JP2013517284A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2013517284A5 JP2013517284A5 JP2012549083A JP2012549083A JP2013517284A5 JP 2013517284 A5 JP2013517284 A5 JP 2013517284A5 JP 2012549083 A JP2012549083 A JP 2012549083A JP 2012549083 A JP2012549083 A JP 2012549083A JP 2013517284 A5 JP2013517284 A5 JP 2013517284A5
- Authority
- JP
- Japan
- Prior art keywords
- target molecule
- solution
- apatite
- solid surface
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 45
- 239000007787 solid Substances 0.000 claims description 22
- -1 amino compound Chemical class 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 18
- 229910052586 apatite Inorganic materials 0.000 claims description 15
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 claims description 15
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 239000000919 ceramic Substances 0.000 claims description 9
- 229910001413 alkali metal ion Inorganic materials 0.000 claims description 8
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims description 6
- 229910052587 fluorapatite Inorganic materials 0.000 claims description 5
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 5
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 5
- 150000002632 lipids Chemical class 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 239000004475 Arginine Substances 0.000 claims description 3
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 3
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 3
- 239000004472 Lysine Substances 0.000 claims description 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 claims description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 claims description 2
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 claims description 2
- 239000007991 ACES buffer Substances 0.000 claims description 2
- 239000007995 HEPES buffer Substances 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 2
- 239000007987 MES buffer Substances 0.000 claims description 2
- 239000007993 MOPS buffer Substances 0.000 claims description 2
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical compound NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 claims description 2
- 239000007990 PIPES buffer Substances 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical group [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- 238000010828 elution Methods 0.000 claims description 2
- 239000002158 endotoxin Substances 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 229910052744 lithium Inorganic materials 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 230000000717 retained effect Effects 0.000 claims description 2
- 150000003384 small molecules Chemical class 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 239000007983 Tris buffer Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29549910P | 2010-01-15 | 2010-01-15 | |
| US61/295,499 | 2010-01-15 | ||
| PCT/US2011/021158 WO2011088225A1 (en) | 2010-01-15 | 2011-01-13 | Surface neutralization of apatite |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013517284A JP2013517284A (ja) | 2013-05-16 |
| JP2013517284A5 true JP2013517284A5 (enExample) | 2014-01-30 |
| JP5773454B2 JP5773454B2 (ja) | 2015-09-02 |
Family
ID=44278020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012549083A Active JP5773454B2 (ja) | 2010-01-15 | 2011-01-13 | アパタイトの表面中和方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (4) | US8951807B2 (enExample) |
| EP (1) | EP2524215B1 (enExample) |
| JP (1) | JP5773454B2 (enExample) |
| CA (1) | CA2787009C (enExample) |
| WO (1) | WO2011088225A1 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2524215B1 (en) | 2010-01-15 | 2018-12-26 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
| WO2012106449A1 (en) | 2011-02-02 | 2012-08-09 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
| WO2013043608A1 (en) * | 2011-09-20 | 2013-03-28 | Bio-Rad Laboratories, Inc. | Removal of virucidal agents from biomolecule preparations |
| WO2013122834A1 (en) | 2012-02-14 | 2013-08-22 | Bio-Rad Laboratories, Inc. | REDUCING pH EXCURSIONS IN ION EXCHANGE CHROMATOGRAPHY |
| EP2855501B1 (en) | 2012-05-30 | 2018-04-25 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
| JP6325446B2 (ja) * | 2012-09-18 | 2018-05-16 | 株式会社小糸製作所 | 吸着方法およびドラッグデリバリー用担持体 |
| AU2013334644B2 (en) * | 2012-10-24 | 2018-07-12 | Genzyme Corporation | Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers |
| EP3157689B1 (en) * | 2014-06-23 | 2021-04-28 | Bio-Rad Laboratories, Inc. | Apatite pretreatment |
| CN105407917B (zh) | 2014-06-23 | 2021-07-06 | 生物辐射实验室股份有限公司 | 磷灰石原位恢复 |
| WO2017074767A1 (en) * | 2015-10-28 | 2017-05-04 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| JP7137816B2 (ja) * | 2017-04-27 | 2022-09-15 | 国立研究開発法人産業技術総合研究所 | 塩基性の部位を備える物質を選択的に吸着する吸着材 |
| JP6951051B2 (ja) * | 2018-05-24 | 2021-10-20 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 吸着剤の生産方法 |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3737516A (en) * | 1971-03-05 | 1973-06-05 | Du Pont | Calcium-deficient hydroxylapatite for use in column chromatography |
| US4053561A (en) * | 1975-08-14 | 1977-10-11 | Stauffer Chemical Company | Removal of fluorine from phosphatic solutions |
| JP2507339B2 (ja) | 1986-08-11 | 1996-06-12 | 三井東圧化学株式会社 | 粗ｔＰＡの精製方法 |
| US4859342A (en) * | 1986-10-14 | 1989-08-22 | Suntory Limited | Process for industrially separating biopolymers |
| US5332503A (en) * | 1993-04-16 | 1994-07-26 | Baxter International Inc. | Process for purifying collagenase |
| US5744587A (en) * | 1995-06-07 | 1998-04-28 | Zymogenetics, Inc. | Method for purifying thrombopoietin |
| US5783217A (en) * | 1995-11-07 | 1998-07-21 | Etex Corporation | Low temperature calcium phosphate apatite and a method of its manufacture |
| KR100390325B1 (ko) | 1997-12-03 | 2003-07-07 | 로셰 디아그노스틱스 게엠베하 | 높은 특이활성을 가진 에리트로포이에틴 |
| JP4160652B2 (ja) | 1998-05-22 | 2008-10-01 | キリンファーマ株式会社 | エンド−β−Ｎ−アセチルグルコサミニダーゼ遺伝子 |
| PL347472A1 (en) * | 1998-08-14 | 2002-04-08 | Merck & Co Inc | Process for purifying human papillomavirus virus-like particles |
| US6156178A (en) | 1999-07-13 | 2000-12-05 | Molecular Dynamics, Inc. | Increased throughput analysis of small compounds using multiple temporally spaced injections |
| CN1547507A (zh) | 2001-07-10 | 2004-11-17 | 涂布剂、涂料和涂布剂的制造方法 | |
| WO2003059935A2 (en) | 2001-12-21 | 2003-07-24 | Immunex Corporation | Methods for purifying protein |
| FR2834227A1 (fr) | 2001-12-27 | 2003-07-04 | Chiralsep Sarl | Materiaux supports optiquement actifs, leur procede de preparation et leurs utilisations |
| CN1311076C (zh) * | 2003-04-16 | 2007-04-18 | 普罗特奥姆技术公司 | 生产重组尿激酶的方法 |
| AU2004286938B2 (en) * | 2003-10-27 | 2011-06-30 | Wyeth | Removal of high molecular weight aggregates using hydroxyapatite chromatography |
| DK1841863T3 (da) * | 2005-01-14 | 2010-11-29 | Bayer Healthcare Llc | Fremgangsmåde til rensning af faktor VII |
| KR101482791B1 (ko) | 2005-03-11 | 2015-01-21 | 와이어쓰 엘엘씨 | 약한 분배성 크로마토그래피법 |
| US20060246544A1 (en) * | 2005-03-30 | 2006-11-02 | Neose Technologies,Inc. | Manufacturing process for the production of peptides grown in insect cell lines |
| US20100234577A1 (en) | 2006-06-14 | 2010-09-16 | Smithkline Beecham Corporation | Methods for purifying antibodies using ceramic hydroxyapatite |
| ES2755386T5 (es) | 2006-08-28 | 2023-04-05 | Ares Trading Sa | Proceso para la purificación de proteínas que contienen fragmentos Fc |
| KR20170001720A (ko) | 2007-03-14 | 2017-01-04 | 다케다 백신즈 인코포레이티드 | 바이러스 유사 입자 정제 |
| TWI461434B (zh) | 2007-03-23 | 2014-11-21 | Asahi Kasei Pharma Corp | Preparation of high purity soluble thrombin modulators |
| JP5507242B2 (ja) | 2007-05-18 | 2014-05-28 | 公益財団法人東京都医学総合研究所 | 酵素補充療法用医薬組成物 |
| EP2175999B1 (en) | 2007-06-21 | 2017-01-04 | Gen-Probe Incorporated | Receptacles for use in performing processes |
| EP2287174B8 (en) * | 2008-01-18 | 2016-12-07 | Bio-Rad Laboratories, Inc. | Enhanced purification of antibody fragments by apatite chromatography |
| CA2712606A1 (en) | 2008-02-08 | 2009-08-13 | Ambrx, Inc. | Modified leptin polypeptides and their uses |
| US7635791B2 (en) * | 2008-04-17 | 2009-12-22 | Tpat Ip Llc | Biological buffers with wide buffering ranges |
| US20090318674A1 (en) * | 2008-06-03 | 2009-12-24 | Gagnon Peter S | Process for purification of antibodies |
| US20120172299A1 (en) | 2008-09-23 | 2012-07-05 | F. Hoffmann-La Roche Ag | Purification of erythropoietin |
| EP2350130B1 (en) * | 2008-10-31 | 2018-10-03 | Wyeth LLC | Purification of acidic proteins using ceramic hydroxyapatite chromatography |
| CN102803478B (zh) | 2009-06-16 | 2016-04-27 | 建新公司 | 用于纯化重组aav载体的改进方法 |
| EP2524215B1 (en) * | 2010-01-15 | 2018-12-26 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
| WO2012106449A1 (en) | 2011-02-02 | 2012-08-09 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
| EP2855501B1 (en) | 2012-05-30 | 2018-04-25 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
-
2011
- 2011-01-13 EP EP11733384.9A patent/EP2524215B1/en active Active
- 2011-01-13 US US13/006,022 patent/US8951807B2/en active Active
- 2011-01-13 JP JP2012549083A patent/JP5773454B2/ja active Active
- 2011-01-13 WO PCT/US2011/021158 patent/WO2011088225A1/en not_active Ceased
- 2011-01-13 CA CA2787009A patent/CA2787009C/en active Active
-
2015
- 2015-01-16 US US14/598,719 patent/US9212203B2/en active Active
- 2015-11-04 US US14/932,080 patent/US9914749B2/en active Active
-
2018
- 2018-01-24 US US15/879,239 patent/US10676502B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2013517284A5 (enExample) | ||
| JP5773454B2 (ja) | アパタイトの表面中和方法 | |
| JP5624476B2 (ja) | アパタイトクロマトグラフィーによるリン酸化生体分子および非リン酸化生体分子の向上した精製法 | |
| JP2011510303A5 (enExample) | ||
| JP2007532477A5 (enExample) | ||
| JP2014507368A5 (enExample) | ||
| JP2013500711A5 (enExample) | ||
| JP6410734B2 (ja) | タンパク質調製物の凝集体含有量を低減する方法 | |
| JP2012018171A5 (enExample) | ||
| JP5881744B2 (ja) | アルカリ溶液でのアパタイトの表面中和方法 | |
| CN105392569B (zh) | 磷灰石预处理 | |
| US20120202975A1 (en) | Elution of proteins from hydroxyapatite resins without resin deterioration | |
| JP5903101B2 (ja) | クロマトグラフィーにおける生成物と複合体形成した夾雑物の分離方法 | |
| EP3157551A1 (en) | Apatite in-situ restoration | |
| He et al. | Preparation of a novel Zr4+-immobilized metal affinity membrane for selective adsorption of phosphoprotein | |
| IL297108A (en) | A method for purifying single-stranded RNA | |
| US20170121365A1 (en) | Surface neutralization of apatite | |
| JP2020506933A5 (enExample) | ||
| Gagnon et al. | The practical task of monoclonal IgG purification with CHT™ ceramic hydroxyapatite | |
| Allmaier et al. | Competitive Peptide Adsorption to Nitrocellulose Covered Backing Material in Cf-252-Plasma Desorption |