CA2787009C - Surface neutralization of apatite - Google Patents

Surface neutralization of apatite Download PDF

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Publication number
CA2787009C
CA2787009C CA2787009A CA2787009A CA2787009C CA 2787009 C CA2787009 C CA 2787009C CA 2787009 A CA2787009 A CA 2787009A CA 2787009 A CA2787009 A CA 2787009A CA 2787009 C CA2787009 C CA 2787009C
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CA
Canada
Prior art keywords
apatite
target molecule
solution
column
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2787009A
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English (en)
French (fr)
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CA2787009A1 (en
Inventor
Larry J. Cummings
Mark A. Snyder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CA2787009A1 publication Critical patent/CA2787009A1/en
Application granted granted Critical
Publication of CA2787009C publication Critical patent/CA2787009C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
    • B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Inorganic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CA2787009A 2010-01-15 2011-01-13 Surface neutralization of apatite Active CA2787009C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29549910P 2010-01-15 2010-01-15
US61/295,499 2010-01-15
PCT/US2011/021158 WO2011088225A1 (en) 2010-01-15 2011-01-13 Surface neutralization of apatite

Publications (2)

Publication Number Publication Date
CA2787009A1 CA2787009A1 (en) 2011-07-21
CA2787009C true CA2787009C (en) 2018-04-03

Family

ID=44278020

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2787009A Active CA2787009C (en) 2010-01-15 2011-01-13 Surface neutralization of apatite

Country Status (5)

Country Link
US (4) US8951807B2 (enExample)
EP (1) EP2524215B1 (enExample)
JP (1) JP5773454B2 (enExample)
CA (1) CA2787009C (enExample)
WO (1) WO2011088225A1 (enExample)

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JP5773454B2 (ja) 2010-01-15 2015-09-02 バイオ−ラッド ラボラトリーズ インコーポレーティッド アパタイトの表面中和方法
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
WO2012106449A1 (en) * 2011-02-02 2012-08-09 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
WO2013043608A1 (en) * 2011-09-20 2013-03-28 Bio-Rad Laboratories, Inc. Removal of virucidal agents from biomolecule preparations
CN104136121B (zh) * 2012-02-14 2017-04-19 生物辐射实验室股份有限公司 减少离子交换色谱中的pH漂移
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
KR20150058388A (ko) * 2012-09-18 2015-05-28 가부시키가이샤 고이토 세이사꾸쇼 흡착 방법, 흡착 분리 방법 및 드러그 딜리버리용 담지체
CN104884147A (zh) 2012-10-24 2015-09-02 建新公司 使用mes和mops作为流动相改性剂从多峰树脂洗脱生物分子
US9802822B2 (en) 2014-06-23 2017-10-31 Bio-Rad Laboratories, Inc. Apatite pretreatment
US10099157B2 (en) 2014-06-23 2018-10-16 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
CN108348820A (zh) * 2015-10-28 2018-07-31 生物辐射实验室股份有限公司 磷灰石的表面中和
JP7137816B2 (ja) * 2017-04-27 2022-09-15 国立研究開発法人産業技術総合研究所 塩基性の部位を備える物質を選択的に吸着する吸着材
WO2019224660A1 (ja) * 2018-05-24 2019-11-28 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 吸着剤の生産方法

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US4053561A (en) 1975-08-14 1977-10-11 Stauffer Chemical Company Removal of fluorine from phosphatic solutions
JP2507339B2 (ja) 1986-08-11 1996-06-12 三井東圧化学株式会社 粗ｔＰＡの精製方法
US4859342A (en) 1986-10-14 1989-08-22 Suntory Limited Process for industrially separating biopolymers
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JP4160652B2 (ja) 1998-05-22 2008-10-01 キリンファーマ株式会社 エンド−β−Ｎ−アセチルグルコサミニダーゼ遺伝子
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FR2834227A1 (fr) 2001-12-27 2003-07-04 Chiralsep Sarl Materiaux supports optiquement actifs, leur procede de preparation et leurs utilisations
CN1311076C (zh) * 2003-04-16 2007-04-18 普罗特奥姆技术公司 生产重组尿激酶的方法
RU2409591C2 (ru) * 2003-10-27 2011-01-20 Вайет Удаление агрегатов с высокой молекулярной массой путем хроматографии на гидроксиапатитах
US20090047723A1 (en) * 2005-01-14 2009-02-19 Bayer Healthcare Llc Method for purification of factor vii
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JP5773454B2 (ja) * 2010-01-15 2015-09-02 バイオ−ラッド ラボラトリーズ インコーポレーティッド アパタイトの表面中和方法
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
WO2012106449A1 (en) 2011-02-02 2012-08-09 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins

Also Published As

Publication number Publication date
US20110178276A1 (en) 2011-07-21
EP2524215B1 (en) 2018-12-26
JP2013517284A (ja) 2013-05-16
US20160052961A1 (en) 2016-02-25
EP2524215A1 (en) 2012-11-21
US10676502B2 (en) 2020-06-09
EP2524215A4 (en) 2014-08-20
JP5773454B2 (ja) 2015-09-02
US20180155390A1 (en) 2018-06-07
CA2787009A1 (en) 2011-07-21
US8951807B2 (en) 2015-02-10
US9914749B2 (en) 2018-03-13
US9212203B2 (en) 2015-12-15
WO2011088225A1 (en) 2011-07-21
US20150191505A1 (en) 2015-07-09

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