IL220164B - A method for purifying a purified antibody, antibody fragment, or fc-fused protein using an affinity chromatography matrix - Google Patents
A method for purifying a purified antibody, antibody fragment, or fc-fused protein using an affinity chromatography matrixInfo
- Publication number
- IL220164B IL220164B IL220164A IL22016412A IL220164B IL 220164 B IL220164 B IL 220164B IL 220164 A IL220164 A IL 220164A IL 22016412 A IL22016412 A IL 22016412A IL 220164 B IL220164 B IL 220164B
- Authority
- IL
- Israel
- Prior art keywords
- fusion protein
- antibody
- affinity chromatography
- chromatography matrix
- producing purified
- Prior art date
Links
- 108091006020 Fc-tagged proteins Proteins 0.000 title 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 title 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 title 1
- 238000001042 affinity chromatography Methods 0.000 title 1
- 239000012504 chromatography matrix Substances 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28805909P | 2009-12-18 | 2009-12-18 | |
| PCT/EP2010/070076 WO2011073389A1 (en) | 2009-12-18 | 2010-12-17 | Wash solution and method for affinity chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL220164A0 IL220164A0 (en) | 2012-07-31 |
| IL220164B true IL220164B (en) | 2019-02-28 |
Family
ID=43598315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL220164A IL220164B (en) | 2009-12-18 | 2012-06-04 | A method for purifying a purified antibody, antibody fragment, or fc-fused protein using an affinity chromatography matrix |
Country Status (18)
| Country | Link |
|---|---|
| US (3) | US9505803B2 (enExample) |
| EP (2) | EP2513134B1 (enExample) |
| JP (2) | JP5909450B2 (enExample) |
| KR (1) | KR101858266B1 (enExample) |
| CN (1) | CN102725304B (enExample) |
| AU (1) | AU2010332778B2 (enExample) |
| BR (1) | BR112012014937B1 (enExample) |
| CA (1) | CA2784278C (enExample) |
| DK (2) | DK3272764T3 (enExample) |
| ES (2) | ES2651065T3 (enExample) |
| IL (1) | IL220164B (enExample) |
| LT (2) | LT2513134T (enExample) |
| MX (1) | MX2012007103A (enExample) |
| PL (2) | PL3272764T3 (enExample) |
| PT (2) | PT3272764T (enExample) |
| RU (1) | RU2564569C2 (enExample) |
| SG (2) | SG181771A1 (enExample) |
| WO (1) | WO2011073389A1 (enExample) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| EP2500413A1 (en) | 2006-09-13 | 2012-09-19 | Abbott Laboratories | Cell culture improvements |
| US8895709B2 (en) | 2008-10-20 | 2014-11-25 | Abbvie Inc. | Isolation and purification of antibodies using protein A affinity chromatography |
| NZ592097A (en) | 2008-10-20 | 2013-01-25 | Abbott Lab | Viral inactivation during purification of il-12 and il-18 antibodies |
| DK3272764T3 (da) * | 2009-12-18 | 2024-05-06 | Novartis Ag | Fremgangsmåde til affinitetskromatografi |
| AU2011208659B2 (en) * | 2010-01-22 | 2015-05-28 | Boehringer Ingelheim International Gmbh | Chromatographic method for purifying Fc-containing proteins |
| PL2714714T3 (pl) * | 2011-06-01 | 2018-11-30 | Novartis Ag | Roztwór do przemywania i sposób chromatografii powinowactwa |
| MX359384B (es) | 2011-10-11 | 2018-09-25 | Genentech Inc | Conjunto mejorado de anticuerpos bisespecificos. |
| CA2910065C (en) * | 2013-05-15 | 2023-09-19 | Medimmune Limited | Purification of recombinantly produced polypeptides |
| CN105848746B (zh) * | 2013-09-05 | 2019-07-09 | 豪夫迈·罗氏有限公司 | 用于层析重复利用的方法 |
| US9546208B2 (en) | 2014-01-03 | 2017-01-17 | Bio-Rad Laboratories, Inc. | Removal of impurities from protein A eluates |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| US10695693B2 (en) | 2015-03-13 | 2020-06-30 | Bristol-Myers Squibb Company | Use of alkaline washes during chromatography to remove impurities |
| SI3337812T1 (sl) | 2015-08-21 | 2021-08-31 | F. Hoffmann-La Roche Ag | Postopek za zmanjševanje beljakovin gostiteljske celice v afinitetni kromatografiji |
| CN107849122B (zh) * | 2015-08-21 | 2022-01-25 | 豪夫迈·罗氏有限公司 | 用低电导率洗涤缓冲液进行亲和层析纯化 |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| JP6987424B2 (ja) | 2016-05-11 | 2022-01-05 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックスを洗浄および／または消毒する方法 |
| WO2017194597A1 (en) | 2016-05-11 | 2017-11-16 | Ge Healthcare Bioprocess R&D Ab | Separation matrix |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| CN109311949B (zh) | 2016-05-11 | 2022-09-16 | 思拓凡生物工艺研发有限公司 | 储存分离基质的方法 |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| KR102359192B1 (ko) * | 2016-07-25 | 2022-02-04 | 세파론, 인코포레이티드 | 친화성 크로마토그래피 세정 완충액 |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| CN111344410B (zh) | 2017-08-17 | 2023-09-15 | 济世易为生物有限公司 | 从宿主细胞半乳糖凝集素和其他污染物中纯化糖基化蛋白的方法 |
| CN107793469B (zh) * | 2017-10-16 | 2021-07-30 | 上海药明生物技术有限公司 | 一种去除宿主细胞蛋白含量的亲和纯化工艺 |
| SG11202005809TA (en) * | 2017-12-21 | 2020-07-29 | Genzyme Corp | Methods for enhanced removal of impurities during protein a chromatography |
| CN111902720B (zh) | 2018-03-21 | 2025-02-07 | 沃特世科技公司 | 基于非抗体高亲和力的样品制备、吸附剂、装置和方法 |
| MX2020010320A (es) | 2018-04-02 | 2021-01-08 | Amgen Inc | Composiciones de erenumab y usos de las mismas. |
| US11612885B2 (en) * | 2018-05-08 | 2023-03-28 | Waters Technologies Corporation | Methods, compositions and kits useful for pH gradient cation exchange chromatography |
| CN112566920A (zh) * | 2018-08-14 | 2021-03-26 | 百时美施贵宝公司 | 改善的蛋白质回收 |
| JP2022551837A (ja) * | 2019-10-08 | 2022-12-14 | ノース カロライナ ステート ユニバーシティ | 免疫グロブリン精製ペプチド、及びその使用 |
| WO2021191684A1 (en) * | 2020-03-27 | 2021-09-30 | Haemalogix Pty Ltd | Composition and method |
| US20230143163A1 (en) * | 2020-04-27 | 2023-05-11 | Alexion Pharmaceuticals, Inc. | Protein a chromatography purification of an antibody |
| CN112827217A (zh) * | 2020-12-29 | 2021-05-25 | 苏州良辰生物医药科技有限公司 | 一种层析柱清洗液及其应用 |
| KR20250121073A (ko) * | 2022-12-12 | 2025-08-11 | 하. 룬드벡 아크티에셀스카브 | 단백질 정제에서 아르기닌 세척 |
| WO2024233992A2 (en) * | 2023-05-11 | 2024-11-14 | Trophogen, Inc. | Methods of manufacturing highly glycosylated glycoprotein hormones and their analogs |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4704366A (en) | 1984-06-22 | 1987-11-03 | Bio-Rad Laboratories, Inc. | Process for binding IgG to protein A |
| US4857320A (en) * | 1987-02-19 | 1989-08-15 | Monsanto Company | Method of enhancing the solubility of tissue plasminogen activator |
| JPH01238534A (ja) * | 1988-03-17 | 1989-09-22 | Mitsui Toatsu Chem Inc | エンドトキシンの除去方法 |
| US5429746A (en) | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| ES2293661T3 (es) | 1996-11-27 | 2008-03-16 | Genentech, Inc. | Purificacion por afinidad de polipeptido en una matriz de proteinas a. |
| AU760048B2 (en) | 1998-05-06 | 2003-05-08 | Genentech Inc. | Protein purification by ion exchange chromatography |
| CA2333747C (en) | 1998-06-01 | 2008-02-19 | Genentech, Inc. | Separation of protein monomers by use of ion-exchange chromatography |
| WO2002072615A1 (en) | 2001-03-09 | 2002-09-19 | Chugai Seiyaku Kabushiki Kaisha | Method of purifying protein |
| ATE421536T1 (de) | 2001-06-05 | 2009-02-15 | Genetics Inst Llc | Verfahren zur reinigung stark anionischer proteine |
| AU2003210802B2 (en) | 2002-02-05 | 2009-09-10 | Genentech Inc. | Protein purification |
| DK1561756T3 (en) | 2002-09-11 | 2016-02-15 | Chugai Pharmaceutical Co Ltd | PROCEDURE FOR CLEANING PROTEIN. |
| CA2503284A1 (en) * | 2002-10-24 | 2004-05-06 | Enos Pharmaceuticals, Inc. | Sustained release l-arginine formulations and methods of manufacture and use |
| EP1575516B1 (en) * | 2002-11-26 | 2012-05-30 | Abbott Biotherapeutics Corp. | Chimeric and humanized antibodies to alpha5 beta1 integrin that modulate angiogenesis |
| US20040224886A1 (en) * | 2003-01-08 | 2004-11-11 | Chiron Corporation | Stabilized compositions comprising tissue factor pathway inhibitor protein or tissue factor pathway inhibitor variant proteins |
| GB0304576D0 (en) | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
| PT1601697E (pt) | 2003-02-28 | 2007-09-04 | Lonza Biologics Plc | Purificação de anticorpos através de proteína a e cromatografia de troca iónica. |
| MXPA06004472A (es) | 2003-10-27 | 2006-06-20 | Wyeth Corp | Eliminacion de agregados de atopeso molecular utilizando cromatografia de hidroxiapatita. |
| US8084032B2 (en) | 2004-01-21 | 2011-12-27 | Ajinomoto Co., Inc. | Purification method which prevents denaturation of an antibody |
| EP1698637B1 (en) | 2005-03-03 | 2008-10-15 | Ajinomoto Co., Inc. | A method for improving recovery yield in protein purification with gel filtration chromatography using arginine |
| ES2797480T3 (es) * | 2005-03-11 | 2020-12-02 | Wyeth Llc | Un procedimiento de cromatografía de reparto débil |
| JP5530633B2 (ja) | 2006-01-06 | 2014-06-25 | イー・エム・デイー・ミリポア・コーポレイシヨン | アフィニティークロマトグラフィーマトリックスおよびその作成および使用法。 |
| US8263750B2 (en) | 2006-03-16 | 2012-09-11 | Amgen Inc. | Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step |
| CA2645739A1 (en) | 2006-03-20 | 2007-09-27 | Medarex, Inc. | Protein purification |
| US7754689B2 (en) | 2006-06-02 | 2010-07-13 | Wyeth Llc | Finger-1 peptide analogs of the TGF-β superfamily |
| BRPI0712496A2 (pt) * | 2006-06-02 | 2012-09-25 | Wyeth Corp | peptìdeo isolado, composição compreendendo o referido peptìdeo, ácido nucléico isolado e métodos de purificar uma proteìna na superfamìlia do fator beta de transformação do crescimento (tgf-?) e de estabilizar uma solução contendo uma proteìna na superfamìlia tgf-b. |
| WO2008025748A1 (en) | 2006-08-28 | 2008-03-06 | Ares Trading S.A. | Process for the purification of fc-containing proteins |
| US8350013B2 (en) * | 2006-09-08 | 2013-01-08 | Wyeth Llc | Arginine wash in protein purification using affinity chromatography |
| US8168185B2 (en) | 2007-01-17 | 2012-05-01 | Merck Serono Sa | Process for the purification of anti CD-25 antibodies |
| JP5064558B2 (ja) | 2007-06-01 | 2012-10-31 | エフ．ホフマン−ラ ロシュ アーゲー | 免疫グロブリン精製 |
| CN103396480A (zh) | 2008-05-15 | 2013-11-20 | 诺沃—诺迪斯克有限公司 | 抗体纯化方法 |
| MX2011001696A (es) | 2008-08-14 | 2011-03-25 | Merck Sharp & Dohme | Metodos para purificar anticuerpos usando cromatografia de afinidad con proteina a. |
| DK3272764T3 (da) | 2009-12-18 | 2024-05-06 | Novartis Ag | Fremgangsmåde til affinitetskromatografi |
| PL2714714T3 (pl) * | 2011-06-01 | 2018-11-30 | Novartis Ag | Roztwór do przemywania i sposób chromatografii powinowactwa |
-
2010
- 2010-12-17 DK DK17188657.5T patent/DK3272764T3/da active
- 2010-12-17 PT PT171886575T patent/PT3272764T/pt unknown
- 2010-12-17 PL PL17188657.5T patent/PL3272764T3/pl unknown
- 2010-12-17 RU RU2012130151/10A patent/RU2564569C2/ru active
- 2010-12-17 CN CN201080057457.6A patent/CN102725304B/zh active Active
- 2010-12-17 SG SG2012044707A patent/SG181771A1/en unknown
- 2010-12-17 DK DK10790452.6T patent/DK2513134T3/en active
- 2010-12-17 WO PCT/EP2010/070076 patent/WO2011073389A1/en not_active Ceased
- 2010-12-17 AU AU2010332778A patent/AU2010332778B2/en active Active
- 2010-12-17 ES ES10790452.6T patent/ES2651065T3/es active Active
- 2010-12-17 CA CA2784278A patent/CA2784278C/en active Active
- 2010-12-17 EP EP10790452.6A patent/EP2513134B1/en not_active Revoked
- 2010-12-17 BR BR112012014937-7A patent/BR112012014937B1/pt active IP Right Grant
- 2010-12-17 KR KR1020127015524A patent/KR101858266B1/ko active Active
- 2010-12-17 EP EP17188657.5A patent/EP3272764B1/en active Active
- 2010-12-17 LT LTEP10790452.6T patent/LT2513134T/lt unknown
- 2010-12-17 ES ES17188657T patent/ES2982297T3/es active Active
- 2010-12-17 PT PT107904526T patent/PT2513134T/pt unknown
- 2010-12-17 LT LTEP17188657.5T patent/LT3272764T/lt unknown
- 2010-12-17 SG SG10201408384PA patent/SG10201408384PA/en unknown
- 2010-12-17 MX MX2012007103A patent/MX2012007103A/es unknown
- 2010-12-17 JP JP2012543787A patent/JP5909450B2/ja active Active
- 2010-12-17 PL PL10790452T patent/PL2513134T3/pl unknown
- 2010-12-17 US US13/516,960 patent/US9505803B2/en active Active
-
2012
- 2012-06-04 IL IL220164A patent/IL220164B/en active IP Right Grant
-
2016
- 2016-02-08 JP JP2016021798A patent/JP2016128495A/ja active Pending
- 2016-10-28 US US15/336,878 patent/US20170044211A1/en not_active Abandoned
-
2019
- 2019-03-25 US US16/363,170 patent/US20190233469A1/en not_active Abandoned
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Legal Events
| Date | Code | Title | Description |
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| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed |