ES8608579A1 - Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular - Google Patents
Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelularInfo
- Publication number
- ES8608579A1 ES8608579A1 ES534102A ES534102A ES8608579A1 ES 8608579 A1 ES8608579 A1 ES 8608579A1 ES 534102 A ES534102 A ES 534102A ES 534102 A ES534102 A ES 534102A ES 8608579 A1 ES8608579 A1 ES 8608579A1
- Authority
- ES
- Spain
- Prior art keywords
- industrial
- strains
- expression
- interest
- molecular cloning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
METODO PARA LA INTRODUCCION DE DNA EN UN ANFITRION DE CAPA DE MICROORGANISMO UNICELULAR, CAPAZ DE MANTENER Y EXPRESAR DE MANERA ESTABLE UN POLIPEPTIDO EN DICHO ANFITRION. COMPRENDE LA COMBINACION, EN PRESENCIA DE UN FUSOGENO, DE PRIMERAS CELULAS ANFITRIONES FACILMENTE TRANSFORMABLES QUE CONTIENEN EL DNA COMO UN ELEMENTO EXTRACROMOSOMAL CAPAZ DE MANTENER EN EL PRIMER ANFITRION, CON CELULAS ANFITRIONES DE CEPA DE MICROORGANISMO UNICELULAR, INDUSTRIAL, RESISTENTE A LA MODIFICACION GENETICA POR UN VECTOR, PLASMIDO, FAGO O COSMIDO, EN CONDICIONES TALES QUE SE PROPORCIONE LA SELECCION DE LAS CELULAS DEL ANFITRION DE CEPA CON LO QUE SE TRANSFIERE A LAS MISMAS EL DNA; Y SELECCION DE LAS CELULAS DEL ANFITRION DE CEPA DE MICROORGANISMO UNICELULAR QUE CONTIENEN DICHO DNA. TIENE APLICACIONES PARA LA PRODUCCION DE POLIPEPTIDOS Y MATERIALES PROTEINICOS ENDOGENOS Y EXOGENOS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP83201016 | 1983-07-06 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8608579A1 true ES8608579A1 (es) | 1986-06-16 |
ES534102A0 ES534102A0 (es) | 1986-06-16 |
Family
ID=8190976
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES534102A Expired ES8608579A1 (es) | 1983-07-06 | 1984-07-06 | Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular |
ES545256A Expired ES8609467A1 (es) | 1983-07-06 | 1985-07-16 | Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES545256A Expired ES8609467A1 (es) | 1983-07-06 | 1985-07-16 | Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0134048B2 (es) |
JP (2) | JP2669457B2 (es) |
AU (1) | AU570709B2 (es) |
DE (1) | DE3480411D1 (es) |
DK (1) | DK169996B1 (es) |
ES (2) | ES8608579A1 (es) |
FI (1) | FI85287C (es) |
GR (1) | GR82200B (es) |
IE (1) | IE58014B1 (es) |
NZ (1) | NZ208806A (es) |
WO (1) | WO1985000382A1 (es) |
Families Citing this family (98)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5624829A (en) * | 1984-07-03 | 1997-04-29 | Gist-Brocades, B.V. | Transformed industrial bacillus strains and methods for making and using them |
CA1311429C (en) * | 1983-07-06 | 1992-12-15 | Vasantha Nagarajan | Vector for polypeptides in bacilli |
DE3484346D1 (de) * | 1983-12-28 | 1991-05-02 | Agency Ind Science Techn | Dns-basensequenz enthaltende regionen einbegriffen in der herstellung und sekretion eines proteins, rekombinante dns das ganze oder einen teil der dns-basensequenz enthaltend und verfahren zur herstellung von proteinen unter verwendung der rekombinanten dns. |
JPS60188093A (ja) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | 枯草菌に於ける形質発現プラスミド |
IE58099B1 (en) * | 1984-06-06 | 1993-06-30 | Cpc International Inc | Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system |
US4801537A (en) * | 1984-06-08 | 1989-01-31 | Genex Corporation | Vector for expression of polypeptides in bacilli |
JPH0616711B2 (ja) * | 1985-09-27 | 1994-03-09 | メルシャン株式会社 | 高温で自律増殖するプラスミド |
US5024943A (en) * | 1985-11-07 | 1991-06-18 | Gist-Brocades | Regulatory region cloning and analysis plasmid for bacillus |
DK311186D0 (da) * | 1986-06-30 | 1986-06-30 | Novo Industri As | Enzymer |
BR8805647A (pt) * | 1987-02-27 | 1989-10-31 | Gist Brocades Nv | Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas |
BR8805646A (pt) * | 1987-02-27 | 1989-10-17 | Gist Brocades Nv | Processo para amplificacao de genes estaveis em adn cromossomico de microorganismos procarioticos |
EP0283075B1 (en) * | 1987-02-27 | 1999-05-12 | Genencor International, Inc. | Molecular cloning and expression of genes encoding proteolytic enzymes |
DE69032360T2 (de) * | 1989-06-29 | 1998-12-03 | Genencor Int | Mutierte mikrobielle alpha-Amylasen mit erhöhter thermischer, saurer und/oder alkylischer Stabilität |
DE4216246A1 (de) * | 1992-05-16 | 1993-11-18 | Solvay Enzymes Gmbh & Co Kg | Einfaches Verfahren zur stabilen chromosomalen Genamplifikation |
GB9907461D0 (en) | 1999-03-31 | 1999-05-26 | King S College London | Neurite regeneration |
US8372620B2 (en) | 1998-12-23 | 2013-02-12 | Dupont Nutrition Biosciences Aps | Bacterial xylanases |
GB0020498D0 (en) | 2000-08-18 | 2000-10-11 | Sterix Ltd | Compound |
JP4878428B2 (ja) | 2000-09-21 | 2012-02-15 | ベーアーエスエフ・アクチエンゲゼルシャフト | タラロマイセスのキシラナーゼ |
TR200401589T4 (tr) | 2000-12-07 | 2004-08-23 | Dsm Ip Assets B.V. | İçeceklerde bulanıklığın engellenmesi ya da azaltılması için yöntem. |
ATE386110T1 (de) | 2000-12-07 | 2008-03-15 | Dsm Ip Assets Bv | Prolylendoprotease aus aspergillus niger |
GB0116453D0 (en) | 2001-07-05 | 2001-08-29 | Imp College Innovations Ltd | Method |
EP3023501A1 (en) | 2002-03-29 | 2016-05-25 | Danisco US Inc. | Enhanced protein expression in bacillus |
JP4713150B2 (ja) | 2002-06-07 | 2011-06-29 | ディーエスエム アイピー アセッツ ビー.ブイ. | 飲料中の濁り防止又は低減の改良方法 |
TWI328612B (en) | 2002-07-25 | 2010-08-11 | Dsm Ip Assets Bv | Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12 |
US20100129862A1 (en) | 2003-05-12 | 2010-05-27 | Jones Brian E | Novel lipolytic Enzyme lip1 |
GB0403864D0 (en) | 2004-02-20 | 2004-03-24 | Ucl Ventures | Modulator |
EP2977453A1 (en) | 2005-11-28 | 2016-01-27 | DSM IP Assets B.V. | Enzyme preparations yielding a clean taste |
GB0600406D0 (en) | 2006-01-10 | 2006-02-15 | Univ Bath | Crystal |
PL2113027T3 (pl) | 2007-02-20 | 2014-11-28 | Dsm Ip Assets Bv | Nowa sialidaza |
RU2496875C2 (ru) | 2007-03-12 | 2013-10-27 | ДАНИСКО ЮЭс ИНК. | Мoдифицированные протеазы |
ES2608033T3 (es) | 2007-03-22 | 2017-04-05 | Dsm Ip Assets B.V. | Nuevas lisil oxidasas |
CN101679489B (zh) | 2007-05-10 | 2014-05-28 | 丹尼斯科美国公司 | 增加细菌内多肽表达的修饰的分泌系统 |
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DK3110833T3 (da) | 2013-05-29 | 2020-04-06 | Danisco Us Inc | Hidtil ukendte metalloproteaser |
ES2956266T3 (es) | 2013-07-19 | 2023-12-18 | Danisco Us Inc | Composiciones y procedimientos que comprenden una variante de enzima lipolítica |
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BR8205954A (pt) * | 1981-10-14 | 1983-09-13 | Unilever Nv | Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos |
JPS5889194A (ja) * | 1981-11-24 | 1983-05-27 | Ajinomoto Co Inc | 微生物によるl−トリプトフアンの製造法 |
JPS58107192A (ja) * | 1981-12-18 | 1983-06-25 | Ajinomoto Co Inc | 発酵法によるl−ヒスチジンの製造法 |
-
1984
- 1984-07-06 GR GR75238A patent/GR82200B/el unknown
- 1984-07-06 IE IE172684A patent/IE58014B1/en not_active IP Right Cessation
- 1984-07-06 ES ES534102A patent/ES8608579A1/es not_active Expired
- 1984-07-06 EP EP19840200990 patent/EP0134048B2/en not_active Expired - Lifetime
- 1984-07-06 JP JP59502729A patent/JP2669457B2/ja not_active Expired - Fee Related
- 1984-07-06 AU AU31510/84A patent/AU570709B2/en not_active Expired
- 1984-07-06 NZ NZ20880684A patent/NZ208806A/xx unknown
- 1984-07-06 WO PCT/NL1984/000021 patent/WO1985000382A1/en active IP Right Grant
- 1984-07-06 DE DE8484200990T patent/DE3480411D1/de not_active Expired
-
1985
- 1985-03-01 FI FI850845A patent/FI85287C/fi not_active IP Right Cessation
- 1985-03-05 DK DK101385A patent/DK169996B1/da not_active IP Right Cessation
- 1985-07-16 ES ES545256A patent/ES8609467A1/es not_active Expired
-
1993
- 1993-10-27 JP JP5269181A patent/JP2664860B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
WO1985000382A1 (en) | 1985-01-31 |
JPH06189783A (ja) | 1994-07-12 |
DE3480411D1 (en) | 1989-12-14 |
GR82200B (es) | 1984-12-13 |
FI850845L (fi) | 1985-03-01 |
NZ208806A (en) | 1988-07-28 |
IE58014B1 (en) | 1993-06-16 |
ES534102A0 (es) | 1986-06-16 |
DK101385A (da) | 1985-03-05 |
FI85287B (fi) | 1991-12-13 |
JP2669457B2 (ja) | 1997-10-27 |
DK101385D0 (da) | 1985-03-05 |
EP0134048A1 (en) | 1985-03-13 |
ES545256A0 (es) | 1986-08-01 |
AU570709B2 (en) | 1988-03-24 |
FI850845A0 (fi) | 1985-03-01 |
ES8609467A1 (es) | 1986-08-01 |
JPS60501738A (ja) | 1985-10-17 |
EP0134048B1 (en) | 1989-11-08 |
JP2664860B2 (ja) | 1997-10-22 |
DK169996B1 (da) | 1995-04-24 |
AU3151084A (en) | 1985-02-07 |
FI85287C (fi) | 1992-03-25 |
IE841726L (en) | 1985-01-06 |
EP0134048B2 (en) | 1996-08-14 |
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Legal Events
Date | Code | Title | Description |
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PC1A | Transfer granted | ||
FD1A | Patent lapsed |
Effective date: 20060405 |