JPS57155991A - Preparation of superoxide dismutase derived from human placenta - Google Patents

Preparation of superoxide dismutase derived from human placenta

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Publication number
JPS57155991A
JPS57155991A JP4271781A JP4271781A JPS57155991A JP S57155991 A JPS57155991 A JP S57155991A JP 4271781 A JP4271781 A JP 4271781A JP 4271781 A JP4271781 A JP 4271781A JP S57155991 A JPS57155991 A JP S57155991A
Authority
JP
Japan
Prior art keywords
ammonium sulfate
fraction
superoxide dismutase
human placenta
recovered
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP4271781A
Other languages
Japanese (ja)
Inventor
Kazuo Takechi
Takeshi Takahashi
Eiichi Hasegawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Priority to JP4271781A priority Critical patent/JPS57155991A/en
Publication of JPS57155991A publication Critical patent/JPS57155991A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To separate and to purify superoxide dismutase efficiently, by removing a precipitation fraction of saturated ammonium sulfate in a specific ratio from a water extracted solution of human placenta, subjecting a supernatant liquid of the ammonium sulfate to ammonium sulfate fractionation, etc.
CONSTITUTION: Human placenta is homogenized, extracted with water, centrifuged to remove the precipitate, and a γ-globulin fraction and a raw albumin fraction are recovered from a 70W80wt% saturated ammonium sulfate fraction, etc. as precipitates. Ammonium sulfate is added to a supernatant liquid of the recovered 70W80wt% saturated ammonium sulfate in a pH of preferably about 6W8.5 to give 85W95wt% saturated ammonium sulfate, the prepared precipitate is separated from the supernatant liquid, to recover a raw superoxide dismutase fraction as a precipitate. The recovered raw superoxide dismutase fraction is optionally subjected to a combination of ammonium sulfate fractionation, anion chromatography, gel filtration, crosslinked dextran, etc. to give highly purified superoxide dismutase.
COPYRIGHT: (C)1982,JPO&Japio
JP4271781A 1981-03-23 1981-03-23 Preparation of superoxide dismutase derived from human placenta Pending JPS57155991A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4271781A JPS57155991A (en) 1981-03-23 1981-03-23 Preparation of superoxide dismutase derived from human placenta

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4271781A JPS57155991A (en) 1981-03-23 1981-03-23 Preparation of superoxide dismutase derived from human placenta

Publications (1)

Publication Number Publication Date
JPS57155991A true JPS57155991A (en) 1982-09-27

Family

ID=12643816

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4271781A Pending JPS57155991A (en) 1981-03-23 1981-03-23 Preparation of superoxide dismutase derived from human placenta

Country Status (1)

Country Link
JP (1) JPS57155991A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985001503A1 (en) * 1983-10-03 1985-04-11 Chiron Corporation Superoxide dismutase cloning and expression in microorganisms
JPS63254181A (en) * 1987-04-09 1988-10-20 Kobayashi Kooc:Kk Antioxidant composition
US5130245A (en) * 1985-09-03 1992-07-14 Stefan Marklund Superoxide dismutase
US5248603A (en) * 1985-09-03 1993-09-28 Symbicom Aktiebolag Superoxide dismutase

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985001503A1 (en) * 1983-10-03 1985-04-11 Chiron Corporation Superoxide dismutase cloning and expression in microorganisms
US5130245A (en) * 1985-09-03 1992-07-14 Stefan Marklund Superoxide dismutase
US5248603A (en) * 1985-09-03 1993-09-28 Symbicom Aktiebolag Superoxide dismutase
US5472691A (en) * 1985-09-03 1995-12-05 Symbicom Aktiebolag Superoxide dismutase
US5788961A (en) * 1985-09-03 1998-08-04 Symbicom Aktiebolag Superoxide dismutase
JPS63254181A (en) * 1987-04-09 1988-10-20 Kobayashi Kooc:Kk Antioxidant composition
JPH0781140B2 (en) * 1987-04-09 1995-08-30 株式会社コーセー Antioxidant composition

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