CA2784278C - Wash solution and method for affinity chromatography - Google Patents

Wash solution and method for affinity chromatography Download PDF

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Publication number
CA2784278C
CA2784278C CA2784278A CA2784278A CA2784278C CA 2784278 C CA2784278 C CA 2784278C CA 2784278 A CA2784278 A CA 2784278A CA 2784278 A CA2784278 A CA 2784278A CA 2784278 C CA2784278 C CA 2784278C
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CA
Canada
Prior art keywords
arginine
protein
wash
wash solution
matrix
Prior art date
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Active
Application number
CA2784278A
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English (en)
French (fr)
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CA2784278A1 (en
Inventor
Achim Frauenschuh
Kurt Bill
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Novartis AG
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Novartis AG
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43598315&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2784278(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novartis AG filed Critical Novartis AG
Publication of CA2784278A1 publication Critical patent/CA2784278A1/en
Application granted granted Critical
Publication of CA2784278C publication Critical patent/CA2784278C/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2784278A 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography Active CA2784278C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28805909P 2009-12-18 2009-12-18
US61/288,059 2009-12-18
PCT/EP2010/070076 WO2011073389A1 (en) 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography

Publications (2)

Publication Number Publication Date
CA2784278A1 CA2784278A1 (en) 2011-06-23
CA2784278C true CA2784278C (en) 2019-02-26

Family

ID=43598315

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2784278A Active CA2784278C (en) 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography

Country Status (18)

Country Link
US (3) US9505803B2 (enExample)
EP (2) EP3272764B1 (enExample)
JP (2) JP5909450B2 (enExample)
KR (1) KR101858266B1 (enExample)
CN (1) CN102725304B (enExample)
AU (1) AU2010332778B2 (enExample)
BR (1) BR112012014937B1 (enExample)
CA (1) CA2784278C (enExample)
DK (2) DK3272764T3 (enExample)
ES (2) ES2982297T3 (enExample)
IL (1) IL220164B (enExample)
LT (2) LT2513134T (enExample)
MX (1) MX2012007103A (enExample)
PL (2) PL2513134T3 (enExample)
PT (2) PT2513134T (enExample)
RU (1) RU2564569C2 (enExample)
SG (2) SG10201408384PA (enExample)
WO (1) WO2011073389A1 (enExample)

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EP3455241B1 (en) 2016-05-11 2022-02-23 Cytiva BioProcess R&D AB Method of cleaning and/or sanitizing a separation matrix
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WO2017194592A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of storing a separation matrix
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CN107793469B (zh) * 2017-10-16 2021-07-30 上海药明生物技术有限公司 一种去除宿主细胞蛋白含量的亲和纯化工艺
AU2018392697B2 (en) * 2017-12-21 2025-08-14 Genzyme Corporation Methods for enhanced removal of impurities during protein a chromatography
US11155575B2 (en) 2018-03-21 2021-10-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbent, devices and methods
SG11202009629YA (en) 2018-04-02 2020-10-29 Amgen Inc Erenumab compositions and uses thereof
CN112105927B (zh) * 2018-05-08 2024-06-25 沃特世科技公司 可用于pH梯度阳离子交换色谱法的方法、组合物和试剂盒
US12103949B2 (en) * 2018-08-14 2024-10-01 Bristol-Myers Squibb Company Protein recovery
WO2021072005A1 (en) * 2019-10-08 2021-04-15 North Carolina State University Immunoglobulin purification peptides and their use
JP7817945B2 (ja) * 2020-03-27 2026-02-19 ハエマロジックス・リミテッド 組成物および方法
US20230143163A1 (en) * 2020-04-27 2023-05-11 Alexion Pharmaceuticals, Inc. Protein a chromatography purification of an antibody
CN112827217A (zh) * 2020-12-29 2021-05-25 苏州良辰生物医药科技有限公司 一种层析柱清洗液及其应用
EP4634201A1 (en) * 2022-12-12 2025-10-22 H. Lundbeck A/S Arginine wash in protein purification
WO2024233992A2 (en) * 2023-05-11 2024-11-14 Trophogen, Inc. Methods of manufacturing highly glycosylated glycoprotein hormones and their analogs
EP4729534A1 (en) 2024-10-21 2026-04-22 Technische Universität München, in Vertretung des Freistaats Bayern Polypeptides comprising protein domains linked via rigid amino acid linkers

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BR112012014937B1 (pt) * 2009-12-18 2021-01-19 Novartis Ag método de produção de um anticorpo, fragmento de anticorpo ou proteína de fusão fc purificada usando uma matriz de cromatografia de afinidade
KR101953405B1 (ko) 2011-06-01 2019-02-28 노파르티스 아게 친화성 크로마토그래피를 위한 세척 용액 및 방법

Also Published As

Publication number Publication date
BR112012014937B1 (pt) 2021-01-19
RU2564569C2 (ru) 2015-10-10
CN102725304A (zh) 2012-10-10
PL3272764T3 (pl) 2024-06-10
LT2513134T (lt) 2017-11-27
IL220164A0 (en) 2012-07-31
JP5909450B2 (ja) 2016-04-26
LT3272764T (lt) 2024-05-10
US20190233469A1 (en) 2019-08-01
PT3272764T (pt) 2024-04-24
KR20120109506A (ko) 2012-10-08
IL220164B (en) 2019-02-28
MX2012007103A (es) 2012-07-17
SG10201408384PA (en) 2015-01-29
PL2513134T3 (pl) 2018-02-28
WO2011073389A1 (en) 2011-06-23
BR112012014937A2 (pt) 2016-03-08
DK2513134T3 (en) 2017-12-18
EP3272764A1 (en) 2018-01-24
AU2010332778B2 (en) 2015-03-19
ES2651065T3 (es) 2018-01-24
KR101858266B1 (ko) 2018-05-15
US20120283416A1 (en) 2012-11-08
EP2513134A1 (en) 2012-10-24
US9505803B2 (en) 2016-11-29
EP3272764B1 (en) 2024-02-07
JP2013514073A (ja) 2013-04-25
RU2012130151A (ru) 2014-01-27
US20170044211A1 (en) 2017-02-16
EP2513134B1 (en) 2017-09-06
JP2016128495A (ja) 2016-07-14
ES2982297T3 (es) 2024-10-15
PT2513134T (pt) 2017-12-14
AU2010332778A1 (en) 2012-07-19
CA2784278A1 (en) 2011-06-23
DK3272764T3 (da) 2024-05-06
CN102725304B (zh) 2015-12-09
SG181771A1 (en) 2012-07-30

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