KR910001046A - 비융합 단백질을 제조하기 위한 미생물내 발현용 발현벡터 - Google Patents

비융합 단백질을 제조하기 위한 미생물내 발현용 발현벡터 Download PDF

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Publication number
KR910001046A
KR910001046A KR1019890007840A KR890007840A KR910001046A KR 910001046 A KR910001046 A KR 910001046A KR 1019890007840 A KR1019890007840 A KR 1019890007840A KR 890007840 A KR890007840 A KR 890007840A KR 910001046 A KR910001046 A KR 910001046A
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KR
South Korea
Prior art keywords
expression vector
expression
coli
ptrc
microorganism
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KR1019890007840A
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English (en)
Inventor
아만 에곤
아벨 카를-요세프
Original Assignee
필립 스타인
베링베르케 아크티엔게젤샤프트
헤르베르트 부크
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Application filed by 필립 스타인, 베링베르케 아크티엔게젤샤프트, 헤르베르트 부크 filed Critical 필립 스타인
Publication of KR910001046A publication Critical patent/KR910001046A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon

Abstract

내용 없음.

Description

비융합 단백질을 제조하기 위한 미생물내 발현용 발현벡터
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
도면은 플라즈미드 pTrc 99A. -B. -C의 제조방법을 도시한 것이다.

Claims (8)

  1. a) trc 프로모터 b) lacZ 리보좀-결합부위, c) 해독출발 코돈을 함유하는 NcoI 절단부위, d) 3개의 리딩프레임(reading frame) 모두내의 제한효소 절단부위 폴리링커, 및 e) 플러스 전사 터미네이터를 함유하며, f) 상기 요소들이 복제시작점(origin). 항생제 -내성 유전자 및 lacIg 대립인자를 함유하는 플라즈미드 주쇄(backbone)에 도입되는, 외래성 단백질을 이. 콜라이(E. coli)내에서 발현시키기 위한 발현벡터.
  2. 제1항에 있어서, 플라즈미드 주쇄로서 pBR322 DNA를 지니는 발현벡터.
  3. 제2항에 있어서, 2066 부위 이하에서 pBR322 DNA가 결실된 발현벡터.
  4. 제1항에 있어서, pUC 18에서 유도된 EcoRI-Hind III 폴리링커를 지니는 발현벡터.
  5. 제1항에 있어서, rrnB 오페론의 전사 터미네이터를 갖는 발현벡터.
  6. 플라즈미드 pTrc 99A. pTrc 99B 및 pTrc 99C.
  7. 제1항의 요소 (a) 내지 (f)를 연결시킴을 특징으로 하여, 제1항 내지 6항에 청구된 발현벡터를 제조하는 방법.
  8. 외래성 단백질을 이.콜라이 내에서 발현시키기 위한 제1항 내지 6항에 청구된 발현벡터의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890007840A 1988-06-08 1989-06-08 비융합 단백질을 제조하기 위한 미생물내 발현용 발현벡터 KR910001046A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3819463.5 1988-06-08
DE3819463A DE3819463A1 (de) 1988-06-08 1988-06-08 Expressionsvektoren zur herstellung unfusionierter proteine in mikroorganismen

Publications (1)

Publication Number Publication Date
KR910001046A true KR910001046A (ko) 1991-01-30

Family

ID=6356098

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890007840A KR910001046A (ko) 1988-06-08 1989-06-08 비융합 단백질을 제조하기 위한 미생물내 발현용 발현벡터

Country Status (8)

Country Link
EP (1) EP0345615A3 (ko)
JP (1) JPH0239891A (ko)
KR (1) KR910001046A (ko)
AU (1) AU620873B2 (ko)
DE (1) DE3819463A1 (ko)
DK (1) DK278289A (ko)
FI (1) FI892761A (ko)
PT (1) PT90768A (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8379991A (en) * 1990-09-14 1992-03-26 Astra Aktiebolag A novel method of generating clones for the expression of unfused proteins
WO1994003613A1 (en) * 1992-08-10 1994-02-17 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Dna coding for decarbamylase improved in thermostability and use thereof
SE9203753D0 (sv) * 1992-12-11 1992-12-11 Kabi Pharmacia Ab Expression system for producing apolipoprotein ai-m
JP3088617B2 (ja) * 1994-08-29 2000-09-18 日立建機株式会社 駐車ブレーキの操作装置および駐車ブレーキの解除方法
SE9500778D0 (sv) * 1995-03-03 1995-03-03 Pharmacia Ab Process for producing a protein
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use
WO1997004110A1 (en) * 1995-07-14 1997-02-06 Somatogen, Inc. Methods for increasing protein expression
SE9603068D0 (sv) 1996-08-23 1996-08-23 Pharmacia & Upjohn Ab Process for purifying a protein
SE9603303D0 (sv) 1996-09-11 1996-09-11 Pharmacia & Upjohn Ab Process for purifying a protein

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3621371A1 (de) * 1986-03-12 1987-09-17 Behringwerke Ag Gentechnische herstellung von faktor xiiia

Also Published As

Publication number Publication date
FI892761A0 (fi) 1989-06-06
AU620873B2 (en) 1992-02-27
PT90768A (pt) 1989-12-29
EP0345615A3 (de) 1990-11-22
AU3608589A (en) 1989-12-14
DK278289D0 (da) 1989-06-07
DK278289A (da) 1989-12-09
JPH0239891A (ja) 1990-02-08
FI892761A (fi) 1989-12-09
DE3819463A1 (de) 1989-12-14
EP0345615A2 (de) 1989-12-13

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