JP2015533210A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015533210A5 JP2015533210A5 JP2015526521A JP2015526521A JP2015533210A5 JP 2015533210 A5 JP2015533210 A5 JP 2015533210A5 JP 2015526521 A JP2015526521 A JP 2015526521A JP 2015526521 A JP2015526521 A JP 2015526521A JP 2015533210 A5 JP2015533210 A5 JP 2015533210A5
- Authority
- JP
- Japan
- Prior art keywords
- specimen
- hydrogel
- tissue
- embedded
- microscope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000017 hydrogel Substances 0.000 claims description 14
- -1 polyethylene copolymer Polymers 0.000 claims description 5
- 229920002635 polyurethane Polymers 0.000 claims description 5
- 239000004814 polyurethane Substances 0.000 claims description 5
- 229920000671 polyethylene glycol diacrylate Polymers 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 239000002202 Polyethylene glycol Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000000834 fixative Substances 0.000 claims description 3
- 229920001223 polyethylene glycol Polymers 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 claims description 2
- 229920000936 Agarose Polymers 0.000 claims description 2
- 229920001661 Chitosan Polymers 0.000 claims description 2
- 102000008186 Collagen Human genes 0.000 claims description 2
- 108010035532 Collagen Proteins 0.000 claims description 2
- 229920002307 Dextran Polymers 0.000 claims description 2
- 108010010803 Gelatin Proteins 0.000 claims description 2
- 239000004952 Polyamide Substances 0.000 claims description 2
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 239000004721 Polyphenylene oxide Substances 0.000 claims description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 229920001436 collagen Polymers 0.000 claims description 2
- 229920000159 gelatin Polymers 0.000 claims description 2
- 239000008273 gelatin Substances 0.000 claims description 2
- 235000019322 gelatine Nutrition 0.000 claims description 2
- 235000011852 gelatine desserts Nutrition 0.000 claims description 2
- 229920002674 hyaluronan Polymers 0.000 claims description 2
- 229960003160 hyaluronic acid Drugs 0.000 claims description 2
- 229920000609 methyl cellulose Polymers 0.000 claims description 2
- 239000001923 methylcellulose Substances 0.000 claims description 2
- 229920002401 polyacrylamide Polymers 0.000 claims description 2
- 229920002647 polyamide Polymers 0.000 claims description 2
- 229920000728 polyester Polymers 0.000 claims description 2
- 229920000570 polyether Polymers 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 229920002338 polyhydroxyethylmethacrylate Polymers 0.000 claims description 2
- 229920001451 polypropylene glycol Polymers 0.000 claims description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 2
- 235000018102 proteins Nutrition 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 41
- 238000001962 electrophoresis Methods 0.000 claims 7
- 238000003384 imaging method Methods 0.000 claims 5
- 210000002569 neuron Anatomy 0.000 claims 5
- 239000008393 encapsulating agent Substances 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 230000000379 polymerizing effect Effects 0.000 claims 4
- 150000003384 small molecules Chemical class 0.000 claims 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 3
- 210000004027 cell Anatomy 0.000 claims 3
- 230000001413 cellular effect Effects 0.000 claims 3
- 210000003169 central nervous system Anatomy 0.000 claims 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- 108091005461 Nucleic proteins Proteins 0.000 claims 2
- 229930040373 Paraformaldehyde Natural products 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- 238000005352 clarification Methods 0.000 claims 2
- 230000005684 electric field Effects 0.000 claims 2
- 239000002563 ionic surfactant Substances 0.000 claims 2
- 238000002372 labelling Methods 0.000 claims 2
- 238000007431 microscopic evaluation Methods 0.000 claims 2
- 238000000386 microscopy Methods 0.000 claims 2
- 210000000653 nervous system Anatomy 0.000 claims 2
- 230000003287 optical effect Effects 0.000 claims 2
- 229920002866 paraformaldehyde Polymers 0.000 claims 2
- 230000001575 pathological effect Effects 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims 1
- 208000024172 Cardiovascular disease Diseases 0.000 claims 1
- 208000017701 Endocrine disease Diseases 0.000 claims 1
- 208000018522 Gastrointestinal disease Diseases 0.000 claims 1
- 208000022555 Genital disease Diseases 0.000 claims 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims 1
- 208000019693 Lung disease Diseases 0.000 claims 1
- 208000023178 Musculoskeletal disease Diseases 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 108010026552 Proteome Proteins 0.000 claims 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 1
- 229940072056 alginate Drugs 0.000 claims 1
- 238000011888 autopsy Methods 0.000 claims 1
- 238000001574 biopsy Methods 0.000 claims 1
- 210000004556 brain Anatomy 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 201000011510 cancer Diseases 0.000 claims 1
- 238000004132 cross linking Methods 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 claims 1
- 208000010643 digestive system disease Diseases 0.000 claims 1
- 201000010099 disease Diseases 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 1
- 238000007877 drug screening Methods 0.000 claims 1
- 230000004064 dysfunction Effects 0.000 claims 1
- 230000002526 effect on cardiovascular system Effects 0.000 claims 1
- 238000001493 electron microscopy Methods 0.000 claims 1
- 230000002124 endocrine Effects 0.000 claims 1
- 208000030172 endocrine system disease Diseases 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 238000007306 functionalization reaction Methods 0.000 claims 1
- 208000018685 gastrointestinal system disease Diseases 0.000 claims 1
- 230000003100 immobilizing effect Effects 0.000 claims 1
- 210000000987 immune system Anatomy 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 238000013507 mapping Methods 0.000 claims 1
- 238000002705 metabolomic analysis Methods 0.000 claims 1
- 230000001431 metabolomic effect Effects 0.000 claims 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical group O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims 1
- 238000004452 microanalysis Methods 0.000 claims 1
- 208000017445 musculoskeletal system disease Diseases 0.000 claims 1
- 238000000399 optical microscopy Methods 0.000 claims 1
- 210000000056 organ Anatomy 0.000 claims 1
- 238000012545 processing Methods 0.000 claims 1
- 238000004393 prognosis Methods 0.000 claims 1
- 230000005855 radiation Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 claims 1
- 238000004621 scanning probe microscopy Methods 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 230000004855 vascular circulation Effects 0.000 claims 1
- OZAIFHULBGXAKX-UHFFFAOYSA-N 2-(2-cyanopropan-2-yldiazenyl)-2-methylpropanenitrile Chemical compound N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 description 4
- 229920000954 Polyglycolide Polymers 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 239000004633 polyglycolic acid Substances 0.000 description 2
- 239000004626 polylactic acid Substances 0.000 description 2
- 239000004971 Cross linker Substances 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- YXHKONLOYHBTNS-UHFFFAOYSA-N Diazomethane Chemical compound C=[N+]=[N-] YXHKONLOYHBTNS-UHFFFAOYSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 229920001400 block copolymer Polymers 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920001606 poly(lactic acid-co-glycolic acid) Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 150000003673 urethanes Chemical class 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261681551P | 2012-08-09 | 2012-08-09 | |
| US61/681,551 | 2012-08-09 | ||
| PCT/US2013/031066 WO2014025392A1 (en) | 2012-08-09 | 2013-03-13 | Methods and compositions for preparing biological specimens for microscopic analysis |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015533210A JP2015533210A (ja) | 2015-11-19 |
| JP2015533210A5 true JP2015533210A5 (cg-RX-API-DMAC7.html) | 2017-05-25 |
| JP6228205B2 JP6228205B2 (ja) | 2017-11-08 |
Family
ID=50068460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015526521A Expired - Fee Related JP6228205B2 (ja) | 2012-08-09 | 2013-03-13 | 顕微分析のための生体標本を調製するための方法および組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10545075B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP2883030B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6228205B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR102148747B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN104350372B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2013300171B2 (cg-RX-API-DMAC7.html) |
| IN (1) | IN2014DN09073A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2014025392A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (167)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| CA2850509C (en) | 2011-10-14 | 2023-08-01 | President And Fellows Of Harvard College | Sequencing by structure assembly |
| US10227639B2 (en) | 2011-12-22 | 2019-03-12 | President And Fellows Of Harvard College | Compositions and methods for analyte detection |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
| IN2014DN09073A (cg-RX-API-DMAC7.html) | 2012-08-09 | 2015-05-22 | Univ Leland Stanford Junior | |
| EP2909337B1 (en) | 2012-10-17 | 2019-01-09 | Spatial Transcriptomics AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| EP2971184B1 (en) | 2013-03-12 | 2019-04-17 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| DE102013205115A1 (de) * | 2013-03-22 | 2014-09-25 | Leica Microsystems Cms Gmbh | SPIM-Anordnung |
| WO2014210223A1 (en) | 2013-06-25 | 2014-12-31 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| DE102013216934A1 (de) * | 2013-08-26 | 2015-02-26 | Leica Microsystems Cms Gmbh | Verfahren und Vorrichtung zur Erhöhung der optischen Transparenz von Bereichen einer Gewebeprobe |
| EP3047271B1 (en) * | 2013-09-20 | 2022-05-11 | California Institute Of Technology | Methods for phenotyping of intact whole tissues |
| US10794802B2 (en) * | 2013-09-20 | 2020-10-06 | California Institute Of Technology | Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping |
| CN103558055B (zh) * | 2013-09-29 | 2016-01-06 | 中国疾病预防控制中心病毒病预防控制所 | 一种针对感染病毒的病变细胞的定位超薄切片方法 |
| WO2015127183A2 (en) * | 2014-02-21 | 2015-08-27 | Massachusetts Institute Of Technology | Expansion microscopy |
| CN106211762B (zh) | 2014-04-04 | 2020-08-14 | 麻省理工学院 | 向带电基质中、在带电基质中和/或从带电基质中主动转运带电分子 |
| CN106164736A (zh) * | 2014-05-30 | 2016-11-23 | 斯坦福大学托管董事会 | 用于对大的完整组织样品进行成像的方法及装置 |
| EP3168290B1 (en) * | 2014-07-07 | 2021-12-08 | Logos Biosystems, Inc. | Apparatus for clearing tissue using electrophoresis |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| ES2968004T3 (es) | 2014-07-30 | 2024-05-06 | Harvard College | Construcción de bibliotecas de sondas |
| WO2016023009A1 (en) * | 2014-08-07 | 2016-02-11 | Wake Forest University Health Sciences | Compositions and methods for clearing a biological sample |
| KR101563826B1 (ko) * | 2014-12-31 | 2015-10-28 | 계명대학교 산학협력단 | 조직 투명화 방법 |
| AU2016218055B2 (en) | 2015-02-09 | 2019-05-16 | Ventana Medical Systems, Inc. | Materials and methods for standardizing diffusion of a fluid into tissues |
| ES2955488T3 (es) | 2015-04-10 | 2023-12-01 | Spatial Transcriptomics Ab | Análisis multiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos |
| US10059990B2 (en) | 2015-04-14 | 2018-08-28 | Massachusetts Institute Of Technology | In situ nucleic acid sequencing of expanded biological samples |
| US11408890B2 (en) | 2015-04-14 | 2022-08-09 | Massachusetts Institute Of Technology | Iterative expansion microscopy |
| US10526649B2 (en) | 2015-04-14 | 2020-01-07 | Massachusetts Institute Of Technology | Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information |
| CN106198990A (zh) * | 2015-04-30 | 2016-12-07 | 上海交通大学 | 一种对组织样本进行免疫标记的方法 |
| CN104965016A (zh) * | 2015-06-15 | 2015-10-07 | 南昌大学 | 一体化的生物组织透明装置 |
| US10364457B2 (en) | 2015-08-07 | 2019-07-30 | Massachusetts Institute Of Technology | Nanoscale imaging of proteins and nucleic acids via expansion microscopy |
| CA2994957A1 (en) | 2015-08-07 | 2017-02-16 | Massachusetts Institute Of Technology | Protein retention expansion microscopy |
| WO2017031249A1 (en) * | 2015-08-17 | 2017-02-23 | California Institute Of Technology | Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping |
| WO2017075265A1 (en) * | 2015-10-28 | 2017-05-04 | The Broad Institute, Inc. | Multiplex analysis of single cell constituents |
| MX2018005611A (es) | 2015-11-03 | 2018-11-09 | Harvard College | Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico. |
| KR20180091024A (ko) * | 2015-12-02 | 2018-08-14 | 클리어라이트 다이어그노스틱스 엘엘씨 | 암의 검출 및 모니터링을 위해 종양 조직 시료를 준비하고 분석하는 방법 |
| CN108431664B (zh) | 2015-12-23 | 2021-11-02 | 皇家飞利浦有限公司 | 荧光校准载片 |
| CN105823807B (zh) * | 2016-01-04 | 2018-04-20 | 浙江农林大学 | 抗癌药物羟喜树碱药效检测装置及检测方法 |
| US11254974B2 (en) | 2016-02-10 | 2022-02-22 | The Board Of Trustees Of The Leland Stanford Junior University | RNA fixation and detection in clarity-based hydrogel tissue |
| JP7038062B2 (ja) * | 2016-03-07 | 2022-03-17 | エックス－ゼル インコーポレイテッド | 希少細胞を同定する組成物及び方法 |
| JP6721899B2 (ja) * | 2016-03-30 | 2020-07-15 | 国立大学法人 大分大学 | 高親水性高分子モノマーを主剤とする電子顕微鏡用包埋方法 |
| CA3022290A1 (en) | 2016-04-25 | 2017-11-02 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| US11397140B2 (en) * | 2016-04-29 | 2022-07-26 | Massachusetts Institute Of Technology | Methods for reversible and tunable tissue magnification |
| US10324022B2 (en) | 2016-05-11 | 2019-06-18 | Bonraybio Co., Ltd. | Analysis accuracy improvement in automated testing apparatus |
| US10281386B2 (en) * | 2016-05-11 | 2019-05-07 | Bonraybio Co., Ltd. | Automated testing apparatus |
| US10852290B2 (en) | 2016-05-11 | 2020-12-01 | Bonraybio Co., Ltd. | Analysis accuracy improvement in automated testing apparatus |
| WO2017205531A1 (en) * | 2016-05-25 | 2017-11-30 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for visualization and quantification of fiber-like structures |
| US10495554B2 (en) * | 2016-05-25 | 2019-12-03 | The Board Of Trustees Of The Leland Stanford Junior University | Method and system for imaging and analysis of a biological specimen |
| US11352667B2 (en) | 2016-06-21 | 2022-06-07 | 10X Genomics, Inc. | Nucleic acid sequencing |
| CN107621462B (zh) * | 2016-07-13 | 2020-03-31 | 王志伟 | 一种组织透明化液sut及其制备和应用 |
| CN107674879A (zh) * | 2016-08-01 | 2018-02-09 | 深圳先进技术研究院 | 一种光‑基因质粒及其应用 |
| US11480502B2 (en) | 2016-08-29 | 2022-10-25 | United States of America, as represented by the Secretary, Department of Heatlth and Human Service | Method and composition for optical clearing of tissues |
| EP4582805A3 (en) | 2016-08-31 | 2025-10-08 | President And Fellows Of Harvard College | Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing |
| EP4428536A3 (en) | 2016-08-31 | 2024-12-04 | President and Fellows of Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
| US10538632B1 (en) | 2016-09-30 | 2020-01-21 | National Technology & Engineering Solutions Of Sandia, Llc | Shape-preserving polymeric replication of biological matter |
| JP6898720B2 (ja) * | 2016-10-14 | 2021-07-07 | 株式会社ファンケル | 皮膚内部構造評価方法 |
| US20190264270A1 (en) * | 2016-11-08 | 2019-08-29 | President And Fellows Of Harvard College | Matrix imprinting and clearing |
| EP4339956A3 (en) * | 2016-11-08 | 2024-06-19 | President and Fellows of Harvard College | Multiplexed imaging using merfish, expansion microscopy, and related technologies |
| KR101866249B1 (ko) | 2016-11-29 | 2018-06-12 | 박순현 | 생체 조직 투명화용 조성물 및 이를 이용한 생체 조직 투명화 방법 |
| DE102016123458B3 (de) * | 2016-12-05 | 2018-03-15 | Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin | Verfahren zur Herstellung transparenter biologischer Präparate für eine lichtmikroskopische Untersuchung |
| CN106682453B (zh) * | 2016-12-28 | 2019-03-26 | 长春理工大学 | 一种dna分子的定点编辑方法 |
| US10955322B2 (en) | 2017-01-18 | 2021-03-23 | California Institute Of Technology | Methods and devices for soft and osseous tissue clearing and fluorescent imaging |
| CN106769350B (zh) * | 2017-01-20 | 2019-11-22 | 上海交通大学 | 一种用于富含脂滴组织快速完全透明化的方法 |
| WO2018136856A1 (en) | 2017-01-23 | 2018-07-26 | Massachusetts Institute Of Technology | Multiplexed signal amplified fish via splinted ligation amplification and sequencing |
| KR101888782B1 (ko) * | 2017-02-07 | 2018-08-14 | 고려대학교 산학협력단 | 피부 조직의 투명화 방법, 이를 이용한 피부 조직의 3차원 영상화 방법 및 평가 및 진단 방법 |
| WO2018151223A1 (ja) * | 2017-02-16 | 2018-08-23 | 国立大学法人京都大学 | 細胞評価方法、細胞評価装置、及び細胞評価プログラム |
| WO2018157048A1 (en) | 2017-02-24 | 2018-08-30 | Massachusetts Institute Of Technology | Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy |
| WO2018157074A1 (en) | 2017-02-24 | 2018-08-30 | Massachusetts Institute Of Technology | Methods for diagnosing neoplastic lesions |
| WO2018155925A2 (ko) * | 2017-02-27 | 2018-08-30 | 고려대학교 산학협력단 | 하이드로겔 중합체를 이용한 탈세포화 조직의 제조방법 및 이로부터 제조된 탈세포화 조직 |
| KR20180099482A (ko) * | 2017-02-27 | 2018-09-05 | 고려대학교 산학협력단 | 하이드로겔 중합체를 이용한 탈세포화 조직의 제조방법 및 이로부터 제조된 탈세포화 조직 |
| TWI620927B (zh) * | 2017-04-21 | 2018-04-11 | 胡宇光 | 神經組織樣本染色之方法及套組與顯現神經元之方法 |
| US11788123B2 (en) | 2017-05-26 | 2023-10-17 | President And Fellows Of Harvard College | Systems and methods for high-throughput image-based screening |
| MX2017008919A (es) * | 2017-07-05 | 2018-03-01 | Gabriela Reyes Fuchs Carmen | Proceso para formar una imagen a color de materiales incinerados mediante tecnicas de microscopia. |
| US11719606B2 (en) | 2017-07-06 | 2023-08-08 | University Public Corporation Osaka | Method and reagent for clearing biological tissue |
| KR101996722B1 (ko) * | 2017-07-07 | 2019-07-04 | 재단법인대구경북과학기술원 | 다채널 박막전극을 이용한 전기적 생체분자 이동방법 |
| US11180804B2 (en) | 2017-07-25 | 2021-11-23 | Massachusetts Institute Of Technology | In situ ATAC sequencing |
| US11597965B2 (en) | 2017-10-06 | 2023-03-07 | 10X Genomics, Inc. | RNA templated ligation |
| WO2019078655A1 (ko) * | 2017-10-19 | 2019-04-25 | 재단법인대구경북과학기술원 | 설포베타인계 쯔비터이온성 계면활성제를 포함하는 생물학적 분자의 조직침투용 조성물 및 이의 용도 |
| KR102200618B1 (ko) * | 2018-01-12 | 2021-01-08 | 재단법인대구경북과학기술원 | 설포베타인계 쯔비터이온성 계면활성제를 포함하는 생물학적 분자의 조직침투용 조성물 및 이의 용도 |
| US10488307B2 (en) * | 2017-12-12 | 2019-11-26 | Dignity Health | Systems, devices, and methods for improved tissue treatment |
| KR102050248B1 (ko) * | 2017-12-29 | 2019-11-29 | 연세대학교 산학협력단 | 조직 투명도 분석 방법 |
| CN107966477A (zh) * | 2018-01-22 | 2018-04-27 | 哈尔滨工业大学 | 一种生物敏感元件 |
| US11873374B2 (en) | 2018-02-06 | 2024-01-16 | Massachusetts Institute Of Technology | Swellable and structurally homogenous hydrogels and methods of use thereof |
| GB2571544B (en) | 2018-02-28 | 2022-05-18 | Bright Instrument Co Ltd | Sectioning macrotome |
| JP7424644B2 (ja) * | 2018-03-16 | 2024-01-30 | クライオカプセル | 光学及び／又は電子顕微鏡による生体試料の画像化のための生体試料の包埋液 |
| WO2020011735A2 (en) | 2018-07-09 | 2020-01-16 | Universität Zürich | Systems, apparatuses, and methods for preparation of tissue samples |
| WO2020013833A1 (en) | 2018-07-13 | 2020-01-16 | Massachusetts Institute Of Technology | Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm) |
| WO2020028194A1 (en) | 2018-07-30 | 2020-02-06 | Readcoor, Inc. | Methods and systems for sample processing or analysis |
| KR102151128B1 (ko) * | 2018-08-24 | 2020-09-02 | 주식회사 바이나리 | 질소 가스 가압식 조직 투명화 및 항체 침투 증진용 장치 |
| JP7246752B2 (ja) * | 2018-08-29 | 2023-03-28 | 国立研究開発法人物質・材料研究機構 | 測定装置、及び、評価方法 |
| KR102368585B1 (ko) * | 2018-09-28 | 2022-03-03 | 서울대학교산학협력단 | 온도에 따라 가역적 팽창/초기화가 가능한 하이드로젤을 이용한 생체조직 이미징 |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| US11435302B2 (en) * | 2018-11-13 | 2022-09-06 | The Trustees Of Princeton University | X-ray assisted electron microscopy staining procedure |
| GB201818567D0 (en) * | 2018-11-14 | 2018-12-26 | Francis Crick Institute Ltd | Composition |
| EP3894583A4 (en) | 2018-12-13 | 2022-11-09 | President and Fellows of Harvard College | AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS |
| CN109612811A (zh) * | 2018-12-26 | 2019-04-12 | 华中科技大学苏州脑空间信息研究院 | 一种保护生物组织结构和荧光的水凝胶包埋方法 |
| CN109781493A (zh) * | 2018-12-26 | 2019-05-21 | 华中科技大学苏州脑空间信息研究院 | 一种可长时间连续精细切削成像的生物组织水凝胶包埋方法 |
| CA3130889A1 (en) | 2019-02-22 | 2020-08-27 | Massachusetts Institute Of Technology | Iterative direct expansion microscopy |
| CN110006862B (zh) * | 2019-03-29 | 2020-09-08 | 华中科技大学 | 膨胀切削显微成像方法及适用于该方法的超吸水水凝胶 |
| KR102340869B1 (ko) * | 2019-05-09 | 2021-12-20 | (주)크레용테크놀러지즈 | 이온 전도성 필름을 이용한 전기 영동 방식의 생체 시료 염색 방법 및 염색 장치 |
| WO2020243564A1 (en) * | 2019-05-30 | 2020-12-03 | Beckman Coulter, Inc. | Methods and systems for immobilizing a biological specimen for microscopic imaging |
| CN113906147B (zh) | 2019-05-31 | 2024-09-13 | 10X基因组学有限公司 | 检测目标核酸分子的方法 |
| US12265004B2 (en) | 2019-11-05 | 2025-04-01 | Massachusetts Institute Of Technology | Membrane probes for expansion microscopy |
| JPWO2021095718A1 (cg-RX-API-DMAC7.html) * | 2019-11-11 | 2021-05-20 | ||
| WO2021113505A1 (en) | 2019-12-05 | 2021-06-10 | Massachusetts Institute Of Technology | Method for preparing a specimen for expansion microscopy |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| US12215379B2 (en) | 2020-02-17 | 2025-02-04 | 10X Genomics, Inc. | In situ analysis of chromatin interaction |
| EP4107285B1 (en) | 2020-02-21 | 2024-10-09 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| US20230069991A1 (en) * | 2020-02-28 | 2023-03-09 | Miltenyi Biotec B.V. & Co. KG | Method for extraction of target cells from 3D tissue by optical identification |
| US12188085B2 (en) | 2020-03-05 | 2025-01-07 | 10X Genomics, Inc. | Three-dimensional spatial transcriptomics with sequencing readout |
| CN115551638A (zh) * | 2020-03-13 | 2022-12-30 | 耶鲁大学 | 用于生物样品的物理膨胀和成像的方法和系统 |
| US20210348991A1 (en) * | 2020-05-05 | 2021-11-11 | Massachusetts Institute Of Technology | Compositions and methods relating to reversibly compressible tissue-hydrogel hybrids |
| CN111397999B (zh) * | 2020-05-09 | 2021-12-03 | 中国科学院植物研究所 | 一种增强显微ct植物样品对比度的简易方法 |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| CN114129780B (zh) * | 2020-09-03 | 2022-06-28 | 天津大学 | 一种光控释放一氧化氮的复合水凝胶的制备及应用 |
| RU2752751C1 (ru) * | 2020-09-28 | 2021-08-02 | Ахмеджан Атаханович Аллаберганов | Способ получения цифровых двойников документа в виде бумажного носителя текстовой информации или в виде изображения текстового документа |
| US20220121900A1 (en) * | 2020-10-19 | 2022-04-21 | Arizona Board Of Regents On Behalf Of Northern Arizona University | Methods and systems for generating unclonable optical tags |
| CN112284861B (zh) * | 2020-10-21 | 2023-02-17 | 上海市农业科学院 | 用于真姬菇担孢子显微观察的固定液、制备方法、固定方法及应用 |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| US11333588B1 (en) * | 2020-12-07 | 2022-05-17 | Nebulum Technologies Co., Ltd. | Matrix-assisted methods and compositions to prepare biological samples for super-resolution imaging |
| EP4012046A1 (en) | 2020-12-11 | 2022-06-15 | 10X Genomics, Inc. | Methods and compositions for multimodal in situ analysis |
| CN112665951B (zh) * | 2020-12-22 | 2022-11-11 | 中国农业科学院作物科学研究所 | 一种胚乳组织包埋方法与应用 |
| US20220195498A1 (en) | 2020-12-23 | 2022-06-23 | 10X Genomics, Inc. | Methods and compositions for analyte detection |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| EP4284945B1 (en) | 2021-01-26 | 2024-10-23 | 10X Genomics, Inc. | Nucleic acid analog probes for in situ analysis |
| KR102762387B1 (ko) * | 2021-02-24 | 2025-02-05 | 한국과학기술원 | 화학적 염색과 팽창 이미징 기법의 최적화 방법 |
| US12275984B2 (en) | 2021-03-02 | 2025-04-15 | 10X Genomics, Inc. | Sequential hybridization and quenching |
| WO2022187366A1 (en) | 2021-03-03 | 2022-09-09 | 10X Genomics, Inc. | Analyte detection in situ using nucleic acid origami |
| CN113004885B (zh) * | 2021-03-08 | 2024-06-11 | 光华临港工程应用技术研发(上海)有限公司 | 一种用于膨胀超分辨成像的dna纳米结构染料及其应用 |
| CN117396613A (zh) | 2021-06-01 | 2024-01-12 | 10X基因组学有限公司 | 用于分析物检测和探针解析的方法和组合物 |
| WO2022256422A1 (en) | 2021-06-02 | 2022-12-08 | 10X Genomics, Inc. | Sample analysis using asymmetric circularizable probes |
| EP4367262A1 (en) | 2021-07-09 | 2024-05-15 | 10X Genomics, Inc. | Methods for detecting analytes using sparse labelling |
| EP4370896A1 (en) | 2021-07-13 | 2024-05-22 | 10X Genomics, Inc. | Methods for preparing polymerized matrix with controllable thickness |
| WO2023009842A1 (en) | 2021-07-30 | 2023-02-02 | 10X Genomics, Inc. | Methods and compositions for synchronizing reactions in situ |
| US12139751B2 (en) | 2021-07-30 | 2024-11-12 | 10X Genomics, Inc. | Circularizable probes for in situ analysis |
| US12391984B2 (en) | 2021-08-03 | 2025-08-19 | 10X Genomics, Inc. | Compositions and methods for rolling circle amplification |
| WO2023015192A1 (en) | 2021-08-03 | 2023-02-09 | 10X Genomics, Inc. | Nucleic acid concatemers and methods for stabilizing and/or compacting the same |
| US12460251B2 (en) | 2021-08-03 | 2025-11-04 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid molecules |
| US12435364B2 (en) | 2021-08-16 | 2025-10-07 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| EP4423296A2 (en) | 2021-12-27 | 2024-09-04 | 10X Genomics, Inc. | Methods and compositions for rolling circle amplification |
| US12497653B2 (en) | 2022-01-21 | 2025-12-16 | 10X Genomics, Inc. | Multiple readout signals for analyzing a sample |
| WO2023172915A1 (en) | 2022-03-08 | 2023-09-14 | 10X Genomics, Inc. | In situ code design methods for minimizing optical crowding |
| AU2023243205A1 (en) | 2022-04-01 | 2024-09-19 | 10X Genomics, Inc. | Compositions and methods for targeted masking of autofluorescence |
| EP4504969A1 (en) | 2022-04-06 | 2025-02-12 | 10X Genomics, Inc. | Methods for multiplex cell analysis |
| WO2023204581A1 (ko) * | 2022-04-20 | 2023-10-26 | 동국대학교 산학협력단 | 암세포 사멸효과를 가지는 3d 하이드로젤 복합체 및 이의 제조방법 |
| WO2023215612A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Analysis of antigen and antigen receptor interactions |
| WO2023215603A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of v(d)j sequences |
| JP2025516589A (ja) | 2022-05-11 | 2025-05-30 | １０エックス ジェノミクス インコーポレイテッド | インサイチュー配列決定のための組成物及び方法 |
| EP4540607A1 (en) | 2022-06-17 | 2025-04-23 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
| KR102582110B1 (ko) * | 2022-07-18 | 2023-09-21 | (재)씨젠의료재단 | 3차원 세포 또는 생체 조직의 투명화 및 이미지 분석용 조성물 |
| EP4511510B1 (en) | 2022-08-16 | 2025-12-10 | 10X Genomics, Inc. | Ap50 polymerases and uses thereof |
| KR20240042887A (ko) * | 2022-09-26 | 2024-04-02 | 연세대학교 산학협력단 | 고배율의 세포 확대 분석을 위한 세포 필름, 이의 제조용 조성물 및 제조 방법 |
| CN115656120B (zh) * | 2022-10-09 | 2024-07-23 | 中国科学院深圳先进技术研究院 | 一种暗场反射紫外光学显微成像方法和系统 |
| EP4602344A1 (en) | 2022-10-14 | 2025-08-20 | 10X Genomics, Inc. | Methods for analysis of biological samples |
| EP4573210A1 (en) | 2022-11-16 | 2025-06-25 | 10X Genomics, Inc. | Methods and compositions for assessing performance of in situ assays |
| EP4634404A1 (en) | 2022-12-16 | 2025-10-22 | 10X Genomics, Inc. | Methods and compositions for assessing performance |
| WO2024137860A1 (en) | 2022-12-21 | 2024-06-27 | 10X Genomics, Inc. | Methods for tethering ribonucleic acids in biological samples |
| CN121014083A (zh) * | 2023-03-31 | 2025-11-25 | 富士胶片株式会社 | 药物研发辅助装置、药物研发辅助装置的工作方法、及药物研发辅助装置的工作程序 |
| EP4461816A1 (en) | 2023-05-10 | 2024-11-13 | 10X Genomics, Inc. | Boronic acid compositions and methods for tethering ribonucleic acids in biological samples |
| TWI887661B (zh) * | 2023-05-25 | 2025-06-21 | 國立清華大學 | 用於將生物組織透明化的電極組及裝置 |
| WO2025029831A1 (en) | 2023-07-31 | 2025-02-06 | 10X Genomics, Inc. | Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification |
| WO2025111463A1 (en) | 2023-11-22 | 2025-05-30 | 10X Genomics, Inc. | Methods for processing ribonucleic acids in biological samples |
| EP4578960A1 (en) | 2023-12-20 | 2025-07-02 | 10x Genomics, Inc. | In situ detection of copy number variations in biological samples |
| WO2025255457A1 (en) | 2024-06-07 | 2025-12-11 | 10X Genomics, Inc. | Compositions and methods for improved on-target spatial profiling |
Family Cites Families (87)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3129158A (en) | 1961-01-24 | 1964-04-14 | Raymond Samuel | Process for gel electrophoresis |
| US3208929A (en) | 1962-09-11 | 1965-09-28 | Robert H Raymond | Apparatus for gel electrophoresis |
| US3346479A (en) | 1964-04-09 | 1967-10-10 | Scientific Industries | Preparative separation by a combination of gel separation and electrophoresis |
| US3375187A (en) | 1965-05-13 | 1968-03-26 | Buchler Instr Inc | Apparatus for temperature controlled preparative and analytical electrophoresis |
| US3576727A (en) | 1968-05-23 | 1971-04-27 | Bruce Lee Evatt | Gel electrophoresis process |
| US3563880A (en) | 1968-08-28 | 1971-02-16 | Arthur W Anderson | Gel electrophoresis unit |
| SE328135B (cg-RX-API-DMAC7.html) | 1968-09-19 | 1970-09-07 | Lkb Produkter Ab | |
| US3616454A (en) | 1969-03-13 | 1971-10-26 | Univ New York | Method of and apparatus for electrophoretic separation in a gel column |
| US3674678A (en) | 1970-10-28 | 1972-07-04 | Millipore Corp | Electrophoretic apparatus |
| US3865712A (en) | 1972-04-13 | 1975-02-11 | Lkb Produkter Ab | Electrode arrangement for an electrophoretical separation performed in a gel plate |
| US3989613A (en) | 1973-05-16 | 1976-11-02 | The Dow Chemical Company | Continuous balanced flow fixed boundary electrophoresis |
| US4088561A (en) | 1977-06-27 | 1978-05-09 | The United States Of America As Represented By The United States Department Of Energy | Apparatus for electrophoresis separation |
| US4151065A (en) | 1978-01-30 | 1979-04-24 | The Regents Of The University Of California | Horizontal slab gel electrophoresis |
| US4292161A (en) | 1979-01-03 | 1981-09-29 | Hoefer Scientific Instruments | Vertical gel slab electrophoresis apparatus |
| FR2496889A1 (fr) | 1980-12-22 | 1982-06-25 | Hours Michel | Cuve de migration et de reaction utilisable pour des analyses par electrophorese |
| US4339327A (en) | 1981-04-23 | 1982-07-13 | Tyler Jonathan M | Miniature slab gel electrophoresis system |
| US4375401A (en) | 1981-05-20 | 1983-03-01 | Nicholas Catsimpoolas | Electrophoresis system |
| DE3374082D1 (en) | 1982-07-23 | 1987-11-19 | Matthias Hediger | Apparatus for preparative gel electrophoresis, in particular for separating mixtures on a gradient gel polyacryl amide column |
| US4588491A (en) | 1984-02-27 | 1986-05-13 | International Biotechnologies, Inc. | Horizontal gel electrophoresis device |
| DE3580418D1 (de) * | 1984-07-31 | 1990-12-13 | Hitachi Ltd | Elektrophoretisches trennverfahren mit freier stroemung und apparat dafuer. |
| US4685025A (en) | 1985-03-14 | 1987-08-04 | Raychem Corporation | Conductive polymer circuit protection devices having improved electrodes |
| US5188963A (en) | 1989-11-17 | 1993-02-23 | Gene Tec Corporation | Device for processing biological specimens for analysis of nucleic acids |
| JPH03167472A (ja) * | 1989-11-27 | 1991-07-19 | Sumitomo Chem Co Ltd | 実験動物の胎児骨格染色法 |
| JP3216907B2 (ja) | 1992-05-18 | 2001-10-09 | 株式会社日立製作所 | 電子ズーム装置及び撮像装置 |
| JP3049537B2 (ja) * | 1995-02-03 | 2000-06-05 | 悌二 竹崎 | 生検試料の定着支持方法とその定着支持剤及び包埋カセット |
| AU2144899A (en) | 1998-01-13 | 1999-08-02 | Claude Gravel | Viral vectors expressing self-polymerizing neuronal intermediate filaments and their use |
| US6722395B2 (en) | 1998-01-13 | 2004-04-20 | James W. Overbeck | Depositing fluid specimens on substrates, resulting ordered arrays, techniques for analysis of deposited arrays |
| WO2000017355A2 (en) | 1998-09-18 | 2000-03-30 | Incyte Pharmaceuticals, Inc. | Human cytoskeleton associated proteins |
| US6232092B1 (en) | 1998-10-02 | 2001-05-15 | Rogers Imaging Corporation | Method for preparing biological specimens for visual analysis |
| US6219575B1 (en) | 1998-10-23 | 2001-04-17 | Babak Nemati | Method and apparatus to enhance optical transparency of biological tissues |
| US6409774B1 (en) * | 1999-06-11 | 2002-06-25 | Resolution Sciences Corporation | Electrophoresis-assisted staining of materials |
| WO2002037938A2 (en) | 2000-10-24 | 2002-05-16 | Oncosis Llc | Method and device for selectively targeting cells within a three -dimensional specimen |
| AUPR378001A0 (en) * | 2001-03-16 | 2001-04-12 | Proteome Systems Ltd | Protein chip |
| US6472216B1 (en) | 2001-07-24 | 2002-10-29 | Ann-Shyn Chiang | Aqueous tissue clearing solution |
| CN1257752C (zh) * | 2001-09-28 | 2006-05-31 | 生物药开发有限公司 | 多功能生物相容性水凝胶及其制备方法 |
| DE10209609A1 (de) | 2002-03-05 | 2003-10-02 | Europ Lab Molekularbiolog | Verfahren und Vorrichtung zur Parallelanalyse von Bio-Molekülen |
| US20050256588A1 (en) | 2002-06-28 | 2005-11-17 | Cardio, Inc. | Decellularized tissue |
| WO2004034890A2 (en) | 2002-10-17 | 2004-04-29 | Vacanti, Joseph, P. | Biological scaffolding material |
| JP4361057B2 (ja) * | 2003-04-30 | 2009-11-11 | モイル，ウィリアム，アール | 生体分子検出および細胞分類用センサ |
| US7713923B2 (en) | 2003-06-25 | 2010-05-11 | Massachusetts Institute Of Technology | Self-assembling peptides incorporating modifications and methods of use thereof |
| US8226715B2 (en) | 2003-06-30 | 2012-07-24 | Depuy Mitek, Inc. | Scaffold for connective tissue repair |
| US7326571B2 (en) | 2003-07-17 | 2008-02-05 | Boston Scientific Scimed, Inc. | Decellularized bone marrow extracellular matrix |
| US7660620B2 (en) | 2003-09-29 | 2010-02-09 | Medtronic, Inc. | Timing techniques for magnetic resonance imaging |
| US7955788B2 (en) * | 2003-10-30 | 2011-06-07 | Medtronic, Inc. | Bioprosthetic tissue preparation with synthetic hydrogels |
| WO2005062938A2 (en) | 2003-12-23 | 2005-07-14 | The Regents Of The University Of California | Chemical agents and methods for optical clearing of skin and mucous membranes to improve light-based diagnostics and therapeutics |
| US7632641B2 (en) | 2004-03-25 | 2009-12-15 | California Institute Of Technology | Hybridization chain reaction |
| US7452491B2 (en) * | 2004-05-13 | 2008-11-18 | Chung Yuan Christian University | Method for forming scaffolds |
| GB0414825D0 (en) * | 2004-07-02 | 2004-08-04 | Biostatus Ltd | Gel formulations and uses thereof |
| CN1256579C (zh) * | 2004-09-23 | 2006-05-17 | 上海交通大学 | 基于原子力显微镜观察的生物样品包埋块的制备方法 |
| US7727721B2 (en) | 2005-03-08 | 2010-06-01 | California Institute Of Technology | Hybridization chain reaction amplification for in situ imaging |
| WO2007030012A2 (en) * | 2005-09-05 | 2007-03-15 | Stichting Voor De Technische Wetenscahappen | Extracellular matrix imaging |
| US8163565B2 (en) * | 2005-12-08 | 2012-04-24 | Cytyc Corporation | Light curing fixative |
| EP1801573A1 (en) * | 2005-12-21 | 2007-06-27 | Boehringer Mannheim Gmbh | Method and apparatus for parallel two-dimensional electrophoresis |
| CA2636912A1 (en) * | 2006-01-13 | 2007-07-26 | Ventana Medical Systems, Inc. | Biological sample processing composition and method |
| AU2008288260B2 (en) | 2007-08-14 | 2014-10-02 | Smith & Nephew Plc | Scaffolds |
| DE102007045897A1 (de) | 2007-09-26 | 2009-04-09 | Carl Zeiss Microimaging Gmbh | Verfahren zur mikroskopischen dreidimensionalen Abbildung einer Probe |
| US9134521B2 (en) | 2008-07-30 | 2015-09-15 | The Regents Of The University Of California | Multidirectional selective plane illumination microscopy |
| US20100055733A1 (en) | 2008-09-04 | 2010-03-04 | Lutolf Matthias P | Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels |
| WO2010030358A1 (en) | 2008-09-12 | 2010-03-18 | Quickmbed, Inc. | Scaffold for tissue sample orientation |
| JP5721639B2 (ja) * | 2009-02-19 | 2015-05-20 | ダコ・デンマーク・エー／エス | 分子標的の検出のための方法と化合物 |
| CN102362179A (zh) | 2009-03-25 | 2012-02-22 | 洛斯阿拉莫斯国家安全有限责任公司 | 用于荧光光谱测定的光纤光学组件 |
| EP2425216B1 (en) * | 2009-04-27 | 2019-10-23 | Expedeon Holdings Ltd. | Programmable electrophoretic notch filter systems and methods |
| JP5858435B2 (ja) | 2010-03-12 | 2016-02-10 | 国立研究開発法人理化学研究所 | 生物材料用透明化試薬、及びその利用 |
| US8852614B2 (en) | 2010-03-12 | 2014-10-07 | The Board Of Trustees Of The Leland Stanford Junior University | Hydrogels with network defects enhanced by nanoparticle incorporation |
| US8867803B2 (en) | 2010-04-20 | 2014-10-21 | Eric J. Seibel | Optical projection tomography microscopy (OPTM) for large specimen sizes |
| US8927624B2 (en) | 2010-05-14 | 2015-01-06 | Fujifilm Imaging Colorants Limited | Dispersion, process for preparing a dispersion and ink jet printing ink |
| TWI425201B (zh) | 2010-10-01 | 2014-02-01 | Nat Univ Tsing Hua | 觀察厚組織三維結構之方法 |
| CN102072836B (zh) * | 2010-11-12 | 2014-02-19 | 深圳华大基因科技有限公司 | 生物大分子浓缩除盐方法及试剂盒 |
| WO2012103343A1 (en) | 2011-01-28 | 2012-08-02 | The Board Of Trustees Of The Leland Stanford Junior University | Functional targeted brain endoskeletonization |
| EP2703801B1 (en) | 2011-04-28 | 2020-11-25 | Riken | Method for making biological material transparent |
| EP2711682B1 (en) | 2011-05-20 | 2019-07-10 | Riken | Clarifying reagent for biological materials and use thereof |
| US9359636B2 (en) | 2011-07-27 | 2016-06-07 | The Rockefeller University | Methods for fixing and detecting RNA |
| JP5967528B2 (ja) | 2012-06-22 | 2016-08-10 | 国立研究開発法人理化学研究所 | 生物材料を透明化する方法および生物材料用透明化処理キット |
| DE102012211462A1 (de) | 2012-07-03 | 2014-01-23 | Carl Zeiss Microscopy Gmbh | Verfahren zur Vorbereitung und Durchführung der Aufnahme von Bildstapeln einer Probe aus verschiedenen Orientierungswinkeln |
| IN2014DN09073A (cg-RX-API-DMAC7.html) | 2012-08-09 | 2015-05-22 | Univ Leland Stanford Junior | |
| DE102012020240A1 (de) | 2012-10-12 | 2014-04-17 | Carl Zeiss Microscopy Gmbh | Mikroskop und Verfahren zur SPIM Mikroskopie |
| WO2014182528A2 (en) | 2013-04-30 | 2014-11-13 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding |
| EP3035026A4 (en) | 2013-08-14 | 2017-04-26 | Riken | Composition for preparing biomaterial with excellent light-transmitting property, and use thereof |
| DE102013216934A1 (de) | 2013-08-26 | 2015-02-26 | Leica Microsystems Cms Gmbh | Verfahren und Vorrichtung zur Erhöhung der optischen Transparenz von Bereichen einer Gewebeprobe |
| EP3047271B1 (en) | 2013-09-20 | 2022-05-11 | California Institute Of Technology | Methods for phenotyping of intact whole tissues |
| CN103513411B (zh) | 2013-09-27 | 2016-02-03 | 香港应用科技研究院有限公司 | 荧光显微镜中聚焦的装置和方法 |
| CN106164736A (zh) | 2014-05-30 | 2016-11-23 | 斯坦福大学托管董事会 | 用于对大的完整组织样品进行成像的方法及装置 |
| WO2016023009A1 (en) | 2014-08-07 | 2016-02-11 | Wake Forest University Health Sciences | Compositions and methods for clearing a biological sample |
| US20160131560A1 (en) | 2014-11-07 | 2016-05-12 | California Institute Of Technology | Methods for phenotyping of intact bones by tissue clearing and staining |
| WO2016117614A1 (ja) | 2015-01-20 | 2016-07-28 | 国立研究開発法人理化学研究所 | 生物材料用透明化試薬、システム及びその利用 |
| EP3273218A4 (en) | 2015-03-18 | 2018-12-26 | Riken | Method for observing biological material and clearing method |
| KR20180091024A (ko) | 2015-12-02 | 2018-08-14 | 클리어라이트 다이어그노스틱스 엘엘씨 | 암의 검출 및 모니터링을 위해 종양 조직 시료를 준비하고 분석하는 방법 |
-
2013
- 2013-03-13 IN IN9073DEN2014 patent/IN2014DN09073A/en unknown
- 2013-03-13 CN CN201380028711.3A patent/CN104350372B/zh not_active Expired - Fee Related
- 2013-03-13 WO PCT/US2013/031066 patent/WO2014025392A1/en not_active Ceased
- 2013-03-13 EP EP13828542.4A patent/EP2883030B1/en active Active
- 2013-03-13 KR KR1020147033139A patent/KR102148747B1/ko not_active Expired - Fee Related
- 2013-03-13 US US14/403,050 patent/US10545075B2/en active Active
- 2013-03-13 EP EP22192548.0A patent/EP4163617A1/en not_active Withdrawn
- 2013-03-13 AU AU2013300171A patent/AU2013300171B2/en not_active Ceased
- 2013-03-13 JP JP2015526521A patent/JP6228205B2/ja not_active Expired - Fee Related
-
2017
- 2017-02-13 US US15/431,388 patent/US20170219465A1/en not_active Abandoned
-
2020
- 2020-11-17 US US16/950,640 patent/US20210215581A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015533210A5 (cg-RX-API-DMAC7.html) | ||
| Tayebi et al. | Exosome purification and analysis using a facile microfluidic hydrodynamic trapping device | |
| Qin et al. | Bone marrow stromal/stem cell-derived extracellular vesicles regulate osteoblast activity and differentiation in vitro and promote bone regeneration in vivo | |
| CN104350372B (zh) | 用于制备供显微镜分析的生物样本的方法和组合物 | |
| Cruz et al. | Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease | |
| Shintaku et al. | On-chip separation and analysis of RNA and DNA from single cells | |
| US12042791B2 (en) | Method of osteogenic differentiation in microfluidic tissue culture systems | |
| CN105556309A (zh) | 用于完整全组织的表型分析的方法 | |
| EP3550029B1 (en) | Composition for biotissue clearing and biotissue clearing method using same | |
| US11802822B2 (en) | Multiplexed expansion (MultiExM) pathology | |
| AU2018330609A1 (en) | Systems, methods and hydrogels for cell culture and analysis | |
| JP2019507352A (ja) | 癌の検出およびモニタリングのための腫瘍組織サンプルの調製および分析のための方法 | |
| JP2018538531A (ja) | マイクロ流体デバイス、そのキット、及びその使用方法 | |
| Distler et al. | Mechanical properties of cell-and microgel bead-laden oxidized alginate-gelatin hydrogels | |
| CA3071316C (en) | Fixation and retention of extracellular vesicles | |
| WO2025101864A1 (en) | Methods, compositions, and kits for reducing mislocalization of analytes in spatial analysis assays | |
| Jaberi et al. | Engineering microgel packing to tailor the physical and biological properties of gelatin methacryloyl granular hydrogel scaffolds | |
| Kim et al. | Efficient CRISPR-Cas9-based knockdown of RUNX2 to induce chondrogenic differentiation of stem cells | |
| Ham et al. | Advanced enrichment and separation of extracellular vesicles through the super absorbent polymer nanosieves | |
| Caires et al. | Finding and tracing human MSC in 3D microenvironments with the photoconvertible protein Dendra2 | |
| US20250235872A1 (en) | Microplate carrier | |
| JP5190422B2 (ja) | 等電点電気泳動用膨潤ゲルの作成方法 | |
| JP7058275B2 (ja) | 電気泳動による精子の分離 | |
| JP6714259B2 (ja) | 測定装置及び測定方法 | |
| KR102827998B1 (ko) | 생체조직 투명화 조성물 및 이를 이용한 전기영동 투명화 방법 |