IL327356A - Evaluation of mrna transcript levels using ddpcr for early pool and single-cell clone assessment in cell line development - Google Patents
Evaluation of mrna transcript levels using ddpcr for early pool and single-cell clone assessment in cell line developmentInfo
- Publication number
- IL327356A IL327356A IL327356A IL32735626A IL327356A IL 327356 A IL327356 A IL 327356A IL 327356 A IL327356 A IL 327356A IL 32735626 A IL32735626 A IL 32735626A IL 327356 A IL327356 A IL 327356A
- Authority
- IL
- Israel
- Prior art keywords
- ddpcr
- assessment
- pool
- early
- evaluation
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/101—Taqman
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202363544757P | 2023-10-18 | 2023-10-18 | |
| PCT/US2024/051847 WO2025085682A1 (en) | 2023-10-18 | 2024-10-17 | Evaluation of mrna transcript levels using ddpcr for early pool and single-cell clone assessment in cell line development |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL327356A true IL327356A (en) | 2026-05-01 |
Family
ID=93432214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL327356A IL327356A (en) | 2023-10-18 | 2024-10-17 | Evaluation of mrna transcript levels using ddpcr for early pool and single-cell clone assessment in cell line development |
Country Status (4)
| Country | Link |
|---|---|
| CN (1) | CN122055458A (en) |
| AU (1) | AU2024363859A1 (en) |
| IL (1) | IL327356A (en) |
| WO (1) | WO2025085682A1 (en) |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US4914210A (en) | 1987-10-02 | 1990-04-03 | Cetus Corporation | Oligonucleotide functionalizing reagents |
| US4965195A (en) | 1987-10-26 | 1990-10-23 | Immunex Corp. | Interleukin-7 |
| US4968607A (en) | 1987-11-25 | 1990-11-06 | Immunex Corporation | Interleukin-1 receptors |
| WO1990005183A1 (en) | 1988-10-31 | 1990-05-17 | Immunex Corporation | Interleukin-4 receptors |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| WO1991018982A1 (en) | 1990-06-05 | 1991-12-12 | Immunex Corporation | Type ii interleukin-1 receptors |
| GB9014932D0 (en) | 1990-07-05 | 1990-08-22 | Celltech Ltd | Recombinant dna product and method |
| US6319494B1 (en) | 1990-12-14 | 2001-11-20 | Cell Genesys, Inc. | Chimeric chains for receptor-associated signal transduction pathways |
| WO1994004679A1 (en) | 1991-06-14 | 1994-03-03 | Genentech, Inc. | Method for making humanized antibodies |
| JPH05244982A (en) | 1991-12-06 | 1993-09-24 | Sumitomo Chem Co Ltd | Humanized b-b10 |
| IL104570A0 (en) | 1992-03-18 | 1993-05-13 | Yeda Res & Dev | Chimeric genes and cells transformed therewith |
| US6720166B2 (en) | 1994-02-14 | 2004-04-13 | Abbott Laboratories | Non-a, non-b, non-c, non-c, non-d, non-e hepatitis reagents and methods for their use |
| JP3108105B2 (en) | 1995-08-21 | 2000-11-13 | 三光純薬株式会社 | Method for extracting coprecipitant and nucleic acid |
| JP3495375B2 (en) | 1996-01-11 | 2004-02-09 | イミュネックス・コーポレーション | Expression enhancing sequence elements (EASE) for eukaryotic expression systems |
| CA2196496A1 (en) | 1997-01-31 | 1998-07-31 | Stephen William Watson Michnick | Protein fragment complementation assay for the detection of protein-protein interactions |
| US6849431B2 (en) | 1997-07-25 | 2005-02-01 | Ryoji Tamura | Non-B, non-C, non-G hepatitis virus gene, polynucleotide, polypeptide, virus particle, method for isolating virus particle, and method for detecting virus |
| GB9722131D0 (en) | 1997-10-20 | 1997-12-17 | Medical Res Council | Method |
| GB9928787D0 (en) | 1999-12-03 | 2000-02-02 | Medical Res Council | Direct screening method |
| WO2001083823A1 (en) | 2000-04-28 | 2001-11-08 | Quantum Dot Corporation | Methods and compositions for polynucleotide analysis using generic capture sequences |
| GB0025144D0 (en) | 2000-10-13 | 2000-11-29 | Medical Res Council | Concatenated nucleic acid sequences |
| GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| US20040202995A1 (en) | 2003-04-09 | 2004-10-14 | Domantis | Nucleic acids, proteins, and screening methods |
| EP1614755A1 (en) | 2004-07-07 | 2006-01-11 | ARTEMIS Pharmaceuticals GmbH | Target transgenesis of short hairpin RNA expression cassette using recombinase mediated cassette exchange |
| EP1901068A1 (en) | 2006-09-15 | 2008-03-19 | Boehringer Ingelheim Pharma GmbH & Co. KG | Methods of selecting cell clones |
| US7935518B2 (en) | 2006-09-27 | 2011-05-03 | Alessandra Luchini | Smart hydrogel particles for biomarker harvesting |
| US8382987B2 (en) | 2006-09-27 | 2013-02-26 | Alessandra Luchini | Method for harvesting nanoparticles and sequestering biomarkers |
| US8497137B2 (en) | 2006-09-27 | 2013-07-30 | Alessandra Luchini | Smart hydrogel particles for biomarker harvesting |
| EP2129790A4 (en) * | 2007-02-01 | 2010-06-09 | Centocor Inc | Selection of high-producing cell lines |
| AU2009283194B2 (en) | 2008-08-22 | 2014-10-16 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted single-stranded cleavage and targeted integration |
| CA2738578C (en) | 2008-09-23 | 2021-05-04 | Quantalife, Inc. | Droplet-based assay system |
| CA2767056C (en) | 2009-09-02 | 2018-12-04 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
| WO2012149042A2 (en) | 2011-04-25 | 2012-11-01 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| KR20170098537A (en) * | 2016-02-22 | 2017-08-30 | 인천대학교 산학협력단 | METHOD FOR SCREENING ANTIBODY-PRODUCING CELL LINES BY LIVE CELL DETECTION OF MONOCLONAL ANTIBODY LIGHT AND HEAVY CHAIN mRNAs USING MOLECULAR BEACONS |
| US20220073945A1 (en) * | 2018-12-21 | 2022-03-10 | Merck Sharp & Dohme Corp. | Expression vectors for eukaryotic expression systems |
| US20240174752A1 (en) * | 2021-04-27 | 2024-05-30 | Amgen Inc. | Modulating product quality of asymmetric multispecific antibodies through the use of temperature |
-
2024
- 2024-10-17 IL IL327356A patent/IL327356A/en unknown
- 2024-10-17 WO PCT/US2024/051847 patent/WO2025085682A1/en active Pending
- 2024-10-17 AU AU2024363859A patent/AU2024363859A1/en active Pending
- 2024-10-17 CN CN202480066710.6A patent/CN122055458A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN122055458A (en) | 2026-05-15 |
| WO2025085682A1 (en) | 2025-04-24 |
| AU2024363859A1 (en) | 2026-04-09 |
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