Effects 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000000856 sucrose gradient centrifugation Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 210000000605 viral structure Anatomy 0.000 description 1
- 239000005723 virus inoculator Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/145—Orthomyxoviridae, e.g. influenza virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5252—Virus inactivated (killed)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16151—Methods of production or purification of viral material
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Pulmonology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19612966A DE19612966B4 (de) | 1996-04-01 | 1996-04-01 | MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren |
| DE19612966.4 | 1996-04-01 | ||
| PCT/IB1997/000403 WO1997037000A1 (en) | 1996-04-01 | 1997-04-01 | Animal cells and processes for the replication of influenza viruses |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004233905A Division JP2004331673A (ja) | 1996-04-01 | 2004-08-10 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2005108075A Division JP2005211080A (ja) | 1996-04-01 | 2005-04-04 | インフルエンザウイルスの複製のための動物細胞および方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2000507448A JP2000507448A (ja) | 2000-06-20 |
| JP4243349B2 true JP4243349B2 (ja) | 2009-03-25 |
Family
ID=7790126
Family Applications (7)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP53508997A Expired - Fee Related JP4243349B2 (ja) | 1996-04-01 | 1997-04-01 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2004233905A Withdrawn JP2004331673A (ja) | 1996-04-01 | 2004-08-10 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2005108075A Withdrawn JP2005211080A (ja) | 1996-04-01 | 2005-04-04 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2006147486A Withdrawn JP2006230415A (ja) | 1996-04-01 | 2006-05-26 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2008287206A Withdrawn JP2009034115A (ja) | 1996-04-01 | 2008-11-07 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2011192118A Withdrawn JP2012005502A (ja) | 1996-04-01 | 2011-09-02 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2011224914A Withdrawn JP2012016358A (ja) | 1996-04-01 | 2011-10-12 | インフルエンザウイルスの複製のための動物細胞および方法 |
Family Applications After (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004233905A Withdrawn JP2004331673A (ja) | 1996-04-01 | 2004-08-10 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2005108075A Withdrawn JP2005211080A (ja) | 1996-04-01 | 2005-04-04 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2006147486A Withdrawn JP2006230415A (ja) | 1996-04-01 | 2006-05-26 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2008287206A Withdrawn JP2009034115A (ja) | 1996-04-01 | 2008-11-07 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2011192118A Withdrawn JP2012005502A (ja) | 1996-04-01 | 2011-09-02 | インフルエンザウイルスの複製のための動物細胞および方法 |
| JP2011224914A Withdrawn JP2012016358A (ja) | 1996-04-01 | 2011-10-12 | インフルエンザウイルスの複製のための動物細胞および方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US6455298B1 (enExample) |
| EP (2) | EP2275535A1 (enExample) |
| JP (7) | JP4243349B2 (enExample) |
| AT (1) | ATE482267T1 (enExample) |
| DE (2) | DE19612966B4 (enExample) |
| DK (1) | DK0904351T3 (enExample) |
| ES (1) | ES2352638T3 (enExample) |
| PT (1) | PT904351E (enExample) |
| WO (1) | WO1997037000A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012016358A (ja) * | 1996-04-01 | 2012-01-26 | Novartis Vaccines & Diagnostics Gmbh & Co Kg | インフルエンザウイルスの複製のための動物細胞および方法 |
Families Citing this family (137)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19612967A1 (de) * | 1996-04-01 | 1997-10-02 | Behringwerke Ag | Verfahren zur Vermehrung von Influenzaviren in Zellkultur, sowie die durch das Verfahren erhältlichen Influenzaviren |
| ATE304048T1 (de) * | 1997-10-25 | 2005-09-15 | Roche Diagnostics Gmbh | Suspensions-verpackungszellinien für retrovirusvektoren |
| SK285971B6 (sk) | 1997-12-24 | 2007-12-06 | Duphar International Research B. V. | Spôsob prípravy buniek na výrobu biologických materiálov |
| DE60142506D1 (de) | 2000-03-03 | 2010-08-19 | Chemo Sero Therapeut Res Inst | In serumfreier kultur verwendbare zelle, kultursuspension und verfahren zur virusproduktion als impfstoff unter verwendung der zelle |
| JP3547715B2 (ja) * | 2001-03-06 | 2004-07-28 | 榮 新垣 | ベクター精製用装置及び方法 |
| DE10144903A1 (de) * | 2001-09-12 | 2003-03-27 | Chiron Behring Gmbh & Co | Vermehrung von Viren in Zellkultur |
| DE10144906B4 (de) * | 2001-09-12 | 2013-11-28 | Novartis Vaccines And Diagnostics Gmbh | Verfahren zur großtechnischen Herstellung von Impfstoffen |
| US20030078471A1 (en) * | 2001-10-18 | 2003-04-24 | Foley Frederick J. | Manipulation of an organ |
| US6951752B2 (en) * | 2001-12-10 | 2005-10-04 | Bexter Healthcare S.A. | Method for large scale production of virus antigen |
| CN103540613A (zh) | 2002-04-26 | 2014-01-29 | 米迪缪尼有限公司 | 制备流感病毒的多质粒系统 |
| US20050158342A1 (en) * | 2003-12-23 | 2005-07-21 | Medimmune, Inc. | Multi plasmid system for the production of influenza virus |
| US7465456B2 (en) | 2002-04-26 | 2008-12-16 | Medimmune, Llc | Multi plasmid system for the production of influenza virus |
| EP1597400B8 (en) * | 2003-02-25 | 2013-10-09 | MedImmune, LLC | Methods of producing influenza vaccine compositions |
| US20060110406A1 (en) * | 2003-02-25 | 2006-05-25 | Medimmune Vaccines, Inc. | Refrigerator-temperature stable influenza vaccine compositions |
| DE602004028736D1 (de) * | 2003-06-20 | 2010-09-30 | Microbix Biosystems Inc | Verbesserungen bei der virusproduktion |
| WO2005026333A1 (ja) * | 2003-09-09 | 2005-03-24 | Japan Science And Technology Agency | プロテアーゼを用いた接着性細胞の浮遊培養技術、及び該細胞を宿主として用いてウィルスを生産する方法 |
| US20080254065A1 (en) | 2004-03-09 | 2008-10-16 | Chiron Corporation | Influenza Virus Vaccines |
| BRPI0509493A (pt) * | 2004-04-01 | 2007-09-11 | Alza Corp | aparelho e método para a liberação transdérmica de vacina da influenza |
| NZ551640A (en) * | 2004-05-20 | 2010-05-28 | Id Biomedical Corp | Process for the production of an influenza vaccine |
| EP2578229B1 (en) | 2004-09-09 | 2013-07-10 | Novartis Vaccines and Diagnostics GmbH | Decreasing potential iatrogenic risks associated with vaccine antigens |
| KR101346989B1 (ko) | 2004-10-06 | 2014-02-06 | 메디뮨 엘엘씨 | 냉장 온도 안정성 인플루엔자 백신 조성물 |
| DE102004049290A1 (de) * | 2004-10-09 | 2006-04-20 | Bayer Healthcare Ag | Verfahren zur Herstellung von Virusmaterial |
| EP1807116B1 (en) | 2004-11-03 | 2015-05-06 | Novartis Vaccines and Diagnostics, Inc. | Influenza vaccination |
| KR101316350B1 (ko) | 2004-12-08 | 2013-10-15 | 메디뮨 엘엘씨 | 인플루엔자 백신 조성물의 제조 방법 |
| CN101189326B (zh) * | 2004-12-23 | 2013-06-12 | 米迪缪尼有限公司 | 用于病毒增殖的非致瘤性mdck细胞系 |
| US7572620B2 (en) | 2005-01-11 | 2009-08-11 | Wisconsin Alumni Research Foundation | H3 equine influenza A virus |
| FR2884255B1 (fr) | 2005-04-11 | 2010-11-05 | Vivalis | Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe |
| US20070111309A1 (en) * | 2005-10-04 | 2007-05-17 | Daelli Marcelo G | Vero cell line adapted to grow in suspension |
| CN101365480B (zh) | 2005-11-01 | 2014-11-05 | 诺华疫苗和诊断有限两合公司 | 经由β-丙内酯处理的残留细胞DNA水平降低的细胞衍生病毒疫苗 |
| HUE051122T2 (hu) | 2005-11-04 | 2021-03-01 | Seqirus Uk Ltd | Sejttenyészetben növesztett influenzavírusból elõállított nemvirion anti-géneket tartalmazó adjuvált vakcinák |
| NZ592713A (en) | 2005-11-04 | 2012-12-21 | Novartis Vaccines & Diagnostic | Adjuvanted influenza vaccines including a cytokine-inducing agents other than an agonist of Toll-Like Receptor 9 |
| WO2007052058A1 (en) | 2005-11-04 | 2007-05-10 | Novartis Vaccines And Diagnostics Srl | Influenza vaccines including combinations of particulate adjuvants and immunopotentiators |
| EA014028B1 (ru) | 2005-11-04 | 2010-08-30 | Новартис Вэксинс Энд Диагностикс Срл | Эмульсии, содержащие свободное поверхностно-активное вещество в водной фазе в качестве адъюванта сплит вакцин против гриппа |
| AU2006310163B2 (en) | 2005-11-04 | 2011-09-15 | Seqirus UK Limited | Influenza vaccine with reduced amount of oil-in-water emulsion as adjuvant |
| HUE049580T2 (hu) | 2006-01-27 | 2020-09-28 | Seqirus Uk Ltd | Hemagglutinint és mátrixfehérjéket tartalmazó, influenza elleni oltóanyagok |
| CN101448523A (zh) | 2006-03-24 | 2009-06-03 | 诺华疫苗和诊断有限两合公司 | 无需冷藏储存流感疫苗 |
| WO2007126810A2 (en) | 2006-03-31 | 2007-11-08 | Warf - Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses for vaccines |
| EP2022849A4 (en) * | 2006-05-11 | 2010-10-06 | Chemo Sero Therapeut Res Inst | METHOD FOR PROLIFERATION OF INFLUENZA VIRUS |
| GB0614460D0 (en) | 2006-07-20 | 2006-08-30 | Novartis Ag | Vaccines |
| EP4585610A3 (en) * | 2006-09-11 | 2025-09-24 | Seqirus UK Limited | Making influenza virus vaccines without using eggs |
| EP2069480B1 (en) * | 2006-09-15 | 2014-03-19 | MedImmune, LLC | Mdck cell lines supporting viral growth to high titers and bioreactor process using the same |
| PL2121011T3 (pl) | 2006-12-06 | 2014-10-31 | Novartis Ag | Szczepionki zawierające antygeny czterech szczepów wirusa grypy |
| JP5666905B2 (ja) | 2007-06-18 | 2015-02-12 | メディミューン，エルエルシー | ヘマグルチニンポリペプチド中に変化を有するインフルエンザｂウイルス |
| WO2008156778A2 (en) | 2007-06-18 | 2008-12-24 | Tokiko Watanabe | Influenza m2 protein mutant viruses as live influenza attenuated vaccines |
| CN101784283A (zh) * | 2007-06-27 | 2010-07-21 | 诺华有限公司 | 低添加流感疫苗 |
| EP3327132A3 (en) * | 2007-08-09 | 2018-07-18 | Wyeth LLC | Use of perfusion to enhance production of fed-batch cell culture in bioreactors |
| GB0810305D0 (en) | 2008-06-05 | 2008-07-09 | Novartis Ag | Influenza vaccination |
| US8685654B2 (en) | 2007-12-24 | 2014-04-01 | Novartis Ag | Assays for adsorbed influenza vaccines |
| US8506966B2 (en) | 2008-02-22 | 2013-08-13 | Novartis Ag | Adjuvanted influenza vaccines for pediatric use |
| EA201071086A1 (ru) | 2008-03-18 | 2011-04-29 | Новартис Аг | Усовершенствованный способ получения вакцинных антигенов вируса гриппа |
| TW201012930A (en) * | 2008-06-16 | 2010-04-01 | Intervet Int Bv | Method of replicating viruses in suspension |
| MY172788A (en) | 2008-08-01 | 2019-12-12 | Gamma Vaccines Pty Ltd | Influenza vaccines |
| JP5548207B2 (ja) | 2008-09-24 | 2014-07-16 | メディミューン，エルエルシー | ウイルスの精製方法 |
| CN103952376A (zh) | 2008-09-24 | 2014-07-30 | 米迪缪尼有限公司 | 培养细胞、增殖和纯化病毒的方法 |
| WO2010079081A1 (en) | 2009-01-07 | 2010-07-15 | Glaxosmithkline Biologicals S.A. | Methods for recovering a virus or a viral antigen produced by cell culture |
| CA2750055A1 (en) | 2009-02-06 | 2010-08-12 | Glaxosmithkline Biologicals S.A. | Method for purifying viruses using a density gradient |
| EP2396031A1 (en) | 2009-02-10 | 2011-12-21 | Novartis AG | Influenza vaccines with increased amounts of h3 antigen |
| US20120093859A1 (en) | 2009-02-10 | 2012-04-19 | Novartis Ag | Influenza vaccine regimens for pandemic-associated strains |
| EP3173097A3 (en) | 2009-02-10 | 2017-07-12 | Seqirus UK Limited | Influenza vaccines with reduced amounts of squalene |
| CN102548577A (zh) | 2009-04-27 | 2012-07-04 | 诺华有限公司 | 用于抵抗流感的佐剂疫苗 |
| CN102803515A (zh) | 2009-05-08 | 2012-11-28 | 诺华有限公司 | 用于流感病毒检测的通用测试 |
| ES2394797T3 (es) | 2009-05-21 | 2013-02-05 | Novartis Ag | Genética inversa usando promotores no endógenos de pol l |
| CN103764160A (zh) | 2009-05-29 | 2014-04-30 | 诺华有限公司 | 流感病毒血凝素试验 |
| AU2010277310B2 (en) | 2009-07-31 | 2015-01-15 | Seqirus UK Limited | Reverse genetics systems |
| US20120237536A1 (en) | 2009-09-10 | 2012-09-20 | Novartis | Combination vaccines against respiratory tract diseases |
| JP5871806B2 (ja) | 2009-10-20 | 2016-03-01 | ノバルティス アーゲー | ウイルスレスキューのための改善された逆遺伝学 |
| WO2011056591A1 (en) | 2009-10-26 | 2011-05-12 | Warf - Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses with enhanced replication in vero cells |
| DE102009056884B4 (de) | 2009-12-03 | 2021-03-18 | Novartis Ag | Impfstoff-Adjuvantien und verbesserte Verfahren zur Herstellung derselben |
| EA025622B1 (ru) | 2009-12-03 | 2017-01-30 | Новартис Аг | Способ получения содержащей сквален эмульсии масла |
| DE102009056871A1 (de) | 2009-12-03 | 2011-06-22 | Novartis AG, 4056 | Impfstoff-Adjuvantien und verbesserte Verfahren zur Herstellung derselben |
| MX2012006216A (es) | 2009-12-03 | 2012-07-12 | Novartis Ag | Circulacion de componentes durante la homogenizacion de emulsiones. |
| DE102009056883B4 (de) | 2009-12-03 | 2012-08-16 | Novartis Ag | Impfstoff-Adjuvantien und verbesserte Verfahren zur Herstellung derselben |
| SI2506833T1 (sl) | 2009-12-03 | 2014-07-31 | Novartis Ag | Sklop interakcijske in protitlaäśne komore za mikrofluidiziranje |
| US20120309056A1 (en) | 2010-02-04 | 2012-12-06 | Leon Arnaud | Fed-batch process using concentrated cell culture medium for the efficient production of biologics in eb66 cells |
| AU2011225748A1 (en) | 2010-03-08 | 2012-09-13 | Novartis Ag | Methods of testing for intracellular pathogens |
| US10130697B2 (en) | 2010-03-23 | 2018-11-20 | Wisconsin Alumni Research Foundation (Warf) | Vaccines comprising mutant attenuated influenza viruses |
| ES2562818T3 (es) | 2010-05-03 | 2016-03-08 | Glaxosmithkline Biologicals S.A. | Procedimiento de inactivación del virus de la gripe |
| EP2566323A2 (en) | 2010-05-06 | 2013-03-13 | Novartis AG | Organic peroxide compounds for microorganism inactivation |
| JP5946188B2 (ja) | 2010-05-12 | 2016-07-05 | ノバルティス アーゲー | スクアレンを調製するための改良された方法 |
| EP2571520B1 (en) | 2010-05-21 | 2018-04-04 | Seqirus UK Limited | Influenza virus reassortment method |
| AU2011262312B2 (en) | 2010-06-01 | 2015-05-28 | Novartis Ag | Concentration and lyophilization of influenza vaccine antigens |
| AU2011262309B2 (en) | 2010-06-01 | 2015-08-13 | Seqirus UK Limited | Concentration of influenza vaccine antigens without lyophilization |
| MX337687B (es) * | 2010-08-16 | 2016-03-15 | Cevec Pharmaceuticals Gmbh | Linea celular de amniocitos humanos permanente para la produccion de virus de influenza. |
| US9051361B2 (en) | 2010-08-17 | 2015-06-09 | National Health Research Institutes | Immunogenic compositions and uses thereof |
| US9517205B2 (en) | 2010-08-20 | 2016-12-13 | Seqirus UK Limited | Soluble needle arrays for delivery of influenza vaccines |
| EP3012330A1 (en) | 2010-09-07 | 2016-04-27 | Novartis AG | Generic assays for detection of mammalian reovirus |
| US20140079732A1 (en) | 2011-01-27 | 2014-03-20 | Gamma Vaccines Pty Limited | Combination vaccines |
| JP5999817B2 (ja) | 2011-02-25 | 2016-10-05 | ノバルティス アーゲー | 外因性の内部陽性対照 |
| JP2014527799A (ja) | 2011-08-26 | 2014-10-23 | ダブリュエーアールエフ−ウィスコンシン アラムナイ リサーチ ファウンデーション | 変異型ｐｂ２遺伝子セグメントを有する弱毒化生ワクチンとしてのインフルエンザウイルス |
| GB201216121D0 (en) | 2012-09-10 | 2012-10-24 | Novartis Ag | Sample quantification by disc centrifugation |
| WO2013057715A1 (en) | 2011-10-20 | 2013-04-25 | Novartis Ag | Adjuvanted influenza b virus vaccines for pediatric priming |
| US20140335507A1 (en) | 2011-12-12 | 2014-11-13 | Novartis Ag | Assays for influenza virus hemagglutinins |
| AU2014203051C1 (en) * | 2012-03-02 | 2016-09-01 | Seqirus UK Limited | Influenza Virus Reassortment |
| ES2628301T3 (es) * | 2012-03-02 | 2017-08-02 | Seqirus UK Limited | Recombinación de virus de gripe |
| CA2866465A1 (en) | 2012-03-06 | 2013-09-12 | Crucell Holland B.V. | Improved vaccination against influenza |
| JP6152535B2 (ja) * | 2012-04-16 | 2017-06-28 | 公益財団法人ヒューマンサイエンス振興財団 | 細胞培養組成物及びインフルエンザウイルスの生産方法 |
| HK1208502A1 (en) | 2012-06-04 | 2016-03-04 | Novartis Ag | Improved safety testing |
| GB201218195D0 (en) | 2012-10-10 | 2012-11-21 | Istituto Zooprofilattico Sperimentale Delle Venezie | Composition |
| EP2925356A2 (en) | 2012-12-03 | 2015-10-07 | Novartis AG | Reassortant influenza a viren |
| US10232031B2 (en) | 2013-03-13 | 2019-03-19 | Seqirus UK Limited | Influenza virus reassortment |
| WO2014180999A1 (en) | 2013-05-10 | 2014-11-13 | Novartis Ag | Avoiding narcolepsy risk in influenza vaccines |
| DE202013005130U1 (de) | 2013-06-05 | 2013-09-10 | Novartis Ag | Influenza Virus Reassortierung |
| DE202013005100U1 (de) | 2013-06-05 | 2013-08-26 | Novartis Ag | Influenza Virus Reassortierung |
| BR112015030582A2 (pt) | 2013-06-06 | 2017-08-29 | Novartis Ag | Segmento de hemaglutinina da gripe quimérico e de neuraminidase quimérico, proteína de hemaglutinina quimérica, vírus da gripe rearranjado, métodos para preparação de um vírus da gripe rearranjado e para preparação de uma vacina e sistema de expressão |
| KR101370512B1 (ko) | 2013-06-07 | 2014-03-06 | 재단법인 목암생명공학연구소 | 무단백 배지에서 부유 배양되는 mdck 유래 세포주 및 상기 세포주를 이용하여 바이러스를 증식시키는 방법 |
| WO2015009743A1 (en) | 2013-07-15 | 2015-01-22 | Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses with enhanced replication in mdck or vero cells or eggs |
| US10392603B2 (en) | 2013-08-30 | 2019-08-27 | The Chemo-Sero-Therapeutic Research Institute | Method of viral purification |
| EP3068791B1 (en) | 2013-11-15 | 2020-07-29 | Novartis AG | Removal of residual cell culture impurities |
| TWI743024B (zh) | 2014-06-06 | 2021-10-21 | 美商健臻公司 | 灌注培養方法及其用途 |
| TWI776235B (zh) * | 2014-06-09 | 2022-09-01 | 美商健臻公司 | 種子罐培養法(seed train processes)及其用途 |
| US10053671B2 (en) | 2014-06-20 | 2018-08-21 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
| AU2015252119A1 (en) | 2014-11-07 | 2016-05-26 | Takeda Vaccines, Inc. | Hand, foot, and mouth vaccines and methods of manufacture and use thereof |
| MA40920A (fr) | 2014-11-07 | 2017-09-12 | Takeda Vaccines Inc | Vaccins de la main, du pied et de la bouche, et procédés de fabrication et d'utilisation de ceux-ci |
| ES2861806T3 (es) | 2014-12-02 | 2021-10-06 | Novartis Ag | Fabricación de composiciones que contienen tensioactivo |
| US10633422B2 (en) | 2015-06-01 | 2020-04-28 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication by inhibiting microRNA lec7C binding to influenza viral cRNA and mRNA |
| BR112017028011A2 (pt) | 2015-06-26 | 2018-08-28 | Seqirus Uk Ltd | vacinas de gripe correspondentes antigenicamente |
| US9890363B2 (en) | 2015-07-06 | 2018-02-13 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication for vaccine development |
| CN108027371B (zh) | 2015-07-07 | 2020-08-18 | 思齐乐 | 流感效力试验 |
| WO2017072744A1 (en) * | 2015-10-30 | 2017-05-04 | National Institute Of Infectious Diseases And Vaccinology, National Health Research Institutes | Mdck suspension cell lines in serum-free, chemically-defined media for vaccine production |
| WO2017143236A1 (en) | 2016-02-19 | 2017-08-24 | Yoshihiro Kawaoka | Improved influenza b virus replication for vaccine development |
| AU2017244758B2 (en) | 2016-03-29 | 2022-08-25 | The Research Foundation For Microbial Diseases Of Osaka University | Method for culturing MDCK cells |
| JP2017038622A (ja) * | 2016-11-21 | 2017-02-23 | ウィスコンシン アルムニ リサーチ ファンデイション | Ｈ３型ウマａ型インフルエンザウイルス |
| US11197926B2 (en) | 2017-10-25 | 2021-12-14 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized HA for replication in eggs |
| US11648304B2 (en) | 2017-11-03 | 2023-05-16 | Takeda Vaccines, Inc. | Zika vaccines and immunogenic compositions, and methods of using the same |
| JP7783047B2 (ja) | 2018-08-07 | 2025-12-09 | ウィスコンシン アルムニ リサーチ ファンデイション | 組換えの生物学的に封じ込められたフィロウイルスワクチン |
| JP7655849B2 (ja) | 2018-08-20 | 2025-04-02 | ウィスコンシン アルムニ リサーチ ファンデイション | ヘマグルチニン（ｈａ）タンパク質内の非ドミナントエピトープに対する免疫応答を誘起するためのベクター |
| JP2022527235A (ja) | 2019-01-23 | 2022-06-01 | 義裕 河岡 | インフルエンザウイルスにおける付加的遺伝子に遺伝的安定性を付与する突然変異 |
| EP3921413A1 (en) | 2019-02-08 | 2021-12-15 | Wisconsin Alumni Research Foundation (WARF) | Humanized cell line |
| US11390649B2 (en) | 2019-05-01 | 2022-07-19 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication for vaccine development |
| CN113939311A (zh) | 2019-05-08 | 2022-01-14 | 武田疫苗股份有限公司 | 灭活病毒组合物和寨卡疫苗制剂 |
| US11807872B2 (en) | 2019-08-27 | 2023-11-07 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized HA for replication in eggs |
| MX2022006005A (es) | 2019-11-18 | 2022-10-27 | Seqirus Pty Ltd | Metodo para producir virus de la influenza reagrupados. |
| JP2023511444A (ja) | 2020-01-24 | 2023-03-17 | ウィスコンシン アルムニ リサーチ ファンデイション | 安定化されたｎａを有する組換えインフルエンザウイルス |
| WO2021195410A1 (en) | 2020-03-25 | 2021-09-30 | Wisconsin Alumni Research Foundation (Warf) | Recombinant multivalent influenza viruses |
| US20230218526A1 (en) | 2020-06-30 | 2023-07-13 | Seqirus UK Limited | Cold filtration of oil-in-water emulsion adjuvants |
| EP4476329A1 (en) | 2022-02-09 | 2024-12-18 | Takeda Vaccines, Inc. | Zika vaccines and immunogenic compositions, and methods of using the same |
| CN116376810A (zh) * | 2023-04-26 | 2023-07-04 | 上海奥浦迈生物科技股份有限公司 | 一种mdck细胞无血清贴壁培养驯化的方法 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1278075B (de) | 1964-06-22 | 1968-09-19 | Norden Lab Inc | Verwendung von Gewebekulturen zur Zuechtung von Viren fuer die Gewinnung von Impfstoffen |
| US3616203A (en) * | 1969-12-11 | 1971-10-26 | Norden Lab Inc | Virus culture and method |
| US4064232A (en) | 1974-01-14 | 1977-12-20 | Sandoz Ltd. | Process for isolating the immunogenic components of influenza viruses |
| CA1122527A (en) | 1979-05-15 | 1982-04-27 | Karen K. Brown | Influenza vaccine production in liquid cell culture |
| USRE33164E (en) * | 1979-05-15 | 1990-02-13 | Mobay Corporation | Influenza vaccine production in liquid cell culture |
| US4500513A (en) * | 1979-05-15 | 1985-02-19 | Miles Laboratories, Inc. | Influenza vaccine production in liquid cell culture |
| US5013663A (en) * | 1983-06-15 | 1991-05-07 | American Home Products Corporation | Canine corona virus vaccine |
| US4783411A (en) | 1984-10-22 | 1988-11-08 | Janis Gabliks | Influenza-A virus vaccine from fish cell cultures |
| EP0389925B1 (en) | 1989-03-29 | 1995-12-27 | The Research Foundation Of State University Of New York | A method for purifying an outer membrane protein of Haemophilus influenzae |
| JP2827298B2 (ja) * | 1989-07-10 | 1998-11-25 | 三菱化学株式会社 | 細胞培養担体 |
| AT393277B (de) | 1990-01-04 | 1991-09-25 | Immuno Ag | Verfahren zur herstellung von fruehsommer- meningoenzephalitis-virus (fsme-virus)-antigen |
| JPH0420284A (ja) * | 1990-05-12 | 1992-01-23 | Mitsubishi Kasei Corp | 細胞培養用マイクロキャリア |
| JPH05207873A (ja) * | 1992-01-27 | 1993-08-20 | Mitsubishi Kasei Corp | 付着性動物細胞の培養方法 |
| US5762939A (en) * | 1993-09-13 | 1998-06-09 | Mg-Pmc, Llc | Method for producing influenza hemagglutinin multivalent vaccines using baculovirus |
| US5753489A (en) * | 1994-11-10 | 1998-05-19 | Immuno Ag | Method for producing viruses and vaccines in serum-free culture |
| ES2323490T3 (es) | 1994-11-10 | 2009-07-17 | Baxter Healthcare Sa | Procedimiento de produccion de agentes biologicos en un medio de cultivo sin proteinas. |
| US5756341A (en) * | 1994-11-10 | 1998-05-26 | Immuno Ag | Method for controlling the infectivity of viruses |
| AU694592B2 (en) | 1994-11-16 | 1998-07-23 | St. Jude Children's Research Hospital | Novel replication process |
| US5646033A (en) * | 1994-11-30 | 1997-07-08 | Dyncorp | African green monkey kidney cell lines useful for maintaining viruses and for preparation of viral vaccines |
| JPH11513372A (ja) | 1995-09-22 | 1999-11-16 | コノート ラボラトリーズ リミテッド | パラインフルエンザウイルスの糖タンパクおよびワクチン |
| DE19612967A1 (de) | 1996-04-01 | 1997-10-02 | Behringwerke Ag | Verfahren zur Vermehrung von Influenzaviren in Zellkultur, sowie die durch das Verfahren erhältlichen Influenzaviren |
| DE19612966B4 (de) | 1996-04-01 | 2009-12-10 | Novartis Vaccines And Diagnostics Gmbh & Co. Kg | MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren |
| US6514502B1 (en) * | 1999-01-26 | 2003-02-04 | Schering-Plough Veterinary Corporation | Propagation of bovine cononavirus in chinese hamster ovary cells |
-
1996
- 1996-04-01 DE DE19612966A patent/DE19612966B4/de not_active Expired - Fee Related
-
1997
- 1997-04-01 EP EP10075278A patent/EP2275535A1/en not_active Withdrawn
- 1997-04-01 ES ES97915627T patent/ES2352638T3/es not_active Expired - Lifetime
- 1997-04-01 WO PCT/IB1997/000403 patent/WO1997037000A1/en not_active Ceased
- 1997-04-01 US US09/155,581 patent/US6455298B1/en not_active Expired - Lifetime
- 1997-04-01 AT AT97915627T patent/ATE482267T1/de active
- 1997-04-01 EP EP97915627A patent/EP0904351B1/en not_active Expired - Lifetime
- 1997-04-01 JP JP53508997A patent/JP4243349B2/ja not_active Expired - Fee Related
- 1997-04-01 DE DE69740002T patent/DE69740002D1/de not_active Expired - Lifetime
- 1997-04-01 DK DK97915627.0T patent/DK0904351T3/da active
- 1997-04-01 PT PT97915627T patent/PT904351E/pt unknown
-
2002
- 2002-07-12 US US10/194,784 patent/US6656720B2/en not_active Expired - Lifetime
-
2004
- 2004-08-10 JP JP2004233905A patent/JP2004331673A/ja not_active Withdrawn
-
2005
- 2005-02-15 US US11/057,370 patent/US20050202553A1/en not_active Abandoned
- 2005-04-04 JP JP2005108075A patent/JP2005211080A/ja not_active Withdrawn
-
2006
- 2006-05-26 JP JP2006147486A patent/JP2006230415A/ja not_active Withdrawn
-
2008
- 2008-06-26 US US12/146,689 patent/US20080274141A1/en not_active Abandoned
- 2008-11-07 JP JP2008287206A patent/JP2009034115A/ja not_active Withdrawn
-
2011
- 2011-09-02 JP JP2011192118A patent/JP2012005502A/ja not_active Withdrawn
- 2011-10-12 JP JP2011224914A patent/JP2012016358A/ja not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012016358A (ja) * | 1996-04-01 | 2012-01-26 | Novartis Vaccines & Diagnostics Gmbh & Co Kg | インフルエンザウイルスの複製のための動物細胞および方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20080274141A1 (en) | 2008-11-06 |
| JP2000507448A (ja) | 2000-06-20 |
| HK1019233A1 (en) | 2000-01-28 |
| DK0904351T3 (da) | 2010-11-01 |
| JP2004331673A (ja) | 2004-11-25 |
| US20050202553A1 (en) | 2005-09-15 |
| DE19612966A1 (de) | 1997-10-02 |
| EP2275535A1 (en) | 2011-01-19 |
| PT904351E (pt) | 2010-12-28 |
| DE19612966B4 (de) | 2009-12-10 |
| ES2352638T3 (es) | 2011-02-22 |
| DE69740002D1 (de) | 2010-11-04 |
| WO1997037000A1 (en) | 1997-10-09 |
| EP0904351B1 (en) | 2010-09-22 |
| EP0904351A1 (en) | 1999-03-31 |
| JP2012016358A (ja) | 2012-01-26 |
| US6656720B2 (en) | 2003-12-02 |
| US6455298B1 (en) | 2002-09-24 |
| JP2012005502A (ja) | 2012-01-12 |
| JP2005211080A (ja) | 2005-08-11 |
| JP2009034115A (ja) | 2009-02-19 |
| JP2006230415A (ja) | 2006-09-07 |
| ATE482267T1 (de) | 2010-10-15 |
| US20030073223A1 (en) | 2003-04-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4243349B2 (ja) | インフルエンザウイルスの複製のための動物細胞および方法 | |
| JP5264843B2 (ja) | 細胞培養におけるインフルエンザウイルスの複製の方法、および本方法によって入手可能なインフルエンザウイルス | |
| US10329536B2 (en) | Methods for producing an active constituent of a pharmaceutical or a diagnostic agent in an MDCK cell suspension culture | |
| KR100968141B1 (ko) | 세포 배양물에서 바이러스의 증식 방법 | |
| CN104862267A (zh) | 适应于无血清培养和悬浮培养的mdck来源的细胞系与利用该细胞制备疫苗病毒的方法 | |
| CA2250714C (en) | Animal cells and processes for the replication of influenza viruses | |
| HK1019233B (en) | Animal cells and processes for the replication of influenza viruses | |
| HK1152546A (en) | Animal cells and processes for the replication of influenza viruses | |
| HK1075467B (en) | Process for the replication of influenza viruses in cell culture, and the influenza viruses obtainable by the process | |
| HK1142093B (en) | Process for the replication of influenza viruses in cell culture, and the influenza viruses obtainable by the process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20040507 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20040705 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20040810 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20041005 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20041221 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20050214 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20050913 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20051227 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20060119 |
|
| A912 | Re-examination (zenchi) completed and case transferred to appeal board |
Free format text: JAPANESE INTERMEDIATE CODE: A912 Effective date: 20060202 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20071225 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20071228 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20080623 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20080701 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20080718 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20080730 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20080822 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080828 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20080902 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20081028 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20090105 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 3 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 3 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
| R360 | Written notification for declining of transfer of rights |
Free format text: JAPANESE INTERMEDIATE CODE: R360 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 3 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 3 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 3 |
|
| R370 | Written measure of declining of transfer procedure |
Free format text: JAPANESE INTERMEDIATE CODE: R370 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 3 |
|
| R360 | Written notification for declining of transfer of rights |
Free format text: JAPANESE INTERMEDIATE CODE: R360 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 3 |
|
| R370 | Written measure of declining of transfer procedure |
Free format text: JAPANESE INTERMEDIATE CODE: R370 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 3 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 3 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130109 Year of fee payment: 4 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130109 Year of fee payment: 4 |
|
| LAPS | Cancellation because of no payment of annual fees |