DK169996B1 - Fremgangsmåde til effektiv indføring af DNA i en industriel Bacillus-stammevært, fremgangsmåde til fremstilling af et polypeptid i en sådan vært, plasmid, genetisk manipuleret industriel Bacillus-mikroorganisme og anvendelse af en sådan mikroorganisme - Google Patents
Fremgangsmåde til effektiv indføring af DNA i en industriel Bacillus-stammevært, fremgangsmåde til fremstilling af et polypeptid i en sådan vært, plasmid, genetisk manipuleret industriel Bacillus-mikroorganisme og anvendelse af en sådan mikroorganisme Download PDFInfo
- Publication number
- DK169996B1 DK169996B1 DK101385A DK101385A DK169996B1 DK 169996 B1 DK169996 B1 DK 169996B1 DK 101385 A DK101385 A DK 101385A DK 101385 A DK101385 A DK 101385A DK 169996 B1 DK169996 B1 DK 169996B1
- Authority
- DK
- Denmark
- Prior art keywords
- industrial
- bacillus
- host
- dna
- plasmid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP83201016 | 1983-07-06 | ||
| EP83201016 | 1983-07-06 | ||
| PCT/NL1984/000021 WO1985000382A1 (en) | 1983-07-06 | 1984-07-06 | Molecular cloning and expression in industrial microorganism species |
| NL8400021 | 1984-07-06 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK101385A DK101385A (da) | 1985-03-05 |
| DK101385D0 DK101385D0 (da) | 1985-03-05 |
| DK169996B1 true DK169996B1 (da) | 1995-04-24 |
Family
ID=8190976
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK101385A DK169996B1 (da) | 1983-07-06 | 1985-03-05 | Fremgangsmåde til effektiv indføring af DNA i en industriel Bacillus-stammevært, fremgangsmåde til fremstilling af et polypeptid i en sådan vært, plasmid, genetisk manipuleret industriel Bacillus-mikroorganisme og anvendelse af en sådan mikroorganisme |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0134048B2 (de) |
| JP (2) | JP2669457B2 (de) |
| AU (1) | AU570709B2 (de) |
| DE (1) | DE3480411D1 (de) |
| DK (1) | DK169996B1 (de) |
| ES (2) | ES8608579A1 (de) |
| FI (1) | FI85287C (de) |
| GR (1) | GR82200B (de) |
| IE (1) | IE58014B1 (de) |
| NZ (1) | NZ208806A (de) |
| WO (1) | WO1985000382A1 (de) |
Families Citing this family (97)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5624829A (en) * | 1984-07-03 | 1997-04-29 | Gist-Brocades, B.V. | Transformed industrial bacillus strains and methods for making and using them |
| CA1311429C (en) * | 1983-07-06 | 1992-12-15 | Vasantha Nagarajan | Vector for polypeptides in bacilli |
| EP0149241B1 (de) * | 1983-12-28 | 1991-03-27 | Agency Of Industrial Science And Technology | DNS-Basensequenz enthaltende Regionen einbegriffen in der Herstellung und Sekretion eines Proteins, rekombinante DNS das Ganze oder einen Teil der DNS-Basensequenz enthaltend und Verfahren zur Herstellung von Proteinen unter Verwendung der rekombinanten DNS |
| JPS60188093A (ja) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | 枯草菌に於ける形質発現プラスミド |
| IE58099B1 (en) * | 1984-06-06 | 1993-06-30 | Cpc International Inc | Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system |
| US4801537A (en) * | 1984-06-08 | 1989-01-31 | Genex Corporation | Vector for expression of polypeptides in bacilli |
| JPH0616711B2 (ja) * | 1985-09-27 | 1994-03-09 | メルシャン株式会社 | 高温で自律増殖するプラスミド |
| US5024943A (en) * | 1985-11-07 | 1991-06-18 | Gist-Brocades | Regulatory region cloning and analysis plasmid for bacillus |
| DK311186D0 (da) * | 1986-06-30 | 1986-06-30 | Novo Industri As | Enzymer |
| ES2134186T3 (es) * | 1987-02-27 | 1999-10-01 | Genencor Int | Clonaje molecular y expresion de genes que codifican enzimas proteoliticas. |
| RU2091487C1 (ru) * | 1987-02-27 | 1997-09-27 | Гист-Брокейдс Н.В. | Способ получения штамма bacillus, содержащего две копии последовательности днк, кодирующей фермент с гидролазной активностью |
| JP3026567B2 (ja) * | 1987-02-27 | 2000-03-27 | ジェネンコー インターナショナル インコーポレイテッド | 分子のクローニング及びタンパク分解酵素をコードする遺伝子の発現 |
| DK0410498T3 (da) * | 1989-06-29 | 1999-03-22 | Genencor Int | Mutante mikrobielle alfa-amylaser med øget termisk, syre og/eller alkalisk stabilitet |
| DE4216246A1 (de) * | 1992-05-16 | 1993-11-18 | Solvay Enzymes Gmbh & Co Kg | Einfaches Verfahren zur stabilen chromosomalen Genamplifikation |
| GB9907461D0 (en) | 1999-03-31 | 1999-05-26 | King S College London | Neurite regeneration |
| US8372620B2 (en) | 1998-12-23 | 2013-02-12 | Dupont Nutrition Biosciences Aps | Bacterial xylanases |
| GB0020498D0 (en) | 2000-08-18 | 2000-10-11 | Sterix Ltd | Compound |
| CZ2003798A3 (cs) | 2000-09-21 | 2003-08-13 | Dsm N. V. | Polypeptid s aktivitou xylanázy, sekvence nukleové kyseliny kódující polypeptid, způsob přípravy a použití polypeptidu |
| US8119171B2 (en) | 2000-12-07 | 2012-02-21 | Dsm Ip Assets B.V. | Method for the prevention or reduction of haze in beverages |
| ATE386110T1 (de) | 2000-12-07 | 2008-03-15 | Dsm Ip Assets Bv | Prolylendoprotease aus aspergillus niger |
| GB0116453D0 (en) | 2001-07-05 | 2001-08-29 | Imp College Innovations Ltd | Method |
| DK2339016T3 (da) | 2002-03-29 | 2017-01-23 | Danisco Us Inc | Øget produktion af subtilisiner |
| CN100519729C (zh) | 2002-06-07 | 2009-07-29 | Dsmip资产有限公司 | 防止或降低饮料混浊的改进方法 |
| TWI328612B (en) | 2002-07-25 | 2010-08-11 | Dsm Ip Assets Bv | Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12 |
| US20100129862A1 (en) | 2003-05-12 | 2010-05-27 | Jones Brian E | Novel lipolytic Enzyme lip1 |
| GB0403864D0 (en) | 2004-02-20 | 2004-03-24 | Ucl Ventures | Modulator |
| EP2977453A1 (de) | 2005-11-28 | 2016-01-27 | DSM IP Assets B.V. | Enzympräparate zur abgabe eines reinen geschmacks |
| GB0600406D0 (en) | 2006-01-10 | 2006-02-15 | Univ Bath | Crystal |
| WO2008101893A2 (en) | 2007-02-20 | 2008-08-28 | Dsm Ip Assets B.V. | Novel sialidase |
| EP2129779B2 (de) | 2007-03-12 | 2018-12-26 | Danisco US Inc. | Modifizierte proteasen |
| WO2008113799A1 (en) | 2007-03-22 | 2008-09-25 | Dsm Ip Assets B.V. | Novel lysyl oxidases |
| EP2152732B1 (de) | 2007-05-10 | 2012-03-21 | Danisco US Inc. | Modifiziertes ausscheidungssystem zur erhöhung der expression von polypeptiden in bakterien |
| GB0715751D0 (en) * | 2007-08-13 | 2007-09-19 | Tmo Renewables Ltd | Thermophilic micro-organisms for ethanol production |
| KR20100098652A (ko) | 2007-12-21 | 2010-09-08 | 다니스코 유에스 인크. | 바실루스 내에서의 향상된 단백질 생산 |
| JP5659143B2 (ja) | 2008-03-28 | 2015-01-28 | ダニスコ・ユーエス・インク | 細菌性細胞中の遺伝子座を増幅する方法 |
| WO2010123754A1 (en) | 2009-04-24 | 2010-10-28 | Danisco Us Inc. | Proteases with modified pro regions |
| AR076941A1 (es) | 2009-06-11 | 2011-07-20 | Danisco Us Inc | Cepa de bacillus para una mayor produccion de proteina |
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| WO2011101339A2 (en) | 2010-02-18 | 2011-08-25 | Dsm Ip Assets B.V. | Fermentation broth and filtration filter cake and uses thereof |
| CN102858970B (zh) | 2010-04-15 | 2017-07-21 | 丹尼斯科美国公司 | 包含变体蛋白酶的组合物和方法 |
| CA2798451C (en) | 2010-05-06 | 2020-10-06 | Danisco Us Inc. | Compositions and methods comprising subtilisin variants with t022a-e271f substitutions |
| WO2012007446A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Use of a mutarotase in the production of dried powders |
| WO2012007445A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Mutarotase in crystallization |
| EP2705145B1 (de) | 2011-05-05 | 2020-06-17 | The Procter and Gamble Company | Zusammensetzungen und verfahren mit serinproteasevarianten |
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| CN102296042B (zh) * | 2011-08-26 | 2013-04-24 | 甘肃农业大学 | 地衣芽孢杆菌及用其制备凝乳酶冻干粉的方法 |
| EP2751263A1 (de) | 2011-08-31 | 2014-07-09 | Danisco US Inc. | Zusammensetzungen und verfahren mit einer variante eines lipolytischen enzyms |
| BR112014014410A2 (pt) | 2011-12-22 | 2019-09-24 | Danisco Us Inc | composições e métodos que compreendem uma variante de enzima lipolítica |
| CN110093330B (zh) | 2012-10-12 | 2023-11-28 | 丹尼斯科美国公司 | 包含脂解酶变体的组合物和方法 |
| JP6858487B2 (ja) | 2012-11-05 | 2021-04-14 | ダニスコ・ユーエス・インク | サーモリシンプロテアーゼ変異体を含む組成物及び方法 |
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| ES2956266T3 (es) | 2013-07-19 | 2023-12-18 | Danisco Us Inc | Composiciones y procedimientos que comprenden una variante de enzima lipolítica |
| BR112016005286A2 (pt) | 2013-09-12 | 2017-09-12 | Danisco Us Inc | composições e métodos compreendendo variantes de protease do clado lg12 |
| US10533165B2 (en) | 2013-12-13 | 2020-01-14 | Danisco Us Inc | Serine proteases of the bacillus gibsonii-clade |
| EP3080262B1 (de) | 2013-12-13 | 2019-02-06 | Danisco US Inc. | Serinproteaseenzymen von bacillus |
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| CN107278230B (zh) | 2014-12-19 | 2021-10-29 | 丹尼斯科美国公司 | 增强的蛋白质表达 |
| EP3611259A1 (de) | 2015-03-12 | 2020-02-19 | Danisco US Inc. | Zusammensetzungen und verfahren mit lg12-klade-proteasevarianten |
| RU2733987C2 (ru) | 2015-05-13 | 2020-10-09 | ДАНИСКО ЮЭс ИНК. | Варианты протеазы клады aprl и их применения |
| EP3310913A1 (de) | 2015-06-17 | 2018-04-25 | Danisco US Inc. | Proteasen mit modifizierten propeptidregionen |
| EP3310911B1 (de) | 2015-06-17 | 2023-03-15 | Danisco US Inc. | Bacillus-gibboni-clade-serin-proteasen |
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| EP4212622A3 (de) | 2016-12-21 | 2023-11-29 | Danisco US Inc. | Bacillus gibsonii-clade-serinproteasen |
| EP3717643A1 (de) | 2017-11-29 | 2020-10-07 | Danisco US Inc. | Subtilisin-varianten mit verbesserter stabilität |
| US20210363470A1 (en) | 2018-06-19 | 2021-11-25 | Danisco Us Inc | Subtilisin variants |
| EP3810767A1 (de) | 2018-06-19 | 2021-04-28 | Danisco US Inc. | Subtilisin-varianten |
| WO2020046613A1 (en) | 2018-08-30 | 2020-03-05 | Danisco Us Inc | Compositions comprising a lipolytic enzyme variant and methods of use thereof |
| EP3887515A1 (de) | 2018-11-28 | 2021-10-06 | Danisco US Inc. | Subtilisin-varianten mit verbesserter stabilität |
| CN114174504A (zh) | 2019-05-24 | 2022-03-11 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体和使用方法 |
| WO2021146255A1 (en) | 2020-01-13 | 2021-07-22 | Danisco Us Inc | Compositions comprising a lipolytic enzyme variant and methods of use thereof |
| WO2022047149A1 (en) | 2020-08-27 | 2022-03-03 | Danisco Us Inc | Enzymes and enzyme compositions for cleaning |
| EP4363565A1 (de) | 2021-06-30 | 2024-05-08 | Danisco US Inc. | Variante lipasen und verwendungen davon |
| CN117916354A (zh) | 2021-09-03 | 2024-04-19 | 丹尼斯科美国公司 | 用于清洁的衣物洗涤组合物 |
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| NO159863C (no) * | 1980-01-07 | 1989-02-15 | Univ Rochester | Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme. |
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| ZA81424B (en) * | 1980-02-15 | 1982-02-24 | Cpc International Inc | Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same |
| AU545912B2 (en) * | 1980-03-10 | 1985-08-08 | Cetus Corporation | Cloned heterologous jive products in bacillies |
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| CA1206108A (en) * | 1981-03-06 | 1986-06-17 | Shuichi Aiba | Process for transforming microorganism by means of plasmid introduction |
| BR8205954A (pt) * | 1981-10-14 | 1983-09-13 | Unilever Nv | Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos |
| JPS5889194A (ja) * | 1981-11-24 | 1983-05-27 | Ajinomoto Co Inc | 微生物によるｌ−トリプトフアンの製造法 |
| JPS58107192A (ja) * | 1981-12-18 | 1983-06-25 | Ajinomoto Co Inc | 発酵法によるｌ−ヒスチジンの製造法 |
-
1984
- 1984-07-06 IE IE172684A patent/IE58014B1/en not_active IP Right Cessation
- 1984-07-06 DE DE8484200990T patent/DE3480411D1/de not_active Expired
- 1984-07-06 EP EP19840200990 patent/EP0134048B2/de not_active Expired - Lifetime
- 1984-07-06 NZ NZ20880684A patent/NZ208806A/xx unknown
- 1984-07-06 GR GR75238A patent/GR82200B/el unknown
- 1984-07-06 ES ES534102A patent/ES8608579A1/es not_active Expired
- 1984-07-06 WO PCT/NL1984/000021 patent/WO1985000382A1/en active IP Right Grant
- 1984-07-06 AU AU31510/84A patent/AU570709B2/en not_active Expired
- 1984-07-06 JP JP59502729A patent/JP2669457B2/ja not_active Expired - Fee Related
-
1985
- 1985-03-01 FI FI850845A patent/FI85287C/fi not_active IP Right Cessation
- 1985-03-05 DK DK101385A patent/DK169996B1/da not_active IP Right Cessation
- 1985-07-16 ES ES545256A patent/ES8609467A1/es not_active Expired
-
1993
- 1993-10-27 JP JP5269181A patent/JP2664860B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DK101385A (da) | 1985-03-05 |
| ES534102A0 (es) | 1986-06-16 |
| EP0134048B1 (de) | 1989-11-08 |
| JP2669457B2 (ja) | 1997-10-27 |
| FI85287C (fi) | 1992-03-25 |
| FI850845A0 (fi) | 1985-03-01 |
| FI850845L (fi) | 1985-03-01 |
| JPS60501738A (ja) | 1985-10-17 |
| DK101385D0 (da) | 1985-03-05 |
| ES8608579A1 (es) | 1986-06-16 |
| JPH06189783A (ja) | 1994-07-12 |
| ES8609467A1 (es) | 1986-08-01 |
| EP0134048B2 (de) | 1996-08-14 |
| DE3480411D1 (en) | 1989-12-14 |
| JP2664860B2 (ja) | 1997-10-22 |
| AU570709B2 (en) | 1988-03-24 |
| WO1985000382A1 (en) | 1985-01-31 |
| IE58014B1 (en) | 1993-06-16 |
| GR82200B (de) | 1984-12-13 |
| ES545256A0 (es) | 1986-08-01 |
| EP0134048A1 (de) | 1985-03-13 |
| AU3151084A (en) | 1985-02-07 |
| IE841726L (en) | 1985-01-06 |
| FI85287B (fi) | 1991-12-13 |
| NZ208806A (en) | 1988-07-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B1 | Patent granted (law 1993) | ||
| PUP | Patent expired |