ES8609467A1 - Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial - Google Patents

Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial

Info

Publication number
ES8609467A1
ES8609467A1 ES545256A ES545256A ES8609467A1 ES 8609467 A1 ES8609467 A1 ES 8609467A1 ES 545256 A ES545256 A ES 545256A ES 545256 A ES545256 A ES 545256A ES 8609467 A1 ES8609467 A1 ES 8609467A1
Authority
ES
Spain
Prior art keywords
industrial
strains
expression
interest
molecular cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES545256A
Other languages
English (en)
Other versions
ES545256A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8190976&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES8609467(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Publication of ES545256A0 publication Critical patent/ES545256A0/es
Publication of ES8609467A1 publication Critical patent/ES8609467A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR UN POLIPEPTIDO DE INTERES EN UNA CEPA DE BACILLUS INDUSTRIAL. COMPRENDE: A) PREPARACION DE LA CONSTRUCCION DEL PLASMIDO, INCLUYENDO EL GEN O GENES PARA LOS CUALES SE DESEA LA EXPRESION REALZADA EN EL ANFITRION BACILLUS; B) CLONACION DE LA CONSTRUCCION DE PLASMIDO EN UN ANFITRION COMPATIBLE, QUE SE PUEDE UTILIZAR PARA LA FUSION CON LA CEPA INDUSTRIAL BACILLUS; C) FUSIONAR EL ANFITRION, CONTENIENDO LA CONSTRUCCION DEL PLASMIDO, CON LA CEPA INDUSTRIAL DE BACILLUS; Y D) CRECIMIENTO DE LA CEPA EN UN MEDIO NUTRIENTE ADECUADO PARA LA PRODUCCION DEL PRODUCTO DE EXPRESION DEL GEN O GENES DE INTERES, EN GENERAL UNA ALFA-AMILASA TERMOESTABLE O UNA PROTEASA. SE UTILIZAN EN FERMENTACIONES QUE PRODUCEN POLIPEPTIDOS CON GRAN RENDIMIENTO.
ES545256A 1983-07-06 1985-07-16 Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial Expired ES8609467A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP83201016 1983-07-06

Publications (2)

Publication Number Publication Date
ES545256A0 ES545256A0 (es) 1986-08-01
ES8609467A1 true ES8609467A1 (es) 1986-08-01

Family

ID=8190976

Family Applications (2)

Application Number Title Priority Date Filing Date
ES534102A Expired ES8608579A1 (es) 1983-07-06 1984-07-06 Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular
ES545256A Expired ES8609467A1 (es) 1983-07-06 1985-07-16 Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES534102A Expired ES8608579A1 (es) 1983-07-06 1984-07-06 Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular

Country Status (11)

Country Link
EP (1) EP0134048B2 (es)
JP (2) JP2669457B2 (es)
AU (1) AU570709B2 (es)
DE (1) DE3480411D1 (es)
DK (1) DK169996B1 (es)
ES (2) ES8608579A1 (es)
FI (1) FI85287C (es)
GR (1) GR82200B (es)
IE (1) IE58014B1 (es)
NZ (1) NZ208806A (es)
WO (1) WO1985000382A1 (es)

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Also Published As

Publication number Publication date
WO1985000382A1 (en) 1985-01-31
JPH06189783A (ja) 1994-07-12
DE3480411D1 (en) 1989-12-14
ES8608579A1 (es) 1986-06-16
GR82200B (es) 1984-12-13
FI850845L (fi) 1985-03-01
NZ208806A (en) 1988-07-28
IE58014B1 (en) 1993-06-16
ES534102A0 (es) 1986-06-16
DK101385A (da) 1985-03-05
FI85287B (fi) 1991-12-13
JP2669457B2 (ja) 1997-10-27
DK101385D0 (da) 1985-03-05
EP0134048A1 (en) 1985-03-13
ES545256A0 (es) 1986-08-01
AU570709B2 (en) 1988-03-24
FI850845A0 (fi) 1985-03-01
JPS60501738A (ja) 1985-10-17
EP0134048B1 (en) 1989-11-08
JP2664860B2 (ja) 1997-10-22
DK169996B1 (da) 1995-04-24
AU3151084A (en) 1985-02-07
FI85287C (fi) 1992-03-25
IE841726L (en) 1985-01-06
EP0134048B2 (en) 1996-08-14

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