JP2012503773A5 - - Google Patents

Download PDF

Info

Publication number
JP2012503773A5
JP2012503773A5 JP2011529019A JP2011529019A JP2012503773A5 JP 2012503773 A5 JP2012503773 A5 JP 2012503773A5 JP 2011529019 A JP2011529019 A JP 2011529019A JP 2011529019 A JP2011529019 A JP 2011529019A JP 2012503773 A5 JP2012503773 A5 JP 2012503773A5
Authority
JP
Japan
Prior art keywords
emulsion
cavity
droplet
sample
droplets
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2011529019A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012503773A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2009/005317 external-priority patent/WO2010036352A1/en
Publication of JP2012503773A publication Critical patent/JP2012503773A/ja
Publication of JP2012503773A5 publication Critical patent/JP2012503773A5/ja
Pending legal-status Critical Current

Links

JP2011529019A 2008-09-23 2009-09-23 液滴ベースの分析システム Pending JP2012503773A (ja)

Applications Claiming Priority (21)

Application Number Priority Date Filing Date Title
US19404308P 2008-09-23 2008-09-23
US61/194,043 2008-09-23
US20697509P 2009-02-05 2009-02-05
US61/206,975 2009-02-05
US27153809P 2009-07-21 2009-07-21
US61/271,538 2009-07-21
US27573109P 2009-09-01 2009-09-01
US61/275,731 2009-09-01
US27720009P 2009-09-21 2009-09-21
US27724909P 2009-09-21 2009-09-21
US27720309P 2009-09-21 2009-09-21
US27721609P 2009-09-21 2009-09-21
US27720409P 2009-09-21 2009-09-21
US61/277,249 2009-09-21
US61/277,203 2009-09-21
US61/277,216 2009-09-21
US61/277,200 2009-09-21
US61/277,204 2009-09-21
US27727009P 2009-09-22 2009-09-22
US61/277,270 2009-09-22
PCT/US2009/005317 WO2010036352A1 (en) 2008-09-23 2009-09-23 Droplet-based assay system

Publications (2)

Publication Number Publication Date
JP2012503773A JP2012503773A (ja) 2012-02-09
JP2012503773A5 true JP2012503773A5 (enExample) 2012-11-08

Family

ID=42060021

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011529019A Pending JP2012503773A (ja) 2008-09-23 2009-09-23 液滴ベースの分析システム

Country Status (8)

Country Link
US (2) US12168231B2 (enExample)
EP (3) EP2347245B1 (enExample)
JP (1) JP2012503773A (enExample)
CN (1) CN102405402A (enExample)
AU (1) AU2009297108B2 (enExample)
CA (4) CA2738578C (enExample)
GB (3) GB2502409B8 (enExample)
WO (1) WO2010036352A1 (enExample)

Families Citing this family (188)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3216874A1 (en) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9089844B2 (en) * 2010-11-01 2015-07-28 Bio-Rad Laboratories, Inc. System for forming emulsions
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
GB2502409B8 (en) 2008-09-23 2014-10-15 Bio Rad Laboratories Droplet-based assay system
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
WO2011120020A1 (en) * 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
EP2364368B1 (en) 2008-11-07 2014-01-15 Sequenta, Inc. Methods of monitoring conditions by sequence analysis
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
ES2568509T3 (es) 2009-01-15 2016-04-29 Adaptive Biotechnologies Corporation Perfilado de la inmunidad adaptativa y métodos para la generación de anticuerpos monoclonales
CA2765949C (en) 2009-06-25 2016-03-29 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
US9043160B1 (en) 2009-11-09 2015-05-26 Sequenta, Inc. Method of determining clonotypes and clonotype profiles
GB2485850C (en) * 2009-11-25 2019-01-23 Bio Rad Laboratories Methods and compositions for detecting copy number and chromosome aneuploidy by ligation probes and partitioning the ligated products prior to amplification
JP2013524171A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴ベースのアッセイのための液滴の発生
EP2611942A4 (en) 2010-08-31 2015-01-14 Canon Us Life Sciences Inc ANTI-TURNING AT TEMPERATURE CHANGING
AU2011305445B2 (en) 2010-09-24 2017-03-16 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
EP3483285B1 (en) 2011-02-09 2021-07-14 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
CA2830443C (en) * 2011-03-18 2021-11-16 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
WO2012139038A1 (en) * 2011-04-08 2012-10-11 Stokes Bio Limited Systems and methods for continuous flow pcr systems
JP2014512826A (ja) 2011-04-25 2014-05-29 バイオ−ラド ラボラトリーズ インコーポレイテッド 核酸分析のための方法および組成物
EP2520509B1 (en) * 2011-05-06 2017-10-04 Airbus Defence and Space GmbH Apparatus for dispensing liquid droplets into a gas flow
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
EP2760578B1 (en) 2011-09-28 2020-08-26 President and Fellows of Harvard College Systems and methods for droplet production and/or fluidic manipulation
CN103946712A (zh) * 2011-09-30 2014-07-23 不列颠哥伦比亚大学 用于流动控制润湿的方法和设备
HK1201565A1 (en) 2011-10-21 2015-09-04 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
EP3388535B1 (en) 2011-12-09 2021-03-24 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
US10077478B2 (en) 2012-03-05 2018-09-18 Adaptive Biotechnologies Corp. Determining paired immune receptor chains from frequency matched subunits
PT2831276T (pt) 2012-05-08 2016-07-26 Adaptive Biotechnologies Corp Composições e método para medição e calibração do viés de amplificação em reações pcr multiplex
US9527049B2 (en) 2012-06-20 2016-12-27 Bio-Rad Laboratories, Inc. Stabilized droplets for calibration and testing
US9422602B2 (en) 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
US9970052B2 (en) 2012-08-23 2018-05-15 Bio-Rad Laboratories, Inc. Digital assays with a generic reporter
CN102886280B (zh) * 2012-08-28 2014-06-11 博奥生物有限公司 一种微流控芯片及其应用
EP2904111B1 (en) 2012-10-01 2017-12-06 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
FR2996545B1 (fr) 2012-10-08 2016-03-25 Ecole Polytech Procede microfluidique de traitement et d'analyse d'une solution contenant un materiel biologique, et circuit microfluidique correspondant.
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US9850515B2 (en) 2013-02-08 2017-12-26 Bio-Rad Laboratories, Inc. Affinity-based partition assay for detection of target molecules
US9745571B2 (en) * 2013-03-07 2017-08-29 Bio-Rad Laboratories, Inc. Repetitive reverse transcription partition assay
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
WO2014152054A1 (en) 2013-03-15 2014-09-25 Bio-Rad Laboratories, Inc. Digital assays for mutation detection
US20160076082A1 (en) 2013-04-26 2016-03-17 Gnubio, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
US9896717B2 (en) 2013-05-09 2018-02-20 Bio-Rad Laboratories, Inc. Magnetic immuno digital PCR assay
EP3919896B1 (en) * 2013-05-23 2024-07-03 Zomedica Biotechnologies LLC Two part assembly
AU2014284148B2 (en) * 2013-06-21 2017-02-23 Bio-Rad Laboratories, Inc. Microfluidic system with fluid pickups
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
CN105555972B (zh) 2013-07-25 2020-07-31 伯乐生命医学产品有限公司 遗传测定
WO2015081102A1 (en) 2013-11-27 2015-06-04 Gnubio, Inc. Microfluidic droplet packing
EP2986761B1 (en) 2014-02-13 2018-08-15 Bio-rad Laboratories, Inc. Chromosome conformation capture in droplet partitions
CA2941612A1 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
CN113215221A (zh) 2014-04-08 2021-08-06 华盛顿大学商业中心 用于使用多分散小滴执行数字检定的方法和设备
EP3155086B1 (en) 2014-06-16 2021-10-20 Bio-Rad Laboratories, Inc. Size alternating injection into drops to facilitate sorting
US10590483B2 (en) 2014-09-15 2020-03-17 Abvitro Llc High-throughput nucleotide library sequencing
CA2966201A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
JP6393583B2 (ja) * 2014-10-30 2018-09-19 株式会社ディスコ 保護膜検出装置及び保護膜検出方法
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
EP3498866B1 (en) 2014-11-25 2026-02-25 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
RU2016151351A (ru) * 2015-01-28 2019-02-28 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Устройство для анализа, чип для анализа, набор для анализа и способ анализа с их применением
US11066698B2 (en) 2015-02-17 2021-07-20 Bio-Rad Laboratories, Inc. Small nucleic acid quantification using split cycle amplification
WO2016138122A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
EP3271849B1 (en) 2015-03-17 2021-09-08 Bio-Rad Laboratories, Inc. Detection of genome editing
FR3033715B1 (fr) * 2015-03-18 2017-04-14 R A L Diagnostics Dispositif de preparation d'une couche de cellules biologiques sur une lame et appareil de preparation automatique d'une couche de cellules utilisant ledit dispositif
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
RS60032B1 (sr) 2015-05-18 2020-04-30 Saga Diagnostics Ab Detekcija ciljne nukleinske kiseline i varijanti
CN104846100B (zh) * 2015-05-24 2017-10-10 北京工业大学 一种可用于微滴数字pcr的微滴制备方法
US10379130B2 (en) 2015-06-26 2019-08-13 Abbott Laboratories Reaction vessel exchanger device for a diagnostic analyzer
US11123740B2 (en) 2015-06-29 2021-09-21 Arizona Board Of Regents On Behalf Of Arizona State University Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis
CN107923070A (zh) 2015-08-25 2018-04-17 生物辐射实验室股份有限公司 数字式免疫测定
KR20180085717A (ko) 2015-09-24 2018-07-27 에이비비트로, 엘엘씨 친화성-올리고뉴클레오타이드 콘쥬게이트 및 그것의 사용
US20180274021A1 (en) 2015-09-24 2018-09-27 Abvitro Llc Single amplicon activated exclusion pcr
AU2016326734B2 (en) 2015-09-25 2022-07-07 Abvitro Llc High throughput process for T cell receptor target identification of natively-paired T cell receptor sequences
EP3397764A4 (en) 2015-12-30 2019-05-22 Bio-Rad Laboratories, Inc. DIGITAL PROTEIN QUANTIFICATION
EP3397765B1 (en) 2015-12-30 2023-02-01 Bio-Rad Laboratories, Inc. Method for quantitating the frequency of wild-type and mutant fragments in a nucleic acid sample
EP3397973A4 (en) * 2015-12-30 2019-06-05 Bio-Rad Laboratories, Inc. DROP ANALYSIS SYSTEM WITH AUTOMATIC CALIBRATION
CN105543073B (zh) * 2016-01-08 2019-09-20 西安交通大学 一种集成式数字核酸扩增检测系统
EP3400298B1 (en) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
JP6765826B2 (ja) * 2016-03-10 2020-10-07 シスメックス株式会社 検体処理方法、検体処理チップおよび検体処理装置
US10190961B2 (en) 2016-03-18 2019-01-29 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. Sample analyzer and sample analyzing method thereof
WO2017165620A1 (en) 2016-03-24 2017-09-28 Bio-Rad Laboratories, Inc. Use of gel beads to control droplet dispersion
WO2017183298A1 (ja) * 2016-04-20 2017-10-26 シスメックス株式会社 核酸分析装置および核酸分析方法
CN105861299B (zh) * 2016-05-05 2018-05-22 广东顺德工业设计研究院(广东顺德创新设计研究院) 微滴式数字pcr荧光检测系统和荧光检测装置
CN106018362B (zh) * 2016-05-16 2018-06-08 江苏国恒检测有限公司 一种检测苦味酸的测定器
CN105806821B (zh) * 2016-05-16 2018-06-29 青岛衡立检测有限公司 一种检测苦味酸的便携式检测器
US11946107B2 (en) 2016-06-16 2024-04-02 Bio-Rad Europe Gmbh Method of detecting Salmonella typhimurium
PT3478832T (pt) 2016-07-01 2022-05-19 Carlsberg As Método para selecionar um mutante numa população de organismos por aplicação de uma abordagem de agrupamento e divisão
EP4435116A3 (en) 2016-08-05 2024-12-18 Bio-Rad Laboratories, Inc. Second strand direct
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
AU2017332495A1 (en) 2016-09-24 2019-04-11 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
CN106501215A (zh) * 2016-10-31 2017-03-15 天津科技大学 一种微气泡体积浓度测量装置
CN110740813B (zh) 2016-11-28 2022-06-03 亚利桑那州立大学董事会 涉及连续流动液滴反应的系统和方法
US20190323095A1 (en) 2016-12-02 2019-10-24 University Of Virginia Patent Foundation Compositions and methods for absolute quantification of reverse transcriptase enzymes
CN106596489B (zh) * 2016-12-19 2019-06-28 中国科学院苏州生物医学工程技术研究所 用于荧光液滴检测中荧光强度数据的处理方法
CN118441026A (zh) 2016-12-19 2024-08-06 生物辐射实验室股份有限公司 液滴加标的相邻性保留的标签化dna
WO2018140808A1 (en) * 2017-01-26 2018-08-02 Bio-Rad Laboratories, Inc. Assay performance systems including aqueous sample stabilization
WO2018146699A1 (en) * 2017-02-07 2018-08-16 Shilps Sciences Private Limited A system for microdroplet manipulation
DE102017105194A1 (de) * 2017-03-10 2018-09-13 Little Things Factory Gmbh Fokussiereinrichtung, Tropfengenerator und Verfahren zum Erzeugen einer Vielzahl von Tröpfchen
WO2018175399A1 (en) 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers
WO2018208448A1 (en) * 2017-05-11 2018-11-15 Emd Millipore Corporation Method of maintaining narrow residence time distributions in continuous flow systems
US12049667B2 (en) 2017-05-26 2024-07-30 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
JP7010603B2 (ja) * 2017-05-31 2022-01-26 シスメックス株式会社 検体処理チップ
CN110799679A (zh) * 2017-06-20 2020-02-14 10X基因组学有限公司 用于改善液滴稳定的方法和系统
US11186862B2 (en) 2017-06-20 2021-11-30 Bio-Rad Laboratories, Inc. MDA using bead oligonucleotide
CN107312850A (zh) * 2017-07-19 2017-11-03 华东医药(杭州)基因科技有限公司 一种pcr无效扩增的检测方法
JP2019030273A (ja) * 2017-08-09 2019-02-28 キヤノン株式会社 分析システム、分析方法、プログラム、および記憶媒体
MA50079A (fr) 2017-09-07 2020-07-15 Juno Therapeutics Inc Procédés d'identification de caractéristiques cellulaires relatives à des réponses associées à une thérapie cellulaire
CN111164221B (zh) * 2017-09-27 2024-03-29 生物辐射实验室股份有限公司 数字化亲和连接试验
CN111433372B (zh) 2017-10-19 2024-01-26 生物辐射实验室股份有限公司 具有光致发光的非常规和/或反向变化的数字扩增试验
EP4180534A1 (en) 2017-11-02 2023-05-17 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
WO2019090062A1 (en) * 2017-11-03 2019-05-09 Gray Mark A Quant production and dosing
JP6897655B2 (ja) * 2017-11-13 2021-07-07 株式会社リコー デバイス及び検査方法
CN107674821A (zh) * 2017-11-16 2018-02-09 格致诊断公司 液滴温度循环反应设备
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
CN110066859A (zh) * 2018-01-24 2019-07-30 思纳福(北京)医疗科技有限公司 数字pcr检测仪
EP3739059B1 (en) * 2018-01-24 2023-12-06 Sniper (Suzhou) Life Technology Co., LTD Detection instrument for digital pcr and quantitative detection method for digital pcr
EP3746552B1 (en) 2018-01-31 2023-12-27 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
JP2019174167A (ja) * 2018-03-27 2019-10-10 株式会社エンプラス 流体取扱装置
EP3910320B1 (en) * 2018-03-29 2024-09-11 The Automation Partnership (Cambridge) Ltd. Computer-implemented method and system for spectroscopic analysis of biological material
WO2019230485A1 (ja) * 2018-05-31 2019-12-05 キヤノン株式会社 カートリッジ
JP7179494B2 (ja) * 2018-05-31 2022-11-29 キヤノン株式会社 カートリッジ
FR3082440B1 (fr) * 2018-06-14 2020-12-11 Paris Sciences Lettres Quartier Latin Methode de transfert de matiere dans un dispositif microfluidique ou millifluidique
CN112423883A (zh) * 2018-07-12 2021-02-26 卢米耐克斯公司 用于进行可变样品制备和分析过程的系统和方法
CN108709785B (zh) * 2018-08-02 2024-04-05 潍坊学院 一种用于根土复合体的新型自动土样击实仪及方法
CN113841053B (zh) * 2018-08-09 2024-05-28 港大科桥有限公司 用于将流体样本自动化处理成微流体液滴以用于体外诊断的系统
EP4249651B1 (en) 2018-08-20 2025-01-29 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
US11168373B2 (en) 2018-09-17 2021-11-09 The University Of North Carolina At Chapel Hill Method for quantifying DNA fragments in a sample by size
JP2022513059A (ja) * 2018-11-16 2022-02-07 プレシゲノム,エルエルシー 液滴生成、核酸増幅、及び検出のための一体型のマイクロ流体システム
CN111220563B (zh) * 2018-11-26 2023-02-10 吉林农业大学 一种采用红外光谱检测回收油的方法
AU2019398040A1 (en) * 2018-12-13 2021-08-05 The Royal Institution For The Advancement Of Learning/Mcgill University Ultraviolet quantitative label-free detection of dna amplification
JP2020110092A (ja) * 2019-01-13 2020-07-27 テクノグローバル株式会社 遺伝子検出システム、遺伝子検出チップ、遺伝子検出装置、遺伝子増幅方法、及び遺伝子増幅装置
US12138629B2 (en) * 2019-01-31 2024-11-12 Samplix Aps Microfluidic device and a method for provision of double emulsion droplets
DE102019108155B3 (de) 2019-03-29 2020-06-04 Leibniz-Institut Für Photonische Technologien E.V. Mikrotropfenrückhalteanordnung
EP3980536A4 (en) 2019-06-07 2023-11-15 Bio-Rad Laboratories, Inc. RESOLUTION OF MULTIPLE PARTICLES PER DROPLET
CN110308113B (zh) * 2019-07-04 2021-06-22 中南林业科技大学 一种用于近红外光谱检测的全方位翻转准球形水果装置
US12163164B2 (en) 2019-07-15 2024-12-10 Bio-Rad Laboratories, Inc. Hybrid reverse transcriptases
US20220315985A1 (en) * 2019-08-29 2022-10-06 Revosketech Inc. Emulsion composition for digital pcr and uniform partitioning method of pcr samples therefor
EP4040946A1 (en) 2019-10-10 2022-08-17 Carlsberg A/S Methods for preparing mutant plants
CN112834277B (zh) * 2019-11-22 2023-08-29 北方工业大学 一种可连续使用的流体取送样密闭接口组件
JP7417794B2 (ja) * 2019-12-10 2024-01-19 杏林製薬株式会社 核酸増幅方法、核酸増幅装置及び核酸増幅用チップ
US11060141B1 (en) 2019-12-23 2021-07-13 Stilla Technologies Multiplex drop-off digital polymerase chain reaction methods
IL296509A (en) 2020-03-16 2022-11-01 Univ North Carolina Chapel Hill Preparations and methods for selective detection of tumor-derived viral DNA
CN115666789A (zh) * 2020-03-24 2023-01-31 生物辐射实验室股份有限公司 用于热控制液滴中化学反应的方法和系统
EP4126319A1 (en) * 2020-04-01 2023-02-08 Merck Patent GmbH Emulsification device
US20230249178A1 (en) 2020-07-08 2023-08-10 Roche Sequencing Solutions, Inc. Split-pool synthesis apparatus and methods of performing split-pool synthesis
CN111986175A (zh) * 2020-08-19 2020-11-24 北京科技大学 工业喷嘴所喷液滴的粒径测量方法
JP7440905B2 (ja) * 2020-08-25 2024-02-29 株式会社オンチップ・バイオテクノロジーズ 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置
US12692558B2 (en) * 2020-08-28 2026-07-28 The Regents Of The University Of California Point of care and improved detection and quantification of biomolecules
CN112268794B (zh) * 2020-09-29 2021-08-31 中国科学院金属研究所 一种确定金属材料抗穿甲最佳微观组织状态的方法
WO2022081741A1 (en) 2020-10-15 2022-04-21 Roche Sequencing Solutions, Inc. Electrophoretic devices and methods for next-generation sequencing library preparation
WO2022087268A1 (en) 2020-10-21 2022-04-28 Bio-Rad Laboratories, Inc. Detection of pathogens in wastewater
EP4267764A1 (en) * 2020-12-22 2023-11-01 F. Hoffmann-La Roche AG Methods for performing multiplexed real-time pcr with the use of large stokes shift fluorescent dyes
US20220193674A1 (en) * 2020-12-23 2022-06-23 Imec Vzw Microfluidic Device Unit
WO2022151025A1 (zh) * 2021-01-13 2022-07-21 深圳华大智造科技股份有限公司 基于体积分布分析液滴的方法、计算机装置及存储介质
CN117098607A (zh) * 2021-02-24 2023-11-21 10X基因组学有限公司 用于浓缩乳液中的液滴的方法
WO2023059917A2 (en) 2021-10-08 2023-04-13 Bio-Rad Laboratories, Inc. B(ead-based) a(tacseq) p(rocessing)
SE2615904A1 (en) 2021-10-20 2026-07-06 Tataa Biocenter Ab Methods and compositions for detection of mutant nucleic acid sequences
US20250011849A1 (en) * 2021-10-22 2025-01-09 Hewlett-Packard Development Company, L.P. Digital droplet pcr system
KR102651120B1 (ko) * 2021-11-18 2024-03-25 한국과학기술원 표적 물질에 대한 정량 정보 제공 방법 및 이를 이용한 디바이스
KR102836699B1 (ko) * 2021-11-18 2025-07-21 한국과학기술원 표적 물질의 디지털 분석 방법 및 이를 이용한 디바이스
US11964277B2 (en) * 2022-03-10 2024-04-23 Hewlett-Packard Development Company, L.P. Metered volume microfluidic devices
AR128756A1 (es) 2022-03-11 2024-06-12 Carlsberg As Modulación de niveles de proteínas
CN114941023A (zh) * 2022-06-30 2022-08-26 杭州博日科技股份有限公司 微滴式数字pcr油相组合物、微滴式数字pcr组合物及其应用和试剂盒
CN115074236B (zh) * 2022-07-21 2022-10-25 鲲鹏基因(北京)科技有限责任公司 用于pcr仪的温控装置、扩增设备和pcr仪
CN114994139B (zh) * 2022-08-05 2022-11-08 国网天津市电力公司电力科学研究院 一种电缆缓冲层的缺陷检测方法、装置、设备及存储介质
CN116004765B (zh) * 2022-08-09 2025-11-21 合肥工业大学 一种功能性核酸探针及其制备方法与应用
TW202417109A (zh) * 2022-10-24 2024-05-01 大陸商北京劑泰醫藥科技有限公司 奈米顆粒製備
CN116223309A (zh) * 2023-02-27 2023-06-06 昆山普乐斯电子科技有限公司 一种固体材料表面滴液扩散性能的测试仪及测试方法
EP4521118A1 (en) 2023-09-06 2025-03-12 Stilla Technologies Loading unit for an analysis device
AU2024363859A1 (en) 2023-10-18 2026-04-09 Amgen Inc. Evaluation of mrna transcript levels using ddpcr for early pool and single-cell clone assessment in cell line development
EP4559568A1 (en) * 2023-11-24 2025-05-28 Sartorius BIA Separations d.o.o. Mixing unit, a mixing system comprising two or more mixing units , a method for mixing two or more fluids and a lysis system
WO2025111645A1 (en) * 2023-11-27 2025-06-05 The University Of Queensland Apparatus and associated cartridge, system and method for detecting the presence of a chemical
CN117384750B (zh) * 2023-12-06 2024-03-08 博奥生物集团有限公司 一种全集成数字化核酸分析卡盒
EP4603596A1 (en) 2024-02-16 2025-08-20 Nicolas Fernandez Chemicals, kits and methods to quantify nucleic acid
CN118980647B (zh) * 2024-08-05 2025-07-25 深圳安正检测技术有限公司 一种危险化学品液体介质在线颜色检测装置
WO2026043913A1 (en) * 2024-08-20 2026-02-26 Gen-Probe Incorporated On-board lysis, internal control, and volumetric expansion in a fluidic cartridge
CN120515297B (zh) * 2025-07-23 2025-10-17 四川大学 单分散双重乳液的微流控规模化制造方法及装备

Family Cites Families (322)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3575220A (en) 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample
BR7500831A (pt) 1974-02-11 1975-12-02 Fmc Corp Aparelho de separacao de bolhas de gas corpo de transmissao de gas para uso no aparelho processo para fabricacao do dito corpo e para difusao de bolhas finas de gas em um corpo de liquido
US4051025A (en) * 1976-09-29 1977-09-27 The United States Of America As Represented By The Department Of Health, Education And Welfare Preparative countercurrent chromatography with a slowly rotating helical tube array
US4201691A (en) 1978-01-16 1980-05-06 Exxon Research & Engineering Co. Liquid membrane generator
US4348111A (en) 1978-12-07 1982-09-07 The English Electric Company Limited Optical particle analyzers
US4283262A (en) 1980-07-01 1981-08-11 Instrumentation Laboratory Inc. Analysis system
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
US4399219A (en) 1981-01-29 1983-08-16 Massachusetts Institute Of Technology Process for isolating microbiologically active material
US4636075A (en) 1984-08-22 1987-01-13 Particle Measuring Systems, Inc. Particle measurement utilizing orthogonally polarized components of a laser beam
US4948961A (en) 1985-08-05 1990-08-14 Biotrack, Inc. Capillary flow device
US5225332A (en) 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
US5055390A (en) 1988-04-22 1991-10-08 Massachusetts Institute Of Technology Process for chemical manipulation of non-aqueous surrounded microdroplets
JPH082416B2 (ja) 1988-09-29 1996-01-17 宮崎県 エマルションの製造方法
ES2060184T3 (es) 1989-06-22 1994-11-16 Atta Moleculas anfifilicas conteniendo fluor y fosforo con propiedades tensoactivas.
GB8917963D0 (en) 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
US6176962B1 (en) 1990-02-28 2001-01-23 Aclara Biosciences, Inc. Methods for fabricating enclosed microchannel structures
GB9016163D0 (en) 1990-07-24 1990-09-05 Cemu Bioteknik Chemical method
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP0519338B1 (en) 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
US5422277A (en) 1992-03-27 1995-06-06 Ortho Diagnostic Systems Inc. Cell fixative composition and method of staining cells without destroying the cell surface
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
US5538667A (en) 1993-10-28 1996-07-23 Whitehill Oral Technologies, Inc. Ultramulsions
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5972716A (en) 1994-04-29 1999-10-26 The Perkin-Elmer Corporation Fluorescence monitoring device with textured optical tube and method for reducing background fluorescence
JP2909216B2 (ja) 1994-04-29 1999-06-23 パーキン‐エルマー コーポレイション 核酸増幅生成物のリアルタイム検出装置
DE69527585T2 (de) 1994-06-08 2003-04-03 Affymetrix, Inc. Verfahren und Vorrichtung zum Verpacken von Chips
JPH0835971A (ja) * 1994-07-25 1996-02-06 Hitachi Ltd ピペット
US5555191A (en) 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
FI100859B (sv) 1994-10-14 1998-03-13 Bjarne Holmbom Förfarande och anordning för att utföra on-line flödesextraktion av ex traherbara komponenter i vätskor
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
FR2733766B1 (fr) 1995-05-03 1997-06-06 Rhone Poulenc Rorer Sa Methode de diagnostic de la schizophrenie
US20020022261A1 (en) 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6562605B1 (en) 1995-11-13 2003-05-13 Genencor International, Inc. Extraction of water soluble biomaterials from fluids using a carbon dioxide/surfactant mixture
US5736314A (en) 1995-11-16 1998-04-07 Microfab Technologies, Inc. Inline thermo-cycler
EP0889751B1 (en) 1996-04-03 1999-09-08 The Perkin-Elmer Corporation Device and method for multiple analyte detection
JP3361530B2 (ja) 1996-06-28 2003-01-07 カリパー・テクノロジーズ・コープ. 電子ピペッタおよび電気泳動バイアスのための補償手段
CN1173776C (zh) 1996-06-28 2004-11-03 卡钳技术有限公司 在微规模流体性设备里的高通过量的筛选分析系统
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6558916B2 (en) 1996-08-02 2003-05-06 Axiom Biotechnologies, Inc. Cell flow apparatus and method for real-time measurements of patient cellular responses
GB9621357D0 (en) 1996-10-12 1996-12-04 Central Research Lab Ltd Heating apparatus
US7268179B2 (en) 1997-02-03 2007-09-11 Cytonix Corporation Hydrophobic coating compositions, articles coated with said compositions, and processes for manufacturing same
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
JP2001517948A (ja) 1997-04-01 2001-10-09 グラクソ、グループ、リミテッド 核酸配列決定法
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JP2002503336A (ja) 1997-05-16 2002-01-29 アルバータ リサーチ カウンシル 微量流通システムおよびその使用方法
US7160687B1 (en) 1997-05-29 2007-01-09 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
JP3120453B2 (ja) 1997-06-19 2000-12-25 トヨタ自動車株式会社 微小液滴の保持方法、反応方法
US5912945A (en) 1997-06-23 1999-06-15 Regents Of The University Of California X-ray compass for determining device orientation
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
US5980936A (en) 1997-08-07 1999-11-09 Alliance Pharmaceutical Corp. Multiple emulsions comprising a hydrophobic continuous phase
ES2243007T3 (es) 1997-09-16 2005-11-16 Egea Biosciences Llc Sintesis quimica completa y sintesis de genes de genomas.
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US6833242B2 (en) 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6074725A (en) 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
US7497994B2 (en) 1998-02-24 2009-03-03 Khushroo Gandhi Microfluidic devices and systems incorporating cover layers
GB9804483D0 (en) 1998-03-02 1998-04-29 Central Research Lab Ltd Apparatus for and method of controlling the rate of flow of fluid along a pathway
ES2277430T3 (es) 1998-03-04 2007-07-01 Visx Incorporated Sistema de tratamiento con laser de la presbicia.
US6175669B1 (en) 1998-03-30 2001-01-16 The Regents Of The Universtiy Of California Optical coherence domain reflectometry guidewire
JP3081880B2 (ja) 1998-03-30 2000-08-28 農林水産省食品総合研究所長 マイクロスフィアの連続製造装置
US6384915B1 (en) 1998-03-30 2002-05-07 The Regents Of The University Of California Catheter guided by optical coherence domain reflectometry
US6123798A (en) 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
CN2364458Y (zh) 1998-09-10 2000-02-16 北京万拓仪器有限公司 用于原子荧光光度计的断续流动装置
JP3012608B1 (ja) 1998-09-17 2000-02-28 農林水産省食品総合研究所長 マイクロチャネル装置及び同装置を用いたエマルションの製造方法
US6146103A (en) 1998-10-09 2000-11-14 The Regents Of The University Of California Micromachined magnetohydrodynamic actuators and sensors
US6601613B2 (en) 1998-10-13 2003-08-05 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6176609B1 (en) 1998-10-13 2001-01-23 V & P Scientific, Inc. Magnetic tumble stirring method, devices and machines for mixing in vessels
US6637463B1 (en) 1998-10-13 2003-10-28 Biomicro Systems, Inc. Multi-channel microfluidic system design with balanced fluid flow distribution
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US6261431B1 (en) 1998-12-28 2001-07-17 Affymetrix, Inc. Process for microfabrication of an integrated PCR-CE device and products produced by the same
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6522407B2 (en) 1999-01-22 2003-02-18 The Regents Of The University Of California Optical detection dental disease using polarized light
US6326083B1 (en) 1999-03-08 2001-12-04 Calipher Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6357907B1 (en) 1999-06-15 2002-03-19 V & P Scientific, Inc. Magnetic levitation stirring devices and machines for mixing in vessels
DE60038883D1 (de) 1999-06-22 2008-06-26 Tecan Trading Ag Vorrichtungen zur durchfuehrung von miniaturisierten in vitro amplifizierungsassays
WO2001007159A2 (en) 1999-07-28 2001-02-01 Genset Integration of biochemical protocols in a continuous flow microfluidic device
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
DE19964337B4 (de) 1999-10-01 2004-09-16 Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto Mikrofluidischer Mikrochip mit abbiegbarem Ansaugrohr
ATE458831T1 (de) 1999-12-07 2010-03-15 Exact Sciences Corp Verfahren zum nachweis von lungenneoplasmen in fäkalen proben
ATE340025T1 (de) 2000-01-06 2006-10-15 Caliper Life Sciences Inc Vorrichtungen und verfahren für hochdurchsatz- probenentnahme und analyse
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
JP2003522963A (ja) 2000-02-18 2003-07-29 アクララ バイオサイエンシーズ, インコーポレイテッド 複数部位反応デバイスおよび方法
MXPA02008290A (es) 2000-02-23 2004-09-10 Caliper Technologies Inc Sistema de control de presion de multi-reserva-.
JP3282619B2 (ja) 2000-03-22 2002-05-20 住友電装株式会社 ワイヤーハーネスの屈曲試験方法及び電線の屈曲試験装置
US20010048900A1 (en) 2000-05-24 2001-12-06 Bardell Ronald L. Jet vortex mixer
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6466713B2 (en) 2000-08-18 2002-10-15 The Regents Of The University Of California Optical fiber head for providing lateral viewing
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
ATE448875T1 (de) 2000-09-14 2009-12-15 Caliper Life Sciences Inc Mikrofluidische vorrichtungen und methoden um temperatur-vermittelte reaktionen durchzuführen
US7294503B2 (en) 2000-09-15 2007-11-13 California Institute Of Technology Microfabricated crossflow devices and methods
US20020142483A1 (en) 2000-10-30 2002-10-03 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
AU2002239566A1 (en) 2000-11-09 2002-05-27 Therics, Inc. Method and apparatus for obtaining information about a dispensed fluid during printing
KR100435921B1 (ko) 2000-12-29 2004-06-12 주식회사 태평양 동수역학적 이중 안정화에 의한 안정한 수-유-수 다중에멀젼 시스템 및 이의 제조방법
KR100860291B1 (ko) 2001-01-19 2008-09-25 에게아 바이오사이언시스, 인크. 표적 폴리펩티드를 암호화하는 폴리뉴클레오티드의 컴퓨터지시된 어셈블리
US7567596B2 (en) 2001-01-30 2009-07-28 Board Of Trustees Of Michigan State University Control system and apparatus for use with ultra-fast laser
AU2002243764A1 (en) 2001-01-31 2002-08-19 Sau Lan Tang Staats Microfluidic devices
EP1721658B2 (en) 2001-02-23 2020-08-05 Japan Science and Technology Agency Process and apparatus for producing microcapsules
US6949176B2 (en) * 2001-02-28 2005-09-27 Lightwave Microsystems Corporation Microfluidic control using dielectric pumping
WO2002072264A1 (en) 2001-03-09 2002-09-19 Biomicro Systems, Inc. Method and system for microfluidic interfacing to arrays
US7270786B2 (en) 2001-03-28 2007-09-18 Handylab, Inc. Methods and systems for processing microfluidic samples of particle containing fluids
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US6575188B2 (en) 2001-07-26 2003-06-10 Handylab, Inc. Methods and systems for fluid control in microfluidic devices
US7192557B2 (en) 2001-03-28 2007-03-20 Handylab, Inc. Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
AU2002307152A1 (en) 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US6793723B2 (en) 2001-05-10 2004-09-21 Pitney Bowes Inc. Homogeneous photosensitive optically variable ink compositions for ink jet printing
US20030049659A1 (en) 2001-05-29 2003-03-13 Lapidus Stanley N. Devices and methods for isolating samples into subsamples for analysis
US6905885B2 (en) 2001-06-12 2005-06-14 The Regents Of The University Of California Portable pathogen detection system
US20030013680A1 (en) 2001-06-12 2003-01-16 Keryx Methods using glycosaminoglycans for the treatment of nephropathy
US6808683B2 (en) 2001-09-25 2004-10-26 Cytonome, Inc. Droplet dispensing system
JP4537053B2 (ja) 2001-06-25 2010-09-01 ジョージア テック リサーチ コーポレーション 二重共鳴エネルギー転移核酸プローブ
US6797257B2 (en) 2001-06-26 2004-09-28 The Board Of Trustees Of The University Of Illinois Paramagnetic polymerized protein microspheres and methods of preparation thereof
US6573490B2 (en) 2001-06-28 2003-06-03 Valeo Electrical Systems, Inc. Interleaved mosaic imaging rain sensor
US20030032172A1 (en) 2001-07-06 2003-02-13 The Regents Of The University Of California Automated nucleic acid assay system
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US20030027150A1 (en) 2001-08-03 2003-02-06 Katz David A. Method of haplotyping and kit therefor
US7060227B2 (en) 2001-08-06 2006-06-13 Sau Lan Tang Staats Microfluidic devices with raised walls
AUPR707101A0 (en) 2001-08-16 2001-09-06 Corbett Research Pty Ltd Continuous flow thermal device
US7094379B2 (en) 2001-10-24 2006-08-22 Commissariat A L'energie Atomique Device for parallel and synchronous injection for sequential injection of different reagents
KR100442836B1 (ko) 2001-11-10 2004-08-02 삼성전자주식회사 생화학 유체를 온도가 다른 폐쇄된 챔버 구간을 따라 회전이동시키는 폐쇄 유체 회로 시스템
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
EP1463796B1 (en) 2001-11-30 2013-01-09 Fluidigm Corporation Microfluidic device and methods of using same
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
WO2003057010A2 (en) 2002-01-04 2003-07-17 Board Of Regents, The University Of Texas System Droplet-based microfluidic oligonucleotide synthesis engine
US20030180765A1 (en) 2002-02-01 2003-09-25 The Johns Hopkins University Digital amplification for detection of mismatch repair deficient tumor cells
US20040109793A1 (en) 2002-02-07 2004-06-10 Mcneely Michael R Three-dimensional microfluidics incorporating passive fluid control structures
US7470518B2 (en) 2002-02-12 2008-12-30 Cellectricon Ab Systems and method for rapidly changing the solution environment around sensors
DE60303504T2 (de) 2002-02-22 2006-10-12 Biodot, Inc., Irvine Verfahren und vorrichtung zur kontaktlosen tröpfchenabgabe unterhalb einer fluidischen oberfläche
CN1656234B (zh) 2002-03-20 2012-02-01 创新生物公司 包囊化核酸扩增反应混合物的微胶囊及其作为反应隔间进行平行反应的用途
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US7993266B2 (en) 2002-04-26 2011-08-09 Lawrence Livermore National Security, Llc Early detection of contagious diseases
EP1508044B1 (en) 2002-05-09 2010-09-01 The University of Chicago Device and method for pressure-driven plug transport and reaction
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
JP2005530183A (ja) 2002-06-20 2005-10-06 バイオヴェリス コーポレイション エレクトロケミルミネッセンス・フローセル及びフローセル部品
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US6794671B2 (en) 2002-07-17 2004-09-21 Particle Sizing Systems, Inc. Sensors and methods for high-sensitivity optical particle counting and sizing
US20040038385A1 (en) 2002-08-26 2004-02-26 Langlois Richard G. System for autonomous monitoring of bioagents
US7188731B2 (en) 2002-08-26 2007-03-13 The Regents Of The University Of California Variable flexure-based fluid filter
JP5695287B2 (ja) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー 微小流体の核酸解析
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US20060094108A1 (en) 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
JP4395133B2 (ja) * 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク. Dnaの単一分子増幅および検出
EP1608952B1 (en) 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
WO2004071638A2 (en) 2003-02-11 2004-08-26 Regents Of The University Of California, The Microfluidic devices and method for controlled viscous shearing and formation of amphiphilic vesicles
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
DK2308419T3 (da) 2003-03-28 2016-06-06 Inguran Llc Fremgangsmåde til bedømmelse af farvningstilstandene for dyresperm, som skal sorteres.
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
EP1615721B1 (en) * 2003-04-03 2014-06-18 Fluidigm Corporation Microfluidic devices and methods of using same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
DE10315640A1 (de) 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2004094020A2 (en) 2003-04-17 2004-11-04 Fluidigm Corporation Crystal growth devices and systems, and methods for using same
US7629123B2 (en) 2003-07-03 2009-12-08 University Of Medicine And Dentistry Of New Jersey Compositions and methods for diagnosing autism
DE602004028837D1 (de) 2003-07-03 2010-10-07 Univ New Jersey Med Gene als diagnostische werkzeuge für autismus
US8048627B2 (en) 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
AU2004262406B2 (en) * 2003-08-01 2008-07-03 Ge Healthcare Bio-Sciences Ab Optical resonance analysis unit
CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
CA2479452C (en) * 2003-08-30 2008-11-04 F.Hoffmann-La Roche Ag Method and device for determining analytes in a liquid
AU2004270220B2 (en) 2003-09-05 2009-03-05 The Chinese University Of Hong Kong Method for non-invasive prenatal diagnosis
US7417726B2 (en) 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US20050277125A1 (en) 2003-10-27 2005-12-15 Massachusetts Institute Of Technology High-density reaction chambers and methods of use
US7141537B2 (en) 2003-10-30 2006-11-28 3M Innovative Properties Company Mixture of fluorinated polyethers and use thereof as surfactant
AU2003284487A1 (en) 2003-11-28 2005-06-17 Olympus Corporation Biosubstance testing apparatus and reaction stage thereof
ES2355242T3 (es) 2003-12-05 2011-03-24 Beatrice And Samuel A. Seaver Foundation Procedimiento de evaluación del riesgo genético relativo al autismo.
WO2005073410A2 (en) 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
US8293471B2 (en) 2004-01-28 2012-10-23 Marshall University Research Corporation Apparatus and method for a continuous rapid thermal cycle system
WO2005075683A1 (en) 2004-02-03 2005-08-18 Postech Foundation High throughput device for performing continuous-flow reactions
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
CN100431679C (zh) 2004-03-23 2008-11-12 独立行政法人科学技术振兴机构 微小液滴的生成方法及装置
US7432106B2 (en) 2004-03-24 2008-10-07 Applied Biosystems Inc. Liquid processing device including gas trap, and system and method
EP2439284B1 (en) 2004-03-31 2019-05-08 The General Hospital Corporation Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments
US20050221279A1 (en) 2004-04-05 2005-10-06 The Regents Of The University Of California Method for creating chemical sensors using contact-based microdispensing technology
HU227246B1 (en) 2004-04-30 2010-12-28 Szegedi Biolog Koezpont Use of genes as molecular markers in diagnosis of schizophrenia and diagnostic kit
WO2005110395A1 (en) 2004-05-19 2005-11-24 University Of South Carolina System and device for magnetic drug targeting with magnetic drug carrier particles
WO2006085905A1 (en) * 2004-05-28 2006-08-17 Board Of Regents, The University Of Texas System Programmable fluidic processors
WO2006002167A2 (en) 2004-06-17 2006-01-05 University Of Florida Research Foudation, Inc. Multi-acceptor molecular probes and applications thereof
DE602005013153D1 (de) 2004-06-29 2009-04-23 Roche Diagnostics Gmbh Assoziation von SNPs in PPAR gamma mit Osteoporose
WO2006023719A2 (en) 2004-08-20 2006-03-02 Enh Research Institute Identification of snp’s associated with schizophrenia, schizoaffective disorder and bipolar disorder
JP5885901B2 (ja) 2004-09-09 2016-03-16 アンスティテュート キュリー マイクロチャネルまたは他のマイクロ容器中でパケットを操作するためのデバイス
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
CN101065239B (zh) * 2004-10-22 2012-02-15 住友金属矿山株式会社 阻气型透明塑料基板、其制造方法和使用其的柔性显示元件
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US8951746B2 (en) 2004-11-05 2015-02-10 Southwest Research Institute Method and devices for screening cervical cancer
US20090098044A1 (en) 2004-11-15 2009-04-16 Australian Nuclear Science And Technology Organisation Solid particles from controlled destabilisation of microemulsions
JP4601423B2 (ja) 2004-12-28 2010-12-22 独立行政法人科学技術振興機構 細胞計測および分離チップ
US20060166223A1 (en) 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
US7423751B2 (en) 2005-02-08 2008-09-09 Northrop Grumman Corporation Systems and methods for use in detecting harmful aerosol particles
WO2006086777A2 (en) 2005-02-11 2006-08-17 Memorial Sloan Kettering Cancer Center Methods and compositions for detecting a drug resistant egfr mutant
US9039273B2 (en) 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
RU2400538C2 (ru) 2005-03-05 2010-09-27 Сиджен, Инк. Олигонуклеотид с двойственной специфичностью и способы, в которых он используется
WO2006099579A2 (en) 2005-03-16 2006-09-21 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
US20070048756A1 (en) 2005-04-18 2007-03-01 Affymetrix, Inc. Methods for whole genome association studies
WO2006128098A2 (en) 2005-05-27 2006-11-30 Applera Corporation Cytometric system including nucleic acid sequence amplification, and method
DE102005037401B4 (de) * 2005-08-08 2007-09-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Bildung einer Emulsion in einem fluidischen Mikrosystem
US20070068573A1 (en) 2005-08-22 2007-03-29 Applera Corporation Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible
JP4890819B2 (ja) 2005-09-02 2012-03-07 富士通セミコンダクター株式会社 半導体装置の製造方法およびウェハ
AU2006294565A1 (en) 2005-09-30 2007-04-05 Perlegen Sciences, Inc. Methods and compositions for screening and treatment of disorders of blood glucose regulation
JP4751718B2 (ja) * 2005-12-28 2011-08-17 株式会社島津製作所 遺伝子解析装置
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US7976795B2 (en) 2006-01-19 2011-07-12 Rheonix, Inc. Microfluidic systems
WO2007089541A2 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
WO2007092473A2 (en) * 2006-02-02 2007-08-16 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
WO2007091229A1 (en) 2006-02-07 2007-08-16 Stokes Bio Limited A microfluidic droplet queuing network
US20100304446A1 (en) 2006-02-07 2010-12-02 Stokes Bio Limited Devices, systems, and methods for amplifying nucleic acids
EP2298438A1 (en) 2006-02-07 2011-03-23 Stokes Bio Limited A microfluidic droplet queuing network
EP1991357B1 (en) 2006-02-07 2016-09-14 Stokes Bio Limited A microfluidic analysis system
WO2007120745A2 (en) 2006-04-11 2007-10-25 Optiscan Biomedical Corporation Noise reduction for analyte detection system
US8492168B2 (en) 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
US7815871B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
US7816121B2 (en) * 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
AU2007237909A1 (en) * 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
US7470403B2 (en) 2006-04-26 2008-12-30 Wisconsin Alumni Research Foundation Microfluidic platform and method of generating a gradient therein
WO2007133710A2 (en) * 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
WO2007150030A2 (en) 2006-06-23 2007-12-27 Massachusetts Institute Of Technology Microfluidic synthesis of organic nanoparticles
US7608399B2 (en) 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
EP1881079A1 (en) 2006-07-20 2008-01-23 Pangaea Biotech, S.A. Method for the detection of EGFR mutations in blood samples
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
WO2008024114A1 (en) 2006-08-24 2008-02-28 Genizon Biosciences Inc. Genemap of the human genes associated with schizophrenia
WO2008030433A2 (en) 2006-09-06 2008-03-13 Canon U.S. Life Sciences, Inc. Chip and cartridge design configuration for performing micro-fluidic assays
US20080166720A1 (en) 2006-10-06 2008-07-10 The Regents Of The University Of California Method and apparatus for rapid nucleic acid analysis
EP1912067A1 (en) 2006-10-12 2008-04-16 Eppendorf Array Technologies S.A. Method for quantification of a target compound obtained from a biological sample upon chips
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
US9114398B2 (en) 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
WO2008070074A2 (en) 2006-12-04 2008-06-12 Pgxhealth Llc Genetic markers of schizophrenia
WO2008070862A2 (en) 2006-12-07 2008-06-12 Biocept, Inc. Non-invasive prenatal genetic screen
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
WO2008089244A2 (en) 2007-01-17 2008-07-24 University Of Rochester Frequency-addressable apparatus and methods for actuation of liquids
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
ES2303473B1 (es) 2007-01-26 2009-05-21 Guillermo Petri Larrea Sistema para garantizar los estiramientos de los tornillos en una union embridada y pretensada.
US20090011417A1 (en) 2007-03-07 2009-01-08 George Maltezos Testing Device
WO2008109176A2 (en) * 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2008112177A2 (en) 2007-03-08 2008-09-18 Genizon Biosciences, Inc. Genemap of the human genes associated with schizophrenia
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
JP2008245612A (ja) 2007-03-30 2008-10-16 Hitachi Ltd 試料調製法および装置
CN101663089A (zh) 2007-04-04 2010-03-03 微点生物技术有限公司 微机械加工的电润湿微流体阀
JP5666763B2 (ja) 2007-04-11 2015-02-12 味の素株式会社 油中水型乳化組成物
CA2689427C (en) 2007-06-05 2015-12-29 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
WO2009002920A1 (en) 2007-06-22 2008-12-31 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
US20090068170A1 (en) 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
CA2694820C (en) 2007-07-30 2015-06-16 F. Hoffman-La Roche Ag Methods of identification using methylation of cpg
US20090053719A1 (en) 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
ES2380844T3 (es) 2007-09-07 2012-05-18 Fluidigm Corporation Determinación de la variación en el número de copias, métodos y sistemas
EP2203567B1 (en) 2007-10-16 2011-05-11 Roche Diagnostics GmbH High resolution, high throughput hla genotyping by clonal sequencing
US7807920B2 (en) 2007-10-30 2010-10-05 Opel, Inc. Concentrated solar photovoltaic module
WO2009085246A1 (en) 2007-12-20 2009-07-09 University Of Massachusetts Cross-linked biopolymers, related compositions and methods of use
JP5224801B2 (ja) 2007-12-21 2013-07-03 キヤノン株式会社 核酸増幅装置
CA2639954C (en) 2008-02-11 2017-08-15 Aaron R. Wheeler Droplet-based cell culture and cell assays using digital microfluidics
US20090220434A1 (en) 2008-02-29 2009-09-03 Florida State University Research Foundation Nanoparticles that facilitate imaging of biological tissue and methods of forming the same
US8062903B2 (en) 2008-03-03 2011-11-22 University Of Washington Droplet compartmentalization for chemical separation and on-line sampling
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
US9011777B2 (en) 2008-03-21 2015-04-21 Lawrence Livermore National Security, Llc Monodisperse microdroplet generation and stopping without coalescence
US8961902B2 (en) 2008-04-23 2015-02-24 Bioscale, Inc. Method and apparatus for analyte processing
EP2672260A1 (en) 2008-05-13 2013-12-11 Advanced Liquid Logic, Inc. Droplet actuator devices, systems and methods
EP2352591A4 (en) 2008-06-13 2013-01-23 Emerald Biostructures Inc NANOVOLUMIC MICROKAPILLAR CRYSTALLIZATION SYSTEM
WO2010001419A2 (en) 2008-07-04 2010-01-07 Decode Genetics Ehf Copy number variations predictive of risk of schizophrenia
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US8256931B2 (en) 2008-07-24 2012-09-04 Seward George H Achromatic homogenizer and collimator for LEDs
US20100018584A1 (en) 2008-07-28 2010-01-28 Technion Research & Development Foundation Ltd. Microfluidic system and method for manufacturing the same
WO2010018465A2 (en) 2008-08-12 2010-02-18 Stokes Bio Limited Methods and devices for digital pcr
US9180453B2 (en) 2008-08-15 2015-11-10 University Of Washington Method and apparatus for the discretization and manipulation of sample volumes
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US20130084572A1 (en) 2011-09-30 2013-04-04 Quantalife, Inc. Calibrations and controls for droplet-based assays
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US20120171683A1 (en) 2010-03-02 2012-07-05 Ness Kevin D Analysis of fragmented genomic dna in droplets
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
GB2502409B8 (en) 2008-09-23 2014-10-15 Bio Rad Laboratories Droplet-based assay system
US9089844B2 (en) 2010-11-01 2015-07-28 Bio-Rad Laboratories, Inc. System for forming emulsions
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US20130059754A1 (en) 2011-09-01 2013-03-07 Bio-Rad Laboratories, Inc. Digital assays with reduced measurement uncertainty
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
EP2389455A4 (en) 2009-01-26 2012-12-05 Verinata Health Inc METHODS AND COMPOSITIONS FOR IDENTIFYING A FETAL CELL
US20100217736A1 (en) 2009-02-23 2010-08-26 Oded Sarel Decision support method and apparatus for chaotic or multi-parameter situations
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US20100261230A1 (en) 2009-04-08 2010-10-14 Applied Biosystems, Llc System comprising dual-sided thermal cycler and emulsion pcr in pouch
US8034629B2 (en) 2009-06-26 2011-10-11 Massachusetts Institute Of Technology High precision scanning of encoded hydrogel microparticles
JP5212313B2 (ja) 2009-08-24 2013-06-19 株式会社日立プラントテクノロジー 乳化装置
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
CA3041540C (en) 2009-09-21 2021-08-24 Bio-Rad Laboratories, Inc. Magnetic lysis method and device
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
EP4435111A1 (en) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP2013524171A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴ベースのアッセイのための液滴の発生
JP2013524169A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴によるアッセイ用の検出システム
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
US9266104B2 (en) 2011-02-11 2016-02-23 Raindance Technologies, Inc. Thermocycling device for nucleic acid amplification and methods of use
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
CA2830443C (en) 2011-03-18 2021-11-16 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
CN103930886A (zh) 2011-07-13 2014-07-16 伯乐生命医学产品有限公司 使用副本的meta 分析进行真实世界误差的计算
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2014039587A1 (en) 2012-09-05 2014-03-13 Bio-Rad Laboratories, Inc. Systems and methods for stabilizing droplets
SG10201710049RA (en) 2013-03-08 2018-01-30 Bio Rad Laboratories Inc Compositions, methods and systems for polymerase chain reaction assays
WO2015023677A1 (en) 2013-08-12 2015-02-19 Bio-Rad Laboratories, Inc. Amplification reporter with base-pairing oligomers
EP2883616A1 (en) 2013-12-11 2015-06-17 Alfa Laval Corporate AB Valve for draining off gas from a centrifugal separator

Similar Documents

Publication Publication Date Title
JP6669699B2 (ja) 連続的な増幅反応のための方法および装置
JP2021009159A (ja) 試料調製又は試料解析の少なくとも一方を行うため回転バルブを備えるシステム及び方法
JP6525877B2 (ja) 反応チューブ
US20220186325A1 (en) Systems for sample analysis
AU2016208331B2 (en) Plurality of reaction chambers in a test cartridge
JP2018538531A5 (enExample)
EP3954457A3 (en) Microfabricated device with micro-environment sensors for assaying coagulation in fluid samples
JP2015534810A5 (enExample)
WO2009097174A3 (en) Electrochemical biosensor
WO2012145730A3 (en) Integrated device for nucleic acid detection and identification
ITTO20120703A1 (it) Dispositivo microfluidico monouso, cartuccia includente il dispositivo microfluidico, apparecchio per eseguire una amplificazione di acido nucleico, metodo di fabbricazione del dispositivo microfluidico, e metodo di uso del dispositivo microfluidico
RU2017106623A (ru) Прибор для анализа биологических образцов и реагентов
WO2011051735A3 (en) Device and apparatus
EP2759344A3 (en) Microfluidic System for Nucleic Acid Analysis
WO2007028084A3 (en) Method and molecular diagnostic device for detection, analysis and identification of genomic dna
ATE544063T1 (de) Nanoporenvorrichtung und verfahren zur nukleinsäureanalyse
GB2477053B (en) Droplet-based assay system
EP2601286A2 (en) Pressurizable cartridge for polymerase chain reactions
JP2020534545A5 (enExample)
JP2009284769A (ja) マイクロ基板
DK2162549T3 (da) Fremgangsmåde til at detektere nukleinsyrer
NZ591711A (en) A method for predicting athletic performance potential
WO2015114316A3 (en) Fluidic device comprising immobilised reagents within a chamber
CN114196741A (zh) 一体式微液滴芯片的数字pcr方法
WO2009057829A3 (en) Nucleic acid primer set for detection of drug-resistant strain of hepatitis b virus, assay kit, and method of detecting drug-resistant strain of hepatitis b virus