ES8608579A1 - Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular - Google Patents

Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular

Info

Publication number
ES8608579A1
ES8608579A1 ES534102A ES534102A ES8608579A1 ES 8608579 A1 ES8608579 A1 ES 8608579A1 ES 534102 A ES534102 A ES 534102A ES 534102 A ES534102 A ES 534102A ES 8608579 A1 ES8608579 A1 ES 8608579A1
Authority
ES
Spain
Prior art keywords
industrial
strains
expression
interest
molecular cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES534102A
Other languages
English (en)
Spanish (es)
Other versions
ES534102A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8190976&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES8608579(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Publication of ES534102A0 publication Critical patent/ES534102A0/es
Publication of ES8608579A1 publication Critical patent/ES8608579A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
ES534102A 1983-07-06 1984-07-06 Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular Expired ES8608579A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP83201016 1983-07-06

Publications (2)

Publication Number Publication Date
ES534102A0 ES534102A0 (es) 1986-06-16
ES8608579A1 true ES8608579A1 (es) 1986-06-16

Family

ID=8190976

Family Applications (2)

Application Number Title Priority Date Filing Date
ES534102A Expired ES8608579A1 (es) 1983-07-06 1984-07-06 Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular
ES545256A Expired ES8609467A1 (es) 1983-07-06 1985-07-16 Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES545256A Expired ES8609467A1 (es) 1983-07-06 1985-07-16 Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial

Country Status (11)

Country Link
EP (1) EP0134048B2 (OSRAM)
JP (2) JP2669457B2 (OSRAM)
AU (1) AU570709B2 (OSRAM)
DE (1) DE3480411D1 (OSRAM)
DK (1) DK169996B1 (OSRAM)
ES (2) ES8608579A1 (OSRAM)
FI (1) FI85287C (OSRAM)
GR (1) GR82200B (OSRAM)
IE (1) IE58014B1 (OSRAM)
NZ (1) NZ208806A (OSRAM)
WO (1) WO1985000382A1 (OSRAM)

Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
EP0149241B1 (en) * 1983-12-28 1991-03-27 Agency Of Industrial Science And Technology Dna base sequence containing regions involved in the production and secretion of a protein, recombinant dna including the whole or a part of the dna base sequence, and method of producing proteins by use of the recombinant dna
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
IE58099B1 (en) * 1984-06-06 1993-06-30 Cpc International Inc Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
JPH0616711B2 (ja) * 1985-09-27 1994-03-09 メルシャン株式会社 高温で自律増殖するプラスミド
US5024943A (en) * 1985-11-07 1991-06-18 Gist-Brocades Regulatory region cloning and analysis plasmid for bacillus
DK311186D0 (da) * 1986-06-30 1986-06-30 Novo Industri As Enzymer
BR8805647A (pt) * 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
EP0283075B1 (en) * 1987-02-27 1999-05-12 Genencor International, Inc. Molecular cloning and expression of genes encoding proteolytic enzymes
BR8805646A (pt) * 1987-02-27 1989-10-17 Gist Brocades Nv Processo para amplificacao de genes estaveis em adn cromossomico de microorganismos procarioticos
DE69032360T2 (de) * 1989-06-29 1998-12-03 Genencor International, Inc., Rochester, N.Y. Mutierte mikrobielle alpha-Amylasen mit erhöhter thermischer, saurer und/oder alkylischer Stabilität
DE4216246A1 (de) * 1992-05-16 1993-11-18 Solvay Enzymes Gmbh & Co Kg Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
DK1319079T3 (da) 2000-09-21 2013-01-07 Basf Se Talaromyces-xylanase
DK1326957T3 (da) 2000-12-07 2004-08-09 Dsm Ip Assets Bv Fremgangsmåde til forhindring eller formindskelse af uklarheder i drikkevarer
BR0116003A (pt) 2000-12-07 2004-03-30 Dsm Ip Assests Bv Hidrolisados de proteìna enriquecidos em peptìdeos tendo um resìduo de prolina de terminal carbóxi
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
DK1495128T3 (da) 2002-03-29 2014-08-11 Genencor Int Forstærket proteinekspression i Bacillus
CA2490020C (en) 2002-06-07 2011-01-04 Dsm Ip Assets B.V. Improved method for the prevention or reduction of haze in beverages
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
WO2004101763A2 (en) 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme lip1
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
PL1954808T3 (pl) 2005-11-28 2014-05-30 Dsm Ip Assets Bv Preparat enzymatyczny dający czysty smak
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
CA2676686C (en) 2007-02-20 2015-12-15 Dsm Ip Assets B.V. Novel sialidase
JP5867984B2 (ja) 2007-03-12 2016-02-24 ダニスコ・ユーエス・インク 修飾プロテアーゼ
DK2126060T3 (en) 2007-03-22 2017-01-16 Dsm Ip Assets Bv Hitherto unknown lysyl oxidases
EP2460823B1 (en) 2007-05-10 2014-02-26 Danisco US Inc. A modified secretion system to increase expression of polypeptides in bacteria
GB0715751D0 (en) * 2007-08-13 2007-09-19 Tmo Renewables Ltd Thermophilic micro-organisms for ethanol production
US8512982B2 (en) 2007-12-21 2013-08-20 Danisco Us Inc. Enhanced protein production in Bacillus
MX2010010310A (es) 2008-03-28 2010-10-08 Danisco Us Inc Metodo para amplificar un locus en una celula bacteriana.
DK2421973T3 (en) 2009-04-24 2018-07-30 Danisco Us Inc PROTEAS WITH MODIFIED PRO AREAS
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
JP5863666B2 (ja) 2009-12-09 2016-02-17 ダニスコ・ユーエス・インク プロテアーゼ変異体を含む組成物及び方法
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
AR080886A1 (es) 2010-04-15 2012-05-16 Danisco Us Inc Composiciones y metodos que comprenden proteasas variantes
DK2566961T3 (en) 2010-05-06 2018-12-03 Danisco Us Inc COMPOSITIONS AND METHODS OF SUBTILISIN VARIETIES
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
CN103764823B (zh) 2011-05-05 2018-05-11 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
MX357386B (es) 2011-05-05 2018-07-06 Procter & Gamble Composiciones y metodos que comprenden variantes de proteasa serina.
CN102296042B (zh) * 2011-08-26 2013-04-24 甘肃农业大学 地衣芽孢杆菌及用其制备凝乳酶冻干粉的方法
BR112014004444A2 (pt) 2011-08-31 2017-03-21 Danisco Us Inc composições e métodos que compreendem uma variante de enzima lipolítica
WO2013096653A1 (en) 2011-12-22 2013-06-27 Danisco Us Inc. Compositions and methods comprising a lipolytic enzyme variant
MX361862B (es) 2012-10-12 2018-12-18 Danisco Us Inc Composiciones y métodos que comprenden variante de enzima lipolítica.
MX382899B (es) 2012-11-05 2025-03-13 Danisco Us Inc Composiciones y métodos que comprenden variantes de proteasa termolisina.
WO2014100018A1 (en) 2012-12-19 2014-06-26 Danisco Us Inc. Novel mannanase, compositions and methods of use thereof
EP3004341B1 (en) 2013-05-29 2017-08-30 Danisco US Inc. Novel metalloproteases
EP3004314B1 (en) 2013-05-29 2018-06-20 Danisco US Inc. Novel metalloproteases
EP3004342B1 (en) 2013-05-29 2023-01-11 Danisco US Inc. Novel metalloproteases
WO2014194034A2 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
MX371497B (es) 2013-07-19 2020-01-31 Danisco Us Inc Composiciones y metodos que comprenden una variante de enzima lipolitica.
EP3653707B1 (en) 2013-09-12 2025-07-16 Danisco US Inc. Compositions and methods comprising lg12-clade protease variants
DK3514230T3 (da) 2013-12-13 2021-11-15 Danisco Us Inc Serinproteaser af bacillus-arter
WO2015089447A1 (en) 2013-12-13 2015-06-18 Danisco Us Inc. Serine proteases of the bacillus gibsonii-clade
CN106068273B (zh) 2013-12-31 2021-01-08 丹尼斯科美国公司 增强的蛋白质表达
CN106170546A (zh) 2014-03-21 2016-11-30 丹尼斯科美国公司 芽孢杆菌属的丝氨酸蛋白酶
DK3207129T3 (da) 2014-10-17 2020-02-24 Danisco Us Inc Serinproteaser af bacillus-arten
EP4403631A3 (en) 2014-10-27 2024-10-30 Danisco US Inc. Serine proteases
EP3212662B1 (en) 2014-10-27 2020-04-08 Danisco US Inc. Serine proteases
CN107148472A (zh) 2014-10-27 2017-09-08 丹尼斯科美国公司 芽孢杆菌属物种的丝氨酸蛋白酶
EP3212781B1 (en) 2014-10-27 2019-09-18 Danisco US Inc. Serine proteases
EP3957729A1 (en) 2014-10-27 2022-02-23 Danisco US Inc. Serine proteases
KR102588719B1 (ko) 2014-12-16 2023-10-12 다니스코 유에스 인크. 향상된 단백질 발현
US20170369537A1 (en) 2014-12-19 2017-12-28 Danisco Us Inc. Enhanced protein expression
CN107454914B (zh) 2015-03-12 2021-09-21 丹尼斯科美国公司 包含lg12进化枝蛋白酶变体的组合物和方法
JP7274819B2 (ja) 2015-05-13 2023-05-17 ダニスコ・ユーエス・インク ＡｐｒＬ－ＣＬＡＤＥプロテアーゼ変異体及びその使用
CN107922934A (zh) 2015-06-17 2018-04-17 丹尼斯科美国公司 具有经修饰的前肽区域的蛋白酶
EP3310911B1 (en) 2015-06-17 2023-03-15 Danisco US Inc. Bacillus gibsonii-clade serine proteases
BR112018072530A2 (pt) 2016-05-03 2019-03-26 Danisco Us Inc variantes de protease e usos das mesmas
CN109072213A (zh) 2016-05-05 2018-12-21 丹尼斯科美国公司 蛋白酶变体及其用途
MX2018014768A (es) 2016-05-31 2019-03-06 Danisco Us Inc Variantes de proteasa y sus usos.
JP7152319B2 (ja) 2016-06-17 2022-10-12 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
CN110312794B (zh) 2016-12-21 2024-04-12 丹尼斯科美国公司 吉氏芽孢杆菌进化枝丝氨酸蛋白酶
DK3559227T3 (da) 2016-12-21 2025-05-05 Danisco Us Inc Proteasevarianter og anvendelser deraf
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
WO2020046613A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
WO2020242858A1 (en) 2019-05-24 2020-12-03 Danisco Us Inc Subtilisin variants and methods of use
WO2021146255A1 (en) 2020-01-13 2021-07-22 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
WO2022047149A1 (en) 2020-08-27 2022-03-03 Danisco Us Inc Enzymes and enzyme compositions for cleaning
CN117616120A (zh) 2021-06-30 2024-02-27 丹尼斯科美国公司 变体脂肪酶及其用途
WO2023034486A2 (en) 2021-09-03 2023-03-09 Danisco Us Inc. Laundry compositions for cleaning
US20230365897A1 (en) 2021-12-16 2023-11-16 The Procter & Gamble Company Fabric and home care composition including a protease
CN118679251A (zh) 2021-12-16 2024-09-20 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
EP4448712A1 (en) 2021-12-16 2024-10-23 The Procter & Gamble Company Automatic dishwashing composition comprising a protease
EP4448749A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and methods of use
EP4448750A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and uses thereof
CN118974227A (zh) 2022-03-01 2024-11-15 丹尼斯科美国公司 用于清洁的酶和酶组合物
EP4581119A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Detergent compositions and methods related thereto
JP2025529133A (ja) 2022-09-02 2025-09-04 ダニスコ・ユーエス・インク サブチリシン変異体及びそれに関連する方法
CN120303400A (zh) 2022-11-09 2025-07-11 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
EP4410941A1 (en) 2023-02-01 2024-08-07 The Procter & Gamble Company Detergent compositions containing enzymes
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
WO2024186819A1 (en) 2023-03-06 2024-09-12 Danisco Us Inc. Subtilisin variants and methods of use
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
ZA81424B (en) * 1980-02-15 1982-02-24 Cpc International Inc Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
JPS56137896A (en) * 1980-03-10 1981-10-28 Cetus Corp Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria
AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1206108A (en) * 1981-03-06 1986-06-17 Shuichi Aiba Process for transforming microorganism by means of plasmid introduction
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
JPS5889194A (ja) * 1981-11-24 1983-05-27 Ajinomoto Co Inc 微生物によるｌ−トリプトフアンの製造法
JPS58107192A (ja) * 1981-12-18 1983-06-25 Ajinomoto Co Inc 発酵法によるｌ−ヒスチジンの製造法

Also Published As

Publication number Publication date
DK101385D0 (da) 1985-03-05
ES545256A0 (es) 1986-08-01
NZ208806A (en) 1988-07-28
EP0134048B1 (en) 1989-11-08
DK101385A (da) 1985-03-05
JPH06189783A (ja) 1994-07-12
GR82200B (OSRAM) 1984-12-13
DK169996B1 (da) 1995-04-24
ES534102A0 (es) 1986-06-16
JPS60501738A (ja) 1985-10-17
EP0134048A1 (en) 1985-03-13
AU3151084A (en) 1985-02-07
FI85287B (fi) 1991-12-13
FI85287C (fi) 1992-03-25
WO1985000382A1 (en) 1985-01-31
JP2664860B2 (ja) 1997-10-22
EP0134048B2 (en) 1996-08-14
DE3480411D1 (en) 1989-12-14
FI850845L (fi) 1985-03-01
IE841726L (en) 1985-01-06
JP2669457B2 (ja) 1997-10-27
AU570709B2 (en) 1988-03-24
FI850845A0 (fi) 1985-03-01
IE58014B1 (en) 1993-06-16
ES8609467A1 (es) 1986-08-01

Similar Documents

Publication Publication Date Title
ES8609467A1 (es) Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial
ES8603957A1 (es) Procedimiento para obtener una proteina heterologa para levadura
GB2137208B (en) The use of the gal1 promoter
EP0127328A3 (en) The use of chromosomal integration to stabilize heterologous genes
AU557228B2 (en) Method for expressing a gene
DE3471688D1 (en) Dna vectors and their use in recombinant dna technology
JPS57183799A (en) Novel plasmid
AU551251B2 (en) Recombinant dna; chymosin production
EP1170362A3 (en) Cloning and expressing restriction endonucleases and modification methylases from caryophanon
AU551490B2 (en) Dna code for protein to suppress abnormal protein degradation
KR940002352A (ko) 디(d)-아미노산 옥시다제 생산성 형질전환체
DE3380878D1 (en) Method of heterologous cloning of gene in bacillus microorganism
EP0165002A3 (en) Recombinant dna, microorganisms containing same and their use in the production of amylases
DESROCHERS Molecular cloning of a β-xylosidase gene from Bacillus subtilis
WO1986000929A1 (en) Recombinant dna molecule, transformed microorganisms and process for producing penicillin v amidase
EP0323838A3 (de) Expressionsvektor und Verfahren zur regulierbaren Herstellung von Proteinen in Eukaryonten
JPS6486875A (en) Plasmid vector containing streptomycin-sensitive gene
TW373022B (en) Molecular cloning of amylase gene and antibiotics production in streptomyces
JPS5978691A (ja) 高度好熱菌に由来する新規なプラスミド
JPS6437284A (en) Bacillus subtilis having lowered protease productivity
JPS6416586A (en) Production of baniii restriction enzyme

Legal Events

Date Code Title Description
PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20060405