JP2014507368A5 - - Google Patents
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- Publication number
- JP2014507368A5 JP2014507368A5 JP2013552605A JP2013552605A JP2014507368A5 JP 2014507368 A5 JP2014507368 A5 JP 2014507368A5 JP 2013552605 A JP2013552605 A JP 2013552605A JP 2013552605 A JP2013552605 A JP 2013552605A JP 2014507368 A5 JP2014507368 A5 JP 2014507368A5
- Authority
- JP
- Japan
- Prior art keywords
- apatite
- solid surface
- target molecule
- contacting
- washing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 229910052586 apatite Inorganic materials 0.000 claims description 21
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 claims description 21
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 9
- 229910001854 alkali hydroxide Inorganic materials 0.000 claims description 7
- 150000008044 alkali metal hydroxides Chemical class 0.000 claims description 7
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 claims description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 230000003472 neutralizing effect Effects 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 239000012266 salt solution Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 14
- 239000000919 ceramic Substances 0.000 claims 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- 229910052587 fluorapatite Inorganic materials 0.000 claims 2
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- -1 optionally Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical class [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 239000006167 equilibration buffer Substances 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-O oxonium Chemical compound [OH3+] XLYOFNOQVPJJNP-UHFFFAOYSA-O 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161438729P | 2011-02-02 | 2011-02-02 | |
| US61/438,729 | 2011-02-02 | ||
| PCT/US2012/023512 WO2012106449A1 (en) | 2011-02-02 | 2012-02-01 | Apatite surface neutralization with alkali solutions |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014507368A JP2014507368A (ja) | 2014-03-27 |
| JP2014507368A5 true JP2014507368A5 (enExample) | 2015-12-17 |
| JP5881744B2 JP5881744B2 (ja) | 2016-03-09 |
Family
ID=46576323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013552605A Active JP5881744B2 (ja) | 2011-02-02 | 2012-02-01 | アルカリ溶液でのアパタイトの表面中和方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US9592540B2 (enExample) |
| EP (1) | EP2670704B1 (enExample) |
| JP (1) | JP5881744B2 (enExample) |
| CA (1) | CA2825651C (enExample) |
| WO (1) | WO2012106449A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2524215B1 (en) | 2010-01-15 | 2018-12-26 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| WO2012106449A1 (en) * | 2011-02-02 | 2012-08-09 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
| WO2013122834A1 (en) | 2012-02-14 | 2013-08-22 | Bio-Rad Laboratories, Inc. | REDUCING pH EXCURSIONS IN ION EXCHANGE CHROMATOGRAPHY |
| US9815695B2 (en) | 2012-05-30 | 2017-11-14 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
| CN105392569B (zh) | 2014-06-23 | 2019-08-20 | 生物辐射实验室股份有限公司 | 磷灰石预处理 |
| EP3157551B1 (en) | 2014-06-23 | 2023-02-15 | Bio-Rad Laboratories, Inc. | Apatite in-situ restoration |
| EP3368179A4 (en) * | 2015-10-28 | 2019-06-19 | Bio-rad Laboratories, Inc. | SURFACE NEUTRALIZATION OF APATIT |
| JP2020099909A (ja) * | 2020-03-17 | 2020-07-02 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 処理方法、生産方法およびハイドロキシアパタイト充填剤 |
| CN113351165B (zh) * | 2021-04-29 | 2023-04-14 | 中石化石油工程技术服务有限公司 | 矿区饮用水除氟工艺 |
| CR20230586A (es) | 2021-06-04 | 2024-05-07 | Vertex Pharma | N-(hydroxyalquil (hetero)aril) tetrahidrofurano carboxamidas como moduladores de canales de sodio |
Family Cites Families (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3737516A (en) * | 1971-03-05 | 1973-06-05 | Du Pont | Calcium-deficient hydroxylapatite for use in column chromatography |
| US4053561A (en) | 1975-08-14 | 1977-10-11 | Stauffer Chemical Company | Removal of fluorine from phosphatic solutions |
| JP2507339B2 (ja) | 1986-08-11 | 1996-06-12 | 三井東圧化学株式会社 | 粗ｔＰＡの精製方法 |
| US4859342A (en) * | 1986-10-14 | 1989-08-22 | Suntory Limited | Process for industrially separating biopolymers |
| US5332503A (en) | 1993-04-16 | 1994-07-26 | Baxter International Inc. | Process for purifying collagenase |
| JP3216436B2 (ja) * | 1994-09-06 | 2001-10-09 | 三菱マテリアル株式会社 | 塩基性タンパク質吸着剤 |
| US5744587A (en) * | 1995-06-07 | 1998-04-28 | Zymogenetics, Inc. | Method for purifying thrombopoietin |
| US5783217A (en) * | 1995-11-07 | 1998-07-21 | Etex Corporation | Low temperature calcium phosphate apatite and a method of its manufacture |
| WO1999028346A1 (de) | 1997-12-03 | 1999-06-10 | Roche Diagnostics Gmbh | Erythropoietin mit hoher spezifischer aktivität |
| JP4160652B2 (ja) | 1998-05-22 | 2008-10-01 | キリンファーマ株式会社 | エンド−β−Ｎ−アセチルグルコサミニダーゼ遺伝子 |
| SK2232001A3 (en) | 1998-08-14 | 2001-08-06 | Merck & Co Inc | Process for purifying human papillomavirus virus-like particles |
| US6156178A (en) | 1999-07-13 | 2000-12-05 | Molecular Dynamics, Inc. | Increased throughput analysis of small compounds using multiple temporally spaced injections |
| CN1107116C (zh) | 2000-12-20 | 2003-04-30 | 华南理工大学 | 糖液脱色的离子交换树脂再生液及其再生方法 |
| WO2003006159A1 (fr) | 2001-07-10 | 2003-01-23 | Yoshiyuki Nagae | Matiere de revetement, peinture et procede de production de la matiere de revetement |
| CA2469815C (en) | 2001-12-21 | 2011-05-03 | Immunex Corporation | Methods for purifying protein |
| FR2834227A1 (fr) | 2001-12-27 | 2003-07-04 | Chiralsep Sarl | Materiaux supports optiquement actifs, leur procede de preparation et leurs utilisations |
| CN1311076C (zh) | 2003-04-16 | 2007-04-18 | 普罗特奥姆技术公司 | 生产重组尿激酶的方法 |
| KR101321876B1 (ko) | 2003-10-27 | 2013-10-28 | 와이어쓰 엘엘씨 | 하이드록시아파타이트 크로마토그래피를 사용한 고분자량 응집체의 제거 |
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| WO2009017491A1 (en) | 2006-06-14 | 2009-02-05 | Smithkline Beecham Corporation | Methods for purifying antibodies using ceramic hydroxyapatite |
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| JP5507242B2 (ja) | 2007-05-18 | 2014-05-28 | 公益財団法人東京都医学総合研究所 | 酵素補充療法用医薬組成物 |
| CN103157400B (zh) | 2007-06-21 | 2014-12-24 | 简·探针公司 | 用于将容器暴露于多个热区的仪器和方法 |
| EP2275431A3 (en) | 2008-01-18 | 2011-05-25 | Bio-Rad Laboratories, Inc. | Enhanced fractionation of phosphorylated and nonphosphorylated biomolecules by apatite chromotography |
| EP2265129B1 (en) * | 2008-04-08 | 2015-10-28 | Bio-Rad Laboratories, Inc. | Chromatography purification of antibodies |
| US7635791B2 (en) | 2008-04-17 | 2009-12-22 | Tpat Ip Llc | Biological buffers with wide buffering ranges |
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| EP2334699B1 (en) | 2008-09-23 | 2013-09-11 | F. Hoffmann-La Roche AG | Purification of erythropoietin |
| ES2699434T3 (es) | 2008-10-31 | 2019-02-11 | Wyeth Llc | Purificación de proteínas ácidas utilizando cromatografía de hidroxiapatita cerámica |
| WO2010126979A1 (en) * | 2009-04-29 | 2010-11-04 | Bio-Rad Laboratories, Inc. | Purification of immunoconjugates |
| HUE028341T2 (en) | 2009-06-16 | 2016-12-28 | Genzyme Corp | Improved methods for purification of recombinant aav vectors |
| EP2524215B1 (en) * | 2010-01-15 | 2018-12-26 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| US8529859B2 (en) * | 2010-03-09 | 2013-09-10 | Lawrence Livermore National Security, Llc | Method for fabricating apatite crystals and ceramics |
| US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
| WO2012106449A1 (en) * | 2011-02-02 | 2012-08-09 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
| US9815695B2 (en) * | 2012-05-30 | 2017-11-14 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
| EP3157551B1 (en) * | 2014-06-23 | 2023-02-15 | Bio-Rad Laboratories, Inc. | Apatite in-situ restoration |
| CN105392569B (zh) * | 2014-06-23 | 2019-08-20 | 生物辐射实验室股份有限公司 | 磷灰石预处理 |
| EP3368179A4 (en) * | 2015-10-28 | 2019-06-19 | Bio-rad Laboratories, Inc. | SURFACE NEUTRALIZATION OF APATIT |
-
2012
- 2012-02-01 WO PCT/US2012/023512 patent/WO2012106449A1/en not_active Ceased
- 2012-02-01 US US13/363,670 patent/US9592540B2/en active Active
- 2012-02-01 CA CA2825651A patent/CA2825651C/en active Active
- 2012-02-01 JP JP2013552605A patent/JP5881744B2/ja active Active
- 2012-02-01 EP EP12742721.9A patent/EP2670704B1/en active Active
-
2017
- 2017-02-14 US US15/432,755 patent/US9737829B2/en active Active
- 2017-07-19 US US15/654,275 patent/US9950279B2/en active Active
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