JP2013514073A5 - - Google Patents
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- Publication number
- JP2013514073A5 JP2013514073A5 JP2012543787A JP2012543787A JP2013514073A5 JP 2013514073 A5 JP2013514073 A5 JP 2013514073A5 JP 2012543787 A JP2012543787 A JP 2012543787A JP 2012543787 A JP2012543787 A JP 2012543787A JP 2013514073 A5 JP2013514073 A5 JP 2013514073A5
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- matrix
- arginine
- antibody fragment
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28805909P | 2009-12-18 | 2009-12-18 | |
| US61/288,059 | 2009-12-18 | ||
| PCT/EP2010/070076 WO2011073389A1 (en) | 2009-12-18 | 2010-12-17 | Wash solution and method for affinity chromatography |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016021798A Division JP2016128495A (ja) | 2009-12-18 | 2016-02-08 | 洗浄溶液及びアフィニティークロマトグラフィーの方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013514073A JP2013514073A (ja) | 2013-04-25 |
| JP2013514073A5 true JP2013514073A5 (enExample) | 2014-02-13 |
| JP5909450B2 JP5909450B2 (ja) | 2016-04-26 |
Family
ID=43598315
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012543787A Active JP5909450B2 (ja) | 2009-12-18 | 2010-12-17 | 洗浄溶液及びアフィニティークロマトグラフィーの方法 |
| JP2016021798A Pending JP2016128495A (ja) | 2009-12-18 | 2016-02-08 | 洗浄溶液及びアフィニティークロマトグラフィーの方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016021798A Pending JP2016128495A (ja) | 2009-12-18 | 2016-02-08 | 洗浄溶液及びアフィニティークロマトグラフィーの方法 |
Country Status (18)
| Country | Link |
|---|---|
| US (3) | US9505803B2 (enExample) |
| EP (2) | EP3272764B1 (enExample) |
| JP (2) | JP5909450B2 (enExample) |
| KR (1) | KR101858266B1 (enExample) |
| CN (1) | CN102725304B (enExample) |
| AU (1) | AU2010332778B2 (enExample) |
| BR (1) | BR112012014937B1 (enExample) |
| CA (1) | CA2784278C (enExample) |
| DK (2) | DK3272764T3 (enExample) |
| ES (2) | ES2982297T3 (enExample) |
| IL (1) | IL220164B (enExample) |
| LT (2) | LT2513134T (enExample) |
| MX (1) | MX2012007103A (enExample) |
| PL (2) | PL2513134T3 (enExample) |
| PT (2) | PT2513134T (enExample) |
| RU (1) | RU2564569C2 (enExample) |
| SG (2) | SG10201408384PA (enExample) |
| WO (1) | WO2011073389A1 (enExample) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| TW201516149A (zh) | 2006-09-13 | 2015-05-01 | Abbvie Inc | 細胞培養改良 |
| CA2932207A1 (en) * | 2008-10-20 | 2010-12-09 | Abbvie Inc. | Isolation and purification of antibodies using protein a affinity chromatography |
| CN104974251A (zh) | 2008-10-20 | 2015-10-14 | Abbvie公司 | 在抗体纯化过程中的病毒灭活 |
| BR112012014937B1 (pt) | 2009-12-18 | 2021-01-19 | Novartis Ag | método de produção de um anticorpo, fragmento de anticorpo ou proteína de fusão fc purificada usando uma matriz de cromatografia de afinidade |
| EP2526114B1 (de) | 2010-01-22 | 2014-06-18 | Boehringer Ingelheim International GmbH | Chromatographisches verfahren zur aufreinigung von fc enthaltenden proteinen |
| KR101953405B1 (ko) * | 2011-06-01 | 2019-02-28 | 노파르티스 아게 | 친화성 크로마토그래피를 위한 세척 용액 및 방법 |
| SI2766397T1 (sl) | 2011-10-11 | 2018-09-28 | F. Hoffmann-La Roche Ag | Izboljšano sestavljanje bispecifičnih protiteles |
| US10259842B2 (en) * | 2013-05-15 | 2019-04-16 | Medimmune, Llc | Purification of recombinantly produced polypeptides |
| EP3041593B1 (en) * | 2013-09-05 | 2025-01-29 | F. Hoffmann-La Roche AG | Method of cleaning a chromatography material for its reuse |
| US9546208B2 (en) | 2014-01-03 | 2017-01-17 | Bio-Rad Laboratories, Inc. | Removal of impurities from protein A eluates |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| BR112017018365A2 (pt) | 2015-03-13 | 2018-04-10 | Bristol-Myers Squibb Company | uso de lavagens alcalinas durante cromatografia para remoção de impurezas |
| CN107849122B (zh) * | 2015-08-21 | 2022-01-25 | 豪夫迈·罗氏有限公司 | 用低电导率洗涤缓冲液进行亲和层析纯化 |
| MX388197B (es) | 2015-08-21 | 2025-03-19 | Hoffmann La Roche | Método para la reducción de proteínas de células hospederas en cromatografía de afinidad. |
| EP3455241B1 (en) | 2016-05-11 | 2022-02-23 | Cytiva BioProcess R&D AB | Method of cleaning and/or sanitizing a separation matrix |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| JP7122974B2 (ja) | 2016-05-11 | 2022-08-22 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 変異免疫グロブリン結合ポリペプチド |
| WO2017194592A1 (en) | 2016-05-11 | 2017-11-16 | Ge Healthcare Bioprocess R&D Ab | Method of storing a separation matrix |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| WO2018022628A1 (en) * | 2016-07-25 | 2018-02-01 | Cephalon, Inc. | Affinity chromatography wash buffer |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| MY199833A (en) | 2017-08-17 | 2023-11-24 | Just Evotec Biologics Inc | Method of purifying glycosylated protein from host cell galectins and other contaminants |
| CN107793469B (zh) * | 2017-10-16 | 2021-07-30 | 上海药明生物技术有限公司 | 一种去除宿主细胞蛋白含量的亲和纯化工艺 |
| AU2018392697B2 (en) * | 2017-12-21 | 2025-08-14 | Genzyme Corporation | Methods for enhanced removal of impurities during protein a chromatography |
| US11155575B2 (en) | 2018-03-21 | 2021-10-26 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbent, devices and methods |
| SG11202009629YA (en) | 2018-04-02 | 2020-10-29 | Amgen Inc | Erenumab compositions and uses thereof |
| CN112105927B (zh) * | 2018-05-08 | 2024-06-25 | 沃特世科技公司 | 可用于pH梯度阳离子交换色谱法的方法、组合物和试剂盒 |
| US12103949B2 (en) * | 2018-08-14 | 2024-10-01 | Bristol-Myers Squibb Company | Protein recovery |
| WO2021072005A1 (en) * | 2019-10-08 | 2021-04-15 | North Carolina State University | Immunoglobulin purification peptides and their use |
| JP7817945B2 (ja) * | 2020-03-27 | 2026-02-19 | ハエマロジックス・リミテッド | 組成物および方法 |
| US20230143163A1 (en) * | 2020-04-27 | 2023-05-11 | Alexion Pharmaceuticals, Inc. | Protein a chromatography purification of an antibody |
| CN112827217A (zh) * | 2020-12-29 | 2021-05-25 | 苏州良辰生物医药科技有限公司 | 一种层析柱清洗液及其应用 |
| EP4634201A1 (en) * | 2022-12-12 | 2025-10-22 | H. Lundbeck A/S | Arginine wash in protein purification |
| WO2024233992A2 (en) * | 2023-05-11 | 2024-11-14 | Trophogen, Inc. | Methods of manufacturing highly glycosylated glycoprotein hormones and their analogs |
| EP4729534A1 (en) | 2024-10-21 | 2026-04-22 | Technische Universität München, in Vertretung des Freistaats Bayern | Polypeptides comprising protein domains linked via rigid amino acid linkers |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4704366A (en) | 1984-06-22 | 1987-11-03 | Bio-Rad Laboratories, Inc. | Process for binding IgG to protein A |
| US4857320A (en) * | 1987-02-19 | 1989-08-15 | Monsanto Company | Method of enhancing the solubility of tissue plasminogen activator |
| JPH01238534A (ja) | 1988-03-17 | 1989-09-22 | Mitsui Toatsu Chem Inc | エンドトキシンの除去方法 |
| US5429746A (en) | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| ES2293661T3 (es) | 1996-11-27 | 2008-03-16 | Genentech, Inc. | Purificacion por afinidad de polipeptido en una matriz de proteinas a. |
| ATE370961T1 (de) | 1998-05-06 | 2007-09-15 | Genentech Inc | Reinigung von antikörpern durch ionenaustauschchromatographie |
| ES2228052T3 (es) | 1998-06-01 | 2005-04-01 | Genentech, Inc. | Separacion demonomeros de anticuerpos de sus multimeros utilizando cromatografia de intercambio de iones. |
| ES2869895T3 (es) | 2001-03-09 | 2021-10-26 | Chugai Pharmaceutical Co Ltd | Método de purificación de proteínas |
| EP1404428B1 (en) | 2001-06-05 | 2006-07-19 | Genetics Institute, LLC | Methods for purifying highly anionic proteins |
| EP1472275B1 (en) | 2002-02-05 | 2008-12-17 | Genentech, Inc. | Protein purification |
| CN100522989C (zh) | 2002-09-11 | 2009-08-05 | 中外制药株式会社 | 纯化蛋白质的方法 |
| EP1562555A2 (en) * | 2002-10-24 | 2005-08-17 | Enos Pharmaceuticals, Inc. | Sustained release l-arginine formulations and methods of manufacture and use |
| MXPA05005558A (es) * | 2002-11-26 | 2005-07-26 | Protein Design Labs Inc | Anticuerpos quimericos y humanizados para integrina ( 5 ( 1 que modulan la angiogenesis. |
| ES2321297T3 (es) * | 2003-01-08 | 2009-06-04 | Novartis Vaccines And Diagnostics, Inc. | Composiciones acuosas estabilizadas que comprenden inhibidor de la via del factor tisular (ivft) o variante del inhibidor de la via del factor tisular. |
| GB0304576D0 (en) | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
| WO2004076485A1 (en) | 2003-02-28 | 2004-09-10 | Lonza Biologics Plc. | Antibody purification by protein a and ion exchange chromatography |
| BRPI0415887B1 (pt) | 2003-10-27 | 2021-01-26 | Wyeth | métodos para a remoção de agregados de elevado peso molecular utilizando cromatografia de hidroxiapatita |
| US8084032B2 (en) | 2004-01-21 | 2011-12-27 | Ajinomoto Co., Inc. | Purification method which prevents denaturation of an antibody |
| DE602006003108D1 (de) | 2005-03-03 | 2008-11-27 | Ajinomoto Kk | Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin |
| BRPI0608584B8 (pt) * | 2005-03-11 | 2021-05-25 | Wyeth Corp | métodos de recuperar um produto purificado |
| JP5530633B2 (ja) | 2006-01-06 | 2014-06-25 | イー・エム・デイー・ミリポア・コーポレイシヨン | アフィニティークロマトグラフィーマトリックスおよびその作成および使用法。 |
| US8263750B2 (en) | 2006-03-16 | 2012-09-11 | Amgen Inc. | Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step |
| US20100190210A1 (en) | 2006-03-20 | 2010-07-29 | Medarex, Inc. | Protein Purification |
| JP2009539767A (ja) * | 2006-06-02 | 2009-11-19 | ワイス | 精製および治療方法のためのＴＧＦ−βスーパーファミリータンパク質およびペプチドの使用 |
| US7754689B2 (en) | 2006-06-02 | 2010-07-13 | Wyeth Llc | Finger-1 peptide analogs of the TGF-β superfamily |
| JP2010501623A (ja) | 2006-08-28 | 2010-01-21 | アレス トレーディング ソシエテ アノニム | Ｆｃ含有タンパク質の精製法 |
| TW200821026A (en) | 2006-09-08 | 2008-05-16 | Wyeth Corp | Arginine wash in protein purification using affinity chromatography |
| JP2010516651A (ja) | 2007-01-17 | 2010-05-20 | メルク セローノ ソシエテ アノニム | Ｆｃ含有タンパク質の精製のための方法 |
| RU2009148891A (ru) | 2007-06-01 | 2011-07-20 | Ф.Хоффманн-Ля Рош Аг (Ch) | Очистка иммуноглобулина |
| CN103396480A (zh) | 2008-05-15 | 2013-11-20 | 诺沃—诺迪斯克有限公司 | 抗体纯化方法 |
| JP5529869B2 (ja) | 2008-08-14 | 2014-06-25 | メルク・シャープ・アンド・ドーム・コーポレーション | プロテインａアフィニティークロマトグラフィーを用いる抗体の精製方法 |
| BR112012014937B1 (pt) * | 2009-12-18 | 2021-01-19 | Novartis Ag | método de produção de um anticorpo, fragmento de anticorpo ou proteína de fusão fc purificada usando uma matriz de cromatografia de afinidade |
| KR101953405B1 (ko) | 2011-06-01 | 2019-02-28 | 노파르티스 아게 | 친화성 크로마토그래피를 위한 세척 용액 및 방법 |
-
2010
- 2010-12-17 BR BR112012014937-7A patent/BR112012014937B1/pt active IP Right Grant
- 2010-12-17 ES ES17188657T patent/ES2982297T3/es active Active
- 2010-12-17 ES ES10790452.6T patent/ES2651065T3/es active Active
- 2010-12-17 EP EP17188657.5A patent/EP3272764B1/en not_active Revoked
- 2010-12-17 JP JP2012543787A patent/JP5909450B2/ja active Active
- 2010-12-17 DK DK17188657.5T patent/DK3272764T3/da active
- 2010-12-17 LT LTEP10790452.6T patent/LT2513134T/lt unknown
- 2010-12-17 KR KR1020127015524A patent/KR101858266B1/ko active Active
- 2010-12-17 WO PCT/EP2010/070076 patent/WO2011073389A1/en not_active Ceased
- 2010-12-17 PT PT107904526T patent/PT2513134T/pt unknown
- 2010-12-17 CN CN201080057457.6A patent/CN102725304B/zh active Active
- 2010-12-17 SG SG10201408384PA patent/SG10201408384PA/en unknown
- 2010-12-17 PL PL10790452T patent/PL2513134T3/pl unknown
- 2010-12-17 RU RU2012130151/10A patent/RU2564569C2/ru active
- 2010-12-17 AU AU2010332778A patent/AU2010332778B2/en active Active
- 2010-12-17 SG SG2012044707A patent/SG181771A1/en unknown
- 2010-12-17 PT PT171886575T patent/PT3272764T/pt unknown
- 2010-12-17 LT LTEP17188657.5T patent/LT3272764T/lt unknown
- 2010-12-17 DK DK10790452.6T patent/DK2513134T3/en active
- 2010-12-17 EP EP10790452.6A patent/EP2513134B1/en not_active Revoked
- 2010-12-17 MX MX2012007103A patent/MX2012007103A/es unknown
- 2010-12-17 PL PL17188657.5T patent/PL3272764T3/pl unknown
- 2010-12-17 CA CA2784278A patent/CA2784278C/en active Active
- 2010-12-17 US US13/516,960 patent/US9505803B2/en active Active
-
2012
- 2012-06-04 IL IL220164A patent/IL220164B/en active IP Right Grant
-
2016
- 2016-02-08 JP JP2016021798A patent/JP2016128495A/ja active Pending
- 2016-10-28 US US15/336,878 patent/US20170044211A1/en not_active Abandoned
-
2019
- 2019-03-25 US US16/363,170 patent/US20190233469A1/en not_active Abandoned
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