DE3486493D1 - Klonieren von Superoxiddismutase und Expression in Mikroorganismen - Google Patents

Klonieren von Superoxiddismutase und Expression in Mikroorganismen

Info

Publication number
DE3486493D1
DE3486493D1 DE3486493T DE3486493T DE3486493D1 DE 3486493 D1 DE3486493 D1 DE 3486493D1 DE 3486493 T DE3486493 T DE 3486493T DE 3486493 T DE3486493 T DE 3486493T DE 3486493 D1 DE3486493 D1 DE 3486493D1
Authority
DE
Germany
Prior art keywords
superoxide dismutase
human cytoplasmic
expression
acetylated
dna construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3486493T
Other languages
English (en)
Other versions
DE3486493T2 (de
Inventor
Robert A Hallewell
Guy T Mullenbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of DE3486493D1 publication Critical patent/DE3486493D1/de
Publication of DE3486493T2 publication Critical patent/DE3486493T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
DE3486493T 1983-10-03 1984-09-25 Klonieren von Superoxiddismutase und Expression in Mikroorganismen Expired - Lifetime DE3486493T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53860783A 1983-10-03 1983-10-03
US60941284A 1984-05-11 1984-05-11

Publications (2)

Publication Number Publication Date
DE3486493D1 true DE3486493D1 (de) 2003-01-23
DE3486493T2 DE3486493T2 (de) 2003-05-22

Family

ID=27065872

Family Applications (3)

Application Number Title Priority Date Filing Date
DE8484111416T Expired - Lifetime DE3485284D1 (de) 1983-10-03 1984-09-25 Klonieren von superoxid-dismutase und expression in mikroorganismen.
DE3486452T Expired - Lifetime DE3486452T2 (de) 1983-10-03 1984-09-25 Superoxiddismutase und deren Expression in Mikroorganismen
DE3486493T Expired - Lifetime DE3486493T2 (de) 1983-10-03 1984-09-25 Klonieren von Superoxiddismutase und Expression in Mikroorganismen

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE8484111416T Expired - Lifetime DE3485284D1 (de) 1983-10-03 1984-09-25 Klonieren von superoxid-dismutase und expression in mikroorganismen.
DE3486452T Expired - Lifetime DE3486452T2 (de) 1983-10-03 1984-09-25 Superoxiddismutase und deren Expression in Mikroorganismen

Country Status (13)

Country Link
EP (2) EP0656418B1 (de)
JP (4) JP2609446B2 (de)
AT (1) ATE155527T1 (de)
CA (1) CA1296272C (de)
DE (3) DE3485284D1 (de)
DK (1) DK243785A (de)
ES (1) ES8607388A1 (de)
GR (1) GR80523B (de)
HU (1) HU207533B (de)
IE (1) IE59518B1 (de)
IL (1) IL73156A (de)
PT (1) PT79308B (de)
WO (1) WO1985001503A1 (de)

Families Citing this family (43)

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Publication number Priority date Publication date Assignee Title
US5196335A (en) * 1983-04-29 1993-03-23 Yeda Research And Development Co., Ltd. Human superoxide dismutase cDNA
AU582536B2 (en) * 1983-04-29 1989-04-06 Yeda Research And Development Co. Ltd. Human superoxide dismutase cdna
US5714362A (en) * 1983-04-29 1998-02-03 Yeda Research And Development Co. Ltd. Human superoxide dismutase cDNA
US5691139A (en) * 1983-10-03 1997-11-25 Chiron Corporation Genetic modification of superoxide dismutase to increase expression in microorganisms
US5710033A (en) * 1983-10-03 1998-01-20 Chiron Corporation Superoxide dismutase cloning and expression in microorganisms
ATE102250T1 (de) * 1984-05-11 1994-03-15 Chiron Corp Erhoehte hefetranskription unter verwendung einer hybridkonstruktion der promotorregion.
US5162217A (en) * 1984-08-27 1992-11-10 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda PL promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
CA1340597C (en) * 1984-08-27 1999-06-22 Haim Aviv Expression vectors containing pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, plasmids containing the vectors, hosts containing the plasmids and related methods
US5112744A (en) * 1984-08-27 1992-05-12 Bio-Technology General Corp. Plasmids for the production of human Cu-Zn superoxide dismutase, bacterial hosts containing the plasmids and methods for production and recovery of such superoxide dismutase
US5143836A (en) * 1984-08-27 1992-09-01 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda pl promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
US5759816A (en) * 1984-08-27 1998-06-02 Bio-Technology General Corp. Expression vectors containing λPL promoter and T1 T2 rRNA termination sequence plasmids containing the vectors hosts containing the plasmids and related methods
JPS61111690A (ja) * 1984-11-06 1986-05-29 Ube Ind Ltd 組換えｄｎａおよびその用途
US5342921A (en) * 1985-03-28 1994-08-30 Chiron Corporation Superoxide dismutase fusion polypeptides for expression of mammalian proteins
JP2615027B2 (ja) * 1985-04-08 1997-05-28 アムジェン インコーポレイテッド 外来遺伝子の転写を制御するための方法及びハイブリッドプロモーター
EP0213628A3 (de) * 1985-09-03 1988-09-21 Yeda Research And Development Company, Ltd. Expression von Superoxiddismutase in eukaryotischen Zellen
US5248603A (en) * 1985-09-03 1993-09-28 Symbicom Aktiebolag Superoxide dismutase
DK402785D0 (da) * 1985-09-03 1985-09-03 Syn Tek Ab Fremgangsmaade til fremstilling af et enzym
IE59498B1 (en) * 1985-11-22 1994-03-09 Bio Technology General Corp Human manganese superoxide dismutase analog, plasmid for its expression and method of recovering it in enzymatically active form
US6610520B1 (en) * 1985-11-22 2003-08-26 Bio-Technology General Corp. Gene encoding human manganese superoxide dismutase and recombinant polypeptide encoded thereby
US5270195A (en) * 1985-11-22 1993-12-14 Bio-Technology General Corp. Plasmids for expression and method of producing a human manganese superoxide dimutase analog
JPS62130689A (ja) * 1985-12-04 1987-06-12 Nippon Kayaku Co Ltd ランナウエイ型プラスミド及びそれを利用したヒトｓｏｄの製造方法
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
EP0676472A1 (de) * 1987-03-14 1995-10-11 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Humane Mangan-Superoxiddismutase (hMn-SOD)
US5260204A (en) * 1987-03-14 1993-11-09 Boehringer Ingelheim International Gmbh Human manganese superoxide dismutase (hMn-SOD)
EP0283244B1 (de) * 1987-03-16 1992-02-12 Chiron Corporation Superoxiddismutase-Polymere
EP0284105B1 (de) * 1987-03-27 1995-11-15 Bio-Technology General Corporation Menschliches Mangansuperoxiddismutase und Behandlungsverfahren
WO1989012677A1 (en) * 1988-06-14 1989-12-28 Chiron Corporation Superoxide dismutase analogs having novel binding properties
US5171680A (en) * 1988-06-14 1992-12-15 Chiron Corporation Superoxide dismutase analogs having novel binding properties
AU4332589A (en) * 1988-09-26 1990-04-18 Salk Institute Biotechnology/Industrial Associates, Inc., The Mixed feed recombinant yeast fermentation
US5656458A (en) * 1988-11-04 1997-08-12 Chiron Corporation Expression and processing of amino terminus acetylated FGF's in yeast
AU5196290A (en) * 1989-02-13 1990-09-05 Salk Institute Biotechnology/Industrial Associates, Inc., The Production of superoxide dismutase in pichia pastoris yeast cells
DK455789D0 (da) * 1989-09-15 1989-09-15 Symbicom Ab Polypeptid
US5202317A (en) * 1990-09-13 1993-04-13 The Regents Of The University Of California Synthetic drug molecules that mimic metalloenzymes
DE4038563A1 (de) * 1990-12-04 1992-06-11 Gruenenthal Gmbh Verwendung von superoxiddismutasen zur prophylaxe und/oder behandlung von organversagen bei risikopatienten mit polytrauma
JPH0767393B2 (ja) * 1991-03-13 1995-07-26 萩原 義秀 ポリペプチドの発現方法
CN1055502C (zh) * 1992-04-10 2000-08-16 萩原义秀 表达多肽的方法
DE4239877C1 (de) * 1992-11-27 1994-03-17 Boehringer Ingelheim Int Stabilisierte Superoxid-Dismutase (SOD)-Zusammensetzung
CA2215317C (en) * 1995-03-24 2002-07-23 Focal, Inc. Reduction of adhesions using controlled delivery of active oxygen inhibitors
BE1009650A5 (fr) * 1995-10-12 1997-06-03 Genencor Int Systeme d'expression, vecteur et cellule transformee par ce vecteur.
KR20070111556A (ko) * 2005-10-24 2007-11-22 (주)케어젠 섬유아세포 성장인자의 기능을 가지는 펩티드 및 이를이용한 화장품
WO2007105024A1 (en) * 2006-03-15 2007-09-20 Isocell Pharma S.A. Pharmaceutical compositions comprising sods and prolamine based peptide fragments
CN102453725B (zh) * 2010-11-02 2014-06-11 杭州纽龙生物科技有限公司 一种重组载体、包含该重组载体的重组菌株及制备方法

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US4070459A (en) * 1975-12-09 1978-01-24 Diagnostic Data, Inc. N-carbamylated orgotein
US4042689A (en) * 1975-09-09 1977-08-16 Diagnostic Data, Inc. Alkylated orgotein
FR2441659A1 (fr) * 1978-11-14 1980-06-13 Anvar Nouveaux plasmides hybrides et microorganismes les contenant
US4332892A (en) * 1979-01-15 1982-06-01 President And Fellows Of Harvard College Protein synthesis
JPS5712993A (en) * 1980-06-23 1982-01-22 Fujirebio Inc Isolation of superoxide dismutase
JPS57155991A (en) * 1981-03-23 1982-09-27 Green Cross Corp:The Preparation of superoxide dismutase derived from human placenta
JPS6012025B2 (ja) * 1981-07-06 1985-03-29 わかもと製薬株式会社 ス−パ−オキサイドジスムタ−ゼの精製法

Also Published As

Publication number Publication date
DE3486493T2 (de) 2003-05-22
ES536441A0 (es) 1986-05-16
PT79308A (en) 1984-11-01
ES8607388A1 (es) 1986-05-16
JPH06165674A (ja) 1994-06-14
DK243785D0 (da) 1985-05-31
JP2568794B2 (ja) 1997-01-08
JP2664123B2 (ja) 1997-10-15
IE842506L (en) 1985-04-03
DE3485284D1 (de) 1992-01-02
DK243785A (da) 1985-05-31
PT79308B (en) 1986-09-08
EP0656418A1 (de) 1995-06-07
EP0656418B1 (de) 2002-12-04
CA1296272C (en) 1992-02-25
ATE155527T1 (de) 1997-08-15
HUT37458A (en) 1985-12-28
JPS60137286A (ja) 1985-07-20
DE3486452D1 (de) 1997-08-21
IE59518B1 (en) 1994-03-09
JP2609446B2 (ja) 1997-05-14
WO1985001503A1 (en) 1985-04-11
HU207533B (en) 1993-04-28
IL73156A0 (en) 1985-01-31
DE3486452T2 (de) 1997-11-06
IL73156A (en) 1991-08-16
EP0138111A1 (de) 1985-04-24
JPH07298884A (ja) 1995-11-14
EP0138111B1 (de) 1991-11-21
GR80523B (en) 1985-01-30
JPH05192140A (ja) 1993-08-03
JP2635500B2 (ja) 1997-07-30

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Legal Events

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