DK2339016T3 - Øget produktion af subtilisiner - Google Patents

Øget produktion af subtilisiner Download PDF

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Publication number
DK2339016T3
DK2339016T3 DK11150877.6T DK11150877T DK2339016T3 DK 2339016 T3 DK2339016 T3 DK 2339016T3 DK 11150877 T DK11150877 T DK 11150877T DK 2339016 T3 DK2339016 T3 DK 2339016T3
Authority
DK
Denmark
Prior art keywords
subtilisins
increased production
production
increased
Prior art date
Application number
DK11150877.6T
Other languages
English (en)
Inventor
Eugenio Ferrari
Walter Weyler
Carole Harbison
Harunur Rashid
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Application granted granted Critical
Publication of DK2339016T3 publication Critical patent/DK2339016T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
DK11150877.6T 2002-03-29 2003-03-28 Øget produktion af subtilisiner DK2339016T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US36894902P 2002-03-29 2002-03-29
US36885802P 2002-03-29 2002-03-29
US37634302P 2002-04-29 2002-04-29
EP03726142.7A EP1495128B1 (en) 2002-03-29 2003-03-28 Ehanced protein expression in bacillus

Publications (1)

Publication Number Publication Date
DK2339016T3 true DK2339016T3 (da) 2017-01-23

Family

ID=28678930

Family Applications (2)

Application Number Title Priority Date Filing Date
DK03726142.7T DK1495128T3 (da) 2002-03-29 2003-03-28 Forstærket proteinekspression i Bacillus
DK11150877.6T DK2339016T3 (da) 2002-03-29 2003-03-28 Øget produktion af subtilisiner

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK03726142.7T DK1495128T3 (da) 2002-03-29 2003-03-28 Forstærket proteinekspression i Bacillus

Country Status (7)

Country Link
US (4) US8124399B2 (da)
EP (5) EP2339017A3 (da)
JP (3) JP4680512B2 (da)
AU (1) AU2003228393A1 (da)
CA (2) CA2480277C (da)
DK (2) DK1495128T3 (da)
WO (1) WO2003083125A1 (da)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2339017A3 (en) * 2002-03-29 2011-10-12 Genencor International, Inc. Enhanced protein expression in bacillus
JP4336184B2 (ja) * 2003-11-07 2009-09-30 花王株式会社 組換え微生物
ES2361838T3 (es) 2003-12-03 2011-06-22 Danisco Us Inc. Perhidrolasa.
JP2007532114A (ja) * 2004-04-09 2007-11-15 ジェネンコー・インターナショナル・インク バチルスにおけるｐｃｋＡ修飾および増強されたタンパク質発現
JP2005348641A (ja) * 2004-06-09 2005-12-22 Kao Corp 宿主微生物
CN101321866B (zh) 2005-10-13 2012-11-14 花王株式会社 新型枯草杆菌突变株
DE102006004871A1 (de) 2006-02-02 2007-08-09 Wacker Chemie Ag Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
KR101250826B1 (ko) * 2006-04-24 2013-04-11 아지노모토 가부시키가이샤 퓨린계 물질 생산균 및 퓨린계 물질의 제조법
DK2029732T3 (da) 2006-05-31 2010-02-01 Novozymes As Chloramphenicolresistensselektion i Bacillus licheniformis
RU2496875C2 (ru) * 2007-03-12 2013-10-27 ДАНИСКО ЮЭс ИНК. Мoдифицированные протеазы
CA2704229C (en) * 2007-10-31 2019-05-07 Medimmune, Llc Protein scaffolds comprising seven beta strand domains and six loop regions
DK2257629T3 (da) * 2008-03-28 2016-06-13 Danisco Us Inc Fremgangsmåde til locusamplifikation i en bakteriecelle
WO2011130324A1 (en) 2010-04-13 2011-10-20 Medimmune, Llc Fibronectin type iii domain-based multimeric scaffolds
HUE051439T2 (hu) 2011-10-11 2021-03-01 Viela Bio Inc CD40L-re specifikus, TN3-eredetû állványok és alkalmazásuk
CA2872713A1 (en) 2012-03-13 2013-09-19 Ellen M. Brune Separatome-based protein expression and purification platform
US8945837B2 (en) 2013-03-15 2015-02-03 American Sterilizer Company Sterilization indicator including a simplified genetically engineered biological indicator
EP2796556A1 (en) 2013-04-25 2014-10-29 Rijksuniversiteit Groningen Improved means and methods for expressing recombinant proteins
DK3019607T4 (da) † 2013-07-12 2022-10-17 Novozymes As Direkte overførsel af polynukleotider mellem genomer
US9822371B2 (en) 2013-09-17 2017-11-21 The Board Of Trustees Of The University Of Arkansas E. coli separatome-based protein expression and purification platform
JP2015156844A (ja) * 2014-02-25 2015-09-03 花王株式会社 枯草菌変異株及びそれを用いたジピコリン酸の製造方法
JP6437737B2 (ja) * 2014-05-09 2018-12-12 花王株式会社 枯草菌変異株及びそれを用いたポリ−γ−グルタミン酸の製造方法
JP6527669B2 (ja) * 2014-05-12 2019-06-05 花王株式会社 枯草菌変異株及びそれを用いたジピコリン酸の製造方法
IL295968A (en) 2014-09-17 2022-10-01 Spogen Biotech Inc Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria
US10683528B2 (en) 2014-12-16 2020-06-16 Danisco Us Inc Enhanced protein expression
WO2018053058A1 (en) 2016-09-14 2018-03-22 Danisco Us Inc. Lignocellulosic biomass fermentation-based processes
US11866713B2 (en) 2017-02-24 2024-01-09 Danisco Us Inc. Compositions and methods for increased protein production in bacillus licheniformis
DK3607097T3 (da) 2017-04-07 2023-09-18 Dupont Nutrition Biosci Aps Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet
EP3655537A1 (en) 2017-08-23 2020-05-27 Danisco US Inc. Methods and compositions for efficient genetic modifications of bacillus licheniformis strains
CN111094576A (zh) 2017-09-13 2020-05-01 丹尼斯科美国公司 用于增加芽胞杆菌属中蛋白质产生的经修饰的5′-非翻译区(utr)序列
KR20200105879A (ko) 2018-01-03 2020-09-09 다니스코 유에스 인크. 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법
CN109355303B (zh) * 2018-11-14 2022-05-06 天津大学 抑制和/或敲除基因在提高单克隆抗体的表达量中的应用
US11781147B2 (en) 2018-11-28 2023-10-10 Danisco Us Inc. Promoter sequences and methods thereof for enhanced protein production in Bacillus cells
CN109868253B (zh) * 2019-01-11 2020-03-13 齐鲁工业大学 抑制菌体自溶的地衣芽孢杆菌工程菌及其构建方法和应用
EP4031560A1 (en) 2019-08-14 2022-07-27 Danisco US Inc Compositions and methods for increased protein production in bacillus licheniformis
EP4051793A1 (en) 2019-10-28 2022-09-07 Danisco US Inc Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
CN115335503A (zh) 2019-11-11 2022-11-11 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法
JP2023524334A (ja) 2020-01-15 2023-06-12 ダニスコ・ユーエス・インク バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法
US20240101611A1 (en) 2021-02-22 2024-03-28 Danisco Us Inc. Methods and compositions for producing proteins of interest in pigment deficient bacillus cells
CN117769597A (zh) 2021-05-24 2024-03-26 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法
BR112023027016A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus compreendendo uma proteína reguladora da matriz extracelular a alterada e/ou uma proteína reguladora da matriz extracelular b alterada, método para produzir um composto de interesse, e, proteínas reguladoras das matrizes extracelulares a e b alteradas
EP4359424A1 (en) 2021-06-24 2024-05-01 Basf Se Improved bacillus production host
WO2023023642A2 (en) 2021-08-20 2023-02-23 Danisco Us Inc. Methods and compositions for enhanced protein production in bacillus cells
WO2023091878A1 (en) 2021-11-16 2023-05-25 Danisco Us Inc. Compositions and methods for enhanced protein production in bacillus cells
WO2023183865A1 (en) 2022-03-24 2023-09-28 Danisco Us Inc. Compositions and methods for industrial scale production and recovery of lectins
WO2023192953A1 (en) 2022-04-01 2023-10-05 Danisco Us Inc. Pro-region mutations enhancing protein production in gram-positive bacterial cells
US20230407361A1 (en) * 2022-06-17 2023-12-21 Motif Foodworks, Inc. Inducible cell lysis system
WO2024040043A1 (en) 2022-08-16 2024-02-22 International N&H Denmark Aps Expression systems for phosphatases
WO2024050503A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Novel promoter and 5'-untranslated region mutations enhancing protein production in gram-positive cells
WO2024064653A1 (en) 2022-09-20 2024-03-28 Dupont Nutrition Biosciences Aps Variant anti-viral polypeptides
WO2024091804A1 (en) 2022-10-24 2024-05-02 Danisco Us Inc. Compositions and methods for enhanced protein production in bacillus cells

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302544A (en) 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
US4450235A (en) 1982-04-21 1984-05-22 Cpc International Inc. Asporogenic mutant of bacillus subtilis useful as a host in a host-vector system
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
US5310675A (en) 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
EP0134048B2 (en) 1983-07-06 1996-08-14 Gist-Brocades N.V. Molecular cloning and expression in industrial microorganism species
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5801038A (en) 1984-05-29 1998-09-01 Genencor International Inc. Modified subtilisins having amino acid alterations
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
JP2599946B2 (ja) 1986-01-15 1997-04-16 アムジェン，インコーポレイテツド ズブチリシン類似体
US4980288A (en) 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
GB8608194D0 (en) 1986-04-03 1986-05-08 Massey Ferguson Services Nv Valve control system
DE3772946D1 (de) * 1986-06-19 1991-10-17 Asahi Chemical Ind Heizschrumpfbarer zylindrischer mehrschichtfilm.
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
JP3026567B2 (ja) 1987-02-27 2000-03-27 ジェネンコー インターナショナル インコーポレイテッド 分子のクローニング及びタンパク分解酵素をコードする遺伝子の発現
US4914031A (en) 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
PT89702B (pt) 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
CA2003078A1 (en) * 1988-11-18 1990-05-18 Alan Sloma Protease deletion
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
DE69028890T2 (de) * 1989-08-11 1997-02-27 Gist Brocades Nv Ergiebige Herstellung von Protease-Mutanten
US5585253A (en) * 1989-11-27 1996-12-17 The Regents Of The University Of California Extracellular serine protease and a Bacillus subtilis alkaline neutral an serine protease mutant strain
IE892893A1 (en) 1990-03-11 1991-09-25 Charles O Kane Phage-like bacteriocins
DK97190D0 (da) 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
US5482849A (en) 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DE69230825T2 (de) 1991-04-26 2000-11-30 Genencor Int Genexpression in bazillen
DK0583339T3 (da) 1991-05-01 1999-04-19 Novo Nordisk As Stabiliserede enzymer og detergentsammensætninger
DE4411223A1 (de) 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
WO1999003984A2 (en) * 1997-07-15 1999-01-28 Genencor International, Inc. Proteases from gram-positive organisms
MA25044A1 (fr) 1997-10-23 2000-10-01 Procter & Gamble Compositions de lavage contenant des variants de proteases multisubstituees.
US6528255B1 (en) * 1997-12-30 2003-03-04 Genencor International, Inc. Proteases from gram positive organisms
US6835550B1 (en) 1998-04-15 2004-12-28 Genencor International, Inc. Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
DK1290194T3 (da) * 2000-06-14 2010-09-20 Genencor Int Fremstilling af secernerede polypeptider
EP1309677B2 (en) 2000-08-11 2012-04-11 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
DE10043331A1 (de) 2000-09-02 2002-03-14 Degussa Neue für das sigD-Gen kodierende Nukleotidsequenzen
AU2002243205A1 (en) * 2000-10-10 2002-07-24 Genencor International, Inc. Enhanced secretion of a polypeptide by a microorganism
EP2339017A3 (en) * 2002-03-29 2011-10-12 Genencor International, Inc. Enhanced protein expression in bacillus

Also Published As

Publication number Publication date
EP2339016A3 (en) 2011-11-16
EP1495128B1 (en) 2014-05-07
US8383366B2 (en) 2013-02-26
EP2341070A3 (en) 2011-10-19
AU2003228393A1 (en) 2003-10-13
JP2009225804A (ja) 2009-10-08
US20140011234A1 (en) 2014-01-09
EP2339017A2 (en) 2011-06-29
EP2339016B1 (en) 2015-11-25
US20060073559A1 (en) 2006-04-06
JP5007319B2 (ja) 2012-08-22
US9175294B2 (en) 2015-11-03
EP2339016B8 (en) 2016-01-27
CA2915148A1 (en) 2003-10-09
JP4680512B2 (ja) 2011-05-11
JP2005521407A (ja) 2005-07-21
CA2480277C (en) 2016-02-09
CA2480277A1 (en) 2003-10-09
EP2339016A2 (en) 2011-06-29
WO2003083125A1 (en) 2003-10-09
US20120183995A1 (en) 2012-07-19
US9617549B2 (en) 2017-04-11
JP2011004759A (ja) 2011-01-13
EP1495128A1 (en) 2005-01-12
EP1495128A4 (en) 2006-11-02
EP3023501A1 (en) 2016-05-25
US8124399B2 (en) 2012-02-28
US20160168582A1 (en) 2016-06-16
EP2341070A2 (en) 2011-07-06
EP2339017A3 (en) 2011-10-12
CA2915148C (en) 2018-03-13
DK1495128T3 (da) 2014-08-11
JP5170793B2 (ja) 2013-03-27

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