HRP960245A2 - Dna sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are tolerant to certain herbicides - Google Patents

Dna sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are tolerant to certain herbicides Download PDF

Info

Publication number
HRP960245A2
HRP960245A2 HR9605944A HRP960245A HRP960245A2 HR P960245 A2 HRP960245 A2 HR P960245A2 HR 9605944 A HR9605944 A HR 9605944A HR P960245 A HRP960245 A HR P960245A HR P960245 A2 HRP960245 A2 HR P960245A2
Authority
HR
Croatia
Prior art keywords
gene
sequence
hppd
plant
promoter
Prior art date
Application number
HR9605944A
Other languages
English (en)
Inventor
Alain Sailland
Anne Rolland
Michel Matringe
Ken Pallett
Original Assignee
Rhone Poulenc Agrochimie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27253066&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HRP960245(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from FR9506800A external-priority patent/FR2734840B1/fr
Priority claimed from FR9513570A external-priority patent/FR2734841B1/fr
Application filed by Rhone Poulenc Agrochimie filed Critical Rhone Poulenc Agrochimie
Publication of HRP960245A2 publication Critical patent/HRP960245A2/hr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Description

Ovaj izum odnosi se na gen hidroksifenilpiruvat dioksigenaze (HPPD), kimerni gen koji uključuje taj gen kao kodnu sekvencu i njegovu upotrebu za dobivanje biljaka otpornih prema određenim herbicidima.
Bili su obznanjeni neki herbicidi, kao izoksazoli posebno opisani u francuskim patentnim prijavama 95 06800 i 95 13570 i naročito izoksaflutol, selektivan herbicid za kukuruz, diketonitrili, kao oni opisani u europskim patentnim prijavama 0 496 630, 0 496 631, i posebno 2-cijano-3-ciklopropil-1-(2-SO2CH3-4-CF3-f enil) propan-1, 3-dion i 2-cijano- 3- ciklopropil-1-(2-SO2CH3-4-2, 3-Cl2-fenil)-propan-1,3-dion, triketoni opisani u. europskim patentnim prijavama 0 625 5.05 i 0 625 508, posebno sulkotrion. Međutim, gen otpornosti prema takovim herbicidima nije bio opisan.
Hidroksifenilpiruvat dioksigenaza je enzim koji katalizira reakciju pretvorbe parahidroksifenilpiruvata u homogenizat.
Dodatno, bila je opisana amino-kiselinska sekvenca hidroksifenilpiruvat dioksigenaze iz Pseudomonas sp. P.J. 874, ali bez opisa njene uloge u otpornosti biljaka preme herbicidima (Ruetchi et al., : Eur. J. Biochem. 205, 459-466, 1992). Taj dokument ne daje opis gena koji kodira taj protein.
Sada je otkrivena sekvenca gena tog tipa i da takav gen, jednom ugrađen u stanice biljke, može proizvesti nad-ekspresiju ili aktivaciju HPPD u biljkama dajući im dragocjenu otpornost prema nekim novim herbicidima, kao što su oni iz skupine izoksazola ili triketona.
Predmet ovog izuma je izolacija DNA sekvence gena nehumanog podrijetla ne-morske bakterije, ili alternativno od biljnog gena, ili sekvence koja se može hibridizirati s tom sekvencom, čija značajka je ekspresija hidroksifenil-piruvat dioksigenaze (HPPD).
Potanje, ova sekvenca može biti bakterijskog podrijetla, kao naročito gen Pseudomonas ili alternativnog biljnog podrijetla, kao naročito od biljaka jednosupnica ili dvosupnica, naročito od Arabidopsis ili Umbelliferae, kao što je, na primjer, mrkva (Daucus carotta} . Može biti nativna ili divlja ili eventualno mutirana, dok istovremeno osnovno zadržava svojstvo herbicidne otpornosti prema HPPD inhibitorima, kao što su herbicidi iz skupine izoksazola ili triketona.
Izum se također odnosi na postupak izolacije gornjeg gena, naznačen time da:
- nekoliko oligonukleotida iz amino-kiselinske sekvence HPPD biraju se su kao prajmeri,
- počevši od tih prajmera, povećani fragmenati sintetiziraju se su pomoću PCR,
- gen se izolira tvorbom i zaklanjanjem genomne banke i
- - gen se klonira.
Ponajprije, upotrijebljeni su prajmeri iz HPPD sekvence bakterije roda Psudomonas. S naročitom prednošću oni su iz Pseudomonas fluorescens.
Izum se također odnosi na upotrebu gena koji kodira HPPD u procesu tranformacije biljaka, kao markera gena ili kao kodna sekvenca koja omogućuje da se na biljku prenese otpornost prema nekim herbicidima. Također se može upotrijebiti i zajedno s drugim genima markerima i/ili kodnim sekvencama za postizanje agronomskog svojstva.
Kodni gen može biti bilo kojeg podrijetla, nativan ili divlji ili eventualno mutiran, dok istovemeno, osnovno, zadržava svojstva herbicidne otpornosti prema HPPD inhibitorima, kao herbicidima iz skupine izoksazola ili triketona. Kao kodna sekvenca naročito se može upotrijebiti ona gore opisana.
Transformacija biljnih stanica može se postići na bilo koji prikladan, poznati način. Niz metoda sastoji se u bombardiranju stanica ili protoplasta sa česticama na koje su povezane DNA sekvence.
Drugi niiz metoda sastoji se u upotrebi kimernog gena ugrađenog u Ti plazmid Agrobacetrium tumefaciens ili Ri plazmid Agrobacterium rhizogenes kao sredstva za transfer u biljku.
Predmet predloženog izuma je također kimerni gen koji u smjeru transkripcije uključuje najmanje jednu sekvencu koja regulira promotor, heterolognu kodnu sekvencu ekspresije hidroksifenilpiruvat dioksigenaze i najmanje jedan terminator ili sekvencu koja regulira poliadenilaciju.
Upotrijebljena sekvenca, koja regulira promotor, može biti bilo koja promotorska sekvenca gena koji se prirodno umnažava u biljkama, naročito promotor bakterijskog, virusnog ili biljnog podrijetla, kao što je, na primjer, onaj od gena male podjedinice ribulozne biskarboksilaze (RiBisCo) ili onaj od gena α-tubulina (europska patentna prikava EP br, 0 652 286), ili alternativno gen biljnog virusa kao, na primjer, onaj od mosaic virusa cvjetače (e. cauliflower mosaic virus, CAMV 19S ili 35S), ali može se upotrijebiti bilo koji prikladan promotor.
Ponajprije, pribjeglo se je prornotorkoj regulacijskoj sekvenci koja potiče nadekspresiju kodne sekvence, kao što je, na primjer, ona koja uključuje najmanje jedan promotor histona, kao što je ona opisana u europskoj patentnoj prijavi EP 0 507 698.
U skladu s izumom jednako se mogu uporijebiti, zajedno s prornotorskom regulacijskom sekvencom, i druge regulacijske sekvence koje su smještene između promotora i kodne sekvence, kao što je “stimulator” transkripcijskih aktivatora, kao što je, na primjer, aktivator translacije duhanskog ureznog virusa (e. tobacco etch virus, TEV) opisan u patentnom spisu WO 87/07644, ili tranzitni peptidi, jednostruki ili dvostruki, i u tom slučaju eventualno odvojeni s intermedi jamom sekvencom, koji dakle u smjeru transkripcije uključuju sekvencu koja kodira tranzitni peptid biljnog gena koji kodira gen lokalizacije plastida, dio sekvence N-terminalnog zrelog dijela biljnog gena koji kodira plastidni lokalizacijski enzim, zatim sekvencu koja kodira za drugi tranzit peptida biljnog gena koji kodira enzim lokalizacije plastida, stvoren pomoću dijela sekvence N-terminalnog zrelog dijela biljnog gena koji kodira enzim lokalizacije plastida, kao što je opisano u europskoj patentnoj prijavi br. 0 508 909.
Kao terminator, ili sekvenca za regulaciju poliadenilacije može se upotrijebiti bilo koja odgovarajuća sekvenca bakterijskog podrijetla, kao što je na primjer nos terminator od Agrobacterium tumefaciens, ili čak biljnog podrijetla, kao što je, na primjer, terminator histona, kao što je onaj opisan u europskoj patentnoj prijavi EP br. 0 633 317.
Predmet ovog izuma su također biljne stanice, biljaka jednosupnica ili dvosupnica, naročito od žitarica, transformirane u skladu s jednim od gore opisanih procesa i koje u svom genomu sadrže učinkovitu količinu gena ekspresije hidroksifenilpiruvat dioksigenaze (HPPD). Također je bilo opaženo da transformirane biljke ovog tipa imaju signifikatnu otpornost prema nekim novim herbicidima kao što su izoksazoli posebno opisani u francuskim patentnim prijavama 95 06800 i 95 13570 i posebno 4-[4-CF3-2- (metilsulfonil)benzoilj-5-ciklopropilizoksazol, i naročito izoksaflutol, selektivni herbicid za kukuruz, diketonitrili, kao oni opisani u europskim patentnim prijavama 0 496 630, 0 496 631, i naročito 2-cijano-3-ciklopropil-1-(2-SO2CH3-4-CF3-fenil)propan-1, 3-dion i 2-cijano-3-ciklopropil-1-(2-SO2CH3-4-2, 3-Cl2-fenil) propan-1, 3-dion, triketoni opisani u europskim patentnim prijavama 0 625 505 i 0 625 508, i posebno sulkotrion.
Konačno, predmet izuma je metoda zaštite biljaka od korova, naročito žitarica, pomoću herbicida ovog tipa, naznačena time da se herbicid aplicira na biljke transformirane u skladu s izumom, na oba načina, prije sijanja, prije izbijanja i nakon izbijanja žitarice. Predmet izuma je također upotreba HPPD gena kao marker gena tijekom ciklusa wtransformacije-regeneracije" biljne vrste i selekcije prema gornjem herbicidu.
Različiti aspekti izuma bit razumljiviji uz pomoć dolnjih eksperimentalnih primjera.
Primjer 1
Izolacija HPPD gena P. fluorescens A32
Počevši od amino-kiselinske sekvence od HPPD iz Pseudomonas sp. P.J. 874 (objavio Ruetchi U. et al.,: 1992, Eur. J. Biochem. 205, 459-466), uzeta je sekvenca od različitih oligonukleotida sa ciljem da se dio kodne sekvence HPPD P. fluorescens A 32 (koju je izolirao McKellar, R.C. 1982, J. Appl. Bacteriol., 53: 305-316) poveća pomoću PCR. Povećani fragment gena od te HPPD bio je upotrijebljen za zaklanjanje genomne banke P. fluorescens A32 i zatim za izolaciju gena koji kodira taj enzim.
A) Priprava genomne DNA P. fluorescens A32
Bakterije su uzgajane 48 sati pri 28°C u 40 ml M63 minimalnog supstrata (KH2PO4, 13,6 g/1, (NH4)2SO4 2 g/l, MgSO4 0,2 g/l, FeSO4 0,005 g/l, pH 7, plus L-tirozin 10 mM kao jedini izvora ugljika).
Nakon ispiranja stanice su skupljene u 1 ml pufera za ližu (100 mM tris HC1, pH 8,3, 1, 4 M NaCl i 10 mM EDTA) i inkubirane 10 minuta pri 65°C. Nakon obrade s fenol/kloroformom (24:1) i obrade s kloroformom, nukleinske kiseline su istaložene dodatkom jednog volumena izopropanola, zatim su uzete u 300 μm sterilne vode i konačno obrađene s 10 μg/ml RNAze. DNA je opet obrađena s fenol/kloroformom, kloroformom i ponovno istaložena dodatkom 1/10 volumena 3 M natrijevog acetata, pH 5 i 2 volumena etanola. DNA je zatim uzeta u sterilnu vodu i utvrđena.
B) Izbor oligonukleotida i sinteza
Počevši od amino-kisleinske sekvence HPPD od Pseudomonas sp. J.p. 874, odabrano je pet oligonukleotida, dva usmjerena u terminalnom NH2 smjeru proteina prema COOH završetku proteina i tri usmjerena u suprotnom smjeru (vidi sliku 1) . Izbor je bio diktiran s dva slijedeća pravila:
- stabilan 3 završetak oligonukleotida, to jest s najmanje dvije baze bez dvosmislenosti,
- najmanja moguća degeneracija.
Odabrani oligonukleotidi imaju slijedeće sekvence:
P1: 5' TA (C/T) GA (G/A) AA (C/T) CCIATGGG3'
P2 ; 5 GA (G/A) ACIGGICCIATGGA3'
P3 : 5' AA (C/T) TGCATIA (G/A) (G/A) AA (C/T) TC (C/T) TC3'
P4 : 5' AAIGCIAC (G/A) TG (C/T) TG (T/G/A) ATICC3'
P5 : 5' GC (C/T) TT (A/G) AA (A/G) TTICC (C/T) TCIC3'
Oni su sintetizirani na uređaju za sintezu DNA Cvclone plus tvrtke MTLLIPORE.
S tih pet oligonukleotida, povećani frakmenti koji se moraju dobiti terijski pomoću PCR započevši od sekvence SEQ ID br. l imaju slijedeće veličine:
s prajmerima P1 i P3 ----> približno 690 bp
s prajmerima P1 i P4 ----> približno 720 bp
s prajmerima P1 i P5 ----> približno 1000 bp
s prajmerima P1 i P3 ----> približno 390 bp
s prajmerima P2 i P4 ---->približno 420 bp
s prajmerima P1 i P5 ----> približno 700 bp
C) Povećavanje kodnog dijela HPPD P. fluorescens A32
Elongacija je bila provedena na uređaju PERKIN ELMER 9600 PCR i s PERKIN ELMER Tag polvmerazom s puferom pod standardnim uvjetima, to jest 50 μl reakcijske smjese od 200 uM dNTP, 20 μM prajmera, 2,5 jedinice Taq polimeraze i 2,5 μg DNA od P. fluorescens A32.
Korišten je program za elongaciju od 5 minuta pri 95°C i zatim 35<45 sek 95°C, 45 sek 49°C, 1 min 72°C> ciklusi praćeni su sa 5 minuta pri 72°C.
Pod tim uvjetima svi dobiveni elongirani fragmenti imaju veličinu usporedivi s gore datim teorijskim veličinama, što je dobar pokazatelj specifičnosti elongacija.
Dobiveni elongirani fragmenti sa skupinama fragmenata P1/P4, P1/P5 i P2/P4 su povezani u pBSII SK(-) nakon probave tog plazmida s Eco RV i obrade s terminalnom transferazom u prisutnosti ddTTP, kao što su opisali HOLTON T.A. i GRAHAM M.W. 1991, N.A.R., Vol. 19, br. 5, str, 1156.
Klon svakog od tri tipa je djelomično sekvenciran; to omogućuje da se potvrdi da je dio kodnog područja HPPD P. fluorescens A32 je bio dobro elongiran u tri slučaja. Fragment P1/P4 je zadržan kao uzorak da se zakloni parcijalnu genomnu banku P. fluorescens A32 i da se izolira kompletan HPPD gen.
D) Izolacija gena
Pomoću Southern pokazano je da fragment 7 Kbp, nakon probave DNA P. fluoroscencs A32 s restrikcijskim enzimom, BamHI hibridizira s uzorkom HPPB P1/P4. 400 ug DNA od P.
fluorescens A32 je tako probavljeno s restrikcijskim enzimom BamHI i DNA fragmenti, koji su činili do približno 7 Kbp, su očišćeni na agarnom gelu.
Ti fragmenti su povezani u pBSII SK(-), posljednji je probavljen s BamHI i defosforiliran obradom s alkalnom fosfatazom. Nakon transformacije u E. coli DH1Ob, parcijalna genomna banka je zaklonjena s uzorkom HPPD P1/P4.
Pozitivan klon je izoliran i nazvan pRP A. Njegova pojednostavljena karta prikazana na slici 2. Na toj karti pokazan je položaj kodnog dijela HPPD gena. On je sastavljen od 1077 nukleotida koji kodiraju 358 amino kiselina (vidi SEQ 1D br. 1). HPPD od P. fluorescens A32 ima dobru homologiju amino kiselina s HPPD od Pseudonomas sp. roda P.J. 874, u stvari postoji 92% podudarnosti između ta dva proteina (vidi sliku 3).
Primjer 2
Konstrukcija dvaju kimernih gena
Da bi se biljci podalo otpornost prema herbicidima koji inhibiraju HPPD, konstruirana su dva kimerna gena.
Prvi se sastoji od stavljanja kodnog dijela HPPD gena od P. fluorescens A32 pod kontrolu dvostrukog promotora histona (europska patentna prijava br. 0 507 698) i zatim pomoću translacijskog stimulatora tobacco etch virusa (TEV) (pRTL-GUS) Carrington i Freed, 1990; J. Virol. 64: 1590-1597)) s terminatorom gena nopalin sintaze. HPPD će se tada smjestiti u citoplazmu.
Drugi je identičan prvom, osim što je optimirani tranzitni peptida (OTP) umetnut između TEV aktivatora transkripcije i kodnog dijela HPPD (europska patentna prijava EP br. 0 508 909). HPPD će se tada smjestiti u kloroplast.
A) Kontsrukcija vektora pRPA-RD-153:
- pRDA-RD-11: Derivat od pBS-II SK(-) (katalog Stratagene 1212206) koji sadrži poliadenilacijsku stranu nopalin sintaze (NOS polyA) (europska patentna prijava EP br. 0 652 286) kloniran je između KpnI i Sali strana. Kpml strana je transformirana u Noti stranu obradom s T4 DNA polimerazom I u prisutnosti 150 μM deoksinukleotidnog trifosfata i zatim povezivanje s Noti linkerom (katalog Stratagene #1029). Tako je dobivena klonirana kazeta NOS polyA.
- pRPA-RD-127: Derivat od pRPA-BL-466 (europska patentna prijava EP br, 0 337 899) je kloniran u pRPA-RD-11 koji tvori kazetu ekspresije oksi gena i koji sadrži promotor male podjedinice ribuloza biskarboksilaze:
"promotor (SSU) - oksi gen - NOS polyA"
Za tvorbu tog plazmida, pRPA-BL-488 je bio probavljen sa Xbal i Hindlll za izolaciju fragmenta 1,9 kbp koji sadrži SSU promotor i oksigen, koji je bio povezan u plazmid pRPA-RD-11, probavljen je s kompatibilnim enzimima.
- pRPA-RD-11: To je derivat od pRPA-BL-488 (europska patentna prijava EP br. O 507 698) kloniran je u pRPA-RD-127 s tvorbom kazete ekspresije oksi gena s dvostrukim histonskim promotorm:
"double histone promotor- oxigene - NOS polyA"
Za proizvodnju tog plazmida, pRPA-BL-466 je probavljen s Hindlll, obrađen s Klenow-om i zatim ponovno probavljen s Ncol. Očišćen fragment od 1,35 kbp, koji sadrži histonski dvostruki promotor H3A748, povezan je s plazmidom pRPA-RD-127 koji je bio probavijen sa Xbal, obrađen s Klenow-om i ponovno probavijen s Ncol.
- pRPA-RD-153: To je derivat od pRPA-RD-132 Jcoji sadrži aktivator translacije tobacco etch virusa (TEV). pRTL-GUS (Carrington i Freed, 1990; J. Virol. 64: 1590-1597) je probavljen s Ncol i EcoRI i fragment 150 bp je povezan u pRPA-RD-132 probaljen s istim enzimima. Tako je stvorena kazeta ekspresije koja sadrži promotor:
"oble histone promotor - TEV - oxygene - NOS poly A"
B) Konstrukcija vektora pRPA-RD-185:
pUC19/GECA; Derivat od pUC-19 (katalog Gibco #15364-011) koji sadrži brojne klonirane strane. pUC-19 je probavljen s EcoRI i povezan s oligonukleotidnim linkerom 1.
Linker 1: AATTGGGCCA GTCAGGCCGT TTAAACCCTA GGGGGCCCG
CCCGGT CAGTCCGGCA AATTTGGGAT CCCCCGGGC TTAA
Selektirani klon sadrži stranu EcoRI i zatim polilinker koji sadrži slijedeće stranice: EcoRI, Apal, AvrII, PmeI, Sfil, Sacl, KpnI, Smal, BamHI, Xbal, SaII, Pst.I, SphI i HindIII.
pRPA-RD-185: To je derivat od pUC19/GECA koji sadrži modificirani polilinker. pUC19/GECA je probavljen s Hindlll i povezan s oligonukleotidnim linkerom 2:
Linker 2: AGCTTTTAAT TAAGGCGCGC CCTCGACCT GGTCAGGG
AAATTA ATTCCGCGCG GGAGCTCGGA CCAAGTCCC TCGA
Selektirani klon sadrži HindIII stranicu u središtu polilinkera koji sada sadrži slijedeće stranice: EcoRI, Apal, AvrII, PmeI, Sfil, SacI, KpnI, Smal, BamHI, Xbal, Sali, PstI, Sphl, HindIII, PacI, AscI, Xhol i EcoNi.
C) Konstrukcija vektora pRP T:
- pRP O: Derivat od pRPA-RD-153 koji sadrži kazetu ekspresije HPPD, dvostruki histonski promotor - TEV HPPD ggen - terminator Nos. Za proizvodnju pRP O, pRPA-RD153 je probavljen s Hindlll, obrađen s Klenow-om i zatim ponovno probavl-]en s Ncol da se odstrani oksi gen i nadomjesti s HPPD genom dobivenim od pRP A plazmida pomoću BstEIII probave, Klenow obrade i ponovne probave s Ncol.
- pRP R: Za dobivanje plazmida, pRP O je bio probavljen s PvuII i SacI, kimerni gen je očišćen i zatim povezan u pRPA-RD-185 i posljednji je probavljen s PvuIII i SacI.
- pRP T: Dobiven je povezivanjem kimernog gena koji je dobiven od pRP R nakon probave sa SacI i HindIII u plazmid pRPA-BL 150 α2 probavljen s istim enzimima (europska patentna prijava EP br. 0 508 909).
Kimerni gen od pRP T vektora imao je tako slijedeću strukturu:
[image]
D) Konstrukcija pRP V vektora
- pRP P: To je derivat od pRP-DR-7 (europska patentna prijava br. 0 652 286) koji sadrži optimirani tranzitni peptid iza kojeg slijedi HPPD gen. On je dobiven povezivanjem kodnog dijela HPPD dobivenog od pRP A probavom s BstEII i Ncol, Klenow obradom i od plazmida pRPA-RD-7, posljednji je probavljen sa SphI i AccI i obrađen s DNA-a polimerazom T4.
- pRP Q: Derivat od pRPA-RD-153 koji sadrži kazetu ekspresije HPPD, dvostruki histonski promotor - TEV -OTP -HPPD gen - Nos terminator. Za njegovu konstrukciju plazmid Prpa-RD-153 je probavljen sa Sali, obrađen s Klenow-om i zatim ponovno probavljen s Ncol da se odstrani oksigen i zamijeni s HPPD genom oslobođenim od pRP P plazmida probavom s BstEII, Klenow obradom i ponovno probavijen s Ncol.
- pRP S: Za njegovo dobivanje plazmid pRP Q probavljen je s PvuJI i SacI da se oslobodi kimerni gen, koji je povezan u pRPA-RD-185, posljednji je probavljen s PvuII i SacI.
- pRP V: Dobiven je povezivanjem kimernog gena oslobođenog od pRP S, nakon probave sa SacI i HindIII, u plazmid pRPA-BL 150 oc2 (europska patentna prijava EP br. 0 508 909).
Kimerni gen od pRP Q vektora imao je tako slijedeću strukturu:
[image]
Primjer 3
Transformacija industrijskog duhanskog PBD6
Da se odredi učinkovitost tih dvaju kimernih gena, oni su tranferirani u industrijski duhanski PBD6 u skladu s postupcima transformacije i regeneracije već opisanim u europskoj patentnoj prijavi EP br. 0 508 909.
1) Transformacija:
Vektor je uveden u ne-onkogeni rod Agrobacterium EHA 101 (Hood et al., 1987) koji nosi kozmid pTVK 291 (Komari et al., 1986). Postupak tranformacije temelji se na postupku koji su opisali Horsch R. et al., (1985), Science, 227 1229-1231.
2) Regeneracija:
Regeneracija duhanskog PBD6 (podrijetlo SEITA, Francuska) od lisnih eksplantata na osnovnom supstratu Murashige-a i Skooga (MS) koji sadrži 30 g/1 saharoze kao i 200 μg/ml kanamicina. Lisni eksplantati su selektirani na biljkama u stakleniku ili in vitro i transformirani u skladu s postupkom lisnih diskova (Science 1985, Vol. 227, str. 1229-1231) u tri uzastopna koraka: prvi uključuje indukciju zametaka tijekom 15 dana na MS supstratu kojem ie dodano 30 g/1 saharoze koja sadrži 0,05 mg/1 naftiloctene kiseline (NAA) i 2 mg/ benzilaminopurina (BAP). Zameci stvoreni tijekom te faze razvijaju se zatim uzgojem tijekom 10 dana na MS supstratu kojem je dodano 30 g/1 saharoze, ali koji nije sadržavao ni jedan hormon. Razvijeni zameci su zatim selektirani u uzgajani na MS supstratu za korijen na pola sadržaja soli, vitamina i šećera i koji također nije sadržavao nijedan hormon. Po završetku od približno 15 dana zameci s korijenom su zasađeni u zemlju.
Primjer 4
Mjerenje otpornosti duhana prema 4-[4-CF3-2-(metil-sulfonil)benzoilj-5-ciklopropilizoksazolu: obrada nakon izbijanja
Na živoj in-vitro kulturi, transformirane biljčice duhana aklimatizirane su u stakleniku (60% relativne vlage; temperatura: 20°C tijekom noći i 23°C tijekom dana) tijekom 5 tjedana i zatim su obrađene sa 4-[4-CF3-2-(metilsulfonil) benzoil]-5-ciklopropilizoksazolom.
Kontrolni duhan, ne-transformiran i obrađen sa 4-[4-CF3-2-(metilsulfonil)benzoilj-5-ciklopropilizoksazolom dozom u rasponu od 50 do 400 g/ha, razvija zelenilo za približno 72 sata, čiji intenzitet se za tjedan dana razvije u vrlo istaknutu uvelost (prekrije približno 80% graničnih listova).
Nakon transformacije, taj isti duhan, koji nad-umnožava HPPD od P. fluorescens, je vrlo dobro zaštićen od obrade sa 4-[4-CF3-2-(metilsulfonil)benzoil]-5-ciklo-propilizoksazolom u dozi od 400 g/ha.
Ako je nad-umnožen enzim u citoplazmi, to jest ako je transformacija provedena s genom kojeg nosi vektor pRP T, biljka tada pokazuje vrlo slabo zelenilo, pri čemu se sve zelenilo nalazi na sredini listova.
Ako je nad-umnožen enzim u kloroplastu, to jest ako je transformacija provedena s genom kojeg nosi vektor pRP V, biljka je odlično zaštićena i ne pokazuje nikakvih simptoma.
Primjer 5
Mjerenje otpornosti duhana prema 4-[4-CF3-2-(metil-sulfonil) benzoil]-5-ciklopropilizoksazolu: obrada prije izbijanja
a) Ispitivanje in vitro:
Sjeme duhana ubrano od biljaka iz ciklusa Mtransformirane generacije" i otpornih na obradu lišća s izoksaflutolom, upotrijebljeno je s dozom od 400 g/h opisanom u primjerima l do 3.
To sjeme bilo je posijano u kutije koje su sadržavale biljni agar od 10 g/l i izoksaflutol u različitim koncentracijama u rasponu od 0 do 1 mg/l. Klijanje je uslijedilo pri 25°C s fotoperiodom od 12 sata svjetla/12 sata mraka.
Prema tom protokolu, sjemenke divljeg duhama su proklijale kao i sjemenke dvaju tipova transgenskog duhana, to jest CY duhana, s lokalizacijom HPPD u citoplazmi, i CO duhan s lokalizacijom HPPD u kloroplastu.
[image]
°Simptomi koje pokazuju biljčice u smjeru klijanja su više ili manje signifikantne deformacije kotiledona i prije svega blijedilo tkiva koje je normalno fotosintetičko i stoga zeleno.
*75% sjemenki proklija, jer sjemenke od samo-oplodnje biljaka s jednog mjesta potječu iz ciklusa “transformacija-regeneracija” i stoga su bile pokazane samo one koje nose gen otpornosti na kromozomu. Radeći na isti način sa slijedećim proizvodima, proizvod br. 51 iz US patentna 4 780 127, dobiven je isti rezultat pri koncentraciji od 0 mg/l i 0,1 mg/l na divljem duhanu i CO duhanu.
b) Ispitivanje u stakleniku
Mjerenje je provedeno kao u primjeru 4, neovisno o obradi koja je bila provedena prije izbijanja, 24 sata prije sadnje. Divlje sijanje bilo je normalno provođene. Pod tim uvjetima, opaženo je da neobrađene, kontrolne sjemenke ne klijaju kod nijedne doze herbicida koja je jednaka najmanje 10 g/ha. Suprotno tome, CY duhani ne pokazuju nikakve simptome, kako su definirani u poglavlju a), sve do i uključiv 100 g/ha. Slično, CO duhani ne pokazuju nikakve simptome, kako su definirani u poglavlju a), sve do i uključiv 200 g/ha.
Ovi rezultati jasno pokazuju da HPPD gen od P. fluorescens podaje otpornost duhanu prema obradi s izoksaflutolom prije izbijanja. Ta otpornost je bolja ako je protein smješten u kloroplastu umjesto u citoplazmi.
Primjer 6
Sa ciljem proučavanja može li se HPPD gen Pseudomonas fluorescens upotrijebiti kao marker gen u pravcu ciklusa AV transformacija - regeneraci ja" biljnih vrsta, duhan je bio transformiran s HPPD genom i transformirane biljke su dobivene nakon selekcije prema izoksaflutolu.
Materijal, metode i rezultati
Kimerni gen pRP V, opisan u nastavku, transferiran je u industrijski duhan PBD6 sukladno postupku transformacije i regeneracije koji je već opisan u europskoj patentnoj prijavi EP br. 0 508 909.
Kimerni gen vektora pRP V ima slijedeću strukturu:
[image]
1) Transformacija:
Vektor je uveden u Agrobacterium EHA 101 ne-onkogenog podrijetla (Hoot et al., 1987) koji nosi kozmid pTVK 291 (Komari et al, 1986). Postupak transformacije temeljio se je na postupku kojeg su opisali Horsh et al., (1985).
2) Regeneracija:
Regeneracija duhana PBD6 (podrijetlo SEITA, Francuska) od lisnih eksplantata provedena je na Murashige-ovom i Skoogovorn (MS) osnovnom supstratu koji sadrži 30 g/l saharoze kao i 350 mg/l kefotaksima i 1 mg/l izoksaflutola. Lisni eksplantati su selektirani na biljakama u stakleniku ili in vitro i transformirani sukladno postupku folijarnih diskova (Science 1985, Vol. 227, str. 1229-1231) u tri uzastopne faze: prva uključuje indukciju zametka tijekom 15 dana u MS supstrat kojem je dodano 30 g/l saharoze koja sadrži 0,05 mg/l naftiloctene kiseline (NAA) i 2 mg/l benzilaminopurina (BAP) i 1 mg/l izoksaflutola. Zeleni zameci nastali tijekom te faze razvijeni su zatim uzgojem 10 dana na MS supstratu kojem je dodano 30 g/l saharoze i 1 mg/l izoksaflutola, ali nije sadržavao hormon. Razvijeni zameci su selektirani i uzgajani na MS supstratu za korijen s pola sadržaja soli, vitamina i šećera i 1 mg/l izoksaf lutola i koji nije sadržavao nijedan hormon. Otprilike pred kraj 15 dana, zameci korijena su stavljeni u zemlju.
Sve biljčice dobivene u skladu s ovim protokolom analizirane su pomoću PCR sa specifičnim prajmerima od HPPD od P. fluorescens. Tom PCR analizom može se potvrditi da su sve tako dobivene biljčice dobro integrirale HPPD gen.
U zaključku, pokus potvrđuje da se HPPD gen može upotrijebiti kao merker gen i da zajedno s tim genom izoksafiutoi može biti dobro sredstvo za selekciju.
Primjeri 7 i 8
Izolacija HPPD gena od Arabidopsis thaliana i HPPD gena mrkve (Dauous carotta)
a) Konstrukcija cDNA banaka
mRNAs ekstrahirana iz mladih biljčica Arabidopsis thaliana i mRNAs ekstrahirana iz stanica mrkve u kulturu koja služi za konstrukciju dviju cDNA banaka u vektoru Uni Zap™ XR, kojeg prodaje tvrtka Stratagen, zatim protokol koji preporuča ta tvrtka,
b) Zaklanjanje cDNA banaka
Te dvije banke bile su zaklonjene pomoću uzorka koji odgovara cDNA Arabidopsis thaliana parcijalne duljine, dobivene preko Arabidopsis Biological Resource Center (Oblo, USA) i indeksiran: EXT klon br. 91B13T7. Taj klon oblikovan je od približno 500 parova baza od kojih je samo 228 bilo sekvencirano u MSU-DOE Plant Research Laboratorv u kontekstu nasumičnog sekvenciranja cDNA Arabidopsis thaliana. Mi smo potpuno sekvencirali 500 parova baza prije upotrebe tog klona za zaklanjanje cDNA banaka Arabidopsis thaliana i mrkve klasičnim postupkom hibridizacije pločica liže (referenca ?).
c) Dobivena je cDNA Arabidopsis thaliana (SEQ ID br. 2) koja odgovara 1338 parova baza. Ta cDNA ima startni kodon inicijacije translacije u položaju 25 i završni kodon tranlacije u položaju 1336. Ta cDNA je stoga potpuna i kodira protein od 445 amino kiselina.
d) Dobivena je cDNA mrkve (Daucus carotta} SEQ ID br. 3) koja odgovara 1329 parova baze. Ta cDNA ima startni kodon inicijacije translacije u položaju l i završni kodon u položaju 1329. Ta cDNA je stoga potpuna i kodira za protein od 442 amino kiselina.
Prikazane su slijedeće sekvence:
SEQ ID br. 1: sekvenca HPPD gena Pseudomonas fluorescens A32
SEQ ID br. 2: sekvenca HPPD od Arabidopsis thalinana,
SEQ ID br. 3: cDNA sekvenca HPPD od Daucus carotta.
Slika 1 prikazuje proteinsku sekvencu HPPD od Pseudomonas sp. podrijetla P.J. 874 i teorijsku nukleotidnu sekvencu koja odgovara kodnom dijelu; pet oligonukleotida odabranih za provedbu elongacije dijela tog kodnog područja označeno je s pet strelica.
Slika 2 prikazuje kartiranje plazmida s genomnim DNA fragmentom od 7 kb koji uključuje HPPD gen od P. fluorescens A32.
Slika 3 daje usporedbu amino-kiselinske sekvence HPPD od P, fluorescens A32 i HPPD od Pseudomonas sp. podrijetla P.J. 874 (pokazane su samo divergentne amino kiseline, između dvije sekvence) kao i podudarne sekvence.
Prilog: Ispis sekvenci.
[image]
[image]
[image]
[image]

Claims (28)

1. Sekvenca izoliranog gena ne-humanog podrijetla ili iz ne-morske bakterije ili sekvenca koja se može hibridizirati s tom sekvencom, naznačena time, da umnožava hidroksifenilpiruvat dioksigenazu (HPPD).
2. Sekvenca prema zahtjevu 1, naznačena time, da je biljnog ili bakterijskog podrijetla.
3. Sekvenca prema zahtjevu 2, naznačena time, da potječe od Pseudomonas sp.
4. Sekvenca prema zahtjevu 3, naznačena time, da potječe od Pseudomonas fl uoroscens.
5. Sekvenca prema zahtjevu 1, naznačena time, da je biljnog podrijetla.
6. Sekvenca -prema zahtjevu 5, naznačena time, da potječe od Arabidopsis.
7. Sekvenca prema zahtjevu 5, naznačena time, da potječe od Umbellifera.
8. Metoda izolacije gena prema jednom od zahtjeva 1 do 4, naznačena time, da: - nekoliko oligonukleotida amino-kiselinske sekvence HPPD biraju se su kao prajmeri, - počevši od tih prajmera, povećani fragmenti sintetiziraju se su pomoću PCR, - gen se izolira tvorbom i zaklanjanjem genomne banke i - gen se klonira.
9. Kimerni gen za genetsku transformaciju biljaka koji uključuje, u smjeru transkripcije: - najmanje jednu sekvencu koja regulira promotor gena koji se sam prirodno umnaža u biljkama, - heterolognu kodnu sekvencu, - najmanje jednu poliadenilacijsku sekvencu, naznačen time, da kodna sekvenca je sekvenca gena koji umnaža hidroksifenilpiruvat dioksigenazu (HPPD).
10. Kimerni gen prema zahtjevu 9, naznačen time, da sekvenca koja regulira promotor potiče nadekspresiju kodne sekvence.
11. Kimerni gen prema zahtjevu 10, naznačen time, da sekvenca koja regulira promotor sadrži najmanje jedan promotor histona.
12. Kimerni gen prema jednom od zahtjeva 9 do 11, naznačen time, da između sekvence koja regulira promotor i kodne sekvence uključuje tranzitni pepetid.
13. Kimerni gen prema zahtjevu 9, naznačen time, da optimirani tranzitni peptid, koji se nalazi između sekvence koja regulira promotor i kodne sekvence, u smjeru transkripcije uključuje sekvencu koja kodira tranzitni peptid biljnog gena koji kodira za plastidni lokalizacijski enzim, dio sekvence N-terminalnog zrelog dijela biljnog gena koji kodira za plastidni lokalizacijski enzim, zatim sekvencu koja kodira za drugi tranzitni peptid biljnog gena koji kodira za plastidni lokalizacijski enzim.
14. Kimerni gen prema jednom od zahtjeva 9 do 13, naznačen time, da, između sekvence koja regulira promotor i kodne sekvence, uključuje sekvencu aktivatora transkripcije (stimulatora).
15. Vektor koji se može upotrijebiti za genetsku transformaciju biljaka, naznačen time, da sadrži kimerni gen u skladu s jednim od zahtjeva 9 do 14.
16. Biljna stanica, naznačena time, da sadrži kimerni gen u skladu s jednim od zahtjeva 9 do 14.
17. Biljka, naznačena time, da se regenerira iz stanica u skladu sa zahtjevom 16.
18. Biljka prema zahtjevu 17, naznačena time, da pripada porodici dvosupnica.
19. Metoda transformacije biljaka, da ih se učini otpornim prema HPPD inhibitorima, naznačena time, da se u biljnu stanicu uvodi gen ekspresije egzogene HPPD.
20. Metoda prema zahtjevu 19, naznačena time, da se transfer provodi s Agrobacterium tumefaciens ili Agrobacterium rhizogenes .
21. Metoda prema zahtjevu 19, naznačena time, da se transfer provodi dodatnim bombardiranjem uz pomoć nabijenih čestica DNA.
22. Metoda trnasformacije biljaka, naznačena time, da se gen ekspresije egzogene HPPD uvodi u biljnu stanicu kao selekcijski marker.
23. Metoda selektivne herbicidne obrade biljaka, naznačena time, da se na biljku aplicira inhibitor HPPD gena prema jednom od zahtjeva 17 i 19.
24. Metoda prema zahtjevu 23, naznačena -time, da je inhibitor HPPD gena izoksazol.
25. Metoda prema zahtjevu 24, naznačena time, da izoksazol je 4-[4-CF3-2-(metilsulfonil)benzoil]-5-ciklo-propil izoksazol.
26. Metoda prema zahtjevu 23, naznačena time, da je inhibitor HPPD gena diketonitril.
27. Metoda prema zahtjevu 23, naznačena time, da je inhibitor HPPD gena triketon.
28. Metoda prema zahtjevu 23, naznačena time, da je inhibitor HPPD gena sulkotrion.
HR9605944A 1995-06-02 1996-05-31 Dna sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are tolerant to certain herbicides HRP960245A2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9506800A FR2734840B1 (fr) 1995-06-02 1995-06-02 Gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant ce gene resistantes aux herbicides
FR9513570A FR2734841B1 (fr) 1995-06-02 1995-11-10 Gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant ce gene resistantes aux herbicides
FR9605944A FR2734842B1 (fr) 1995-06-02 1996-05-07 Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides

Publications (1)

Publication Number Publication Date
HRP960245A2 true HRP960245A2 (en) 1997-08-31

Family

ID=27253066

Family Applications (1)

Application Number Title Priority Date Filing Date
HR9605944A HRP960245A2 (en) 1995-06-02 1996-05-31 Dna sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are tolerant to certain herbicides

Country Status (28)

Country Link
US (1) US6268549B1 (hr)
EP (1) EP0828837B1 (hr)
JP (1) JPH11505729A (hr)
KR (1) KR100431366B1 (hr)
CN (1) CN1206358C (hr)
AR (1) AR002169A1 (hr)
AT (1) ATE383431T1 (hr)
AU (1) AU718982B2 (hr)
BG (1) BG102131A (hr)
BR (2) BRPI9608375C8 (hr)
CA (1) CA2219979C (hr)
CO (1) CO4520296A1 (hr)
CZ (1) CZ380997A3 (hr)
DE (1) DE69637402T2 (hr)
DK (1) DK0828837T3 (hr)
ES (1) ES2297840T3 (hr)
FR (1) FR2734842B1 (hr)
HR (1) HRP960245A2 (hr)
HU (1) HUP9900450A3 (hr)
MA (1) MA23884A1 (hr)
MX (1) MX9709310A (hr)
NZ (1) NZ311055A (hr)
PL (1) PL189955B1 (hr)
PT (1) PT828837E (hr)
SI (1) SI0828837T1 (hr)
SK (1) SK161597A3 (hr)
TR (1) TR199701492T1 (hr)
WO (1) WO1996038567A2 (hr)

Families Citing this family (462)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6087563A (en) * 1996-01-29 2000-07-11 Arizona Board Of Regents On Behalf Of The University Of Arizona Cloned arabidopsis p-hydroxyphenyl pyruvic acid dioxygenase DNA
JP2000513228A (ja) * 1996-06-27 2000-10-10 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー ｐ―ヒドロキシフェニルピルビン酸ジオキシゲナーゼの植物遺伝子
FR2751347B1 (fr) 1996-07-16 2001-12-07 Rhone Poulenc Agrochimie Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides
HUP0000053A3 (en) * 1996-07-25 2003-02-28 American Cyanamid Co Hppd gene and inhibitors
US20030041357A1 (en) * 1996-11-07 2003-02-27 Zeneca Limited Herbicide resistant plants
GEP20022841B (en) 1997-03-25 2002-11-25 Basf Ag Expression of Herbicide-Binding Polypeptides In Plants to Produce Herbicide Tolerance
DE19730066A1 (de) * 1997-07-14 1999-01-21 Basf Ag DNA-Sequenz codierend für eine Hydroxyphenylpyruvatdioxygenase und deren Überproduktion in Pflanzen
US7161064B2 (en) 1997-08-12 2007-01-09 North Carolina State University Method for producing stably transformed duckweed using microprojectile bombardment
FR2770854B1 (fr) * 1997-11-07 2001-11-30 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un tel gene, tolerantes aux herbicides
US6245968B1 (en) 1997-11-07 2001-06-12 Aventis Cropscience S.A. Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides
US6455762B1 (en) * 1997-11-12 2002-09-24 Board Of Control Of Michigan Technological University Methods of modifying lignin in plants by transformation with a 4-coumarate coenzyme a ligase nucleic acid
FR2771104B1 (fr) * 1997-11-17 2000-12-08 Rhone Poulenc Agrochimie Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs del'hppd
FR2778527A1 (fr) * 1998-05-18 1999-11-19 Rhone Poulenc Agrochimie Nouvelle methode de production de tocopherols dans les plantes et plantes obtenues
AU776316B2 (en) 1999-04-15 2004-09-02 Monsanto Company Nucleic acid sequences to proteins involved in tocopherol synthesis
FR2796954B1 (fr) * 1999-07-30 2003-10-31 Aventis Cropscience Sa Hydroxy-phenyl pyruvate dioxygenase fusionnee a un peptide signal, sequence d'adn et obtention de plantes contenant un tel gene, tolerantes aux herbicides
FR2803592A1 (fr) 2000-01-06 2001-07-13 Aventis Cropscience Sa Nouveaux derives de l'acide 3-hydroxypicolinique, leur procede de preparation et compositions fongicides les contenant.
CN1315523A (zh) * 2000-03-28 2001-10-03 上海博德基因开发有限公司 一种新的多肽——人过氧化酶18和编码这种多肽的多核苷酸
CN1315532A (zh) * 2000-03-29 2001-10-03 上海博德基因开发有限公司 一种新的多肽——人过氧化酶11和编码这种多肽的多核苷酸
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
US6768044B1 (en) * 2000-05-10 2004-07-27 Bayer Cropscience Sa Chimeric hydroxyl-phenyl pyruvate dioxygenase, DNA sequence and method for obtaining plants containing such a gene, with herbicide tolerance
AU9052201A (en) 2000-08-07 2002-02-18 Monsanto Technology Llc Methyl-d-erythritol phosphate pathway genes
FR2812883B1 (fr) 2000-08-11 2002-10-18 Aventis Cropscience Sa Utilisation d'inhibiteurs d'hppd comme agents de selection dans la transformation de plantes
EP1315801A1 (fr) * 2000-09-08 2003-06-04 Bayer CropScience S.A. Hydroxy-phenyl pyruvate dioxygenase fusionnee a un peptidesignal, sequence d 'adn et obtention de plantes contenant un tel gene,tolerantes aux herbicides
FR2815969B1 (fr) * 2000-10-30 2004-12-10 Aventis Cropscience Sa Plantes tolerantes aux herbicides par contournement de voie metabolique
FR2817557B1 (fr) * 2000-12-05 2005-05-06 Aventis Cropscience Sa Nouvelles cibles pour herbicides et plantes transgeniques resistantes a ces herbicides
AU2002249270B2 (en) 2001-02-22 2006-07-06 Biogemma S.A.S. Constitutive promoter from arabidopsis
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
CN1568141B (zh) 2001-05-09 2014-05-07 孟山都技术有限公司 Tyra基因及其应用
WO2003016482A2 (en) 2001-08-17 2003-02-27 Monsanto Technology Llc Methyltransferase genes and uses thereof
BR0213499A (pt) 2001-10-25 2004-11-03 Monsanto Technology Llc Metiltransferases aromáticas e seu uso
WO2003080647A2 (en) 2002-03-19 2003-10-02 Monsanto Technology, Llc Homogentisate prenyl transferase ('hpt') nucleic acids and polypeptides, and uses thereof
US7230165B2 (en) 2002-08-05 2007-06-12 Monsanto Technology Llc Tocopherol biosynthesis related genes and uses thereof
FR2844142B1 (fr) * 2002-09-11 2007-08-17 Bayer Cropscience Sa Plantes transformees a biosynthese de prenylquinones amelioree
US9453251B2 (en) * 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
FR2848064B1 (fr) * 2002-12-06 2006-01-13 Bayer Cropscience Sa Plantes legumineuses transplastomiques fertiles
FR2848570B1 (fr) 2002-12-12 2005-04-01 Bayer Cropscience Sa Cassette d'expression codant pour une 5-enol pyruvylshikimate-3-phosphate synthase (epsps) et plantes tolerantes aux herbicides la contenant
FR2848568B1 (fr) * 2002-12-17 2005-04-15 Rhobio Gene chimere permettant l'expression d'une hydroxy-phenyl pyruvate dioxygenase dans les plastes et plantes transplastomiques contenant un tel gene tolerantes aux herbicides
CN1863914B (zh) 2003-04-29 2011-03-09 先锋高级育种国际公司 新的草甘膦-n-乙酰转移酶(gat)基因
ES2275365B1 (es) * 2003-07-25 2008-04-16 Universidad De Cordoba Molecula de adn que codifica una p-hidroxifenilpiruvato dioxigenasa de chlamydomonas y sus aplicaciones.
ES2707786T3 (es) 2004-01-16 2019-04-05 Pfenex Inc Expresión de proteínas de mamífero en Pseudomonas fluorescens
CN101031655A (zh) 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
WO2006132270A1 (ja) * 2005-06-10 2006-12-14 Kyoto University 除草剤抵抗性遺伝子
AR056438A1 (es) * 2005-08-01 2007-10-10 Basf Ag Un metodo para controlar malezas
AR055593A1 (es) * 2005-08-01 2007-08-29 Basf Ag Un metodo para controlar malezas
AR056436A1 (es) * 2005-08-01 2007-10-10 Basf Ag Un metodo para controlar malezas
US8993846B2 (en) 2005-09-06 2015-03-31 Monsanto Technology Llc Vectors and methods for improved plant transformation efficiency
EP1929017B1 (en) 2005-09-16 2011-12-07 Bayer CropScience AG Transplastomic plants expressing lumen-targeted protein
AU2006302969B2 (en) 2005-10-13 2011-09-22 Monsanto Technology, Llc Methods for producing hybrid seed
ATE500337T1 (de) 2006-05-18 2011-03-15 Biogemma Fr Verfahren zur durchführung homologer rekombinationen in pflanzen
MX2008015742A (es) 2006-06-06 2008-12-19 Monsanto Technology Llc Metodo de seleccion de celulas transformadas.
US7855326B2 (en) * 2006-06-06 2010-12-21 Monsanto Technology Llc Methods for weed control using plants having dicamba-degrading enzymatic activity
US7951995B2 (en) 2006-06-28 2011-05-31 Pioneer Hi-Bred International, Inc. Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof
US7939721B2 (en) 2006-10-25 2011-05-10 Monsanto Technology Llc Cropping systems for managing weeds
CL2007003744A1 (es) 2006-12-22 2008-07-11 Bayer Cropscience Ag Composicion que comprende un derivado 2-piridilmetilbenzamida y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos.
CL2007003743A1 (es) * 2006-12-22 2008-07-11 Bayer Cropscience Ag Composicion que comprende fenamidona y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos.
US7838729B2 (en) * 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
EP2450448B1 (en) 2007-03-09 2014-09-17 Monsanto Technology LLC Methods for plant transformation using spectinomycin selection
EP1969934A1 (de) * 2007-03-12 2008-09-17 Bayer CropScience AG 4-Cycloalkyl-oder 4-arylsubstituierte Phenoxyphenylamidine und deren Verwendung als Fungizide
EP1969930A1 (de) 2007-03-12 2008-09-17 Bayer CropScience AG Phenoxyphenylamidine und deren Verwendung als Fungizide
EP2120558B1 (de) * 2007-03-12 2016-02-10 Bayer Intellectual Property GmbH 3,4-Disubstituierte Phenoxyphenylamidin-Derivate und deren Verwendung als Fungizide
WO2008110279A1 (de) 2007-03-12 2008-09-18 Bayer Cropscience Ag Dihalogenphenoxyphenylamidine und deren verwendung als fungizide
EP1969931A1 (de) * 2007-03-12 2008-09-17 Bayer CropScience Aktiengesellschaft Fluoalkylphenylamidine und deren Verwendung als Fungizide
EP1969929A1 (de) 2007-03-12 2008-09-17 Bayer CropScience AG Substituierte Phenylamidine und deren Verwendung als Fungizide
JP2010524869A (ja) 2007-04-19 2010-07-22 バイエル・クロツプサイエンス・アクチエンゲゼルシヤフト チアジアゾリルオキシフェニルアミジンおよび殺菌剤としてのこれらの使用
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
KR101530395B1 (ko) 2007-04-27 2015-06-19 피페넥스 인크. 향상된 수율 및(또는) 품질로 이종 단백질을 발현하는 특정 균주를 동정하기 위해 미생물 숙주를 신속히 스크리닝하는 방법
DE102007045922A1 (de) * 2007-09-26 2009-04-02 Bayer Cropscience Ag Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
DE102007045953B4 (de) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
DE102007045919B4 (de) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
DE102007045957A1 (de) 2007-09-26 2009-04-09 Bayer Cropscience Ag Wirkstoffkombinationen mit insektiziden und akarziden Eigenschaften
DE102007045956A1 (de) * 2007-09-26 2009-04-09 Bayer Cropscience Ag Wirkstoffkombination mit insektiziden und akariziden Eigenschaften
DE102007045920B4 (de) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Synergistische Wirkstoffkombinationen
DE102007045955A1 (de) 2007-09-26 2009-04-09 Bayer Cropscience Ag Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
EP2090168A1 (de) 2008-02-12 2009-08-19 Bayer CropScience AG Methode zur Verbesserung des Pflanzenwachstums
KR20100074229A (ko) * 2007-10-02 2010-07-01 바이엘 크롭사이언스 아게 식물 성장 촉진방법
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
EP2072506A1 (de) 2007-12-21 2009-06-24 Bayer CropScience AG Thiazolyloxyphenylamidine oder Thiadiazolyloxyphenylamidine und deren Verwendung als Fungizide
EP3095869B1 (en) * 2008-04-14 2019-06-19 BASF Agricultural Solutions Seed US LLC Mutated hydroxyphenylpyruvate dioxygenase, dna sequence and isolation of plants which are tolerant to hppd inhibitor herbicides
EP2113172A1 (de) * 2008-04-28 2009-11-04 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
MX2010014319A (es) 2008-07-08 2011-05-19 Geneuro Sa Uso terapeutico de ligando especifico en enfermedades asociadas con msrv.
US8697941B2 (en) 2008-07-23 2014-04-15 Pioneer Hi-Bred International, Inc. Molecular markers linked to PPO inhibitor tolerance in soybeans
US8748695B2 (en) 2008-07-23 2014-06-10 Pioneer Hi-Bred International, Inc. Molecular markers linked to PPO inhibitor tolerance in soybeans
EP2168434A1 (de) 2008-08-02 2010-03-31 Bayer CropScience AG Verwendung von Azolen zur Steigerung der Resistenz von Pflanzen oder Pflanzenteilen gegenüber abiotischem Stress
BRPI0917094B1 (pt) 2008-08-08 2018-01-16 Bayer Cropscience Nv Métodos para identificação de fibra de planta processada, para análise do genoma de uma planta de algodão produtora de fibra, e para o isolamento de DNA de ocorrência natural de fibras de planta de algodão processadas, uso dos referidos métodos, bem como métodos para o isolamento de um DNA de ocorrência natural de tecido ou de pano tricotado, para determinar as quantidades relativas de diferentes fibras de planta de algodão em uma mistura de fibras de algodão processadas, e para certificar a identidade de fibras de algodão comercializadas
US20110190365A1 (en) 2008-08-14 2011-08-04 Bayer Crop Science Ag Insecticidal 4-phenyl-1H-pyrazoles
DE102008041695A1 (de) * 2008-08-29 2010-03-04 Bayer Cropscience Ag Methoden zur Verbesserung des Pflanzenwachstums
CA2737939C (en) 2008-09-26 2021-04-27 Basf Agrochemical Products B.V. Herbicide-resistant ahas-mutants and methods of use
AR075466A1 (es) 2008-10-22 2011-04-06 Basf Se Uso de herbicidas tipo auxina en plantas cultivadas
AR075465A1 (es) 2008-10-22 2011-04-06 Basf Se Uso de herbicidas de sulfonilurea en plantas cultivadas
EP2201838A1 (de) 2008-12-05 2010-06-30 Bayer CropScience AG Wirkstoff-Nützlings-Kombinationen mit insektiziden und akariziden Eigenschaften
EP2198709A1 (de) 2008-12-19 2010-06-23 Bayer CropScience AG Verfahren zur Bekämpfung resistenter tierischer Schädlinge
EP2204094A1 (en) 2008-12-29 2010-07-07 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants Introduction
EP2223602A1 (de) 2009-02-23 2010-09-01 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials genetisch modifizierter Pflanzen
US9763451B2 (en) 2008-12-29 2017-09-19 Bayer Intellectual Property Gmbh Method for improved use of the production potential of genetically modified plants
EP2039770A2 (en) 2009-01-06 2009-03-25 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants
EP2039771A2 (en) 2009-01-06 2009-03-25 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants
EP2039772A2 (en) 2009-01-06 2009-03-25 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants introduction
AR074941A1 (es) 2009-01-07 2011-02-23 Bayer Cropscience Sa Plantas transplastomicas exentas de marcador de seleccion
US8487118B2 (en) 2009-01-19 2013-07-16 Bayer Cropscience Ag Cyclic diones and their use as insecticides, acaricides and/or fungicides
EP2227951A1 (de) 2009-01-23 2010-09-15 Bayer CropScience AG Verwendung von Enaminocarbonylverbindungen zur Bekämpfung von durch Insekten übertragenen Viren
US8349884B2 (en) 2009-01-28 2013-01-08 Bayer Cropscience Ag Fungicide N-cycloalkyl-N-bicyclimethylene-carboxamide derivatives
AR075126A1 (es) 2009-01-29 2011-03-09 Bayer Cropscience Ag Metodo para el mejor uso del potencial de produccion de plantas transgenicas
JP5728735B2 (ja) 2009-02-17 2015-06-03 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ 殺菌性ｎ−（フェニルシクロアルキル）カルボキサミド、ｎ−（ベンジルシクロアルキル）カルボキサミド及びチオカルボキサミド誘導体
EP2218717A1 (en) 2009-02-17 2010-08-18 Bayer CropScience AG Fungicidal N-((HET)Arylethyl)thiocarboxamide derivatives
TW201031331A (en) 2009-02-19 2010-09-01 Bayer Cropscience Ag Pesticide composition comprising a tetrazolyloxime derivative and a fungicide or an insecticide active substance
DE102009001469A1 (de) 2009-03-11 2009-09-24 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
DE102009001681A1 (de) 2009-03-20 2010-09-23 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
DE102009001732A1 (de) 2009-03-23 2010-09-30 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
DE102009001730A1 (de) 2009-03-23 2010-09-30 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
DE102009001728A1 (de) 2009-03-23 2010-09-30 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
CN102448304B (zh) 2009-03-25 2015-03-11 拜尔农作物科学股份公司 具有杀昆虫和杀螨特性的活性成分结合物
US9012360B2 (en) 2009-03-25 2015-04-21 Bayer Intellectual Property Gmbh Synergistic combinations of active ingredients
NZ595345A (en) 2009-03-25 2014-01-31 Bayer Cropscience Ag Active ingredient combinations with insecticidal and acaricidal properties
US8828907B2 (en) 2009-03-25 2014-09-09 Bayer Cropscience Ag Active ingredient combinations having insecticidal and acaricidal properties
WO2010108504A1 (de) 2009-03-25 2010-09-30 Bayer Cropscience Ag Wirkstoffkombinationen mit insektiziden und akariziden eigenschaften
EP2232995A1 (de) 2009-03-25 2010-09-29 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
EP2239331A1 (en) 2009-04-07 2010-10-13 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants
JP5771189B2 (ja) 2009-05-06 2015-08-26 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ シクロペンタンジオン化合物、ならびにこの殺虫剤、殺ダニ剤および／または抗真菌剤としての使用
EP2251331A1 (en) 2009-05-15 2010-11-17 Bayer CropScience AG Fungicide pyrazole carboxamides derivatives
AR076839A1 (es) 2009-05-15 2011-07-13 Bayer Cropscience Ag Derivados fungicidas de pirazol carboxamidas
EP2258859A1 (en) 2009-05-19 2010-12-08 Genoplante-Valor Method for performing homologous recombination
EP2255626A1 (de) 2009-05-27 2010-12-01 Bayer CropScience AG Verwendung von Succinat Dehydrogenase Inhibitoren zur Steigerung der Resistenz von Pflanzen oder Pflanzenteilen gegenüber abiotischem Stress
EP2437595B1 (de) * 2009-06-02 2018-10-31 Bayer CropScience AG Verwendung von fluopyram zur kontrolle von sclerotinia ssp
CN102458111B (zh) 2009-06-25 2014-07-30 巴斯夫欧洲公司 农化混合物在提高植物健康中的用途
CN104430378A (zh) 2009-07-16 2015-03-25 拜尔农作物科学股份公司 含苯基三唑的协同活性物质结合物
WO2011015524A2 (en) 2009-08-03 2011-02-10 Bayer Cropscience Ag Fungicide heterocycles derivatives
EP2292094A1 (en) 2009-09-02 2011-03-09 Bayer CropScience AG Active compound combinations
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
US8153132B2 (en) * 2009-10-30 2012-04-10 Ms Technologies, Inc. Antibodies immunoreactive with mutant hydroxypenylpyruvate dioxygenase
JP2013510113A (ja) 2009-11-06 2013-03-21 ビーエーエスエフ ソシエタス・ヨーロピア ４−ヒドロキシ安息香酸及び選択された殺有害生物剤の結晶複合体
TW201142029A (en) 2009-11-24 2011-12-01 Univ Leuven Kath Banana promoters
JP2013512935A (ja) 2009-12-08 2013-04-18 ビーエーエスエフ ソシエタス・ヨーロピア 殺害虫混合物
CN102647903B (zh) 2009-12-08 2015-09-16 巴斯夫欧洲公司 农药混合物
EP2343280A1 (en) 2009-12-10 2011-07-13 Bayer CropScience AG Fungicide quinoline derivatives
ES2658990T3 (es) * 2009-12-23 2018-03-13 Bayer Intellectual Property Gmbh Plantas tolerantes a herbicidas inhibidores de HPPD
UY33141A (es) 2009-12-23 2011-07-29 Bayer Cropscience Ag Plantas tolerantes a herbicidas inhibidores de las hppd
AR079882A1 (es) * 2009-12-23 2012-02-29 Bayer Cropscience Ag Plantas tolerantes a herbicidas inhibidores de las hppd
UY33140A (es) * 2009-12-23 2011-07-29 Bayer Cropscience Ag Plantas tolerantes a herbicidas inhibidores de las hppd
AR079883A1 (es) * 2009-12-23 2012-02-29 Bayer Cropscience Ag Plantas tolerantes a herbicidas inhibidores de las hppd
WO2011080256A1 (en) 2009-12-28 2011-07-07 Bayer Cropscience Ag Fungicide hydroximoyl-tetrazole derivatives
TWI483679B (zh) 2009-12-28 2015-05-11 Bayer Ip Gmbh 殺真菌劑肟醯基（ｈｙｄｒｏｘｉｍｏｙｌ）－雜環衍生物
CN102725282B (zh) 2009-12-28 2015-12-16 拜尔农科股份公司 杀真菌剂肟基-四唑衍生物
NZ601341A (en) 2010-01-22 2014-02-28 Bayer Ip Gmbh Acaricide and/or insecticide active substance combinations
WO2011089021A1 (en) 2010-01-25 2011-07-28 Bayer Bioscience N.V. Methods for manufacturing plant cell walls comprising chitin
WO2011094199A1 (en) * 2010-01-26 2011-08-04 Pioneer Hi-Bred International, Inc. Polynucleotide and polypeptide sequences associated with herbicide tolerance
US20120311743A1 (en) 2010-02-02 2012-12-06 Bayer Cropscience Ag Soybean transformation using hppd inhibitors as selection agents
ES2523503T3 (es) 2010-03-04 2014-11-26 Bayer Intellectual Property Gmbh 2-Amidobencimidazoles sustituidos con fluoroalquilo y su uso para el aumento de la tolerancia al estrés en plantas
ES2809679T3 (es) 2010-03-08 2021-03-05 Monsanto Technology Llc Moléculas polinucleotídicas para la regulación génica en plantas
AR080684A1 (es) 2010-03-18 2012-05-02 Bayer Cropscience Ag Aril- y hetarilsulfonamidas como sustancias activas contra un estres abiotico de plantas
JP2013523795A (ja) 2010-04-06 2013-06-17 バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー 植物のストレス耐性を増強させるための４−フェニル酪酸及び／又はその塩の使用
MX342482B (es) 2010-04-09 2016-09-30 Bayer Ip Gmbh Uso de derivados de acido (1-cianociclopropil)-fenilfosfinico, sus esteres y/o sus sales para potenciar la tolerancia de plantas al estres abiotico.
EP2563784A1 (en) 2010-04-28 2013-03-06 Bayer CropScience AG Fungicide hydroximoyl-heterocycles derivatives
WO2011134911A2 (en) 2010-04-28 2011-11-03 Bayer Cropscience Ag Fungicide hydroximoyl-tetrazole derivatives
JP2013525400A (ja) 2010-04-28 2013-06-20 バイエル・クロップサイエンス・アーゲー 殺菌剤ヒドロキシモイル−複素環誘導体
US20130053243A1 (en) * 2010-05-04 2013-02-28 Basf Se Plants having increased tolerance to herbicides
WO2011151261A2 (en) 2010-05-31 2011-12-08 Basf Se Method for increasing the health of a plant
ES2533026T3 (es) 2010-06-03 2015-04-07 Bayer Intellectual Property Gmbh N-[(het)arilalquil)]pirazol (tio)carboxamidas y sus análogos heterosustituidos
EP2576512A1 (en) 2010-06-03 2013-04-10 Bayer Intellectual Property GmbH O-cyclopropylcyclohexyl-carboxanilides and their use as fungicides
UA110703C2 (uk) 2010-06-03 2016-02-10 Байєр Кропсайнс Аг Фунгіцидні похідні n-[(тризаміщений силіл)метил]-карбоксаміду
WO2011151370A1 (en) 2010-06-03 2011-12-08 Bayer Cropscience Ag N-[(het)arylalkyl)] pyrazole (thio)carboxamides and their heterosubstituted analogues
CN103119169B (zh) 2010-06-09 2018-11-20 拜尔作物科学公司 植物基因组改造中常用的在核苷酸序列上修饰植物基因组的方法和工具
WO2011154158A1 (en) 2010-06-09 2011-12-15 Bayer Bioscience N.V. Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
WO2011161132A1 (en) 2010-06-25 2011-12-29 Basf Se Pesticidal mixtures
WO2011161131A1 (en) 2010-06-25 2011-12-29 Basf Se Herbicidal mixtures
EP2402446A1 (en) 2010-06-30 2012-01-04 Genoplante-Valor Gene involved in the development of the seed
JP2013531502A (ja) 2010-07-08 2013-08-08 バイエル・クロップサイエンス・エヌ・ヴェー グルコシノレート輸送タンパク質及びその使用
RU2013107369A (ru) 2010-07-20 2014-08-27 Байер Кропсайенс Аг Бензоциклоалкеныв качестве противогрибковых средств
AU2011289283B2 (en) 2010-08-13 2015-04-30 Pioneer Hi-Bred International, Inc. Chimeric promoters and methods of use
WO2012022729A2 (en) 2010-08-20 2012-02-23 Basf Se Method for improving the health of a plant
WO2012025472A1 (en) 2010-08-24 2012-03-01 Basf Se Agrochemical mixtures for increasing the health of a plant
CN103328484A (zh) 2010-09-03 2013-09-25 拜耳知识产权有限责任公司 二噻英四(硫代)甲酰亚胺
EP2611300B1 (de) 2010-09-03 2016-04-06 Bayer Intellectual Property GmbH Substituierte anellierte dihydropyrimidinonderivate
JP2012062267A (ja) 2010-09-15 2012-03-29 Bayer Cropscience Ag 殺虫性ピロリンｎ−オキサイド誘導体
JP2012082186A (ja) 2010-09-15 2012-04-26 Bayer Cropscience Ag 殺虫性アリールピロリジン類
EP2460406A1 (en) 2010-12-01 2012-06-06 Bayer CropScience AG Use of fluopyram for controlling nematodes in nematode resistant crops
US20140056866A1 (en) 2010-09-22 2014-02-27 Bayer Intellectual Property Gmbh Use of biological or chemical control agents for controlling insects and nematodes in resistant crops
US9408391B2 (en) 2010-10-07 2016-08-09 Bayer Intellectual Property Gmbh Fungicide composition comprising a tetrazolyloxime derivative and a thiazolylpiperidine derivative
WO2012045737A1 (en) 2010-10-07 2012-04-12 Basf Se Use of strobilurins for increasing the gluten strength in winter cereals
BR112013009580B1 (pt) 2010-10-21 2018-06-19 Bayer Intellectual Property Gmbh Composto de fómrula (i), composição fungicida e método para controlar fungos fitopatogênicos
AR083501A1 (es) 2010-10-21 2013-02-27 Bayer Cropscience Ag 1-(heterociclo carbonil)piperidinas
JP2013542215A (ja) 2010-11-02 2013-11-21 バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー Ｎ−ヘタリールメチルピラゾリルカルボキサミド類
EP2669371A1 (en) 2010-11-10 2013-12-04 Bayer CropScience AG HPPD variants and methods of use
WO2012062749A1 (de) 2010-11-12 2012-05-18 Bayer Cropscience Ag Benzimidazolidinone verwendbar als fungizide
CN103391925B (zh) 2010-11-15 2017-06-06 拜耳知识产权有限责任公司 5‑卤代吡唑甲酰胺
EP2640191A1 (en) 2010-11-15 2013-09-25 Bayer Intellectual Property GmbH 5-halogenopyrazole(thio)carboxamides
ES2643128T3 (es) 2010-11-15 2017-11-21 Bayer Intellectual Property Gmbh Cianoenaminas y su uso como fungicidas
BR112013012080A2 (pt) 2010-11-15 2016-07-19 Bayer Ip Gmbh n-aril pirazol (tio) carboxamidas
EP2640693A1 (en) 2010-11-15 2013-09-25 Bayer Intellectual Property GmbH Cyanoenamines and their use as fungicides
EP2454939A1 (en) 2010-11-18 2012-05-23 Bayer CropScience AG Post-harvest treatment
CA2810180C (en) 2010-11-24 2015-07-28 Pioneer Hi-Bred International, Inc. Brassica gat event dp-073496-4 and compositions and methods for the identification and/or detection thereof
CA2818918A1 (en) 2010-11-24 2012-05-31 Pioneer Hi-Bred International, Inc. Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof
CN103391926A (zh) 2010-11-30 2013-11-13 拜耳知识产权有限责任公司 嘧啶衍生物及其作为病虫害防治剂的应用
EP2460407A1 (de) 2010-12-01 2012-06-06 Bayer CropScience AG Wirkstoffkombinationen umfassend Pyridylethylbenzamide und weitere Wirkstoffe
AU2011334989A1 (en) 2010-12-01 2013-06-13 Bayer Intellectual Property Gmbh Use of fluopyram for controlling nematodes in crops and for increasing yield
WO2012074868A2 (en) 2010-12-03 2012-06-07 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
TW201231655A (en) * 2010-12-15 2012-08-01 Syngenta Participations Ag Soybean event SYHT04R and compositions and methods for detection thereof
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
MX2013006920A (es) 2010-12-22 2013-07-22 Basf Se Mezclas agroquimicas para aumentar la salud de una planta.
JP5842594B2 (ja) 2010-12-27 2016-01-13 住友化学株式会社 ピリダジノン化合物、それを含有する除草剤及び有害節足動物防除剤
RU2604793C2 (ru) * 2010-12-28 2016-12-10 ЭсДиЭс БАЙОТЕК К. К. Растение, обладающее повышенной устойчивостью или чувствительностью к ингибитору 4-hppd
US20130289077A1 (en) 2010-12-29 2013-10-31 Juergen Benting Fungicide hydroximoyl-tetrazole derivatives
EP2474542A1 (en) 2010-12-29 2012-07-11 Bayer CropScience AG Fungicide hydroximoyl-tetrazole derivatives
EP2471363A1 (de) 2010-12-30 2012-07-04 Bayer CropScience AG Verwendung von Aryl-, Heteroaryl- und Benzylsulfonamidocarbonsäuren, -carbonsäureestern, -carbonsäureamiden und -carbonitrilen oder deren Salze zur Steigerung der Stresstoleranz in Pflanzen
WO2012088645A1 (en) 2010-12-31 2012-07-05 Bayer Cropscience Ag Method for improving plant quality
EP2668278A1 (en) 2011-01-24 2013-12-04 Bayer CropScience NV Use of the rd29 promoter or fragments thereof for stress-inducible expression of transgenes in cotton
CN103415213A (zh) 2011-02-15 2013-11-27 拜耳知识产权有限责任公司 含有二硫杂环己二烯四甲酰亚胺杀真菌剂和除草剂、安全剂或植物生长调节剂的协同组合
EP2494867A1 (de) 2011-03-01 2012-09-05 Bayer CropScience AG Halogen-substituierte Verbindungen in Kombination mit Fungiziden
US20130345058A1 (en) 2011-03-10 2013-12-26 Wolfram Andersch Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds
US20140005230A1 (en) 2011-03-14 2014-01-02 Juergen Benting Fungicide hydroximoyl-tetrazole derivatives
EP2502495A1 (en) 2011-03-16 2012-09-26 Bayer CropScience AG Use of a dithiino-tetracarboxamide for the protection of harvested products against phytopathogenic fungi
US8648230B2 (en) 2011-03-18 2014-02-11 Ms Technologies, Llc Regulatory regions preferentially expressing in non-pollen plant tissue
PL2688406T3 (pl) 2011-03-25 2015-10-30 Bayer Ip Gmbh Zastosowanie N-(tetrazol-4-ilo)- lub N-(triazol-3-ilo)arylokarboksamidów lub ich soli do zwalczania niepożądanych roślin w obszarach transgenicznych roślin uprawnych tolerancyjnych na środki chwastobójcze będące inhibitorem HPPD
EA201391302A1 (ru) * 2011-03-25 2014-04-30 Байер Интеллектуэль Проперти Гмбх Применение n-(1,2,5-оксадиазол-3-ил)бензамидов для борьбы с нежелательными растениями в районах произрастания трансгенных культурных растений, устойчивых к гербицидам - ингибиторам hppd
MX350825B (es) 2011-03-31 2017-09-22 Bayer Ip Gmbh 3-fenilisoxazolin-5-carboxamidas y 3-fenilisoxazolin-5-tioamidas de accion herbicida y fungicida.
CN103517900A (zh) 2011-04-08 2014-01-15 拜耳知识产权有限责任公司 杀真菌剂肟基-四唑衍生物
EP2511255A1 (de) 2011-04-15 2012-10-17 Bayer CropScience AG Substituierte Prop-2-in-1-ol- und Prop-2-en-1-ol-Derivate
AR085568A1 (es) 2011-04-15 2013-10-09 Bayer Cropscience Ag 5-(biciclo[4.1.0]hept-3-en-2-il)-penta-2,4-dienos y 5-(biciclo[4.1.0]hept-3-en-2-il)-pent-2-en-4-inos sustituidos como principios activos contra el estres abiotico de las plantas
AR085585A1 (es) 2011-04-15 2013-10-09 Bayer Cropscience Ag Vinil- y alquinilciclohexanoles sustituidos como principios activos contra estres abiotico de plantas
AR090010A1 (es) 2011-04-15 2014-10-15 Bayer Cropscience Ag 5-(ciclohex-2-en-1-il)-penta-2,4-dienos y 5-(ciclohex-2-en-1-il)-pent-2-en-4-inos sustituidos como principios activos contra el estres abiotico de las plantas, usos y metodos de tratamiento
CN103635089B (zh) 2011-04-22 2016-12-14 拜耳知识产权有限责任公司 包含(硫代)羧酰胺衍生物和杀真菌化合物的活性化合物复配物
WO2012168124A1 (en) 2011-06-06 2012-12-13 Bayer Cropscience Nv Methods and means to modify a plant genome at a preselected site
CN103957711A (zh) 2011-07-04 2014-07-30 拜耳知识产权有限责任公司 取代的异喹啉酮、异喹啉二酮、异喹啉三酮和二氢异喹啉酮或其各自的盐作为活性剂对抗植物非生物胁迫的用途
EP2736333A1 (en) 2011-07-27 2014-06-04 Bayer Intellectual Property GmbH Seed dressing for controlling phytopathogenic fungi
WO2013020985A1 (en) 2011-08-10 2013-02-14 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
US20140215655A1 (en) 2011-08-12 2014-07-31 Bayer Cropscience Nv Guard cell-specific expression of transgenes in cotton
EP2748161A1 (en) 2011-08-22 2014-07-02 Bayer Intellectual Property GmbH Fungicide hydroximoyl-tetrazole derivatives
CN103981149A (zh) 2011-08-22 2014-08-13 拜尔作物科学公司 修饰植物基因组的方法和手段
EP2561759A1 (en) 2011-08-26 2013-02-27 Bayer Cropscience AG Fluoroalkyl-substituted 2-amidobenzimidazoles and their effect on plant growth
WO2013034621A1 (en) 2011-09-09 2013-03-14 Bayer Intellectual Property Gmbh Acyl-homoserine lactone derivatives for improving plant yield
CN103874681B (zh) 2011-09-12 2017-01-18 拜耳知识产权有限责任公司 杀真菌性4‑取代的‑3‑{苯基[(杂环基甲氧基)亚氨基]甲基}‑1,2,4‑噁二唑‑5(4h)‑酮衍生物
EP3296402B1 (en) 2011-09-13 2020-04-15 Monsanto Technology LLC Methods and compositions for weed control
BR112014005979A8 (pt) 2011-09-13 2017-09-12 Monsanto Technology Llc Métodos e composições quimicas agricolas para controle de planta, método de redução de expressão de um gene ppg oxidase em uma planta, cassete de expressão microbiana, método para fazer um polinucleotídeo, método de identificação de polinucleotídeos úteis na modulação de expressão do gene ppg oxidase e mistura herbicida
UA116091C2 (uk) 2011-09-13 2018-02-12 Монсанто Текнолоджи Ллс Способи та композиції для боротьби з бур'янами
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
EP2755466A4 (en) 2011-09-13 2015-04-15 Monsanto Technology Llc METHODS AND COMPOSITIONS FOR CONTROLLING WEEDS
AU2012308753B2 (en) * 2011-09-13 2018-05-17 Monsanto Technology Llc Methods and compositions for weed control
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
WO2013039990A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control
CA2848680C (en) 2011-09-13 2020-05-19 Monsanto Technology Llc Methods and compositions for weed control
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
UA116092C2 (uk) 2011-09-13 2018-02-12 Монсанто Текнолоджи Ллс Спосіб та композиція для боротьби з бур'янами (варіанти)
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
CN103827112A (zh) 2011-09-15 2014-05-28 拜耳知识产权有限责任公司 作为杀真菌剂的哌啶吡唑
UA113967C2 (xx) 2011-09-16 2017-04-10 Застосування 5-феніл- або 5-бензил-2-ізоксазолін-3-карбоксилатів для покращення урожайності рослин
BR112014005990B1 (pt) 2011-09-16 2019-12-31 Bayer Ip Gmbh método para induzir uma resposta específica de regulação do crescimento de plantas
BR112014006217B1 (pt) 2011-09-16 2019-01-15 Bayer Intellectual Property Gmbh utilização de acilsulfonamidas para melhorar o rendimento de plantas,método para induzir respostas de regulação de crescimento em plantas úteis ou plantas de cultura e composição.
WO2013041602A1 (de) 2011-09-23 2013-03-28 Bayer Intellectual Property Gmbh Verwendung 4-substituierter 1-phenyl-pyrazol-3-carbonsäurederivate als wirkstoffe gegen abiotischen pflanzenstress
WO2013050410A1 (en) 2011-10-04 2013-04-11 Bayer Intellectual Property Gmbh RNAi FOR THE CONTROL OF FUNGI AND OOMYCETES BY INHIBITING SACCHAROPINE DEHYDROGENASE GENE
WO2013050324A1 (de) 2011-10-06 2013-04-11 Bayer Intellectual Property Gmbh Abiotischen pflanzenstress-reduzierende kombination enthaltend 4- phenylbuttersäure (4-pba) oder eines ihrer salze (komponente (a)) und eine oder mehrere ausgewählte weitere agronomisch wirksame verbindungen (komponente(n) (b)
JP2014534816A (ja) 2011-11-02 2014-12-25 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ 除草剤耐性の増大した植物
EP2782920B1 (en) 2011-11-21 2016-12-21 Bayer Intellectual Property GmbH Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives
CA2856711A1 (en) 2011-11-25 2013-05-30 Bayer Intellectual Property Gmbh Novel heterocyclic alkanol-derivatives
EA201491036A1 (ru) 2011-11-25 2014-11-28 Байер Интеллектуэль Проперти Гмбх Производные 2-йодимидазола
AR089656A1 (es) 2011-11-30 2014-09-10 Bayer Ip Gmbh Derivados de n-bicicloalquil- y n-tricicloalquil-(tio)-carboxamida fungicidas
EP2601839A1 (en) 2011-12-08 2013-06-12 Bayer CropScience AG Synergisitic fungicidal combinations containing phosphorous acid derivative and zoxamide
EP2606732A1 (en) 2011-12-19 2013-06-26 Bayer CropScience AG Use of an anthranilic diamide derivatives with heteroaromatic and heterocyclic substituents in combination with a biological control agent
CN104270946B (zh) 2011-12-19 2017-05-10 拜耳农作物科学股份公司 邻氨基苯甲酸二酰胺衍生物用于防治转基因作物中的害虫的用途
JP5976837B2 (ja) 2011-12-29 2016-08-24 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ 殺菌性３−［（１，３−チアゾール−４−イルメトキシイミノ）（フェニル）メチル］−２−置換−１，２，４−オキサジアゾール−５（２ｈ）−オン誘導体
KR102028903B1 (ko) 2011-12-29 2019-10-07 바이엘 인텔렉쳐 프로퍼티 게엠베하 살진균 3-[(피리딘-2-일메톡시이미노)(페닐)메틸]-2-치환-1,2,4-옥사디아졸-5(2h)-온 유도체
CN108192901A (zh) 2012-02-01 2018-06-22 陶氏益农公司 人造叶绿体转运肽
HUE036328T2 (hu) 2012-02-22 2018-06-28 Bayer Cropscience Ag Fluopirám alkalmazása fabetegségek leküzdésére szõlõn
PE20190343A1 (es) 2012-02-27 2019-03-07 Bayer Ip Gmbh Combinaciones de compuestos activos
TW201400452A (zh) 2012-03-14 2014-01-01 拜耳智慧財產有限公司 除害之芳基吡咯啶類
WO2013139949A1 (en) 2012-03-23 2013-09-26 Bayer Intellectual Property Gmbh Compositions comprising a strigolactame compound for enhanced plant growth and yield
CA2867504A1 (en) 2012-03-29 2013-10-03 Basf Se Co-crystals of dicamba and a co-crystal former b
JP2015517996A (ja) 2012-04-12 2015-06-25 バイエル・クロップサイエンス・アーゲーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ａｇ 殺真菌剤として有用なｎ−アシル−２−（シクロ）アルキルピロリジンおよびピペリジン
JP6109295B2 (ja) 2012-04-20 2017-04-05 バイエル・クロップサイエンス・アクチェンゲゼルシャフト Ｎ−シクロアルキル−ｎ−［（ヘテロシクリルフェニル）メチレン］−（チオ）カルボキサミド誘導体
WO2013156560A1 (en) 2012-04-20 2013-10-24 Bayer Cropscience Ag N-cycloalkyl-n-[(trisubstitutedsilylphenyl)methylene]-(thio)carboxamide derivatives
AU2013254857B2 (en) 2012-04-23 2018-04-26 Bayer Cropscience Nv Targeted genome engineering in plants
JP6342385B2 (ja) 2012-04-26 2018-06-13 アディッソ・フランス・エス．エー．エス．Ａｄｉｓｓｅｏ Ｆｒａｎｃｅ Ｓ．Ａ．Ｓ． ２，４−ジヒドロキシ酪酸を生成する方法
JP6262208B2 (ja) 2012-05-09 2018-01-17 バイエル・クロップサイエンス・アクチェンゲゼルシャフト ピラゾールインダニルカルボキサミド類
US9375005B2 (en) 2012-05-09 2016-06-28 Bayer Cropscience Ag 5-halogenopyrazole indanyl carboxamides
EP2662360A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole indanyl carboxamides
EP2662363A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole biphenylcarboxamides
EP2662361A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazol indanyl carboxamides
EP2662364A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazole tetrahydronaphthyl carboxamides
EP2662362A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazole indanyl carboxamides
EP2662370A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole benzofuranyl carboxamides
AR091104A1 (es) 2012-05-22 2015-01-14 Bayer Cropscience Ag Combinaciones de compuestos activos que comprenden un derivado lipo-quitooligosacarido y un compuesto nematicida, insecticida o fungicida
US10240162B2 (en) 2012-05-24 2019-03-26 A.B. Seeds Ltd. Compositions and methods for silencing gene expression
EP2859104B1 (en) 2012-06-07 2017-05-31 Dow AgroSciences LLC Construct and method for expressing transgenes using a brassica bidirectional constitutive promoter
WO2013188291A2 (en) 2012-06-15 2013-12-19 E. I. Du Pont De Nemours And Company Methods and compositions involving als variants with native substrate preference
AU2013289301A1 (en) 2012-07-11 2015-01-22 Bayer Cropscience Ag Use of fungicidal combinations for increasing the tolerance of a plant towards abiotic stress
JP6293746B2 (ja) 2012-07-11 2018-03-14 アディセオ・フランセ・エス・アー・エス ２，４−ジヒドロキシ酪酸塩の調製のための方法
US9605283B2 (en) 2012-07-11 2017-03-28 Institut National Des Sciences Appliquées Microorganism modified for the production of 1,3-propanediol
EP2687605A1 (en) 2012-07-19 2014-01-22 Biogemma Method for performing homologous recombination
WO2014037340A1 (de) 2012-09-05 2014-03-13 Bayer Cropscience Ag Verwendung substituierter 2-amidobenzimidazole, 2-amidobenzoxazole und 2-amidobenzothiazole oder deren salze als wirkstoffe gegen abiotischen pflanzenstress
CN104736699B (zh) 2012-09-14 2020-04-14 拜尔作物科学有限合伙公司 Hppd变体及使用方法
MX354745B (es) 2012-09-25 2018-03-16 Bayer Cropscience Ag 3-fenilisoxazolin-5-carboxamidas 5-oxi-sustituidas y 3-fenilisoxazolin-5-tioamidas 5-oxi-sustituidas herbicidas y fungicidas.
US20150250174A1 (en) 2012-10-01 2015-09-10 Basf Se Use of n-thio-anthranilamide compounds on cultivated plants
EP2908620A4 (en) 2012-10-18 2016-07-27 Monsanto Technology Llc METHODS AND COMPOSITIONS FOR CONTROLLING PHYTOPARASITES
KR102134565B1 (ko) 2012-10-19 2020-07-16 바이엘 크롭사이언스 악티엔게젤샤프트 카르복사미드 또는 티오카르복사미드 유도체를 사용한 살진균제에 내성을 가지는 진균에 대항하는 식물의 처리 방법
CA2888600C (en) 2012-10-19 2021-08-10 Bayer Cropscience Ag Active compound combinations comprising carboxamide derivatives
KR102123218B1 (ko) 2012-10-19 2020-06-16 바이엘 크롭사이언스 악티엔게젤샤프트 카르복사미드 유도체 및 생물학적 제어제를 포함하는 활성 화합물 조합물
WO2014060519A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives
US20150259294A1 (en) 2012-10-19 2015-09-17 Bayer Cropscience Ag Method of plant growth promotion using carboxamide derivatives
WO2014079820A1 (en) 2012-11-22 2014-05-30 Basf Se Use of anthranilamide compounds for reducing insect-vectored viral infections
WO2014079957A1 (de) 2012-11-23 2014-05-30 Bayer Cropscience Ag Selektive inhibition der ethylensignaltransduktion
EP2735231A1 (en) 2012-11-23 2014-05-28 Bayer CropScience AG Active compound combinations
WO2014083033A1 (en) 2012-11-30 2014-06-05 Bayer Cropsience Ag Binary fungicidal or pesticidal mixture
JP6367215B2 (ja) 2012-11-30 2018-08-01 バイエル・クロップサイエンス・アクチェンゲゼルシャフト 二成分殺菌剤混合物
US9775351B2 (en) 2012-11-30 2017-10-03 Bayer Cropscience Ag Ternary fungicidal and pesticidal mixtures
UA117819C2 (uk) 2012-11-30 2018-10-10 Байєр Кропсайєнс Акцієнгезелльшафт Подвійні пестицидні і фунгіцидні суміші
US9943082B2 (en) 2012-11-30 2018-04-17 Bayer Cropscience Ag Ternary fungicidal mixtures
EP2740720A1 (de) 2012-12-05 2014-06-11 Bayer CropScience AG Substituierte bicyclische- und tricyclische Pent-2-en-4-insäure -Derivate und ihre Verwendung zur Steigerung der Stresstoleranz in Pflanzen
EP2740356A1 (de) 2012-12-05 2014-06-11 Bayer CropScience AG Substituierte (2Z)-5(1-Hydroxycyclohexyl)pent-2-en-4-insäure-Derivate
EP2928296A1 (de) 2012-12-05 2015-10-14 Bayer CropScience AG Verwendung substituierter 1-(arylethinyl)-, 1-(heteroarylethinyl)-, 1-(heterocyclylethinyl)- und 1-(cyloalkenylethinyl)-cyclohexanole als wirkstoffe gegen abiotischen pflanzenstress
WO2014090765A1 (en) 2012-12-12 2014-06-19 Bayer Cropscience Ag Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops
US20140173781A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic wheat plants
AR093996A1 (es) 2012-12-18 2015-07-01 Bayer Cropscience Ag Combinaciones bactericidas y fungicidas binarias
CN104995174A (zh) 2012-12-19 2015-10-21 拜耳作物科学股份公司 二氟甲基-烟酰-四氢萘基胺
EP2935593A2 (en) 2012-12-21 2015-10-28 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
CN105358695B (zh) 2013-01-01 2019-07-12 A.B.种子有限公司 将dsRNA引入植物种子以调节基因表达的方法
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
JP2016516011A (ja) 2013-03-07 2016-06-02 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ ピリメタニル及び選択されたジチインテトラカルボキシイミドの共結晶
WO2014135608A1 (en) 2013-03-07 2014-09-12 Bayer Cropscience Ag Fungicidal 3-{phenyl[(heterocyclylmethoxy)imino]methyl}-heterocycle derivatives
WO2014138339A2 (en) 2013-03-07 2014-09-12 Athenix Corp. Toxin genes and methods for their use
US10612019B2 (en) 2013-03-13 2020-04-07 Monsanto Technology Llc Methods and compositions for weed control
US10609930B2 (en) 2013-03-13 2020-04-07 Monsanto Technology Llc Methods and compositions for weed control
WO2014159306A1 (en) 2013-03-13 2014-10-02 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in brassica
AU2014236154A1 (en) 2013-03-14 2015-09-17 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
US20160040149A1 (en) 2013-03-14 2016-02-11 Pioneer Hi-Bred International Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
EP2981614A1 (en) 2013-04-02 2016-02-10 Bayer CropScience NV Targeted genome engineering in eukaryotes
CN105283449A (zh) 2013-04-12 2016-01-27 拜耳作物科学股份公司 新的三唑硫酮衍生物
MX2015014365A (es) 2013-04-12 2015-12-07 Bayer Cropscience Ag Derivados de triazol novedosos.
CN105555135B (zh) 2013-04-19 2018-06-15 拜耳作物科学股份公司 涉及邻苯二甲酰胺衍生物应用的用于改善对转基因植物生产潜能的利用的方法
EP2986117A1 (en) 2013-04-19 2016-02-24 Bayer CropScience Aktiengesellschaft Binary insecticidal or pesticidal mixture
WO2014177514A1 (en) 2013-04-30 2014-11-06 Bayer Cropscience Ag Nematicidal n-substituted phenethylcarboxamides
WO2014177991A2 (en) * 2013-04-30 2014-11-06 Basf Se Plants having increased tolerance to herbicides
EA201592046A1 (ru) 2013-04-30 2016-11-30 Басф Се Растения, обладающие повышенной толерантностью к воздействию гербицидов
TW201507722A (zh) 2013-04-30 2015-03-01 Bayer Cropscience Ag 做為殺線蟲劑及殺體內寄生蟲劑的ｎ－（２－鹵素－２－苯乙基）－羧醯胺類
EP3013802B1 (en) 2013-06-26 2019-08-14 Bayer Cropscience AG N-cycloalkyl-n-[(bicyclylphenyl)methylene]-(thio)carboxamide derivatives
EP3019012A1 (de) 2013-07-09 2016-05-18 Bayer CropScience AG Verwendung ausgewählter pyridoncarboxamide oder deren salzen als wirkstoffe gegen abiotischen pflanzenstress
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
BR112016000555B1 (pt) 2013-07-19 2022-12-27 Monsanto Technology Llc Método para controlar uma infestação da espécie de leptinotarsa em uma planta, composição inseticida e construção de dna recombinante
BR112016002848B8 (pt) 2013-08-12 2022-03-22 Basf Se Construção de ácido nucleico, vetor, método para produzir uma planta transgênica, método para controlar vegetação indesejada, uso do ácido nucleico, combinação útil para controlar ervas e processo para preparar uma combinação útil para o controle de ervas
UA116400C2 (uk) 2013-09-24 2018-03-12 Байєр Кропсайєнс Нв Білок, що має целюлоза:ксилоглюкан ендотрансглюкозилазну активність, та його застосування
BR112016008489A2 (pt) 2013-10-18 2017-10-03 Pioneer Hi Bred Int Sequências de glifosato-n-acetiltransferase (glyat) e métodos de uso
BR112016008036A2 (pt) 2013-10-25 2018-01-16 Pioneer Hi Bred Int método de identificação de uma primeira planta, kit, polinucleotídeo isolado, planta, germoplasma ou grão de soja
UY35812A (es) 2013-11-04 2015-05-29 Dow Agrosciences Llc ?loci de maíz óptimos?.
BR102014027442B1 (pt) 2013-11-04 2022-09-27 Dow Agrosciences Llc Molécula de ácido nucleico recombinante, uso de uma planta de milho, parte de planta de milho ou célula de planta de milho compreendendo a mesma e método para produzir uma célula vegetal transgênica compreendendo um dna de interesse
RU2694950C2 (ru) 2013-11-04 2019-07-18 Монсанто Текнолоджи Ллс Композиции и способы для борьбы с членистоногими паразитами и заражениями вредителями
NZ746567A (en) 2013-11-04 2019-09-27 Dow Agrosciences Llc Optimal soybean loci
CA2932484A1 (en) 2013-12-05 2015-06-11 Bayer Cropscience Aktiengesellschaft N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives
TW201607929A (zh) 2013-12-05 2016-03-01 拜耳作物科學公司 Ｎ－環烷基－ｎ－｛［２－（１－經取代環烷基）苯基］亞甲基｝－（硫代）甲醯胺衍生物
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
TW201527312A (zh) 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（一）
TW201527314A (zh) 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（三）
TW201527313A (zh) 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（二）
TW201527316A (zh) 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（五）
BR112016016337A2 (pt) 2014-01-15 2017-10-03 Monsanto Technology Llc Composição e métodos para controlar crescimento, desenvolvimento ou a capacidade de reprodução de uma planta, e para sensibilizar uma planta para um herbicida inibidor de epsps
TW201538518A (zh) 2014-02-28 2015-10-16 Dow Agrosciences Llc 藉由嵌合基因調控元件所賦予之根部特異性表現
MX2016011745A (es) 2014-03-11 2017-09-01 Bayer Cropscience Lp Variantes de hppd y metodos de uso.
JP2017514457A (ja) 2014-03-11 2017-06-08 バイエル・クロップサイエンス・アクチェンゲゼルシャフト Ｈｐｐｄバリアントおよび使用方法
US11091770B2 (en) 2014-04-01 2021-08-17 Monsanto Technology Llc Compositions and methods for controlling insect pests
CN106795515B (zh) 2014-06-23 2021-06-08 孟山都技术公司 用于经由rna干扰调控基因表达的组合物和方法
EP3161138A4 (en) 2014-06-25 2017-12-06 Monsanto Technology LLC Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
AR101214A1 (es) 2014-07-22 2016-11-30 Bayer Cropscience Ag Ciano-cicloalquilpenta-2,4-dienos, ciano-cicloalquilpent-2-en-4-inas, ciano-heterociclilpenta-2,4-dienos y ciano-heterociclilpent-2-en-4-inas sustituidos como principios activos contra el estrés abiótico de plantas
US10378012B2 (en) 2014-07-29 2019-08-13 Monsanto Technology Llc Compositions and methods for controlling insect pests
EP2979549A1 (en) 2014-07-31 2016-02-03 Basf Se Method for improving the health of a plant
EP3028573A1 (en) 2014-12-05 2016-06-08 Basf Se Use of a triazole fungicide on transgenic plants
WO2016091675A1 (en) 2014-12-12 2016-06-16 Basf Se Method for improving the health of a plant
WO2016091674A1 (en) 2014-12-12 2016-06-16 Basf Se Use of cyclaniliprole on cultivated plants
AR103024A1 (es) 2014-12-18 2017-04-12 Bayer Cropscience Ag Piridoncarboxamidas seleccionadas o sus sales como sustancias activas contra estrés abiótico de las plantas
CN108064288B (zh) 2015-01-22 2021-11-26 孟山都技术公司 用于控制叶甲属的组合物和方法
US11248234B2 (en) 2015-02-11 2022-02-15 Basf Se Herbicide-resistant hydroxyphenylpyruvate dioxygenases
CA2980505A1 (en) 2015-04-07 2016-10-13 Basf Agrochemical Products B.V. Use of an insecticidal carboxamide compound against pests on cultivated plants
US10214510B2 (en) 2015-04-13 2019-02-26 Bayer Cropscience Aktiengesellschaft N-cycloalkyl-N-(biheterocyclylethylene)-(thio)carboxamide derivatives
EP3302053B1 (en) 2015-06-02 2021-03-17 Monsanto Technology LLC Compositions and methods for delivery of a polynucleotide into a plant
WO2016196782A1 (en) 2015-06-03 2016-12-08 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
WO2016205749A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
MX2018003044A (es) 2015-09-11 2018-04-11 Bayer Cropscience Ag Variantes de hppd y metodos de uso.
AU2017212500B9 (en) 2016-01-26 2023-05-11 Monsanto Technology Llc Compositions and methods for controlling insect pests
WO2017128302A1 (zh) * 2016-01-29 2017-08-03 四川天豫兴禾生物科技有限公司 筛选抗hppd抑制剂类除草剂基因的方法及应用
CN105524931A (zh) * 2016-01-29 2016-04-27 四川天豫兴禾生物科技有限公司 筛选抗hppd抑制剂类除草剂基因的方法及应用
CN105505956B (zh) * 2016-02-02 2017-09-08 中国农业科学院北京畜牧兽医研究所 一种紫花苜蓿对羟基苯丙酮酸双加氧酶基因的应用
US11096344B2 (en) 2016-02-05 2021-08-24 Pioneer Hi-Bred International, Inc. Genetic loci associated with brown stem rot resistance in soybean and methods of use
WO2017184727A1 (en) 2016-04-21 2017-10-26 Bayer Cropscience Lp Tal-effector mediated herbicide tolerance
EP3054014A3 (en) 2016-05-10 2016-11-23 BASF Plant Science Company GmbH Use of a fungicide on transgenic plants
EP3269816A1 (en) 2016-07-11 2018-01-17 Kws Saat Se Development of fungal resistant crops by higs (host-induced gene silencing) mediated inhibition of gpi-anchored cell wall protein synthesis
BR112019001764A2 (pt) 2016-07-29 2019-05-07 Bayer Cropscience Ag combinações de compostos ativos e métodos para proteção de material de propagação de plantas
CN109715622A (zh) 2016-09-22 2019-05-03 拜耳作物科学股份公司 新的三唑衍生物及其作为杀真菌剂的用途
US20190211002A1 (en) 2016-09-22 2019-07-11 Bayer Cropscience Aktiengesellschaft Novel triazole derivatives
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
CA3041351A1 (en) 2016-10-26 2018-05-03 Bayer Cropscience Aktiengesellschaft Use of pyraziflumid for controlling sclerotinia spp in seed treatment applications
US11091772B2 (en) 2016-11-23 2021-08-17 BASF Agricultural Solutions Seed US LLC AXMI669 and AXMI991 toxin genes and methods for their use
US20190387661A1 (en) 2016-12-08 2019-12-26 Bayer Cropscience Aktiengesellschaft Use of insecticides for controlling wireworms
EP3332645A1 (de) 2016-12-12 2018-06-13 Bayer Cropscience AG Verwendung substituierter pyrimidindione oder jeweils deren salze als wirkstoffe gegen abiotischen pflanzenstress
WO2018108627A1 (de) 2016-12-12 2018-06-21 Bayer Cropscience Aktiengesellschaft Verwendung substituierter indolinylmethylsulfonamide oder deren salze zur steigerung der stresstoleranz in pflanzen
EP3338552A1 (en) 2016-12-21 2018-06-27 Basf Se Use of a tetrazolinone fungicide on transgenic plants
WO2018119336A1 (en) 2016-12-22 2018-06-28 Athenix Corp. Use of cry14 for the control of nematode pests
WO2018136611A1 (en) 2017-01-18 2018-07-26 Bayer Cropscience Lp Use of bp005 for the control of plant pathogens
EP3571303A1 (en) 2017-01-18 2019-11-27 Basf Agricultural Solutions Seed Us Llc Bp005 toxin gene and methods for its use
EP3354738A1 (en) 2017-01-30 2018-08-01 Kws Saat Se Transgenic maize plant exhibiting increased yield and drought tolerance
US11708565B2 (en) 2017-03-07 2023-07-25 BASF Agricultural Solutions Seesi US LLC HPPD variants and methods of use
US11180770B2 (en) 2017-03-07 2021-11-23 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
WO2018204777A2 (en) 2017-05-05 2018-11-08 The Broad Institute, Inc. Methods for identification and modification of lncrna associated with target genotypes and phenotypes
EP3630734A1 (en) 2017-05-30 2020-04-08 Basf Se Benzamide compounds and their use as herbicides
CA3063306A1 (en) 2017-05-30 2018-12-06 Basf Se Benzamide compounds and their use as herbicides ii
WO2018228986A1 (de) 2017-06-13 2018-12-20 Bayer Aktiengesellschaft Herbizid wirksame 3-phenylisoxazolin-5-carboxamide von tetrahydro- und dihydrofurancarbonsäureamiden
DK3638665T3 (da) 2017-06-13 2021-10-11 Bayer Ag Herbicidt virksomme 3-phenylisoxazolin-5-carboxamider af tetrahydro- og dihydrofurancarboxylsyrer og -estere
AR112112A1 (es) 2017-06-20 2019-09-18 Basf Se Compuestos de benzamida y su uso como herbicidas
AR112342A1 (es) 2017-07-21 2019-10-16 Basf Se Compuestos de benzamida y su uso como herbicidas
WO2019025153A1 (de) 2017-07-31 2019-02-07 Bayer Cropscience Aktiengesellschaft Verwendung von substituierten n-sulfonyl-n'-aryldiaminoalkanen und n-sulfonyl-n'-heteroaryldiaminoalkanen oder deren salzen zur steigerung der stresstoleranz in pflanzen
BR112020003266A2 (pt) 2017-08-17 2020-10-13 Bayer Aktiengesellschaft 3-fenil-5-trifluorometilisoxazolina-5-carboxamidas herbicidamente ativas de ésteres e ácidos ciclopentilcarboxílicos
US11618896B2 (en) 2017-09-21 2023-04-04 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
BR112020008096A2 (pt) 2017-10-24 2020-11-03 Basf Se método para conferir tolerância a um herbicida e planta de soja transgênica
BR112020008092A2 (pt) 2017-10-24 2020-09-15 BASF Agricultural Solutions Seed US LLC método para conferir tolerância a um herbicida e planta de soja transgênica
EP3360417A1 (de) 2017-11-02 2018-08-15 Bayer CropScience Aktiengesellschaft Verwendung von sulfonylindol als herbizid
AR113857A1 (es) 2017-11-29 2020-06-17 Basf Se Compuestos de benzamida y su uso como herbicidas
AR114040A1 (es) 2017-12-22 2020-07-15 Basf Se Compuestos de benzamida y su uso como herbicidas
WO2019122347A1 (en) 2017-12-22 2019-06-27 Basf Se N-(1,2,5-oxadiazol-3-yl)-benzamide compounds and their use as herbicides
EP3508480A1 (en) 2018-01-08 2019-07-10 Basf Se Benzamide compounds and their use as herbicides
WO2019145245A1 (de) 2018-01-25 2019-08-01 Bayer Aktiengesellschaft Herbizid wirksame 3-phenylisoxazolin-5-carboxamide von cyclopentenylcarbonsäurederivaten
WO2019162309A1 (en) 2018-02-21 2019-08-29 Basf Se Benzamide compounds and their use as herbicides
WO2019162308A1 (en) 2018-02-21 2019-08-29 Basf Se Benzamide compounds and their use as herbicides
US10968257B2 (en) 2018-04-03 2021-04-06 The Broad Institute, Inc. Target recognition motifs and uses thereof
CA3100089A1 (en) 2018-05-15 2019-11-21 Bayer Aktiengesellschaft 2-bromo-6-alkoxyphenyl-substituted pyrrolin-2-ones and their use as herbicides
AR115088A1 (es) 2018-05-15 2020-11-25 Bayer Ag Espirociclohexilpirrolin-2-onas y su uso como herbicidas
WO2019219585A1 (de) 2018-05-15 2019-11-21 Bayer Aktiengesellschaft Neue 3-(4-alkinyl-6-alkoxy-2-chlorphenyl)-3-pyrrolin-2-one und deren verwendung als herbizide
WO2019219588A1 (de) 2018-05-15 2019-11-21 Bayer Aktiengesellschaft Speziell substituierte 2-alkyl-6-alkoxyphenyl-3-pyrroliin-2-one und deren verwendung als herbizide
WO2019228788A1 (de) 2018-05-29 2019-12-05 Bayer Aktiengesellschaft 2-brom-6-alkoxyphenyl-substituierte pyrrolin-2-one und deren verwendung als herbizide
WO2019228787A1 (de) 2018-05-29 2019-12-05 Bayer Aktiengesellschaft Speziell substituierte 2-alkyl-6-alkoxyphenyl-3-pyrrolin-2-one und deren verwendung als herbizide
EP3802521A1 (de) 2018-06-04 2021-04-14 Bayer Aktiengesellschaft Herbizid wirksame bizyklische benzoylpyrazole
AU2019309023A1 (en) 2018-07-26 2021-02-18 Bayer Aktiengesellschaft Use of the succinate dehydrogenase inhibitor fluopyram for controlling root rot complex and/or seedling disease complex caused by rhizoctonia solani, fusarium species and pythium species in brassicaceae species
AU2019343723A1 (en) 2018-09-17 2021-04-15 Bayer Aktiengesellschaft Use of the succinate dehydrogenase inhibitor fluopyram for controlling claviceps purpurea and reducing sclerotia in cereals
CN112714614A (zh) 2018-09-17 2021-04-27 拜耳公司 杀真菌剂异氟普仑用于在谷物中防治麦角菌和减少菌核的用途
CA3124110A1 (en) 2018-12-17 2020-06-25 The Broad Institute, Inc. Crispr-associated transposase systems and methods of use thereof
EP3911633B1 (de) 2019-01-14 2022-11-23 Bayer Aktiengesellschaft Herbizide substituierte n-tetrazolylarylcarboxamide
WO2020169509A1 (de) 2019-02-20 2020-08-27 Bayer Aktiengesellschaft Herbizid wirksame 4-(4-trifluormethyl-6-cycloropylpyrazolyl)pyrimidine
CN113631038B (zh) 2019-03-12 2023-06-30 拜耳公司 除草活性的含s环戊烯基羧酸酯的3-苯基异噁唑啉-5-甲酰胺
US20220151230A1 (en) 2019-03-15 2022-05-19 Bayer Aktiengesellschaft Specifically substituted 3-(2-halogen-6-alkyl-4-propinylphenyl)-3-pyrrolin-2-ones and to the use thereof as herbicides
EP3938349A1 (de) 2019-03-15 2022-01-19 Bayer Aktiengesellschaft Speziell substituierte 3-(2-alkoxy-6-alkyl-4-propinylphenyl)-3-pyrrolin-2-one und deren verwendung als herbizide
CA3133194A1 (en) 2019-03-15 2020-09-24 Bayer Aktiengesellschaft 3-(2-brom-4-alkynyl-6-alkoxyphenyl)-substituted 5-spirocyclohexyl-3-pyrrolin-2-ones and their use as herbicides
JP2022524861A (ja) 2019-03-15 2022-05-10 バイエル・アクチエンゲゼルシヤフト 具体的に置換された３－フェニル－５－スピロシクロペンチル－３－ピロリン－２－オン類及び除草剤としてのその使用
EP3938348A1 (de) 2019-03-15 2022-01-19 Bayer Aktiengesellschaft Neue 3-(2-brom-4-alkinyl-6-alkoxyphenyl)-3-pyrrolin-2-one und deren verwendung als herbizide
MX2021014794A (es) 2019-06-03 2022-01-18 Bayer Ag Acidos 1-fenil-5-azinilpirazolil-3-oxialquilicos y su uso para combatir el crecimiento no deseado de plantas.
US20230066946A1 (en) 2019-12-19 2023-03-02 Bayer Aktiengesellschaft 1,5-diphenylpyrazolyl-3-oxyalkyl acids and 1-phenyl-5-thienylpyrazolyl-3-oxyalkyl acids and the use thereof for control of unwanted plant growth
WO2021204667A1 (de) 2020-04-07 2021-10-14 Bayer Aktiengesellschaft Substituierte isophtalsäurediamide
WO2021204669A1 (de) 2020-04-07 2021-10-14 Bayer Aktiengesellschaft Substituierte isophtalsäurediamide
EP4132916B1 (de) 2020-04-07 2024-01-24 Bayer Aktiengesellschaft Substituierte isophtalsäurediamide und ihre verwendung als herbizide
WO2021204665A1 (de) 2020-04-07 2021-10-14 Bayer Aktiengesellschaft Substituierte isophtalsäurediamide
WO2021204884A1 (de) 2020-04-09 2021-10-14 Bayer Aktiengesellschaft 3-(4-alkenyl-phenyl)-3-pyrrolin-2-one und deren verwendung als herbizide
WO2021209486A1 (de) 2020-04-15 2021-10-21 Bayer Aktiengesellschaft Speziell substituierte pyrrolin-2-one und deren verwendung als herbizide
EP4143181A1 (de) 2020-04-29 2023-03-08 Bayer Aktiengesellschaft 1-pyrazinylpyrazolyl-3-oxyalkylsäuren sowie deren derivate und deren verwendung zur bekämpfung unerwünschten pflanzenwachstums
US20230180758A1 (en) 2020-05-27 2023-06-15 Bayer Aktiengesellschaft Substituted pyrroline-2-ones and their use as herbicides
AU2021367046A1 (en) 2020-10-23 2023-06-08 Bayer Aktiengesellschaft 1-(pyridyl)-5-azinylpyrazole derivatives, and their use for control of undesired plant growth
EP4026833A1 (de) 2021-01-12 2022-07-13 Bayer Aktiengesellschaft Herbizid wirksame 2-(het)arylmethylpyrimidine
WO2022253700A1 (de) 2021-06-01 2022-12-08 Bayer Aktiengesellschaft Speziell substituierte pyrrolin-2-one und deren verwendung als herbizide
AU2022296784A1 (en) 2021-06-25 2024-01-18 Bayer Aktiengesellschaft (1,4,5-trisubstituted-1h-pyrazole-3-yl)oxy-2-alkoxy alkyl acids and their derivatives, their salts and their use as herbicidal agents
WO2023274869A1 (de) 2021-06-29 2023-01-05 Bayer Aktiengesellschaft 3-(4-alkenyl-phenyl)-3-pyrrolin-2-one und deren verwendung als herbizide
AR126252A1 (es) 2021-07-08 2023-10-04 Bayer Ag Amidas de ácido benzoico sustituidas
WO2023044364A1 (en) 2021-09-15 2023-03-23 Enko Chem, Inc. Protoporphyrinogen oxidase inhibitors
AU2022400175A1 (en) 2021-12-01 2024-07-18 Bayer Aktiengesellschaft (1,4,5-trisubstituted-1h-pyrazole-3-yl)oxy-2-alkoxythio alkyl acids and derivatives thereof, their salts and their use as herbicidal active agents
WO2023137309A2 (en) 2022-01-14 2023-07-20 Enko Chem, Inc. Protoporphyrinogen oxidase inhibitors
WO2024078871A1 (de) 2022-10-14 2024-04-18 Bayer Aktiengesellschaft 1-pyridyl-5-phenylpyrazolyl-3-oxy- und -3-thioalkylsäuren und derivate und deren verwendung zur bekämpfung unerwünschten pflanzenwachstums
WO2024137438A2 (en) 2022-12-19 2024-06-27 BASF Agricultural Solutions Seed US LLC Insect toxin genes and methods for their use
EP4431609A1 (en) 2023-03-14 2024-09-18 Adisseo France S.A.S. Method for improving 2, 4 dihydroxybutyric acid production and yield

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2673643B1 (fr) * 1991-03-05 1993-05-21 Rhone Poulenc Agrochimie Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide.
FR2673642B1 (fr) * 1991-03-05 1994-08-12 Rhone Poulenc Agrochimie Gene chimere comprenant un promoteur capable de conferer a une plante une tolerance accrue au glyphosate.
DE4305696A1 (de) * 1993-02-25 1994-09-01 Hoechst Ag Nachweisverfahren zur Identifizierung von Inhibitoren
FR2712302B1 (fr) * 1993-11-10 1996-01-05 Rhone Poulenc Agrochimie Eléments promoteurs de gènes chimères de tubuline alpha.
US6087563A (en) 1996-01-29 2000-07-11 Arizona Board Of Regents On Behalf Of The University Of Arizona Cloned arabidopsis p-hydroxyphenyl pyruvic acid dioxygenase DNA

Also Published As

Publication number Publication date
EP0828837B1 (fr) 2008-01-09
PL323679A1 (en) 1998-04-14
CN1192243A (zh) 1998-09-02
BG102131A (en) 1998-07-31
BRPI9608375C8 (pt) 2018-05-29
US6268549B1 (en) 2001-07-31
MX9709310A (es) 1998-02-28
PT828837E (pt) 2008-04-14
KR100431366B1 (ko) 2004-07-16
KR19990022252A (ko) 1999-03-25
FR2734842B1 (fr) 1998-02-27
SI0828837T1 (sl) 2008-04-30
CA2219979A1 (fr) 1996-12-05
WO1996038567A3 (fr) 1997-05-22
EP0828837A2 (fr) 1998-03-18
DE69637402T2 (de) 2009-01-02
WO1996038567A2 (fr) 1996-12-05
PL189955B1 (pl) 2005-10-31
NZ311055A (en) 2000-02-28
CN1206358C (zh) 2005-06-15
DE69637402D1 (de) 2008-02-21
CA2219979C (fr) 2010-12-14
HUP9900450A2 (hu) 1999-05-28
DK0828837T3 (da) 2008-05-19
ATE383431T1 (de) 2008-01-15
TR199701492T1 (xx) 1998-02-21
ES2297840T3 (es) 2008-05-01
AU718982B2 (en) 2000-05-04
SK161597A3 (en) 1998-07-08
AR002169A1 (es) 1998-01-07
BRPI9608375B8 (pt) 2018-02-27
BR9608375A (pt) 1999-01-05
CO4520296A1 (es) 1997-10-15
JPH11505729A (ja) 1999-05-25
HUP9900450A3 (en) 2002-07-29
CZ380997A3 (cs) 1998-03-18
MA23884A1 (fr) 1996-12-31
BR9608375B1 (pt) 2009-05-05
FR2734842A1 (fr) 1996-12-06
AU6228696A (en) 1996-12-18

Similar Documents

Publication Publication Date Title
HRP960245A2 (en) Dna sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are tolerant to certain herbicides
MXPA97009310A (en) Dna sequence of a hydroxypenyl-piruvate-dioxygenase gene and obtaining plants containing a gene of hydroxypenyl-piruvate-dioxygenase, tolerants at certain herbici
US7935869B2 (en) Chimeric gene with several herbicide tolerance genes, plant cell and plant resistant to several herbicides
JP5563825B2 (ja) 選択マーカー遺伝子
CZ20013860A3 (cs) Herbicidně rezistentní rostliny
CZ20013859A3 (cs) Herbicidně rezistentní rostliny
EP2687085B1 (en) Acetyl-coa carboxylase herbicide resistant sorghum
US20040006784A1 (en) Methods and compositions for producing plants and microorganisms that express feedback insensitive threonine dehydratase/deaminase
US6603064B1 (en) Nuclear male sterile plants, method of producing same and methods to restore fertility
CN114585731B (zh) 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途
AU760662B2 (en) DNA sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are tolerant to certain herbicides
Hnatiuk et al. Creation of winter rapeseed Brassica napus L. commercial line of biotechnological plants, resistant to the glyphosate action
FR2734840A1 (fr) Gene de l&#39;hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant ce gene resistantes aux herbicides
FR2734841A1 (fr) Gene de l&#39;hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant ce gene resistantes aux herbicides
MXPA99000639A (en) Chemical gene for various genes of herbicide tolerance, cell vegetable plants tolerantesa various herbici
HU227099B1 (en) Amelioration of stress tolerance of plants

Legal Events

Date Code Title Description
A1OB Publication of a patent application
AIPI Request for the grant of a patent on the basis of a substantive examination of a patent application
ODBI Application refused