JP2013514073A5 - - Google Patents
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- Publication number
- JP2013514073A5 JP2013514073A5 JP2012543787A JP2012543787A JP2013514073A5 JP 2013514073 A5 JP2013514073 A5 JP 2013514073A5 JP 2012543787 A JP2012543787 A JP 2012543787A JP 2012543787 A JP2012543787 A JP 2012543787A JP 2013514073 A5 JP2013514073 A5 JP 2013514073A5
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- matrix
- arginine
- antibody fragment
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28805909P | 2009-12-18 | 2009-12-18 | |
| US61/288,059 | 2009-12-18 | ||
| PCT/EP2010/070076 WO2011073389A1 (en) | 2009-12-18 | 2010-12-17 | Wash solution and method for affinity chromatography |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016021798A Division JP2016128495A (ja) | 2009-12-18 | 2016-02-08 | 洗浄溶液及びアフィニティークロマトグラフィーの方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013514073A JP2013514073A (ja) | 2013-04-25 |
| JP2013514073A5 true JP2013514073A5 (enExample) | 2014-02-13 |
| JP5909450B2 JP5909450B2 (ja) | 2016-04-26 |
Family
ID=43598315
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012543787A Active JP5909450B2 (ja) | 2009-12-18 | 2010-12-17 | 洗浄溶液及びアフィニティークロマトグラフィーの方法 |
| JP2016021798A Pending JP2016128495A (ja) | 2009-12-18 | 2016-02-08 | 洗浄溶液及びアフィニティークロマトグラフィーの方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016021798A Pending JP2016128495A (ja) | 2009-12-18 | 2016-02-08 | 洗浄溶液及びアフィニティークロマトグラフィーの方法 |
Country Status (18)
| Country | Link |
|---|---|
| US (3) | US9505803B2 (enExample) |
| EP (2) | EP2513134B1 (enExample) |
| JP (2) | JP5909450B2 (enExample) |
| KR (1) | KR101858266B1 (enExample) |
| CN (1) | CN102725304B (enExample) |
| AU (1) | AU2010332778B2 (enExample) |
| BR (1) | BR112012014937B1 (enExample) |
| CA (1) | CA2784278C (enExample) |
| DK (2) | DK2513134T3 (enExample) |
| ES (2) | ES2651065T3 (enExample) |
| IL (1) | IL220164B (enExample) |
| LT (2) | LT2513134T (enExample) |
| MX (1) | MX2012007103A (enExample) |
| PL (2) | PL2513134T3 (enExample) |
| PT (2) | PT2513134T (enExample) |
| RU (1) | RU2564569C2 (enExample) |
| SG (2) | SG181771A1 (enExample) |
| WO (1) | WO2011073389A1 (enExample) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0716762A2 (pt) | 2006-09-13 | 2013-09-24 | Abbott Lab | melhorias da cultura celular |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| TWI610936B (zh) * | 2008-10-20 | 2018-01-11 | 艾伯維有限公司 | 使用蛋白質ａ親和性層析進行抗體之分離及純化 |
| TW201028433A (en) | 2008-10-20 | 2010-08-01 | Abbott Lab | Viral inactivation during purification of antibodies |
| CA2784278C (en) | 2009-12-18 | 2019-02-26 | Novartis Ag | Wash solution and method for affinity chromatography |
| CA2784959C (en) * | 2010-01-22 | 2018-06-26 | Boehringer Ingelheim International Gmbh | Chromatographic method for purifying fc-containing proteins |
| JP6366502B2 (ja) * | 2011-06-01 | 2018-08-01 | ノバルティス アーゲー | アフィニティクロマトグラフィーのための、洗浄溶液および方法 |
| CN104093744A (zh) | 2011-10-11 | 2014-10-08 | 弗·哈夫曼-拉罗切有限公司 | 双特异性抗体的改进的组装 |
| JP2016519145A (ja) * | 2013-05-15 | 2016-06-30 | メディミューン リミテッド | 組換え産生ポリペプチドの精製 |
| CN105848746B (zh) * | 2013-09-05 | 2019-07-09 | 豪夫迈·罗氏有限公司 | 用于层析重复利用的方法 |
| CN105407905B (zh) | 2014-01-03 | 2020-03-03 | 生物辐射实验室股份有限公司 | 蛋白a洗脱产物中杂质的去除 |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| SG10201908027QA (en) | 2015-03-13 | 2019-10-30 | Bristol Myers Squibb Co | Use of alkaline washes during chromatography to remove impurities |
| JP7084301B2 (ja) * | 2015-08-21 | 2022-06-14 | エフ．ホフマン－ラ ロシュ アーゲー | 低伝導率洗浄緩衝液を用いたアフィニティークロマトグラフィー精製方法 |
| WO2017032686A1 (en) | 2015-08-21 | 2017-03-02 | F. Hoffmann-La Roche Ag | Method for the reduction of host cell proteins in affinity chromatography |
| WO2017194593A1 (en) | 2016-05-11 | 2017-11-16 | Ge Healthcare Bioprocess R&D Ab | Method of cleaning and/or sanitizing a separation matrix |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US10513537B2 (en) | 2016-05-11 | 2019-12-24 | Ge Healthcare Bioprocess R&D Ab | Separation matrix |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| CN109311949B (zh) | 2016-05-11 | 2022-09-16 | 思拓凡生物工艺研发有限公司 | 储存分离基质的方法 |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| MX392628B (es) * | 2016-07-25 | 2025-03-24 | Cephalon Inc | Amortiguador de lavado de cromatografia de afinidad. |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| KR102578087B1 (ko) | 2017-08-17 | 2023-09-18 | 저스트-에보텍 바이오로직스, 아이엔씨. | 숙주세포 갈렉틴(galectins) 및 다른 오염물(contaminant)로부터 글리코실화 단백질을 정제하는 방법 |
| CN107793469B (zh) * | 2017-10-16 | 2021-07-30 | 上海药明生物技术有限公司 | 一种去除宿主细胞蛋白含量的亲和纯化工艺 |
| WO2019126554A1 (en) * | 2017-12-21 | 2019-06-27 | Genzyme Corporation | Methods for enhanced removal of impurities during protein a chromatography |
| WO2019183334A1 (en) | 2018-03-21 | 2019-09-26 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbents, devices and methods |
| MX2020010320A (es) | 2018-04-02 | 2021-01-08 | Amgen Inc | Composiciones de erenumab y usos de las mismas. |
| CN112105927B (zh) * | 2018-05-08 | 2024-06-25 | 沃特世科技公司 | 可用于pH梯度阳离子交换色谱法的方法、组合物和试剂盒 |
| PT3837273T (pt) * | 2018-08-14 | 2025-12-26 | Bristol Myers Squibb Co | Recuperação melhorada de proteínas |
| US20230399358A1 (en) * | 2019-10-08 | 2023-12-14 | North Carolina State University | Immunoglobulin purification peptides and their use |
| BR112022019076A2 (pt) * | 2020-03-27 | 2023-01-10 | HaemaLogiX Ltd | Composição e método |
| US20230143163A1 (en) * | 2020-04-27 | 2023-05-11 | Alexion Pharmaceuticals, Inc. | Protein a chromatography purification of an antibody |
| CN112827217A (zh) * | 2020-12-29 | 2021-05-25 | 苏州良辰生物医药科技有限公司 | 一种层析柱清洗液及其应用 |
| US20240383946A1 (en) * | 2022-12-12 | 2024-11-21 | H. Lundbeck A/S | Arginine wash in protein purification |
| WO2024233992A2 (en) * | 2023-05-11 | 2024-11-14 | Trophogen, Inc. | Methods of manufacturing highly glycosylated glycoprotein hormones and their analogs |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4704366A (en) | 1984-06-22 | 1987-11-03 | Bio-Rad Laboratories, Inc. | Process for binding IgG to protein A |
| US4857320A (en) * | 1987-02-19 | 1989-08-15 | Monsanto Company | Method of enhancing the solubility of tissue plasminogen activator |
| JPH01238534A (ja) * | 1988-03-17 | 1989-09-22 | Mitsui Toatsu Chem Inc | エンドトキシンの除去方法 |
| US5429746A (en) | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| DE69739673D1 (de) | 1996-11-27 | 2009-12-31 | Genentech Inc | Affinitätsreinigung von Polypeptid-Proteinen auf einer Protein A Matrix |
| ES2292682T3 (es) | 1998-05-06 | 2008-03-16 | Genentech, Inc. | Purificacion de anticuerpos mediante cromatografia de intercambio ionico. |
| ES2228052T3 (es) | 1998-06-01 | 2005-04-01 | Genentech, Inc. | Separacion demonomeros de anticuerpos de sus multimeros utilizando cromatografia de intercambio de iones. |
| EP3088412B1 (en) | 2001-03-09 | 2021-05-05 | Chugai Seiyaku Kabushiki Kaisha | Protein purification method |
| WO2002098531A1 (en) | 2001-06-05 | 2002-12-12 | Genetics Institute, Llc | Methods for purifying highly anionic proteins |
| ATE417858T1 (de) | 2002-02-05 | 2009-01-15 | Genentech Inc | Proteinaufreinigung |
| EP2261230B1 (en) | 2002-09-11 | 2017-05-10 | Chugai Seiyaku Kabushiki Kaisha | Protein purification method |
| MXPA05004290A (es) * | 2002-10-24 | 2005-11-23 | Enos Pharmaceuticals Inc | Formulaciones de l-arginina de liberacion sostenida, y metodos para su fabricacion y uso. |
| AU2003298783B2 (en) * | 2002-11-26 | 2010-11-04 | Abbvie Biotherapeutics Inc. | Chimeric and humanized antibodies to alpha5beta1 integrin that modulate angiogenesis |
| EP1599222B1 (en) * | 2003-01-08 | 2009-03-04 | Novartis Vaccines and Diagnostics, Inc. | Stabilized aqueous compositions comprising tissue factor pathway inhibitor (tfpi) or tissue factor pathway inhibitor variant |
| GB0304576D0 (en) | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
| EP1601697B1 (en) | 2003-02-28 | 2007-05-30 | Lonza Biologics plc | Antibody purification by Protein A and ion exchange chromatography |
| PT1678208E (pt) | 2003-10-27 | 2013-07-10 | Wyeth Llc | Remoção de agregados de elevado peso molecular utilizando cromatografia de hidroxiapatite |
| US8084032B2 (en) | 2004-01-21 | 2011-12-27 | Ajinomoto Co., Inc. | Purification method which prevents denaturation of an antibody |
| DE602006003108D1 (de) | 2005-03-03 | 2008-11-27 | Ajinomoto Kk | Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin |
| WO2006099308A2 (en) | 2005-03-11 | 2006-09-21 | Wyeth | A method of weak partitioning chromatography |
| CN102626610B (zh) | 2006-01-06 | 2014-10-01 | Emd密理博公司 | 亲和层析基质及其制备和使用方法 |
| US8263750B2 (en) | 2006-03-16 | 2012-09-11 | Amgen Inc. | Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step |
| US20100190210A1 (en) | 2006-03-20 | 2010-07-29 | Medarex, Inc. | Protein Purification |
| BRPI0712496A2 (pt) | 2006-06-02 | 2012-09-25 | Wyeth Corp | peptìdeo isolado, composição compreendendo o referido peptìdeo, ácido nucléico isolado e métodos de purificar uma proteìna na superfamìlia do fator beta de transformação do crescimento (tgf-?) e de estabilizar uma solução contendo uma proteìna na superfamìlia tgf-b. |
| US7754689B2 (en) * | 2006-06-02 | 2010-07-13 | Wyeth Llc | Finger-1 peptide analogs of the TGF-β superfamily |
| FI2061803T4 (fi) | 2006-08-28 | 2023-03-14 | Process for the purification of fc-containing proteins | |
| CL2007002615A1 (es) * | 2006-09-08 | 2008-04-18 | Wyeth Corp | Metodos para aislar o purificar un producto que comprende poner el producto enlazado en contacto con al menos una solucion de lavado que comprende arginina y luego eluir el producto; y dicho producto. |
| JP2010516651A (ja) | 2007-01-17 | 2010-05-20 | メルク セローノ ソシエテ アノニム | Ｆｃ含有タンパク質の精製のための方法 |
| RU2009148891A (ru) | 2007-06-01 | 2011-07-20 | Ф.Хоффманн-Ля Рош Аг (Ch) | Очистка иммуноглобулина |
| US20110105725A1 (en) | 2008-05-15 | 2011-05-05 | Novo Nordisk A/S | Antibody purification process |
| AU2009282234A1 (en) | 2008-08-14 | 2010-02-18 | Merck Sharp & Dohme Corp. | Methods for purifying antibodies using protein a affinity chromatography |
| CA2784278C (en) | 2009-12-18 | 2019-02-26 | Novartis Ag | Wash solution and method for affinity chromatography |
| JP6366502B2 (ja) * | 2011-06-01 | 2018-08-01 | ノバルティス アーゲー | アフィニティクロマトグラフィーのための、洗浄溶液および方法 |
-
2010
- 2010-12-17 CA CA2784278A patent/CA2784278C/en active Active
- 2010-12-17 PL PL10790452T patent/PL2513134T3/pl unknown
- 2010-12-17 PL PL17188657.5T patent/PL3272764T3/pl unknown
- 2010-12-17 LT LTEP10790452.6T patent/LT2513134T/lt unknown
- 2010-12-17 EP EP10790452.6A patent/EP2513134B1/en not_active Revoked
- 2010-12-17 DK DK10790452.6T patent/DK2513134T3/en active
- 2010-12-17 AU AU2010332778A patent/AU2010332778B2/en active Active
- 2010-12-17 PT PT107904526T patent/PT2513134T/pt unknown
- 2010-12-17 PT PT171886575T patent/PT3272764T/pt unknown
- 2010-12-17 DK DK17188657.5T patent/DK3272764T3/da active
- 2010-12-17 WO PCT/EP2010/070076 patent/WO2011073389A1/en not_active Ceased
- 2010-12-17 CN CN201080057457.6A patent/CN102725304B/zh active Active
- 2010-12-17 SG SG2012044707A patent/SG181771A1/en unknown
- 2010-12-17 KR KR1020127015524A patent/KR101858266B1/ko active Active
- 2010-12-17 ES ES10790452.6T patent/ES2651065T3/es active Active
- 2010-12-17 MX MX2012007103A patent/MX2012007103A/es unknown
- 2010-12-17 RU RU2012130151/10A patent/RU2564569C2/ru active
- 2010-12-17 LT LTEP17188657.5T patent/LT3272764T/lt unknown
- 2010-12-17 SG SG10201408384PA patent/SG10201408384PA/en unknown
- 2010-12-17 EP EP17188657.5A patent/EP3272764B1/en active Active
- 2010-12-17 JP JP2012543787A patent/JP5909450B2/ja active Active
- 2010-12-17 ES ES17188657T patent/ES2982297T3/es active Active
- 2010-12-17 US US13/516,960 patent/US9505803B2/en active Active
- 2010-12-17 BR BR112012014937-7A patent/BR112012014937B1/pt active IP Right Grant
-
2012
- 2012-06-04 IL IL220164A patent/IL220164B/en active IP Right Grant
-
2016
- 2016-02-08 JP JP2016021798A patent/JP2016128495A/ja active Pending
- 2016-10-28 US US15/336,878 patent/US20170044211A1/en not_active Abandoned
-
2019
- 2019-03-25 US US16/363,170 patent/US20190233469A1/en not_active Abandoned
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