DE3480411D1 - Molecular cloning and expression in industrial microorganism species - Google Patents

Molecular cloning and expression in industrial microorganism species

Info

Publication number
DE3480411D1
DE3480411D1 DE8484200990T DE3480411T DE3480411D1 DE 3480411 D1 DE3480411 D1 DE 3480411D1 DE 8484200990 T DE8484200990 T DE 8484200990T DE 3480411 T DE3480411 T DE 3480411T DE 3480411 D1 DE3480411 D1 DE 3480411D1
Authority
DE
Germany
Prior art keywords
expression
molecular cloning
microorganism species
industrial microorganism
industrial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8484200990T
Other languages
German (de)
English (en)
Inventor
Johan Pieter Marinus Sanders
Den Berg Johannes Abel Van
Peter Michael Andreoli
Yvonne Johanna Vos
Ee Jan Hendrik Van
Leo J S M Mulleners
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke DSM NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8190976&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3480411(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Application granted granted Critical
Publication of DE3480411D1 publication Critical patent/DE3480411D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
DE8484200990T 1983-07-06 1984-07-06 Molecular cloning and expression in industrial microorganism species Expired DE3480411D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP83201016 1983-07-06

Publications (1)

Publication Number Publication Date
DE3480411D1 true DE3480411D1 (en) 1989-12-14

Family

ID=8190976

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8484200990T Expired DE3480411D1 (en) 1983-07-06 1984-07-06 Molecular cloning and expression in industrial microorganism species

Country Status (11)

Country Link
EP (1) EP0134048B2 (OSRAM)
JP (2) JP2669457B2 (OSRAM)
AU (1) AU570709B2 (OSRAM)
DE (1) DE3480411D1 (OSRAM)
DK (1) DK169996B1 (OSRAM)
ES (2) ES8608579A1 (OSRAM)
FI (1) FI85287C (OSRAM)
GR (1) GR82200B (OSRAM)
IE (1) IE58014B1 (OSRAM)
NZ (1) NZ208806A (OSRAM)
WO (1) WO1985000382A1 (OSRAM)

Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
DE3484346D1 (de) * 1983-12-28 1991-05-02 Agency Ind Science Techn Dns-basensequenz enthaltende regionen einbegriffen in der herstellung und sekretion eines proteins, rekombinante dns das ganze oder einen teil der dns-basensequenz enthaltend und verfahren zur herstellung von proteinen unter verwendung der rekombinanten dns.
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
IE58099B1 (en) * 1984-06-06 1993-06-30 Cpc International Inc Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
JPH0616711B2 (ja) * 1985-09-27 1994-03-09 メルシャン株式会社 高温で自律増殖するプラスミド
US5024943A (en) * 1985-11-07 1991-06-18 Gist-Brocades Regulatory region cloning and analysis plasmid for bacillus
DK311186D0 (da) * 1986-06-30 1986-06-30 Novo Industri As Enzymer
WO1988006623A1 (en) * 1987-02-27 1988-09-07 Gist-Brocades N.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
EP0283075B1 (en) * 1987-02-27 1999-05-12 Genencor International, Inc. Molecular cloning and expression of genes encoding proteolytic enzymes
BR8805647A (pt) * 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
ES2117625T3 (es) * 1989-06-29 1998-08-16 Genencor Int Alfa-amilasas microbianas mutantes con estabilidad termica, acida y/o alcalina incrementada.
DE4216246A1 (de) * 1992-05-16 1993-11-18 Solvay Enzymes Gmbh & Co Kg Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
DK1319079T3 (da) 2000-09-21 2013-01-07 Basf Se Talaromyces-xylanase
EP1464234A1 (en) 2000-12-07 2004-10-06 DSM IP Assets B.V. Method for the prevention or reduction of haze in beverages
WO2002045524A2 (en) 2000-12-07 2002-06-13 Dsm Ip Assets B.V. Prolyl endoprotease from aspergillus niger
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
EP2339017A3 (en) 2002-03-29 2011-10-12 Genencor International, Inc. Enhanced protein expression in bacillus
US8257760B2 (en) 2002-06-07 2012-09-04 Dsm Ip Assets B.V. Method for the prevention or reduction of haze in beverages
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
US20100129862A1 (en) 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
ES2755775T3 (es) 2005-11-28 2020-04-23 Dsm Ip Assets Bv Preparación de enzima que produce un sabor puro
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
CA2676686C (en) 2007-02-20 2015-12-15 Dsm Ip Assets B.V. Novel sialidase
US8779112B2 (en) 2007-03-12 2014-07-15 Danisco Us Inc. Modified protease propeptides
EP2126060B1 (en) 2007-03-22 2016-10-05 DSM IP Assets B.V. Novel lysyl oxidases
DK2152732T3 (da) 2007-05-10 2012-06-04 Danisco Us Inc Modificeret sekretionssystem til at øge ekspression af polypeptider i bakterier
GB0715751D0 (en) * 2007-08-13 2007-09-19 Tmo Renewables Ltd Thermophilic micro-organisms for ethanol production
EP2235176B1 (en) 2007-12-21 2017-06-21 Danisco US Inc. Enhanced protein production in bacillus
NZ587540A (en) 2008-03-28 2012-06-29 Danisco Us Inc Method for amplifying locus in bacterial cell
JP2012524542A (ja) 2009-04-24 2012-10-18 ダニスコ・ユーエス・インク 修飾されたプロ（ｐｒｏ）領域をもつプロテアーゼ
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
AR079338A1 (es) 2009-12-09 2012-01-18 Danisco Us Inc Variantes de proteasa de bacillus y acidos nucleicos que codifican dichas variantes
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
AR080886A1 (es) 2010-04-15 2012-05-16 Danisco Us Inc Composiciones y metodos que comprenden proteasas variantes
WO2011140364A1 (en) 2010-05-06 2011-11-10 Danisco Us Inc. Compositions and methods comprising subtilisin variants
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
US9856466B2 (en) 2011-05-05 2018-01-02 Danisco Us Inc. Compositions and methods comprising serine protease variants
WO2012151480A2 (en) 2011-05-05 2012-11-08 The Procter & Gamble Company Compositions and methods comprising serine protease variants
CN102296042B (zh) * 2011-08-26 2013-04-24 甘肃农业大学 地衣芽孢杆菌及用其制备凝乳酶冻干粉的方法
EP2751263A1 (en) 2011-08-31 2014-07-09 Danisco US Inc. Compositions and methods comprising a lipolytic enzyme variant
CN104024407A (zh) 2011-12-22 2014-09-03 丹尼斯科美国公司 包含脂解酶变体的组合物和方法
KR20150067336A (ko) 2012-10-12 2015-06-17 다니스코 유에스 인크. 지방분해 효소 변이체를 포함하는 조성물 및 방법
CN104781400A (zh) 2012-11-05 2015-07-15 丹尼斯科美国公司 包含嗜热菌蛋白酶变体的组合物和方法
WO2014100018A1 (en) 2012-12-19 2014-06-26 Danisco Us Inc. Novel mannanase, compositions and methods of use thereof
CN105492603B (zh) 2013-05-29 2022-06-03 丹尼斯科美国公司 新型金属蛋白酶
WO2014194117A2 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
EP3004314B1 (en) 2013-05-29 2018-06-20 Danisco US Inc. Novel metalloproteases
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US20160160197A1 (en) 2013-07-19 2016-06-09 Danisco Us Inc. Compositions and Methods Comprising a Lipolytic Enzyme Variant
DK3044313T3 (da) 2013-09-12 2020-02-17 Danisco Us Inc Sammensætninger og fremgangsmåder omfattende lg12-clade- proteasevarianter
EP3910057A1 (en) 2013-12-13 2021-11-17 Danisco US Inc. Serine proteases of the bacillus gibsonii-clade
EP3080262B1 (en) 2013-12-13 2019-02-06 Danisco US Inc. Serine proteases of bacillus species
WO2015102814A1 (en) 2013-12-31 2015-07-09 Danisco Us Inc. Enhanced protein expression
JP6585698B2 (ja) 2014-03-21 2019-10-02 ダニスコ・ユーエス・インク バチルス（Ｂａｃｉｌｌｕｓ）種のセリンプロテアーゼ
DK3207129T3 (da) 2014-10-17 2020-02-24 Danisco Us Inc Serinproteaser af bacillus-arten
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
WO2016069557A1 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases of bacillus species
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DK3212781T3 (da) 2014-10-27 2019-12-16 Danisco Us Inc Serinproteaser
WO2016069569A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
WO2016099917A1 (en) 2014-12-16 2016-06-23 Danisco Us Inc Enhanced protein expression
CN107278230B (zh) 2014-12-19 2021-10-29 丹尼斯科美国公司 增强的蛋白质表达
CN107454914B (zh) 2015-03-12 2021-09-21 丹尼斯科美国公司 包含lg12进化枝蛋白酶变体的组合物和方法
RU2733987C2 (ru) 2015-05-13 2020-10-09 ДАНИСКО ЮЭс ИНК. Варианты протеазы клады aprl и их применения
WO2016205755A1 (en) 2015-06-17 2016-12-22 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
EP3310913A1 (en) 2015-06-17 2018-04-25 Danisco US Inc. Proteases with modified propeptide regions
US20190194636A1 (en) 2016-05-03 2019-06-27 Danisco Us Inc Protease variants and uses thereof
EP3452584B1 (en) 2016-05-05 2021-01-06 Danisco US Inc. Protease variants and uses thereof
CN119307476A (zh) 2016-05-31 2025-01-14 丹尼斯科美国公司 蛋白酶变体及其用途
MX2018015559A (es) 2016-06-17 2019-06-06 Danisco Us Inc Variantes de proteasa y sus usos.
US20200392477A1 (en) 2016-12-21 2020-12-17 Danisco Us Inc. Protease variants and uses thereof
US11946081B2 (en) 2016-12-21 2024-04-02 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
US12415996B2 (en) 2018-06-19 2025-09-16 Danisco Us Inc. Subtilisin variants
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
WO2020046613A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
US20220220419A1 (en) 2019-05-24 2022-07-14 Danisco Us Inc Subtilisin variants and methods of use
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US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
US20240294888A1 (en) 2021-06-30 2024-09-05 Danisco Us Inc. Variant enzymes and uses thereof
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EP4448708A1 (en) 2021-12-16 2024-10-23 The Procter & Gamble Company Fabric and home care composition comprising a protease
US20240166973A1 (en) 2021-12-16 2024-05-23 The Procter & Gamble Company Automatic dishwashing composition comprising a protease
EP4448751A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and methods of use
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CN118679252A (zh) 2021-12-16 2024-09-20 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
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CA3265718A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc VARIANTS OF SUBTILISINE AND ASSOCIATED PROCESSES
JP2025530749A (ja) 2022-09-02 2025-09-17 ダニスコ・ユーエス・インク 洗剤組成物及びそれに関連する方法
CN120303400A (zh) 2022-11-09 2025-07-11 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2024163695A1 (en) 2023-02-01 2024-08-08 The Procter & Gamble Company Detergent compositions containing enzymes
EP4658776A1 (en) 2023-02-01 2025-12-10 Danisco US Inc. Subtilisin variants and methods of use
CN120712348A (zh) 2023-03-06 2025-09-26 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
ZA81424B (en) * 1980-02-15 1982-02-24 Cpc International Inc Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
JPS56137896A (en) * 1980-03-10 1981-10-28 Cetus Corp Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria
AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1206108A (en) * 1981-03-06 1986-06-17 Shuichi Aiba Process for transforming microorganism by means of plasmid introduction
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
JPS5889194A (ja) * 1981-11-24 1983-05-27 Ajinomoto Co Inc 微生物によるｌ−トリプトフアンの製造法
JPS58107192A (ja) * 1981-12-18 1983-06-25 Ajinomoto Co Inc 発酵法によるｌ−ヒスチジンの製造法

Also Published As

Publication number Publication date
EP0134048B2 (en) 1996-08-14
IE841726L (en) 1985-01-06
ES8609467A1 (es) 1986-08-01
AU3151084A (en) 1985-02-07
DK101385A (da) 1985-03-05
FI850845A0 (fi) 1985-03-01
JPS60501738A (ja) 1985-10-17
DK169996B1 (da) 1995-04-24
FI85287B (fi) 1991-12-13
JP2669457B2 (ja) 1997-10-27
EP0134048B1 (en) 1989-11-08
FI850845L (fi) 1985-03-01
DK101385D0 (da) 1985-03-05
ES534102A0 (es) 1986-06-16
AU570709B2 (en) 1988-03-24
WO1985000382A1 (en) 1985-01-31
EP0134048A1 (en) 1985-03-13
IE58014B1 (en) 1993-06-16
GR82200B (OSRAM) 1984-12-13
JP2664860B2 (ja) 1997-10-22
ES545256A0 (es) 1986-08-01
FI85287C (fi) 1992-03-25
ES8608579A1 (es) 1986-06-16
NZ208806A (en) 1988-07-28
JPH06189783A (ja) 1994-07-12

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