JP2012501678A5 - - Google Patents

Download PDF

Info

Publication number
JP2012501678A5
JP2012501678A5 JP2011526949A JP2011526949A JP2012501678A5 JP 2012501678 A5 JP2012501678 A5 JP 2012501678A5 JP 2011526949 A JP2011526949 A JP 2011526949A JP 2011526949 A JP2011526949 A JP 2011526949A JP 2012501678 A5 JP2012501678 A5 JP 2012501678A5
Authority
JP
Japan
Prior art keywords
hydroxybutyryl
coa
microbial organism
bdo pathway
exogenous nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2011526949A
Other languages
English (en)
Other versions
JP2012501678A (ja
JP5912529B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2009/056415 external-priority patent/WO2010030711A2/en
Publication of JP2012501678A publication Critical patent/JP2012501678A/ja
Publication of JP2012501678A5 publication Critical patent/JP2012501678A5/ja
Application granted granted Critical
Publication of JP5912529B2 publication Critical patent/JP5912529B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Description

手短に述べると、アセトアセチル-CoAを3-ヒドロキシブチリル-CoAデヒドロゲナーゼ(EC 1.1.1.a)によって3-ヒドロキシブチリル-CoAに変換することができる。3-ヒドロキシブチリル-CoAを3-ヒドロキシブチリル-CoAデヒドラターゼ(EC 4.2.1.a)によってクロトノイル-CoAに変換することができる。クロトノイル-CoAをビニルアセチル-CoAΔイソメラーゼ(EC 5.3.3.3)によってビニルアセチル-CoAに変換することができる。ビニルアセチル-CoAを4-ヒドロキシブチリル-CoAデヒドラターゼ(EC 4.2.1.a)によって4-ヒドロキシブチリル-CoAに変換することができる。4-ヒドロキシブチリル-CoAを4-ヒドロキシブチリル-CoAレダクターゼ(アルコール形成)(EC 1.1.1.c)によって1,4-ブタンジオールに変換することができる。代替的に、4-ヒドロキシブチリル-CoAを4-ヒドロキシブチリル-CoAレダクターゼ(又は4-ヒドロキシブタナールデヒドロゲナーゼ)(EC 1.2.1.b)によって4-ヒドロキシブタナールに変換することができる。4-ヒドロキシブタナールを1,4-ブタンジオールデヒドロゲナーゼ(EC 1.1.1.a)によって1,4-ブタンジオールに変換することができる。
Figure 2012501678
Figure 2012501678

Claims (21)

1,4-ブタンジオール(BDO)経路を有する非天然微生物体であって、該微生物体は、BDOを生成するのに十分な量で発現されるBDO経路酵素をコードする少なくとも1つの外因性核酸を含み、該BDO経路酵素が、
（a）アセトアセチル-補酵素A（CoA）の3-ヒドロキシブチリル-CoAへの変換を触媒する3-ヒドロキシブチリル-CoAデヒドロゲナーゼ；（b）3-ヒドロキシブチリル-CoAのクロトノイル-CoAへの変換を触媒する3-ヒドロキシブチリル-CoAデヒドラターゼ；（c）クロトノイル-CoAのビニルアセチル-CoAへの変換を触媒するビニルアセチル-CoAΔ-イソメラーゼ；及び（d）ビニルアセチル-CoAの4-ヒドロキシブチリル-CoAへの変換を触媒する4-ヒドロキシブチリル-CoAデヒドラターゼを含み、
該微生物体が、（e）4-ヒドロキシブチリル-CoAの1,4-ブタンジオールへの変換を触媒する4-ヒドロキシブチリル-CoAレダクターゼ(アルコール形成)；（f）4-ヒドロキシブチリル-CoAの4-ヒドロキシブタナールへの変換を触媒する4-ヒドロキシブチリル-CoAレダクターゼ；及び（g）4-ヒドロキシブタナールの1,4-ブタンジオールへの変換を触媒する1,4-ブタンジオールデヒドロゲナーゼから選択される酵素をコードする少なくとも1つの外因性核酸を更に含む、前記非天然微生物体。
前記BDO経路酵素をコードする３以上の外因性核酸を含む、請求項1記載の非天然微生物体。
前記BDO経路酵素をコードする４以上の外因性核酸を含む、請求項1記載の非天然微生物体。
前記BDO経路酵素をコードする５以上の外因性核酸を含む、請求項1記載の非天然微生物体。
前記BDO経路酵素（a）3-ヒドロキシブチリル-CoAデヒドロゲナーゼ、（b）3-ヒドロキシブチリル-CoAデヒドラターゼ、（c）ビニルアセチル-CoAΔ-イソメラーゼ、及び（d）4-ヒドロキシブチリル-CoAデヒドラターゼ；並びに
（e）4-ヒドロキシブチリル-CoAレダクターゼ(アルコール形成)、又は（f）4-ヒドロキシブチリル-CoAレダクターゼ、及び（g）1,4-ブタンジオールデヒドロゲナーゼ
をコードする外因性核酸を含む、請求項1記載の非天然微生物体。
前記BDO経路酵素が、4-ヒドロキシブチリル-CoAレダクターゼ(アルコール形成)を含む、請求項1〜5のいずれか1項記載の非天然微生物体。
前記BDO経路酵素が、4-ヒドロキシブチリル-CoAレダクターゼ、及び1,4-ブタンジオールデヒドロゲナーゼを含む、請求項1〜5のいずれか1項記載の非天然微生物体。
前記BDO経路酵素が、4-ヒドロキシブチリル-CoAレダクターゼ(アルコール形成)、4-ヒドロキシブチリル-CoAレダクターゼ、及び1,4-ブタンジオールデヒドロゲナーゼを含む、請求項1〜5のいずれか1項記載の非天然微生物体。
前記少なくとも1つの外因性核酸が異種核酸である、請求項1〜8のいずれか1項記載の非天然微生物体。
実質的に嫌気性の培地に存在する、請求項1〜9のいずれか1項記載の非天然微生物体。
請求項1記載の非天然微生物体を、BDOを生成するための条件下で、且つ十分な時間にわたって培養することを含む、BDOを生成するための方法。
前記微生物体が、前記BDO経路酵素をコードする３以上の外因性核酸を含む、請求項11記載の方法。
前記微生物体が、前記BDO経路酵素をコードする４以上の外因性核酸を含む、請求項11記載の方法。
前記微生物体が、前記BDO経路酵素をコードする５以上の外因性核酸を含む、請求項11記載の方法。
前記微生物体が、前記BDO経路酵素（a）3-ヒドロキシブチリル-CoAデヒドロゲナーゼ、（b）3-ヒドロキシブチリル-CoAデヒドラターゼ、（c）ビニルアセチル-CoAΔ-イソメラーゼ、及び（d）4-ヒドロキシブチリル-CoAデヒドラターゼ；並びに
（e）4-ヒドロキシブチリル-CoAレダクターゼ(アルコール形成)、又は（f）4-ヒドロキシブチリル-CoAレダクターゼ、及び（g）1,4-ブタンジオールデヒドロゲナーゼ
をコードする外因性核酸を含む、請求項11記載の方法。
前記BDO経路が、4-ヒドロキシブチリル-CoAレダクターゼ(アルコール形成)を含む、請求項11〜15のいずれか1項記載の方法。
前記BDO経路が、4-ヒドロキシブチリル-CoAレダクターゼ、及び1,4-ブタンジオールデヒドロゲナーゼを含む、請求項11〜15のいずれか1項記載の方法。
前記BDO経路が、4-ヒドロキシブチリル-CoAレダクターゼ(アルコール形成)、4-ヒドロキシブチリル-CoAレダクターゼ、及び1,4-ブタンジオールデヒドロゲナーゼを含む、請求項11〜15のいずれか1項記載の方法。
前記非天然微生物体が実質的に嫌気性の培地に存在する、請求項11〜18のいずれか1項記載の方法。
前記非天然微生物体が、実質的に嫌気性条件下で培養される、請求項11〜18のいずれか1項記載の方法。
前記少なくとも1つの外因性核酸が異種核酸である、請求項11〜20のいずれか1項記載の方法。
JP2011526949A 2008-09-10 2009-09-09 １，４−ブタンジオールの生成のための微生物体 Active JP5912529B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US19171008P 2008-09-10 2008-09-10
US61/191,710 2008-09-10
US19251108P 2008-09-17 2008-09-17
US61/192,511 2008-09-17
PCT/US2009/056415 WO2010030711A2 (en) 2008-09-10 2009-09-09 Microorganisms for the production of 1,4-butanediol

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015133621A Division JP2015221043A (ja) 2008-09-10 2015-07-02 １，４−ブタンジオールの生成のための微生物体

Publications (3)

Publication Number Publication Date
JP2012501678A JP2012501678A (ja) 2012-01-26
JP2012501678A5 true JP2012501678A5 (ja) 2014-11-20
JP5912529B2 JP5912529B2 (ja) 2016-04-27

Family

ID=42005726

Family Applications (5)

Application Number Title Priority Date Filing Date
JP2011526949A Active JP5912529B2 (ja) 2008-09-10 2009-09-09 １，４−ブタンジオールの生成のための微生物体
JP2015133621A Pending JP2015221043A (ja) 2008-09-10 2015-07-02 １，４−ブタンジオールの生成のための微生物体
JP2017122737A Pending JP2017205116A (ja) 2008-09-10 2017-06-23 １，４−ブタンジオールの生成のための微生物体
JP2019135844A Pending JP2020010685A (ja) 2008-09-10 2019-07-24 １，４−ブタンジオールの生成のための微生物体
JP2021136782A Pending JP2021192622A (ja) 2008-09-10 2021-08-25 １，４−ブタンジオールの生成のための微生物体

Family Applications After (4)

Application Number Title Priority Date Filing Date
JP2015133621A Pending JP2015221043A (ja) 2008-09-10 2015-07-02 １，４−ブタンジオールの生成のための微生物体
JP2017122737A Pending JP2017205116A (ja) 2008-09-10 2017-06-23 １，４−ブタンジオールの生成のための微生物体
JP2019135844A Pending JP2020010685A (ja) 2008-09-10 2019-07-24 １，４−ブタンジオールの生成のための微生物体
JP2021136782A Pending JP2021192622A (ja) 2008-09-10 2021-08-25 １，４−ブタンジオールの生成のための微生物体

Country Status (8)

Country Link
US (7) US7858350B2 (ja)
EP (2) EP3514242A3 (ja)
JP (5) JP5912529B2 (ja)
AU (1) AU2009291825B2 (ja)
BR (1) BRPI0918483A2 (ja)
CA (1) CA2735883C (ja)
TW (2) TW201529856A (ja)
WO (1) WO2010030711A2 (ja)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0605890D0 (en) * 2006-03-24 2006-05-03 Bioconversion Technologies Ltd Enhancemeny Of Microbial Ethanol Production
EP2137315B1 (en) 2007-03-16 2014-09-03 Genomatica, Inc. Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors
TWI525195B (zh) 2007-08-10 2016-03-11 奇諾麥提卡公司 合成烯酸及其衍生物之方法
US7947483B2 (en) 2007-08-10 2011-05-24 Genomatica, Inc. Methods and organisms for the growth-coupled production of 1,4-butanediol
EP2245137B1 (en) * 2008-01-22 2017-08-16 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
CA2725549A1 (en) 2008-06-17 2009-12-23 Genomatica, Inc. Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
EP2313491A4 (en) * 2008-07-08 2011-12-07 Opx Biotechnologies Inc METHODS, COMPOSITIONS AND SYSTEMS FOR BIOSYNTHETIC BIOPRODUCTION OF 1,4-BUTANEDIOL
EP3514242A3 (en) 2008-09-10 2019-08-28 Genomatica, Inc. Microrganisms for the production of 1,4-butanediol
WO2010057022A1 (en) * 2008-11-14 2010-05-20 Genomatica, Inc. Microorganisms for the production of methyl ethyl ketone and 2-butanol
CN102307986A (zh) 2008-12-16 2012-01-04 基因组股份公司 用于将合成气和其他碳源转化成有用产品的微生物和方法
EP2424975B1 (en) 2009-04-30 2016-07-06 Genomatica, Inc. Organisms for the production of 1,3-butanediol
JP5964747B2 (ja) 2009-06-04 2016-08-03 ゲノマチカ， インク． １，４−ブタンジオールの生成のための微生物体及び関連する方法
SG176654A1 (en) 2009-06-04 2012-01-30 Genomatica Inc Process of separating components of a fermentation broth
WO2010144746A2 (en) 2009-06-10 2010-12-16 Genomatica, Inc. Microorganisms and methods for carbon-efficient biosynthesis of mek and 2-butanol
WO2011031897A1 (en) * 2009-09-09 2011-03-17 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
US8377666B2 (en) 2009-10-13 2013-02-19 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto
EP2495328B1 (en) * 2009-10-29 2016-03-30 Korea Research Institute of Bioscience and Biotechnology Novel method for producing 3-hydroxypropionic acid from glycerol
WO2011066076A1 (en) 2009-11-25 2011-06-03 Genomatica, Inc. Microorganisms and methods for the coproduction of 1,4-butanediol and gamma-butyrolactone
WO2011071682A1 (en) * 2009-12-10 2011-06-16 Genomatica, Inc. Methods and organisms for converting synthesis gas or other gaseous carbon sources and methanol to 1,3-butanediol
CN102781901B (zh) 2010-02-11 2016-08-03 梅塔玻利克斯公司 用于从经遗传修饰的聚羟基链烷酸酯生物质制备单体组分的方法
US8445244B2 (en) 2010-02-23 2013-05-21 Genomatica, Inc. Methods for increasing product yields
US9200288B2 (en) 2010-04-27 2015-12-01 The Regents Of The University Of California Production of 1,4-butanediol by recombinant microorganisms
KR20190006103A (ko) 2010-05-05 2019-01-16 게노마티카 인코포레이티드 부타디엔을 생합성하기 위한 미생물 및 방법
EP2503003A1 (en) 2011-03-24 2012-09-26 Volker Sieber Synthetic pathway for the production of alcohols or amines
MX2013011139A (es) 2011-04-12 2013-10-30 Procter & Gamble Envases de barrera flexible derivados de recursos renovables.
US20140134691A1 (en) * 2011-05-27 2014-05-15 Novozymes A/S Microorganisms for n-Propanol Production
US9155139B2 (en) 2012-03-09 2015-10-06 Rockwell Automation Technologies, Inc. LED driver circuits and methods
US20150159184A1 (en) * 2012-03-20 2015-06-11 Metabolix, Inc. Genetically Engineered Microorganisms for the Production of Poly-4-Hydroxybutyrate
US9677045B2 (en) * 2012-06-04 2017-06-13 Genomatica, Inc. Microorganisms and methods for production of 4-hydroxybutyrate, 1,4-butanediol and related compounds
KR102023618B1 (ko) 2012-07-27 2019-09-20 삼성전자주식회사 1,4-ｂｄｏ 생성능이 개선된 변이 미생물 및 이를 이용한 1,4-ｂｄｏ의 제조방법
US9493746B2 (en) 2012-07-30 2016-11-15 Samsung Electronics Co., Ltd. Enzyme used in biosynthesis of 1, 4-BDO and screening method of the same
KR20140016654A (ko) 2012-07-30 2014-02-10 삼성전자주식회사 대장균 내에서 1,4-부탄디올의 생합성에 사용되는 효소의 개량과 개선된 유전자를 스크리닝 하는 방법
US9657316B2 (en) 2012-08-27 2017-05-23 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,4-butanediol related thereto
JPWO2014045781A1 (ja) * 2012-09-24 2016-08-18 昭和電工株式会社 ブタンジオール類の製造方法
US9909150B2 (en) 2012-11-05 2018-03-06 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,2-propanediol, n-propanol, 1,3-propanediol, or glycerol related thereto
WO2014080687A1 (ja) * 2012-11-26 2014-05-30 昭和電工株式会社 １，４－ブタンジオールの製造方法及び微生物
CN104822831A (zh) * 2012-12-05 2015-08-05 昭和电工株式会社 1,4丁二醇的制造方法、微生物以及基因
MY181137A (en) 2013-03-15 2020-12-18 Genomatica Inc Process and systems for obtaining 1,4-butanediol from fermentation broths
MY173430A (en) 2013-04-12 2020-01-24 Toray Industries Process of producing 1,4-butanediol
WO2014176186A2 (en) * 2013-04-22 2014-10-30 William Marsh Rice University Microbial alkanes from c-1 substrate
WO2014210587A1 (en) * 2013-06-29 2014-12-31 The Regents Of The University Of California Recombinant plants and microorganisms having a reverse glyoxylate shunt
KR102113254B1 (ko) 2013-08-23 2020-05-21 삼성전자주식회사 1,4―ｂｄｏ 생산을 위한 유전자 스크리닝 방법
US10035749B2 (en) 2013-09-03 2018-07-31 Myriant Corporation Process for manufacturing acrylic acid, acrylonitrile and 1,4-butanediol from 1,3-propanediol
EP3967747B1 (en) 2013-12-03 2023-11-08 Genomatica, Inc. Microorganisms and methods for improving product yields on methanol using acetyl-coa synthesis
CA3192376A1 (en) 2013-12-27 2015-07-02 Genomatica, Inc. Methods and organisms with increased carbon flux efficiencies
US10059920B2 (en) 2014-01-16 2018-08-28 University Of Delaware Synthetic methylotrophy to liquid fuels and chemicals
EP3132046B1 (en) 2014-04-16 2019-07-24 Novamont S.p.A. Process for the production of 1,4-butanediol
EP4421181A2 (en) 2014-09-18 2024-08-28 Genomatica, Inc. Non-natural microbial organisms with improved energetic efficiency
WO2016066869A1 (es) 2014-10-30 2016-05-06 Abengoa Research, S.L. Catalizador microporoso con encapsulación selectiva de óxidos metálicos útil para producir precursores de butadieno
WO2016066873A1 (es) 2014-10-30 2016-05-06 Abengoa Research, S.L. Óxidos mixtos que comprenden magnesio y boro, y su uso como catalizadores para producir precursores de butadieno
KR101748930B1 (ko) * 2015-02-09 2017-06-20 지에스칼텍스 주식회사 다이올 생산용 재조합 미생물
EP3273782B9 (en) 2015-02-27 2022-07-13 White Dog Labs, Inc. Mixotrophic fermentation method for making acetone, isopropanol, and other bioproducts, and mixtures thereof
BR112019006744A2 (pt) 2016-10-03 2019-06-25 Univ California micro-organismos engenheirados para produção de produtos químicos de commodity e biomassa celular
KR20240033193A (ko) 2017-03-31 2024-03-12 게노마티카 인코포레이티드 알데하이드 데하이드로게나제 변이체 및 사용 방법
CN111601648B (zh) 2017-11-27 2022-08-02 诺瓦蒙特股份公司 用于生产来自可再生资源的1，4-丁二醇的方法和由其获得的聚酯
US20210079334A1 (en) 2018-01-30 2021-03-18 Genomatica, Inc. Fermentation systems and methods with substantially uniform volumetric uptake rate of a reactive gaseous component
EP3814515A2 (en) 2018-06-26 2021-05-05 Genomatica, Inc. Engineered microorganisms with g3p---> 3pg enzyme and/or fructose-1,6-bisphosphatase including those having synthetic or enhanced methylotrophy
US11634692B2 (en) 2018-09-26 2023-04-25 Genomatica, Inc. Aldehyde dehydrogenase variants and methods of using same
JP2023519172A (ja) * 2020-03-13 2023-05-10 ディーエスエム アイピー アセッツ ビー．ブイ． 胃腸微生物代謝経路及び代謝物を調節する方法
IT202000013243A1 (it) 2020-06-04 2021-12-04 Novamont Spa Processo per la purificazione di una miscela di dioli
FR3125046B1 (fr) * 2021-07-09 2024-05-10 Snf Sa Procédé d’obtention de monomere N-vinylpyrrolidone biosourcé
IT202100030572A1 (it) 2021-12-02 2023-06-02 Novamont Spa 1,3-butandiolo purificato da una miscela di dioli
WO2023126937A1 (en) * 2021-12-29 2023-07-06 B. G. Negev Technologies And Applications Ltd., At Ben-Gurion University Selective separation of ammonium and lactate from cell culture media
CN116083329A (zh) * 2022-09-26 2023-05-09 北京绿色康成生物技术有限公司 发酵生产γ-丁内酯或1,4-丁二醇的方法

Family Cites Families (158)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1230276A (ja) 1968-12-09 1971-04-28
JPS4831084B1 (ja) 1970-09-04 1973-09-26
GB1344557A (en) 1972-06-23 1974-01-23 Mitsubishi Petrochemical Co Process for preparing 1,4-butanediol
DE2455617C3 (de) 1974-11-23 1982-03-18 Basf Ag, 6700 Ludwigshafen Verfahren zur Herstellung von Butandiol und/oder Tetrahydrofuran über die Zwischenstufe des γ-Butyrolactons
DE2501499A1 (de) 1975-01-16 1976-07-22 Hoechst Ag Verfahren zur herstellung von butandiol-(1.4)
GB1583517A (en) 1977-05-04 1981-01-28 Jackson J F Solid bowl decanter centrifuges of the scroll discharge type
DE2842575A1 (de) * 1977-10-04 1979-04-12 Broadbent & Sons Ltd Thomas Vollmantel-abklaerzentrifuge
JPS575693A (en) * 1980-06-13 1982-01-12 Ajinomoto Co Inc Production of l-arginine through fermentation process
US4301077A (en) 1980-12-22 1981-11-17 Standard Oil Company Process for the manufacture of 1-4-butanediol and tetrahydrofuran
IT1190783B (it) 1981-04-29 1988-02-24 Davy Mckee Oil & Chem Processo per l'idrogenolisi di esteri di acidi carbossilici
US4652685A (en) 1985-11-15 1987-03-24 General Electric Company Hydrogenation of lactones to glycols
JPS62285779A (ja) * 1986-06-04 1987-12-11 Chiyoda Chem Eng & Constr Co Ltd １，４−ブタンジオ−ル産生バチルス属細菌及びそれを用いる１，４−ブタンジオ−ルの製造方法
US5245023A (en) * 1987-06-29 1993-09-14 Massachusetts Institute Of Technology Method for producing novel polyester biopolymers
US5250430A (en) * 1987-06-29 1993-10-05 Massachusetts Institute Of Technology Polyhydroxyalkanoate polymerase
US4876331A (en) * 1987-08-18 1989-10-24 Mitsubishi Kasei Corporation Copolyester and process for producing the same
DE68928956T2 (de) * 1988-04-27 1999-07-29 Daicel Chemical Industries, Ltd., Sakai, Osaka Verfahren zur Herstellung von optisch aktivem 1,3-Butanediol
US4925608A (en) 1988-09-27 1990-05-15 Norton Company Joining of SiC parts by polishing and hipping
DE3878564T2 (de) 1988-12-12 1993-08-19 Unilever Nv Verfahren zur mikrobiologischen herstellung von 1,3-propandiol aus glycerin.
US5371002A (en) 1989-06-07 1994-12-06 James Madison University Method of production of poly-beta-hydroxyalkanoate copolymers
ES2125222T3 (es) 1989-07-10 1999-03-01 Massachusetts Inst Technology Procedimiento de produccion de biopolimeros a base de poliesteres.
GB9011777D0 (en) * 1990-05-25 1990-07-18 Ici Plc Hv/hb copolymer production
US5475086A (en) 1990-09-21 1995-12-12 The Regents Of The University Of California Cloned glutamic acid decarboxylase peptides
US6682906B1 (en) * 1990-09-21 2004-01-27 The Regents Of The University Of California Cloned glutamic acid decarboxylase
US5674978A (en) 1990-09-21 1997-10-07 The Regents Of The University Of California Peptides derived from glutamic acid decarboxylase
US5998366A (en) 1990-09-21 1999-12-07 The Regents Of The University Of California Method for ameliorating glutamic acid decarboxylase associated autoimmune disorders
GB9108756D0 (en) * 1991-04-24 1991-06-12 Ici Plc Production of polyalkanoate in plants
US5286842A (en) * 1991-07-01 1994-02-15 Mitsubishi Kasei Corporation Process for producing a biodegradable polymer
GB9115245D0 (en) * 1991-07-16 1991-08-28 Ici Plc Production of polyalkanoate
JP3696233B2 (ja) * 1991-07-19 2005-09-14 ミシガン、ステート、ユニバーシティー ポリヒドロキシアルカノエートを生産するトランスジェニック植物
US5610041A (en) * 1991-07-19 1997-03-11 Board Of Trustees Operating Michigan State University Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants
FR2680178B1 (fr) * 1991-08-07 1994-10-14 Ajinomoto Kk Procede pour produire l'acide l-glutamique par fermentation.
JP2777757B2 (ja) * 1991-09-17 1998-07-23 鐘淵化学工業株式会社 共重合体およびその製造方法
DE4220759A1 (de) 1992-06-24 1994-01-05 Inst Genbiologische Forschung DNA-Sequenzen für Oligosaccharid-Transporter, Plasmide, Bakterien und Pflanzen enthaltend einen Transporter sowie Verfahren zur Herstellung und Transformation von Hefestämmen zur Identifikation des Transporteers
GB9223332D0 (en) 1992-11-06 1992-12-23 Ici Plc Production of polyhydroxyalkanoate in plants
AU5676394A (en) 1992-11-20 1994-06-22 Agracetus, Inc. Transgenic cotton plants producing heterologous bioplastic
JP3263710B2 (ja) * 1992-12-11 2002-03-11 高砂香料工業株式会社 生分解性光学活性ポリマー及びその製造方法
JP3241505B2 (ja) * 1993-08-11 2001-12-25 高砂香料工業株式会社 生分解性光学活性コポリマー及びその製造方法
RU2182173C2 (ru) 1993-10-28 2002-05-10 Адзиномото Ко., Инк. Способ получения l-аминокислоты
GB9414506D0 (en) * 1994-07-18 1994-09-07 Zeneca Ltd Improved production of polyhydroxyalkanoate
DE4433134A1 (de) * 1994-09-16 1996-03-21 Buck Chem Tech Werke Verfahren zur Herstellung von Polyhydroxyfettsäuren sowie rekombinanter Bakterienstämme zur Durchführung des Verfahrens
US5563239A (en) * 1994-11-09 1996-10-08 Eastman Chemical Company Composition and process for the production of poly(3-hydroxyalkanoates)
US5478952A (en) 1995-03-03 1995-12-26 E. I. Du Pont De Nemours And Company Ru,Re/carbon catalyst for hydrogenation in aqueous solution
US5747311A (en) * 1995-08-22 1998-05-05 Microgen Corporation Process for chemical modification of reactants by microbes
US5869301A (en) * 1995-11-02 1999-02-09 Lockhead Martin Energy Research Corporation Method for the production of dicarboxylic acids
US5770435A (en) * 1995-11-02 1998-06-23 University Of Chicago Mutant E. coli strain with increased succinic acid production
DE19543635A1 (de) * 1995-11-23 1997-05-28 Hp Chemie Pelzer Res & Dev Verbundwerkstoffe aus Polyhydroxyfettsäuren und Fasermaterialien
US5849894A (en) 1995-11-29 1998-12-15 Monsanto Company Rhodospirillum rubrum poly-β-hydroxyalkanoate synthase
IL125910A0 (en) * 1996-02-27 1999-04-11 Univ Michigan State Cloning and expression of the gene encoding thermoanaerobacter ethanolicus 39e secondary-alcohol dehydrogenase and enzyme biochemical characterization
US5958745A (en) * 1996-03-13 1999-09-28 Monsanto Company Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants
US5959179A (en) * 1996-03-13 1999-09-28 Monsanto Company Method for transforming soybeans
US6091002A (en) * 1996-03-13 2000-07-18 Monsanto Company Polyhydroxyalkanoates of narrow molecular weight distribution prepared in transgenic plants
US5750848A (en) * 1996-08-13 1998-05-12 Monsanto Company DNA sequence useful for the production of polyhydroxyalkanoates
US6730503B1 (en) * 1996-09-19 2004-05-04 Roche Vitamins Inc. Alcohol/aldehyde dehydrogenase
JPH10166664A (ja) * 1996-12-12 1998-06-23 Casio Electron Mfg Co Ltd カラー印刷装置
WO1998036078A1 (en) * 1997-02-13 1998-08-20 James Madison University Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units
US6759219B2 (en) * 1997-03-03 2004-07-06 Metabolix, Inc. Methods for the biosynthesis of polyesters
US6156852A (en) * 1997-04-21 2000-12-05 Monsanto Company Hydroxy-terminated polyhydroxyalkanoates
WO1998048028A1 (en) * 1997-04-21 1998-10-29 Monsanto Company Hydroxy-terminated polyhydroxyalkanoates
KR19990013007A (ko) 1997-07-31 1999-02-25 박원훈 형질전환된 대장균 ｓｓ373(ｋｃｔｃ 8818ｐ)과 이를 이용한숙신산의 생산방법
ATE323152T1 (de) * 1997-09-19 2006-04-15 Metabolix Inc Biologische systeme zur herstellung von polyhydroxyalkanoate polymeren die 4-hydroxysäure enthalten
US6228579B1 (en) 1997-11-14 2001-05-08 San Diego State University Foundation Method for identifying microbial proliferation genes
US6280986B1 (en) * 1997-12-01 2001-08-28 The United States Of America As Represented By The Secretary Of Agriculture Stabilization of pet operon plasmids and ethanol production in bacterial strains lacking lactate dehydrogenase and pyruvate formate lyase activities
US6159738A (en) 1998-04-28 2000-12-12 University Of Chicago Method for construction of bacterial strains with increased succinic acid production
WO2000004163A1 (en) 1998-07-15 2000-01-27 E.I. Du Pont De Nemours And Company Tetrahydrofolate metabolism enzymes
ATE385521T1 (de) * 1998-08-04 2008-02-15 Metabolix Inc Herstellung von polyhydroxyalkanoaten aus polyolen
MXPA01001750A (es) * 1998-08-18 2002-04-08 Metabolix Inc Productos microbianos transgenicos de polihidroxialcanoato.
US6835820B2 (en) 1999-01-07 2004-12-28 The University Of Massachusetts Polyhydroxyalkanoate biosynthesis associated proteins and coding region in bacillus megaterium
AU3482200A (en) * 1999-02-02 2000-08-25 Bernhard Palsson Methods for identifying drug targets based on genomic sequence data
US6686310B1 (en) * 1999-02-09 2004-02-03 E. I. Du Pont De Nemours And Company High surface area sol-gel route prepared hydrogenation catalysts
AU3516100A (en) * 1999-03-05 2000-09-21 Monsanto Technology Llc Multigene expression vectors for the biosynthesis of products via multienzyme biological pathways
CA2368953A1 (en) * 1999-04-09 2000-10-19 University Of Guelph Proteins related to gaba metabolism
KR100329019B1 (ko) * 1999-04-13 2002-03-18 윤덕용 유기산의 고효율 생산방법
ATE335823T1 (de) 1999-08-18 2006-09-15 Du Pont Verfahren zur biologischen herstellung von 1,3- propandiol
US6361983B1 (en) 1999-09-30 2002-03-26 E. I. Du Pont De Nemours And Company Process for the isolation of 1,3-propanediol from fermentation broth
US6897055B2 (en) * 1999-11-25 2005-05-24 Degussa Ag Nucleotide sequences coding for the genes sucC and sucD
DE19956686A1 (de) * 1999-11-25 2001-05-31 Degussa Neue für die Gene sucC und sucD codierende Nukleotidsequenzen
ES2281412T3 (es) * 2000-04-21 2007-10-01 Mitsubishi Rayon Co., Ltd. Composicion de resina epoxi y prepreg fabricado con la composicion de resina epoxi.
KR20040014389A (ko) 2000-07-21 2004-02-14 메타볼릭스 인코포레이티드 폴리올로부터 폴리하이드록시알칸노에이트를 제조하는 방법
WO2002016627A2 (en) 2000-08-18 2002-02-28 Tepha, Inc. Sulfur containing polyhydroxyalkanoate compositions and method of production
US6916637B2 (en) * 2000-09-30 2005-07-12 Degussa Ag Fermentation process for the preparation of L-amino acids using strains of the family Enterobacteriaceae
AU2002219818B2 (en) * 2000-11-20 2007-08-16 Cargill, Incorporated 3-hydroxypropionic acid and other organic compounds
EP1354955A4 (en) * 2000-12-28 2005-01-05 Toyota Motor Co Ltd PROCESS FOR PREPARING PRENYL ALCOHOL
AU2002239855B2 (en) 2001-01-10 2006-11-23 The Penn State Research Foundation Method and system for modeling cellular metabolism
US7127379B2 (en) 2001-01-31 2006-10-24 The Regents Of The University Of California Method for the evolutionary design of biochemical reaction networks
CA2439260C (en) * 2001-03-01 2012-10-23 The Regents Of The University Of California Models and methods for determining systemic properties of regulated reaction networks
US7052883B2 (en) * 2001-04-03 2006-05-30 Degussa Ag Process for the production of L-amino acids using strains of the family Enterobacteriaceae that contain an attenuated fruR gene
DE60225288T2 (de) 2001-07-18 2009-03-26 Evonik Degussa Gmbh Verfahren zur herstellung von l-aminosäuren unter verwendung von stämmen aus der familie der enterobacteriaceae die ein attenuiertes ugpb-gen enthalten
US20090158452A1 (en) 2001-12-04 2009-06-18 Johnson Richard G Transgenic plants with enhanced agronomic traits
US20090100536A1 (en) 2001-12-04 2009-04-16 Monsanto Company Transgenic plants with enhanced agronomic traits
CN1217001C (zh) 2002-01-04 2005-08-31 清华大学 一种生产d－(－)－3－羟基丁酸的方法
CA2473716A1 (en) 2002-01-18 2003-07-31 Cargill, Incorporated Alanine 2,3- aminomutase
US7314974B2 (en) 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
US20030224363A1 (en) 2002-03-19 2003-12-04 Park Sung M. Compositions and methods for modeling bacillus subtilis metabolism
WO2003082214A2 (en) 2002-03-29 2003-10-09 Genomatica, Inc. Human metabolic models and methods
EP1510583A1 (en) 2002-05-10 2005-03-02 Kyowa Hakko Kogyo Co., Ltd. Process for producing mevalonic acid
US7856317B2 (en) 2002-06-14 2010-12-21 Genomatica, Inc. Systems and methods for constructing genomic-based phenotypic models
WO2004018621A2 (en) 2002-07-10 2004-03-04 The Penn State Research Foundation Method for determining gene knockout strategies
WO2004007688A2 (en) * 2002-07-15 2004-01-22 Kosan Biosciences, Inc. Metabolic pathways for starter units in polyketide biosynthesis
JP4578240B2 (ja) * 2002-08-09 2010-11-10 コデクシス， インコーポレイテッド ４−置換３−ヒドロキシ酪酸誘導体の生成のための酵素的プロセス
JP4628103B2 (ja) * 2002-09-12 2011-02-09 メタボリックス，インコーポレイテッド 補酵素ａ依存性アルデヒドデヒドロゲナーゼ経路によるポリヒドロキシアルカノエート産生
AU2003299104A1 (en) 2002-09-27 2004-04-19 Dsm Ip Assets B.V. Aldehyde dehydrogenase gene
EP1552472A4 (en) * 2002-10-15 2008-02-20 Univ California METHODS AND SYSTEMS FOR IDENTIFYING FUNCTIONAL REACTION PATHWAYS
US20040152159A1 (en) * 2002-11-06 2004-08-05 Causey Thomas B. Materials and methods for the efficient production of acetate and other products
KR20060071397A (ko) 2003-08-11 2006-06-26 코덱시스, 인코포레이티드 4-치환된 3-히드록시부티르산 유도체 및 이웃자리 시아노,히드록시 치환된 카르복실산 에스테르의 효소적 제조 방법
US7927859B2 (en) * 2003-08-22 2011-04-19 Rice University High molar succinate yield bacteria by increasing the intracellular NADH availability
WO2005026338A1 (en) 2003-09-18 2005-03-24 Ciba Specialty Chemicals Holding Inc. Alcohol dehydrogenases with increased solvent and temperature stability
WO2005052135A1 (en) * 2003-11-27 2005-06-09 Korea Advanced Institute Of Science And Technology Novel rumen bacteria variants and process for preparing succinic acid employing the same
FR2864967B1 (fr) * 2004-01-12 2006-05-19 Metabolic Explorer Sa Microorganisme evolue pour la production de 1,2-propanediol
DE102004031177A1 (de) * 2004-06-29 2006-01-19 Henkel Kgaa Neue Geruchsstoffe bildende Genprodukte von Bacillus licheniformis und darauf aufbauende verbesserte biotechnologische Produktionsverfahren
KR100656590B1 (ko) 2004-07-30 2006-12-11 한국과학기술원 박테리아 변이균주 및 이를 이용한 숙신산 및 아미노산의제조방법
US7601858B2 (en) 2004-08-17 2009-10-13 Gs Cleantech Corporation Method of processing ethanol byproducts and related subsystems
BRPI0514734B1 (pt) * 2004-08-27 2018-02-06 Rice University VARIEDADE BACTERIANA DE E. Coli MODIFICADA
GB0419424D0 (en) 2004-09-02 2004-10-06 Viragen Scotland Ltd Transgene optimisation
WO2006028063A1 (ja) * 2004-09-09 2006-03-16 Research Institute Of Innovative Technology For The Earth プロモーター機能を有するｄｎａ断片
WO2006069174A2 (en) * 2004-12-22 2006-06-29 Rice University Simultaneous anaerobic production of isoamyl acetate and succinic acid
KR100727054B1 (ko) 2005-08-19 2007-06-12 한국과학기술원 푸마레이트 하이드라타제 ｃ를 코딩하는 유전자로 형질전환된 재조합 미생물 및 이를 이용한 숙신산의 제조방법
KR100679638B1 (ko) 2005-08-19 2007-02-06 한국과학기술원 포메이트 디하이드로게나제 ｄ 또는 ｅ를 코딩하는 유전자로 형질전환된 미생물 및 이를 이용한 숙신산의 제조방법
KR100676160B1 (ko) 2005-08-19 2007-02-01 한국과학기술원 말릭효소를 코딩하는 유전자로 형질전환된 재조합 미생물 및 이를 이용한 숙신산의 제조방법
EP1937821A4 (en) 2005-09-09 2009-11-11 Genomatica Inc PROCESS AND ORGANISMS FOR GROWTH-COUPLED PRODUCTION OF SUCCINATE
US9297028B2 (en) * 2005-09-29 2016-03-29 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
US20080274526A1 (en) 2007-05-02 2008-11-06 Bramucci Michael G Method for the production of isobutanol
KR20070096348A (ko) * 2006-03-23 2007-10-02 주식회사 엘지화학 1,4―ｂｕｔａｎｅｄｉｏｌ〔１，４―ＢＤＯ〕생성능을가지는 변이체 및 이를 이용한 １，４―ＢＤＯ의 제조방법
NZ600540A (en) 2006-05-01 2013-12-20 Univ Florida Ethanol production in non-recombinant hosts
US8206970B2 (en) 2006-05-02 2012-06-26 Butamax(Tm) Advanced Biofuels Llc Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase
DE102006025821A1 (de) 2006-06-02 2007-12-06 Degussa Gmbh Ein Enzym zur Herstellung von Mehylmalonatsemialdehyd oder Malonatsemialdehyd
CN101528918B (zh) 2006-08-30 2012-09-26 诺沃奇梅兹A/S 生产3-羟基丙酸的β-丙氨酸/α-酮戊二酸氨基转移酶
US8017364B2 (en) * 2006-12-12 2011-09-13 Butamax(Tm) Advanced Biofuels Llc Solvent tolerant microorganisms
EP2137315B1 (en) * 2007-03-16 2014-09-03 Genomatica, Inc. Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors
EP2570481B1 (en) 2007-03-20 2014-11-26 University of Florida Research Foundation, Inc. Materials and methods for efficient succinate and malate production
CN101680007A (zh) 2007-04-18 2010-03-24 纳幕尔杜邦公司 使用高活性酮醇酸还原异构酶来发酵生产异丁醇
WO2008131286A1 (en) 2007-04-18 2008-10-30 Gevo, Inc. Engineered microorganisms for producing isopropanol
US8426174B2 (en) 2007-05-02 2013-04-23 Butamax(Tm) Advanced Biofuels Llc Method for the production of 2-butanol
US8426173B2 (en) 2007-05-02 2013-04-23 Butamax (Tm) Advanced Biofuels Llc Method for the production of 1-butanol
EP2158323A4 (en) 2007-05-18 2011-06-22 Microbia Prec Engineering Inc PRODUCTION OF ORGANIC ACID BY PILZ CELLS
EP2155885A4 (en) 2007-05-18 2010-12-01 Microbia Prec Engineering Inc ACIDIC ACID PRODUCTION IN RECOMBINANT YEAST
US20090023182A1 (en) 2007-07-18 2009-01-22 Schilling Christophe H Complementary metabolizing organisms and methods of making same
US7947483B2 (en) 2007-08-10 2011-05-24 Genomatica, Inc. Methods and organisms for the growth-coupled production of 1,4-butanediol
KR101042242B1 (ko) 2007-09-07 2011-06-17 한국과학기술원 1,4-부탄디올 생성능을 가지는 변이체 및 이를 이용한1,4-부탄디올의 제조방법
BRPI0817545A2 (pt) 2007-10-12 2014-11-25 Univ California Microorganismo recombinante, métodos para produzir um microorganismo recombinante e para produzir isopropanol, vetor recombinante, e, célula hospedeira recombinante.
EP2245137B1 (en) 2008-01-22 2017-08-16 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
WO2009103026A1 (en) 2008-02-15 2009-08-20 Gevo, Inc. Engineered microorganisms for producing isopropanol
US20090246842A1 (en) 2008-02-15 2009-10-01 Gevo, Inc. Engineered microorganisms for producing propanol
EP2285948B1 (en) 2008-03-03 2014-01-08 Joule Unlimited Technologies, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
EP2270135A4 (en) 2008-04-25 2013-04-10 Res Inst Innovative Tech Earth GENETICALLY MODIFIED CORYNEFORME BACTERIA FOR THE MANUFACTURE OF ISOPROPANOL
CA2722680A1 (en) 2008-05-01 2009-11-05 Genomatica, Inc. Microorganisms for the production of methacrylic acid
EP2313491A4 (en) 2008-07-08 2011-12-07 Opx Biotechnologies Inc METHODS, COMPOSITIONS AND SYSTEMS FOR BIOSYNTHETIC BIOPRODUCTION OF 1,4-BUTANEDIOL
EP3514242A3 (en) 2008-09-10 2019-08-28 Genomatica, Inc. Microrganisms for the production of 1,4-butanediol
US8357826B2 (en) * 2008-10-16 2013-01-22 Karl Kharas Methods and apparatus for synthesis of alcohols from syngas
CN102307986A (zh) 2008-12-16 2012-01-04 基因组股份公司 用于将合成气和其他碳源转化成有用产品的微生物和方法
US20110014669A1 (en) 2009-01-23 2011-01-20 Microbia, Inc. Production of 1,4 Butanediol in a Microorganism
SG176654A1 (en) 2009-06-04 2012-01-30 Genomatica Inc Process of separating components of a fermentation broth
JP5964747B2 (ja) 2009-06-04 2016-08-03 ゲノマチカ， インク． １，４−ブタンジオールの生成のための微生物体及び関連する方法
WO2011031897A1 (en) 2009-09-09 2011-03-17 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
US8377666B2 (en) 2009-10-13 2013-02-19 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto
WO2011066076A1 (en) 2009-11-25 2011-06-03 Genomatica, Inc. Microorganisms and methods for the coproduction of 1,4-butanediol and gamma-butyrolactone
WO2011071682A1 (en) 2009-12-10 2011-06-16 Genomatica, Inc. Methods and organisms for converting synthesis gas or other gaseous carbon sources and methanol to 1,3-butanediol
US8048661B2 (en) 2010-02-23 2011-11-01 Genomatica, Inc. Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes
US8445244B2 (en) 2010-02-23 2013-05-21 Genomatica, Inc. Methods for increasing product yields

Similar Documents

Publication Publication Date Title
JP2012501678A5 (ja)
JP6905518B2 (ja) エネルギー発生発酵経路を含む遺伝子操作細菌
JP2011519561A5 (ja)
CN103502435B (zh) 重组微生物及其用途
JP2014504508A5 (ja)
US10246726B2 (en) Photosynthetic production of 3-hydroxybutyrate from carbon dioxide
JP2013507145A5 (ja)
Yu et al. Metabolic engineering of Clostridium tyrobutyricum for n-butanol production: effects of CoA transferase
EA201491056A8 (ru) Штаммы дрожжей, сконструированные для получения этанола из уксусной кислоты и глицерина
WO2013028519A4 (en) Microorganisms and methods for producing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol and related alcohols
TR201814888T4 (en) 1,4-METHODS AND ORGANISMS FOR GROWTH PRODUCTION OF BANDANDIOL
CN105745327A (zh) 由可再生资源生产化合物的高产路线
JP2013504326A5 (ja)
CN104508136A (zh) 重组微生物及其用途
CA2797046A1 (en) Microorganisms and methods for the biosynthesis of propylene
JP2015517824A (ja) 生合成経路、組み換え細胞、及び方法
WO2013172928A1 (en) Photosynthetic production of 3-hydroxybutyrate from carbon dioxide
JP2010517562A (ja) ブチリル−ＣｏＡを中間体として酵母を用いてブタノールを製造する方法
WO2015191972A2 (en) Omega-carboxylated carboxylic acids and derivities
US9932609B2 (en) Recombinant microorganism having enhanced 1,3-propanediol producing ability and method for producing 1,3-propanediol using the same
CN104254609B (zh) 正丁醛的微生物生产
CN102808002A (zh) 一种生物法合成甲基乙偶姻及其衍生物的重组细胞和方法
Xiao et al. 3-Methyl-1-butanol biosynthesis in an engineered Corynebacterium glutamicum
CN105705650A (zh) 用于生物合成异丁烯酸盐的方法
US9434963B2 (en) Process for butanol production