KR890700675A - 새로운 단백질 분해 효소 - Google Patents

새로운 단백질 분해 효소

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Publication number
KR890700675A
KR890700675A KR1019880701597A KR880701597A KR890700675A KR 890700675 A KR890700675 A KR 890700675A KR 1019880701597 A KR1019880701597 A KR 1019880701597A KR 880701597 A KR880701597 A KR 880701597A KR 890700675 A KR890700675 A KR 890700675A
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KR
South Korea
Prior art keywords
serine protease
binding sequence
dna binding
isolated
amino acid
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KR1019880701597A
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English (en)
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KR970009949B1 (en
Inventor
비.세이멀 바브루
스태빈스키 이트쟈크
Original Assignee
로버트 디.웨이스트
암젠 인코포레이티드
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Application filed by 로버트 디.웨이스트, 암젠 인코포레이티드 filed Critical 로버트 디.웨이스트
Publication of KR890700675A publication Critical patent/KR890700675A/ko
Application granted granted Critical
Publication of KR970009949B1 publication Critical patent/KR970009949B1/ko

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/02—Acyclic radicals, not substituted by cyclic structures
    • C07H15/12—Acyclic radicals, not substituted by cyclic structures attached to a nitrogen atom of the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Cosmetics (AREA)

Abstract

내용 없음.

Description

새로운 단백질 분해 효소
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 프로테아제 TW 7에 대한 게노믹 클로운의 뉴클레오티드 결합서열을 나타낸 것이고;

Claims (23)

  1. ATCC 제 22563호로 기탁한 트리티라치움 앨범 림버 균주 배양액으로부터 분리하여 정제한 세린 프로테아제.
  2. 제1항에 있어서, 제2도에 나타난 바와같은 아미노산 결합서열을 가지는 세린 프로테아제.
  3. 제l항에 있어서, 제8도에 나타난 바와같은 아미노산 결합서열을 가지는 세린 프로테아제.
  4. ATCC 제 22563호 트리티라치움 앨범 림버 균주 배양액으로부터 분리한 세린 프로테아제를 암호화하는 유전자와 혼성화할수 있는 올리고뉴클레오티드 프로브.
  5. 제4항에 있어서, 다음 뉴클레오티드 결합서열을 가지는 올리고뉴클레오티드 프로브:
    5', GCT GCT AIT CCG GCA ACG TGA GGA GTC GCC ATG GAC GTT CC 3'
  6. ATCC 제1 22563호 트리티라치움 앨범 림버 균주 배양액으로부터 분리한 세린 프로테아제의 구조 형태를 가지는 세린 프로테아제를 원시핵 또는 성숙핵 숙주세포에서 완전히 발현시키는데 사용되는 DNA 결합서열.
  7. 제6항에 있어서, 제1도에 나타난 바와같은 뉴클레오티드 결합서열을 가지는 DNA 결합서열.
  8. 제7항에 있어서, 제 7도에 나타난 바와같은 뉴클레오티드 결합서열을 가지는 DNA 결합서열.
  9. 트리티라치움 앨범 균주 배양액으로부터 분리한 세린 프로테아제를 세정제 조성물내에 효과적인 량으로 포함하는 조성물
  10. 제9항에 있어서, 상기 세린 프로테아제는 제2도에 나타낸 아미노산 결합서열을 가짐을 특징으로 하는 조성물.
  11. 제9항에 있어서, 상기 세린 프로테아제는 제8도에 나타난 아미노산 결합서열을 가짐을 특징으로 하는 조성물.
  12. 변형된 세포 배양액내에서 제6항에 따른 DNA 결합서열을 발현시킬 수 있는 발현 백터.
  13. 제12항에 따른 발현 벡터로 변형된 세포 배양액.
  14. 제13항에 따라 대장균 균주를 변형시킴으로써 얻어지는 미생물.
  15. 제2도에 나타난 바와같은 아미노산 결합서열을 가지는 세린 프로테아제를 생산할 수 있는 대장균 균주.
  16. 제8도에 나타난 바와같은 아미노산 결합서열을 가지는 세린 프로테아제를 생산할 수 있는 대장균 균주.
  17. ATCC 제22563호 트리티라치움 앨범 림버 균주 배양액으로부터 분리한 세린 프로테아제의 구조 형태를 가지며 외인성 DNA 결합서열의 원시핵 또는 성숙핵 세포내 발현 생성물임을 특징으로 하는 분리하여 정제한 세린 프로테아제.
  18. 제17항에 있어서, 외인성 DNA는 cDNA 결합서열임을 특징으로 하는 세린 프로테아제.
  19. 제17항에 있어서, 외인성 DNA 결합서열은 게노믹 DNA 결합서열임을 특징으로 하는 세린 프로테아제.
  20. 제17항에 있어서, 외인성 DNA 결합서열은 제조된 DNA 결합서열임을 특징으로 하는 세린 프로테아제.
  21. 제17항에 있어서, 제2도에 나타난 바와같은 구조 형태를 가지는 세린 프로테아제.
  22. 제17항에 있어서, 제8도에 나타난 바와 같은 구조 형태를 가지는 세린 프로테아제.
  23. ATCC 제 22563호 트리티라치움 앨범 림버 군주 배양액으로부터 분리한 세린 프로테아제의 구조 형태를 가지는 세린 프로테아제의 제조방법에 있어서, 원시핵 또는 성숙핵 숙주세포내에서 바람직한 세린 프로테아제를 완전히 발현시키는데 유용한 DNA 결합서열을 포함하는 DNA 벡터로 변형되거나 전이된 원시핵 또는 성숙핵 숙주세포를 적합한 배양 조건하에 배양시킨후, 상기 벡터내의 DNA 결합서열을 발현시킨 바람직한 세린 프로테아제를 분리시키는 과정으로 구성되는 세린 프로테아제 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR88701597A 1987-04-03 1988-12-03 Novel serine proteolytic enzyme and its process KR970009949B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3581687A 1987-04-03 1987-04-03
US035816 1987-04-03
PCT/US1988/001040 WO1988007581A1 (en) 1987-04-03 1988-03-28 Novel proteolytic enzymes

Publications (2)

Publication Number Publication Date
KR890700675A true KR890700675A (ko) 1989-04-26
KR970009949B1 KR970009949B1 (en) 1997-06-19

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KR88701597A KR970009949B1 (en) 1987-04-03 1988-12-03 Novel serine proteolytic enzyme and its process

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Country Link
EP (1) EP0309550B1 (ko)
JP (1) JP2731407B2 (ko)
KR (1) KR970009949B1 (ko)
AT (1) ATE139567T1 (ko)
AU (1) AU617996B2 (ko)
CA (1) CA1331154C (ko)
DE (1) DE3855375T2 (ko)
DK (1) DK672088A (ko)
FI (1) FI100602B (ko)
IL (1) IL85954A (ko)
NO (1) NO179217C (ko)
WO (1) WO1988007581A1 (ko)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8726999D0 (en) * 1987-11-18 1987-12-23 Unilever Plc Enzymatic liquid detergent composition
US5288627A (en) * 1988-01-07 1994-02-22 Novo Nordisk A/S Endoprotease from Fusarium oxysporumDSM 2672 for use in detergents
CA2069147A1 (en) * 1991-05-22 1992-11-23 Kazuaki Kitano Dna and its use
EP0623672A1 (en) * 1993-05-04 1994-11-09 Research Institute For Plant Protection New alkaline serine protease of Paecilomyces lilacinus
DE10001140C2 (de) * 2000-01-13 2003-06-12 Gsf Forschungszentrum Umwelt Oligonukleotide zur Amplifikation und zum Nachweis von bakteriellen Genen für extrazelluläre alkalische Metallopeptidasen (Apr), neutrale Metallopeptidasen (Npr) und Serinpeptidasen (Spr)
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE10105911A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
JP4880469B2 (ja) * 2003-10-23 2012-02-22 ノボザイムス アクティーゼルスカブ 洗剤中で改良された安定性を有するプロテアーゼ
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
FI121712B (fi) 2009-04-30 2011-03-15 Ab Enzymes Oy Uusi sieniperäinen proteaasi ja sen käyttö
FI121711B (fi) 2009-04-30 2011-03-15 Ab Enzymes Oy Sieniperäinen seriiniproteaasi ja sen käyttö
FI121851B (fi) 2009-07-08 2011-05-13 Ab Enzymes Oy Sieniperäinen proteaasi ja sen käyttö
FI123942B (fi) 2010-10-29 2013-12-31 Ab Enzymes Oy Sieniperäisen seriiniproteaasin variantteja
FI123425B (fi) 2011-03-31 2013-04-30 Ab Enzymes Oy Proteaasientsyymi ja sen käytöt
CN108603187A (zh) 2015-11-24 2018-09-28 诺维信公司 具有蛋白酶活性的多肽以及编码它们的多核苷酸
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1240058A (en) * 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
US3623957A (en) * 1970-01-21 1971-11-30 Baxter Laboratories Inc Preparation of microbial alkaline protease by fermentation with bacillus subtilis, variety licheniformis
WO1988003448A1 (fr) * 1986-11-14 1988-05-19 Erich Goergens Procede et dispositif de transmission et d'entretien d'energie de deformation
PT89702B (pt) * 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem

Also Published As

Publication number Publication date
EP0309550A1 (en) 1989-04-05
FI100602B (fi) 1998-01-15
IL85954A (en) 1994-11-28
FI885635A0 (fi) 1988-12-02
EP0309550A4 (en) 1990-01-08
AU1571688A (en) 1988-11-02
FI885635A (fi) 1988-12-02
JPH01502798A (ja) 1989-09-28
NO179217C (no) 1996-08-28
ATE139567T1 (de) 1996-07-15
CA1331154C (en) 1994-08-02
AU617996B2 (en) 1991-12-12
DK672088A (da) 1989-02-03
WO1988007581A1 (en) 1988-10-06
EP0309550B1 (en) 1996-06-19
KR970009949B1 (en) 1997-06-19
DE3855375T2 (de) 1996-11-14
DK672088D0 (da) 1988-12-02
NO179217B (no) 1996-05-20
NO885391L (no) 1989-02-03
JP2731407B2 (ja) 1998-03-25
IL85954A0 (en) 1988-09-30
DE3855375D1 (de) 1996-07-25
NO885391D0 (no) 1988-12-02

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