JP2020506933A5 - - Google Patents

Info

Publication number

JP2020506933A5

JP2020506933A5 JP2019542078A JP2019542078A JP2020506933A5 JP 2020506933 A5 JP2020506933 A5 JP 2020506933A5 JP 2019542078 A JP2019542078 A JP 2019542078A JP 2019542078 A JP2019542078 A JP 2019542078A JP 2020506933 A5 JP2020506933 A5 JP 2020506933A5

Authority

JP

Japan

Prior art keywords

solid phase

buffer

concentration

protein

target

Prior art date

2018-02-06

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 238000000034 method Methods 0.000 claims 41
• 108090000623 proteins and genes Proteins 0.000 claims 12
• 102000004169 proteins and genes Human genes 0.000 claims 12
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 11
• 239000007790 solid phase Substances 0.000 claims 11
• 239000000872 buffer Substances 0.000 claims 9
• 235000002639 sodium chloride Nutrition 0.000 claims 9
• NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 8
• 239000007983 Tris buffer Substances 0.000 claims 6
• 238000004587 chromatography analysis Methods 0.000 claims 6
• 239000011780 sodium chloride Substances 0.000 claims 6
• 238000005349 anion exchange Methods 0.000 claims 5
• 229910052588 hydroxylapatite Inorganic materials 0.000 claims 5
• 239000000203 mixture Substances 0.000 claims 5
• XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 5
• 102100034925 P-selectin glycoprotein ligand 1 Human genes 0.000 claims 4
• 229910000147 aluminium phosphate Inorganic materials 0.000 claims 4
• 238000010828 elution Methods 0.000 claims 4
• 230000002209 hydrophobic effect Effects 0.000 claims 4
• 101710137390 P-selectin glycoprotein ligand 1 Proteins 0.000 claims 3
• 230000003993 interaction Effects 0.000 claims 3
• 150000003839 salts Chemical class 0.000 claims 3
• 102100026398 Cyclic AMP-responsive element-binding protein 3 Human genes 0.000 claims 2
• 101000855520 Homo sapiens Cyclic AMP-responsive element-binding protein 3 Proteins 0.000 claims 2
• SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 claims 2
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 2
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 2
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims 1
• DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 claims 1
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
• 239000007995 HEPES buffer Substances 0.000 claims 1
• 239000007993 MOPS buffer Substances 0.000 claims 1
• JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 claims 1
• 108010054395 P-selectin ligand protein Proteins 0.000 claims 1
• 229910019142 PO4 Inorganic materials 0.000 claims 1
• UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 claims 1
• 239000007994 TES buffer Substances 0.000 claims 1
• 239000007997 Tricine buffer Substances 0.000 claims 1
• GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims 1
• 150000001413 amino acids Chemical group 0.000 claims 1
• BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 1
• 229910052921 ammonium sulfate Inorganic materials 0.000 claims 1
• 239000001166 ammonium sulphate Substances 0.000 claims 1
• 235000011130 ammonium sulphate Nutrition 0.000 claims 1
• 238000000502 dialysis Methods 0.000 claims 1
• KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 claims 1
• 238000010790 dilution Methods 0.000 claims 1
• 239000012895 dilution Substances 0.000 claims 1
• 238000006471 dimerization reaction Methods 0.000 claims 1
• 238000001914 filtration Methods 0.000 claims 1
• 239000012160 loading buffer Substances 0.000 claims 1
• 239000010452 phosphate Substances 0.000 claims 1
• 229940085991 phosphate ion Drugs 0.000 claims 1
• OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims 1
• 229910052939 potassium sulfate Inorganic materials 0.000 claims 1
• 239000001120 potassium sulphate Substances 0.000 claims 1
• 235000011151 potassium sulphates Nutrition 0.000 claims 1
• 238000002305 strong-anion-exchange chromatography Methods 0.000 claims 1