AU2010332778B2 - Wash solution and method for affinity chromatography - Google Patents

Wash solution and method for affinity chromatography Download PDF

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Publication number
AU2010332778B2
AU2010332778B2 AU2010332778A AU2010332778A AU2010332778B2 AU 2010332778 B2 AU2010332778 B2 AU 2010332778B2 AU 2010332778 A AU2010332778 A AU 2010332778A AU 2010332778 A AU2010332778 A AU 2010332778A AU 2010332778 B2 AU2010332778 B2 AU 2010332778B2
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Australia
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protein
arginine
wash
matrix
antibody
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AU2010332778A
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AU2010332778A1 (en
Inventor
Kurt Bill
Achim Frauenschuh
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Novartis AG
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Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2010332778A 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography Active AU2010332778B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28805909P 2009-12-18 2009-12-18
US61/288,059 2009-12-18
PCT/EP2010/070076 WO2011073389A1 (en) 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography

Publications (2)

Publication Number Publication Date
AU2010332778A1 AU2010332778A1 (en) 2012-07-19
AU2010332778B2 true AU2010332778B2 (en) 2015-03-19

Family

ID=43598315

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010332778A Active AU2010332778B2 (en) 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography

Country Status (18)

Country Link
US (3) US9505803B2 (enExample)
EP (2) EP2513134B1 (enExample)
JP (2) JP5909450B2 (enExample)
KR (1) KR101858266B1 (enExample)
CN (1) CN102725304B (enExample)
AU (1) AU2010332778B2 (enExample)
BR (1) BR112012014937B1 (enExample)
CA (1) CA2784278C (enExample)
DK (2) DK3272764T3 (enExample)
ES (2) ES2651065T3 (enExample)
IL (1) IL220164B (enExample)
LT (2) LT2513134T (enExample)
MX (1) MX2012007103A (enExample)
PL (2) PL2513134T3 (enExample)
PT (2) PT2513134T (enExample)
RU (1) RU2564569C2 (enExample)
SG (2) SG181771A1 (enExample)
WO (1) WO2011073389A1 (enExample)

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US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
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WO2017032610A1 (en) * 2015-08-21 2017-03-02 F. Hoffmann-La Roche Ag Affinity chromatography purification with low conductivity wash buffer
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US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
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US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
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JP7106187B2 (ja) 2016-05-11 2022-07-26 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを保存する方法
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US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
ES2887046T3 (es) 2017-08-17 2021-12-21 Just Evotec Biologics Inc Método de purificación de proteína glicosilada a partir de galectinas de las células hospedadoras y otros contaminantes
CN107793469B (zh) * 2017-10-16 2021-07-30 上海药明生物技术有限公司 一种去除宿主细胞蛋白含量的亲和纯化工艺
JP7786876B2 (ja) 2017-12-21 2025-12-16 ジェンザイム・コーポレーション プロテインａクロマトグラフィー中の不純物の除去を促進する方法
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
EP3774881A1 (en) 2018-04-02 2021-02-17 Amgen Inc. Erenumab compositions and uses thereof
US11612885B2 (en) * 2018-05-08 2023-03-28 Waters Technologies Corporation Methods, compositions and kits useful for pH gradient cation exchange chromatography
KR102893280B1 (ko) * 2018-08-14 2025-12-01 브리스톨-마이어스 스큅 컴퍼니 개선된 단백질 회수
US20230399358A1 (en) * 2019-10-08 2023-12-14 North Carolina State University Immunoglobulin purification peptides and their use
US20240262928A1 (en) * 2020-03-27 2024-08-08 Haemalogix Pty Ltd Composition and Method
WO2021222120A1 (en) * 2020-04-27 2021-11-04 Alexion Pharmaceuticals, Inc. Protein a chromatography purification of an antibody
CN112827217A (zh) * 2020-12-29 2021-05-25 苏州良辰生物医药科技有限公司 一种层析柱清洗液及其应用
AU2023394724A1 (en) * 2022-12-12 2025-07-31 H. Lundbeck A/S Arginine wash in protein purification
WO2024233992A2 (en) * 2023-05-11 2024-11-14 Trophogen, Inc. Methods of manufacturing highly glycosylated glycoprotein hormones and their analogs
EP4729534A1 (en) 2024-10-21 2026-04-22 Technische Universität München, in Vertretung des Freistaats Bayern Polypeptides comprising protein domains linked via rigid amino acid linkers

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WO2008031020A2 (en) * 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography

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Also Published As

Publication number Publication date
EP2513134B1 (en) 2017-09-06
EP2513134A1 (en) 2012-10-24
LT2513134T (lt) 2017-11-27
US20170044211A1 (en) 2017-02-16
WO2011073389A1 (en) 2011-06-23
SG181771A1 (en) 2012-07-30
PL3272764T3 (pl) 2024-06-10
IL220164A0 (en) 2012-07-31
AU2010332778A1 (en) 2012-07-19
BR112012014937B1 (pt) 2021-01-19
CN102725304A (zh) 2012-10-10
JP5909450B2 (ja) 2016-04-26
RU2012130151A (ru) 2014-01-27
JP2016128495A (ja) 2016-07-14
IL220164B (en) 2019-02-28
US20190233469A1 (en) 2019-08-01
EP3272764A1 (en) 2018-01-24
PT2513134T (pt) 2017-12-14
PT3272764T (pt) 2024-04-24
PL2513134T3 (pl) 2018-02-28
DK3272764T3 (da) 2024-05-06
DK2513134T3 (en) 2017-12-18
RU2564569C2 (ru) 2015-10-10
US20120283416A1 (en) 2012-11-08
US9505803B2 (en) 2016-11-29
ES2982297T3 (es) 2024-10-15
BR112012014937A2 (pt) 2016-03-08
EP3272764B1 (en) 2024-02-07
MX2012007103A (es) 2012-07-17
CA2784278A1 (en) 2011-06-23
ES2651065T3 (es) 2018-01-24
LT3272764T (lt) 2024-05-10
CN102725304B (zh) 2015-12-09
KR101858266B1 (ko) 2018-05-15
KR20120109506A (ko) 2012-10-08
JP2013514073A (ja) 2013-04-25
SG10201408384PA (en) 2015-01-29
CA2784278C (en) 2019-02-26

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