JP2669457B2 - 工業微生物種中の分子クローニングおよび発現 - Google Patents
工業微生物種中の分子クローニングおよび発現Info
- Publication number
- JP2669457B2 JP2669457B2 JP59502729A JP50272984A JP2669457B2 JP 2669457 B2 JP2669457 B2 JP 2669457B2 JP 59502729 A JP59502729 A JP 59502729A JP 50272984 A JP50272984 A JP 50272984A JP 2669457 B2 JP2669457 B2 JP 2669457B2
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- Prior art keywords
- industrial
- bacillus
- host cell
- plasmid
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000037351 starvation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 description 1
- 229960001082 trimethoprim Drugs 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 108010082737 zymolyase Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Compounds Of Unknown Constitution (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP83201016.9 | 1983-07-06 | ||
| EP83201016 | 1983-07-06 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5269181A Division JP2664860B2 (ja) | 1983-07-06 | 1993-10-27 | 工業バチルス微生物種によるタンパク質又はポリペプチドの製造 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60501738A JPS60501738A (ja) | 1985-10-17 |
| JP2669457B2 true JP2669457B2 (ja) | 1997-10-27 |
Family
ID=8190976
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59502729A Expired - Fee Related JP2669457B2 (ja) | 1983-07-06 | 1984-07-06 | 工業微生物種中の分子クローニングおよび発現 |
| JP5269181A Expired - Fee Related JP2664860B2 (ja) | 1983-07-06 | 1993-10-27 | 工業バチルス微生物種によるタンパク質又はポリペプチドの製造 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5269181A Expired - Fee Related JP2664860B2 (ja) | 1983-07-06 | 1993-10-27 | 工業バチルス微生物種によるタンパク質又はポリペプチドの製造 |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0134048B2 (OSRAM) |
| JP (2) | JP2669457B2 (OSRAM) |
| AU (1) | AU570709B2 (OSRAM) |
| DE (1) | DE3480411D1 (OSRAM) |
| DK (1) | DK169996B1 (OSRAM) |
| ES (2) | ES8608579A1 (OSRAM) |
| FI (1) | FI85287C (OSRAM) |
| GR (1) | GR82200B (OSRAM) |
| IE (1) | IE58014B1 (OSRAM) |
| NZ (1) | NZ208806A (OSRAM) |
| WO (1) | WO1985000382A1 (OSRAM) |
Families Citing this family (103)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5624829A (en) * | 1984-07-03 | 1997-04-29 | Gist-Brocades, B.V. | Transformed industrial bacillus strains and methods for making and using them |
| CA1311429C (en) * | 1983-07-06 | 1992-12-15 | Vasantha Nagarajan | Vector for polypeptides in bacilli |
| EP0149241B1 (en) * | 1983-12-28 | 1991-03-27 | Agency Of Industrial Science And Technology | Dna base sequence containing regions involved in the production and secretion of a protein, recombinant dna including the whole or a part of the dna base sequence, and method of producing proteins by use of the recombinant dna |
| JPS60188093A (ja) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | 枯草菌に於ける形質発現プラスミド |
| IE58099B1 (en) * | 1984-06-06 | 1993-06-30 | Cpc International Inc | Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system |
| US4801537A (en) * | 1984-06-08 | 1989-01-31 | Genex Corporation | Vector for expression of polypeptides in bacilli |
| JPH0616711B2 (ja) * | 1985-09-27 | 1994-03-09 | メルシャン株式会社 | 高温で自律増殖するプラスミド |
| US5024943A (en) * | 1985-11-07 | 1991-06-18 | Gist-Brocades | Regulatory region cloning and analysis plasmid for bacillus |
| DK311186D0 (da) * | 1986-06-30 | 1986-06-30 | Novo Industri As | Enzymer |
| RU2091487C1 (ru) * | 1987-02-27 | 1997-09-27 | Гист-Брокейдс Н.В. | Способ получения штамма bacillus, содержащего две копии последовательности днк, кодирующей фермент с гидролазной активностью |
| ES2134186T3 (es) * | 1987-02-27 | 1999-10-01 | Genencor Int | Clonaje molecular y expresion de genes que codifican enzimas proteoliticas. |
| KR0127560B1 (ko) * | 1987-02-27 | 1997-12-29 | 마가렛트 에이.호른 | 단백질 분해 효소 코드화 유전자의 분자클로닝 및 발현 |
| DK0410498T3 (da) * | 1989-06-29 | 1999-03-22 | Genencor Int | Mutante mikrobielle alfa-amylaser med øget termisk, syre og/eller alkalisk stabilitet |
| DE4216246A1 (de) * | 1992-05-16 | 1993-11-18 | Solvay Enzymes Gmbh & Co Kg | Einfaches Verfahren zur stabilen chromosomalen Genamplifikation |
| GB9907461D0 (en) | 1999-03-31 | 1999-05-26 | King S College London | Neurite regeneration |
| US20050079573A1 (en) | 1998-12-23 | 2005-04-14 | Danisco A/S | Proteins |
| GB0020498D0 (en) | 2000-08-18 | 2000-10-11 | Sterix Ltd | Compound |
| CZ2003798A3 (cs) | 2000-09-21 | 2003-08-13 | Dsm N. V. | Polypeptid s aktivitou xylanázy, sekvence nukleové kyseliny kódující polypeptid, způsob přípravy a použití polypeptidu |
| CN1300328C (zh) | 2000-12-07 | 2007-02-14 | Dsmip资产有限公司 | 富含具有羧基末端脯氨酸残基的肽的蛋白质水解产物 |
| PT1326957E (pt) | 2000-12-07 | 2004-09-30 | Dsm Ip Assets Bv | Metodo para a prevencao ou reducao de turvacao em bebidas |
| GB0116453D0 (en) | 2001-07-05 | 2001-08-29 | Imp College Innovations Ltd | Method |
| CA2915148C (en) | 2002-03-29 | 2018-03-13 | Eugenio Ferrari | Enhanced protein expression in bacillus |
| ES2525873T3 (es) | 2002-06-07 | 2014-12-30 | Dsm Ip Assets B.V. | Método mejorado para la prevención o reducción del enturbiamiento en bebidas |
| TWI328612B (en) | 2002-07-25 | 2010-08-11 | Dsm Ip Assets Bv | Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12 |
| EP1625202A4 (en) | 2003-05-12 | 2010-10-20 | Genencor Int | NEW LIPOLYTIC ENZYME LIP1 |
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|---|---|---|---|---|
| JPS56128796A (en) * | 1980-02-15 | 1981-10-08 | Cpc International Inc | Genetically transformed microbe for mass production of amylolytic enzyme and its prescription |
| JPS56137896A (en) * | 1980-03-10 | 1981-10-28 | Cetus Corp | Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO159863C (no) * | 1980-01-07 | 1989-02-15 | Univ Rochester | Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme. |
| IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
| AU545912B2 (en) * | 1980-03-10 | 1985-08-08 | Cetus Corporation | Cloned heterologous jive products in bacillies |
| FI64813C (fi) * | 1980-12-31 | 1984-01-10 | Ilkka Antero Palva | Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer |
| CA1206108A (en) * | 1981-03-06 | 1986-06-17 | Shuichi Aiba | Process for transforming microorganism by means of plasmid introduction |
| BR8205954A (pt) * | 1981-10-14 | 1983-09-13 | Unilever Nv | Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos |
| JPS5889194A (ja) * | 1981-11-24 | 1983-05-27 | Ajinomoto Co Inc | 微生物によるｌ−トリプトフアンの製造法 |
| JPS58107192A (ja) * | 1981-12-18 | 1983-06-25 | Ajinomoto Co Inc | 発酵法によるｌ−ヒスチジンの製造法 |
-
1984
- 1984-07-06 DE DE8484200990T patent/DE3480411D1/de not_active Expired
- 1984-07-06 IE IE172684A patent/IE58014B1/en not_active IP Right Cessation
- 1984-07-06 JP JP59502729A patent/JP2669457B2/ja not_active Expired - Fee Related
- 1984-07-06 EP EP19840200990 patent/EP0134048B2/en not_active Expired - Lifetime
- 1984-07-06 NZ NZ20880684A patent/NZ208806A/xx unknown
- 1984-07-06 GR GR75238A patent/GR82200B/el unknown
- 1984-07-06 AU AU31510/84A patent/AU570709B2/en not_active Expired
- 1984-07-06 WO PCT/NL1984/000021 patent/WO1985000382A1/en not_active Ceased
- 1984-07-06 ES ES534102A patent/ES8608579A1/es not_active Expired
-
1985
- 1985-03-01 FI FI850845A patent/FI85287C/fi not_active IP Right Cessation
- 1985-03-05 DK DK101385A patent/DK169996B1/da not_active IP Right Cessation
- 1985-07-16 ES ES545256A patent/ES8609467A1/es not_active Expired
-
1993
- 1993-10-27 JP JP5269181A patent/JP2664860B2/ja not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56128796A (en) * | 1980-02-15 | 1981-10-08 | Cpc International Inc | Genetically transformed microbe for mass production of amylolytic enzyme and its prescription |
| JPS56137896A (en) * | 1980-03-10 | 1981-10-28 | Cetus Corp | Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria |
Non-Patent Citations (7)
| Title |
|---|
| Ａｍ．Ｓｏｃ．Ｍｉｃｒｏｂｉｏｌ．，ｐ．１０４−１０８（１９８１） |
| Ｂｉｏｌｏｇｉｃａｌ Ａｂｓｔｖａｃｔｓ，７３（１９８２）ｎｏ．３８８０５ |
| Ｂｉｏｌｏｇｉｃａｌ Ａｂｓｔｖａｃｔｓ，７３（１９８２）ｎｏ．５３３６８ |
| Ｊ．Ｂｕｃｔｅｒｉｏｌ．，１４７，ｐ．６２２−（１９８１） |
| Ｍｏｌ．ｇｅｎ．Ｇｅｎｅｔ．，１６８，ｐ．１１１−（１９７９） |
| Ｍｏｌ．ｇｅｎ．Ｇｅｎｅｔ．，１７６，ｐ．２３９−（１９７９） |
| Ｍｏｌ．ｇｅｎ．Ｇｅｎｅｔ．，１７７，ｐ．４５９−（１９８０） |
Also Published As
| Publication number | Publication date |
|---|---|
| DK101385A (da) | 1985-03-05 |
| DK169996B1 (da) | 1995-04-24 |
| FI85287C (fi) | 1992-03-25 |
| ES8609467A1 (es) | 1986-08-01 |
| FI850845A0 (fi) | 1985-03-01 |
| DE3480411D1 (en) | 1989-12-14 |
| EP0134048B2 (en) | 1996-08-14 |
| NZ208806A (en) | 1988-07-28 |
| FI85287B (fi) | 1991-12-13 |
| WO1985000382A1 (en) | 1985-01-31 |
| IE58014B1 (en) | 1993-06-16 |
| ES545256A0 (es) | 1986-08-01 |
| EP0134048A1 (en) | 1985-03-13 |
| JPS60501738A (ja) | 1985-10-17 |
| IE841726L (en) | 1985-01-06 |
| GR82200B (OSRAM) | 1984-12-13 |
| JP2664860B2 (ja) | 1997-10-22 |
| AU570709B2 (en) | 1988-03-24 |
| DK101385D0 (da) | 1985-03-05 |
| ES8608579A1 (es) | 1986-06-16 |
| EP0134048B1 (en) | 1989-11-08 |
| JPH06189783A (ja) | 1994-07-12 |
| AU3151084A (en) | 1985-02-07 |
| ES534102A0 (es) | 1986-06-16 |
| FI850845L (fi) | 1985-03-01 |
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| LAPS | Cancellation because of no payment of annual fees |