DE3480411D1 - Molecular cloning and expression in industrial microorganism species - Google Patents

Molecular cloning and expression in industrial microorganism species

Info

Publication number
DE3480411D1
DE3480411D1 DE8484200990T DE3480411T DE3480411D1 DE 3480411 D1 DE3480411 D1 DE 3480411D1 DE 8484200990 T DE8484200990 T DE 8484200990T DE 3480411 T DE3480411 T DE 3480411T DE 3480411 D1 DE3480411 D1 DE 3480411D1
Authority
DE
Germany
Prior art keywords
expression
molecular cloning
microorganism species
industrial microorganism
industrial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8484200990T
Other languages
English (en)
Inventor
Johan Pieter Marinus Sanders
Den Berg Johannes Abel Van
Peter Michael Andreoli
Yvonne Johanna Vos
Ee Jan Hendrik Van
Leo J S M Mulleners
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke DSM NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8190976&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3480411(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Application granted granted Critical
Publication of DE3480411D1 publication Critical patent/DE3480411D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
DE8484200990T 1983-07-06 1984-07-06 Molecular cloning and expression in industrial microorganism species Expired DE3480411D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP83201016 1983-07-06

Publications (1)

Publication Number Publication Date
DE3480411D1 true DE3480411D1 (en) 1989-12-14

Family

ID=8190976

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8484200990T Expired DE3480411D1 (en) 1983-07-06 1984-07-06 Molecular cloning and expression in industrial microorganism species

Country Status (11)

Country Link
EP (1) EP0134048B2 (de)
JP (2) JP2669457B2 (de)
AU (1) AU570709B2 (de)
DE (1) DE3480411D1 (de)
DK (1) DK169996B1 (de)
ES (2) ES8608579A1 (de)
FI (1) FI85287C (de)
GR (1) GR82200B (de)
IE (1) IE58014B1 (de)
NZ (1) NZ208806A (de)
WO (1) WO1985000382A1 (de)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
DE3484346D1 (de) * 1983-12-28 1991-05-02 Agency Ind Science Techn Dns-basensequenz enthaltende regionen einbegriffen in der herstellung und sekretion eines proteins, rekombinante dns das ganze oder einen teil der dns-basensequenz enthaltend und verfahren zur herstellung von proteinen unter verwendung der rekombinanten dns.
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
IE58099B1 (en) * 1984-06-06 1993-06-30 Cpc International Inc Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
JPH0616711B2 (ja) * 1985-09-27 1994-03-09 メルシャン株式会社 高温で自律増殖するプラスミド
US5024943A (en) * 1985-11-07 1991-06-18 Gist-Brocades Regulatory region cloning and analysis plasmid for bacillus
DK311186D0 (da) * 1986-06-30 1986-06-30 Novo Industri As Enzymer
BR8805647A (pt) * 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
BR8805646A (pt) * 1987-02-27 1989-10-17 Gist Brocades Nv Processo para amplificacao de genes estaveis em adn cromossomico de microorganismos procarioticos
EP0283075B1 (de) * 1987-02-27 1999-05-12 Genencor International, Inc. Molekulare Klonierung und Expression von proteolytische Enzyme kodierenden Genen
DE69032360T2 (de) * 1989-06-29 1998-12-03 Genencor Int Mutierte mikrobielle alpha-Amylasen mit erhöhter thermischer, saurer und/oder alkylischer Stabilität
DE4216246A1 (de) * 1992-05-16 1993-11-18 Solvay Enzymes Gmbh & Co Kg Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
JP4878428B2 (ja) 2000-09-21 2012-02-15 ベーアーエスエフ・アクチエンゲゼルシャフト タラロマイセスのキシラナーゼ
TR200401589T4 (tr) 2000-12-07 2004-08-23 Dsm Ip Assets B.V. İçeceklerde bulanıklığın engellenmesi ya da azaltılması için yöntem.
ATE386110T1 (de) 2000-12-07 2008-03-15 Dsm Ip Assets Bv Prolylendoprotease aus aspergillus niger
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
EP3023501A1 (de) 2002-03-29 2016-05-25 Danisco US Inc. Erhöhte expression von subtilisinen in bacillus
JP4713150B2 (ja) 2002-06-07 2011-06-29 ディーエスエム アイピー アセッツ ビー．ブイ． 飲料中の濁り防止又は低減の改良方法
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
US20100129862A1 (en) 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
EP2977453A1 (de) 2005-11-28 2016-01-27 DSM IP Assets B.V. Enzympräparate zur abgabe eines reinen geschmacks
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
PL2113027T3 (pl) 2007-02-20 2014-11-28 Dsm Ip Assets Bv Nowa sialidaza
RU2496875C2 (ru) 2007-03-12 2013-10-27 ДАНИСКО ЮЭс ИНК. Мoдифицированные протеазы
ES2608033T3 (es) 2007-03-22 2017-04-05 Dsm Ip Assets B.V. Nuevas lisil oxidasas
CN101679489B (zh) 2007-05-10 2014-05-28 丹尼斯科美国公司 增加细菌内多肽表达的修饰的分泌系统
GB0715751D0 (en) * 2007-08-13 2007-09-19 Tmo Renewables Ltd Thermophilic micro-organisms for ethanol production
EP2235176B1 (de) 2007-12-21 2017-06-21 Danisco US Inc. Erhöhte proteinproduktion bei beiner bakterie
NZ587540A (en) 2008-03-28 2012-06-29 Danisco Us Inc Method for amplifying locus in bacterial cell
EP2421973B1 (de) 2009-04-24 2018-05-23 Danisco US Inc. Proteasen mit modifizierten pro-bereichen
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
CA2782891C (en) 2009-12-09 2022-01-11 Danisco Us Inc. Compositions and methods comprising protease variants
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
PL2558573T3 (pl) 2010-04-15 2017-08-31 Danisco Us Inc. Kompozycje i sposoby obejmujące warianty proteazy
ES2625751T3 (es) 2010-05-06 2017-07-20 The Procter & Gamble Company Productos de consumo con variantes de proteasa
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
JP5933688B2 (ja) 2011-05-05 2016-06-15 ザ プロクター アンド ギャンブル カンパニー セリンプロテアーゼ変異体を含む組成物及び方法
AR086281A1 (es) 2011-05-05 2013-12-04 Danisco Us Inc Composiciones y metodos que comprenden variantes de serina proteasas
CN102296042B (zh) * 2011-08-26 2013-04-24 甘肃农业大学 地衣芽孢杆菌及用其制备凝乳酶冻干粉的方法
CN103781903A (zh) 2011-08-31 2014-05-07 丹尼斯科美国公司 包含脂肪分解酶变体的组合物和方法
CN104024407A (zh) 2011-12-22 2014-09-03 丹尼斯科美国公司 包含脂解酶变体的组合物和方法
CN104736700B (zh) 2012-10-12 2019-02-01 丹尼斯科美国公司 包含脂解酶变体的组合物和方法
EP2914720B1 (de) 2012-11-05 2022-08-31 Danisco US Inc. Zusammensetzungen und verfahren mit thermolysinproteasevarianten
US20150344858A1 (en) 2012-12-19 2015-12-03 Danisco Us Inc. Novel mannanase, compositions and methods of use thereof
EP3260538B1 (de) 2013-05-29 2021-04-14 Danisco US Inc. Neuartige metalloproteasen
WO2014194032A1 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
EP3004342B1 (de) 2013-05-29 2023-01-11 Danisco US Inc. Neuartige metalloproteasen
DK3110833T3 (da) 2013-05-29 2020-04-06 Danisco Us Inc Hidtil ukendte metalloproteaser
ES2956266T3 (es) 2013-07-19 2023-12-18 Danisco Us Inc Composiciones y procedimientos que comprenden una variante de enzima lipolítica
EP3044313B1 (de) 2013-09-12 2019-11-06 Danisco US Inc. Zusammensetzungen und verfahren mit protease varianten der lg12-klade
DK3080263T3 (da) 2013-12-13 2019-10-07 Danisco Us Inc Serinproteaser af bacillus gibsonii-clade
WO2015089441A1 (en) 2013-12-13 2015-06-18 Danisco Us Inc. Serine proteases of bacillus species
CN106068273B (zh) 2013-12-31 2021-01-08 丹尼斯科美国公司 增强的蛋白质表达
EP3587569B1 (de) 2014-03-21 2022-08-03 Danisco US Inc. Serinproteasen aus einer bacillus-spezies
US20170233710A1 (en) 2014-10-17 2017-08-17 Danisco Us Inc. Serine proteases of bacillus species
EP3224357A1 (de) 2014-10-27 2017-10-04 Danisco US Inc. Serinproteasen aus einer bacillus-spezies
WO2016069569A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
US20170335306A1 (en) 2014-10-27 2017-11-23 Danisco Us Inc. Serine proteases
EP3212783A1 (de) 2014-10-27 2017-09-06 Danisco US Inc. Serinproteasen
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
EP3233893B1 (de) 2014-12-16 2021-01-20 Danisco US Inc. Erhöhte protein expression
EP3234149A1 (de) 2014-12-19 2017-10-25 Danisco US Inc. Erhöhte proteinexpression
US20180216090A1 (en) 2015-03-12 2018-08-02 Danisco Us Inc. Compositions and methods comprising lg12-clade protease variants
JP7274819B2 (ja) 2015-05-13 2023-05-17 ダニスコ・ユーエス・インク ＡｐｒＬ－ＣＬＡＤＥプロテアーゼ変異体及びその使用
CN107922934A (zh) 2015-06-17 2018-04-17 丹尼斯科美国公司 具有经修饰的前肽区域的蛋白酶
EP3310911B1 (de) 2015-06-17 2023-03-15 Danisco US Inc. Bacillus-gibboni-clade-serin-proteasen
BR112018072530A2 (pt) 2016-05-03 2019-03-26 Danisco Us Inc variantes de protease e usos das mesmas
BR112018072586A2 (pt) 2016-05-05 2019-02-19 Danisco Us Inc variantes de protease e usos das mesmas
WO2017210295A1 (en) 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
CA3027745A1 (en) 2016-06-17 2017-12-21 Danisco Us Inc. Protease variants and uses thereof
EP3559226B1 (de) 2016-12-21 2023-01-04 Danisco US Inc. Bacillus-gibboni-clade-serin-proteasen
CN110312795A (zh) 2016-12-21 2019-10-08 丹尼斯科美国公司 蛋白酶变体及其用途
WO2019108599A1 (en) 2017-11-29 2019-06-06 Danisco Us Inc Subtilisin variants having improved stability
US20210214703A1 (en) 2018-06-19 2021-07-15 Danisco Us Inc Subtilisin variants
WO2019245705A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
WO2020046613A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
EP3887515A1 (de) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin-varianten mit verbesserter stabilität
US20220220419A1 (en) 2019-05-24 2022-07-14 Danisco Us Inc Subtilisin variants and methods of use
EP4090727A1 (de) 2020-01-13 2022-11-23 Danisco US Inc. Zusammensetzungen mit einer lipolytischen enzymvariante und verfahren zur verwendung davon
EP4204553A1 (de) 2020-08-27 2023-07-05 Danisco US Inc. Enzyme und enzymzusammensetzungen zur reinigung
WO2023278297A1 (en) 2021-06-30 2023-01-05 Danisco Us Inc Variant lipases and uses thereof
CN117916354A (zh) 2021-09-03 2024-04-19 丹尼斯科美国公司 用于清洁的衣物洗涤组合物
WO2023114932A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114795A1 (en) 2021-12-16 2023-06-22 The Procter & Gamble Company Automatic dishwashing composition comprising a protease
WO2023114794A1 (en) 2021-12-16 2023-06-22 The Procter & Gamble Company Fabric and home care composition comprising a protease
WO2023114936A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
WO2024050343A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Subtilisin variants and methods related thereto
WO2024050346A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Detergent compositions and methods related thereto
WO2024102698A1 (en) 2022-11-09 2024-05-16 Danisco Us Inc. Subtilisin variants and methods of use

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
ZA81424B (en) * 1980-02-15 1982-02-24 Cpc International Inc Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
JPS56137896A (en) * 1980-03-10 1981-10-28 Cetus Corp Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria
AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1206108A (en) * 1981-03-06 1986-06-17 Shuichi Aiba Process for transforming microorganism by means of plasmid introduction
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
JPS5889194A (ja) * 1981-11-24 1983-05-27 Ajinomoto Co Inc 微生物によるｌ−トリプトフアンの製造法
JPS58107192A (ja) * 1981-12-18 1983-06-25 Ajinomoto Co Inc 発酵法によるｌ−ヒスチジンの製造法

Also Published As

Publication number Publication date
WO1985000382A1 (en) 1985-01-31
JPH06189783A (ja) 1994-07-12
ES8608579A1 (es) 1986-06-16
GR82200B (de) 1984-12-13
FI850845L (fi) 1985-03-01
NZ208806A (en) 1988-07-28
IE58014B1 (en) 1993-06-16
ES534102A0 (es) 1986-06-16
DK101385A (da) 1985-03-05
FI85287B (fi) 1991-12-13
JP2669457B2 (ja) 1997-10-27
DK101385D0 (da) 1985-03-05
EP0134048A1 (de) 1985-03-13
ES545256A0 (es) 1986-08-01
AU570709B2 (en) 1988-03-24
FI850845A0 (fi) 1985-03-01
ES8609467A1 (es) 1986-08-01
JPS60501738A (ja) 1985-10-17
EP0134048B1 (de) 1989-11-08
JP2664860B2 (ja) 1997-10-22
DK169996B1 (da) 1995-04-24
AU3151084A (en) 1985-02-07
FI85287C (fi) 1992-03-25
IE841726L (en) 1985-01-06
EP0134048B2 (de) 1996-08-14

Similar Documents

Publication Publication Date Title
DE3480411D1 (en) Molecular cloning and expression in industrial microorganism species
AU551820B2 (en) Copolyester and textile treating composition
EG17596A (en) Disinfection method and composition therefor
GB2178714B (en) Improvements in or relating to multiple station workpiece processing lines
DK539784D0 (da) Hyperproducerende cellulaseproducerende mikroorganisme
AU4876585A (en) Peptide anitbodies and their use in detecting oncogene products
FI840571A0 (fi) Foerfarande foer drivning av en industrirobot i robotkinematikfraemmande koordinater
DE2961090D1 (en) Processing of foodstuffs and composition for the protection of microorganisms used in foodstuff-processing
GB2145110B (en) Alcohol-free wine and its manufacture
YU104684A (en) Tool for manual inoculating in fruit-growing and wine-growing
IL73055A0 (en) Molecular electro-optical transistor and switch
GB2136938B (en) Improvements in furnaces
DE3475417D1 (en) Improvements in and/or relating to cribwalling
ZW17588A1 (en) Microorganism for use in industrial processes
GB9212261D0 (en) Enzyme cloning
GB2165520B (en) Improvements in pallets
EP0174981A4 (de) Behandlungsverfahren für depressionen bei wirbeltieren.
ZA844621B (en) Novel mutant microorganism and its use in removing organic sulfur compounds
IL79701A (en) Clear polyvinyl chloride articles and compositions
EP0169377A3 (en) Plasmid and bacteria containing the same
AU3152284A (en) Einrichtung zur vorreinigung von organisch belasteten abfallsubstraten in geloster und fester formbei gleichzeitiger gewinnung von methangas
GB8719832D0 (en) 2-methyl-5-isopropylhexa-2-5-dien-1-al in microorganisms
GB8428883D0 (en) Bearing arrangement in o-configuration
JPS5786291A (en) Novel bacteria and enzyme
ATE47881T1 (de) Molekulare klonierung und expression in industriellen mikroorganismen.

Legal Events

Date Code Title Description
8363 Opposition against the patent
8366 Restricted maintained after opposition proceedings
8327 Change in the person/name/address of the patent owner

Owner name: DSM N.V., HEERLEN, NL

8310 Action for declaration of annulment
8313 Request for invalidation rejected/withdrawn