CA2787009C - Surface neutralization of apatite - Google Patents

Surface neutralization of apatite Download PDF

Info

Publication number
CA2787009C
CA2787009C CA2787009A CA2787009A CA2787009C CA 2787009 C CA2787009 C CA 2787009C CA 2787009 A CA2787009 A CA 2787009A CA 2787009 A CA2787009 A CA 2787009A CA 2787009 C CA2787009 C CA 2787009C
Authority
CA
Canada
Prior art keywords
apatite
target molecule
solution
column
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2787009A
Other languages
English (en)
French (fr)
Other versions
CA2787009A1 (en
Inventor
Larry J. Cummings
Mark A. Snyder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CA2787009A1 publication Critical patent/CA2787009A1/en
Application granted granted Critical
Publication of CA2787009C publication Critical patent/CA2787009C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
    • B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Inorganic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CA2787009A 2010-01-15 2011-01-13 Surface neutralization of apatite Active CA2787009C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29549910P 2010-01-15 2010-01-15
US61/295,499 2010-01-15
PCT/US2011/021158 WO2011088225A1 (en) 2010-01-15 2011-01-13 Surface neutralization of apatite

Publications (2)

Publication Number Publication Date
CA2787009A1 CA2787009A1 (en) 2011-07-21
CA2787009C true CA2787009C (en) 2018-04-03

Family

ID=44278020

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2787009A Active CA2787009C (en) 2010-01-15 2011-01-13 Surface neutralization of apatite

Country Status (5)

Country Link
US (4) US8951807B2 (enExample)
EP (1) EP2524215B1 (enExample)
JP (1) JP5773454B2 (enExample)
CA (1) CA2787009C (enExample)
WO (1) WO2011088225A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2524215B1 (en) 2010-01-15 2018-12-26 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
WO2012106449A1 (en) 2011-02-02 2012-08-09 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
WO2013043608A1 (en) * 2011-09-20 2013-03-28 Bio-Rad Laboratories, Inc. Removal of virucidal agents from biomolecule preparations
WO2013122834A1 (en) 2012-02-14 2013-08-22 Bio-Rad Laboratories, Inc. REDUCING pH EXCURSIONS IN ION EXCHANGE CHROMATOGRAPHY
EP2855501B1 (en) 2012-05-30 2018-04-25 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
JP6325446B2 (ja) * 2012-09-18 2018-05-16 株式会社小糸製作所 吸着方法およびドラッグデリバリー用担持体
AU2013334644B2 (en) * 2012-10-24 2018-07-12 Genzyme Corporation Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers
EP3157689B1 (en) * 2014-06-23 2021-04-28 Bio-Rad Laboratories, Inc. Apatite pretreatment
CN105407917B (zh) 2014-06-23 2021-07-06 生物辐射实验室股份有限公司 磷灰石原位恢复
WO2017074767A1 (en) * 2015-10-28 2017-05-04 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
JP7137816B2 (ja) * 2017-04-27 2022-09-15 国立研究開発法人産業技術総合研究所 塩基性の部位を備える物質を選択的に吸着する吸着材
JP6951051B2 (ja) * 2018-05-24 2021-10-20 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 吸着剤の生産方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3737516A (en) * 1971-03-05 1973-06-05 Du Pont Calcium-deficient hydroxylapatite for use in column chromatography
US4053561A (en) * 1975-08-14 1977-10-11 Stauffer Chemical Company Removal of fluorine from phosphatic solutions
JP2507339B2 (ja) 1986-08-11 1996-06-12 三井東圧化学株式会社 粗ｔＰＡの精製方法
US4859342A (en) * 1986-10-14 1989-08-22 Suntory Limited Process for industrially separating biopolymers
US5332503A (en) * 1993-04-16 1994-07-26 Baxter International Inc. Process for purifying collagenase
US5744587A (en) * 1995-06-07 1998-04-28 Zymogenetics, Inc. Method for purifying thrombopoietin
US5783217A (en) * 1995-11-07 1998-07-21 Etex Corporation Low temperature calcium phosphate apatite and a method of its manufacture
KR100390325B1 (ko) 1997-12-03 2003-07-07 로셰 디아그노스틱스 게엠베하 높은 특이활성을 가진 에리트로포이에틴
JP4160652B2 (ja) 1998-05-22 2008-10-01 キリンファーマ株式会社 エンド−β−Ｎ−アセチルグルコサミニダーゼ遺伝子
PL347472A1 (en) * 1998-08-14 2002-04-08 Merck & Co Inc Process for purifying human papillomavirus virus-like particles
US6156178A (en) 1999-07-13 2000-12-05 Molecular Dynamics, Inc. Increased throughput analysis of small compounds using multiple temporally spaced injections
CN1547507A (zh) 2001-07-10 2004-11-17 涂布剂、涂料和涂布剂的制造方法
WO2003059935A2 (en) 2001-12-21 2003-07-24 Immunex Corporation Methods for purifying protein
FR2834227A1 (fr) 2001-12-27 2003-07-04 Chiralsep Sarl Materiaux supports optiquement actifs, leur procede de preparation et leurs utilisations
CN1311076C (zh) * 2003-04-16 2007-04-18 普罗特奥姆技术公司 生产重组尿激酶的方法
AU2004286938B2 (en) * 2003-10-27 2011-06-30 Wyeth Removal of high molecular weight aggregates using hydroxyapatite chromatography
DK1841863T3 (da) * 2005-01-14 2010-11-29 Bayer Healthcare Llc Fremgangsmåde til rensning af faktor VII
KR101482791B1 (ko) 2005-03-11 2015-01-21 와이어쓰 엘엘씨 약한 분배성 크로마토그래피법
US20060246544A1 (en) * 2005-03-30 2006-11-02 Neose Technologies,Inc. Manufacturing process for the production of peptides grown in insect cell lines
US20100234577A1 (en) 2006-06-14 2010-09-16 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
ES2755386T5 (es) 2006-08-28 2023-04-05 Ares Trading Sa Proceso para la purificación de proteínas que contienen fragmentos Fc
KR20170001720A (ko) 2007-03-14 2017-01-04 다케다 백신즈 인코포레이티드 바이러스 유사 입자 정제
TWI461434B (zh) 2007-03-23 2014-11-21 Asahi Kasei Pharma Corp Preparation of high purity soluble thrombin modulators
JP5507242B2 (ja) 2007-05-18 2014-05-28 公益財団法人東京都医学総合研究所 酵素補充療法用医薬組成物
EP2175999B1 (en) 2007-06-21 2017-01-04 Gen-Probe Incorporated Receptacles for use in performing processes
EP2287174B8 (en) * 2008-01-18 2016-12-07 Bio-Rad Laboratories, Inc. Enhanced purification of antibody fragments by apatite chromatography
CA2712606A1 (en) 2008-02-08 2009-08-13 Ambrx, Inc. Modified leptin polypeptides and their uses
US7635791B2 (en) * 2008-04-17 2009-12-22 Tpat Ip Llc Biological buffers with wide buffering ranges
US20090318674A1 (en) * 2008-06-03 2009-12-24 Gagnon Peter S Process for purification of antibodies
US20120172299A1 (en) 2008-09-23 2012-07-05 F. Hoffmann-La Roche Ag Purification of erythropoietin
EP2350130B1 (en) * 2008-10-31 2018-10-03 Wyeth LLC Purification of acidic proteins using ceramic hydroxyapatite chromatography
CN102803478B (zh) 2009-06-16 2016-04-27 建新公司 用于纯化重组aav载体的改进方法
EP2524215B1 (en) * 2010-01-15 2018-12-26 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
WO2012106449A1 (en) 2011-02-02 2012-08-09 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
EP2855501B1 (en) 2012-05-30 2018-04-25 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins

Also Published As

Publication number Publication date
US8951807B2 (en) 2015-02-10
JP5773454B2 (ja) 2015-09-02
US9212203B2 (en) 2015-12-15
EP2524215A1 (en) 2012-11-21
EP2524215A4 (en) 2014-08-20
US20160052961A1 (en) 2016-02-25
US20180155390A1 (en) 2018-06-07
US9914749B2 (en) 2018-03-13
US20110178276A1 (en) 2011-07-21
JP2013517284A (ja) 2013-05-16
CA2787009A1 (en) 2011-07-21
WO2011088225A1 (en) 2011-07-21
US10676502B2 (en) 2020-06-09
EP2524215B1 (en) 2018-12-26
US20150191505A1 (en) 2015-07-09

Similar Documents

Publication Publication Date Title
US10676502B2 (en) Surface neutralization of apatite
JP5624476B2 (ja) アパタイトクロマトグラフィーによるリン酸化生体分子および非リン酸化生体分子の向上した精製法
JP5881744B2 (ja) アルカリ溶液でのアパタイトの表面中和方法
JP5903101B2 (ja) クロマトグラフィーにおける生成物と複合体形成した夾雑物の分離方法
US10099157B2 (en) Apatite in-situ restoration
JP6892563B1 (ja) 帯電物質の精製方法
US20170121365A1 (en) Surface neutralization of apatite
CN121039144A (zh) 聚合物-羟基磷灰石复合膜

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20150819