JPS5774087A - Separation and purification of placental enzyme transformating angiotensin (a tase) - Google Patents

Separation and purification of placental enzyme transformating angiotensin (a tase)

Info

Publication number
JPS5774087A
JPS5774087A JP15123480A JP15123480A JPS5774087A JP S5774087 A JPS5774087 A JP S5774087A JP 15123480 A JP15123480 A JP 15123480A JP 15123480 A JP15123480 A JP 15123480A JP S5774087 A JPS5774087 A JP S5774087A
Authority
JP
Japan
Prior art keywords
tase
supernatant
transformating
angiotensin
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP15123480A
Other languages
Japanese (ja)
Inventor
Chikamitsu Okano
Masaaki Yamada
Eiichi Hasegawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Priority to JP15123480A priority Critical patent/JPS5774087A/en
Publication of JPS5774087A publication Critical patent/JPS5774087A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE: The extraction with a surfactant, the fractionation of the extract with a zinc salt and other operations are effected to separate and purify the A Tase from tissues of human placenta.
CONSTITUTION: Tissues of human placenta are homogenized together with an aqueous solution containing an anionic or nonionic surfactant and centrifuged into the precipitate and the supernatant. The supernatant is fractionated by salting out with a zinc salt such as zinc acetate or zinc sulfate to recover the resultant supernatant. It is adjusted in pH to 6W8, brought into contact with an anion exchanger equilibrated with a buffer solution of 6W8pH, eluted with an aqueous solution containing 0.5W2M salt and the eluate is treated with a protease for decomposition. Then, the product is subjected to gel filtration to isolate the fraction in which A Tase is active.
COPYRIGHT: (C)1982,JPO&Japio
JP15123480A 1980-10-27 1980-10-27 Separation and purification of placental enzyme transformating angiotensin (a tase) Pending JPS5774087A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15123480A JPS5774087A (en) 1980-10-27 1980-10-27 Separation and purification of placental enzyme transformating angiotensin (a tase)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15123480A JPS5774087A (en) 1980-10-27 1980-10-27 Separation and purification of placental enzyme transformating angiotensin (a tase)

Publications (1)

Publication Number Publication Date
JPS5774087A true JPS5774087A (en) 1982-05-10

Family

ID=15514174

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15123480A Pending JPS5774087A (en) 1980-10-27 1980-10-27 Separation and purification of placental enzyme transformating angiotensin (a tase)

Country Status (1)

Country Link
JP (1) JPS5774087A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59104555A (en) * 1982-11-08 1984-06-16 アボツト・ラボラトリ−ズ Method of measuring terminal deoxynucleotidyltransferase
US5168061A (en) * 1986-05-15 1992-12-01 Board Of Regents, The University Of Texas System Human chorionic peptidase-1
WO1998006829A1 (en) * 1996-08-12 1998-02-19 Fujisawa Pharmaceutical Co., Ltd. Methods for preparing bioreactors
US6271201B1 (en) 1993-07-15 2001-08-07 Board Of Regents, The University Of Texas System Methods for the selective regulation of placental prostanoids and inhibition of labor using IGF-I

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59104555A (en) * 1982-11-08 1984-06-16 アボツト・ラボラトリ−ズ Method of measuring terminal deoxynucleotidyltransferase
US5168061A (en) * 1986-05-15 1992-12-01 Board Of Regents, The University Of Texas System Human chorionic peptidase-1
US6271201B1 (en) 1993-07-15 2001-08-07 Board Of Regents, The University Of Texas System Methods for the selective regulation of placental prostanoids and inhibition of labor using IGF-I
WO1998006829A1 (en) * 1996-08-12 1998-02-19 Fujisawa Pharmaceutical Co., Ltd. Methods for preparing bioreactors

Similar Documents

Publication Publication Date Title
AU1577795A (en) Endotoxin reduction or removal process
NZ330484A (en) Isolation of lactoferrin especially recombinant lactoferrin from milk
ES8507535A1 (en) 7-Amino-1-cyclopropyl-6,8-difluoro-1,4-dihydro-4-oxo-3-quinoline-carboxylic acid, processes for their preparation and antibacterial agents containing them.
JPS5360291A (en) Ion exchange chromatography
KR890001581A (en) Tissue Factor Inhibitor
TODA et al. Chromatography of Taka-amylase A on diethylaminoethyl-cellulose column
IE871967L (en) Binding-factor-related polypeptides
AU2444795A (en) A process for separating milk clotting enzymes, and stable rennet compositions
JPS5774087A (en) Separation and purification of placental enzyme transformating angiotensin (a tase)
CA1283072C (en) Process for the isolation and separation of lysozyme and avidin from eggwhite
US2808362A (en) Preparation of hyaluronidase
DE69902639T2 (en) IMPROVED METHOD FOR PRODUCING FACTOR VIII
EP0291349A3 (en) Method of removing sodium dodecyl sulfate from sodium dodecyl sulfate solubilised protein solutions
EP1306383B1 (en) Method of purifying a calcium ion-binding protein
SU1138410A1 (en) Method for isolating thermostable placentar alkaline phosphatase
JPS57155991A (en) Preparation of superoxide dismutase derived from human placenta
US2406840A (en) Method of preparing pectase
JPS5577890A (en) Preparation of alcohol-resistant high-purity phycocyanin
GB2003887A (en) Isolation of peptides from urine
JPH0215192B2 (en)
DE3586197D1 (en) METHOD FOR CLEANING AND PASTEURIZING UROKINASE.
Liau et al. Isolation of Human Polymorphonudear Leukocyte Elastase by Chromatography on Immobilized Benzamidine
CA1065307A (en) PROCESS FOR THE PURIFICATION OF THE PREGNANCY-SPECIFIC .beta.1-GLYCOPROTEIN
RO103832B1 (en) Removal method of polyvalent cations from solutions of alkaline monochromate
FR2387240A1 (en) Pure human albumin recovery esp. from placental tissue - by pptn. of impurities with polyethylene glycol, for treating haemorrhage, shock etc.