KR890000667A - 외래성 dna를 메틸 영양 요구성 세균에서 발현시키기 위한 프로모터, 이의 제조방법 및 이의 용도 - Google Patents

외래성 dna를 메틸 영양 요구성 세균에서 발현시키기 위한 프로모터, 이의 제조방법 및 이의 용도 Download PDF

Info

Publication number
KR890000667A
KR890000667A KR1019880007521A KR880007521A KR890000667A KR 890000667 A KR890000667 A KR 890000667A KR 1019880007521 A KR1019880007521 A KR 1019880007521A KR 880007521 A KR880007521 A KR 880007521A KR 890000667 A KR890000667 A KR 890000667A
Authority
KR
South Korea
Prior art keywords
region
promoter
methyl
methyltrophic
promoters
Prior art date
Application number
KR1019880007521A
Other languages
English (en)
Inventor
빈나커 에른스트-루드비히
메츨러 토마스
마르퀴르트 뤼디거
프뢰베 파울
Original Assignee
하인리히 벡커, 베른하르트 벡크
훽스트 아크티엔게젤샤프트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 하인리히 벡커, 베른하르트 벡크, 훽스트 아크티엔게젤샤프트 filed Critical 하인리히 벡커, 베른하르트 벡크
Publication of KR890000667A publication Critical patent/KR890000667A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음

Description

외래성 DNA를 메틸 영양 요구성 세균에서 발현시키기 위한 프로모터, 이의 제조방법 및 이의 용도
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플라즈미드pSE-1Prim의 제조과정을 나타낸 도이다.

Claims (7)

  1. 유전자를 이. 콜라이(E. coli) 및 메틸 영양 요구성 미생물 내에서 발현시키기 위한, 메틸로모나스 클라라(Methylomonas clara)의 천연 플라즈미드로부터 수득되는 프로모터 영역.
  2. 제1항에 있어서, 특징적 구조로 a) 프로모터내의 -35 영역에 존재하는 뉴클레오타이드 서열 TTGGTT 또는 TTGCGT 또는 TTGGCT 또는 TTGTTC, b) -10 영역에 존재하는 뉴클레오타이드 서열 TATGTT 또는 TAAAAT 또는 TATGAT, 및 c) -35 영역과 -10 영역사이에 존재하는 염기쌍 15 내지 19개의 스페이서(spacer) 그룹 중의 하나 이상을 지니는 프로모터 영역.
  3. 제1항 또는 2항에 있어서, 메틸로모나스 클라라 DSM2397의 플라즈미드 pBE3으로부터 수득되는 프로모터 영역.
  4. 제1항 내지 3항중의 어느 한 항에 있어서, 표 1에 기재된 서열중 하나를 지니는 프로모터 벡터.
  5. 제1항 내지 4항중 어느 한 항에 청구된 프로모터 영역을 함유하는 하이브리드 벡터.
  6. 제5항에 청구된 하이브리드 벡터를 함유하는 이. 클라이 또는 메틸 영양 요구성 미생물.
  7. 제6항에 청구된 이. 콜라이 또는 메틸 영양 요구성 미생물에 의해 발현된 폴리펩타이드.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880007521A 1987-06-24 1988-06-22 외래성 dna를 메틸 영양 요구성 세균에서 발현시키기 위한 프로모터, 이의 제조방법 및 이의 용도 KR890000667A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3720834.9 1987-06-24
DE19873720834 DE3720834A1 (de) 1987-06-24 1987-06-24 Promotoren zur expression von fremd-dna in methylotrophen bakterien, deren gewinnung und deren verwendung

Publications (1)

Publication Number Publication Date
KR890000667A true KR890000667A (ko) 1989-03-16

Family

ID=6330183

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019880007521A KR890000667A (ko) 1987-06-24 1988-06-22 외래성 dna를 메틸 영양 요구성 세균에서 발현시키기 위한 프로모터, 이의 제조방법 및 이의 용도

Country Status (13)

Country Link
EP (1) EP0296484A3 (ko)
JP (1) JPS6430588A (ko)
KR (1) KR890000667A (ko)
CN (1) CN1030939A (ko)
AU (1) AU1828388A (ko)
DE (1) DE3720834A1 (ko)
DK (1) DK345688A (ko)
FI (1) FI883005A (ko)
IL (1) IL86849A0 (ko)
NO (1) NO882792L (ko)
NZ (1) NZ225121A (ko)
PT (1) PT87809B (ko)
ZA (1) ZA884486B (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102123512B1 (ko) * 2019-03-12 2020-06-16 한국에너지기술연구원 메타노트로프(Methanotroph)에서 유래한 신규 메타노트로프-대장균 셔틀 벡터

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX307031B (es) 2008-11-14 2012-12-13 Fujian Fuda Biotech Co Ltd Una fitasa escherichia coli no-k12 termotolerante y su produccion.
KR20150036502A (ko) 2012-07-13 2015-04-07 칼리스타, 인코포레이티드 바이오리파이너리 시스템, 이의 방법 및 조성물
WO2014047209A1 (en) 2012-09-18 2014-03-27 Calysta Energy, Inc. Propylene synthesis using engineered enzymes
US10480016B2 (en) 2012-10-15 2019-11-19 Calysta, Inc. Genetically engineered microorganisms for biological oxidation of hydrocarbons
US9845474B2 (en) 2012-10-24 2017-12-19 Calysta, Inc. Engineering of multi-carbon substrate utilization pathways in methanotrophic bacteria
MY173663A (en) 2012-11-09 2020-02-14 Calysta Inc Compositions and methods for biological production of fatty acid derivatives
CN105378077A (zh) 2013-06-18 2016-03-02 凯利斯塔公司 用于使用乳酸脱氢酶转化体从c1化合物以生物方式产生乳酸的组合物和方法
EP3097185A4 (en) 2014-01-16 2017-08-09 Calysta, Inc. Carbohydrate-enriched recombinant microorganisms
EP3094718A4 (en) 2014-01-16 2017-06-07 Calysta, Inc. Microorganisms for the enhanced production of amino acids and related methods
WO2015175809A1 (en) 2014-05-15 2015-11-19 Calysta, Inc. Methods for biological production of very long carbon chain compounds
WO2020139974A1 (en) 2018-12-27 2020-07-02 Calysta, Inc. Glycogen-null methanotrophs and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0098967B1 (de) * 1982-06-11 1989-08-09 Hoechst Aktiengesellschaft Verfahren zur Herstellung von obligat methylotrophen, Fremd-DNA exprimierenden Bakterien und dafür geeignete Plasmide und Wirtsorganismen
DE3635583A1 (de) * 1986-10-20 1988-04-21 Hoechst Ag Hybridplasmid zur replikation in methylotrophen mikroorganismen und verfahren zu dessen herstellung

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102123512B1 (ko) * 2019-03-12 2020-06-16 한국에너지기술연구원 메타노트로프(Methanotroph)에서 유래한 신규 메타노트로프-대장균 셔틀 벡터

Also Published As

Publication number Publication date
NZ225121A (en) 1990-08-28
AU1828388A (en) 1989-01-05
EP0296484A2 (de) 1988-12-28
FI883005A (fi) 1988-12-25
EP0296484A3 (de) 1990-01-17
PT87809B (pt) 1992-10-30
NO882792L (no) 1988-12-27
FI883005A0 (fi) 1988-06-22
NO882792D0 (no) 1988-06-23
PT87809A (pt) 1989-05-31
IL86849A0 (en) 1988-11-30
DK345688D0 (da) 1988-06-23
CN1030939A (zh) 1989-02-08
DE3720834A1 (de) 1989-01-05
DK345688A (da) 1988-12-25
ZA884486B (en) 1990-04-25
JPS6430588A (en) 1989-02-01

Similar Documents

Publication Publication Date Title
KR890000667A (ko) 외래성 dna를 메틸 영양 요구성 세균에서 발현시키기 위한 프로모터, 이의 제조방법 및 이의 용도
DE69027526T2 (de) Herstellung von proteinen mittels homologer rekombination
IE841726L (en) Molecular cloning and expression in industrial microorganism¹species
DE69013520T2 (de) Stabile integration von dna in bakterielle genome.
JPS5616561A (en) Two-part adhesive
NO171983C (no) Fremstilling av polypeptider med human-gammainterferon-aktivitet og dna-sekvens derav
KR890700675A (ko) 새로운 단백질 분해 효소
AU586874B2 (en) Invasive microorganisms
ES2061719T3 (es) Proceso para la produccion de proteinas.
KR910001046A (ko) 비융합 단백질을 제조하기 위한 미생물내 발현용 발현벡터
KR850004275A (ko) 프로모터 및 그의 이용
ATE72837T1 (de) Vielfach amplifizierbare vektoren fuer hohe expression exogener dns.
JPS6439988A (en) Improved promoter, vector containing same, plasmid containing said vector and host cell containing said plasmid
AU4444589A (en) Expression enhancer and use thereof to increase the yield in the expression of recombinant genes
ATE66690T1 (de) Unikale, monogenetische streptokinase, ihre herstellung und verwendung.
KR880012770A (ko) Dna 하이브리드 반응용 프로브의 제조방법
JPS6467181A (en) Microorganism containing plasmid produced by gene recombination and production of lichenase with said microorganism
JPS6434290A (en) Hybrid promoter
JPS6471492A (en) Synthetic gene of cystatin b, corresponding recombinant plasmid and recombinant
KR950005990A (ko) 인간 인터류킨-4(human interleukin-4)의 효모에서의 발현
KR860003341A (ko) 유전자 조작된 새로운 군주에 의한 페닐알라닌 제조방법
JPS6416586A (en) Production of baniii restriction enzyme
PT86744A (pt) Vectores de expressao retroviral e metodos para produzir antigenios de vhb
KR870007278A (ko) 유전자 조환에 의한 사람알파 인터페론의 제조 방법
JPS6427476A (en) Gene and use thereof

Legal Events

Date Code Title Description
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid