DK3607097T3 - Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet - Google Patents
Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet Download PDFInfo
- Publication number
- DK3607097T3 DK3607097T3 DK18720841.8T DK18720841T DK3607097T3 DK 3607097 T3 DK3607097 T3 DK 3607097T3 DK 18720841 T DK18720841 T DK 18720841T DK 3607097 T3 DK3607097 T3 DK 3607097T3
- Authority
- DK
- Denmark
- Prior art keywords
- lactases
- galactosidases
- absence
- host cells
- cells producing
- Prior art date
Links
- 102000005936 beta-Galactosidase Human genes 0.000 title 2
- 108010005774 beta-Galactosidase Proteins 0.000 title 2
- 108010054792 4-nitrobenzyl esterase Proteins 0.000 title 1
- 241000193830 Bacillus <bacterium> Species 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
- A23C9/1206—Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Dairy Products (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762483239P | 2017-04-07 | 2017-04-07 | |
| PCT/US2018/026170 WO2018187524A1 (en) | 2017-04-07 | 2018-04-05 | BACILLUS HOST CELLS PRODUCING β-GALACTOSIDASES AND LACTASES IN THE ABSENCE OF P-NITROBENZYLESTERASE SIDE ACTIVITY |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3607097T3 true DK3607097T3 (da) | 2023-09-18 |
Family
ID=62067805
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18720841.8T DK3607097T3 (da) | 2017-04-07 | 2018-04-05 | Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11479763B2 (da) |
| EP (1) | EP3607097B1 (da) |
| JP (1) | JP7065114B2 (da) |
| KR (1) | KR102533803B1 (da) |
| DK (1) | DK3607097T3 (da) |
| FI (1) | FI3607097T3 (da) |
| WO (1) | WO2018187524A1 (da) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110709411A (zh) | 2017-04-11 | 2020-01-17 | 科·汉森有限公司 | 性能改善的乳糖酶 |
| EP4011906A3 (en) | 2017-04-11 | 2022-09-07 | Chr. Hansen A/S | Lactase enzymes with improved properties |
| NZ758427A (en) | 2017-04-11 | 2025-10-31 | Kerry Group Services Int Ltd | Lactase enzymes with improved activity at low temperatures |
| US12435326B2 (en) | 2017-04-11 | 2025-10-07 | Kerry Group Services International Ltd | Lactase enzymes with improved properties |
| US20220033788A1 (en) * | 2018-10-17 | 2022-02-03 | Perfect Day, Inc. | Recombinant components and compositions for use in food products |
| CN112654251A (zh) * | 2018-10-17 | 2021-04-13 | 科·汉森有限公司 | 在酸性pH下具有改善的特性的乳糖酶 |
| EP3930469A4 (en) * | 2019-02-28 | 2023-03-01 | DuPont Nutrition Biosciences ApS | PROCESS FOR REDUCING LACTOSE AT HIGH TEMPERATURES |
| WO2021086606A1 (en) | 2019-10-28 | 2021-05-06 | Danisco Us Inc | Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases |
| EP4051643A1 (en) | 2019-10-28 | 2022-09-07 | Danisco US Inc. | Methods and compositions for remediating cyanuric acid in aqueous liquids |
| CN111411117B (zh) * | 2020-04-27 | 2020-12-01 | 江南大学 | 一种耐热β-葡萄糖苷酶在低聚龙胆糖制备中的应用 |
| CN111849940B (zh) * | 2020-07-28 | 2022-03-25 | 量子高科(广东)生物有限公司 | 一种β-半乳糖苷酶的制备方法及其应用 |
| AU2023376898A1 (en) * | 2022-11-11 | 2025-05-15 | Megmilk Snow Brand Co., Ltd. | N-acetyllactosamine production method and n-acetyllactosamine-containing composition |
| CN120005857B (zh) * | 2023-11-07 | 2025-10-17 | 中国农业大学 | 米曲霉β-半乳糖苷酶突变体的高效表达及在乳品中的应用 |
Family Cites Families (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2930370B2 (ja) | 1990-05-25 | 1999-08-03 | 雪印乳業株式会社 | ガラクトオリゴ糖類含有脱脂粉乳の製造方法 |
| CA2193965C (en) * | 1994-06-29 | 2007-11-13 | Randy M. Berka | Increased production of .beta.-galactosidase in aspergillus oryzae |
| WO1999050399A2 (en) | 1998-04-01 | 1999-10-07 | Danisco A/S | Non-maltogenic exoamylases and their use in retarding retrogradation of starch |
| US5945325A (en) * | 1998-04-20 | 1999-08-31 | California Institute Of Technology | Thermally stable para-nitrobenzyl esterases |
| FR2778921B1 (fr) * | 1998-05-22 | 2001-05-11 | Gervais Danone Sa | Souches mutantes de lactobacillus bulgaricus depourvues d'activite beta-galactosidase |
| US6509185B1 (en) | 2000-01-07 | 2003-01-21 | Genencor International, Inc. | Mutant aprE promotor |
| EP1283876B1 (en) | 2000-05-26 | 2007-01-24 | Arla Foods amba | Beta-galactosidase isolated from bifidobacterium |
| DK1309677T4 (da) | 2000-08-11 | 2012-06-25 | Genencor Int | Bacillustransformation, transformanter og mutantbiblioteker |
| DK2339016T3 (da) | 2002-03-29 | 2017-01-23 | Danisco Us Inc | Øget produktion af subtilisiner |
| EP1499346B1 (en) | 2002-04-09 | 2008-01-02 | The Curators Of The University Of Missouri | Treatment of type 1 diabetes before and after expression of predisposition markers |
| DK1576094T3 (da) | 2002-04-22 | 2012-01-02 | Danisco Us Inc | Fremgangsmåder til at danne modificerede promotere resulterende i varierende genekspressionsniveauer |
| DK1654355T3 (da) | 2003-06-13 | 2010-08-09 | Danisco | Pseudomonas polypeptidvarianter med ikke-maltogen exoamylaseaktivitet og deres anvendelse til fremstilling af fødevarer |
| BRPI0412402A (pt) | 2003-07-07 | 2006-09-05 | Genencor Int | polipeptìdeos de amilase termoestáveis, ácidos nucléicos codificando esses polipeptìdeos e seus usos |
| US8143048B2 (en) | 2003-07-07 | 2012-03-27 | Danisco A/S | Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof |
| CN100417733C (zh) | 2003-07-15 | 2008-09-10 | 明特克公司 | 氧化浸提方法 |
| EP1664298A4 (en) | 2003-09-12 | 2010-03-17 | Commw Scient Ind Res Org | MODIFIED GEN-SILENCING NUCLEIC ACID MOLECULES AND USES THEREOF |
| US20060008890A1 (en) | 2004-07-07 | 2006-01-12 | Kragh Karsten M | Polypeptide |
| US20060073583A1 (en) | 2004-09-22 | 2006-04-06 | Kragh Karsten M | Polypeptide |
| US20060018997A1 (en) | 2004-07-07 | 2006-01-26 | Kragh Karsten M | Polypeptide |
| US20060008888A1 (en) | 2004-07-07 | 2006-01-12 | Kragh Karsten M | Polypeptide |
| EP1771573A4 (en) | 2004-07-21 | 2009-02-18 | Ambrx Inc | BIOSYNTHETIC POLYPEPTIDES OBTAINED FROM NON-NATURALLY CITED AMINO ACIDS |
| WO2007007053A1 (en) | 2005-07-07 | 2007-01-18 | Danisco A/S | Modified amylase from pseudomonas saccharophilia |
| FI20065593A7 (fi) | 2006-09-26 | 2008-03-27 | Valio Oy | Menetelmä galakto-oligosakkarideja sisältävien tuotteiden valmistamiseksi ja niiden käyttö |
| PL2234501T3 (pl) | 2007-12-03 | 2017-08-31 | Novozymes A/S | Sposób otrzymywania produktu mlecznego o niskiej zawartości laktozy |
| DK3392268T3 (da) | 2010-03-29 | 2021-09-27 | Dupont Nutrition Biosci Aps | Polypeptider med transgalactosylerende aktivitet |
| AR082261A1 (es) | 2010-07-19 | 2012-11-21 | Arla Foods Amba | Composicion que contiene galacto-oligosacaridos y un metodo para producirla |
| TWI527522B (zh) | 2010-08-13 | 2016-04-01 | 愛之味股份有限公司 | 製備富含半乳寡醣及低乳糖之易吸收乳製品之方法及以該方法製備之機能性乳製品 |
| SG187652A1 (en) * | 2010-08-31 | 2013-03-28 | Api Corp | Novel hydrolase protein |
| ES2397334B1 (es) * | 2011-06-24 | 2014-06-06 | Queizúar, S.L. | Cepa de levadura kluyveromyces lactis y procedimiento de obtención de azúcares, etanol, beta-galactosidasa y biomasa. |
| DK2788491T3 (da) | 2011-12-09 | 2019-04-01 | Danisco Us Inc | Ribosomale promotorer fra b. subtilis til proteinproduktion i mikroorganismer |
| PL3473714T3 (pl) * | 2012-06-08 | 2022-07-25 | Dupont Nutrition Biosciences Aps | Polipeptydy mające aktywność transgalaktozylacji |
| CN116195628A (zh) * | 2013-12-11 | 2023-06-02 | 杜邦营养生物科学有限公司 | 用于制备具有稳定低聚半乳糖含量的乳制品的方法 |
| WO2015107050A1 (en) | 2014-01-14 | 2015-07-23 | Dsm Ip Assets B.V. | Improved enzyme variants of lactase from kluyveromyces lactis |
| GB201419900D0 (en) | 2014-11-07 | 2014-12-24 | Dupont Nutrition Biosci Aps | Method |
| BR112017009189A2 (pt) * | 2014-11-07 | 2018-03-06 | Dupont Nutrition Biosci Aps | célula hospedeira recombinante expressando atividade de beta-galactosidase e/ou transgalactosilante deficiente em mananase, celulase e pectinase |
-
2018
- 2018-04-05 DK DK18720841.8T patent/DK3607097T3/da active
- 2018-04-05 FI FIEP18720841.8T patent/FI3607097T3/fi active
- 2018-04-05 KR KR1020197031755A patent/KR102533803B1/ko active Active
- 2018-04-05 JP JP2019555016A patent/JP7065114B2/ja active Active
- 2018-04-05 US US16/603,100 patent/US11479763B2/en active Active
- 2018-04-05 WO PCT/US2018/026170 patent/WO2018187524A1/en not_active Ceased
- 2018-04-05 EP EP18720841.8A patent/EP3607097B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2020516254A (ja) | 2020-06-11 |
| EP3607097B1 (en) | 2023-06-21 |
| JP7065114B2 (ja) | 2022-05-11 |
| US20200123519A1 (en) | 2020-04-23 |
| EP3607097A1 (en) | 2020-02-12 |
| WO2018187524A1 (en) | 2018-10-11 |
| FI3607097T3 (fi) | 2023-09-11 |
| US11479763B2 (en) | 2022-10-25 |
| KR20190138651A (ko) | 2019-12-13 |
| KR102533803B1 (ko) | 2023-05-18 |
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