ES8607388A1 - Un metodo para preparar que tiene sustancialmente la misma secuencia de aminoacidos que la superoxido-dismutasa humana. - Google Patents

Un metodo para preparar que tiene sustancialmente la misma secuencia de aminoacidos que la superoxido-dismutasa humana.

Info

Publication number
ES8607388A1
ES8607388A1 ES536441A ES536441A ES8607388A1 ES 8607388 A1 ES8607388 A1 ES 8607388A1 ES 536441 A ES536441 A ES 536441A ES 536441 A ES536441 A ES 536441A ES 8607388 A1 ES8607388 A1 ES 8607388A1
Authority
ES
Spain
Prior art keywords
superoxide dismutase
expression
cloning
microorganisms
deposited
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES536441A
Other languages
English (en)
Other versions
ES536441A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Publication of ES8607388A1 publication Critical patent/ES8607388A1/es
Publication of ES536441A0 publication Critical patent/ES536441A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

METODO PARA PREPARAR UN POLIPEPTIDO QUE TIENE SUSTANCIALMENTE LA MISMA SECUENCIA DE AMINOACIDOS QUE LA SUPEROXIDO-DISMUTASA HUMANA. CONSISTE EN HACER CRECER UN MICROORGANISMO UNICELULAR QUE CONTIENE UNA CONSTRUCCION DE ADN FUNCIONAL EN EL MICROORGANISMO Y QUE TIENE UN GEN ESTRUCTURAL DE LA SUPEROXIDO-DISMUTASA HUMANA CAPAZ DE EXPRESION EN DICHO ORGANISMO, CON LO CUAL, EL GEN SE EXPRESA Y SE PRODUCE LA SUPEROXIDO-DISMUTASA HUMANA; Y AISLAR DICHA SUPEROXIDO-DISMUTASA HUMANA. LA CONSTRUCCION DE ADN COMPRENDE EN ORDEN AGUAS ABAJO DE LA TRANSCRIPCION: UN PROMOTOR; UN SITIO DE FIJACION DE RIBOSOMA; UN CODON DE INICIACION; UN GEN ESTRUCTURAL DE LA SUPEROXIDO-DISMUTASA HUMANA EN UN MARCO DE LECTURA CON EL CODON DE INICIACION QUE TIENE UN CODON DE DETENCION EN UN EXTREMO TERMINAL; Y UN TERMINADOR DE LA TRANSCRIPCION.
ES536441A 1983-10-03 1984-10-02 Un metodo para preparar que tiene sustancialmente la misma secuencia de aminoacidos que la superoxido-dismutasa humana. Expired ES8607388A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53860783A 1983-10-03 1983-10-03
US60941284A 1984-05-11 1984-05-11

Publications (2)

Publication Number Publication Date
ES8607388A1 true ES8607388A1 (es) 1986-05-16
ES536441A0 ES536441A0 (es) 1986-05-16

Family

ID=27065872

Family Applications (1)

Application Number Title Priority Date Filing Date
ES536441A Expired ES8607388A1 (es) 1983-10-03 1984-10-02 Un metodo para preparar que tiene sustancialmente la misma secuencia de aminoacidos que la superoxido-dismutasa humana.

Country Status (13)

Country Link
EP (2) EP0656418B1 (es)
JP (4) JP2609446B2 (es)
AT (1) ATE155527T1 (es)
CA (1) CA1296272C (es)
DE (3) DE3486452T2 (es)
DK (1) DK243785A (es)
ES (1) ES8607388A1 (es)
GR (1) GR80523B (es)
HU (1) HU207533B (es)
IE (1) IE59518B1 (es)
IL (1) IL73156A (es)
PT (1) PT79308B (es)
WO (1) WO1985001503A1 (es)

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Publication number Priority date Publication date Assignee Title
US5196335A (en) * 1983-04-29 1993-03-23 Yeda Research And Development Co., Ltd. Human superoxide dismutase cDNA
US5714362A (en) * 1983-04-29 1998-02-03 Yeda Research And Development Co. Ltd. Human superoxide dismutase cDNA
AU582536B2 (en) * 1983-04-29 1989-04-06 Yeda Research And Development Co. Ltd. Human superoxide dismutase cdna
US5710033A (en) * 1983-10-03 1998-01-20 Chiron Corporation Superoxide dismutase cloning and expression in microorganisms
US5691139A (en) * 1983-10-03 1997-11-25 Chiron Corporation Genetic modification of superoxide dismutase to increase expression in microorganisms
ATE102250T1 (de) * 1984-05-11 1994-03-15 Chiron Corp Erhoehte hefetranskription unter verwendung einer hybridkonstruktion der promotorregion.
US5759816A (en) * 1984-08-27 1998-06-02 Bio-Technology General Corp. Expression vectors containing λPL promoter and T1 T2 rRNA termination sequence plasmids containing the vectors hosts containing the plasmids and related methods
CA1340597C (en) * 1984-08-27 1999-06-22 Haim Aviv Expression vectors containing pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, plasmids containing the vectors, hosts containing the plasmids and related methods
US5162217A (en) * 1984-08-27 1992-11-10 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda PL promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
US5112744A (en) * 1984-08-27 1992-05-12 Bio-Technology General Corp. Plasmids for the production of human Cu-Zn superoxide dismutase, bacterial hosts containing the plasmids and methods for production and recovery of such superoxide dismutase
US5143836A (en) * 1984-08-27 1992-09-01 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda pl promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
JPS61111690A (ja) * 1984-11-06 1986-05-29 Ube Ind Ltd 組換えｄｎａおよびその用途
US5342921A (en) * 1985-03-28 1994-08-30 Chiron Corporation Superoxide dismutase fusion polypeptides for expression of mammalian proteins
EP0225887B1 (en) * 1985-04-08 1994-06-08 Amgen Inc. A method and a hybrid promoter for controlling exogenous gene transcription
US5248603A (en) * 1985-09-03 1993-09-28 Symbicom Aktiebolag Superoxide dismutase
DK402785D0 (da) * 1985-09-03 1985-09-03 Syn Tek Ab Fremgangsmaade til fremstilling af et enzym
EP0213628A3 (en) * 1985-09-03 1988-09-21 Yeda Research And Development Company, Ltd. Expression of superoxide dismutase in eukaryotic cells
US5270195A (en) * 1985-11-22 1993-12-14 Bio-Technology General Corp. Plasmids for expression and method of producing a human manganese superoxide dimutase analog
IE59498B1 (en) * 1985-11-22 1994-03-09 Bio Technology General Corp Human manganese superoxide dismutase analog, plasmid for its expression and method of recovering it in enzymatically active form
US6610520B1 (en) 1985-11-22 2003-08-26 Bio-Technology General Corp. Gene encoding human manganese superoxide dismutase and recombinant polypeptide encoded thereby
JPS62130689A (ja) * 1985-12-04 1987-06-12 Nippon Kayaku Co Ltd ランナウエイ型プラスミド及びそれを利用したヒトｓｏｄの製造方法
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
CA1339299C (en) * 1986-09-12 1997-08-19 Jacob R. Hartman Human manganese superoxide dismutase dna; its expression; method of recovering human manganese superoxide dismutase, human manganese superoxide dismutase analogs or human manganese superoxide dismutase mutants; uses;compositions; and methods of treatment
US5260204A (en) * 1987-03-14 1993-11-09 Boehringer Ingelheim International Gmbh Human manganese superoxide dismutase (hMn-SOD)
ATE132902T1 (de) * 1987-03-14 1996-01-15 Boehringer Ingelheim Int Humane mangan-superoxiddismutase (hmn-sod)
DE3868333D1 (de) * 1987-03-16 1992-03-26 Chiron Corp Superoxiddismutase-polymere.
US5171680A (en) * 1988-06-14 1992-12-15 Chiron Corporation Superoxide dismutase analogs having novel binding properties
JP2942578B2 (ja) * 1988-06-14 1999-08-30 カイロン コーポレイション 新しい結合特性を有する超酸化物不均化酵素類縁体
WO1990003431A1 (en) * 1988-09-26 1990-04-05 The Salk Institute Biotechnology/Industrial Associates, Inc. Mixed feed recombinant yeast fermentation
US5656458A (en) * 1988-11-04 1997-08-12 Chiron Corporation Expression and processing of amino terminus acetylated FGF's in yeast
DD297841A5 (de) * 1989-02-13 1992-01-23 The Salk Institute Biotechnology/Industrial Associates,Us Herstellung von superoxiddismutase in pichia pastoris hefezellen
DK455789D0 (da) * 1989-09-15 1989-09-15 Symbicom Ab Polypeptid
US5202317A (en) * 1990-09-13 1993-04-13 The Regents Of The University Of California Synthetic drug molecules that mimic metalloenzymes
DE4038563A1 (de) * 1990-12-04 1992-06-11 Gruenenthal Gmbh Verwendung von superoxiddismutasen zur prophylaxe und/oder behandlung von organversagen bei risikopatienten mit polytrauma
JPH0767393B2 (ja) * 1991-03-13 1995-07-26 萩原 義秀 ポリペプチドの発現方法
CN1055502C (zh) * 1992-04-10 2000-08-16 萩原义秀 表达多肽的方法
DE4239877C1 (de) * 1992-11-27 1994-03-17 Boehringer Ingelheim Int Stabilisierte Superoxid-Dismutase (SOD)-Zusammensetzung
CA2215317C (en) 1995-03-24 2002-07-23 Focal, Inc. Reduction of adhesions using controlled delivery of active oxygen inhibitors
BE1009650A5 (fr) * 1995-10-12 1997-06-03 Genencor Int Systeme d'expression, vecteur et cellule transformee par ce vecteur.
KR20070111556A (ko) * 2005-10-24 2007-11-22 (주)케어젠 섬유아세포 성장인자의 기능을 가지는 펩티드 및 이를이용한 화장품
WO2007105024A1 (en) * 2006-03-15 2007-09-20 Isocell Pharma S.A. Pharmaceutical compositions comprising sods and prolamine based peptide fragments
CN102453725B (zh) * 2010-11-02 2014-06-11 杭州纽龙生物科技有限公司 一种重组载体、包含该重组载体的重组菌株及制备方法

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US4042689A (en) * 1975-09-09 1977-08-16 Diagnostic Data, Inc. Alkylated orgotein
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FR2441659A1 (fr) * 1978-11-14 1980-06-13 Anvar Nouveaux plasmides hybrides et microorganismes les contenant
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JPS57155991A (en) * 1981-03-23 1982-09-27 Green Cross Corp:The Preparation of superoxide dismutase derived from human placenta
JPS6012025B2 (ja) * 1981-07-06 1985-03-29 わかもと製薬株式会社 ス−パ−オキサイドジスムタ−ゼの精製法

Also Published As

Publication number Publication date
JP2609446B2 (ja) 1997-05-14
JPH06165674A (ja) 1994-06-14
DE3486452T2 (de) 1997-11-06
IE59518B1 (en) 1994-03-09
DE3486452D1 (de) 1997-08-21
IL73156A (en) 1991-08-16
EP0656418A1 (en) 1995-06-07
DE3486493D1 (de) 2003-01-23
CA1296272C (en) 1992-02-25
GR80523B (en) 1985-01-30
HU207533B (en) 1993-04-28
EP0138111A1 (en) 1985-04-24
DE3486493T2 (de) 2003-05-22
IE842506L (en) 1985-04-03
PT79308B (en) 1986-09-08
DK243785D0 (da) 1985-05-31
ES536441A0 (es) 1986-05-16
JP2635500B2 (ja) 1997-07-30
DK243785A (da) 1985-05-31
WO1985001503A1 (en) 1985-04-11
EP0656418B1 (en) 2002-12-04
JPH07298884A (ja) 1995-11-14
JP2664123B2 (ja) 1997-10-15
JPH05192140A (ja) 1993-08-03
PT79308A (en) 1984-11-01
EP0138111B1 (en) 1991-11-21
DE3485284D1 (de) 1992-01-02
JPS60137286A (ja) 1985-07-20
IL73156A0 (en) 1985-01-31
HUT37458A (en) 1985-12-28
ATE155527T1 (de) 1997-08-15
JP2568794B2 (ja) 1997-01-08

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20050709