DK3607097T3 - Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet - Google Patents
Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet Download PDFInfo
- Publication number
- DK3607097T3 DK3607097T3 DK18720841.8T DK18720841T DK3607097T3 DK 3607097 T3 DK3607097 T3 DK 3607097T3 DK 18720841 T DK18720841 T DK 18720841T DK 3607097 T3 DK3607097 T3 DK 3607097T3
- Authority
- DK
- Denmark
- Prior art keywords
- lactases
- galactosidases
- absence
- host cells
- cells producing
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
- A23C9/1206—Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Dairy Products (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762483239P | 2017-04-07 | 2017-04-07 | |
PCT/US2018/026170 WO2018187524A1 (en) | 2017-04-07 | 2018-04-05 | BACILLUS HOST CELLS PRODUCING β-GALACTOSIDASES AND LACTASES IN THE ABSENCE OF P-NITROBENZYLESTERASE SIDE ACTIVITY |
Publications (1)
Publication Number | Publication Date |
---|---|
DK3607097T3 true DK3607097T3 (da) | 2023-09-18 |
Family
ID=62067805
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK18720841.8T DK3607097T3 (da) | 2017-04-07 | 2018-04-05 | Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet |
Country Status (7)
Country | Link |
---|---|
US (1) | US11479763B2 (da) |
EP (1) | EP3607097B1 (da) |
JP (1) | JP7065114B2 (da) |
KR (1) | KR102533803B1 (da) |
DK (1) | DK3607097T3 (da) |
FI (1) | FI3607097T3 (da) |
WO (1) | WO2018187524A1 (da) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR111589A1 (es) * | 2017-04-11 | 2019-07-31 | Chr Hansen As | Enzimas lactasas con propiedades mejoradas |
BR112021007244A2 (pt) * | 2018-10-17 | 2021-08-10 | Chr. Hansen A/S | enzimas lactase com propriedades melhoradas em ph ácido |
AU2019360247A1 (en) * | 2018-10-17 | 2021-06-03 | Perfect Day, Inc. | Recombinant components and compositions for use in food products |
EP3930469A4 (en) * | 2019-02-28 | 2023-03-01 | DuPont Nutrition Biosciences ApS | PROCESS FOR REDUCING LACTOSE AT HIGH TEMPERATURES |
AU2020375621A1 (en) | 2019-10-28 | 2022-04-28 | Danisco Us Inc | Methods and compositions for remediating cyanuric acid in aqueous liquids |
EP4051793A1 (en) | 2019-10-28 | 2022-09-07 | Danisco US Inc | Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases |
CN111411117B (zh) * | 2020-04-27 | 2020-12-01 | 江南大学 | 一种耐热β-葡萄糖苷酶在低聚龙胆糖制备中的应用 |
CN111849940B (zh) * | 2020-07-28 | 2022-03-25 | 量子高科(广东)生物有限公司 | 一种β-半乳糖苷酶的制备方法及其应用 |
WO2024101286A1 (ja) * | 2022-11-11 | 2024-05-16 | 雪印メグミルク株式会社 | N-アセチルラクトサミンの製造方法及びn-アセチルラクトサミン含有組成物 |
Family Cites Families (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2930370B2 (ja) | 1990-05-25 | 1999-08-03 | 雪印乳業株式会社 | ガラクトオリゴ糖類含有脱脂粉乳の製造方法 |
DE69532122T2 (de) * | 1994-06-29 | 2004-07-08 | Dsm Ip Assets B.V. | Erhöhte produktion von beta-galaktosidase in aspergillus oryzae |
RU2225118C2 (ru) | 1998-04-01 | 2004-03-10 | Даниско А/С | Способ изготовления выпечного продукта (варианты), выпечной продукт (варианты), применение немальтогенной экзоамилазы, улучшающая композиция для теста и тесто для выпечного продукта |
US5945325A (en) * | 1998-04-20 | 1999-08-31 | California Institute Of Technology | Thermally stable para-nitrobenzyl esterases |
FR2778921B1 (fr) * | 1998-05-22 | 2001-05-11 | Gervais Danone Sa | Souches mutantes de lactobacillus bulgaricus depourvues d'activite beta-galactosidase |
US6509185B1 (en) | 2000-01-07 | 2003-01-21 | Genencor International, Inc. | Mutant aprE promotor |
EP1283876B1 (en) | 2000-05-26 | 2007-01-24 | Arla Foods amba | Beta-galactosidase isolated from bifidobacterium |
CA2418317A1 (en) | 2000-08-11 | 2002-02-21 | Genencor International, Inc. | Bacillus transformation, transformants and mutant libraries |
AU2003228393A1 (en) * | 2002-03-29 | 2003-10-13 | Genencor International, Inc. | Ehanced protein expression in bacillus |
ATE382367T1 (de) | 2002-04-09 | 2008-01-15 | Univ Missouri | Behandlung des typ 1 diabetes vor und nach expression von prädispositionsmarkern |
EP1576094B1 (en) | 2002-04-22 | 2011-09-28 | Danisco US Inc. | Methods of creating modified promoters resulting in varying levels of gene expression |
EP1953223A1 (en) | 2003-06-13 | 2008-08-06 | Danisco A/S | Variant pseudomonas polypeptides having a non-maltogenic exoamylase activity and their use in preparing food products |
CA2536376A1 (en) | 2003-07-07 | 2005-01-27 | Genencor International, Inc. | Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof |
US8143048B2 (en) | 2003-07-07 | 2012-03-27 | Danisco A/S | Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof |
ES2294958B1 (es) | 2003-07-15 | 2009-02-16 | Mintek | Procedimiento de lixiviacion oxidativa. |
AU2004272629A1 (en) | 2003-09-12 | 2005-03-24 | Commonwealth Scientific And Industrial Research Organisation | Modified gene-silencing nucleic acid molecules and uses thereof |
US20060018997A1 (en) | 2004-07-07 | 2006-01-26 | Kragh Karsten M | Polypeptide |
US20060073583A1 (en) | 2004-09-22 | 2006-04-06 | Kragh Karsten M | Polypeptide |
US20060008890A1 (en) | 2004-07-07 | 2006-01-12 | Kragh Karsten M | Polypeptide |
US20060008888A1 (en) | 2004-07-07 | 2006-01-12 | Kragh Karsten M | Polypeptide |
CA2569381A1 (en) | 2004-07-21 | 2006-08-31 | Ambrx, Inc. | Biosynthetic polypeptides utilizing non-naturally encoded amino acids |
JP2008544751A (ja) | 2005-07-07 | 2008-12-11 | ダニスコ エイ/エス | Pseudomonassaccharophilia由来の修飾アミラーゼ |
FI20065593L (fi) | 2006-09-26 | 2008-03-27 | Valio Oy | Menetelmä galakto-oligosakkarideja sisältävien tuotteiden valmistamiseksi ja niiden käyttö |
WO2009071539A1 (en) | 2007-12-03 | 2009-06-11 | Novozymes A/S | Method for producing a dairy product |
US9107440B2 (en) | 2010-03-29 | 2015-08-18 | Dupont Nutrition Biosciences Aps | Polypeptides having transgalactosylating activity |
MX348325B (es) | 2010-07-19 | 2017-06-06 | Arla Foods Amba | Composicion que contiene galacto-oligosacarido y un metodo para producirla. |
TWI527522B (zh) | 2010-08-13 | 2016-04-01 | 愛之味股份有限公司 | 製備富含半乳寡醣及低乳糖之易吸收乳製品之方法及以該方法製備之機能性乳製品 |
EP2612913B1 (en) * | 2010-08-31 | 2016-07-27 | API Corporation | Novel hydrolase protein |
ES2397334B1 (es) * | 2011-06-24 | 2014-06-06 | Queizúar, S.L. | Cepa de levadura kluyveromyces lactis y procedimiento de obtención de azúcares, etanol, beta-galactosidasa y biomasa. |
CN104053780A (zh) | 2011-12-09 | 2014-09-17 | 丹尼斯科美国公司 | 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 |
JP6641179B2 (ja) | 2012-06-08 | 2020-02-05 | デュポン ニュートリション バイオサイエンシス エーピーエス | トランスガラクトシル化活性を有するポリペプチド |
AU2014363517B2 (en) * | 2013-12-11 | 2018-06-28 | International N&H Denmark Aps | A method for preparing a dairy product having a stable content of galacto-oligosaccharide(s) |
WO2015107050A1 (en) * | 2014-01-14 | 2015-07-23 | Dsm Ip Assets B.V. | Improved enzyme variants of lactase from kluyveromyces lactis |
EP3214942B1 (en) * | 2014-11-07 | 2021-02-24 | DuPont Nutrition Biosciences ApS | Recombinant host cell expressing beta-galactosidase and/or transgalactosylating activity deficient in mannanase, cellulase and pectinase. |
GB201419900D0 (en) | 2014-11-07 | 2014-12-24 | Dupont Nutrition Biosci Aps | Method |
-
2018
- 2018-04-05 WO PCT/US2018/026170 patent/WO2018187524A1/en active Application Filing
- 2018-04-05 DK DK18720841.8T patent/DK3607097T3/da active
- 2018-04-05 EP EP18720841.8A patent/EP3607097B1/en active Active
- 2018-04-05 JP JP2019555016A patent/JP7065114B2/ja active Active
- 2018-04-05 FI FIEP18720841.8T patent/FI3607097T3/fi active
- 2018-04-05 US US16/603,100 patent/US11479763B2/en active Active
- 2018-04-05 KR KR1020197031755A patent/KR102533803B1/ko active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
KR20190138651A (ko) | 2019-12-13 |
EP3607097A1 (en) | 2020-02-12 |
KR102533803B1 (ko) | 2023-05-18 |
JP2020516254A (ja) | 2020-06-11 |
FI3607097T3 (fi) | 2023-09-11 |
EP3607097B1 (en) | 2023-06-21 |
US11479763B2 (en) | 2022-10-25 |
JP7065114B2 (ja) | 2022-05-11 |
WO2018187524A1 (en) | 2018-10-11 |
US20200123519A1 (en) | 2020-04-23 |
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